Analysis method for physiological traits of tribute bananas under magnesium and zinc composite stress

Abstract

The invention discloses a method for analyzing physiological traits of tribute bananas under magnesium and zinc composite stress, which comprises the following steps: S1, cleaning soil at the roots of seedling plants, and putting seedlings into clear water for nutrient starvation cultivation for 12 hours; s2, transplanting the seedling plant into a culture cup containing vermiculite for culture; s3, culturing the plants by using an improved Hoagland nutrient solution, and setting a two-factor three-level orthogonal test; s4, measuring various data of the plants in the culture process, and processing, comparing and analyzing the data. According to the invention, an important scientific basis is provided for formulating an effective agricultural management strategy to relieve or eliminate adverse effects of the stress on crop growth.

Description

Technical Field

[0001] The invention relates to the technical field of banana planting, and in particular to an analysis method for physiological traits of bananas under the combined stress of magnesium and zinc. Background Art

[0002] Banana is a tropical fruit of the genus Musa in the family Musaceae. It is the fourth largest food crop in the world after rice, wheat and corn, and is also the fresh fruit with the largest fruit trade volume. my country has been planting bananas for thousands of years. According to data from the National Bureau of Statistics, the cultivated area of ​​bananas reached 5.05 million mu in 2022, and the banana production in 2023 was about 11.7028 million tons. "Gongjiao", also known as Emperor Banana, Millet Banana, Golden Banana, etc., is one of the AA-type diploid bananas for daily consumption. Emperor Banana was introduced from Indonesia to Xinglong, Hainan Province by Li Muqing, an overseas Chinese in my country, in 1963 for promotion and cultivation. It is currently planted in Hainan, Guangdong and Guangxi. Emperor Banana is a tropical specialty fruit tree that requires a lot of water and fertilizer, and likes high temperature and high humidity. The field water holding capacity needs to be between 60% and 80%; the pH value should be between 6.5 and 7.0, the organic matter content should be above 2%, and the demand for nutrients such as nitrogen, potassium, calcium, magnesium and phosphorus is relatively large.

[0003] Plant growth morphology covers the various morphological characteristics and changing patterns exhibited by plants during their growth and development. These morphological characteristics not only reflect the growth status of plants, but are also closely related to their physiological functions, ecological environment and genetic characteristics. In recent years, researchers have used chlorophyll fluorescence technology as one of the diagnostic tools for plant growth conditions, and it has been widely used in early monitoring of various types of stresses such as biotic and abiotic stresses in plants. At present, there are not many studies on the effects of mineral elements on photosynthetic characteristics after trace element deficiency in bananas. Magnesium and zinc are important trace elements in plant photosynthesis, and their deficiency or excess will have an important impact on plant photosynthetic characteristics. Magnesium stress can reduce chlorophyll content, inhibit photosynthetic rate, interfere with product transport, etc., while zinc stress can inhibit photosynthesis, interfere with plastid exchange between mitochondria and chloroplasts, resulting in slower plant growth and affecting yield. Excessive zinc concentration can interfere with the synthesis of plant chlorophyll, hinder electron transfer in the photosystem, inhibit the Hill reaction, and thus affect plant photosynthesis. Intracellular O under zinc pollution 2-Increased concentration will lead to increased membrane lipid peroxidation and increased cell membrane permeability. Chlorophyll is one of the most important pigments in nature for the absorption, transmission and conversion of light energy. Its metabolic level is related not only to plant germplasm, but also to the interaction of environmental factors. Plant chlorophyll fluorescence is a very important physiological indicator that can reflect the efficiency of photosynthesis and the response of plants to environmental stress. Compared with other ecophysiological methods (such as gas exchange methods), the advantage of measuring different components of chlorophyll fluorescence characteristics of intact leaves is that these fluorescence methods are very fast. In addition, they are sensitive and non-destructive. In summary, it is of great significance to study the growth morphology and photosynthetic characteristics of banana seedlings under magnesium and zinc stress. Summary of the invention

[0004] The purpose of the present invention is to provide an analysis method for the physiological traits of Gongjiao under the combined stress of magnesium and zinc. By setting different concentrations of magnesium and zinc treatments, the growth status, photosynthetic characteristics, chlorophyll fluorescence parameters and other data of Gongjiao seedlings under various treatment conditions are systematically evaluated.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The method for analyzing the physiological traits of Gongjiao under the combined stress of magnesium and zinc provided by the present invention specifically comprises the following steps:

[0007] S1. Clean the soil around the roots of the seedlings and place the seedlings in clean water for 12 hours of nutrient starvation cultivation;

[0008] S2, transplanting the seedlings into a culture cup containing vermiculite for cultivation;

[0009] S3, the plants were cultured with modified Hoagland nutrient solution, and an orthogonal experiment with two factors and three levels was set up;

[0010] S4. Measure various data of the plants during the cultivation process, and process, compare and analyze the data.

[0011] Furthermore, in step S1, the seedling plant is Musa spp., AA, meets the first-level seedling planting standard, has more than 5 rooted leaves, and is more than 15 cm in height.

[0012] Furthermore, in step S1, the water temperature in the clean water is maintained at 20-25°C for culturing.

[0013] Furthermore, in step S3, the formula of the modified Hoagland nutrient solution is: KNO3 5mmol·L -1 , Ca(NO3)2 5mmol·L -1 , MgSO4 2mmol·L -1 , KH2PO4 1mmol·L-1 , H3BO30.025mmol·L -1 ,MnSO4·4H2O 0.002mmol·L -1 , ZnSO4·7H2O 0.002mmol·L -1 , CuSO4·5H2O 0.0005mmol·L -1 , H2MoO4·4H2O 0.0005mmol·L -1 , EDTA-Fe0.00025mmol·L -1 .

[0014] Further, in step S3, two factors in the two factors and three levels are magnesium and zinc, and the content of magnesium and zinc in the three levels are 0%, 50%, and 100%, respectively.

[0015] Furthermore, in step S3, the modified Hoagland nutrient solution is watered once every 5 days, 50 ml each time, and the culture is carried out indoors at a culture temperature of 25-30°C.

[0016] Furthermore, Mg-Zn interactive stress affected the growth parameters and photosynthetic characteristics of seedlings.

[0017] Based on the above technical solution, the embodiments of the present invention can at least produce the following technical effects:

[0018] The analytical method for the physiological traits of Gongjiao under magnesium and zinc combined stress provided by the present invention deepens the understanding of the magnesium and zinc stress mechanism, and also provides an important scientific basis for formulating effective agricultural management strategies to alleviate or eliminate the adverse effects of such stress on crop growth. Further exploration of the absorption, transport, distribution and regulation mechanism of magnesium and zinc in plants and the development of nutritional management and cultivation techniques for specific stress environments will be the key direction for promoting sustainable agricultural development. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0020] Figure 1 This is the growth dynamics of Gongjiao seedlings plant height under different treatments;

[0021] Figure 2 The stem thickness of Gongjiao seedlings was different for different treatments;

[0022] Figure 3The total root length of Gongjiao seedlings under different treatments;

[0023] Figure 4 The leaf length and width of Gongjiao seedlings were different under different treatments;

[0024] Figure 5 Chlorophyll of Gongjiao seedlings treated with different methods;

[0025] Figure 6 Photosynthetic parameters of Gongjiao seedlings under different treatments. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in this field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0027] 1 Materials and methods

[0028] 1.1 Test materials

[0029] Starting from March 7, 2024, banana seedlings were cultivated in vermiculite pots. The specifications of the culture cups were 15cm×13cm, and the culture cups were filled with vermiculite. The test plant Emperor Banana, also known as Gongjiao (Musa spp., AA), is based on the first-level seedling nursery standard (NY / T357-1999 Banana Tissue Culture Seedlings), with more than 5 rooted leaves, a height of more than 15cm, and consistent growth status. Banana seedlings with consistent size and leaf health and no pests and diseases were selected as experimental materials. Before transplanting, in order to remove the soil at the roots without damaging the root system, they should be carefully washed with clean water. These banana seedlings were placed in clean water for 12 hours of "nutrient starvation" cultivation, and then they were transplanted into culture cups containing vermiculite. All cultivated banana seedlings will be used to measure different indicators.

[0030] Nutrient starvation: Remove the root attachments of the first-level seedlings and place all the test materials in clean water for 12 hours. The clean water can be tap water. The main function is to eliminate the imbalance of root nutrients between seedlings and reduce experimental errors. The water temperature is maintained at 20-25℃. After 12 hours, conduct potted experiments uniformly.

[0031] 1.2 Experimental design

[0032] This experimental design adopts a potted experiment, watered with Hoagland nutrient solution, with slight modifications. The experiment is a 2-factor 3-level orthogonal experiment, of which 2 factors are magnesium and zinc; 3 levels are 0%, 50%, and 100%. There are 9 treatments in the experiment, namely M1Z1, M1Z2, M1Z3, M2Z1, M2Z2, M2Z3, M3Z1, M3Z2, and M3Z3 (CK), where M represents magnesium, Z represents zinc, and 1, 2, and 3 represent the content of the element as 0%, 50%, and 100%, respectively. The nutrient solution is watered once every 5 days, 50 ml each time. To ensure that the vermiculite remains in a "dry and wet" state. The experiment was conducted from March 7, 2024 to July 31, 2024. The location is located in the Physiological Cultivation Laboratory of the Haikou Experimental Station of the Chinese Academy of Tropical Agricultural Sciences. It is cultivated indoors, with sufficient light and suitable temperature. The average daily temperature is about 27°C, and the temperature is suitable. Plant growth lights are used for lighting throughout the day to ensure sufficient light. Water every 3 to 4 days, about 30 ml per plant, to ensure normal growth of the seedlings.

[0033] Table 1 Experimental design 2 factors 3 levels

[0034]

[0035] Note: 1 means 0 ml of the element is required per liter of nutrient solution; 2 means 1 ml of the element is required per liter of nutrient solution; 3 means 2 ml of the element is required per liter of nutrient solution, which is the normal requirement.

[0036] Table 2 Experimental orthogonal design

[0037] serial number magnesium Zinc Representation method 1 1 1 M1Z1 2 1 2 M1Z2 3 1 3 M1Z3 4 2 1 M2Z1 5 2 2 M2Z2 6 2 3 M2Z3 7 3 1 M3Z1 8 3 2 M3Z2 9 3 3 M3Z3

[0038] Note: M3Z3 is the normal treatment, i.e. the control group.

[0039] Table 3 Composition of improved Hoagland nutrient solution

[0040]

[0041]

[0042] 1.3 Experimental data determination

[0043] Plant height, stem diameter, and leaf length and width were measured on the 0th day, and then measured every 15 days until 120 days, for a total of 9 times; 3 plants were selected as replicates for each treatment for each measurement, and the measurements were performed using a ruler and a vernier caliper. The roots were scanned using a scanner (EPSON-V800) produced by EPSON Corporation of Japan, and the total root length was analyzed using WinRHIZO software.

[0044] Three representative plants from different treatments were selected on the 0th, 30th, 60th, 90th, and 120th days, and the measurements were carried out from 9:00 to 11:00 a.m. on a clear and windless day (the penultimate complete leaf of the seedling). The Li-6800 photosynthetic meter produced by Li-COR Company of the United States was used, and the red and blue light source leaf chamber was selected. The fixed light intensity of the banana seedlings was set to 1500 μmol·m -2 ·s -1 , set the open gas line CO2 concentration to 400μmol·L -1 , measured photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr).

[0045] The number of fluorescence kinetic parameters was measured at the same time as the photosynthetic parameters. PAM-2500 produced by Zequan Technology Co., Ltd. was used for measurement. Three plants were selected for each treatment. The seedlings to be measured were dark-adapted for 20 minutes before measurement. Before each measurement, the fluorescence Ft when only the measuring light was turned on should be between 200-400 by adjusting the "Gain", measuring light intensity or the distance between the optical fiber and the sample. Subsequently, Fv / Fm, Y(II) and NPQ were measured.

[0046] Using a chlorophyll meter (SPAD-502) produced by Konica Minolta Co., Ltd. of Japan, the second to last complete leaf was selected for measurement every 15 days, and 3 plants were selected for each treatment.

[0047] 1.4 Analytical methods

[0048] SPSS and Excel were used to process the measured data, and Origin was used to draw graphs for all processed data.

[0049] 2 Results and analysis

[0050] 2.1 Growth morphology

[0051] 2.1.1 Plant height

[0052] pass Figure 1It can be concluded that under different treatment conditions, the plant height of Gongjiao seedlings showed an overall upward trend. On the 30th day of growth, the plant height of the M1Z1 treatment group was significantly lower than that of the other treatment groups. By the 120th day of growth, the plant heights of Gongjiao seedlings in the M1Z1, M1Z2, M1Z3, M2Z1, M2Z2, M2Z3, M3Z1, and M3Z2 treatment groups were 29.67cm, 29.80cm, 27.83cm, 30.75cm, 31.70cm, 32.75cm, 30.00cm, and 31.50cm, respectively, while the plant height of the normal treatment group was 32cm, indicating that the complete lack of magnesium and zinc had a certain effect on the plant height of Gongjiao seedlings. Although the plant height of other treatment groups was also lower than that of the normal treatment group, the gap between the M3Z2 treatment group and the normal treatment group was small. However, on the 90th day of growth, the plant height of the M1Z3 treatment group was lower than that of the M1Z1 treatment group, which may be due to the combined effects of nutrients, plant physiological responses, interference from other environmental factors, etc. Based on the above results, it can be concluded that under magnesium and zinc stress, the plant height of Gongjiao seedlings will be significantly inhibited, and the effect of zinc stress on plant height is more significant than that of magnesium stress.

[0053] 2.1.2 Stem diameter

[0054] according to Figure 2 The data analysis showed that the stem thickness of Gongjiao seedlings showed a clear growth trend. Specifically, during the 15th to 30th day of seedling growth, the growth rate of stem thickness increased significantly, showing a relatively rapid growth period. However, during the growth process from the 30th to the 120th day, the growth rate of stem thickness gradually slowed down, showing a relatively gentle upward trend. It is worth noting that under the 9 different treatment conditions measured, the growth of stem thickness of Gongjiao seedlings did not show significant statistical differences. This result shows that within the scope of the study, the stress conditions of magnesium and zinc have a relatively limited effect on the development of stem thickness of Gongjiao seedlings, that is, these elements may not be the dominant factors affecting the growth of stem thickness at this stage.

[0055] 2.1.3 Root growth

[0056] Depend on Figure 3It can be seen that in the early growth stage of Gongjiao seedlings, the total root length under different treatment conditions did not show significant differences, indicating that at this stage, the effects of magnesium and zinc supply on root expansion have not yet become prominent. During the 20-40 day growth period, the root length of the M3Z3 treatment was significantly longer than that of other treatments. By the 120th day of growth, after the accumulation of longer-term stress effects, the difference in root length between different treatments was further amplified. Specifically, the root length of Gongjiao seedlings under normal treatment reached 37.94 cm, while the total root length of magnesium and zinc deficiency treatments was 34.46 cm, indicating that the lack of magnesium and zinc significantly limited the length of the root system. In summary, magnesium and zinc stress conditions have a significant effect on the root length of Gongjiao seedlings, and this effect intensifies with the deepening of stress. Appropriate magnesium and zinc supply is essential for promoting normal growth and development of the root system.

[0057] 2.1.4 Leaf growth

[0058] Figure 4 The data analysis revealed the growth trends of leaf length and leaf width of Gongjiao seedlings under different treatment conditions. In general, seedlings in all nine treatment groups showed an increasing trend in leaf length and leaf width, but there were differences in the growth patterns. Specifically, the growth trend of leaf length was more significant, while the growth of leaf width was relatively slow. Further observations showed that the leaf length growth rate of the three treatment groups M1Z1, M1Z2, and M1Z3 was relatively slow. In contrast, the leaf length and leaf width of the M2Z1, M2Z2, and M2Z3 treatment groups decreased to varying degrees compared with the normal treatment. The leaf length of the M3Z1 and M3Z2 treatment groups did not show significant differences compared with the normal treatment, indicating that under these treatment conditions, magnesium may have been maintained at a level that is more favorable for leaf growth, while changes in zinc have relatively little effect on leaf length. This finding emphasizes the important role of magnesium in the leaf growth of Gongjiao seedlings. Although zinc may also have a certain effect on leaf growth, its effect may be limited.

[0059] 2.2 Photosynthetic characteristics

[0060] 2.2.1 Chlorophyll

[0061] according to Figure 5Data analysis shows that the changing trend of chlorophyll content in Gongjiao seedlings during their growth process presents a stage-by-stage characteristic. In the early stage of stress (0-30 days), the chlorophyll content increased significantly, which may reflect the process of seedlings adapting to the new environment and enhancing their photosynthesis capacity. However, in the middle stage (30-60 days), the chlorophyll content showed a downward trend, which may be related to the transition of plant growth stages, changes in nutrient absorption and utilization, or fluctuations in environmental factors. In the late stage of stress (60-120 days), the chlorophyll content rebounded again, indicating that the photosynthetic capacity of the plant was restored and improved after a period of adaptation. On the 60th day of growth, the chlorophyll content of Gongjiao seedlings under normal treatment conditions was significantly higher than that of each stress treatment group, especially the three treatments of M1Z1, M1Z2, and M1Z3, whose chlorophyll content was lower than that of other treatments. In addition, a similar pattern was observed at the end of the growth period, that is, the chlorophyll content under stress treatment was generally lower than that under normal treatment, further confirming the adverse effects of magnesium and zinc stress on chlorophyll content.

[0062] 2.2.2 Photosynthetic parameters

[0063] Depend on Figure 6 It can be seen that during the growth of Gongjiao seedlings, its physiological and ecological characteristics showed a specific trend of change. With the growth of seedlings, the net photosynthetic rate and intercellular carbon dioxide concentration showed an increasing trend, which indicated that the photosynthetic capacity was gradually increasing, and the accumulated carbon dioxide concentration between cells was also increasing, which may be the result of the dynamic balance between photosynthesis demand and stomatal conductance regulation. However, compared with the normal treatment conditions (M3Z3), the Gongjiao seedlings under stress treatment (M1Z1, M1Z2, M1Z3, M2Z1, M2Z2, M2Z3, M3Z1, M3Z2) showed a similar growth trend, but their net photosynthetic rate and transpiration rate were significantly lower than the normal level, which indicated that magnesium and zinc stress had an adverse effect on the photosynthesis and water use efficiency of seedlings. On the other hand, stomatal conductance showed a downward trend during the growth of Gongjiao seedlings. The intercellular carbon dioxide concentration of Gongjiao seedlings under stress treatment was higher than that under normal treatment. This may be due to the partial closure or functional impairment of stomata caused by stress, which hindered the gas exchange between the inside of the leaves and the outside world, resulting in the accumulation of carbon dioxide between cells. Photosynthetic parameters effectively reflect the photosynthetic capacity of plants under stress.

[0064] 2.2.3 Chlorophyll fluorescence parameters

[0065] Under normal treatment (M3Z3), the Fv / Fm of Gongjiao seedlings changed little, and under complete magnesium and zinc stress (M1Z1), this parameter decreased significantly. As the degree of stress decreased, the Fv / Fm value changed less (Table 4). Y(II) reflects the actual light energy conversion efficiency of leaves under light. From 0 to 60 days of stress, the Y(II) value of Gongjiao seedlings showed an increasing trend, indicating that the actual photochemical quantum efficiency of Gongjiao seedlings under mild stress showed a stable growth trend. From 90 to 120 days of stress, except for the normal treatment, the Y(II) values ​​of Gongjiao seedlings under other stress treatments showed a downward trend. Under normal treatment, the actual photochemical quantum efficiency of Gongjiao seedlings was 0.23, while under M1Z1 treatment it was only 0.06, indicating that magnesium and zinc stress would affect the Y(II) value of Gongjiao seedlings, and the longer the stress time, the greater the impact on the value (Table 5). Under normal treatment, the NPQ value fluctuated within a relatively stable range. Under stress treatment, Gongjiao seedlings dissipated excess light energy by increasing NPQ values, thereby protecting the photosynthetic apparatus from light damage. Therefore, from 0 to 60 days, the NPQ values ​​of Gongjiao seedlings showed an upward trend. It reached a peak on the 90th day of stress and then declined (Table 6). In summary, chlorophyll fluorescence parameters can be used as an effective probe to detect whether plants are under stress.

[0066] Table 4 Fv / Fm values ​​under different stress treatments

[0067]

[0068] Table 5 Y(II) values ​​under different stress treatments

[0069]

[0070]

[0071] Table 6 NPQ values ​​under different stress treatments

[0072]

[0073] Under the condition of increasing magnesium fertilizer, the average plant height and stem circumference of banana sprayed for a long time increased compared with the control treatment, and magnesium-zinc stress significantly reduced the plant height of seedlings. When plants are subjected to heavy metal Zn stress, plant growth is restricted, leaves lose green and wilt, branching ability becomes poor, and growth and development are abnormal, but the effect on the stem thickness of seedlings is small. This may be because the lack or excess of magnesium and zinc will affect the cell division and elongation of plants. In addition, the growth of leaves and roots is also significantly affected by magnesium-zinc stress. Magnesium-zinc stress reduces the chlorophyll content of seedling leaves, which directly affects the photosynthetic capacity of plants. In terms of photosynthetic parameters, magnesium-zinc stress leads to a decrease in the net photosynthetic rate, transpiration rate and stomatal conductance of seedlings. However, the intercellular carbon dioxide concentration is higher than that of normal treatment, which may be due to the reduction of stomatal conductance limiting the diffusion of CO2. At the same time, the decrease in Fv / Fm, Y(II) and NPQ values ​​indicates that the light energy conversion efficiency and non-photochemical quenching ability of photosystem II are negatively affected. The reaction center of PSII is an important place for absorbing, transmitting and converting light energy. Chlorophyll fluorescence, as an effective probe of photosynthesis, can evaluate the function of PSII by measuring its various properties. These results show that magnesium-zinc interaction stress has a series of negative effects on the photosynthesis and growth of Gongjiao seedlings. It is necessary to select varieties that adapt to each nutrient condition in the soil to achieve the best potential yield.

[0074] Based on the above analysis, the magnesium-zinc interaction stress had a significant and far-reaching effect on the growth parameters and photosynthetic characteristics of Gongjiao seedlings. Specifically, the stress condition caused a significant decrease in the plant height of the seedlings, and this morphological change directly reflected the overall growth inhibition of the plant. Furthermore, the content of chlorophyll, a key pigment for photosynthesis, was significantly reduced under stress treatment, which directly weakened the seedlings' ability to absorb and convert light energy, thereby affecting the efficiency of photosynthesis. At the same time, the decrease in net photosynthetic rate was a direct reflection of the effect of magnesium-zinc interaction stress on the photosynthesis of Gongjiao seedlings, indicating that the photosynthetic production capacity of seedlings was severely limited under stress. In addition, the decrease in transpiration rate and stomatal conductance may reflect the water-saving strategy adopted by seedlings in response to stress, but this also indirectly affected the gas exchange between leaves and the atmosphere, including the absorption of carbon dioxide, leading to an abnormal increase in intercellular carbon dioxide concentration. This series of physiological and biochemical changes together reveal the negative effects of magnesium-zinc interactive stress on photosynthesis of Gongjiao seedlings. This effect is achieved through multiple pathways such as reducing the content of photosynthetic pigments and affecting stomatal behavior, ultimately limiting the normal growth and development of the plants.

[0075] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements fall within the scope of the present invention to be protected. The scope of the present invention to be protected is defined by the attached claims and their equivalents.

Claims

1. A method for analyzing the physiological traits of Gongjiao under combined stress of magnesium and zinc, characterized in that: The specific steps include: S1. Clean the soil around the roots of the seedlings and place the seedlings in clean water for 12 hours of nutrient starvation cultivation; S2, transplanting the seedlings into a culture cup containing vermiculite for cultivation; S3, the plants were cultured with modified Hoagland nutrient solution, and an orthogonal experiment with two factors and three levels was set up; S4. Measure various data of the plants during the cultivation process, and process, compare and analyze the data.

2. The method for analyzing the physiological traits of Gongjiao under the combined stress of magnesium and zinc according to claim 1, characterized in that: In step S1, the seedling plant is a Musa spp., AA, which meets the first-level seedling planting standard, has more than 5 rooted leaves, and is more than 15 cm in height.

3. The method for analyzing the physiological traits of Gongjiao under the combined stress of magnesium and zinc according to claim 1, characterized in that: In step S1, the water temperature in the clean water is maintained at 20-25°C for cultivation.

4. The method for analyzing physiological traits of Gongjiao under combined stress of magnesium and zinc according to claim 1, characterized in that: In step S3, the formula of the modified Hoagland nutrient solution is: KNO3 5mmol·L -1 , Ca(NO3)2 5mmol·L -1 ,MgSO42mmol·L -1 , KH2PO4 1mmol·L -1 , H3BO30.025mmol·L -1 ,MnSO4·4H2O 0.002mmol·L -1 , ZnSO4·7H2O 0.002mmol·L -1 , CuSO4·5H2O 0.0005mmol·L -1 , H2MoO4·4H2O 0.0005mmol·L -1 , EDTA-Fe0.00025mmol·L -1 .

5. The method for analyzing physiological traits of Gongjiao under combined stress of magnesium and zinc according to claim 1, characterized in that: In step S3, two factors in the two factors and three levels are magnesium and zinc, and the content of magnesium and zinc in the three levels are 0%, 50%, and 100%, respectively.

6. The method for analyzing physiological traits of Gongjiao under combined stress of magnesium and zinc according to claim 1, characterized in that: In step S3, the modified Hoagland nutrient solution is watered once every 5 days, 50 ml each time, and the culture is carried out indoors at a culture temperature of 25-30°C.

7. The method for analyzing physiological traits of Gongjiao under combined stress of magnesium and zinc according to claim 1, characterized in that: Magnesium-zinc interactive stress affects growth parameters and photosynthetic characteristics of seedlings.

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