Microfluidic detection card capable of storage and sequential acquisition

Abstract

The invention provides a micro-fluidic detection card capable of storage and sequential acquisition, which is sequentially laminated from bottom to top: a substrate layer, a sensing layer and a micro-fluidic chip layer, and the sensing layer comprises a sensing unit; a plurality of detection cavities are formed between the sensing layer and the micro-fluidic chip layer; the sensing unit is integrated in each detection cavity and is also suitable for being connected with a detection serial port; the micro-fluidic chip layer is connected with a central liquid storage cavity, and is also provided with an inlet and an outlet which are communicated with the central liquid storage cavity, and a micro-channel which is sequentially communicated with the inlet, the central liquid storage cavity and the outlet, and the micro-channel sequentially forms a positive guide Tesla valve and a negative guide Tesla valve in the flow direction from the inlet to the outlet; according to the invention, a plurality of detection cavities are designed on the improved Tesla valve structure, micro-liquids refluxed at different times can be separated by using the flow blocking characteristic of the reverse guide Tesla valve, and micro-liquid data at different times can be detected and contrastively analyzed, so that the micro-liquid detection device is suitable for micro-liquid detection.

Description

Technical Field

[0001] The invention relates to the field of micro-electromechanical processing technology and microfluidic analysis and detection technology, and in particular to a microfluidic detection card capable of storing and sequentially collecting data. Background Art

[0002] In the early 1990s, Manz et al. proposed the concept of a micro-total analysis system based on micro-electromechanical processing technology, also known as lab-on-a-chip, which refers to the integration of multiple components involving biochemical micro-operations on a chip of several square centimeters or even smaller through specific technologies based on microfluidic technology. Through the special design of the microchannel system, the high degree of integration can make it easier for the mixture to pass through each unit through sampling, mixing, extraction, separation, detection and other steps. Microfluidic chips have the characteristics of flexible combination of multiple functional units, large-scale integration to achieve high-throughput screening, small size, and small sample size. According to the detection requirements, micropumps, microvalves, mixing devices, drivers and other functional units can be designed and prepared and integrated on microchips to make high-precision two-dimensional or more complex three-dimensional chip integrated systems.

[0003] Since microfluidics and miniaturization technology are closely related, while reducing the reaction volume, the absolute number of analytes is also reduced accordingly, so that smaller compounds can be analyzed and identified from the overall substance. Therefore, high-sensitivity detection technology is indispensable. Microfluidic chip detectors are important functional components of microfluidic chip analysis and detection systems. Their function is to determine the composition and content of relevant samples separated or processed by the microfluidic analysis system. The detectors used in microfluidic detection systems mainly include: electrochemical detectors, mass spectrometer detectors and optical detectors. Among them, electrochemical and optical detection are suitable for the needs of miniaturization and integration of microfluidic chip systems. Microelectrodes or optical paths can be easily integrated through MEMS technology. Summary of the invention

[0004] In view of the above-mentioned shortcomings of the prior art, an object of the present invention is to provide a microfluidic detection card capable of storage and sequential collection, so as to solve the problem in the prior art that sequential detection cannot be performed due to contamination of the sensor unit after the detection reaction.

[0005] To achieve the above-mentioned purpose and other related purposes, the present invention provides a microfluidic detection card capable of storage and sequential collection, wherein the microfluidic detection card is stacked in order from bottom to top: a base layer, a sensor layer, and a microfluidic chip layer;

[0006] The sensing layer comprises a sensing unit;

[0007] A plurality of detection cavities are formed between the sensing layer and the microfluidic chip layer; the sensing unit is integrated in each of the detection cavities and is suitable for connecting to a detection serial port;

[0008] The microfluidic chip layer is connected to a central liquid storage cavity, the microfluidic detection card is provided with an inlet and an outlet connected to the central liquid storage cavity, and a microchannel connected to the inlet, the central liquid storage cavity and the outlet in sequence is also provided between the sensing layer and the microfluidic chip layer. In the flow direction from the inlet to the outlet, the microchannel forms a positive guide Tesla valve and a negative guide Tesla valve in sequence, the intersection of the positive guide Tesla valve and the negative guide Tesla valve is connected to the central liquid storage cavity, and the detection cavity is connected in series to each branch flow channel of the positive guide Tesla valve and the negative guide Tesla valve.

[0009] In some embodiments of the present invention, the microfluidic chip layer is further provided with a semi-permeable membrane chamber adapted to communicate with the central liquid storage chamber.

[0010] In some embodiments of the present invention, the microfluidic chip layer is provided with a slot structure, and the central liquid storage cavity is detachably connected to the microfluidic chip layer through the slot structure.

[0011] In some embodiments of the present invention, the sensing unit is selected from an electrical sensing unit or an electrochemical sensing unit.

[0012] In some embodiments of the present invention, the inlet is arranged on the microfluidic chip layer; or, the inlet is arranged on the base layer, and a first through hole connected to the inlet is arranged at a position corresponding to the inlet on the sensing layer, so as to be suitable for sampling from the through hole of the base layer.

[0013] In some embodiments of the present invention, the outlet is arranged on the microfluidic chip layer; or, the outlet is arranged on the base layer, and a second through hole connected to the outlet is arranged at a position corresponding to the outlet on the sensing layer, so as to be suitable for sample discharge from the through hole of the base layer.

[0014] In some embodiments of the present invention, the base layer is a hard base material or a flexible base material.

[0015] As described above, the microfluidic detection card capable of storage and sequential collection of the present invention has the following beneficial effects:

[0016] The present invention's storable and time-sequentially collected microfluidic detection card combines microfluidic technology with sensor detection technology, designs an improved Tesla valve structure, and can realize trace liquid detection at different times, thereby analyzing the data changes of trace liquids. The present invention makes structural improvements based on the working principle of the Tesla valve, designs different numbers of trace liquid storage and detection unit structures, and embeds moisturizing crystals that reduce the evaporation of trace liquids. This design can not only reduce the interference of trace liquid gravity on the flow direction of trace liquids, reduce trace liquid reflux crosstalk, but also can achieve the purpose of time-sharing detection through the flow obstruction characteristics of the reverse-guiding Tesla valve, thereby obtaining more biological information. The detection card can be used for trace liquids, including but not limited to the detection of culture fluids, body fluids, and tissue secretions.

[0017] The microfluidic detection card capable of storing and sequentially collecting data of the present invention has a simple and reasonable structure, can prolong the storage time of trace liquids and reduce evaporation; a plurality of detection cavities are designed on the improved Tesla valve structure, and the flow obstruction characteristics of the reverse-guide Tesla valve can be utilized to separate trace liquids refluxed at different times, and the trace liquid data at different times can be detected and compared and analyzed, thereby reducing detection errors caused by individual differences. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The exploded view of the microfluidic detection card capable of storing and sequentially collecting data according to the present invention.

[0019] Figure 2 Schematic diagram of the storage and sequential collection unit in the microfluidic detection card of the present invention, the arrow direction is the liquid flow direction, the left side is the inlet, and the right side is the outlet.

[0020] Figure 3 Schematic diagram of positive and negative fluids in the Tesla valve structure in the microfluidic detection card of the present invention, a) forward flow; b) reverse flow.

[0021] Figure 4 Schematic diagram of flow simulation of filling trace amounts of liquid in the sequential detection chamber of the microfluidic detection card of the present invention at different times, a) T0; b) T0+10min; c) T0+40min; d) T0+80min; e) T0+120min.

[0022] Description of Figure Numbers

[0023] 100 Base layer

[0024] 200 Sensing layer

[0025] 300 Microfluidic Chip Layer

[0026] 400 Detect serial port

[0027] 11 First through hole

[0028] 21 Sensor unit

[0029] 31 Entrance

[0030] 32 Central reservoir

[0031] 33 Detection chamber

[0032] 34 Microfluidics

[0033] 35 Exit

[0034] 36 Semipermeable membrane chamber

[0035] 3A Positive Guide Tesla Valve

[0036] 3B Anti-Guide Tesla Valve DETAILED DESCRIPTION

[0037] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0038] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] In the present invention, the terms "first" and "second" are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. The orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0040] In addition, it should be understood that the combined connection relationship between one or more devices / apparatuses mentioned in the present invention does not exclude that other devices / apparatuses may exist before and after the combined device / apparatus or other devices / apparatuses may be inserted between the two devices / apparatuses explicitly mentioned, unless otherwise specified. It should be noted that the illustrations provided in this embodiment are only schematic illustrations of the basic concept of the present invention, and the drawings only show the components related to the present invention rather than the number, shape and size of the components in actual implementation. The type, quantity and proportion of each component in actual implementation may be changed arbitrarily, and the component layout type may also be more complicated.

[0041] In the description of the present invention, “several” means two or more than two, unless otherwise clearly and specifically defined.

[0042] The present invention provides a microfluidic detection card capable of storage and sequential collection, wherein the microfluidic detection card is stacked in order from bottom to top: a base layer 100, a sensor layer 200, and a microfluidic chip layer 300;

[0043] The sensing layer 200 includes a sensing unit 21;

[0044] A plurality of detection cavities 33 are formed between the sensing layer 200 and the microfluidic chip layer 300; the sensing unit 21 is integrated in each of the detection cavities 33 and is suitable for connecting to the detection serial port 400;

[0045] The microfluidic chip layer 300 is connected to a central liquid storage cavity 32, and the microfluidic detection card is also provided with an inlet 31 and an outlet 35 connected to the central liquid storage cavity 32. A microchannel 34 is formed between the sensing layer 200 and the microfluidic chip layer 300, which is connected to the inlet 31, the central liquid storage cavity 32 and the outlet 35 in sequence. In the flow direction from the inlet 31 to the outlet 35, the microchannel 34 forms a positive guide Tesla valve 3A and a negative guide Tesla valve 3B in sequence. The intersection of the positive guide Tesla valve 3A and the negative guide Tesla valve 3B is connected to the central liquid storage cavity 32, and the detection cavity 33 is connected in series to each branch flow channel of the positive guide Tesla valve 3A and the negative guide Tesla valve 3B.

[0046] In the present invention, the Tesla valve generally includes a main flow channel and a branch flow channel, wherein the main flow channel is the flow channel with the shortest distance from the inlet 31 to the outlet 35, and the branch flow channel is the flow channel connecting the main flow channel and the detection chamber 33. A positive-guided Tesla valve refers to a Tesla valve whose branch flow channel is directed toward the inlet 31, and a negative-guided Tesla valve refers to a Tesla valve whose branch flow channel is directed toward the outlet 35.

[0047] The storable and sequentially collected microfluidic detection card of the present invention provides a microfluidic detection structure that can detect trace amounts of liquid at different time intervals. First, the trace amount of liquid enters the central liquid storage chamber 32 smoothly along the main flow channel of the positively guided Tesla valve 3A. When the central liquid storage chamber 32 is filled with liquid, it slowly flows to the Tesla valve main flow channels on both sides of the central liquid storage chamber 32, and reaches the detection chamber 38 via the branch flow channels, and sequential detection is achieved through the integrated electrochemical sensing unit.

[0048] Among them, the Tesla valve involved is a passive one-way valve, which has little effect on the positive fluid, and the pressure on the negative fluid is hundreds of times that of the positive fluid.

[0049] The electrochemical sensing unit continuously monitors at a certain frequency according to demand. Due to the reverse characteristics of the improved Tesla valve, the reverse-flowing liquid will be subject to a certain pressure and flow slowly; when the liquid flows into each detection chamber, the component to be detected in the liquid will combine with the modified sensing unit to generate a signal. In the process of continuous monitoring at a certain frequency, the Tesla valve in the present invention can extend the interval time of the electrochemical reaction in adjacent detection chambers, thereby realizing the detection of liquid components at different times. Compared with the current detection card, more liquid composition information can be obtained. The forward flow mentioned in the present invention refers to the following. Figure 3 a shows the flow direction, and the reverse flow mentioned refers to Figure 3 b shows the flow direction, the same below.

[0050] The multiple detection chambers in series with the Tesla valve in the present invention can effectively prevent the reverse flow of liquid through the design of structure and length. On the one hand, when the liquid flows forward into the forward-guiding Tesla valve, it will not be disturbed by the detection chamber; on the other hand, the speed of the liquid flowing backward in the forward-guiding Tesla valve or forward in the reverse-guiding Tesla valve is slow, realizing the retention storage and detection of trace liquids in different time sequences.

[0051] The present invention can design and increase the number and structural dimensions of Tesla valve units according to actual detection requirements to adjust the detection time and frequency. Therefore, the present invention can achieve time-series collection and instant detection. Since the central liquid storage chamber 32 is detachable, it can also meet the offline detection requirements of trace liquids.

[0052] In the microfluidic detection card of the present invention, the microfluidic chip layer 300 is further provided with a semi-permeable membrane chamber 36 suitable for communicating with the central liquid storage chamber 32 .

[0053] The semipermeable membrane chamber 36 is formed by a semipermeable membrane material wrapping a moisturizing crystal.

[0054] The semipermeable membrane material must meet the following conditions: allowing water molecules to pass through; and limiting the passage of macromolecules. In a preferred embodiment of the present invention, the semipermeable membrane material is a polymerized fullerene semipermeable membrane.

[0055] The moisturizing crystal is a carrier loaded with crystal water or hydrated water. The carrier is preferably but not limited to one or more of mica, kaolinite, and zeolite. The moisturizing crystal is prepared by hydration, solution evaporation, surface modification, or freeze drying.

[0056] The use of polymer materials or nanomaterials to wrap the moisturizing crystals prevents them from direct contact with liquids without affecting the diffusion of water molecules, so that high humidity is maintained in the device and liquid evaporation is reduced. At the same time, the permeability of the semipermeable membrane material to small molecules allows water molecules to pass smoothly; and prevents trace amounts of liquid from contacting the water-containing crystals and causing contamination.

[0057] In the microfluidic detection card of the present invention, the microfluidic chip layer 300 is provided with a card slot structure, and the central liquid storage chamber 32 is detachably connected to the microfluidic chip layer 300 through the card slot structure. The central liquid storage chamber 32 is detachable and replaceable. The central liquid storage chamber 32 is sealed and connected to the main flow channel of the Tesla valve on both sides so that the fluid can flow smoothly between the Tesla valve and the central liquid storage chamber. Conventional technology can be used to achieve the sealed connection, for example, a sealing material such as a sealing ring is used at the junction of the two.

[0058] In the microfluidic detection card of the present invention, the electrochemical sensor unit 21 is selected from an electrical sensor unit or an electrochemical sensor unit.

[0059] In the microfluidic detection card of the present invention, the inlet 31 is arranged on the microfluidic chip layer 300; or, the inlet 31 is arranged on the base layer 100, and a first through hole 11 connected to the inlet 31 is arranged at a position corresponding to the inlet 31 on the sensing layer 200, so as to be suitable for sample injection from the base layer 100.

[0060] In the microfluidic detection card of the present invention, the outlet 35 is arranged on the microfluidic chip layer 300; or, the outlet 35 is arranged on the base layer 100, and a second through hole connected to the outlet 35 is arranged at a position corresponding to the outlet 35 on the sensing layer 200, so as to be suitable for sample discharge from the base layer 100.

[0061] The base layer 100 is a hard base material or a flexible base material.

[0062] The hard base material is selected from a silicon wafer and a glass wafer.

[0063] The flexible substrate material is selected from one of polyethylene terephthalate, polyimide and polystyrene.

[0064] Example 1

[0065] Microfluidic detection card that can store and collect data in a timely manner:

[0066] like Figure 1 As shown, the following are stacked from bottom to top: a base layer 100, a sensing layer 200, and a microfluidic chip layer 300; the sensing layer 200 includes a sensing unit 21, and a plurality of detection cavities 33 are formed between the sensing layer 200 and the microfluidic chip layer 300; the electrochemical sensing unit 21 specifically includes a reference electrode, a counter electrode, and a detection electrode integrated in the detection cavity 33, and connected to a detection serial port 400; the microfluidic chip layer 300 is provided with a card slot structure, and the central liquid storage cavity 32 is connected to the microfluidic chip layer 300 through the card slot structure, and the microfluidic detection card is provided with a connecting central liquid storage cavity 32. The inlet 31 and the outlet 35 of the liquid storage chamber 32, and a microchannel 34 that sequentially connects the inlet 31, the central liquid storage chamber 32 and the outlet 35 are formed between the sensing layer 200 and the microfluidic chip layer 300. In the flow direction from the inlet 31 to the outlet 35, the microchannel 34 sequentially forms a positive guide Tesla valve 3A and a negative guide Tesla valve 3B. The intersection of the positive guide Tesla valve 3A and the negative guide Tesla valve 3B is connected to the central liquid storage chamber 32, and the detection chamber 33 is connected in series to each branch flow channel of the positive guide Tesla valve 3A and the negative guide Tesla valve 3B.

[0067] In this device, if Figure 2 As shown, the positive guide Tesla valve 3A is connected to three detection chambers, namely 33-1, 33-2 and 33-3, and the negative guide Tesla valve 3B is connected to three detection chambers, namely 33-1', 33-2' and 33-3'. The positive guide Tesla valve 3A and the detection chamber 33 connected thereto are symmetrically arranged with the negative guide Tesla valve 3B and the detection chamber connected thereto; in the device, the inlet 31 is arranged on the base layer 100, and the position corresponding to the inlet 31 on the sensing layer 200 is provided with a first through hole 11 connected to the inlet 31, so as to be suitable for sampling from the base layer 100; the microfluidic chip layer 300 is also provided with a semipermeable membrane chamber 36 suitable for communicating with the central liquid storage chamber 32, and the semipermeable membrane chamber 36 is formed by polymerized fullerene semipermeable membrane wrapped with quartz. The arrow direction is the liquid flow direction, the left side is the inlet, and the right side is the outlet.

[0068] like Figure 3 As shown in Figure 2, when the fluid flows from left to right, that is, forward flow, the fluid motion state is as follows: Figure 3 a. Due to the characteristics of the Tesla valve, the forward fluid is not affected and preferentially flows into the central liquid storage chamber 32. The added liquid storage chamber has little effect on the forward flowing liquid, and a small amount of liquid enters the central liquid storage chamber. When the central liquid storage chamber 32 is filled with a small amount of liquid, the liquid will flow to the Tesla valve structures on both sides. The flow direction in the Tesla valve is as follows: Figure 3As shown in b; due to the resistance of the Tesla valve to the reverse flow of liquid, the liquid is retained by the special structure of the Tesla valve, and the trace liquid will flow into the three symmetrical sequential detection chambers 33-1 and 33-1', 33-2 and 33-2', 33-3 and 33-3' in sequence, extending the time for the liquid to fill the Tesla valve. Then the detection electrodes in each sequential detection chamber realize the detection of the target object in the trace liquid at different times through electrochemical reaction, and realize the digital-to-analog conversion and analysis of the analyte through the detection serial port 400.

[0069] like Figure 4 As shown in the figure, it is a flow simulation diagram of the microfluidic detection chamber in the microfluidic detection card filled with trace amounts of liquid at different times, where the white area represents sweat. Figure 4 As shown in b, at 10 minutes, the trace liquid first fills the central liquid storage chamber 32, and then passes through the microchannel 34 to fill the sequential detection chambers on both sides of the central liquid storage chamber 32 ( Figure 4 c, 4d, 4e): at 40 min, the detection chambers 33-1 and 33-1' are filled; at 80 min, the detection chambers 33-2 and 33-2' are filled; at 120 min, the detection chambers 33-3 and 33-3' are filled.

[0070] It can be seen that the microfluidic detection card capable of storage and sequential collection of the present invention can control the flow rate by designing the structure, length and width of the Tesla valve, so as to achieve the time difference of the trace liquid filling different sequential detection chambers, thereby realizing the component detection and comparative analysis of the trace liquid at different times. Furthermore, by designing the volume of the sequential detection chamber, the volume required for the trace liquid to flow back to the inlet is increased, and the interval time of the trace liquid entering the sequential detection chamber is extended.

[0071] In summary, the microfluidic detection card of the present invention that can store and collect in a timely manner can detect target analytes in trace liquids at different times, is suitable for long-term and short-term trace liquid collection and detection, and reduces the reflux and volatilization of trace liquids to a certain extent; at the same time, different numbers of Tesla valve units can be designed according to needs, showing extremely high flexibility; the detection card can be used for the detection of trace cell culture fluids, sweat, tissue secretions, etc. Therefore, the present invention effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0072] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A microfluidic detection card capable of storing and sequentially collecting data, characterized in that: The microfluidic detection card is stacked in order from bottom to top: a base layer (100), a sensor layer (200), and a microfluidic chip layer (300); The sensing layer (200) comprises a sensing unit (21); A plurality of detection cavities (33) are formed between the sensing layer (200) and the microfluidic chip layer (300); the sensing unit (21) is integrated in each of the detection cavities (33) and is suitable for connecting to a detection serial port (400); The microfluidic chip layer (300) is connected to a central liquid storage cavity (32), and the microfluidic detection card is also provided with an inlet (31) and an outlet (35) connected to the central liquid storage cavity (32). A microfluidic channel (34) connected to the inlet (31), the central liquid storage cavity (32) and the outlet (35) is formed between the sensing layer (200) and the microfluidic chip layer (300). In the flow direction from the inlet (31) to the outlet (35), the microfluidic channel (34) forms a positive-guided Tesla valve (3A) and a negative-guided Tesla valve (3B) in sequence. The intersection of the positive-guided Tesla valve (3A) and the negative-guided Tesla valve (3B) is connected to the central liquid storage cavity (32), and the detection cavity (33) is connected in series to each branch flow channel of the positive-guided Tesla valve (3A) and the negative-guided Tesla valve (3B).

2. The microfluidic detection card capable of storage and sequential collection according to claim 1, characterized in that: The microfluidic chip layer (300) is also provided with a semi-permeable membrane chamber (36) suitable for communicating with the central liquid storage chamber (32).

3. The microfluidic detection card capable of storage and sequential collection according to claim 2, characterized in that: The semipermeable membrane chamber (36) is formed by wrapping a moisturizing crystal with a semipermeable membrane material.

4. The microfluidic detection card capable of storage and sequential collection according to claim 3, characterized in that: The semipermeable membrane material is a polymerized fullerene semipermeable membrane.

5. The microfluidic detection card capable of storage and sequential collection according to claim 3, characterized in that: The moisturizing crystals are one or more of mica, kaolinite and zeolite loaded with crystal water or hydrated water.

6. The microfluidic detection card capable of storage and sequential collection according to claim 1, characterized in that: The microfluidic chip layer (300) is provided with a slot structure, and the central liquid storage cavity (32) is detachably connected to the microfluidic chip layer (300) via the slot structure.

7. The microfluidic detection card capable of storage and sequential collection according to claim 1, characterized in that: The sensing unit (21) is selected from an electrical sensing unit or an electrochemical sensing unit.

8. The microfluidic detection card capable of storage and sequential collection according to claim 1, characterized in that: The inlet (31) is arranged on the microfluidic chip layer (300); or, the inlet (31) is arranged on the base layer (100), and a first through hole (11) communicating with the inlet (31) is arranged at a position corresponding to the inlet (31) on the sensing layer (200), so as to be suitable for injecting samples from the base layer (100); And / or, the outlet (35) is arranged on the microfluidic chip layer (300); or, the outlet (35) is arranged on the base layer (100), and a second through hole connected to the outlet (35) is arranged at a position corresponding to the outlet (35) on the sensing layer (200), so as to be suitable for taking out samples from the base layer (100).

9. The microfluidic detection card capable of storage and sequential collection according to claim 1, characterized in that: The base layer (100) is a hard base material or a flexible base material.

10. The microfluidic detection card capable of storage and sequential collection according to claim 9, characterized in that: The hard substrate material is selected from one of a silicon wafer and a glass wafer; And / or, the flexible substrate material is selected from one of polyethylene terephthalate, polyimide and polystyrene.

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