Method for improving heart failure after myocardial infarction through cold air inhalation

Abstract

The invention relates to the technical field of myocardial infarction treatment, and particularly discloses a method for improving heart failure after myocardial infarction by cold air inhalation, which comprises the following steps: step 1, selecting a plurality of mice for experiments, and placing the mice for experiments in an experiment area; 2, preparing air for the laboratory mouse to inhale; 3, sucking the prepared air into the experimental mouse; and 4, observing the mouse which inhales the air and is used for the experiment. Air inhalation at a proper temperature is given to the myocardial infarction mouse through the air inhalation device, so that the lifetime of the myocardial infarction mouse can be obviously prolonged, the cardiac function and the myocardial infarction area of the myocardial infarction mouse can be improved, and the cardiac sympathetic nerve activity of the myocardial infarction mouse can be reduced; air inhalation at a proper temperature can be used as a new means for improving prognosis of patients with myocardial infarction, and a new thought and method can be provided for treating or preventing heart failure after myocardial infarction.

Description

Technical Field

[0001] The invention belongs to the technical field of myocardial infarction treatment, and specifically relates to a method for improving heart failure after myocardial infarction by inhaling cold air. Background Art

[0002] Myocardial infarction (MI) is one of the most common cardiovascular diseases that causes heart failure (HF) and clinical death. Myocardial infarction has a poor prognosis and a high mortality rate. Although important progress has been made in reperfusion therapy technologies such as percutaneous coronary intervention and thrombolysis, 20% to 30% of myocardial infarction patients still develop HF within one year of discharge, and their mortality rate is 5 times that of patients without HF. Cardiac pathological remodeling is the pathological basis for the development of HF in patients with myocardial infarction. In-depth analysis of the mechanism of cardiac pathological remodeling after myocardial infarction and finding effective prevention and control measures are one of the current hot spots and difficulties in the field of cardiology.

[0003] Studies have shown that ambient temperature is closely related to the occurrence, development and prognosis of a variety of cardiovascular diseases. A Chinese study involving 5,303 patients with acute coronary syndrome in emergency hospitalization showed that compared with normal room temperature (25°C), the cumulative relative risk of acute coronary syndrome when exposed to excessively low temperatures (-1.6°C) was 2.14, and the cumulative relative risk of acute coronary syndrome when exposed to excessively high temperatures (31.4°C) was 1.66, indicating that both low and high temperatures can significantly increase the risk of acute coronary syndrome, affecting more than 30 million cardiovascular deaths in 567 cities in 27 countries around the world. An analysis of cases revealed that the risk of death from ischemic heart disease showed a J-shaped curve relationship with the ambient temperature. Among every 1,000 cardiovascular deaths, 2 and 9 deaths were attributed to extremely high and extremely low temperatures, respectively. The above studies suggest that excessive cold and excessive heat can cause cardiovascular disease and worsen the prognosis. However, in the prior art, there has been no experimental research to prove the role of intervening in ambient temperature in the prognosis of myocardial infarction in improving heart failure after myocardial infarction. In view of the above situation, in order to overcome the shortcomings of existing clinical treatments, the present invention provides a method of improving heart failure after myocardial infarction by inhaling cold air.

[0004] The present invention provides myocardial infarction mice with air inhalation at an appropriate temperature through an air inhalation device, which can significantly prolong the survival period of the myocardial infarction mice, improve their cardiac function and myocardial infarction area, and reduce the cardiac sympathetic nerve activity of the myocardial infarction mice. Therefore, air inhalation at an appropriate temperature can be used as a new means to improve the prognosis of myocardial infarction patients, and can also provide new ideas and methods for treating or preventing heart failure after myocardial infarction. Summary of the invention

[0005] The purpose of the present invention is to provide a method for improving heart failure after myocardial infarction by inhaling cold air, so as to solve the problems in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method of cold air inhalation for improving heart failure after myocardial infarction, comprising:

[0008] Step 1: Select a plurality of mice for the experiment and place the mice in the experimental area;

[0009] Step 2: Prepare the air for inhalation by experimental mice;

[0010] Step 3: Let the experimental mice inhale the prepared air;

[0011] Step 4: Observe the mice that inhale air for the experiment.

[0012] It can be seen from the above that, through the operational settings of the above steps, the present invention can give myocardial infarction mice air inhalation of appropriate temperature through the air inhalation device, which can significantly prolong the survival time of myocardial infarction mice, improve their cardiac function and myocardial infarction area, and reduce the cardiac sympathetic nerve activity of myocardial infarction mice. Therefore, air inhalation of appropriate temperature can be used as a new means to improve the prognosis of patients with myocardial infarction, and can also provide new ideas and methods for treating or preventing heart failure after myocardial infarction.

[0013] Wherein, the mice used for the experiment are mice suffering from myocardial infarction, the prepared air is ordinary air, and the air does not contain any added drugs.

[0014] The prepared air temperature is 16°C±1°C, and the mice inhale air for 8 hours per day.

[0015] From the above, we can see that inhaling warm air has the following benefits for mice with myocardial infarction and heart failure;

[0016] By adjusting the indoor temperature and keeping it within an appropriate range, the cardiac burden of mice with myocardial infarction can be reduced. Appropriate temperature helps lower heart rate and blood pressure, keeping the cardiovascular system in a relatively stable state. Appropriate temperature can promote blood circulation, increase blood vessel elasticity, and help reduce the formation of blood clots, which is especially important for mice with myocardial infarction because blood clots are one of the risk factors for recurrence of myocardial infarction. A comfortable ambient temperature can improve the comfort of patients with myocardial infarction and reduce physical discomfort caused by cold or overheating.

[0017] Among them, the observation contents after the mice inhaled air were the myocardial contractile function of the mice, the myocardial infarction area of ​​the mice and the cardiac sympathetic nerve activity of the mice.

[0018] From the above, we can see that experimental observation of mouse myocardial contractile function, mouse myocardial infarction area and mouse cardiac sympathetic nerve activity has the following benefits:

[0019] The benefits of experimental observation of mouse myocardial contractile function are as follows:

[0020] By observing the contractile properties of mouse cardiomyocytes, we can gain a deeper understanding of how cardiomyocytes work in a coordinated manner to produce the heart's pumping function, which helps to reveal the molecular mechanisms and signaling pathways of cardiomyocytes during contraction; experimental observation of myocardial contractile function can serve as an important indicator for assessing heart health; by comparing the myocardial contractile function of normal mice and disease model mice, we can determine whether heart function is impaired and the extent of the damage; understanding myocardial contractile function will help develop treatment strategies for cardiovascular diseases.

[0021] The benefits of experimental observation of myocardial infarction area in mice are as follows:

[0022] Infarct size is an important indicator of the severity of myocardial infarction. By observing the infarct size of mice, the extent of myocardial infarction can be intuitively evaluated, providing an important basis for disease diagnosis and treatment. Experimental observation of infarct size helps to reveal the pathogenesis of myocardial infarction.

[0023] For example, by comparing the infarct size of different myocardial infarction model mice, the key factors in the occurrence and development of myocardial infarction can be explored;

[0024] By observing the effects of drugs on the area of ​​myocardial infarction in mice, drugs with the potential to treat myocardial infarction can be screened out. This provides an important means for the development of new drugs and helps accelerate the development of cardiovascular drugs.

[0025] The benefits of experimental observation of cardiac sympathetic nerve activity in mice are as follows:

[0026] Experimental observations of cardiac sympathetic nerve activity in mice help reveal the neural mechanisms of heart disease and provide new ideas for the diagnosis and treatment of the disease. By regulating cardiac sympathetic nerve activity, it may be possible to improve heart function and treat heart disease. Understanding cardiac sympathetic nerve activity in mice can help develop new cardiac neuroregulation therapies and provide more treatment options for patients with cardiovascular disease.

[0027] Wherein, the device for detecting heart function is a small animal ultrasonic imaging system.

[0028] From the above, we can see that the detection of cardiac function of small animals by ultrasound imaging system has the following benefits:

[0029] Ultrasound imaging is a non-invasive examination method that does not cause trauma to small animals, so cardiac function can be evaluated without disturbing their normal physiological state. Compared with imaging techniques such as X-rays, ultrasound imaging has no radiation hazards and is suitable for examination of small animals of all ages and for repeated examinations.

[0030] The ultrasound imaging system can display the structure and functional status of the heart of small animals in real time, and doctors can observe the dynamic changes of the heart at different time points. Through real-time dynamic observation, doctors can analyze various indicators of the heart during the cardiac cycle, such as ejection fraction, shortening rate of left ventricular internal diameter, etc., so as to more accurately evaluate heart function.

[0031] Modern ultrasound imaging systems have extremely high resolution and can clearly display the various structures and details of the heart of small animals. Through ultrasound imaging, doctors can accurately measure parameters such as the size and shape of the heart, as well as the size and volume of each chamber, providing important basis for the diagnosis of heart disease.

[0032] Wherein, the reagent for detecting the myocardial infarction area is a 2,3,5-triphenyltetrazolium chloride (TTC) staining kit.

[0033] From the above, we can see that the detection of myocardial infarction area in small animals using 2,3,5-triphenyltetrazolium chloride (TTC) staining kit has the following benefits:

[0034] The TTC staining kit uses the properties of TTC as a redox indicator to dye living cells red by reacting with dehydrogenases in living cells (especially succinate dehydrogenase in mitochondria). When cells die, dehydrogenases lose their activity and TTC cannot react with them, so the infarcted area appears white. This clear detection principle makes TTC staining an effective method for evaluating the area of ​​cardiac infarction. At the same time, TTC staining is easy to operate and does not require complicated equipment and steps, making it suitable for laboratory environments.

[0035] The heart sections after TTC staining can clearly show the boundary between the infarct area and the normal area. The infarct area appears white, while the normal area appears red. This obvious color contrast makes the observation results intuitive and easy to judge. In addition, TTC staining can also be used to evaluate the status of the infarct edge area, providing researchers with more comprehensive information on myocardial infarction.

[0036] The TTC staining method has good repeatability and accuracy. The experimental results show that the staining results between different batches are not much different and can accurately reflect the size of myocardial infarction. This stability and accuracy make TTC staining one of the standard methods for evaluating myocardial infarction area.

[0037] The TTC staining kit is not only suitable for small animal models such as mice, but can also be used to detect myocardial infarction area in other animals, which makes TTC staining have broad application prospects in cardiovascular disease research. At the same time, TTC staining can also be combined with other experimental methods (such as electrocardiogram, pathological examination, etc.) to provide researchers with more comprehensive experimental data.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] The present invention provides myocardial infarction mice with air inhalation at an appropriate temperature through an air inhalation device, which can significantly prolong the survival period of the myocardial infarction mice, improve their cardiac function and myocardial infarction area, and reduce the cardiac sympathetic nerve activity of the myocardial infarction mice. Therefore, air inhalation at an appropriate temperature can be used as a new means to improve the prognosis of myocardial infarction patients, and can also provide new ideas and methods for treating or preventing heart failure after myocardial infarction. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 The survival curves of the myocardial infarction mice of the present invention inhaling cold air and room temperature gas respectively;

[0041] Figure 2 These are ultrasound images of the hearts of two groups of mice with myocardial infarction;

[0042] Figure 3 The statistical graphs are for ejection fraction and short axis shortening rate of two groups of mice with myocardial infarction;

[0043] Figure 4 Representative images of TTC staining of hearts of two groups of mice with myocardial infarction;

[0044] Figure 5 This is a statistical diagram of the myocardial infarction area of ​​the two groups of mice with myocardial infarction;

[0045] Figure 6 This is the ratio of cardiac low frequency power (LF) to high frequency power (HF) of the three groups of mice at one and three weeks of gas inhalation. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 As shown, a method of cold air inhalation for improving heart failure after myocardial infarction comprises:

[0048] Step 1: Select a plurality of mice for the experiment and place the mice in the experimental area;

[0049] Step 2: Prepare the air for inhalation by experimental mice;

[0050] Step 3: Let the experimental mice inhale the prepared air;

[0051] Step 4: Observe the mice that inhale air for the experiment.

[0052] It can be seen from the above that, through the operational settings of the above steps, the present invention can give myocardial infarction mice air inhalation of appropriate temperature through the air inhalation device, which can significantly prolong the survival time of myocardial infarction mice, improve their cardiac function and myocardial infarction area, and reduce the cardiac sympathetic nerve activity of myocardial infarction mice. Therefore, air inhalation of appropriate temperature can be used as a new means to improve the prognosis of patients with myocardial infarction, and can also provide new ideas and methods for treating or preventing heart failure after myocardial infarction.

[0053] Wherein, the mice used for the experiment are mice suffering from myocardial infarction, the prepared air is ordinary air, and the air does not contain any added drugs.

[0054] The prepared air temperature is 16°C±1°C, and the mice inhale air for 8 hours per day.

[0055] From the above, we can see that inhaling warm air has the following benefits for mice with myocardial infarction and heart failure;

[0056] By adjusting the indoor temperature and keeping it within an appropriate range, the cardiac burden of mice with myocardial infarction can be reduced. Appropriate temperature helps lower heart rate and blood pressure, keeping the cardiovascular system in a relatively stable state. Appropriate temperature can promote blood circulation, increase blood vessel elasticity, and help reduce the formation of blood clots, which is especially important for mice with myocardial infarction because blood clots are one of the risk factors for recurrence of myocardial infarction. A comfortable ambient temperature can improve the comfort of patients with myocardial infarction and reduce physical discomfort caused by cold or overheating.

[0057] Among them, the observation contents after the mice inhaled air were the myocardial contractile function of the mice, the myocardial infarction area of ​​the mice and the cardiac sympathetic nerve activity of the mice.

[0058] From the above, we can see that experimental observation of mouse myocardial contractile function, mouse myocardial infarction area and mouse cardiac sympathetic nerve activity has the following benefits:

[0059] The benefits of experimental observation of mouse myocardial contractile function are as follows:

[0060] By observing the contractile properties of mouse cardiomyocytes, we can gain a deeper understanding of how cardiomyocytes work in a coordinated manner to produce the heart's pumping function, which helps to reveal the molecular mechanisms and signaling pathways of cardiomyocytes during contraction; experimental observation of myocardial contractile function can serve as an important indicator for assessing heart health; by comparing the myocardial contractile function of normal mice and disease model mice, we can determine whether heart function is impaired and the extent of the damage; understanding myocardial contractile function will help develop treatment strategies for cardiovascular diseases.

[0061] The benefits of experimental observation of myocardial infarction area in mice are as follows:

[0062] Infarct size is an important indicator of the severity of myocardial infarction. By observing the infarct size of mice, the extent of myocardial infarction can be intuitively evaluated, providing an important basis for disease diagnosis and treatment. Experimental observation of infarct size helps to reveal the pathogenesis of myocardial infarction.

[0063] For example, by comparing the infarct size of different myocardial infarction model mice, the key factors in the occurrence and development of myocardial infarction can be explored;

[0064] By observing the effects of drugs on the area of ​​myocardial infarction in mice, drugs with the potential to treat myocardial infarction can be screened out. This provides an important means for the development of new drugs and helps accelerate the development of cardiovascular drugs.

[0065] The benefits of experimental observation of cardiac sympathetic nerve activity in mice are as follows:

[0066] Experimental observations of cardiac sympathetic nerve activity in mice help reveal the neural mechanisms of heart disease and provide new ideas for the diagnosis and treatment of the disease. By regulating cardiac sympathetic nerve activity, it may be possible to improve heart function and treat heart disease. Understanding cardiac sympathetic nerve activity in mice can help develop new cardiac neuroregulation therapies and provide more treatment options for patients with cardiovascular disease.

[0067] Wherein, the device for detecting heart function is a small animal ultrasonic imaging system.

[0068] From the above, we can see that the detection of cardiac function of small animals by ultrasound imaging system has the following benefits:

[0069] Ultrasound imaging is a non-invasive examination method that does not cause trauma to small animals, so cardiac function can be evaluated without disturbing their normal physiological state. Compared with imaging techniques such as X-rays, ultrasound imaging has no radiation hazards and is suitable for examination of small animals of all ages and for repeated examinations.

[0070] The ultrasound imaging system can display the structure and functional status of the heart of small animals in real time, and doctors can observe the dynamic changes of the heart at different time points. Through real-time dynamic observation, doctors can analyze various indicators of the heart during the cardiac cycle, such as ejection fraction, shortening rate of left ventricular internal diameter, etc., so as to more accurately evaluate heart function.

[0071] Modern ultrasound imaging systems have extremely high resolution and can clearly display the various structures and details of the heart of small animals. Through ultrasound imaging, doctors can accurately measure parameters such as the size and shape of the heart, as well as the size and volume of each chamber, providing important basis for the diagnosis of heart disease.

[0072] Wherein, the reagent for detecting the myocardial infarction area is a 2,3,5-triphenyltetrazolium chloride (TTC) staining kit.

[0073] From the above, we can see that the detection of myocardial infarction area in small animals using 2,3,5-triphenyltetrazolium chloride (TTC) staining kit has the following benefits:

[0074] The TTC staining kit uses the properties of TTC as a redox indicator to dye living cells red by reacting with dehydrogenases in living cells (especially succinate dehydrogenase in mitochondria). When cells die, dehydrogenases lose their activity and TTC cannot react with them, so the infarcted area appears white. This clear detection principle makes TTC staining an effective method for evaluating the area of ​​cardiac infarction. At the same time, TTC staining is easy to operate and does not require complicated equipment and steps, making it suitable for laboratory environments.

[0075] The heart sections after TTC staining can clearly show the boundary between the infarct area and the normal area. The infarct area appears white, while the normal area appears red. This obvious color contrast makes the observation results intuitive and easy to judge. In addition, TTC staining can also be used to evaluate the status of the infarct edge area, providing researchers with more comprehensive information on myocardial infarction.

[0076] The TTC staining method has good repeatability and accuracy. The experimental results show that the staining results between different batches are not much different and can accurately reflect the size of myocardial infarction. This stability and accuracy make TTC staining one of the standard methods for evaluating myocardial infarction area.

[0077] The TTC staining kit is not only suitable for small animal models such as mice, but can also be used to detect myocardial infarction area in other animals, which makes TTC staining have broad application prospects in cardiovascular disease research. At the same time, TTC staining can also be combined with other experimental methods (such as electrocardiogram, pathological examination, etc.) to provide researchers with more comprehensive experimental data.

[0078] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for improving heart failure after myocardial infarction by inhaling cold air, characterized in that: include: Step 1: Select a plurality of mice for the experiment and place the mice in the experimental area; Step 2: Prepare the air for inhalation by experimental mice; Step 3: Let the experimental mice inhale the prepared air; Step 4: Observe the mice that inhale air for the experiment.

2. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The mice used in the experiment were mice with myocardial infarction.

3. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The prepared air is ordinary air, and the air does not contain any added medicine.

4. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The prepared air temperature is 16°C±1°C.

5. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The mice inhaled air for 8 hours per day.

6. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The observation contents after the mice inhaled air included the myocardial contractile function of the mice, the myocardial infarction area of ​​the mice and the cardiac sympathetic nerve activity of the mice.

7. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The air was injected into the interior of the mouse through a gas inhalation device.

8. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 1, characterized in that: The device for detecting cardiac function is a small animal ultrasonic imaging system.

9. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 6, characterized in that: The cardiac sympathetic nerve activity of the mice was detected by a small animal physiological recorder MP160.

10. The method of improving heart failure after myocardial infarction by cold air inhalation according to claim 9, characterized in that: The reagent for detecting the cardiac infarction area is a 2,3,5-triphenyltetrazolium chloride (TTC) staining kit.

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