Animal model construction method for wearing invisible appliance in mouth of mouse

Abstract

The invention discloses an animal model construction method for wearing an invisible appliance in the mouth of a mouse, and belongs to the field of oral cavity models. The method for constructing the animal model with the mouse wearing the invisible orthodontic appliance in the mouth comprises the steps that after the invisible orthodontic appliance is manufactured, the cheek-tongue side, opposite to the adjacent gap between a first molar and a second molar, of the invisible orthodontic appliance is punched, the incisor part on the invisible orthodontic appliance is ground away, and only the root is reserved; and during wearing, a ligature wire penetrates through a pre-hole part on the cheek-tongue side of the invisible appliance, and ligature is carried out. On the premise that the surface structure and color of teeth are not affected, clinical effective experimental data can be obtained, the invisible orthodontic appliance adopts rat incisor partial windowing, and the problem that the invisible orthodontic appliance cannot be worn after rat incisor growth is solved.

Description

Technical Field

[0001] The invention relates to the field of oral models, and in particular to a method for constructing an animal model in which a mouse wears an invisible orthodontic appliance. Background Art

[0002] In recent years, bracketless invisible braces have been favored by orthodontists and patients for their beauty, comfort, and removability. The number of patients has increased explosively. However, studies have shown that wearing invisible braces will increase plaque accumulation and interfere with oral hygiene, and may also cause some adverse reactions, such as gingivitis, periodontitis, and leukoplakia.

[0003] The appearance of gingivitis, periodontitis and white spot lesions caused by wearing invisible braces not only violates the aesthetic principles of orthodontic treatment, but also damages oral health. It is an urgent problem to be solved in the process of invisible braces without brackets. There are many factors that affect the oral hygiene of patients wearing invisible braces, and the influencing mechanisms are also different. Some implementation measures cannot be studied directly on humans, so animal experiments have become one of the basic experimental methods of invisible braces.

[0004] The results of animal experiments depend on a well-established animal experimental model, and the establishment of this model first depends on the selection of appropriate experimental animals. There are many methods to choose from when creating an animal model for wearing invisible braces. As a common rodent, rats have the advantages of a short growth cycle, easy to raise, low price, and suitable for large sample observations. Therefore, they are often selected as experimental animals for wearing invisible braces.

[0005] In the model of rats wearing invisible braces, it is necessary to ensure that the invisible braces do not fall off in the rats' mouths, but practice has shown that it is difficult to ensure that the invisible braces do not fall off in the rats' mouths and do not affect the tooth surface. Some studies have used light-curing resin to bond invisible braces to rat teeth, but due to the growth of rat incisors, the invisible braces need to be replaced once a week, and the bonding method will damage the tooth surface. In addition, the residue of the adhesive will make it difficult to judge the degree of demineralization and color change on the surface of the rat teeth. In addition, the use of light-curing resin requires complete isolation of saliva for successful bonding, but the operating space in the rat's mouth is small, and it is very difficult to completely isolate the saliva. Summary of the invention

[0006] The purpose of the present invention is to provide a method for constructing an animal model of wearing an invisible brace in the mouth of a rat to solve the problems existing in the above-mentioned prior art. The present invention can obtain clinically effective experimental data without affecting the surface structure and color of the teeth. The invisible brace adopts a window in the incisor part of the rat to solve the problem that the invisible brace cannot be worn after the incisor part of the rat grows.

[0007] To achieve the above object, the present invention provides the following solutions:

[0008] The present invention provides a method for constructing an animal model of wearing an invisible brace in the mouth of a mouse, comprising the steps of making a hole on the buccal and lingual sides of the invisible brace opposite to the interproximal space between a first molar and a second molar, grinding off the incisor part of the invisible brace to retain only the root; and passing a ligature wire through the pre-hole part on the buccal and lingual sides of the invisible brace when wearing the invisible brace, and ligating the hole.

[0009] Furthermore, before passing the ligature wire through the pre-hole site on the buccal and lingual sides of the invisible braces, the method also includes passing an orthodontic ligature wire with a larger diameter through the interproximal space between the first molar and the second molar of the rat.

[0010] Furthermore, the ligature wire passing through the pre-holes on the buccal and lingual sides of the invisible braces is an orthodontic ligature wire with a diameter of 0.025 mm.

[0011] Furthermore, the orthodontic ligature wire with a larger diameter is an orthodontic ligature wire with a diameter of 0.030 mm.

[0012] Furthermore, the production of invisible braces also includes the steps of making individual trays, taking molds and pouring molds.

[0013] Furthermore, compared with the mouse skull, the size of the individual tray is 3 mm larger than the front end of the incisor, 3 mm larger than the rear end of the molar, and 5 mm larger than the range of the impression on both sides.

[0014] Furthermore, after ligation, the method further comprises the step of coating the ligation wire tail with a fluid resin and solidifying it.

[0015] The present invention discloses the following technical effects:

[0016] The animal model for wearing invisible braces in the mouth of rats provided by the present invention not only overcomes the problem in traditional experimental methods that invisible braces cannot be worn for a long time after the rat incisors have partially grown, and the physical damage to the rat teeth caused by the wearing process, but also successfully maintains the original natural structure and color of the teeth, providing researchers with a new, non-invasive research method. The animal model constructed by the method of the present invention is conducive to obtaining more accurate data with clinical translation potential, which greatly promotes the progress of animal experimental research in the field of oral orthodontics. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 This is a diagram of a rat wearing an invisible brace in its mouth. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0021] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0022] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0023] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0024] Example

[0025] A method for constructing an animal model of a mouse wearing an invisible brace appliance in the mouth comprises the following steps:

[0026] S1. Make individual pallets

[0027] Photosensitive resin was used to make individual trays for rats based on the rat skull after dissection, so that the individual trays for rats were slightly larger than the front end of the incisor by 3mm, the rear end of the molar by 3mm, and the range of the molding on both sides by 5mm.

[0028] S2. Modulus

[0029] Mix the alginate material with clean water and place it on a separate tray. After anesthetizing the rat, place the rat in a prone position, place the separate tray containing the mixed alginate material in the rat's mouth, and make an impression so that the impression of the teeth and oral tissue is reflected on the impression material. After the impression material is dry, take it out of the rat's mouth to obtain an alginate impression with the shape of the teeth in the rat's mouth.

[0030] S3.Mold filling

[0031] After taking the mold, add clean water to the gypsum powder and mix it, put it into the alginate impression, and vibrate to remove bubbles. After air drying for forty minutes, separate the alginate material from the gypsum model to obtain a gypsum model consistent with the tooth morphology in the rat's mouth.

[0032] S4. Making invisible braces

[0033] The retainer laminating machine, invisible appliance film and rat intraoral plaster model were used to laminator to make invisible appliances. The buccal and lingual sides of the invisible appliances opposite to the interproximal spaces between the first and second molars were perforated with a high-speed mobile phone yellow-label bur, and the incisor part of the invisible appliances was ground off, leaving only the root (such as Figure 1 shown).

[0034] S5. Fitting rats with invisible braces

[0035] After the rats were anesthetized, they were fixed on a rat board. First, an orthodontic ligature with a diameter of 0.030 mm was passed through the interproximal space between the first molar and the second molar of the rat to create a passage. Then, a ligature with a diameter of 0.025 mm was passed through the interproximal space between the first molar and the second molar of the rat, and passed through the pre-holes on the buccal and lingual sides of the invisible braces for ligation. Finally, a fluid resin was applied to the tail of the ligature and solidified to prevent the ligature from piercing the rat's mouth.

[0036] In summary, the experimental model of invisible braces worn in the mouth designed for rats provided by the present invention not only overcomes the problem in traditional experimental methods that invisible braces cannot be worn for a long time after the rat incisors have partially grown, and the physical damage to the rat teeth caused by the wearing process, but also successfully maintains the original natural structure and color of the teeth, providing researchers with a new, non-invasive research method. The animal model constructed by the method of the present invention is conducive to obtaining more accurate data with clinical transformation potential, which greatly promotes the progress of animal experimental research in the field of oral orthodontics.

[0037] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for constructing an animal model of wearing an invisible brace in the mouth of a mouse, characterized in that: The method comprises the steps of drilling holes on the buccal and lingual sides of the invisible appliance opposite to the interproximal spaces between the first molar and the second molar after manufacturing the invisible appliance, grinding off the incisor part of the invisible appliance and retaining only the root part; and passing a ligature wire through the pre-made holes on the buccal and lingual sides of the invisible appliance and ligating the appliance when wearing the appliance.

2. The construction method according to claim 1, characterized in that: Before passing the ligature wire through the pre-hole site on the buccal and lingual sides of the invisible braces, the method also includes passing an orthodontic ligature wire with a larger diameter through the interproximal space between the first molar and the second molar of the rat.

3. The construction method according to claim 2, characterized in that: The ligature wire passing through the pre-holes on the buccal and lingual sides of the invisible orthodontic appliance is an orthodontic ligature wire with a diameter of 0.025 mm.

4. The construction method according to claim 2, characterized in that: The orthodontic ligature wire with a larger diameter is an orthodontic ligature wire with a diameter of 0.030 mm.

5. The construction method according to claim 1, characterized in that: The production of invisible braces also includes the steps of making individual trays, taking impressions and pouring molds.

6. The construction method according to claim 5, characterized in that: Compared with the mouse skull, the size of the individual tray is 3 mm larger than the front end of the incisor, 3 mm larger than the rear end of the molar, and 5 mm larger than the range of the impression on both sides.

7. The construction method according to claim 1, characterized in that: After ligation, the method further comprises the steps of coating the ligation wire tail with a fluid resin and solidifying the resin.

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