Whitening effect and application of artemisia apiacea volatile oil

Abstract

By detecting the inhibition effect of the artemisia apiacea volatile oil on tyrosinase activity, the whitening effect of the artemisia apiacea volatile oil is proved, and the invention belongs to the fields of medicines and cosmetics. Meanwhile, the invention provides a formula and a preparation process of various cosmetics containing the artemisia apiacea volatile oil. The invention not only provides theoretical support for the application of the artemisia apiacea volatile oil, but also provides an example of practical application.

Description

Technical Field

[0001] The invention relates to the fields of medicine and cosmetics, and in particular to the whitening effect of Artemisia annua volatile oil and its application. Technical Background

[0002] Artemisia annua is a traditional Chinese herbal medicine, among which artemisinin is an effective ingredient for antimalarial. Chinese scientist Tu Youyou won the 2015 Nobel Prize in Physiology or Medicine for her research results on artemisinin, becoming the first Chinese to win the Nobel Prize in Natural Science. In the process of extracting artemisinin from Artemisia annua for antimalarial purposes, a large amount of Artemisia annua volatile oil (AN+) is not utilized and is wasted. The Artemisia annua volatile oil is an active ingredient obtained by further purifying the residual substances in the artemisinin extraction process. In recent years, research on Artemisia annua has mainly focused on artemisinin and its derivatives, but research on Artemisia annua volatile oil is less.

[0003] In 2019, Shanghai Kunming Pharmaceutical Group conducted research on the application of Artemisia annua volatile oil in cosmetics, exploring the quality control, efficacy research, formula application and other properties of Artemisia annua volatile oil, and opened up a new direction for Artemisia annua volatile oil in the field of cosmetics. In vitro studies have shown that Artemisia annua volatile oil can upregulate the expression of skin barrier-related genes (filament aggregate protein gene FLG) to enhance the skin barrier and repair skin sensitivity; at the same time, it can inhibit the release of multiple inflammatory mediators (IL-6, IL-8 and TSLP) induced by lipopolysaccharide (LPS) and Staphylococcus aureus, and has anti-inflammatory and soothing effects [1]. Artemisia annua volatile oil can also alleviate the degree of skin lesions in mice (skin disease model) and reduce the inflammatory factors in mouse serum. It has certain therapeutic significance for skin diseases such as atopic dermatitis, skin barrier damage, and psoriasis [2-8]. In addition, human clinical trials have found that after Artemisia annua volatile oil is incorporated into skin care products, products such as lotions, essences, emulsions, and creams containing Artemisia annua volatile oil can relieve skin problems such as sensitivity and redness [9,10].

[0004] With the development of society and the change of aesthetic concepts, people's pursuit of white skin has become stronger. Melanin is the main factor affecting skin color, and its distribution and content in the skin are directly related to the depth of skin color. Melanin is a natural pigment synthesized by melanocytes in the skin, which plays an important role in protecting the skin from ultraviolet damage; however, excessive production or uneven distribution of melanin can cause problems such as spots and freckles, affecting the beauty of the skin.

[0005] Therefore, whitening cosmetics came into being. Their main mechanism of action is to improve skin color by inhibiting the production of melanin or accelerating its metabolism. Currently, most whitening products on the market contain ingredients such as vitamin C, arbutin, and kojic acid. These ingredients reduce the synthesis of melanin by inhibiting the activity of tyrosinase. Tyrosinase is a key enzyme in the process of melanin production. When its activity is inhibited, it can effectively slow down the production of melanin.

[0006] Although existing whitening products can improve skin color to a certain extent, consumers' demand for safer, more effective and more natural whitening ingredients has never stopped. Therefore, developing new whitening ingredients, exploring their mechanisms of action, and improving their application effects in whitening products have become an important direction in the field of cosmetics research and development. In this process, in-depth research on the mechanism of melanin production and continuous screening and optimization of whitening ingredients will bring people a brighter and more even skin color, satisfying the pursuit of beauty in modern society.

[0007] At present, there are few studies on the skin whitening effect of Artemisia annua volatile oil. Artemisia annua volatile oil has a good effect on relieving skin inflammation and may have a certain inhibitory effect on inflammatory pigmentation of the skin. Therefore, exploring whether Artemisia annua volatile oil has a whitening effect has great application prospects. Summary of the invention

[0008] The invention confirms the whitening effect of Artemisia annua volatile oil by detecting the inhibitory effect of Artemisia annua volatile oil on tyrosinase activity, belongs to the field of medicine and cosmetics, and provides a variety of formulas and preparation processes of cosmetics containing Artemisia annua volatile oil. The invention not only provides theoretical support for the application of Artemisia annua volatile oil, but also provides examples of practical application.

[0009] In one aspect, the present invention provides a use of Artemisia annua volatile oil for preparing an agent for improving the whitening effect of a product.

[0010] Artemisia annua volatile oil is further processed from the "waste" generated during the production of artemisinin. Because of its superior anti-inflammatory function, it is widely used in the fields of skin care and antibacterial, but it is still unknown whether Artemisia annua volatile oil has other functions. The exploration of the functions of Artemisia annua volatile oil can not only provide a certain theoretical basis for its application, but also increase the economic benefits of the Artemisia annua processing industry. The present invention confirms that Artemisia annua volatile oil can significantly inhibit tyrosinase activity through in vitro enzyme activity assay experiments, thereby giving the product superior whitening effect.

[0011] Furthermore, the use concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

[0012] Furthermore, the whitening effect is achieved by inhibiting the activity of tyrosinase.

[0013] Furthermore, the whitening effect is achieved by inhibiting melanin production.

[0014] Furthermore, the whitening effect is achieved by inhibiting the generation of pigmentation spots.

[0015] Furthermore, the products include but are not limited to skin topicals, medicines and health foods.

[0016] Furthermore, the skin topical preparation includes but is not limited to eye cream, lotion, essence, toner, gel, facial mask, facial cleanser, facial honey, facial foam, sunscreen, shower gel, spray, etc.

[0017] On the other hand, the present invention provides a use of Artemisia annua volatile oil to improve the whitening effect of a product by inhibiting the activity of tyrosinase.

[0018] Furthermore, the use concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

[0019] Furthermore, the products include but are not limited to skin topicals, medicines and health foods.

[0020] On the other hand, the present invention provides a use of a composition for preparing an agent for improving the whitening effect of a product, wherein the composition comprises any one or more of Artemisia annua volatile oil, octadecenedioic acid, and tranexamic acid.

[0021] Furthermore, the composition is a composition of Artemisia annua volatile oil and octadecenedioic acid, a composition of Artemisia annua volatile oil and tranexamic acid, a composition of octadecenedioic acid and tranexamic acid, and a composition of Artemisia annua volatile oil, octadecenedioic acid and tranexamic acid.

[0022] The present invention confirms that except for the combination of octadecenedioic acid and tranexamic acid, the other three combinations mentioned above have a synergistic effect, and the degree of synergy of the combination of Artemisia annua volatile oil and octadecenedioic acid is greater; the combination of Artemisia annua volatile oil, octadecenedioic acid and tranexamic acid has the best inhibitory effect on tyrosinase activity.

[0023] The beneficial effects of the present invention include:

[0024] 1. The present invention confirms that Artemisia annua volatile oil can significantly inhibit tyrosinase activity, and as the concentration increases, the inhibitory effect becomes more significant. The inhibition rate of 3% Artemisia annua volatile oil has reached 63.69%, indicating that Artemisia annua volatile oil can reduce the production of melanin and spots by inhibiting tyrosinase activity, and thus has a whitening effect;

[0025] 2. The present invention proves that the combination of Artemisia annua volatile oil and octadecenedioic acid and / or tranexamic acid has a synergistic effect on whitening effect, and the composition containing the above three ingredients has the best effect; when the composition contains only two chemical components, the synergistic effect of the combination of Artemisia annua volatile oil and octadecenedioic acid is better than that of the combination of Artemisia annua volatile oil and tranexamic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 : The inhibitory effect of kojic acid (positive control) on tyrosinase activity;

[0028] Figure 2 : Inhibitory effect of Artemisia annua volatile oil on tyrosinase activity. DETAILED DESCRIPTION

[0029] The present invention is further elaborated in detail below in conjunction with the accompanying drawings and specific embodiments of the specification. The embodiments are only used to explain the present invention and are not used to limit the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention.

[0030] The Artemisia annua volatile oil described in the present invention is prepared according to the Artemisia annua volatile oil preparation method described in Chinese patents CN116725905A, CN116725906A, CN116725907A, and CN116731785A; the test methods used in the following examples are conventional methods unless otherwise specified;

[0031] The reagents used are as follows: L-DOPA (Sigma), tyrosinase (Sigma, T3824), disodium hydrogen phosphate dodecahydrate (Sinopharm), citric acid monohydrate (Sinopharm), kojic acid (Yuanye Biotechnology), octadecenedioic acid (Heda Chemicals (Shanghai) Co., Ltd.), and tranexamic acid (Aminsdi (China) Co., Ltd.); the equipment used are as follows: digital constant temperature water bath (Changzhou Guohua, HH-4A), microplate reader (BioTek, Epoch); the other materials and reagents used, unless otherwise specified, are reagents and materials that can be obtained from commercial channels.

[0032] Example 1: Artemisia annua volatile oil can significantly inhibit tyrosinase activity

[0033] Tyrosinase is a key enzyme in the biosynthetic pathway of melanin, which participates in two reactions of melanin synthesis: the first step is to hydroxylate monophenol to diphenol; the second step is to oxidize o-diphenol to o-diquinone, which then becomes melanin after several steps of reaction. Under the condition of pH = 6.8, tyrosinase can directly catalyze tyrosine to produce melanin, and the absorbance value is measured at a wavelength of 475nm to evaluate the activity of tyrosinase. In summary, this embodiment uses a biochemical method to detect the inhibition rate of the sample on tyrosinase activity to evaluate the whitening effect of the sample. The specific steps of tyrosinase enzyme activity determination are as follows:

[0034] (1) Preparation of positive control kojic acid solution

[0035] Kojic acid was originally extracted from fungi such as Aspergillus oryzae. Its main use is for skin whitening and lightening spots. Its mechanism of action is to reduce the synthesis of melanin by inhibiting the activity of tyrosinase. A certain volume of kojic acid powder was dissolved in sodium hydrogen phosphate-citric acid buffer (pH=6.8) to prepare kojic acid working solutions with concentrations of 1.00 mg / mL, 0.20 mg / mL, 0.040 mg / mL, 0.008 mg / mL, and 0.0016 mg / mL.

[0036] (2) Tyrosinase activity assay

[0037] The sample was prepared into a test solution of corresponding concentration, and the reaction system was prepared according to the components in Table 1 (without adding tyrosinase solution) and the addition amount thereof, and mixed; the reaction system was placed in a 37°C water bath and reacted at a constant temperature for 5 minutes; the tyrosinase solution was added to the C and T tubes respectively, and the reaction was continued in a 37°C water bath for 10 minutes; after the reaction, the absorbance (OD) value was read at 475nm, and 3 mechanical replicates were set for each sample tube to calculate the activity inhibition rate of the sample on tyrosinase, the inhibition rate (%) = 1-(T-T0) / (C-C0), wherein T is the average absorbance value of the sample tube (3 mechanical replicates), that is, the absorbance value of the solution after the sample reacts with tyrosinase; T0 is the average absorbance value of the sample background (3 mechanical replicates); C is the average absorbance value of the enzyme reaction tube (3 mechanical replicates), that is, the absorbance value of the reaction of tyrosinase and DOPA when no sample is added; C0 is the average absorbance value of the solvent background (3 mechanical replicates).

[0038] Table 1 Enzyme activity assay system

[0039] T-sample tube T0-Sample Tube C-Tyrosinase Tube C0-Solvent Background Sample solution (μL) 200 200 / / Sodium hydrogen phosphate-citrate buffer (μL) / 100 200 300 Tyrosinase solution (μL) 100 / 100 / Levodopa solution (μL) 400 400 400 400

[0040] (3) Statistical analysis of results

[0041] GraphPad Prism was used for drawing, and the results were expressed as Mean±SD. The t-test was used for comparison among the groups; statistical analysis was two-tailed, P<0.05 represented a significant difference, and P<0.01 represented an extremely significant difference.

[0042] The specific enzyme activity determination results are shown in Tables 2-3 and Figures 1-2 .

[0043] Table 2 Effect of positive control kojic acid on tyrosinase activity

[0044]

[0045] Table 3 Effect of Artemisia annua volatile oil on tyrosinase activity

[0046]

[0047] At concentrations of 0.10%, 0.16%, 0.33%, 0.55%, 1.00% and 3.00%, the tyrosinase activity inhibition rates of Artemisia annua volatile oil were 6.04%, 9.10%, 22.13%, 27.56%, 48.91% and 63.69%, respectively, and the IC50 value was 1.15%.

[0048] Application Example 1: Preparation method of facial cream containing Artemisia annua volatile oil

[0049] This application example provides a method for preparing a facial cream containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial cream is shown in Table 4.

[0050] Table 4 Formula of cream containing Artemisia annua volatile oil

[0051]

[0052]

[0053] The preparation process of the facial cream is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize for 3-10 minutes with appropriate homogenization strength; stir and cool to 50°C, add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, and cool to 37°C before discharging.

[0054] Application Example 2: Preparation method of eye cream containing Artemisia annua volatile oil

[0055] This application example provides a method for preparing an eye cream containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the eye cream is shown in Table 5.

[0056] Table 5: Formula of eye cream containing Artemisia annua volatile oil

[0057]

[0058]

[0059] The preparation process of the eye cream is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize for 3-10 minutes with appropriate homogenization strength; add phase C, homogenize for 3 minutes until uniform, stir and cool to 50°C; add phase D to adjust the pH to 6-6.5, stir and cool to 40°C; add phase E and stir thoroughly until the material is uniform, and cool to 37°C.

[0060] Application Example 3: Preparation method of emulsion containing Artemisia annua volatile oil

[0061] This application example provides a method for preparing an emulsion containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the emulsion is shown in Table 6.

[0062] Table 6 Formula of Artemisia annua volatile oil emulsion

[0063]

[0064] The preparation process of the emulsion is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize with appropriate homogenization force for 3-10 minutes; stir and cool to 50°C, add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, and cool to 37°C before discharging.

[0065] Application Example 4: Preparation method of essence containing Artemisia annua volatile oil

[0066] This application example provides a method for preparing an essence containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the essence is shown in Table 7.

[0067] Table 7 Formula containing Artemisia annua volatile oil essence

[0068]

[0069]

[0070] The preparation process of the essence is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization; add phase C, homogenize with appropriate homogenization force for 3-10 minutes, stir and cool to 50°C; add phase D to adjust the pH to 6-6.5, stir and cool to 40°C; add phase E and stir thoroughly until the material is uniform, cool to 37°C and then discharge.

[0071] Application Example 5: Preparation method of toner containing Artemisia annua volatile oil

[0072] This application example provides a method for preparing a toner containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the toner is shown in Table 8.

[0073] Table 8 Formula of toner containing Artemisia annua volatile oil

[0074]

[0075] The preparation process of toner water is as follows: mix phase A, heat, stir until dissolved and transparent, add phase B after cooling to adjust the pH to 6-6.5; add phase C, stir and mix until the material is uniform, and filter out the material.

[0076] Application Example 6: Preparation method of gel containing Artemisia annua volatile oil

[0077] This application example provides a method for preparing a jelly containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the jelly is shown in Table 9.

[0078] Table 9 Formula containing Artemisia annua volatile oil gel

[0079]

[0080] The preparation process of the gel is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B into phase A under homogenization; add phase C, homogenize with appropriate homogenization force for 3-10 minutes, stir and cool to 50°C; add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, cool to 37°C and then discharge.

[0081] Application Example 7: Preparation method of facial mask containing Artemisia annua volatile oil

[0082] This application example provides a method for preparing a facial mask containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial mask is shown in Table 10.

[0083] Table 10 Formula of facial mask containing Artemisia annua volatile oil

[0084]

[0085]

[0086] The preparation process of the facial mask is as follows: mix phase A, heat, stir until dissolved and transparent, add phase B after cooling to adjust the pH to 6-6.5; add phase C, stir and mix until the material is uniform and then discharge.

[0087] Application Example 8: Preparation method of facial cleanser containing Artemisia annua volatile oil

[0088] This application example provides a method for preparing a facial cleanser containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial cleanser is shown in Table 11.

[0089] Table 11 contains the formula of the Artemisia annua volatile oil facial cleanser

[0090]

[0091] The preparation process of the facial cleanser is as follows: mix phase A, heat to 75°C, and stir until dissolved and transparent; add phase B to adjust the pH to 6.5-7, stir and cool to 50°C; add phase C, start controlled cooling and crystallization, and stop stirring and discharge after the temperature drops to 37°C.

[0092] Application Example 9: Preparation method of facial cleansing foam containing Artemisia annua volatile oil

[0093] This application example provides a method for preparing a cleansing foam containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the cleansing foam is shown in Table 12.

[0094] Table 12 Formula of cleansing foam containing Artemisia annua volatile oil

[0095]

[0096]

[0097] The preparation process of the cleansing foam is as follows: heat and mix phase A until it becomes transparent, and after cooling, add phase B to adjust the pH to 6.5-7; add phase C, stir evenly, and then discharge.

[0098] Application Example 10: Preparation method of spray containing Artemisia annua volatile oil

[0099] This application example provides a method for preparing a spray containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the spray is shown in Table 13.

[0100] Table 13 contains the formula of Artemisia annua volatile oil spray

[0101]

[0102] The preparation process of the spray is as follows: Phase A is heated, mixed and stirred until transparent, after cooling, Phase B is added, stirred and mixed until the material body is uniform, and the material is filtered out.

[0103] Example 2: Inhibitory effect of Artemisia annua volatile oil composition on tyrosinase activity

[0104] In this example, the whitening effect of the sample to be tested is evaluated by detecting the activity of tyrosinase. The specific operation is the same as that described in Example 1. The specific results are shown in Table 14.

[0105] Table 14 Effects of different chemicals on tyrosinase activity

[0106] Group Average inhibition rate SD 1mg / mL kojic acid 91.78％ 0.55％ 0.10% Artemisia annua essential oil 6.04％ 0.26％ 1% octadecenedioic acid 15.33％ 0.23％ 2% Tranexamic Acid 12.45％ 0.50％ 0.10% Artemisia annua essential oil + 1% octadecenedioic acid 34.44％ 0.34％ 0.10% Artemisia annua essential oil + 2% Tranexamic acid 26.22％ 0.46％ 1% octadecenedioic acid + 2% tranexamic acid 25.62％ 0.67％ 0.10% Artemisia annua essential oil + 1% octadecenedioic acid + 2% tranexamic acid 65.17％ 0.38％

[0107] As shown in Table 14, the combination of Artemisia annua volatile oil and octadecenedioic acid or tranexamic acid can further improve the inhibition rate of tyrosinase activity, indicating that the composition of Artemisia annua volatile oil and octadecenedioic acid or tranexamic acid has a synergistic effect, and the synergistic effect of the composition of Artemisia annua volatile oil and octadecenedioic acid is better than that of the combination of Artemisia annua volatile oil and tranexamic acid; and when the composition contains Artemisia annua volatile oil, octadecenedioic acid and tranexamic acid at the same time, the inhibitory effect is the best, indicating that the combination of these three chemical substances has a synergistic effect. In summary, it is preferred that Artemisia annua volatile oil, octadecenedioic acid and tranexamic acid are added to whitening products; when the composition contains only two chemical components, Artemisia annua volatile oil and octadecenedioic acid are preferably combined.

[0108] The present invention also evaluates the whitening effect of Artemisia annua volatile oil at a concentration of 0.00001-20% (v / v), and the effect is consistent with that described in Examples 1-2.

[0109] It is understandable that other positive controls can be used when detecting tyrosinase activity, not limited to kojic acid; other experimental models can also be used to determine the whitening effect, such as detecting the content of melanin in the melanocyte model; Artemisia annua volatile oil can be combined with different formula systems and then used in facial creams, eye creams, lotions, essences, toners, gels, facial masks, facial cleansers, facial honey, facial cleansing foam, sunscreen, shower gel, spray and other products.

[0110] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention; therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is limited by the attached claims rather than the above description. Therefore, it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the present invention, and any figure marks in the claims should not be regarded as limiting the claims involved.

[0111] 1.Kan, T., et al., Artemisia Naphta: A novel oil extract for sensitive and acne prone skin. Annals of Dermatological Research, 2021.5(1):p.022-029.

[0113] 2.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on atopic dermatitis mouse model based on Th1 / Th2 balance. Medical Aesthetics, 2022(10): p.1-5.

[0115] 3.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on skin barrier damage model mice and skin microecology. Medical Aesthetics, 2022.31(16):p.1-7.

[0117] 4.Huang Hu, et al., Intervention effect of artemisia annua oil on psoriasis mouse model. Chinese Journal of Integrated Traditional and Western Medicine Dermatology and Venereology, 2024.23(01):p.1-5.

[0119] 5.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on mouse acne model and skin microecology. Chinese Journal of Leprosy and Skin Diseases, 2024.40(09):p.624-629.

[0121] 6.Huang Hu, et al., Study on the efficacy and mechanism of artemisia annua oil on acne in mice. Journal of Dermatology and Venereology, 2024.31(06):p.374-380. 7.Huang Hu, et al., Effects of artemisia annua oil on guinea pig seborrheic dermatitis model. Chinese Journal of Leprosy and Skin Diseases, 2024.40(08):p.555-559. 8. Huang, H., et al., Novel anti-inflammatory Artemisia Naphta oilextract efficacious in in vivo mouse models of atopic dermatitis and psoriasis. Research Square, 2023.

[0122] 9. Qin Ziyan, et al., The development history of Kunming Artemisia annua raw materials and new applications in sensitive skin care. Chinese Cosmetics, 2022(10): p.122-126.10. Wang Sha, et al., The effect and safety of skin care products containing Artemisia annua volatile oil in people with sensitive skin. Journal of Dermatology and Venereology, 2024.31(02): p.67-76.

Claims

1. Use of Artemisia annua volatile oil in preparing a reagent for improving the whitening effect of a product.

2. The use according to claim 1, characterized in that The usage concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

3. The use according to claim 1, characterized in that The whitening effect is to inhibit the activity of tyrosinase.

4. The use according to claim 1, characterized in that The whitening effect is to inhibit melanin production.

5. The use according to claim 1, characterized in that The whitening effect is to inhibit the generation of spots.

6. The use according to claim 5, characterized in that The products include but are not limited to skin topicals, medicines and health foods.

7. The use according to claim 6, characterized in that The skin topical preparations include but are not limited to eye cream, lotion, essence, toner, gel, facial mask, facial cleanser, facial honey, facial foam, sunscreen, shower gel, spray, etc.

8. A use of Artemisia annua volatile oil to improve the whitening effect of a product by inhibiting the activity of tyrosinase.

9. The use according to claim 8, characterized in that The usage concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

10. Use of a composition for preparing an agent for improving the whitening effect of a product, characterized in that: The composition comprises any one or more of Artemisia annua volatile oil, octadecenedioic acid and tranexamic acid.

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