Traditional Chinese medicine composition, traditional Chinese medicine preparation and feed for preventing and treating gout of goslings as well as preparation method and application of traditional Chinese medicine composition and traditional Chinese medicine preparation

Abstract

The invention provides a traditional Chinese medicine composition for preventing and treating gout of goslings, a traditional Chinese medicine preparation, feed, a preparation method and application, and belongs to the field of livestock breeding. The traditional Chinese medicine composition for preventing and treating gout of goslings can effectively prevent and / or treat gout of goslings caused by GAstV, and can be used for preventing and treating gout of goslings caused by goose astroviruses (the protection rate of a prevention group reaches 80%, and the protection rate of a treatment group reaches 93.33%); the growth performance of the goslings can be remarkably promoted, and the immunity of the goslings can be improved. Moreover, the traditional Chinese medicine composition for preventing and treating gout of goslings is precise and appropriate in formula, simple to prepare and remarkable in effect, and has a great popularization prospect for replacing or reducing use of antibiotics.

Description

Technical Field

[0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a feed for preventing and treating gout in goslings, and a preparation method and application thereof. Background Art

[0002] Goose gout is a disease caused by a large amount of uric acid accumulated in the blood due to various reasons, resulting in urate deposition in the internal organs, subcutaneous connective tissue and joints. The disease is common in goslings within 3 weeks of age, and rarely occurs after 20 days of age. It can occur in different breeds of geese and in all seasons, but it is more common in winter and spring when green feed is scarce. It is more common under low temperature and high humidity conditions. Urate deposition is common in the pericardium, joints and limbs, kidney swelling and whitening, and white lime-like loose feces.

[0003] There are many causes of gout in geese. Among them, goose gout caused by the new goose astrovirus (GAstV) is not only an acute infectious disease characterized by the presence of large amounts of uric acid deposits in the pericardium, kidneys, liver and other internal organs and joints of goslings, but can also spread widely in a short period of time. It is mainly caused by young geese, with rapid onset and severe illness. The mortality rate is as high as 80%. However, there is currently no effective drug to effectively prevent and treat gout caused by GAstV. Summary of the invention

[0004] The object of the present invention is to provide a Chinese medicine composition, Chinese medicine preparation, feed, preparation method and application for preventing and treating gout in goslings. The Chinese medicine composition for preventing and treating gout in goslings of the present invention can effectively prevent and / or treat gout in goslings caused by GAstV.

[0005] The invention provides a traditional Chinese medicine composition for preventing and treating gout in goslings. The traditional Chinese medicine composition comprises the following raw materials for preparation: plantago seed, isatis root, astragalus and siler.

[0006] Preferably, the Chinese medicine composition comprises the following raw materials for preparation in parts by weight:

[0007] 1-10 parts of Plantago seeds, 1-10 parts of Isatis root, 1-10 parts of Astragalus and 1-10 parts of Saposhnikovia divaricata.

[0008] The present invention also provides a traditional Chinese medicine preparation for preventing and treating gout in goslings. The raw materials for preparing the traditional Chinese medicine preparation include the traditional Chinese medicine composition described in the above technical solution and auxiliary materials acceptable in traditional Chinese medicine.

[0009] Preferably, when the dosage form of the traditional Chinese medicine preparation is a decoction, each milliliter of the traditional Chinese medicine preparation contains 1 g of the crude drug.

[0010] The present invention also provides a method for preparing the Chinese medicine preparation described in the above technical solution, comprising the following steps:

[0011] Mixing the raw materials and water, boiling and filtering to obtain a filter residue and a first filtrate;

[0012] Mix the filter residue with water, boil and filter to obtain a second filtrate;

[0013] The first filtrate and the second filtrate are combined and concentrated to obtain the traditional Chinese medicine preparation.

[0014] Preferably, the concentration comprises concentration under reduced pressure.

[0015] Preferably, the mass ratio of the raw material to water is 1:(5-10); the mass ratio of the filter residue to water is 1:(5-10);

[0016] The boiling time ranges from 0.5 to 2 hours.

[0017] Preferably, the raw materials for preparation further include soaking before boiling;

[0018] The soaking time ranges from 0.5 to 1 hour.

[0019] The present invention also provides a feed having the function of preventing and treating gout in goslings, wherein the ingredients of the feed include the Chinese medicine composition described in the above technical solution or the Chinese medicine preparation described in the above technical solution or the Chinese medicine preparation prepared by the preparation method described in the above technical solution and a basic diet.

[0020] The present invention also provides the use of the Chinese medicine composition described in the above technical solution, the Chinese medicine preparation described in the above technical solution, the Chinese medicine preparation prepared by the preparation method described in the above technical solution, or the feed described in the above technical solution in any one of the effects ① to ③:

[0021] ① Reduce the mortality of goslings caused by goose astrovirus;

[0022] ② Improve the growth performance of goslings;

[0023] ③ Enhance the immunity of goslings.

[0024] The invention provides a Chinese medicine composition for preventing and treating gout in goslings. The Chinese medicine composition comprises plantago, isatis root, astragalus and siler. Among them, plantago: sweet, slightly cold, enter the liver, kidney, lung, small intestine meridian, diuretic and stranguria, has the effects of penetrating dampness and stranguria, clearing heat and diuresis, removing phlegm, and improving eyesight; isatis root: bitter, cold, enter the heart and stomach meridians, has the effects of cooling blood and relieving sore throat, clearing heat and detoxifying; astragalus: sweet, warm, enter the lung and spleen meridians, has diuretic and swelling, replenishing qi and strengthening the exterior, removing toxins and draining pus, astringing sores and promoting tissue regeneration, and has the effects of replenishing qi and promoting body fluid; siler: sweet, warm, enter the bladder, liver, spleen meridians, has the effects of removing dampness, stopping spasm, relieving exterior wind, and has the effects of pungent and warm exterior relieving. The Chinese medicine composition of the present invention is used in the breeding process of goslings, which can not only be used to prevent and treat gout caused by goose astrovirus (the protection rate of the prevention group is 80%, and the protection rate of the treatment group is 93.33%); it can also have a significant effect on the growth performance of goslings and improve the immunity of goslings. The present invention has a precise formula, simple preparation, and significant effect, and has great promotion prospects for replacing or reducing the use of antibiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 A comparison chart of the effects of different treatment groups on the heart and liver of goslings in the initial screening test provided by the present invention;

[0027] Figure 2 A comparison chart of the effects of different treatment groups on the kidneys of goslings in the initial screening test provided by the present invention;

[0028] Figure 3 The autopsy diagram of the dead goslings in the blank control group of the initial screening test provided by the present invention; wherein A is the autopsy diagram of the heart and liver of the dissected goslings; B is the autopsy diagram of the kidneys of the dissected goslings;

[0029] Figure 4 A comparison chart of the effects of different treatment groups on the heart and liver of goslings in the rescreening test provided by the present invention;

[0030] Figure 5 A comparison chart of the effects of different treatment groups on the kidneys of goslings in the rescreening test provided by the present invention;

[0031] Figure 6 The autopsy diagram of the dead goslings in the blank control group of the rescreening test provided by the present invention; wherein A is the autopsy diagram of the heart and liver of the dissected goslings; B is the autopsy diagram of the kidneys of the dissected goslings;

[0032] Figure 7 This is a comparison chart of the effects of different treatment groups on the heart and liver of goslings in the dosage screening test provided by the present invention;

[0033] Figure 8 A comparison chart of the effects of different treatment groups on the kidneys of goslings in the dosage screening test provided by the present invention;

[0034] Fig. 9 This is a comparison chart of the viral loads in the kidneys, livers, and feces of goslings in different treatment groups 14 days after infection provided by the present invention. DETAILED DESCRIPTION

[0035] The present invention provides a Chinese medicine composition for preventing and treating gout in goslings, the Chinese medicine composition comprising the following raw materials for preparation: Plantago, Radix Isatidis, Radix Astragali and Radix Saposhnikoviae. In a specific embodiment, the Chinese medicine composition may comprise the following raw materials for preparation in parts by weight: 1-10 parts of Plantago, 1-10 parts of Radix Isatidis, 1-10 parts of Radix Astragali and 1-10 parts of Radix Saposhnikoviae.

[0036] In a specific embodiment, the use ratio of the plantago seed, isatis root, astragalus and saposhnikovia can be 1:1:1:1. In a specific embodiment, the plantago seed, isatis root, astragalus and saposhnikovia can be conventional commercial products. In a specific embodiment, the plantago seed is sweet, slightly cold in nature, and enters the liver, kidney, lung, and small intestine meridians, has diuresis and stranguria, and has the effects of penetrating dampness and stranguria, clearing heat and diuresis, removing phlegm, and improving eyesight. In a specific embodiment, the isatis root is bitter, cold in nature, enters the heart and stomach meridians, and has the effects of cooling blood and relieving sore throat, clearing heat and detoxifying. In a specific embodiment, the astragalus is sweet, warm in nature, enters the lung and spleen meridians, has diuresis and swelling, replenishes qi and consolidates the exterior, removes toxins and discharges pus, astringes sores and promotes muscle growth, and has the effects of invigorating qi and promoting body fluid. In a specific embodiment, the saposhnikovia flavor is sweet, warm in nature, enters the bladder, liver, and spleen meridians, has the effects of winning dampness, stopping spasms, relieving the exterior and removing wind, and has the effects of pungent and warm exterior. The invention combines the above-mentioned drugs in a compatible manner, which can clear away heat, promote diuresis, dispel wind and dampness, relieve numbness and relieve pain, and can also clear away heat and detoxify, replenish qi and strengthen the exterior, and enhance immunity, and is suitable for the prevention and treatment of gout in goslings.

[0037] The present invention also provides a Chinese medicine preparation for preventing and treating gout in goslings, wherein the raw materials for preparing the Chinese medicine preparation include the Chinese medicine composition described in the above technical solution and an auxiliary material acceptable in Chinese medicine. In a specific embodiment, the Chinese medicine composition can be crushed into a powder by crushing the raw medicine and added to the feed.

[0038] In a specific embodiment, when the dosage form of the Chinese medicine preparation is a decoction, each milliliter of the Chinese medicine preparation may contain 1g of the crude drug. In a specific embodiment, the decoction may be mixed into the basic diet of goslings for goslings to take, and the amount of the decoction added may be 1% of the weight of the basic diet. In a specific embodiment, the decoction may also be added to drinking water for goslings to take, and the amount of the decoction added may be 0.5% of the weight of the drinking water for goslings.

[0039] The present invention also provides a method for preparing the Chinese medicine preparation described in the above technical solution, comprising the following steps:

[0040] Mixing the raw materials and water, boiling and filtering to obtain a filter residue and a first filtrate;

[0041] Mix the filter residue with water, boil and filter to obtain a second filtrate;

[0042] The first filtrate and the second filtrate are combined and concentrated to obtain the traditional Chinese medicine preparation.

[0043] The present invention mixes raw materials and water, boils and filters to obtain filter residue and a first filtrate. In a specific embodiment, the raw materials for preparation further include soaking before boiling; the soaking time may include 0.5 to 1 hour. In a specific embodiment, the mass ratio of the raw materials and water can be 1: (5 to 10). In a specific embodiment, the boiling time may include 0.5 to 2 hours.

[0044] After obtaining the filter residue and the first filtrate, the present invention mixes the filter residue with water, boils and filters to obtain a second filtrate. In a specific embodiment, the mass ratio of the filter residue to water can be 1:(5-10). In a specific embodiment, the boiling time can include 0.5-2h.

[0045] After obtaining the second filtrate, the present invention combines the first filtrate and the second filtrate and concentrates them to obtain the Chinese medicine preparation. In a specific embodiment, the concentration includes reduced pressure concentration. In a specific embodiment, the Chinese medicine preparation can be mixed with feed or drinking water after packaging. In a specific embodiment, the Chinese medicine preparation can be mixed with the basic diet of goslings for goslings to take, and the amount of the Chinese medicine preparation added can be 1% of the mass of the basic diet. In a specific embodiment, the Chinese medicine preparation can also be added to drinking water for goslings to take, and the amount of the Chinese medicine preparation added can be 0.5% of the mass of the drinking water for goslings.

[0046] The present invention also provides a feed having the function of preventing and treating gout in goslings, wherein the ingredients of the feed include the Chinese medicine composition described in the above technical solution or the Chinese medicine preparation described in the above technical solution or the Chinese medicine preparation prepared by the preparation method described in the above technical solution and a basic diet.

[0047] The present invention also provides the use of the Chinese medicine composition described in the above technical solution, the Chinese medicine preparation described in the above technical solution, the Chinese medicine preparation prepared by the preparation method described in the above technical solution, or the feed described in the above technical solution in any one of the effects ① to ③:

[0048] ① Reduce the mortality of goslings caused by goose astrovirus;

[0049] ② Improve the growth performance of goslings;

[0050] ③ Enhance the immunity of goslings.

[0051] To further illustrate the present invention, a Chinese medicine composition, Chinese medicine preparation, feed, preparation method and application for preventing and treating gout in goslings provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0052] Example 1

[0053] Preliminary screening of Chinese herbal formula for preventing and treating gout in goslings.

[0054] 1. Materials.

[0055] The following Chinese medicine formula was prepared according to the mass ratio and then subjected to water extraction process to prepare a water extract containing 1g of crude drug per milliliter.

[0056] Formula ①: Plantago, Isatis, Astragalus (1:1:1);

[0057] Formula ②: Rhizoma Cynanchum, Cinnamomum cassia (1:1);

[0058] Formula ③: Plantago seed, Gentiana macrophylla, Ligusticum chuanxiong, Saposhnikovia divaricata (2:1:1:2);

[0059] Positive control ④: Sini Decoction: Zhigancao, Ganjiang, Fuzi (3:2:3);

[0060] Experimental animals: 300 one-day-old Northeastern goslings.

[0061] Virus: Goose astrovirus allantoic fluid GAstV CH-HNXX-2021(10 6.28 copies / μL; EID 50 For 10 0.33 / 0.2mL).

[0062] 2. Test method.

[0063] 2.1 Preparation of experimental drugs.

[0064] According to the selected mass ratio, use an electronic balance to accurately weigh the Chinese medicines of the above Chinese medicine formulas in sequence. Add 5 times the amount of water and soak for 30 minutes. Boil for 60 minutes on low heat, collect the filtrate, add 5 times the amount of water to the residue, boil for 60 minutes on low heat, combine the filtrates twice, and concentrate under reduced pressure. The final concentration is 1g of crude drug per milliliter of liquid medicine, which can be mixed with materials after packaging. (Note: In the above formula ④, since aconite is toxic, it should be decocted alone for 1 hour when decocting, and then the decocted aconite and the decoction should be mixed with other medicines for decocting).

[0065] 2.2 Experimental groups and treatments.

[0066] Three hundred one-day-old goslings were randomly divided into 10 groups, with 3 replicates in each group and 10 goslings in each replicate. The 10 groups of goslings were specifically divided into a blank control group, a model group, 4 Chinese medicine prevention groups corresponding to different Chinese medicines, and 4 Chinese medicine treatment groups corresponding to different Chinese medicines. It was divided into a blank control group, a model group, a Chinese medicine prevention group, and a Chinese medicine treatment group. The specific grouping and administration methods are shown in Table 1, and the drug composition of each group is shown in Table 2. Among them, Group A was the blank control group, fed with basic feed and drank tap water. Groups B to D were orally inoculated with goose astrovirus allantoic fluid (10 6.28 copies / μL; 0.2mL), among which group B was the model group, fed with basic feed; group C (C1, C2, C3, C4) was the Chinese medicine prevention group, and Chinese medicine liquid was mixed into the feed while attacking the virus; group D (D1, D2, D3, D4) was the Chinese medicine treatment group, and after the model was successfully made (after the 3rd day of attacking the virus), 1% of the Chinese medicine liquid of the basic diet was added into the feed.

[0067] Table 1 Grouping and administration method

[0068] Grouping deal with A. Blank control group - Model group B On the first day, the virus was attacked C Chinese medicine prevention group The virus was challenged on the first day, and the drug was continuously administered until the 14th day. D Chinese medicine treatment group The virus was challenged on the first day, and the drug was administered on the fourth day, and the drug was administered continuously until the 14th day.

[0069] Table 2 Drug composition of each group

[0070] Group drug C1 and D1 Plantago, Isatis, Astragalus C2 and D2 Sichuan Achyranthes, Guizhi C3 and D3 Plantago, Gentiana macrophylla, Ligusticum chuanxiong, Saposhnikovia divaricata C4 and D4 Sini Decoction (roasted licorice, dried ginger, aconite)

[0071] 2.3 Feeding and management.

[0072] The experimental period was 14 days, and artificial control methods were used during the experiment to control the temperature and humidity of the feeding. The goslings were fed in cardboard boxes at a temperature of 28±2℃ and a humidity of 80±5%. During the experiment, the goslings were free to eat and drink water, and feed was added and water was changed regularly every day. All experimental procedures were reviewed and approved by the Animal Care and Use Committee of Henan Agricultural University.

[0073] 2.4 Clinical observation

[0074] During the experiment, the weight, mental state, defecation, death, etc. of each group of geese were observed and recorded. The dead goslings were autopsied, and at the end of the experiment, 5 goslings were randomly selected from each group for autopsy.

[0075] 2.5 Mortality and growth performance determination.

[0076] The mortality rate, daily feed intake, and body weight of goslings in each group were recorded, and the average daily feed intake (ADFI), average daily weight gain (ADG), and feed-to-weight ratio (F / G) were calculated. Among them, average daily weight gain = (final weight - initial weight) / number of experimental days; average daily feed intake = total feed intake / (number of goslings in each group × number of experimental days); feed-to-weight ratio = average daily feed intake / average daily weight gain.

[0077] 2.6 Data analysis.

[0078] The experimental results are expressed as mean ± standard deviation (Mean ± SD). Analysis of variance (ANOVA), significance of difference (Duncan multiple comparison method) and graphing were all performed using Prism 8.0.2. P < 0.05 indicated a significant difference, and P < 0.01 indicated a significant difference.

[0079] 3.Test results.

[0080] 3.1 Clinical observation

[0081] Goslings in the challenge group began to show clinical symptoms on the third day and died on the fifth day, mainly with depression and white loose feces. The peak period of death was from the third to the ninth day after the challenge, with a total of 16 deaths and a mortality rate of 53.3%. Figure 1 and Figure 2 As shown, Figure 1 This is a comparison chart of the effects of different treatment groups on the heart and liver of goslings. Specifically, the heart and liver of the blank control group (A) were normal; the heart and liver of the model group (B) had urate deposition in the heart and liver; the hearts and livers of the Chinese medicine prevention group and the Chinese medicine treatment group were all normal. Figure 2 This is a comparative chart of the effects of different treatment groups on the kidneys of goslings. Specifically, the blank control group (A) had normal kidneys; the model group (B) had enlarged and mottled kidneys, with white urate deposits in the ureters on both sides; the Chinese medicine prevention group and the Chinese medicine treatment group (C1 and D1) that were administered according to formula ① had normal kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C2 and D2) that were administered according to formula ② had slight bleeding and swelling in the kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C3 and D3) that were administered according to formula ③ had slight bleeding and swelling in the kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C4 and D4) that were administered according to formula ④ of the positive control had slight bleeding and swelling in the kidneys.

[0082] It can be seen that the model group had obvious urate deposits on the surface of the heart and liver, enlarged kidneys with bleeding spots, and white urate deposits on both sides of the ureter, while the goslings in the control group had no lesions in all organs after autopsy. That is, with the feeding of Chinese medicine, the goslings in the Chinese medicine test group (C1-3 and D1-3) and the positive control group (C4 and D4) were in better spirits than the virus control group, and the degree of kidney damage was lower than that of the virus control group.

[0083] 3.2 Effect of Chinese medicine prescription on the production performance of goslings.

[0084] Table 3 Effects of different treatment groups on the mortality of goslings

[0085] Group Deaths Survival Case fatality rate% A 2 28 6.67 B 16 14 53.33 C1 13 17 43.33 C2 15 15 50 C3 16 14 53.33 C4 9 21 30 D1 8 22 26.67 D2 16 14 53.33 D3 16 14 53.33 D4 8 22 26.67

[0086] The results of the effects of different treatment groups on the mortality of goslings are shown in Table 3. As can be seen from Table 3, a total of 16 goslings died in the model group (B) challenged with poison, with a mortality rate of 53.3%, while group ① (groups C1 and D1) was the best group in terms of both prevention and treatment, and its treatment effect was better than the prevention effect. The blank control group was raised alone without challenge, and only 2 geese died during the whole process. The autopsy results were as follows: Figure 3 As shown, no gout lesions were found in the heart, liver, and kidneys of geese, ruling out gout infection in geese, which may be caused by other factors.

[0087] Table 4 Effects of different treatment groups on the growth performance of goslings

[0088]

[0089] Note: * indicates comparison with group B, P < 0.05.

[0090] The results of the effects of different treatment groups on the growth performance of goslings are shown in Table 4. It can be seen from Table 4 that compared with the model group, the average daily weight gain and final weight of goslings in group ① increased, and the feed-to-weight ratio decreased, which was not much different from the blank control group. And the treatment effect of group ① was the same as that of positive drug group ④, but since these four groups of Chinese medicine prescriptions did not achieve the ideal effect in preventing and treating gout in geese, the next step of the experiment was to improve the best performing group ① and positive drug group ④ among the above Chinese medicine prescriptions and then conduct the experiment.

[0091] Example 2

[0092] Rescreening of traditional Chinese medicine formula for preventing and treating gout in goslings.

[0093] 1. Materials.

[0094] The following Chinese medicine formula was prepared according to the mass ratio and then subjected to water extraction process to prepare a water extract containing 1g of crude drug per milliliter (crude drug amount 1g / mL).

[0095] Formula ①: roasted licorice root, dried ginger, aconite root, astragalus root (3:2:3:2);

[0096] Formula ②: Plantago, Isatis, Astragalus (1:1:1);

[0097] Formula ③: Plantago, Isatis, Astragalus, Saposhnikovia (1:1:1:1);

[0098] Positive control group④: Sini decoction: roasted licorice root, dried ginger, aconite root (3:2:3);

[0099] Experimental animals: 300 one-day-old Northeastern goslings.

[0100] Virus: Goose astrovirus allantoic fluid GAstV CH-HNXX-2021(10 6.28 copies / μL; EID 50 For 10 0.33 / 0.2mL).

[0101] 2. Test method.

[0102] 2.1 Preparation of experimental drugs.

[0103] According to the selected mass ratio, use an electronic balance to accurately weigh the Chinese medicines in the above Chinese medicine formulas in sequence. Add 5 times the amount of water and soak for 30 minutes. Boil for 60 minutes on low heat, collect the filtrate, add 5 times the amount of water to the residue, boil for 60 minutes on low heat, combine the filtrates twice, and concentrate under reduced pressure. The final concentration is 1g of crude drug per milliliter of liquid medicine, which can be mixed with materials after packaging. (Note: In the above formula ①, since aconite is toxic, it should be decocted alone for 1 hour when decocting, and then the decocted aconite and the decoction should be mixed with other medicines for decocting).

[0104] 2.2 Experimental groups and treatments.

[0105] Three hundred one-day-old goslings were randomly divided into 10 groups, with 3 replicates in each group and 10 goslings in each replicate. The 10 groups of goslings were specifically divided into a blank control group, a model group, 4 Chinese medicine prevention groups corresponding to different Chinese medicines, and 4 Chinese medicine treatment groups corresponding to different Chinese medicines. The specific grouping and administration methods are shown in Table 5, and the drug composition of each group is shown in Table 6. Among them, Group A is the healthy control group, fed with basic feed and drinking tap water. Groups B to D were orally inoculated with goose astrovirus allantoic fluid (10 6.28 copies / μL; 0.2mL), among which group B was the model group, fed with basic feed; group C (C1, C2, C3, C4) was the Chinese medicine prevention group, and Chinese medicine liquid was mixed into the feed while attacking the virus; group D (D1, D2, D3, D4) was the Chinese medicine treatment group, and after the model was successfully made (after the 3rd day of attacking the virus), 1% of the Chinese medicine liquid of the basic diet was added into the feed.

[0106] Table 5 Grouping, administration method and drug composition of each group

[0107] Grouping deal with A. Blank control group - Model group B On the first day, the virus was attacked C Chinese medicine prevention group The virus was challenged on the first day, and the drug was continuously administered until the 14th day. D Chinese medicine treatment group The virus was challenged on the first day, and the drug was administered on the fourth day, and the drug was administered continuously until the 14th day.

[0108] Table 6 Drug composition of each group

[0109] Group drug C1 and D1 Roasted Licorice, Dried Ginger, Aconite, Astragalus C2 and D2 Plantago, Isatis, Astragalus C3 and D3 Plantago, Isatis root, Astragalus, Saposhnikovia root C4 and D4 Sini Decoction (roasted licorice, dried ginger, aconite)

[0110] 2.3 Feeding and management.

[0111] The experimental period was 14 days, and artificial control methods were used during the experiment to control the temperature and humidity of the feeding. The goslings were fed in cardboard boxes at a temperature of 28±2℃ and a humidity of 80±5%. During the experiment, the goslings were free to eat and drink water, and feed was added and water was changed regularly every day. All experimental procedures were reviewed and approved by the Animal Care and Use Committee of Henan Agricultural University.

[0112] 2.4 Clinical observation

[0113] During the experiment, the weight, mental state, defecation, death, etc. of each group of geese were observed and recorded. The dead goslings were autopsied, and at the end of the experiment, 5 goslings were randomly selected from each group for autopsy.

[0114] 2.5 Mortality and growth performance determination.

[0115] The mortality rate, daily feed intake, and body weight of goslings in each group were recorded, and the average daily feed intake (ADFI), average daily weight gain (ADG), and feed-to-weight ratio (F / G) were calculated. Average daily weight gain = (final weight - initial weight) / number of experimental days, average daily feed intake = total feed intake / (number of goslings in each group × number of experimental days), feed-to-weight ratio = average daily feed intake / average daily weight gain.

[0116] 2.6 Data analysis.

[0117] The experimental results are expressed as mean ± standard deviation (Mean ± SD). Analysis of variance (ANOVA), significance of difference (Duncan multiple comparison method) and graphing were all performed using Prism 8.0.2. P < 0.05 indicated a significant difference, and P < 0.01 indicated a significant difference.

[0118] 3.Test results.

[0119] 3.1 Clinical observation

[0120] The goslings in the challenge group began to show clinical symptoms and died on the third day, mainly depression and white loose feces. The peak period of death was from the third to the ninth day after the challenge, with a total of 13 deaths and a mortality rate of 43.3%. Figure 4 and Figure 5 As shown, Figure 4 This is a comparison chart of the effects of different treatment groups on the heart and liver of goslings. Specifically, the heart and liver of the blank control group (A) were normal; the heart and liver of the model group (B) had urate deposition in the heart and liver; the hearts and livers of the Chinese medicine prevention group and the Chinese medicine treatment group were all normal. Figure 5 This is a comparative chart of the effects of different treatment groups on the kidneys of goslings. Specifically, the blank control group (A) had normal kidneys; the model group (B) had enlarged and mottled kidneys, with white urate deposits in the ureters on both sides; the Chinese medicine prevention group and the Chinese medicine treatment group (C1 and D1) that were administered according to formula ① had slight kidney bleeding; the Chinese medicine prevention group and the Chinese medicine treatment group (C2 and D2) that were administered according to formula ② had slight kidney bleeding and swelling; the Chinese medicine prevention group and the Chinese medicine treatment group (C3 and D3) that were administered according to formula ③ had normal kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C4 and D4) that were administered according to formula ④ of the positive control had slight kidney bleeding and swelling.

[0121] It can be seen that the model group had obvious urate deposits on the surface of the heart and liver, enlarged kidneys with bleeding spots, and white urate deposits on both ureters, while the control group had no lesions in all organs after autopsy. With the feeding of Chinese medicine, the goslings in the Chinese medicine prevention group and treatment group were in better spirits than the virus control group, and the degree of kidney damage was lower than that of the virus control group.

[0122] 3.2 Effect of Chinese medicine prescription on the production performance of goslings.

[0123] Table 7 Effects of different treatment groups on the mortality of goslings

[0124]

[0125]

[0126] The results of the effects of different treatment groups on the mortality of goslings are shown in Table 7. It can be seen from Table 7 that compared with Group B, Group ③ (Group C3 and Group D3) significantly reduced the mortality rate in both prevention and treatment, and its treatment effect was better than the prevention effect. The blank control group was raised alone without poisoning, and only one goose died during the whole process. The autopsy was as follows Figure 6 As shown, no gout lesions were found in the heart, liver, and kidneys of geese, ruling out gout infection in geese, which may be caused by other factors.

[0127] Table 8 Effects of different treatment groups on the growth performance of goslings

[0128]

[0129] Note: * indicates comparison with group B, P < 0.05.

[0130] The results of the effects of different treatment groups on the growth performance of goslings are shown in Table 8. It can be seen from Table 8 that compared with the model group, the average daily weight gain and final weight of goslings in group ③ increased significantly (P < 0.05), and the feed-to-weight ratio decreased significantly (P < 0.05). Therefore, group ③ is the optimal group for the experiment, and the next step is to screen the dosage of group ③.

[0131] Example 3

[0132] Screening of dosage of traditional Chinese medicine formula for preventing and treating gout in goslings.

[0133] 1. Materials.

[0134] The following Chinese medicine formula was prepared according to the mass ratio and then water-extracted to prepare a 1:1 oral solution (raw drug amount 1 g / mL).

[0135] Positive control: ① Tongfengling (Conoqi Biotechnology); ② Sinitang (Jinrui Biotechnology).

[0136] Chinese medicine formula: Plantago seed, Isatis root, Astragalus, Saposhnikovia divaricata (1:1:1:1).

[0137] Experimental animals: 360 one-day-old Northeastern goslings;

[0138] Virus: Goose astrovirus allantoic fluid GAstV CH-HNXX-2021(10 6.28 copies / μL; EID 50 For 10 0.33 / 0.2mL).

[0139] 2. Test method.

[0140] 2.1 Preparation of experimental drugs.

[0141] According to the selected mass ratio, accurately weigh Radix Isatidis, Radix Astragali, and Radix Saposhnikoviae in turn with an electronic balance. Add 5 times the amount of water and soak for 30 minutes. Boil for 60 minutes on low heat, collect the filtrate, add 5 times the amount of water to the residue, boil for 60 minutes on low heat, combine the filtrates twice, and concentrate under reduced pressure. The final concentration is 1g of crude drug per milliliter of liquid. It can be mixed with materials after packaging.

[0142] 2.2 Experimental groups and treatments.

[0143] 360 one-day-old goslings were randomly divided into 12 groups, with 3 replicates in each group and 10 goslings in each replicate. The 12 groups of goslings were specifically divided into 1 blank control group, 1 model group, 5 Chinese medicine prevention groups and 5 Chinese medicine treatment groups, of which the Chinese medicine prevention group and the Chinese medicine treatment group each contained two positive control groups of commercially available drugs (Tongfengling and Sinitang). The specific administration method is shown in Table 9, among which Group A is the healthy control group, which is fed with basic feed and drinks tap water. Groups B to D were orally inoculated with goose astrovirus allantoic fluid (10 6.28 copies / μL; 0.2mL), among which Group B was the model group, which was fed with basic feed; Group C was the Chinese medicine prevention group, and at the same time as the poison attack, the five Chinese medicine prevention groups were respectively mixed with Chinese medicine liquid (Tongfengling C1, Sinitang C2 or the Chinese medicine formula C3-H, C3-M, C3-L of the present invention) into the gosling feed; Group D was the Chinese medicine treatment group, and after the model was successfully made (3rd day after the poison attack), the five Chinese medicine treatment groups were respectively mixed with Chinese medicine liquid (Tongfengling D1, Sinitang D2 or the Chinese medicine formula D3-H, D3-M, D3-L of the present invention) into the gosling feed. For Group C and Group D, Tongfengling and Sinitang were administered according to the instructions of commercial products, and the Chinese medicine formula of the present invention was mixed into the feed and administered according to three doses of high, medium and low, among which the high dose was 20 mL / kg (C3-H and D3-H), the medium dose was (C3-M and D3-M) 10 mL / kg, and the low dose was 5 mL / kg (C3-L and D3-L).

[0144] Table 9 Grouping and administration method

[0145]

[0146] 2.3 Feeding and management.

[0147] The experimental period was 14 days, and artificial control methods were used during the experiment to control the temperature and humidity of the feeding. The goslings were fed in cardboard boxes at a temperature of 28±2℃ and a humidity of 80±5%. During the experiment, the goslings were free to eat and drink water, and feed was added and water was changed regularly every day. All experimental procedures were reviewed and approved by the Animal Care and Use Committee of Henan Agricultural University.

[0148] 2.4 Clinical observation

[0149] During the experiment, the weight, mental state, defecation, death, etc. of each group of geese were observed and recorded. The dead goslings were autopsied, and at the end of the experiment, 5 goslings were randomly selected from each group for autopsy.

[0150] 2.5 Mortality and growth performance determination.

[0151] The mortality rate, daily feed intake, and body weight of goslings in each group were recorded, and the average daily feed intake (ADFI), average daily weight gain (ADG), and feed-to-weight ratio (F / G) were calculated. Average daily weight gain = (final weight - initial weight) / number of experimental days, average daily feed intake = total feed intake / (number of goslings in each group × number of experimental days), feed-to-weight ratio = average daily feed intake / average daily weight gain.

[0152] 2.6 Determination of immune organ index.

[0153] After the experiment, 5 goslings were selected from each group and slaughtered. The liver, bursa of Fabricius, and spleen were collected, weighed, and the organ index was calculated, and the intestinal length was measured.

[0154] Here, immune organ index = [immune organ mass (mg) / living body mass (g)].

[0155] 2.7 Determination of serum biochemical indicators.

[0156] After the experiment, 5 goslings were selected from each group, blood was collected from the heart, and the blood was separated by centrifugation at 3000 rpm for 15 min after standing at room temperature for 30 min. The serum was stored at -20°C, and the serum biochemical indicators total protein (TP), albumin (ALB), globulin (GLB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CRE), urea nitrogen (BUN), and uric acid (UA) were detected by biochemical analyzer.

[0157] 2.8 Enzyme-linked immunosorbent assay was used to detect inflammatory factors and immunoglobulins in the serum of goslings.

[0158] Blood was collected from the goslings by heart, and the blood was centrifuged at 3000 rpm for 15 min after standing at room temperature for 30 min to separate serum. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor α (TNF-α), interferon γ (IFN-γ), interleukin 1β (IL-1β), interleukin 6 (IL-6), immunoglobulin G (IgG), inducible nitric oxide synthase (iNOS), and xanthine oxidase (XOD) in serum.

[0159] 2.9 Viral load determination.

[0160] After the experiment, 5 goslings were selected from each group and killed, and their livers, kidneys, and feces were collected and stored in a -20°C refrigerator for later use. Total RNA was extracted for qRT-PCR detection.

[0161] 2.10 Data analysis.

[0162] The experimental results are expressed as mean ± standard deviation (Mean ± SD). Analysis of variance (ANOVA), significance of difference (Duncan multiple comparison method) and graphing were all performed using Prism 8.0.2. P < 0.05 indicated a significant difference, and P < 0.01 indicated a significant difference.

[0163] 3.Test results.

[0164] 3.1 Clinical observation

[0165] The goslings in the challenge group began to show clinical symptoms and died on the third day, mainly depression and white loose feces. The peak period of death was from the third to the ninth day after the challenge, with a total of 15 deaths and a mortality rate of 50%. Figure 7 and Figure 8 As shown, Figure 7 This is a comparison chart of the effects of different treatment groups on the heart and liver of goslings. Specifically, the blank control group (A) had normal heart and liver; the model group (B) had urate deposition in the heart and liver; the hearts and livers of all components in the Chinese medicine prevention group and the Chinese medicine treatment group were normal. Figure 8 The following is a comparison chart of the effects of different treatment groups on the kidneys of goslings. Specifically, the blank control group (A) had normal kidneys; the model group (B) had enlarged and mottled kidneys, with white urate deposits in the ureters on both sides; the Chinese medicine prevention group and the Chinese medicine treatment group (C1 and D1) administered according to formula ① had slight bleeding and swelling in the kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C2 and D2) administered according to formula ② had slight bleeding and swelling in the kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C3-H and D3-H) administered with a high dose of formula ③ had slight bleeding in the kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C3-M and D3-M) administered with a medium dose of formula ③ had normal kidneys; the Chinese medicine prevention group and the Chinese medicine treatment group (C3-L and D3-L) administered with a low dose of formula ③ had slight swelling in the kidneys.

[0166] It can be seen that the model group had obvious urate deposits on the surface of the heart and liver, enlarged kidneys with bleeding spots, and white urate deposits on both ureters, while the control group had no lesions in all organs after autopsy. With the feeding of Chinese medicine, the goslings in the Chinese medicine prevention group and treatment group were in better spirits than the virus control group, and the degree of kidney damage was lower than that of the virus control group.

[0167] 3.2 Effect of Chinese medicine prescription on the production performance of goslings.

[0168] Table 10 Effects of different treatment groups on the mortality of goslings

[0169]

[0170]

[0171] The effects of different treatment groups on the mortality of goslings are shown in Table 10. It can be seen from Table 10 that, compared with Group B, the medium-dose group of Formulation ③ is the best group in terms of both prevention and treatment, and has a significant protective effect against goose astrovirus, and can significantly reduce the mortality of goslings caused by astrovirus infection, with a protection rate of 93.33%.

[0172] Table 11 Effects of different treatment groups on the growth performance of goslings

[0173]

[0174] Note: * indicates comparison with group B, P < 0.05.

[0175] As shown in Table 11, compared with the model group, the average daily weight gain and final body weight of goslings in the medium-dose group of formula ③ were significantly increased (P < 0.05), and the feed-to-weight ratio was significantly reduced (P < 0.05).

[0176] 3.3 Effects of Chinese medicine prescriptions on organ indexes and intestines of goslings.

[0177] The liver is one of the important immune organs, which contains a large number of immune cells and immune-related molecules. The spleen is an important peripheral immune organ and the site of humoral and cellular immune responses. The bursa of Fabricius is a unique central immune organ of poultry, which can produce B lymphocytes and thus produce specific antibodies to complete specific immune responses. The intestine is not only the main site for digestion and absorption, but also the largest immune organ in the body. The measurement of intestinal length and morphology can reflect the digestion and absorption capacity of the intestine and the mucosal immune function.

[0178] As shown in Table 12, compared with the model group, the bursa index of the goslings in the Chinese medicine formula group (C3) was increased, among which the medium-dose group was significantly increased (P < 0.05), the spleen index of each group was reduced but the difference was not significant, the liver index was not significantly different, and the intestinal length was significantly increased (P < 0.05). The above results show that the medium-dose group of the Chinese medicine formula can enhance the immunity of goslings.

[0179] Table 12 Effects of different treatment groups on gosling organ indexes and intestinal

[0180]

[0181]

[0182] Note: * indicates comparison with group B, P < 0.05.

[0183] 3.4 Effect of Chinese medicine prescription on serum liver function of goslings.

[0184] The contents of serum liver function indicators are shown in Table 13. Compared with group A, there were no significant differences in the total protein, albumin, and globulin contents in the serum of goslings in each group (P>0.05). Compared with group A, the ALT and AST contents of model group B increased significantly (P<0.05); compared with group B, the drug test groups all decreased to varying degrees, among which the Chinese medicine formula treatment group (D3-M) had a more significant effect. The above results show that gout causes damage to the liver of goslings, and the Chinese medicine experimental group can effectively correct this damage and maintain the health of the liver.

[0185] Table 13 Effects of different treatment groups on serum liver function in goslings

[0186] Group TP(g / L) ALB(g / L) GLB(g / L) ALT(U / L) AST(U / L) A 39.58±4.83 16.66±1.42 22.92±3.66 17.30±3.62 46.46±6.17 B 53.36±12.67 20.20±2.36 33.16±10.38 <![CDATA[37.34±6.74 *** ]]> <![CDATA[84.89±8.67 * ]]> C1 52.94±9.16 20.92±2.93 32.02±6.32 <![CDATA[20.75±6.71 ## ]]> 55.70±17.82 C2 56.84±12.30 20.90±4.10 35.94±8.50 24.83±5.99 57.18±11.62 C3-M 45.20±5.92 18.18±1.85 27.02±4.21 <![CDATA[17.78±6.30 ### ]]> 53.27±14.79 D1 44.24±10.13 18.14±4.42 26.10±5.95 <![CDATA[22.09±5.80 # ]]> 51.05±22.31 D2 47.18±6.72 18.80±2.84 28.38±4.02 <![CDATA[20.18±2.54 ## ]]> 56.56±9.52 D3-M 47.66±12.47 18.82±4.73 28.82±7.83 <![CDATA[16.76±5.27 ### ]]> <![CDATA[45.51±9.02 # ]]>

[0187] Note: * indicates that compared with group A, P < 0.05; # indicates that compared with group B, P < 0.05

[0188] 3.5 Effect of Chinese medicine on serum renal function of gout goslings.

[0189] The contents of serum renal function indexes are shown in Table 14. Compared with group A, the serum creatinine, urea nitrogen and uric acid contents of group B increased significantly, while compared with group B, the drug test groups all decreased to varying degrees, among which the effect of group D3-M was more significant. The above results show that gout causes damage to the kidneys of goslings, and the Chinese medicine experimental group can effectively reduce this damage and maintain the health of the kidneys.

[0190] Table 14 Effects of different treatment groups on serum renal function of goslings

[0191]

[0192]

[0193] Note: * indicates that compared with group A, P < 0.05; # indicates that compared with group B, P < 0.05

[0194] 3.6 Effect of traditional Chinese medicine on serum XOD.

[0195] XOD is mainly distributed in the liver and is a key enzyme in the purine metabolic pathway. It catalyzes the oxidation of hypoxanthine to produce xanthine and superoxide anions, and further oxidizes xanthine to uric acid. In addition, when liver function is impaired, XOD will be released into the serum in large quantities. Therefore, the increase in serum XOD activity can sensitively reflect acute liver damage.

[0196] As shown in Table 15, compared with group A, the serum XOD content of group B increased significantly, while compared with group B, the drug test groups all decreased to varying degrees, among which the D3-M group had a more significant effect. The above results show that different drug test groups have alleviated the damage caused by gout to a certain extent, among which the D3-M group has the most significant effect.

[0197] Table 15 Effects of different treatment groups on serum XOD in goslings

[0198] Group XOD(pg / mL) A 8.55±0.91 B 28.66±1.03*** C1 23.35±0.74### C2 24.18±1.25### C3-M 16.38±1.22### D1 23.76±0.70### D2 23.87±0.98### D3-M 15.74±0.84###

[0199] Note: * indicates that compared with group A, P < 0.05; # indicates that compared with group B, P < 0.05

[0200] 3.7 Effect of Chinese medicine prescriptions on serum inflammatory factors.

[0201] The serum inflammatory factor contents are shown in Table 16. Compared with group A, the serum inflammatory factor contents in group B were significantly increased, while compared with group B, the drug intervention groups all decreased to varying degrees, among which the effects of C3-M and D3-M groups were more significant, indicating that the Chinese medicine significantly reduced the inflammatory response of goslings.

[0202] Table 16 Effects of different treatment groups on serum inflammatory factors in goslings

[0203]

[0204]

[0205] Note: * indicates that compared with group A, P < 0.05; # indicates that compared with group B, P < 0.05

[0206] 3.8 Effect of Chinese medicine prescription on viral load of goslings.

[0207] The results of the viral loads in the kidneys, livers, and feces of the different treatment groups after inoculation are shown in Tables 17 and Fig. 9 As shown in the figure, the virus can still be detected in the goslings 14 days after the virus attack, and the organ with the highest viral load is the kidney, among which kidney>liver>feces. Compared with the virus attack group, the Chinese medicine formula intervention group (medium dose) can significantly reduce the viral load of the kidney and liver, and the viral load of feces also decreases to varying degrees, but the difference is not significant.

[0208] Table 17 Viral loads in kidney, liver and feces of different treatment groups after inoculation (copies / μL)

[0209] Group <![CDATA[Kidney (log 10 )]]> <![CDATA[Liver (log 10 )]]> <![CDATA[Feces (log 10 )]]> A. Blank control group - - - B. Virus-challenged model group 5.28 4.3 3.86 C1 Tongue Prevention Group 4.29 3.59 3.28 C2 Sini Decoction prevention group 4.27 3.3 3.22 C3-M Chinese medicine prevention group 4.03 3.14 3.19 D1 Tongfengling treatment group 4.4 3.54 3.55 D2 Sini Decoction treatment group 4.47 3.61 3.5 D3-M Chinese medicine treatment group 4.29 3.28 3.26

[0210] 3.9 Effect of traditional Chinese medicine on serum IgG.

[0211] The serum IgG levels are shown in Table 18. Compared with group A, the serum IgG levels in group B were significantly increased, while compared with group B, the drug intervention groups (prevention group and treatment group) all decreased to varying degrees, among which the D3-M group and C3-M group were more significant. The serum IgG level was positively correlated with the virus content in the body.

[0212] Table 18 Effects of different treatment groups on serum IgG

[0213] Group IgG (pg / mL) A 13.20±1.52 B 35.87±1.06*** C1 31.24±1.29### C2 31.02±0.84### C3-M 22.20±0.90### D1 30.72±1.18### D2 30.84±1.11### D3-M 22.66±0.79###

[0214] Note: * indicates that compared with group A, P < 0.05; # indicates that compared with group B, P < 0.05.

[0215] In summary, compared with the model group, the Chinese medicine formula (Plantago, Radix Isatidis, Radix Astragali, Radix Saposhnikoviae) of the present invention can significantly reduce the mortality of goslings, and reduce the mortality of goslings caused by astrovirus infection from 50% to 6.67%, while the mortality of the positive drug group (C1 Tongfengling) only decreased from 50% to 26.67%. It can be seen that compared with the positive drug, the Chinese medicine formula of the present invention can significantly reduce the mortality of goslings. In addition, the formula can also significantly improve the growth performance and immunity of goslings.

[0216] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creative work, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating gout in goslings, characterized in that: The traditional Chinese medicine composition comprises the following raw materials for preparation: plantago seed, isatis root, astragalus root and siler.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition comprises the following raw materials in parts by weight: 1-10 parts of Plantago seeds, 1-10 parts of Isatis root, 1-10 parts of Astragalus and 1-10 parts of Saposhnikovia divaricata.

3. A Chinese medicine preparation for preventing and treating gout in goslings, characterized in that: The raw materials for preparing the Chinese medicine preparation include the Chinese medicine composition according to claim 1 or 2 and auxiliary materials acceptable in Chinese medicine.

4. The Chinese medicine preparation according to claim 3, characterized in that: When the dosage form of the Chinese medicine preparation is a decoction, each milliliter of the Chinese medicine preparation contains 1 g of the raw drug.

5. The method for preparing the Chinese medicine preparation according to claim 3 or 4, comprising the following steps: Mixing the raw materials and water, boiling and filtering to obtain a filter residue and a first filtrate; Mix the filter residue with water, boil and filter to obtain a second filtrate; The first filtrate and the second filtrate are combined and concentrated to obtain the traditional Chinese medicine preparation.

6. The preparation method according to claim 5, characterized in that: The concentration includes concentration under reduced pressure.

7. The preparation method according to claim 5, characterized in that: The mass ratio of the raw material to water is 1:(5-10); the mass ratio of the filter residue to water is 1:(5-10); The boiling time ranges from 0.5 to 2 hours.

8. The preparation method according to claim 5, characterized in that: The raw materials for preparation also include soaking before boiling; The soaking time ranges from 0.5 to 1 hour.

9. A feed having the function of preventing and treating gout in goslings, characterized in that: The ingredients of the feed include the Chinese medicine composition according to claim 1 or 2, the Chinese medicine preparation according to claim 3 or 4, or the Chinese medicine preparation prepared by the preparation method according to any one of claims 5 to 8 and a basic daily diet.

10. Use of the Chinese medicine composition according to claim 1 or 2, or the Chinese medicine preparation according to claim 3 or 4, or the Chinese medicine preparation prepared by the preparation method according to any one of claims 5 to 8, or the feed according to claim 9 in any one of the effects ① to ③: ① Reduce the mortality of goslings caused by goose astrovirus; ② Improve the growth performance of goslings; ③ Enhance the immunity of goslings.

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