Preview imaging method and device for transparent fluorescent sample

Abstract

The invention discloses a preview imaging method, device and equipment for a transparent fluorescent sample, and the method comprises the steps: carrying out the shooting of a transparent fluorescent slice, and obtaining a transparent preview; switching a fluorescent light filter or a light source until a plurality of fluorescent channels for developing the transparent fluorescent section are obtained; determining a preview scanning area according to a sample position range in the transparent preview, selecting a target fluorescent channel from a plurality of fluorescent channels to perform coverage scanning on the transparent preview based on the determined preview scanning area to obtain a plurality of view images, and splicing the plurality of view images to generate a large preview scanning image; zooming the large preview scanning image to be the same as the preview scanning area in size, and replacing a corresponding area in the transparent preview image with the zoomed large preview scanning image to obtain a replaced preview scanning image; and identifying and framing a sample scanning area in the preview scanning image to obtain a target preview image. A sample scanning area can be clearly identified and framed, and the accuracy and efficiency of scanning are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical image processing, and in particular to a preview imaging method and device for a transparent fluorescent sample. Background Art

[0002] For fluorescent slides, there are a lot of transparent samples, which makes it very difficult to frame the scan area through the preview image. Transparent fluorescent samples usually refer to biological samples observed under a fluorescence microscope. These samples have been treated to make them fluoresce under certain wavelengths of light while maintaining transparency. This transparency is usually achieved by removing or reducing light scattering and absorption in the sample.

[0003] At present, it is usually necessary to add an illumination light path on the side of the slice (such as Figure 1 As shown in the figure, a light source of a specific wavelength is incident, and the sliced ​​glass carrier is used as a light guide to allow the light to penetrate and reach the sample area. Since this is a fluorescent sample, it will be excited at a specific wavelength, thereby emitting light of another wavelength, which can be captured by the human eye or the preview camera, thus making the transparent and invisible sample appear visible ( Figure 1 The blue part in the black frame) can be used to accurately frame the scan area.

[0004] However, this transparent sample preview solution requires additional modifications to the slice clamp in order to accommodate the newly added light source structure. A set of LED lamp beads needs to be added and a matching control circuit is required, which increases the cost. In addition, the modified slice clamp is not compatible with normal slices, which also increases the cost of manufacturing and use. In addition, the light source enters from the side, while the preview camera takes pictures from above the slice, the light direction is inconsistent, and the imaging effect is not clear enough. Summary of the invention

[0005] In view of this, the purpose of the present invention is to propose a preview imaging method, device and equipment for transparent fluorescent samples, aiming to solve how to improve the recognition accuracy of the transparent sample area in the fluorescent slice preview image without increasing additional costs and modifying the slice clamp, so as to accurately frame the scanning area while ensuring the clarity of the imaging effect.

[0006] To achieve the above object, the present invention provides a preview imaging method for a transparent fluorescent sample, the method comprising:

[0007] Photograph the transparent fluorescent slice to obtain a transparent preview image;

[0008] By switching the fluorescence filter or light source until multiple fluorescence channels are obtained for developing the transparent fluorescent slice;

[0009] Determine a preview scanning area according to the sample position range in the transparent preview image, select a target fluorescent channel from multiple fluorescent channels based on the determined preview scanning area to cover scan the transparent preview image, obtain multiple field of view images, and splice the multiple field of view images to generate a large preview scan image;

[0010] Scaling the preview scanned large image to the same size as the preview scanned area, and replacing the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image;

[0011] The sample scan area in the preview scan image is identified and framed to obtain a target preview image.

[0012] Preferably, based on the determined preview scanning area, selecting a target fluorescent channel from a plurality of fluorescent channels to perform a coverage scan on the transparent preview image includes:

[0013] The transparent preview image is preview-scanned by using a physical low-power objective lens or a virtual low-power objective lens.

[0014] Preferably, the step of performing a preview scan on the transparent preview image by using a physical low-power objective lens or a virtual low-power objective lens comprises:

[0015] When the scanner is equipped with a solid low-power objective lens, the transparent preview image is preview-scanned using the corresponding solid low-power objective lens;

[0016] When the scanner is not equipped with a solid low-magnification objective lens, the scanning step length of the high-magnification objective lens with a preset magnification is adjusted by modifying the configuration, and the transparent preview image is scanned by the obtained virtual low-magnification objective lens.

[0017] Preferably, the step of identifying and framing the sample scan area in the preview scan image to obtain a target preview image includes:

[0018] Automatically identifying the sample scan area in the preview scan image using an image processing algorithm to obtain a recognition result;

[0019] According to the recognition result, a sample area is framed on the preview scan image to generate a target preview image.

[0020] Preferably, the automatically identifying the sample scanning area in the preview scanning image by using an image processing algorithm comprises:

[0021] The sample scanning area in the preview scanning image is identified using any one of an edge contour detection algorithm, an image segmentation algorithm, and an image recognition algorithm based on deep learning.

[0022] Preferably, the step of stitching a plurality of the field of view images to generate a large preview scan image includes:

[0023] A plurality of the field of view images are stitched together using a seamless image stitching algorithm to generate a large preview scan image.

[0024] To achieve the above object, the present invention further provides a preview imaging device for a transparent fluorescent sample, the device comprising:

[0025] A slice shooting unit is used to shoot transparent fluorescent slices to obtain a transparent preview image;

[0026] A channel switching unit, used for switching fluorescent filters or light sources until a plurality of fluorescent channels for developing transparent fluorescent slices are obtained;

[0027] A preview scanning unit, configured to determine a preview scanning area according to a sample position range in the transparent preview image, select a target fluorescent channel from a plurality of fluorescent channels based on the determined preview scanning area to perform a cover scan on the transparent preview image, obtain a plurality of field of view images, and splice the plurality of field of view images to generate a large preview scan image;

[0028] An image replacement unit is used to scale the preview scanned large image to the same size as the preview scanned area, and replace the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image;

[0029] The area recognition unit is used to recognize and frame the sample scanning area in the preview scanning image to obtain a target preview image.

[0030] In order to achieve the above objectives, the present invention also proposes a preview imaging device for transparent fluorescent samples, including a fluorescent microscope and scanning software stored in the fluorescent microscope; the device is used to implement the steps of a preview imaging method for transparent fluorescent samples as described in the above embodiment.

[0031] Beneficial effects:

[0032] The above scheme, by shooting transparent fluorescent slices directly below the preview camera of the scanner, can ensure that the direction of light is consistent with the shooting direction of the preview camera, thereby improving the clarity and accuracy of the imaging; by switching the fluorescent filter or light source, the development images of multiple fluorescent channels can be obtained, which helps to more comprehensively understand the fluorescence characteristics of the sample. By first shooting a transparent preview image and then performing an overlay scan based on the preview scan area, the scanning area of ​​the sample can be quickly determined, avoiding unnecessary scanning and data processing. By replacing and scaling the preview scan image, the sample scanning area can be clearly identified and framed, improving the accuracy and efficiency of the scan. Preview imaging of transparent fluorescent samples can be achieved without making additional changes to the scanner to facilitate framing the scanning area. In addition, since there is no need to modify the slice clamp, this method can be applied to various models of scanners and slice clamps, improving the versatility and compatibility of the equipment.

[0033] The above scheme, by using a physical low-magnification objective or a virtual low-magnification objective for preview scanning, can achieve a large-scale, low-resolution rapid preview of transparent fluorescent samples without adding additional hardware costs. This helps to quickly determine the scanning area of ​​the sample and provide guidance for subsequent high-resolution scanning.

[0034] The above scheme, by using a physical low-magnification objective lens or a virtual low-magnification objective lens for preview scanning, can achieve a large-scale, low-resolution fast preview of transparent fluorescent samples without adding additional hardware costs. This helps to quickly determine the scanning area of ​​the sample and provide guidance for subsequent high-resolution scanning. In addition, it can flexibly adapt to different models of scanners, reducing the dependence on scanner hardware. At the same time, by modifying the configuration to adjust the scanning step size of the high-magnification objective lens, the function of the virtual low-magnification objective lens can be realized, further improving the versatility and applicability of the method.

[0035] The above scheme can realize fast and accurate identification of the sample area by automatically identifying the sample scanning area in the preview scan image using the image processing algorithm. This helps to reduce manual intervention and improve the degree of automation of scanning. In addition, an edge contour detection algorithm, an image segmentation algorithm, and an image recognition algorithm based on deep learning are provided to identify the sample scanning area, which can be flexibly adjusted according to the specific sample characteristics and scanning requirements, thereby realizing more accurate identification of the sample area.

[0036] The above scheme uses a seamless image stitching algorithm to stitch multiple field of view images to generate a complete preview scan image, ensuring that the quality of the stitched image is not affected, which helps to achieve comprehensive observation and analysis of the sample and provide accurate image information for subsequent analysis and identification. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0038] Figure 1 A preview schematic diagram of adding an illumination light path to the side of a slice provided as background technology of the present invention.

[0039] Figure 2 A schematic flow chart of a preview imaging method for a transparent fluorescent sample provided by an embodiment of the present invention.

[0040] Figure 3 This is a transparent preview image obtained by shooting provided by an embodiment of the present invention.

[0041] Figure 4 A plurality of field of view images obtained by scanning a preview scanning area provided by an embodiment of the present invention.

[0042] Figure 5 A schematic diagram of an image scanned by a virtual low-magnification objective lens provided by an embodiment of the present invention.

[0043] Figure 6 A preview scan image after replacement provided by an embodiment of the present invention.

[0044] Figure 7 A schematic structural diagram of a preview imaging device for a transparent fluorescent sample provided by an embodiment of the present invention.

[0045] The realization of the purpose, functional features and advantages of the invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0047] The content of the present invention is described in detail below with reference to embodiments.

[0048] Reference Figure 2 FIG. 1 is a flow chart of a preview imaging method of a transparent fluorescent sample provided by an embodiment of the present invention.

[0049] In this embodiment, the method includes:

[0050] S11, photographing the transparent fluorescent slice to obtain a transparent preview image.

[0051] In this embodiment, before starting preview imaging, check the status of the scanner and preview camera to ensure that they are in normal working condition. Move the transparent fluorescent slice to the bottom of the preview camera, turn on the illuminator, and adjust the brightness and angle of the illuminator to obtain the best lighting effect, illuminating the sample area and the label area ( Figure 3 The transparent fluorescent slice (the left side of the middle is the transparent sample area, and the right side is the text label area) is controlled to take a picture of the transparent fluorescent slice to obtain a transparent preview image in which the sample area is not clearly visible.

[0052] S12, switching the fluorescent filter or the light source until a plurality of fluorescent channels for developing the transparent fluorescent slices are obtained.

[0053] In this embodiment, by switching the fluorescent filter or light source to the fluorescent channel that can be developed (DAPI / FITC / CY3 / CY5 / CY6, etc., DAPI / FITC / CY3 / CY5 / CY6 refers to different fluorescent dyes or fluorescent markers, each of which has a specific excitation and emission spectrum, and is commonly used in multi-channel fluorescence imaging of biological samples), different fluorescent channels are tested one by one until the best development effect is found. By adjusting the objective lens to a normal magnification (such as 20X) and adjusting the platform to a clear focal plane, ensure that the sample can be clearly imaged under the selected fluorescent channel; adjust the exposure and gain of the scanning camera to make the fluorescent signal bright enough. The characteristic of fluorescent samples is that under the excitation of the fluorescent light source, the emitted light is generated to develop the sample and present different colors, that is, different excitation colors are presented under different fluorescent channels during microscopic imaging.

[0054] S13, determining a preview scanning area according to the sample position range in the transparent preview image, and based on the determined preview scanning area, selecting a target fluorescent channel from multiple fluorescent channels to perform a cover scan on the transparent preview image to obtain multiple field of view images, and splicing the multiple field of view images to generate a large preview scan image.

[0055] Further, in step S13, based on the determined preview scanning area, a target fluorescent channel is selected from a plurality of fluorescent channels to perform a coverage scan on the transparent preview image, including:

[0056] The transparent preview image is preview-scanned by using a physical low-power objective lens or a virtual low-power objective lens.

[0057] The method of performing a preview scan on the transparent preview image by using a physical low-power objective lens or a virtual low-power objective lens includes:

[0058] When the scanner is equipped with a solid low-power objective lens, the transparent preview image is preview-scanned using the corresponding solid low-power objective lens;

[0059] When the scanner is not equipped with a solid low-magnification objective lens, the scanning step length of the high-magnification objective lens with a preset magnification is adjusted by modifying the configuration, and the transparent preview image is scanned by the obtained virtual low-magnification objective lens.

[0060] Furthermore, in step S13, the plurality of field of view images are stitched together to generate a large preview scan image, including:

[0061] A plurality of the field of view images are stitched together using a seamless image stitching algorithm to generate a large preview scan image.

[0062] In this embodiment, a larger preview scanning area is preselected on the transparent preview image to ensure that the sample area can be covered. A brighter channel (such as the green channel) is selected from multiple fluorescence channels as the target fluorescence channel, and the preview scanning area is covered and scanned to obtain multiple field of view images (refer to Figure 4 As shown in the figure). During microscopic scanning, the field of view under the objective lens is small, so multiple fields of view need to be scanned to cover the entire scanning area. Then, by using a seamless image stitching algorithm, the multiple small field of view images are stitched together (through the process of aligning the edge overlaps of the small field of view images to be stitched together based on image feature matching) to generate an overall preview scan image.

[0063] Since the exposure time of fluorescence imaging is long, the preview scan of the fluorescence slice will take a long time. Therefore, the preview scan speed is further improved by using a solid low-magnification objective lens (such as a 4X objective lens) or a virtual low-magnification objective lens (such as a virtual 10X objective lens). When the scanner is equipped with a solid low-magnification objective lens, use the solid low-magnification objective lens for preview scanning. The field of view of a solid low-magnification objective lens is inversely proportional to its magnification (for example, the field of view of a 4X objective lens is 25 times that of a 20X objective lens. 25 images need to be scanned under a 20X objective lens, while only 1 image needs to be scanned under a 4X objective lens), and the scanning speed is greatly improved.

[0064] When the scanner is not equipped with a physical low-magnification objective lens, a high-magnification objective lens (such as 20X) can be used to simulate a low-magnification objective lens (such as 10X) to increase the scanning speed. Although the actual 20X objective lens is used, the configuration is modified to make the software think that the 10X objective lens is now used, so the software will "sample" scan the sample with a step length twice that of the 20X objective lens. The so-called "sampling" means that the scanning field interval is discontinuous (discontinuous image acquisition does not affect the definition of the sample scanning area, and the scanning speed is at least doubled) (refer to Figure 5 As shown in the figure, this sampling method is feasible because only the rough outline of the sample scanning area is needed and the accuracy of the details is not important.

[0065] When a virtual low-magnification objective lens is used to preview and scan a transparent preview image, the scanning step length is automatically calculated and adjusted according to the magnification of the virtual low-magnification objective lens. That is, the X / Y step length of the interval sampling can be automatically calculated and adjusted as the magnification of the virtual objective lens changes, so as to ensure complete coverage of the sample scanning area and improve the scanning speed.

[0066] S14, scaling the preview scanned large image to the same size as the preview scanned area, and replacing the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image.

[0067] In this embodiment, the size of the preview scanned image is reduced to make it consistent with the size of the preview scanned area selected on the transparent preview image. The reduced preview scanned image is used to replace the same area in the transparent preview image to obtain a replaced preview scanned image (refer to Figure 6 ). Check the preview scan image after replacement to make sure the sample area is clearly visible. At this point, the sample that was previously invisible has been made visible under fluorescence, making it possible to frame the scan area.

[0068] S15, identifying and framing the sample scan area in the preview scan image to obtain a target preview image.

[0069] Furthermore, in step S15, the sample scan area in the preview scan image is identified and framed to obtain a target preview image, including:

[0070] Automatically identifying the sample scan area in the preview scan image using an image processing algorithm to obtain a recognition result;

[0071] According to the recognition result, a sample area is framed on the preview scan image to generate a target preview image.

[0072] The method of automatically identifying the sample scan area in the preview scan image by using an image processing algorithm includes:

[0073] The sample scanning area in the preview scanning image is identified using any one of an edge contour detection algorithm, an image segmentation algorithm, and an image recognition algorithm based on deep learning.

[0074] In this embodiment, for low-throughput scanners, the sample scanning area in the preview scan image is manually identified and framed. For high-throughput scanners, the sample scanning area needs to be automatically identified. An image processing algorithm for edge contour detection (such as Canny) can be used to identify the edge contour of the sample in the image; or an image segmentation technique (such as threshold segmentation, Otsu algorithm) can be used to distinguish between the sample and the background area to separate the sample from the background. It is also possible to train an AI convolutional neural network based on a deep learning image recognition algorithm for image recognition and segmentation (such as UNet or Mask RCNN). After the sample scanning area is identified and framed, you can add focus points and perform formal slice scanning according to the normal process.

[0075] Reference Figure 7 FIG. 1 is a schematic structural diagram of a preview imaging device for a transparent fluorescent sample provided by an embodiment of the present invention.

[0076] In this embodiment, the device 20 includes:

[0077] The slice shooting unit 21 is used to shoot the transparent fluorescent slice to obtain a transparent preview image;

[0078] A channel switching unit 22, used to switch fluorescent filters or light sources until multiple fluorescent channels for developing transparent fluorescent slices are obtained;

[0079] A preview scanning unit 23 is used to determine a preview scanning area according to the sample position range in the transparent preview image, select a target fluorescent channel from multiple fluorescent channels based on the determined preview scanning area to cover scan the transparent preview image, obtain multiple field of view images, and splice the multiple field of view images to generate a preview scanning large image;

[0080] The image replacement unit 24 is used to scale the preview scanned large image to the same size as the preview scanned area, and replace the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image;

[0081] The region recognition unit 25 is used to recognize and frame the sample scan region in the preview scan image to obtain a target preview image.

[0082] Each unit module of the device 20 can respectively execute the corresponding steps in the above method embodiment, so each unit module will not be described in detail here. Please refer to the description of the above corresponding steps for details.

[0083] The embodiment of the present invention also provides a preview imaging device for transparent fluorescent samples, including a fluorescent microscope and scanning software stored in the fluorescent microscope; correspondingly, the device can execute Figure 2 The technical solution of the method embodiment shown has similar implementation principles and technical effects. For details, please refer to the relevant records in the above embodiments, which will not be repeated here.

[0084] The device may include components such as a memory and a processor. The memory may be used to store software programs and modules, and the processor executes various functional applications and data processing by running the software programs and modules stored in the memory. The memory may mainly include a program storage area and a data storage area, wherein the program storage area may store an operating system, an application required for at least one function, etc.; the data storage area may store data created according to the use of the device, etc. In addition, the memory may include a high-speed random access memory, and may also include a non-volatile memory, such as at least one disk storage device, a flash memory device, or other volatile solid-state storage devices. Accordingly, the memory may also include a memory controller to provide the processor and the input unit with access to the memory.

[0085] It should be noted that each embodiment in this specification is described in a progressive manner, and each embodiment focuses on the differences from other embodiments, and the same or similar parts between the embodiments can be referred to each other. For the device embodiment or equipment embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0086] Furthermore, in this document, the terms "comprises," "comprising," or any other variation thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or device that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article, or device that includes the element.

[0087] The above description shows and describes the preferred embodiments of the present invention. It should be understood that the present invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the invention, through the above teachings or the technology or knowledge of the relevant field. The changes and modifications made by those skilled in the art shall not depart from the spirit and scope of the present invention, and shall be within the scope of protection of the claims attached to the present invention.

Claims

1. A preview imaging method for a transparent fluorescent sample, characterized in that: The method comprises: Photograph the transparent fluorescent slice to obtain a transparent preview image; By switching the fluorescence filter or light source until multiple fluorescence channels are obtained for developing the transparent fluorescent slice; Determine a preview scanning area according to the sample position range in the transparent preview image, select a target fluorescent channel from multiple fluorescent channels based on the determined preview scanning area to cover scan the transparent preview image, obtain multiple field of view images, and splice the multiple field of view images to generate a large preview scan image; Scaling the preview scanned large image to the same size as the preview scanned area, and replacing the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image; The sample scan area in the preview scan image is identified and framed to obtain a target preview image.

2. The preview imaging method of a transparent fluorescent sample according to claim 1, characterized in that: The step of selecting a target fluorescent channel from a plurality of fluorescent channels based on the determined preview scanning area to cover scan the transparent preview image includes: The transparent preview image is preview-scanned by using a physical low-power objective lens or a virtual low-power objective lens.

3. The preview imaging method of a transparent fluorescent sample according to claim 2, characterized in that: The method of performing a preview scan on the transparent preview image by using a physical low-power objective lens or a virtual low-power objective lens comprises: When the scanner is equipped with a solid low-power objective lens, the transparent preview image is preview-scanned using the corresponding solid low-power objective lens; When the scanner is not equipped with a solid low-magnification objective lens, the scanning step length of the high-magnification objective lens with a preset magnification is adjusted by modifying the configuration, and the transparent preview image is scanned by the obtained virtual low-magnification objective lens.

4. The preview imaging method of a transparent fluorescent sample according to claim 1, characterized in that: The step of identifying and framing the sample scan area in the preview scan image to obtain a target preview image includes: Automatically identifying the sample scan area in the preview scan image using an image processing algorithm to obtain a recognition result; According to the recognition result, a sample area is framed on the preview scan image to generate a target preview image.

5. The preview imaging method of a transparent fluorescent sample according to claim 4, characterized in that: The method of automatically identifying the sample scanning area in the preview scanning image by using an image processing algorithm includes: The sample scanning area in the preview scanning image is identified using any one of an edge contour detection algorithm, an image segmentation algorithm, and an image recognition algorithm based on deep learning.

6. The preview imaging method of a transparent fluorescent sample according to claim 1, characterized in that: The step of stitching the plurality of field of view images to generate a large preview scan image includes: A plurality of the field of view images are stitched together using a seamless image stitching algorithm to generate a large preview scan image.

7. A preview imaging device for transparent fluorescent samples, characterized in that: The device comprises: A slice shooting unit is used to shoot transparent fluorescent slices to obtain a transparent preview image; A channel switching unit, used for switching fluorescent filters or light sources until a plurality of fluorescent channels for developing transparent fluorescent slices are obtained; A preview scanning unit, configured to determine a preview scanning area according to a sample position range in the transparent preview image, select a target fluorescent channel from a plurality of fluorescent channels based on the determined preview scanning area to perform a cover scan on the transparent preview image, obtain a plurality of field of view images, and splice the plurality of field of view images to generate a large preview scan image; An image replacement unit is used to scale the preview scanned large image to the same size as the preview scanned area, and replace the corresponding area in the transparent preview image with the scaled preview scanned large image to obtain a replaced preview scanned image; The area recognition unit is used to recognize and frame the sample scanning area in the preview scanning image to obtain a target preview image.

8. A preview imaging device for transparent fluorescent samples, characterized in that: The device comprises a fluorescence microscope and scanning software stored in the fluorescence microscope; the device is used to implement the steps of a preview imaging method for a transparent fluorescent sample as described in any one of claims 1 to 6.

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