Composition for loosening bowel to relieve constipation as well as preparation method and application thereof

Abstract

The invention provides an intestine moistening and bowel relaxing composition as well as a preparation method and application thereof. The intestine moistening and bowel relaxing composition is prepared from the following raw materials in parts by weight: 5 to 10 parts of compound fruit and vegetable fermentation liquor, 2 to 4 parts of compound fruit concentrated juice, 0.01 to 0.02 part of compound probiotic powder, 17 to 27 parts of prebiotics, 0.1 to 0.15 part of DL-malic acid, 0.05 to 0.1 part of citric acid, 0.005 to 0.01 part of sodium citrate, 0.01 to 0.02 part of potassium sorbate and 59 to 65 parts of water. The preparation method comprises the following steps: S1, preparing a mixed solution A; s2, preparing a mixed solution B; s3, preparing a mixed solution C; s4, preparing composite fruit and vegetable fermentation liquor; s5, mixing; and the prepared composition for relaxing bowel can be applied to products for relaxing bowel, and has the effects of cleaning intestinal waste, relaxing bowel and regulating intestinal flora.

Description

Technical Field

[0001] The invention relates to an intestinal moistening and laxative composition and a preparation method and application thereof, belonging to the technical field of functional foods. Background Art

[0002] During the metabolism process, if harmful substances discharged by the human body cannot be "cleaned up" in time, it is easy to cause a decline in human body functions and induce diseases. For occasional constipation and constipation in bedridden people, some measures to assist defecation can be used, such as: using enema, glycerin suppository, etc. to lubricate the anus to assist in the discharge of feces, and even stimulant laxatives can be used to treat acute constipation and perform intestinal cleansing. However, these measures to assist defecation are not recommended for long-term use by patients. In particular, the main ingredients of many laxatives are rhubarb, senna leaves, etc., which can cause colitis, and patients are not recommended to take them for a long time.

[0003] In fact, some fruits, vegetables and plants also have certain effects of clearing intestinal waste and moistening the intestines. Therefore, the present invention is based on this, through research on various fruits, vegetables and plants, and selects burdock root, cassia seed, licorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, monk fruit, etc. as main raw materials, and through biological fermentation technology, develops a composition with the functions of clearing intestinal waste and moistening the intestines. Summary of the invention

[0004] In view of the above existing technical problems, the present invention provides a laxative composition and a preparation method and application thereof, so as to achieve the technical purposes of cleaning intestinal waste, laxative and regulating intestinal flora.

[0005] To achieve the above-mentioned purpose, firstly, the present invention provides a laxative composition, comprising the following raw materials and their weight portions: 5-10 parts of composite fruit and vegetable fermentation liquid, 2-4 parts of composite fruit concentrated juice, 0.01-0.02 parts of composite probiotic powder, 17-27 parts of prebiotics, 0.1-0.15 parts of DL-malic acid, 0.05-0.1 parts of citric acid, 0.005-0.01 parts of sodium citrate, 0.01-0.02 parts of potassium sorbate, and 59-65 parts of water.

[0006] The composite fruit and vegetable fermentation liquid comprises the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of amomum villosum, 1.9 parts of sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 part of lipase, 0.07 part of cellulase, and 0.05 part of pectinase.

[0007] The composite fruit concentrated juice comprises the following raw materials and their weight portions: 50 parts of apple concentrated juice, 25 parts of strawberry concentrated juice, 12.5 parts of cranberry concentrated juice, and 12.5 parts of blueberry concentrated juice.

[0008] The composite probiotic powder comprises the following raw materials and their weight portions: 25 parts of Lactobacillus plantarum, 25 parts of Lactobacillus acidophilus, 25 parts of Bifidobacterium longum, and 25 parts of Lactobacillus rhamnosus.

[0009] The prebiotics include the following raw materials and their weight proportions: 8-12 parts of xylo-oligosaccharide, 3-5 parts of lactitol, 3-5 parts of L-arabinose, and 3-5 parts of sorbitol liquid.

[0010] In the above technical solution, various raw materials in the composite fruit and vegetable fermentation liquid respectively achieve the effects of cleaning intestinal waste and moistening the intestine and relieving constipation through different mechanisms, which are specifically introduced as follows.

[0011] (1) Burdock root: It has the functions of clearing away heat and detoxifying, removing moisture from the body, strengthening the spleen and stimulating appetite, clearing the body's metabolic products, and detoxifying and relieving constipation.

[0012] (2) Cassia seed: It is sweet, bitter, salty and slightly cold in nature. It enters the liver, kidney and large intestine meridians. It has the effects of clearing the liver and improving eyesight, and moistening the intestines and relieving constipation.

[0013] (3) Licorice: It tastes sweet and is neutral in nature. It enters the heart, lung, spleen, and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, removing phlegm and relieving cough, relieving pain, and harmonizing various medicines. It can be used to relieve spleen and stomach weakness.

[0014] (4) Apricot kernel: It tastes bitter and is slightly warm in nature. It enters the lung and large intestine meridians. It has the effects of lowering qi, relieving cough and asthma, and moistening the intestines and relieving constipation. It can be used to treat cough and asthma, chest fullness and phlegm, and dry intestines and constipation.

[0015] (5) Hemp seed: It tastes sweet and is neutral in nature. It enters the spleen, stomach, and large intestine meridians. It has the effect of moistening the intestines and relieving constipation. It can be used to treat blood deficiency, body fluid deficiency, and dry intestines and constipation.

[0016] (6) Honeysuckle: It tastes sweet and is cold in nature. It enters the lung, heart, and stomach meridians. It has the effects of clearing away heat, detoxifying, and dispersing wind-heat.

[0017] (7) Houttuynia cordata: Spicy in taste, slightly cold in nature, enters the lung meridian, has the effects of clearing away heat and detoxifying, eliminating carbuncle and draining pus, and promoting diuresis and relieving stranguria.

[0018] (8) Amomum villosum: Spicy in taste, warm in nature, enters the spleen, stomach, and kidney meridians. It has the effects of removing dampness and stimulating appetite, warming the spleen and stopping diarrhea, regulating qi and stabilizing pregnancy.

[0019] (9) Sterculia lychnophora: sweet in taste, cold in nature, enters the lung and large intestine meridians, has the effects of clearing heat and moistening the lungs, relieving sore throat and opening voice, and moistening the intestines and relieving constipation.

[0020] (10) Perilla seeds: spicy in taste, warm in nature, and enter the lung meridian. They have the effects of lowering qi and resolving phlegm, relieving cough and asthma, and moistening the intestines and relieving constipation. They can be used to treat dry intestines and constipation.

[0021] (10) Dandelion: It tastes bitter and sweet, is cold in nature, and enters the liver and stomach meridians. It has the effects of clearing away heat and detoxifying, reducing swelling and dispersing nodules, and promoting diuresis and relieving stranguria.

[0022] (12) Portulaca oleracea; it is sour in taste and cold in nature. It enters the liver and large intestine meridians. It has the effects of clearing away heat and detoxifying, cooling blood and stopping bleeding, and stopping dysentery. It can be used to treat heat-toxic bloody dysentery, carbuncles, furuncles, eczema, erysipelas, snake and insect bites, blood in the stool, hemorrhoids, and metrorrhagia.

[0023] (13) Jujube: Sweet in taste, warm in nature, it enters the spleen, stomach, and heart meridians. It has the effects of tonifying the middle qi, nourishing blood, and calming the mind.

[0024] (14) Hawthorn: It tastes sour and sweet, is slightly warm in nature, and enters the spleen, stomach, and liver meridians. It has the effects of helping digestion and strengthening the stomach, promoting qi and relieving blood stasis, and clearing turbidity and reducing fat.

[0025] (15) Luo Han Guo: It tastes sweet and is cool in nature. It enters the lung and large intestine meridians. It has the effects of clearing heat and moistening the lungs, relieving sore throat and opening the voice, and lubricating the intestines and facilitating bowel movements. It can be used to treat constipation caused by dry intestines.

[0026] Moreover, the beneficial effects of each raw material in the composite fruit and vegetable fermentation liquid on the human body after being made into a composite material by a certain proportion are incomparable to those of a single raw material. Specifically, the present invention is based on the theory of traditional Chinese medicine, and preferably selects burdock root, cassia seed, licorice, apricot kernel, hemp seed, honeysuckle, houttuynia, amomum, Sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and monk fruit as the main effective ingredients of the composite fruit and vegetable fermentation liquid, and further combines prebiotics, composite fruit concentrate, and composite probiotic powder, which can not only promote better absorption of effective ingredients, but also play a synergistic promotion effect in terms of moistening the intestines and relieving constipation and regulating intestinal flora.

[0027] In addition, the compound fruit concentrated juice is rich in cellulose, which can promote intestinal peristalsis and prevent constipation. The natural enzyme therein helps digestion and regulates the taste of the beverage.

[0028] The composite probiotic powder has the functions of improving and regulating the balance of intestinal flora, inhibiting the growth of harmful microorganisms in the intestine, and maintaining intestinal health.

[0029] The xylooligosaccharides, lactitol and L-arabinose in the prebiotics can promote the proliferation of bifidobacteria, improve intestinal flora imbalance, promote gastrointestinal motility, increase the wetness of feces, reduce intestinal pH value, inhibit the growth of harmful bacteria, and play a role in improving constipation; sorbitol has a certain high osmotic effect, which can increase the moisture in the intestine, thereby softening the stool and has the benefit of moisturizing the intestine and facilitating bowel movements.

[0030] It can be seen that the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder and prebiotics can promote each other and jointly achieve the technical objectives of cleaning intestinal waste, moistening the intestine and facilitating bowel movements, and regulating intestinal flora.

[0031] Secondly, the present invention provides a method for preparing the laxative composition, comprising the following steps: S1. Prepare mixed solution A: weigh enoki mushroom, carrot, yam, cucumber, broccoli, mulberry, apple, pear, grapefruit, pineapple, papaya, orange, peach, lemon, grape, blueberry, and strawberry respectively, wash them, mix them evenly, then add an equal amount of water to squeeze the juice, and then filter to obtain mixed solution A.

[0032] S2. preparing mixed solution B: weighing burdock root, cassia seed, liquorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and monk fruit respectively, mixing them evenly, then soaking them in water, boiling them, and filtering them to obtain mixed solution B.

[0033] The method of the present invention further comprises that in step S2, the soaking time is 0.5-1h.

[0034] In the above technical solution, each raw material is soaked for a period of time before boiling, so that the effective ingredients in the raw materials can be dissolved in the medicinal tissue. However, if the soaking time is too long, it is easy to cause the efficacy to decrease, so the soaking time is preferably selected to be 0.5-1h.

[0035] The method of the present invention further comprises: in step S2, the boiling temperature is 80-90° C., the boiling time is 1-2 hours, and the boiling is stopped when half of the added water remains.

[0036] S3. Prepare mixed solution C: Mix mixed solution A and mixed solution B evenly, and then sterilize under high pressure and high temperature to obtain mixed solution C.

[0037] In the method of the present invention, in step S3, the sterilization temperature is 121° C. and the sterilization time is 30 min.

[0038] In the above technical solution, high temperature instantaneous sterilization is used to obtain smaller molecular clusters, which can enhance the permeability, solubility and diffusion power of the prepared mixed solution C.

[0039] S4, preparing the composite fruit and vegetable fermentation liquid: first adding yeast to the mixed liquid C for the first fermentation, then adding lactic acid bacteria for the second fermentation, and then filtering and clarifying the fermentation liquid to obtain the composite fruit and vegetable fermentation liquid.

[0040] The method of the present invention further comprises: in step S4, when the yeast is fermented for the first time, 0.01 g of yeast is added to every 100 g of mixed solution C, the fermentation temperature is 20-30° C., and the fermentation time is 2-4 days.

[0041] In the above technical solution, since too high or too low fermentation temperature will hinder the reproduction and growth of yeast, it is appropriate to select a fermentation temperature of 20-30°C and a fermentation time of 2-4 days.

[0042] The method of the present invention is further characterized in that in step S4, when the yeast is undergoing the first fermentation, 0.1 g of lipase, 0.07 g of cellulase and 0.05 g of pectinase are added to every 100 g of the mixed solution C.

[0043] In the above technical solution, the role of lipase is to improve the digestibility and taste of oil. In the mixed liquid C, lipase can hydrolyze the ester bonds in the fat molecules to generate glycerol and fatty acids, which helps to improve the taste and flavor of the product.

[0044] The role of cellulase is to improve the taste and nutritional value of food. Through the action of cellulase, the cellulose in the mixed liquid C is partially hydrolyzed, making the texture of the mixed liquid C softer, thereby improving its taste and nutritional value.

[0045] The function of pectinase is to improve the texture and taste of fruit and vegetable products. Since pectinase can hydrolyze the glycosidic bonds in pectin molecules and reduce the viscosity of pectin, in mixed liquid C, pectinase partially hydrolyzes the pectin, making the texture softer and easier to chew.

[0046] Therefore, adding lipase, cellulase and pectinase during the fermentation process can, through specific enzymatic reactions, improve the digestibility and taste of oils, enhance the taste and nutritional value of plant foods, and improve the texture and taste of fruit and vegetable products, thereby optimizing the overall quality of the product and eating experience.

[0047] The method of the present invention further comprises: in step S4, when the lactic acid bacteria undergo a second fermentation, 0.015 g of lactic acid bacteria is added to every 100 g of mixed solution C, the fermentation temperature is 25-30° C., and the fermentation time is 48-72 h.

[0048] Furthermore, since too low pH will inhibit the growth of lactic acid bacteria, in step S4, when the lactic acid bacteria undergo a second fermentation, the fermentation is stopped when the pH of the fermentation liquid reaches 3-3.4.

[0049] In the above technical solution, yeast is used for the first fermentation and lactic acid bacteria is used for the second fermentation. The mixed liquid C is fermented in stages, which can improve the performance of the mixed liquid C and achieve the effect of cleaning intestinal waste and moisturizing the intestines.

[0050] S5. Mixing: Weigh the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder, prebiotics, DL-malic acid, citric acid, sodium citrate, potassium sorbate and water respectively, mix them evenly, and then sterilize and fill them to obtain a laxative composition product.

[0051] Finally, the present invention provides an application of the laxative composition in the preparation of a laxative product, in particular, the composition can be applied in a laxative beverage product.

[0052] In summary, the "three-in-one" in the laxative composition of the present invention refers to compound fruit and vegetable fermentation liquid, compound probiotic powder and prebiotics, which are specifically introduced as follows.

[0053] First, the compound fruit and vegetable fermentation liquid uses components that are both medicinal and edible, which achieve the effect of moisturizing the intestines and promoting bowel movements through different mechanisms. Through scientific compatibility, the synergistic effect between the drug components is significantly improved, the effect of moisturizing the intestines and promoting bowel movements is improved, and no toxic side effects or dependence are produced.

[0054] Secondly, compound probiotic powder can regulate intestinal health, accelerate the excretion of harmful substances and toxins in the intestines, and play a role in laxative and detoxification.

[0055] Thirdly, prebiotics are water-soluble dietary fibers that can effectively improve the intestinal environment, promote the growth of beneficial bacteria, and inhibit the reproduction of harmful bacteria, thereby achieving a light and smooth effect. In particular, L-arabinose is a low-GI compound sugar that has the effect of reducing weight and lowering blood sugar, and is more friendly to obese and diabetic people.

[0056] In addition, the raw material source of the invention is wide, the preparation cost is low, it is safe and has no side effects, and it has broad application prospects in the preparation of intestinal moisturizing and laxative products. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 is a flow chart of the preparation method of the present invention; Figure 2 This is a statistical diagram of the results of Experiment 1 of the present invention; Figure 3 This is a statistical diagram of the results of Experiment 2 of the present invention; Figure 4 This is a statistical diagram of the results of Experiment 3 of the present invention; Figure 5 This is a statistical chart of the results of Experiment 4 of the present invention. DETAILED DESCRIPTION

[0058] The present invention will be further explained below in conjunction with specific embodiments. It will be appreciated by those skilled in the art that the following described embodiments are part of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. All reagents used are conventional products that can be purchased commercially.

[0059] Example 1: This example provides a laxative composition, comprising the following raw materials and their weight portions: 5 parts of composite fruit and vegetable fermentation liquid, 2 parts of composite fruit concentrated juice, 0.01 parts of composite probiotic powder, 17 parts of prebiotics, 0.1 parts of DL-malic acid, 0.05 parts of citric acid, 0.005 parts of sodium citrate, 0.01 parts of potassium sorbate, and 59 parts of water.

[0060] The composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of amomum villosum, 1.9 parts of sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase.

[0061] The composite fruit concentrated juice comprises the following raw materials and their weight portions: 50 parts of apple concentrated juice, 25 parts of strawberry concentrated juice, 12.5 parts of cranberry concentrated juice, and 12.5 parts of blueberry concentrated juice.

[0062] The composite probiotic powder comprises the following raw materials and their weight portions: 25 parts of Lactobacillus plantarum, 25 parts of Lactobacillus acidophilus, 25 parts of Bifidobacterium longum, and 25 parts of Lactobacillus rhamnosus.

[0063] The prebiotics include the following raw materials and their weight proportions: 8-12 parts of xylo-oligosaccharide, 3-5 parts of lactitol, 3-5 parts of L-arabinose, and 3-5 parts of sorbitol liquid.

[0064] And, if Figure 1 As shown, this embodiment also provides a preparation process of the laxative composition, comprising the following steps: S1. Prepare mixed solution A: Weigh enoki mushrooms, carrots, yam, cucumber, broccoli, mulberry, apple, pear, grapefruit, pineapple, papaya, orange, peach, lemon, grape, blueberry, and strawberry respectively, wash them, mix them together, add an equal amount of water to squeeze the juice, and then filter to obtain mixed solution A.

[0065] S2. Prepare mixed solution B: Weigh burdock root, cassia seed, liquorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and momordica grosvenori respectively, mix them together, soak them in water for 0.5 h, and then boil them at 80 ° C for 2 h until half of the water is left, stop boiling, and filter to obtain mixed solution B.

[0066] S3. Prepare mixed solution C: sterilize the mixed solution A and the mixed solution B under high pressure and high temperature at a sterilization temperature of 121° C. for 30 min to obtain a mixed solution C.

[0067] S4. Preparation of composite fruit and vegetable fermentation liquid: First, add 0.01 g of yeast to every 100 g of mixed liquid C for the first fermentation, and ferment at 20° C. for 4 days. During the yeast fermentation, add 0.1 g of lipase, 0.07 g of cellulase, and 0.05 g of pectinase for decomposition to strengthen the effective ingredients and enhance the nutritional essence.

[0068] Then, 0.015 g of lactic acid bacteria was added for the second fermentation, and the fermentation was carried out at a temperature of 25° C. for 72 hours, so as to reduce the nutrient components to small molecules. During the fermentation process, when the pH of the fermentation liquid reached 3, the fermentation was stopped. The fermented liquid was first filtered and then clarified to obtain a composite fruit and vegetable fermentation liquid.

[0069] S5. Mixing: Weigh the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder, prebiotics, DL-malic acid, citric acid, sodium citrate, potassium sorbate and water respectively, mix them evenly, and then sterilize and fill them to obtain the laxative composition product.

[0070] Example 2: This example provides a laxative composition, which is different from Example 1 in that it includes the following raw materials and their weight portions: 8 parts of composite fruit and vegetable fermentation liquid, 3 parts of composite fruit concentrated juice, 0.015 parts of composite probiotic powder, 23 parts of prebiotics, 0.125 parts of DL-malic acid, 0.08 parts of citric acid, 0.008 parts of sodium citrate, 0.015 parts of potassium sorbate, and 62 parts of water.

[0071] And, if Figure 1As shown, this embodiment also provides a preparation process of the laxative composition, comprising the following steps: S1. Prepare mixed solution A: Weigh enoki mushroom, carrot, yam, cucumber, broccoli, mulberry, apple, pear, grapefruit, pineapple, papaya, orange, peach, lemon, grape, blueberry, and strawberry respectively, wash them, mix them together, then add an equal amount of water to squeeze the juice, and filter to obtain mixed solution A.

[0072] S2. Prepare mixed solution B: Weigh burdock root, cassia seed, liquorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and momordica grosvenori respectively, mix them together, soak them in water for 0.8 h, then boil them at 85° C. for 1.5 h until half of the water is left, and then filter them to obtain mixed solution B.

[0073] S3. Prepare mixed solution C: sterilize the mixed solution A and the mixed solution B under high pressure and high temperature at a sterilization temperature of 121° C. for 30 min to obtain a mixed solution C.

[0074] S4. Preparation of composite fruit and vegetable fermentation liquid: First, add 0.01 g of yeast to every 100 g of mixed liquid C for the first fermentation, and ferment at 25° C. for 3 days. During the yeast fermentation, add 0.1 g of lipase, 0.07 g of cellulase, and 0.05 g of pectinase for decomposition to strengthen the effective ingredients and enhance the nutritional essence.

[0075] Then, 0.015 g of lactic acid bacteria was added for the second fermentation, and the fermentation was carried out at a temperature of 28°C for 60 hours to reduce the nutrients to small molecules. During the fermentation process, when the pH of the fermentation liquid reached 3.2, the fermentation was stopped. The fermented liquid was first filtered and then clarified to obtain a composite fruit and vegetable fermentation liquid.

[0076] S5. Mixing: Weigh the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder, prebiotics, DL-malic acid, citric acid, sodium citrate, potassium sorbate and water respectively, mix them evenly, and then sterilize and fill them to obtain the laxative composition product.

[0077] Example 3: This example provides a laxative composition, which is different from Example 1 in that it includes the following raw materials and their weight portions: 10 parts of composite fruit and vegetable fermentation liquid, 4 parts of composite fruit concentrated juice, 0.02 parts of composite probiotic powder, 27 parts of prebiotics, 0.15 parts of DL-malic acid, 0.1 parts of citric acid, 0.01 parts of sodium citrate, 0.02 parts of potassium sorbate, and 65 parts of water.

[0078] And, if Figure 1 As shown, this embodiment also provides a preparation process of the laxative composition, comprising the following steps: S1. Prepare mixed solution A: Weigh enoki mushroom, carrot, yam, cucumber, broccoli, mulberry, apple, pear, grapefruit, pineapple, papaya, orange, peach, lemon, grape, blueberry, and strawberry respectively, wash them, mix them together, then add an equal amount of water to squeeze the juice, and filter to obtain mixed solution A.

[0079] S2. Prepare mixed solution B: Weigh burdock root, cassia seed, licorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and momordica grosvenori respectively, mix them together, soak them in water for 1 h, and then boil them at 90° C. for 1 h until the amount of water is reduced to half, and filter them to obtain mixed solution B.

[0080] S3. Prepare mixed solution C: sterilize the mixed solution A and the mixed solution B under high pressure and high temperature at a sterilization temperature of 121° C. for 30 min to obtain a mixed solution C.

[0081] S4. Preparation of composite fruit and vegetable fermentation liquid: First, add 0.01 g of yeast to every 100 g of mixed liquid C for the first fermentation, and ferment at 30° C. for 2 days. During the yeast fermentation, add 0.1 g of lipase, 0.07 g of cellulase, and 0.05 g of pectinase for decomposition to strengthen the effective ingredients and enhance the nutritional essence.

[0082] Then, 0.015 g of lactic acid bacteria was added for the second fermentation, and the fermentation was carried out at a temperature of 30°C for 72 hours to reduce the nutrients to small molecules. During the fermentation process, when the pH of the fermentation liquid reached 3.4, the fermentation was stopped. The fermented liquid was first filtered and then clarified to obtain a composite fruit and vegetable fermentation liquid.

[0083] S5. Mixing: Weigh the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder, prebiotics, DL-malic acid, citric acid, sodium citrate, potassium sorbate and water respectively, mix them evenly, and then sterilize and fill them to obtain the laxative composition product.

[0084] Comparative Example 1: This comparative example provides a laxative composition, which is different from Example 1 in that it includes the following raw materials and their weight portions: 5 parts of composite fruit and vegetable fermentation liquid, 0.01 parts of composite probiotic powder, 17 parts of prebiotics, 0.1 parts of DL-malic acid, 0.05 parts of citric acid, 0.005 parts of sodium citrate, 0.01 parts of potassium sorbate, and 59 parts of water, that is, no composite fruit concentrated juice is added.

[0085] Comparative Example 2: This comparative example provides a laxative composition, which is different from Example 1 in that it includes the following raw materials and their weight portions: 5 parts of composite fruit and vegetable fermentation liquid, 2 parts of composite fruit concentrated juice, 17 parts of prebiotics, 0.1 parts of DL-malic acid, 0.05 parts of citric acid, 0.005 parts of sodium citrate, 0.01 parts of potassium sorbate, and 59 parts of water, that is, no composite probiotic powder is added.

[0086] Comparative Example 3: This comparative example provides a laxative composition, which is different from Example 1 in that it includes the following raw materials and their weight portions: 5 parts of composite fruit and vegetable fermentation liquid, 2 parts of composite fruit concentrated juice, 0.01 parts of composite probiotic powder, 0.1 parts of DL-malic acid, 0.05 parts of citric acid, 0.005 parts of sodium citrate, 0.01 parts of potassium sorbate, and 59 parts of water, that is, no prebiotics are added.

[0087] Comparative Example 4: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, burdock root is missing.

[0088] Comparative Example 5: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of Houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of Perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of Momordica grosvenori, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, Cassia seed is missing.

[0089] Comparative Example 6: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, licorice is missing.

[0090] Comparative Example 7: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, lack of almonds.

[0091] Comparative Example 8: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, lack of hemp seed.

[0092] Comparative Example 9: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 3 parts of houttuynia, 1 part of amomum, 1.9 parts of sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, honeysuckle is missing.

[0093] Comparative Example 10: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of Perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of Momordica grosvenori, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, Houttuynia cordata is missing.

[0094] Comparative Example 11: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, it lacks Amomum villosum.

[0095] Comparative Example 12: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, there is no Sterculia lychnophora.

[0096] Comparative Example 13: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, there is no perilla seed.

[0097] Comparative Example 14: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, dandelion is missing.

[0098] Comparative Example 15: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, there is no purslane.

[0099] Comparative Example 16: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, there is no jujube.

[0100] Comparative Example 17: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of Houttuynia cordata, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of Perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of Momordica grosvenori, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, hawthorn is missing.

[0101] Comparative Example 18: This comparative example provides a laxative composition, which is different from Example 1 in that the composite fruit and vegetable fermentation liquid includes the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia, 1 part of Amomum villosum, 1.9 parts of Sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase, that is, there is no monk fruit.

[0102] Experiment 1: Mouse experiment (improving constipation).

[0103] Experimental method: Kunming mice weighing 18-22g, half male and half female, were randomly divided into blank control group, model group, low-, medium- and high-dose groups. They were adaptively fed for 3 days and fasted but not watered for 12 hours before the experiment. Except for the blank control group, the mice in other groups were gavaged with compound diphenoxylate (10 mg / kg body weight) once to establish the model, and the gavage was continued for 3 days. The mice in the blank control group were given an equal volume of distilled water.

[0104] After 3 days of experimental modeling, the mice in the blank control group and the model group were given (20 mL / kg body weight) of distilled water, and the mice in the low-, medium-, and high-dose groups were given (1, 2, and 4 g / kg body weight) of the laxative composition prepared in Example 1, respectively, for 3 consecutive days.

[0105] 30 minutes after the last administration, each group of mice was given (0.2 mL / 10 g body weight) of carbon powder suspension, and all mice were placed in a metabolic cage with filter paper on the bottom for observation. They were allowed to eat and drink freely, and the time of the first defecation of each mouse, the number of defecations of each mouse within 6 hours, and the total weight of the defecation of the mice within 6 hours were observed and recorded.

[0106] The experimental results are shown in the following table.

[0107]

[0108] like Figure 2 As shown, compared with the blank control group mice, the model control group mice had a prolonged first defecation time and a reduced amount of stool within 6 hours. Compared with the model control group mice, the low, medium and high dose groups had a shortened first defecation time and an increased stool weight within 6 hours, indicating that the laxative composition prepared in Example 1 has the effect of clearing intestinal waste and laxative.

[0109] Experiment 2: Mouse experiment (regulating intestinal flora).

[0110] Experimental method: 40 male Kunming mice weighing 18-22g were selected and randomly divided into four groups, including a normal control group, a high-dose group, a medium-dose group, and a low-dose group. The mice in the high-dose, medium-dose, and low-dose groups were given (4, 2, and 1 g / kg body weight) of the laxative composition prepared in Example 1, respectively.

[0111] After the animals in each group were divided into groups and before the test substances were administered, 0.1 g of feces from each mouse was aseptically collected and inoculated on the culture medium at appropriate dilutions according to the method for checking intestinal flora (Bifidobacterium, Lactobacillus, Enterobacter, Enterococcus) in the "Regulating the Function of Intestinal Flora" of the "Technical Specifications for Inspection and Evaluation of Health Foods" (2003 edition). After cultivation, the colonies were identified and counted by colony morphology, Gram staining microscopy, biochemical reaction, etc., and the number of bacteria per gram of wet feces was calculated as the colony situation before the test samples were administered.

[0112] The test samples were continuously gavaged for 14 days, and 24 hours after the last administration, 0.1 g of feces from each mouse was aseptically collected again, and the intestinal flora was detected using the same method. The changes in bifidobacteria, lactobacilli, enterococci, and enterobacteria in feces were compared, and the unit was (log 10 cfu / g) The experimental results are shown in the following table.

[0113]

[0114] like Figure 3 As shown, the laxative composition prepared in Example 1 can significantly increase the number of bifidobacteria, lactobacilli, enterococci and enterobacteria in the feces of mice after 14 days compared with the control group. At the same time, the number of bifidobacteria and lactobacilli also increases significantly compared with before the experiment, indicating that the laxative composition of the present invention has the effect of regulating intestinal flora.

[0115] Experiment 3: Small intestinal propulsion experiment.

[0116] Experimental method: Kunming mice weighing 18-22g, half male and half female, were randomly divided into blank control group, model group, and experimental groups 1-19. They were adaptively fed for 3 days and fasted but not watered for 12 hours before the experiment. Except for the blank control group, the mice in other groups were gavaged with compound diphenoxylate (10 mg / kg body weight) once to establish the model, and the gavage was continued for 3 days. The mice in the blank control group were given an equal volume of distilled water.

[0117] After 3 days of experimental modeling, the mice in the blank control group and the model group were given (20 mL / kg body weight) of distilled water, and the mice in the experimental groups 1-19 were given (2 g / kg body weight) of the laxative composition prepared in Example 1 and Comparative Examples 1-18, respectively, for 3 consecutive days.

[0118] 30 minutes after the last administration, mice in each group were given ink suspension at a rate of (20 mL / kg body weight). After an interval of 30 minutes, the mice were killed by cervical dislocation, the abdominal cavity was immediately opened to separate the mesentery, the intestinal tube from the cardia to the ileocecal part was cut and gently pulled into a straight line, the length of the intestinal tube was measured as the total length of the small intestine, and the length from the pylorus to the carbon front was the ink advancement length, and the ink advancement rate was calculated, ink advancement rate / %=ink advancement length / total length of small intestine × 100%.

[0119] The experimental results are shown in the following table.

[0120]

[0121] like Figure 4 As shown, compared with the blank control group mice, the ink advancement rate of the model control group mice was low, and compared with the model control group mice, the ink advancement rate of the experimental group 1-19 mice was high, but compared with the experimental group 1, the ink advancement rate of the experimental group 2-4 mice was low, indicating that: in the intestinal laxative composition of the present invention, if any one of the composite fruit and vegetable fermented liquid, composite probiotic powder, and prebiotics is missing, the product effect will be greatly reduced, and when the three ingredients coexist, a synergistic promotion effect can be produced. In addition, compared with the experimental group 1, the ink advancement rate of the experimental groups 5-19 is low, because there are 15 main active ingredients in the composite fruit and vegetable fermented liquid, and the lack of any one of them will weaken the effect of the composite fruit and vegetable fermented liquid.

[0122] Experiment 4: Human food tasting experiment.

[0123] Experimental subjects: 150 voluntary subjects were selected, with a similar male-female ratio, mostly aged between 18 and 50 years old, all with varying degrees of constipation, dry stool, and stool once every few days. The subjects were randomly divided into 3 groups, namely, test group A, test group B, and test group C.

[0124] Experimental method: Experimental groups AC took the laxative composition prepared in Examples 1-3, twice a day, 1 bag each time, 25 g per bag, for 2 weeks.

[0125] Efficacy criteria: Cured: smooth bowel movements, once a day, moderate hardness and disappearance of constipation complications. Effective: smooth bowel movements, once a day or once every two days, with occasional dryness. Ineffective: obvious constipation symptoms, no improvement.

[0126] The experimental results are shown in the following table.

[0127]

[0128] like Figure 5As shown, the total effective rate of promoting excretion of patients by the laxative composition prepared in Examples 1-3 is as high as 94%, indicating that the laxative composition of the present invention has the effect of clearing intestinal waste and moistening the intestines to promote bowel movements.

[0129] Finally, it should be noted that the above embodiments are only the best specific implementation methods of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A laxative composition, characterized in that: The invention comprises the following raw materials and their weight parts: 5-10 parts of compound fruit and vegetable fermentation liquid, 2-4 parts of compound fruit concentrated juice, 0.01-0.02 parts of compound probiotic powder, 17-27 parts of prebiotics, 0.1-0.15 parts of DL-malic acid, 0.05-0.1 parts of citric acid, 0.005-0.01 parts of sodium citrate, 0.01-0.02 parts of potassium sorbate and 59-65 parts of water; The composite fruit and vegetable fermentation liquid comprises the following raw materials and their weight portions: 3 parts of Flammulina velutipes, 1 part of carrot, 3 parts of yam, 2.5 parts of cucumber, 1.2 parts of broccoli, 0.8 parts of mulberry, 2 parts of apple, 2 parts of pear, 2 parts of grapefruit, 2 parts of pineapple, 2 parts of papaya, 2 parts of orange, 2 parts of peach, 2 parts of lemon, 2 parts of grape, 2 parts of blueberry, 2 parts of strawberry, 30 parts of burdock root, 5 parts of cassia seed, 3 parts of licorice, 3 parts of almond, 1.5 parts of hemp seed, 2.5 parts of honeysuckle, 3 parts of houttuynia cordata, 1 part of amomum villosum, 1.9 parts of sterculia lychnophora, 0.5 parts of perilla seed, 1.5 parts of dandelion, 2 parts of purslane, 2 parts of jujube, 2 parts of hawthorn, 2 parts of monk fruit, 0.1 parts of lipase, 0.07 parts of cellulase, and 0.05 parts of pectinase; The composite fruit concentrated juice comprises the following raw materials and their weight parts: 50 parts of apple concentrated juice, 25 parts of strawberry concentrated juice, 12.5 parts of cranberry concentrated juice, and 12.5 parts of blueberry concentrated juice; The composite probiotic powder comprises the following raw materials and their weight parts: 25 parts of Lactobacillus plantarum, 25 parts of Lactobacillus acidophilus, 25 parts of Bifidobacterium longum, and 25 parts of Lactobacillus rhamnosus; The prebiotics include the following raw materials and their weight proportions: 8-12 parts of xylo-oligosaccharide, 3-5 parts of lactitol, 3-5 parts of L-arabinose, and 3-5 parts of sorbitol liquid.

2. A method for preparing a laxative composition according to claim 1, characterized in that: The steps include: S1. Prepare mixed solution A: weigh enoki mushroom, carrot, yam, cucumber, broccoli, mulberry, apple, pear, grapefruit, pineapple, papaya, orange, peach, lemon, grape, blueberry, and strawberry respectively, wash them, mix them evenly, add water of the same mass to squeeze the juice, and then filter them to obtain mixed solution A; S2, preparing mixed solution B: respectively weighing burdock root, cassia seed, liquorice, almond, hemp seed, honeysuckle, houttuynia, amomum, sterculia lychnophora, perilla seed, dandelion, purslane, jujube, hawthorn, and momordica grosvenori, and mixing them evenly, then adding water to soak, boiling, and filtering to obtain mixed solution B; S3, preparing mixed solution C: mixing mixed solution A and mixed solution B evenly, and then sterilizing them under high pressure and high temperature to obtain mixed solution C; S4, preparing a composite fruit and vegetable fermentation liquid: first adding yeast to the mixed liquid C for a first fermentation, then adding lactic acid bacteria for a second fermentation, and then filtering and clarifying the fermentation liquid to obtain a composite fruit and vegetable fermentation liquid; S5. Mixing: Weigh the composite fruit and vegetable fermentation liquid, composite fruit concentrated juice, composite probiotic powder, prebiotics, DL-malic acid, citric acid, sodium citrate, potassium sorbate and water respectively, mix them evenly, sterilize them, and fill them to obtain a laxative composition product.

3. The method for preparing a laxative composition according to claim 2, characterized in that: In step S2, the soaking time is 0.5-1h.

4. The method for preparing a laxative composition according to claim 2 or 3, characterized in that: In step S2, the boiling temperature is 80-90°C and the boiling time is 1-2h, and the boiling is stopped when half of the added water remains.

5. The method for preparing a laxative composition according to claim 2, characterized in that: In step S3, the sterilization temperature is 121° C. and the sterilization time is 30 min.

6. The method for preparing a laxative composition according to claim 2, characterized in that: In step S4, when the yeast is fermenting for the first time, 0.01 g of yeast is added to every 100 g of mixed solution C, the fermentation temperature is 20-30° C., and the fermentation time is 2-4 days.

7. The method for preparing a laxative composition according to claim 2 or 6, characterized in that: In step S4, when the yeast is undergoing the first fermentation, 0.1 g of lipase, 0.07 g of cellulase, and 0.05 g of pectinase are added to every 100 g of the mixed solution C.

8. The method for preparing a laxative composition according to claim 2, characterized in that: In step S4, when the lactic acid bacteria undergo the second fermentation, 0.015 g of lactic acid bacteria is added to every 100 g of the mixed solution C, the fermentation temperature is 25-30° C., and the fermentation time is 48-72 h.

9. The method for preparing a laxative composition according to claim 2 or 8, characterized in that: In step S4, when the lactic acid bacteria undergo a second fermentation, when the pH of the fermentation liquid reaches 3-3.4, the fermentation is stopped.

10. Use of the laxative composition according to claim 1 in laxative products.

Images