Radix astragali and cassia twig five-material decoction added with probiotics and processing method of radix astragali and cassia twig five-material decoction

Abstract

The invention discloses radix astragali and cassia twig five-material soup added with probiotics and a processing method of the radix astragali and cassia twig five-material soup. The formula comprises radix astragali, cassia twigs, Chinese herbaceous peony, fresh ginger, Chinese dates, radix angelicae sinensis, rhizoma chuanxiong and flos carthami. The method comprises the following steps: step 1, weighing raw materials; step 2, treating and soaking; step 3, performing decoction treatment; step 4, microwave extraction; step 5, mixing liquid medicine; step 6, concentrating and seasoning; step 7, preparing a microbial inoculum; step 8, adding beneficial bacteria; and step 9, filling and storing. On the basis of a traditional formula, the angelica sinensis, the ligusticum wallichii and the safflower are added to serve as auxiliary medicines, and the medicinal effect of the five-material decoction is enriched; a microwave extraction mode is used for replacing twice decoction, so that the waste of medicine effect is avoided; by adding a vacuum concentration process, the radix astragali and cassia twig five-material decoction is prepared into the oral liquid, a blending fungicide is added, and probiotics are added into the five-material decoction, so that the beneficial bacteria can be planted in the intestinal tract and reproductive system of a human body after the oral liquid is taken, an exact health effect can be generated, and the micro-ecological balance of a host can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of pharmaceutical processing, in particular to an astragalus, cinnamon twig and five-ingredient decoction with probiotics added therein and a processing method thereof. Background Art

[0002] Huangqi Guizhi Wuwu Decoction is one of the classic prescriptions of traditional Chinese medicine. It is mainly used to treat blood stasis syndrome, which is manifested by numbness of the skin, slight aversion to wind and cold, pale tongue with white fur, weak and tight pulse, etc. In addition, this prescription is also suitable for limb joint pain, numbness, weakness and other symptoms caused by insufficient qi and blood, wind and cold stagnation; modern clinical practice often uses it to treat dermatitis, peripheral neuritis, sequelae of stroke, diabetic peripheral neuropathy, cervical spondylosis, lumbar disc herniation, Raynaud's syndrome, facial nerve paralysis and other diseases, and the syndrome is diagnosed as qi deficiency and blood stasis, wind and cold stagnation; the existing Huangqi Guizhi Wuwu Decoction and its processing method can basically meet the daily medical use, but there are still certain shortcomings. First, the existing Huangqi Guizhi Wuwu Decoction The ingredients of Wuwu Decoction are relatively simple. Due to the limitation of its own use, the edible effect of the prepared medicine is simple and it does not have the effect of promoting blood circulation and removing blood stasis. Secondly, as a traditional Chinese medicine, Huangqi Guizhi Wuwu Decoction relies on two decoctions to extract the effective ingredients in the medicine during the preparation process. However, the decoction operation is simple and it is difficult to fully extract the effective ingredients of the medicine, resulting in a waste of medicinal effects. Thirdly, the preparation process of Huangqi Guizhi Wuwu Decoction is simple, and in order to reduce damage to the stomach and intestines, it needs to be taken hot, which affects the user experience, and the beneficial bacteria added with stomach-nourishing function will be inactivated during the hot serving process. Therefore, it is necessary to design an Astragalus Guizhi Wuwu Decoction with added probiotics and a processing method thereof. Summary of the invention

[0003] The object of the present invention is to provide a Huangqi Guizhi Wuwu Decoction with added probiotics and a processing method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a Huangqi Guizhi Wuwu Decoction with added probiotics, the formula comprising: Huangqi, Guizhi, Paeony, Ginger, Jujube, Angelica, Chuanxiong and Safflower; the weight proportions of each component are: 8-10 parts of Huangqi, 8-10 parts of Guizhi, 8-10 parts of Paeony, 16-20 parts of Ginger, 16-20 parts of Jujube, 2-3 parts of Angelica, 5-7 parts of Chuanxiong and 3-5 parts of Safflower.

[0005] A processing method of Huangqi Guizhi Wuwu Decoction with added probiotics comprises the following steps: step 1, weighing raw materials; step 2, treating and soaking; step 3, decocting; step 4, microwave extraction; step 5, mixing medicinal liquids; step 6, concentrating and seasoning; step 7, preparing bacterial agents; step 8, adding beneficial bacteria; step 9, filling and storing;

[0006] In the above step 1, 8-10 parts of astragalus, 8-10 parts of cassia twig, 8-10 parts of peony, 16-20 parts of ginger, 16-20 parts of jujube, 2-3 parts of angelica, 5-7 parts of chuanxiong and 3-5 parts of safflower are weighed according to the weight of each group and set aside;

[0007] In the above step 2, the astragalus, cinnamon twig, peony root, ginger, jujube, angelica, ligusticum chuanxiong and safflower prepared in the above step 1 are pre-treated respectively, and after the pre-treatment, they are placed in a soaking basin and soaked in clean water;

[0008] In the above step 3, all the contents in the soaking basin in the above step 2 are poured into the ceramic pot, and then the liquid in the ceramic pot is boiled over high heat, and then the high heat is maintained to continue boiling, and then the heat is reduced to continue decocting, and after continuous decocting, the decocted medicinal residue and the decoction are filtered to obtain the decocted medicinal residue and the decoction liquid, which are set aside;

[0009] In the above step 4, the decocted medicinal residue prepared in the above step 3 is subjected to extrusion treatment, and the separated liquid obtained after the extrusion treatment is used for standby, and the decocted medicinal residue after the extrusion treatment is placed in a drying oven for drying treatment, and then crushed by a pulverizer, and then water is selected as a solvent and added to the reaction bottle, and then the separated liquid is added to the reaction bottle, and then a microwave extraction device is used for extraction treatment, and after the extraction treatment, the extraction liquid is separated to obtain a standby extract;

[0010] In the above step 5, the extract obtained in the above step 4 and the decoction obtained in the step 3 are put into a stirring device together, and then continuously stirred in the stirring device to obtain a mixed medicinal solution for standby use;

[0011] In the above step 6, the mixed liquid medicine obtained in the above step 5 is placed in a concentration tank for reduced pressure concentration, and honey is added for seasoning after reduced pressure concentration, and the Wuwu Decoction oral liquid is obtained after seasoning for standby use;

[0012] In the above step 7, lactic acid bacteria and bifidobacteria are selected as the prepared beneficial bacteria, and the two beneficial bacteria are cultured respectively, and the obtained lactic acid bacteria agent and bifidobacterium agent are mixed in a ratio of 1:1 after the culture, and the prepared bacterial agent is obtained after the mixing, and is set aside;

[0013] In the above step eight, the prepared bacterial agent obtained in the above step eight is added to the Wuwu Decoction oral liquid prepared in step six, and then mixed and stirred to obtain a mixed stock solution for standby use;

[0014] In the above step nine, the mixed stock solution obtained in the above step eight is placed in a glass bottle, which is then sealed to obtain the Huangqi Guizhi Wuwu Decoction with added probiotics, which is then stored in a cool place.

[0015] As a further technical solution of the present invention, in the step 2, the pretreatment of astragalus requires washing the dust remaining on the surface with clean water, and then slicing; the pretreatment of cinnamon twig requires washing the dust remaining on the surface with clean water, and then using a grinder to crush it; the pretreatment of peony requires washing the dust remaining on the surface with clean water; the pretreatment of ginger requires peeling the ginger and slicing it after peeling; the pretreatment of jujube requires washing the dust remaining on the surface with clean water, and then removing the core; the pretreatment of angelica and Chuanxiong requires washing the dust remaining on the surface with clean water; the pretreatment of safflower requires gently washing with water using a sieve; the soaking treatment requires putting the pretreated astragalus, cinnamon twig, peony, ginger, jujube, angelica, Chuanxiong and safflower into a soaking basin together, and the amount of clean water added is 5 to 10 cm above the top of the medicinal materials, and the soaking treatment time is 30 to 60 minutes.

[0016] As a further technical solution of the present invention, in step three, the duration of continuous boiling is 3 to 5 minutes, and the duration of continuous decocting after turning to low heat is 45 to 60 minutes.

[0017] As a further technical solution of the present invention, in step 4, the temperature in the drying oven during the drying process is 65-70°C, and the drying time is 40-50 minutes. The amount of clean water used as a solvent is 400%-450% of the mass fraction of the solid matter. The microwave power of the microwave extraction device during the extraction process is 500-1000W. After pre-extraction for 1-2 minutes, the power is increased to 1200-1300W and continued for 10-15 minutes to complete the entire extraction process.

[0018] As a further technical solution of the present invention, in the step five, the rotation speed of continuous stirring is 30 to 60 r / min, and the continuous stirring time is 18 to 25 min.

[0019] As a further technical solution of the present invention, in step six, the evaporation rate of the concentration tank during the reduced pressure concentration process is 70 to 350 kg / h, the steam pressure of the concentration tank is 0.15 MPa, and the vacuum degree in the concentration tank is -0.5 to -0.08 mmHg; when honey is added for seasoning, the amount of honey added is 2% to 5% of the volume of the concentrated liquid.

[0020] As a further technical solution of the present invention, in the step seven, during the culture of lactic acid bacteria, firstly, an agar medium is selected for sterilization treatment, and after the sterilization treatment, the pH value of the agar medium is adjusted to 6.5-7.0, and after the lactic acid bacteria are inoculated, fermentation culture is carried out in a temperature range of 35-37°C. During the fermentation culture process, the pH value of the culture medium is always maintained at 6.5-7.0, and during the fermentation culture process, the culture medium needs to be stirred at a low speed at a stirring rate of 5-8r / min, and the lactic acid bacteria agent is obtained by centrifugal filtration after fermentation culture.

[0021] As a further technical solution of the present invention, in the step seven, during the culturing of bifidobacteria, firstly, MRS medium is selected as the culture medium of bifidobacteria, then the MRS medium is dissolved in a phosphate buffer solution with a pH value of 6.0 to 7.0, and then the culture liquid is placed in a flask for sterilization. After inoculating bifidobacteria, constant temperature culture is carried out in a water bath at 37 to 41°C for 1200 to 1800 minutes, and after constant temperature culture, the bifidobacterium agent is obtained by centrifugal filtration.

[0022] As a further technical solution of the present invention, in step eight, the amount of the prepared bacterial agent added to the Wuwu Decoction oral liquid is 0.5% to 0.8% by volume of the Wuwu Decoction oral liquid; the rotation speed during the mixing and stirring process is 30 to 60 r / min, and the mixing and stirring time is 18 to 25 min.

[0023] Compared with the prior art, the invention has the following beneficial effects: the Huangqi Guizhi Wuwu Decoction with added probiotics and the processing method thereof, on the basis of the traditional formula, adds angelica, chuanxiong and safflower as auxiliary drugs, which can increase the content of trace elements such as vitamin B12, iron and zinc in the medicine after being mixed and decocted with the raw materials of the Wuwu Decoction, help neutralize free radicals, reduce oxidative stress, and have anti-inflammatory properties, so that the medicine has the effect of promoting blood circulation and removing blood stasis, and enriches the medicinal efficacy of the traditional Wuwu Decoction; by using microwave extraction to replace the two decoctions in the traditional preparation process, the rupture of the cell wall of the medicinal material is promoted, the dissolution of the effective ingredients is accelerated, the extraction efficiency is improved, and the waste of the efficacy is avoided; by adding a reduced pressure concentration process after preparation, the Huangqi Guizhi Wuwu Decoction is prepared into an oral liquid, and then the prepared bifidobacterium agent and lactic acid bacteria agent are mixed and added to the oral liquid, probiotics are added to the Wuwu Decoction, and after taking it, the beneficial bacteria can be colonized in the intestinal tract and reproductive system of the human body, which can produce definite health effects and improve the host microecological balance. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a flow chart of the processing method of the present invention. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] See also Figure 1 , the present invention provides a technical solution:

[0027] Embodiment 1:

[0028] A Huangqi Guizhi Wuwu Decoction with added probiotics comprises: Huangqi, Guizhi, Paeonia lactiflora, ginger, jujube, Chinese angelica, Chuanxiong and safflower; the weight proportions of the components are: 10 parts of Huangqi, 10 parts of Guizhi, 10 parts of Paeonia lactiflora, 20 parts of ginger, 20 parts of jujube, 3 parts of Chinese angelica, 7 parts of Chuanxiong and 5 parts of safflower.

[0029] A processing method of Huangqi Guizhi Wuwu Decoction with added probiotics comprises the following steps: step 1, weighing raw materials; step 2, treating and soaking; step 3, decocting; step 4, microwave extraction; step 5, mixing medicinal liquids; step 6, concentrating and seasoning; step 7, preparing bacterial agents; step 8, adding beneficial bacteria; step 9, filling and storing;

[0030] In the above step 1, 10 parts of astragalus, 10 parts of cassia twig, 10 parts of peony, 20 parts of ginger, 20 parts of jujube, 3 parts of angelica, 7 parts of ligusticum chuanxiong and 5 parts of safflower are weighed according to the weight of each group and set aside;

[0031] In the above step 2, the astragalus, cinnamon twig, peony, ginger, jujube, angelica, chuanxiong and safflower prepared in the above step 1 are pretreated respectively, and after the pretreatment, they are placed in a soaking basin and soaked with clean water; the pretreatment of astragalus requires washing the dust remaining on the surface with clean water, and then slicing; the pretreatment of cinnamon twig requires washing the dust remaining on the surface with clean water, and then using a crusher to crush it; the pretreatment of peony requires washing the dust remaining on the surface with clean water; the pretreatment of ginger requires The ginger was peeled and then sliced; the pretreatment of jujube required the use of clean water to wash away the dust remaining on the surface, and then the core was removed; the pretreatment of angelica and Chuanxiong required the use of clean water to wash away the dust remaining on the surface; the pretreatment of safflower required the use of a sieve to gently rinse with water; the soaking treatment required the pretreated astragalus, cassia twig, peony, ginger, jujube, angelica, Chuanxiong and safflower to be placed in a soaking basin together, and the amount of clean water added was 10 cm above the top of the medicinal materials, and the soaking treatment time was 60 minutes;

[0032] In the above step 3, all the contents in the soaking basin in the above step 2 are poured into the ceramic pot, and then the liquid in the ceramic pot is boiled over high heat, and then the high heat is maintained for continuous boiling, and then the heat is reduced for continuous decoction, and after continuous decoction, the decoction residue and the decoction liquid are obtained by filtering, and the decoction residue and the decoction liquid are respectively set aside; the duration of continuous boiling is 5 minutes, and the duration of continuous decoction after reducing the heat is 60 minutes;

[0033] In the above step 4, the decocted medicinal residue prepared in the above step 3 is extruded, and the separated liquid obtained after the extrusion treatment is used for standby, and the decocted medicinal residue after the extrusion treatment is placed in a drying oven for drying treatment, and then crushed by a pulverizer, and after crushing, clean water is selected as a solvent and added to the reaction bottle, and then the separated liquid is added to the reaction bottle, and then extracted by a microwave extraction device, and separated after the extraction treatment to obtain an extract for standby; the temperature in the drying oven during the drying process is 70°C, and the drying time is 50 minutes, the amount of clean water used in the process of using clean water as a solvent is 450% of the mass fraction of the solid matter, and the microwave power of the microwave extraction device during the extraction process is 1000W, and after 2 minutes of pre-extraction, the power is increased to 1300W and continued for 15 minutes to complete the entire extraction process;

[0034] In the above step 5, the extract obtained in the above step 4 and the decoction obtained in the step 3 are put into a stirring device together, and then continuously stirred in the stirring device to obtain a mixed medicinal solution for standby use; the speed of continuous stirring is 60r / min, and the continuous stirring time is 25min;

[0035] wherein in the above step 6, the mixed liquid medicine obtained in the above step 5 is placed in a concentration tank for vacuum concentration, honey is added for seasoning after vacuum concentration, and Wuwutang oral liquid is obtained after seasoning for standby use; the evaporation rate of the concentration tank during the vacuum concentration process is 350kg / h, the steam pressure of the concentration tank is 0.15Mpa, and the vacuum degree in the concentration tank is -0.08mmHg; when honey is added for seasoning, the amount of honey added is 5% of the volume of the concentrate;

[0036] Wherein in the above step seven, lactic acid bacteria and bifidobacterium are selected as the deployment beneficial bacteria, and the two beneficial bacteria are cultured respectively. After the culture, the obtained lactic acid bacteria agent is mixed with the bifidobacterium agent in a 1:1 ratio, and the deployment agent is obtained after mixing for standby; in the process of culturing lactic acid bacteria, firstly, agar medium is selected for sterilization, and the pH value of the agar medium is adjusted to 7.0 after the sterilization, and fermentation culture is carried out in a temperature range of 37° C. after inoculating lactic acid bacteria, and the pH value in the medium is always kept at 7.0 during the fermentation culture process, and the medium needs to be stirred at a low speed at a stirring rate of 8r / min during the fermentation culture process, and the lactic acid bacteria agent is obtained by centrifugal filtration after fermentation culture; in the process of culturing bifidobacteria, MRS medium is first selected as the culture medium liquid of bifidobacteria, and then the MRS medium is dissolved in a phosphate buffer solution with a pH value of 7.0, and then the culture liquid is placed in a flask for sterilization, and after inoculating bifidobacteria, constant temperature culture is carried out in a water bath at 41° C. for 1800 min, and the bifidobacterium agent is obtained by centrifugal filtration after constant temperature culture;

[0037] In the above step eight, the prepared bacterial agent obtained in the above step eight is added to the Wuwu Decoction oral liquid prepared in step six, and then mixed and stirred to obtain a mixed stock solution for standby use; when the prepared bacterial agent is added to the Wuwu Decoction oral liquid, the amount of the prepared bacterial agent added is 0.8% of the volume of the Wuwu Decoction oral liquid; the speed during the mixing and stirring process is 60r / min, and the mixing and stirring time is 25min;

[0038] In the above step nine, the mixed stock solution obtained in the above step eight is placed in a glass bottle, which is then sealed to obtain the Huangqi Guizhi Wuwu Decoction with added probiotics, which is then stored in a cool place.

[0039] Embodiment 2:

[0040] A probiotic-added Huangqi Guizhi Wuwu Decoction comprises: Huangqi, Guizhi, Paeonia lactiflora, ginger, jujube, Chinese angelica, Chuanxiong and safflower; the weight proportions of the components are: 8 parts of Huangqi, 8 parts of Guizhi, 8 parts of Chinese angelica, 16 parts of ginger, 16 parts of jujube, 2 parts of Chinese angelica, 5 parts of Chuanxiong and 3 parts of safflower.

[0041] A processing method of Huangqi Guizhi Wuwu Decoction with added probiotics comprises the following steps: step 1, weighing raw materials; step 2, treating and soaking; step 3, decocting; step 4, microwave extraction; step 5, mixing medicinal liquids; step 6, concentrating and seasoning; step 7, preparing bacterial agents; step 8, adding beneficial bacteria; step 9, filling and storing;

[0042] In the above step 1, 8 parts of astragalus, 8 parts of cassia twig, 8 parts of peony, 16 parts of ginger, 16 parts of jujube, 2 parts of angelica, 5 parts of ligusticum chuanxiong and 3 parts of safflower are weighed according to the weight of each group and set aside;

[0043] In the above step 2, the astragalus, cinnamon twig, peony, ginger, jujube, angelica, chuanxiong and safflower prepared in the above step 1 are pretreated respectively, and after the pretreatment, they are placed in a soaking basin and soaked with clean water; the pretreatment of astragalus requires washing the dust remaining on the surface with clean water, and then slicing; the pretreatment of cinnamon requires washing the dust remaining on the surface with clean water, and then using a crusher to crush; the pretreatment of peony requires washing the dust remaining on the surface with clean water; the pretreatment of ginger requires The ginger was peeled and sliced ​​after peeling; the pretreatment of jujube required the use of clean water to wash away the dust remaining on the surface, and then the core was removed; the pretreatment of angelica and Chuanxiong required the use of clean water to wash away the dust remaining on the surface; the pretreatment of safflower required the use of a sieve to gently rinse with water; the soaking treatment required the pretreated astragalus, cassia twig, peony, ginger, jujube, angelica, Chuanxiong and safflower to be placed in a soaking basin together, and the amount of clean water added was 5 cm above the top of the medicinal materials, and the soaking treatment time was 30 minutes;

[0044] In the above step 3, all the contents in the soaking basin in the above step 2 are poured into the ceramic pot, and then the liquid in the ceramic pot is boiled over high heat, and then the high heat is maintained for continuous boiling, and then the heat is reduced for continuous decoction, and after continuous decoction, the decoction residue and the decoction liquid are obtained by filtering, and the decoction residue and the decoction liquid are respectively set aside; the duration of continuous boiling is 3 minutes, and the duration of continuous decoction after reducing the heat is 45 minutes;

[0045] In the above step 4, the decocted medicinal residue prepared in the above step 3 is extruded, and the separated liquid obtained after the extrusion treatment is used for standby, and the decocted medicinal residue after the extrusion treatment is placed in a drying oven for drying treatment, and then crushed by a pulverizer, and after crushing, clean water is selected as a solvent and added to the reaction bottle, and then the separated liquid is added to the reaction bottle, and then extracted by a microwave extraction device, and separated after the extraction treatment to obtain an extract for standby; the temperature in the drying oven during the drying process is 65°C, and the drying time is 40 minutes, the amount of clean water used in the process of using clean water as a solvent is 400% of the mass fraction of the solid matter, and the microwave power of the microwave extraction device during the extraction process is 500W, and after pre-extraction for 1 minute, the power is increased to 1200W and continued for 10 minutes to complete the entire extraction process;

[0046] In the above step 5, the extract obtained in the above step 4 and the decoction obtained in the step 3 are put into a stirring device together, and then continuously stirred in the stirring device to obtain a mixed medicinal solution for standby use; the speed of continuous stirring is 30r / min, and the continuous stirring time is 18min;

[0047] In the above step 6, the mixed liquid medicine obtained in the above step 5 is placed in a concentration tank for reduced pressure concentration, and honey is added for seasoning after reduced pressure concentration, and Wuwutang oral liquid is obtained after seasoning for standby use; the evaporation rate of the concentration tank during the reduced pressure concentration process is 70kg / h, the steam pressure of the concentration tank is 0.15Mpa, and the vacuum degree in the concentration tank is -0.5mmHg; when honey is added for seasoning, the amount of honey added is 2% of the volume of the concentrated liquid;

[0048] Wherein in the above step seven, lactic acid bacteria and bifidobacterium are selected as the deployment of beneficial bacteria, and the two beneficial bacteria are cultured respectively. After the culture, the obtained lactic acid bacteria agent is mixed with the bifidobacterium agent in a 1:1 ratio, and the deployment agent is obtained after mixing for standby; in the process of culturing lactic acid bacteria, firstly, agar medium is selected for sterilization, and the pH value of the agar medium is adjusted to 6.5 after the sterilization, and fermentation culture is carried out in a temperature range of 35°C after inoculating lactic acid bacteria, and the pH value in the medium is always kept at 6.5 during the fermentation culture process, and the medium needs to be stirred at a low speed at a stirring rate of 5r / min during the fermentation culture process, and the lactic acid bacteria agent is obtained by centrifugal filtration after fermentation culture; in the process of culturing bifidobacterium, MRS medium is first selected as the culture medium liquid of bifidobacterium, and then the MRS medium is dissolved in a phosphate buffer solution with a pH value of 6.0, and then the culture liquid is placed in a flask for sterilization, and after inoculating bifidobacterium, constant temperature culture is carried out in a water bath at 37°C for 1200min, and the bifidobacterium agent is obtained by centrifugal filtration after constant temperature culture;

[0049] In the above step eight, the prepared bacterial agent obtained in the above step eight is added to the Wuwu Decoction oral liquid prepared in step six, and then mixed and stirred to obtain a mixed stock solution for standby use; when the prepared bacterial agent is added to the Wuwu Decoction oral liquid, the amount of the prepared bacterial agent added is 0.5% of the volume of the Wuwu Decoction oral liquid; the speed during the mixing and stirring process is 30r / min, and the mixing and stirring time is 18min;

[0050] In the above step nine, the mixed stock solution obtained in the above step eight is placed in a glass bottle, which is then sealed to obtain the Huangqi Guizhi Wuwu Decoction with added probiotics, which is then stored in a cool place.

[0051] Embodiment 3:

[0052] A probiotic-added Huangqi Guizhi Wuwu Decoction comprises: Huangqi, Guizhi, Paeonia lactiflora, ginger, jujube, Chinese angelica, Chuanxiong and safflower; the weight proportions of the components are: 10 parts of Huangqi, 8 parts of Guizhi, 10 parts of Paeonia lactiflora, 16 parts of ginger, 20 parts of jujube, 2 parts of Chinese angelica, 7 parts of Chuanxiong and 3 parts of safflower.

[0053] A processing method of Huangqi Guizhi Wuwu Decoction with added probiotics comprises the following steps: step 1, weighing raw materials; step 2, treating and soaking; step 3, decocting; step 4, microwave extraction; step 5, mixing medicinal liquids; step 6, concentrating and seasoning; step 7, preparing bacterial agents; step 8, adding beneficial bacteria; step 9, filling and storing;

[0054] In the above step 1, 10 parts of astragalus, 8 parts of cassia twig, 10 parts of peony, 16 parts of ginger, 20 parts of jujube, 2 parts of angelica, 7 parts of ligusticum chuanxiong and 3 parts of safflower are weighed according to the weight of each group and set aside;

[0055] In the above step 2, the astragalus, cinnamon twig, peony, ginger, jujube, angelica, chuanxiong and safflower prepared in the above step 1 are pretreated respectively, and after the pretreatment, they are placed in a soaking basin and soaked with clean water; the pretreatment of astragalus requires washing the dust remaining on the surface with clean water, and then slicing; the pretreatment of cinnamon twig requires washing the dust remaining on the surface with clean water, and then using a crusher to crush it; the pretreatment of peony requires washing the dust remaining on the surface with clean water; the pretreatment of ginger requires The ginger is peeled and then sliced; the pretreatment of jujube requires washing the dust remaining on the surface with clean water, and then removing the core; the pretreatment of angelica and Chuanxiong requires washing the dust remaining on the surface with clean water; the pretreatment of safflower requires gently washing with water through a sieve; the soaking treatment requires putting the pretreated astragalus, cassia twig, peony, ginger, jujube, angelica, Chuanxiong and safflower into a soaking basin together, and the amount of clean water added is 10 cm above the top of the medicinal materials, and the soaking time is 30 minutes;

[0056] In the above step 3, all the contents in the soaking basin in the above step 2 are poured into the ceramic pot, and then the liquid in the ceramic pot is boiled over high heat, and then the high heat is maintained for continuous boiling, and then the heat is reduced for continuous decoction, and after continuous decoction, the decoction residue and the decoction liquid are obtained by filtering, and the decoction residue and the decoction liquid are respectively set aside; the duration of continuous boiling is 5 minutes, and the duration of continuous decoction after reducing the heat is 45 minutes;

[0057] In the above step 4, the decocted medicinal residue prepared in the above step 3 is extruded, and the separated liquid obtained after the extrusion treatment is reserved, and the decocted medicinal residue after the extrusion treatment is placed in a drying oven for drying treatment, and then crushed by a pulverizer, and after crushing, clean water is selected as a solvent and added to the reaction bottle, and then the separated liquid is added to the reaction bottle, and then extracted by a microwave extraction device, and separated after the extraction treatment to obtain an extract for use; the temperature in the drying oven during the drying process is 70°C, and the drying time is 40 minutes, the amount of clean water used in the process of using clean water as a solvent is 450% of the mass fraction of the solid matter, the microwave power of the microwave extraction device during the extraction process is 500W, and after 2 minutes of pre-extraction, the power is increased to 1200W for 15 minutes to complete the entire extraction process;

[0058] In the above step 5, the extract obtained in the above step 4 and the decoction obtained in the step 3 are put into a stirring device together, and then continuously stirred in the stirring device to obtain a mixed medicinal solution for standby use; the speed of continuous stirring is 30r / min, and the continuous stirring time is 25min;

[0059] In the above step 6, the mixed liquid medicine obtained in the above step 5 is placed in a concentration tank for vacuum concentration, and honey is added for seasoning after vacuum concentration, and Wuwutang oral liquid is obtained after seasoning for standby use; the evaporation rate of the concentration tank during the vacuum concentration process is 70kg / h, the steam pressure of the concentration tank is 0.15Mpa, and the vacuum degree in the concentration tank is -0.08mmHg; when honey is added for seasoning, the amount of honey added is 2% of the volume of the concentrated liquid;

[0060] Wherein in the above step seven, lactic acid bacteria and bifidobacterium are selected as the deployment of beneficial bacteria, and the two beneficial bacteria are cultured respectively. After the culture, the obtained lactic acid bacteria agent is mixed with the bifidobacterium agent in a 1:1 ratio, and the deployment agent is obtained after mixing for standby; in the process of culturing lactic acid bacteria, firstly, agar medium is selected for sterilization, and the pH value of the agar medium is adjusted to 7.0 after the sterilization, and fermentation culture is carried out in a temperature range of 35°C after inoculating lactic acid bacteria, and the pH value in the medium is always kept at 7.0 during the fermentation culture process, and the medium needs to be stirred at a low speed at a stirring rate of 5r / min during the fermentation culture process, and the lactic acid bacteria agent is obtained by centrifugal filtration after fermentation culture; in the process of culturing bifidobacterium, MRS medium is first selected as the culture medium liquid of bifidobacterium, and then the MRS medium is dissolved in a phosphate buffer solution with a pH value of 7.0, and then the culture liquid is placed in a flask for sterilization, and after inoculating bifidobacterium, constant temperature culture is carried out in a water bath at 37°C for 1800min, and the bifidobacterium agent is obtained by centrifugal filtration after constant temperature culture;

[0061] In the above step eight, the prepared bacterial agent obtained in the above step eight is added to the Wuwu Decoction oral liquid prepared in step six, and then mixed and stirred to obtain a mixed stock solution for standby use; when the prepared bacterial agent is added to the Wuwu Decoction oral liquid, the amount of the prepared bacterial agent added is 0.5% of the volume of the Wuwu Decoction oral liquid; the speed during the mixing and stirring process is 60r / min, and the mixing and stirring time is 25min;

[0062] In the above step nine, the mixed stock solution obtained in the above step eight is placed in a glass bottle, which is then sealed to obtain the Huangqi Guizhi Wuwu Decoction with added probiotics, which is then stored in a cool place.

[0063] The performance of the Huangqi Guizhi Wuwu Decoction with added probiotics obtained in the above examples was tested respectively, and compared with the Huangqi Guizhi Wuwu Decoction on the market. The results are shown in the following table:

[0064] Residue of drug residue Vitamin B12 Content Flavonoid content Example 0.18mg / ml 0.0025mg / ml 0.0189mg / ml Comparative Example 1 0.83mg / ml 0.0023mg / ml 0.0185mg / ml Comparative Example 2 0.16mg / ml 0.0071mg / ml 0.0179mg / ml Comparative Example 3 0.22mg / ml 0.0026mg / ml 0.00125mg / ml

[0065] Based on the above, the advantages of the present invention are that, on the basis of the traditional formula, angelica, chuanxiong and safflower are added as auxiliary drugs, which can increase the content of trace elements such as vitamin B12, iron, zinc, etc. in the medicine after being mixed and decocted with the raw materials of the five-ingredient soup, help neutralize free radicals, reduce oxidative stress, and have anti-inflammatory properties, so that the medicine has the effect of promoting blood circulation and removing blood stasis, and enriches the medicinal efficacy of the traditional five-ingredient soup; by using microwave extraction to replace the two decoctions in the traditional preparation process, the rupture of the cell wall of the medicinal material is promoted, the dissolution of the effective ingredients is accelerated, the extraction efficiency is improved, and the waste of the efficacy is avoided; by adding a reduced pressure concentration process after preparation, the astragalus and cinnamon twig five-ingredient soup is prepared into an oral liquid, and then the prepared bifidobacterium agent and lactic acid bacteria agent are mixed and added to the oral liquid, and probiotics are added to the five-ingredient soup. After taking it, the beneficial bacteria can be colonized in the intestinal tract and reproductive system of the human body, which can produce definite health effects and improve the host microecological balance.

[0066] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A Huangqi Guizhi Wuwu Decoction with added probiotics, the formula comprising: Astragalus, cinnamon twig, peony, ginger, jujube, angelica, Chuanxiong and safflower; the characteristics are that the weight proportions of each component are: 8-10 parts of astragalus, 8-10 parts of cinnamon twig, 8-10 parts of peony, 16-20 parts of ginger, 16-20 parts of jujube, 2-3 parts of angelica, 5-7 parts of Chuanxiong and 3-5 parts of safflower.

2. A method for processing Huangqi Guizhi Wuwu Decoction with added probiotics, comprising the following steps: Step 1, weighing raw materials; Step 2, soaking; Step 3, boiling; Step 4, microwave extraction; Step 5, mixing medicinal liquids; Step 6, concentrating and seasoning; Step 7, preparing bacterial agents; Step 8, adding beneficial bacteria; Step 9, filling and storing; It is characterized by: In the above step 1, 8-10 parts of astragalus, 8-10 parts of cassia twig, 8-10 parts of peony, 16-20 parts of ginger, 16-20 parts of jujube, 2-3 parts of angelica, 5-7 parts of chuanxiong and 3-5 parts of safflower are weighed according to the weight of each group and set aside; In the above step 2, the astragalus, cinnamon twig, peony root, ginger, jujube, angelica, ligusticum chuanxiong and safflower prepared in the above step 1 are pre-treated respectively, and after the pre-treatment, they are placed in a soaking basin and soaked in clean water; In the above step 3, all the contents in the soaking basin in the above step 2 are poured into the ceramic pot, and then the liquid in the ceramic pot is boiled over high heat, and then the high heat is maintained to continue boiling, and then the heat is reduced to continue decocting, and after continuous decocting, the decocted medicinal residue and the decoction are filtered to obtain the decocted medicinal residue and the decoction liquid, which are set aside; In the above step 4, the decocted medicinal residue prepared in the above step 3 is subjected to extrusion treatment, and the separated liquid obtained after the extrusion treatment is used for standby, and the decocted medicinal residue after the extrusion treatment is placed in a drying oven for drying treatment, and then crushed by a pulverizer, and then water is selected as a solvent and added to the reaction bottle, and then the separated liquid is added to the reaction bottle, and then a microwave extraction device is used for extraction treatment, and after the extraction treatment, the extraction liquid is separated to obtain a standby extract; In the above step 5, the extract obtained in the above step 4 and the decoction obtained in the step 3 are put into a stirring device together, and then continuously stirred in the stirring device to obtain a mixed medicinal solution for standby use; In the above step 6, the mixed liquid medicine obtained in the above step 5 is placed in a concentration tank for reduced pressure concentration, and honey is added for seasoning after reduced pressure concentration, and the Wuwu Decoction oral liquid is obtained after seasoning for standby use; In the above step 7, lactic acid bacteria and bifidobacteria are selected as the prepared beneficial bacteria, and the two beneficial bacteria are cultured respectively, and the obtained lactic acid bacteria agent and bifidobacterium agent are mixed in a ratio of 1:1 after the culture, and the prepared bacterial agent is obtained after the mixing, and is set aside; In the above step eight, the prepared bacterial agent obtained in the above step eight is added to the Wuwu Decoction oral liquid prepared in step six, and then mixed and stirred to obtain a mixed stock solution for standby use; In the above step nine, the mixed stock solution obtained in the above step eight is placed in a glass bottle, which is then sealed to obtain the Huangqi Guizhi Wuwu Decoction with added probiotics, which is then stored in a cool place.

3. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 2, the pretreatment of Astragalus requires washing the dust remaining on the surface with clean water, and then slicing; the pretreatment of Cinnamon Twig requires washing the dust remaining on the surface with clean water, and then using a grinder to crush; the pretreatment of Paeonia lactiflora requires washing the dust remaining on the surface with clean water; The pretreatment of ginger requires peeling the ginger and then slicing it; the pretreatment of jujube requires washing the dust remaining on the surface with clean water, and then removing the core; the pretreatment of angelica and Chuanxiong requires washing the dust remaining on the surface with clean water; the pretreatment of safflower requires gently rinsing with water through a sieve; the soaking treatment requires putting the pretreated astragalus, cinnamon twig, peony, ginger, jujube, angelica, Chuanxiong and safflower into a soaking basin together, and the amount of clean water added is 5 to 10 cm above the top of the medicinal materials, and the soaking treatment time is 30 to 60 minutes.

4. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 3, the duration of continuous boiling is 3 to 5 minutes, and the duration of continuous decocting after turning to low heat is 45 to 60 minutes.

5. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 4, the temperature in the drying oven during the drying process is 65-70° C., and the drying time is 40-50 minutes. The amount of clean water used in the process of using clean water as a solvent is 400%-450% of the mass fraction of the solid matter. The microwave power of the microwave extraction device during the extraction process is 500-1000W. After pre-extraction for 1-2 minutes, the power is increased to 1200-1300W and continued for 10-15 minutes to complete the entire extraction process.

6. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 5, the rotation speed of continuous stirring is 30 to 60 r / min, and the continuous stirring time is 18 to 25 min.

7. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step six, the evaporation rate of the concentration tank during the reduced pressure concentration process is 70 to 350 kg / h, the steam pressure of the concentration tank is 0.15 MPa, and the vacuum degree in the concentration tank is -0.5 to -0.08 mmHg; when honey is added for seasoning, the amount of honey added is 2% to 5% of the volume of the concentrated liquid.

8. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 7, during the culture of lactic acid bacteria, an agar medium is first selected for sterilization, and the pH value of the agar medium is adjusted to 6.5-7.0 after the sterilization. After the lactic acid bacteria are inoculated, fermentation culture is carried out in a temperature range of 35-37° C. During the fermentation culture, the pH value of the culture medium is always maintained at 6.5-7.0, and the culture medium needs to be stirred at a low speed at a stirring rate of 5-8 r / min during the fermentation culture. After the fermentation culture, the lactic acid bacteria agent is obtained by centrifugal filtration.

9. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step 7, during the culturing of bifidobacteria, firstly, MRS medium is selected as the culture medium of bifidobacteria, then the MRS medium is dissolved in a phosphate buffer solution with a pH value of 6.0 to 7.0, and then the culture liquid is placed in a flask for sterilization. After inoculating bifidobacteria, constant temperature culture is carried out in a water bath at 37 to 41° C. for 1200 to 1800 minutes, and after constant temperature culture, the bifidobacterium agent is obtained by centrifugal filtration.

10. The method for processing Huangqi Guizhi Wuwu Decoction with added probiotics according to claim 2, characterized in that: In the step eight, when the prepared bacterial agent is added to the Wuwu Decoction oral liquid, the added amount of the prepared bacterial agent is 0.5% to 0.8% of the volume of the Wuwu Decoction oral liquid; the rotation speed during the mixing and stirring process is 30 to 60 r / min, and the mixing and stirring time is 18 to 25 min.

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