Application of QTL (Quantitative Trait Loci) Qsdss.saas.6A in identifying wheat SDS (Sodium Dodecyl Sulfate) sedimentation value genotype and identification kit
By developing molecular markers at Qsdss.saas.6A on the short arm of wheat 6A chromosomes, and establishing a KASP reaction system, the complex and time-consuming problem of wheat quality evaluation and detection in the prior art was solved, and the rapid screening of wheat lines with high SDS sedimentation values was achieved, and breeding efficiency was improved.
CN119662899AActive Publication Date: 2025-03-21CROP RES INST SHANDONG ACAD OF AGRI SCI
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Patent Information
- Application Number
- CN202510187232.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-20
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2045-02-20
AI Technical Summary
Technical Problem
The prior art detection methods in wheat quality evaluation are complex, time-consuming and inefficient, and it is difficult to quickly screen wheat plants with high SDS sedimentation values, limiting the wheat breeding efficiency.
Method used
A closely linked molecular marker was developed through the QTL site Qsdss.saas.6A found on the short arm of wheat 6A chromosome, and a KASP reaction system was established to provide a kit for identifying the genotype of wheat SDS sedimentation value.
Benefits of technology
The rapid, accurate and high-throughput identification of the SDS sedimentation value of wheat grains can be achieved, which can significantly improve breeding efficiency and quickly screen wheat plants with high SDS sedimentation value.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the technical field of biology, and particularly relates to application of a QTL (Quantitative Trait Loci) Qsdss.saas.6A to identification of a wheat SDS (Sodium Dodecyl Sulfate) sedimentation value genotype and an identification kit. The QTL locus Qsdss.saas.6A is located on a short arm of a wheat 6A chromosome, the genetic interval is 28.14 cM to 48.75 cM, and corresponding molecular markers on the two sides of the QTL locus Qsdss.saas.6A are AX-110002278 and AX-111581648. Meanwhile, the invention also provides a KASP primer group, the detection kit prepared from the KASP primer group is applied to detection of the SDS sedimentation value of wheat grains, wheat strains with high SDS sedimentation value can be rapidly screened, and the breeding efficiency is greatly improved; the identification method provided by the invention is simple in steps, reliable and practical, has an important application value in wheat breeding, and has a wide application prospect.
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