Use of a composition for fat reduction and oral cosmetic function

Abstract

The application discloses application of a composition in fat reduction and oral cosmetic function, and provides application of the composition in oral cosmetic function, and the composition comprises MN-Gup bacteria powder, galacto-oligosaccharide and white kidney bean extract, and specifically, the mass ratio of the MN-Gup bacteria powder, the galacto-oligosaccharide and the white kidney bean extract is 1:0.5-10:0.05-1. The application also provides application of the composition in fat reduction, and it is verified that the MN-Gup bacteria powder, the galacto-oligosaccharide and the white kidney bean extract in a specific ratio can be mutually matched to have a synergistic effect in fat reduction, such as promoting fat decomposition, inhibiting fat absorption and promoting energy metabolism, and oral cosmetics, such as lightening skin color and resisting photoaging.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fat reduction and oral cosmetology, in particular to the application of a composition in fat reduction and oral cosmetology. BACKGROUND

[0002] Obesity is a disease caused by excessive accumulation of fat in the body, which leads to an increase in body weight. It is related to eating habits, drugs, reduced exercise, and intestinal flora imbalance. Current treatments for obesity include drug therapy, increased exercise, and surgical treatment, which have unsatisfactory effects or adverse side effects.

[0003] In addition, as people's work and life pace accelerates, mental stress is also increasing, combined with unhealthy lifestyle, poor environment, ultraviolet rays and many other external factors, more and more people are facing skin problems. Prolonged exposure to ultraviolet light can cause skin damage, skin photoaging, and even skin cancer and other skin lesions. And because of skin oxidation, hormone level changes, ultraviolet radiation, damaged skin barrier, etc. causing pigmentation. Now for photoaging, pigmentation and other skin problems, using skin care products, masks and various medical treatments are mainly used, but they often cannot fundamentally change the biological process of skin aging, and there are a series of potential side effects such as hormone imbalance.

[0004] Oral cosmetology is a direction that has attracted much attention in the market in recent years. Oral cosmetology can improve the body's metabolism from the inside by ingesting bioactive ingredients, promote physical health and balance, and thus improve skin condition, rather than simply solve the external manifestations of skin problems. There are some compositions of natural ingredients that have fat-reducing or oral cosmetology effects, such as CN202310056093.9, which has a fat-reducing effect, and CN201710201371.X, which has an oral cosmetology effect. However, the main ingredients that produce the effects in these compositions are Chinese medicine ingredients, which are not suitable for certain groups to take due to taste and safety. SUMMARY

[0005] Therefore, the purpose of the present application is to provide a new composition that is more widely applicable to a wider range of people, which has both fat-reducing and oral cosmetology effects.

[0006] The application of a composition in oral cosmetology products, the composition comprising: MN-Gup bacteria powder, galacto-oligosaccharides, white kidney bean extract.

[0007] The strain in the MN-Gup bacterial powder is animal Bifidobacterium lactis MN-Gup, which is provided by Mengniu High-tech Dairy Products (Beijing) Co., Ltd. and is from the "world longevity village" of Bama Longevity Village in Guangxi, and has a preservation number of CGMCC No. 15578, which was preserved in the China General Microbiological Culture Collection Center on April 10, 2018, at address No. 1, Beichen West Road, Yard 3, Beijing Chaoyang District, Microbiology Institute of Chinese Academy of Sciences, postal code: 100101;

[0008] Galacto-oligosaccharides (GOS) are purchased from Great Ocean Ingredients PTY LTD company;

[0009] The white kidney bean extract can be self-extracted or purchased from the market, for example, white kidney bean extract purchased from Yunnan Tianbao Huaba Biological Resource Development Co., Ltd.

[0010] The mass ratio of the MN-Gup bacterial powder, galacto-oligosaccharides, and white kidney bean extract is 1:0.5-10:0.05-1. For example, the mass ratio of the MN-Gup bacterial powder and galacto-oligosaccharides can be 1:0.5, 1:1, 1:3, 1:5, 1:7, 1:9, 1:10, or any ratio between the ratios not shown, and the mass ratio of the MN-Gup bacterial powder and white kidney bean extract can be 1:0.05, 1:0.1, 1:0.3, 1:0.5, 1:0.8, 1:1, or any ratio between the ratios not shown. Preferably, the mass ratio of the MN-Gup bacterial powder, galacto-oligosaccharides, and white kidney bean extract is 1:2-4:0.1-0.3.

[0011] The number of viable MN-Gup bacteria in the composition is 3×10 9 CFU / g or more. For example, the number of viable animal Bifidobacterium lactis MN-Gup in the composition can be 3×10 9 CFU / g, 4×10 9 CFU / g, 5×10 9 CFU / g, 6×10 9 CFU / g, 7×10 9 CFU / g, 8×10 9 CFU / g, 9×10 9 CFU / g, 10×10 9 CFU / g, 20×10 9 CFU / g, 30×10 9 CFU / g, 50×10 9 CFU / g, 80×10 9 CFU / g, 100×10 9 CFU / g, 150×109 CFU / g, 200 x 10 9 CFU / g, 250 x 10 9 CFU / g, 300 x 10 9 CFU / g, 350 x 10 9 CFU / g, 400 x 10 9 CFU / g, 450 x 10 9 CFU / g, 500 x 10 9 CFU / g, 600 x 10 9 CFU / g, 600 x 10 9 CFU / g, 600 x 10 9 CFU / g.

[0012] The oral cosmetic product is an oral cosmetic product for lightening skin color or / and an oral cosmetic product for anti-photoaging.

[0013] The application further discloses application of the composition in preparation of a fat-reducing product. Further, the composition reduces fat by promoting fat decomposition and energy metabolism. Further, the composition reduces fat by inhibiting fat absorption.

[0014] The technical scheme of the application has the following advantages:

[0015] 1. The application provides a new composition, specifically including MN-Gup bacteria powder, galacto-oligosaccharide and white kidney bean extract; the composition has the oral cosmetic effect of lightening skin color and anti-photoaging by cooperation of the MN-Gup bacteria powder, the galacto-oligosaccharide and the white kidney bean extract; and the composition also has the effect of reducing fat by promoting fat decomposition and / or energy metabolism and inhibiting fat absorption.

[0016] 2. The composition provided by the application further optimizes the ratio of the MN-Gup bacteria powder, the galacto-oligosaccharide and the white kidney bean extract to 1:0.5-10:0.05-1, and has synergistic effects in the oral cosmetic effects of lightening skin color and anti-photoaging by cooperation of the galacto-oligosaccharide, the animal bifidobacterium lactis MN-Gup and the white kidney bean extract; and also has synergistic effects in the effects of reducing fat by promoting fat decomposition and / or energy metabolism and inhibiting fat absorption.

[0017] 3. The composition provided by the application has simple components, is easy to obtain, is free of traditional Chinese medicine components, has high safety, is suitable for a wider population, has an easy-to-adjust taste and can be widely applied to various products for reducing fat and oral cosmetics. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed in the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0019] Figure 1 is a graph of experimental results of the fat decomposition promoting effect of the present application;

[0020] Figure 2 is a graph of experimental results of the fat absorption inhibiting effect of the present application;

[0021] Figure 3 is a graph of experimental results of the energy metabolism promoting effect of the present application;

[0022] Figure 4 is a graph of experimental results of the skin lightening effect of the present application;

[0023] Figure 5 is a graph of experimental results of the anti-photoaging effect of the present application. EMBODIMENT

[0024] The following examples are provided to better further understand the present application, and are not limited to the best mode, and do not limit the content and protection scope of the present application. Any person under the inspiration of the present application or the combination of the present application with other prior art features, any product same or similar to the present application, falls within the protection scope of the present application.

[0025] If the specific experimental steps or conditions are not indicated in the examples, the operation or conditions can be carried out according to the conventional experimental steps described in the literature in the art. If the reagents or instruments are not indicated by the manufacturer, they are conventional reagent products that can be obtained by purchase.

[0026] Example 1

[0027] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:0.5:0.05.

[0028] The strain in the MN-Gup bacterial powder in the application is animal Bifidobacterium lactis MN-Gup, which is provided by Mengniu High-tech Dairy Products (Beijing) Co., Ltd., and is from the "world longevity village" Bama Longevity Village in Guangxi, China, with a preservation number of CGMCC No. 15578, which was preserved in the China General Microbiological Culture Collection Center on April 10, 2018, at No. 1, Beichen West Road, Haidian District, Beijing, China, at the Institute of Microbiology, Chinese Academy of Sciences, with a postcode of 100101. The viable bacterial content of the MN-Gup bacterial powder used in the experiment is 2x10 11 The galacto-oligosaccharide (GOS) used in the application is purchased from Great Ocean Ingredients PTY LTD company; and the white kidney bean extract (BYD) used in the application is purchased from Yunnan Tianbao Huaba Biological Resource Development Co., Ltd.

[0029] Example 2

[0030] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:3:0.2.

[0031] Example 3

[0032] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:10:1.

[0033] Example 4

[0034] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:0.1:0.01.

[0035] Example 5

[0036] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:100:10.

[0037] Example 6

[0038] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:10:0.05.

[0039] Example 7

[0040] A composition comprising MN-Gup bacterial powder, galacto-oligosaccharide, and white kidney bean extract at a mass ratio of 1:0.5:1.

[0041] Experimental Example

[0042] 1. Experimental animals

[0043] Zebrafish were all raised in fish water at 28℃ (water quality: 200 mg of instant sea salt was added to 1 L of reverse osmosis water, the conductivity was 450-550 μS / cm; the pH was 6.5-8.5; the hardness was 50-100 mg / L CaCO3).

[0044] 2. Test method

[0045] 2.1. Maximum test concentration (MTC) determination

[0046] MTC determination of fat decomposition promoting effect: 30 albino zebrafish of 2 dpf were randomly selected in each well (experimental group) of a 6-well plate. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup live bacteria concentration samples (concentrations are shown in Table 1) were respectively given to each well (experimental group) in water. A normal control group was set up, and the volume of each well was 3 mL. After 1 day of treatment at 28℃, Nile red dye was given to each experimental group in water. The treatment was continued at 28℃ until 4 dpf, and the MTC of the sample on normal zebrafish was determined.

[0047] MTC determination of fat absorption inhibiting effect: 30 wild type AB strain zebrafish of 5 days post-fertilization (5 dpf) were randomly selected in each beaker (experimental group). Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup live bacteria concentration samples (concentrations are shown in Table 2) were respectively given to each well (experimental group) in water. A normal control group and a model control group were set up, and the volume of each beaker was 20 mL. After 1 h of treatment at 28℃, chicken egg yolk powder was given to zebrafish in water in all concentration groups except the normal control group to establish a food fat absorption model. After 30 h of continuous treatment at 28℃, the samples were washed away, and the zebrafish were placed in the morning of 7 dpf. The MTC of the sample on model zebrafish was determined.

[0048] MTC determination of energy metabolism promoting effect: 30 wild type AB strain zebrafish of 6 hours post-fertilization (6 hpf) were randomly selected in each well (experimental group) of a 6-well plate. Different final concentrations of white kidney bean extract, different final concentrations of GOS, and different final concentrations of MN-Gup live bacteria concentration samples (concentrations are shown in Table 3) were respectively given to each well (experimental group) in water. A normal control group was set up, and the volume of each well was 3 mL. After 6 days of treatment at 28℃, the MTC of the sample on normal zebrafish was determined.

[0049] Skin lightening efficacy MTC determination: randomly selected 3 days after fertilization (3 dpf) melanin allele mutant zebrafish (Albino) in 6-well plates, 30 zebrafish per well (experimental group) were treated. Each well was respectively water-soluble to give different final concentrations of white kidney bean extract, different final concentrations of GOS, different final concentrations of MN-Gup live bacteria concentration samples (concentrations see Table 4), while setting normal control group and model control group, each well capacity was 3 mL. After 2 h of treatment at 28℃, except for the normal control group, the rest of the experimental groups were water-soluble to give menadione to establish a zebrafish pigmentation model. After 22 h of continuous treatment at 28℃, the MTC of the sample on the model zebrafish was determined.

[0050] Anti-photoaging efficacy MTC determination: randomly selected 3 days after fertilization (3 dpf) wild type AB strain zebrafish in 6-well plates, 30 zebrafish per well (experimental group) were treated. Each well was respectively water-soluble to give different final concentrations of white kidney bean extract, different final concentrations of GOS, different final concentrations of MN-Gup live bacteria concentration samples (concentrations see Table 5), while setting normal control group and model control group, each well capacity was 3 mL. After 3 h of treatment at 28℃, except for the normal control group, the rest of the experimental groups were simulated sunlight to establish a zebrafish tail fin wrinkle model. After 1 day of continuous treatment at 28℃, the MTC of the sample on the model zebrafish was determined.

[0051] 2.2, Evaluation of the effect of the composition on promoting fat decomposition

[0052] Randomly selected 2 dpf melanin allele mutant zebrafish (albino) in 6-well plates, 30 zebrafish per well (experimental group) were treated. Water-soluble to give the compound group samples of Examples 1-7, Examples 1-7 correspond to FP1~FP7 in turn, while setting normal control group (NC), model control group (MC), MN-Gup group, GOS group and BYD group, each well capacity was 3 mL, the total concentration of each group of intervention samples was 200 μg / mL. Each well capacity was 3 mL. After 1 day of treatment at 28℃, Nile red dye was water-soluble to each experimental group except the normal control group (NC). Continue to treat at 28℃ until 4 dpf, randomly select 10 zebrafish from each experimental group and place them under a fluorescence microscope for photography. Use NIS-Elements D 3.20 advanced image processing software to analyze and collect data, analyze the fluorescence intensity of zebrafish yolk sac fat, and statistically analyze the results of the index to evaluate the fat decomposition promoting efficacy of the sample. The statistical processing results are expressed as mean ± SE. Use SPSS 26.0 software for statistical analysis between two groups, p<0.05 indicates that the difference is statistically significant.

[0053] 2.3, Evaluation of the effect of the composition on inhibiting fat absorption

[0054] Randomly selected 5 dpf wild type AB strain zebrafish in beaker, each beaker (experimental group) treated 30 zebrafish. Water soluble administration of the complex group sample of example 2, named FP2, while setting normal control group (NC), model control group (MC), MN-Gup group, GOS group and BYD group, each hole capacity is 3 mL, the total concentration of each group of intervention samples is 200 μg / mL, each cup capacity is 20 mL. After 28℃ treatment for 1 h, except for the normal control group (NC), the rest of each concentration group was water soluble administration of chicken egg yolk powder to feed zebrafish to establish a food fat absorption model. After 28℃ continue to handle 30 h, wash away the sample, place 7 dpf in the morning to give oil red O for whole body fat staining, randomly select 10 zebrafish in each experimental group under the dissecting microscope and take pictures, use NIS-Elements D 3.20 advanced image processing software to collect data, analyze the fat staining intensity of the intestine and tail blood vessels, and evaluate the fat absorption inhibition efficacy of the sample according to the statistical analysis results of the index. The statistical processing results are expressed by mean ± SE. The statistical analysis between two groups was performed by SPSS 26.0 software, and p<0.05 indicated that the difference was statistically significant.

[0055] 2.4, evaluation of the effect of the composition for promoting energy metabolism

[0056] Randomly selected 6 hpf wild type AB strain zebrafish in 6 hole plate, each hole treated 30 zebrafish. Water soluble administration of the complex group sample of example 1-3, example 1-3 corresponds to FP1~FP3 in turn, while setting normal control group (NC), MN-Gup group, GOS group and BYD group, each hole capacity is 3 mL, the total concentration of each group of intervention samples is 200 μg / mL. After 28℃ treatment for 6 days, collect zebrafish samples according to coenzyme NAD(H) content detection kit instructions, use multifunctional enzyme label to detect zebrafish NAD+and NADH content, analyze the NAD+ / NADH ratio of each experimental group zebrafish, and evaluate the influence of sample on energy metabolism rate according to the statistical analysis results of the index. The statistical processing results are expressed by mean ± SE. Statistical analysis was performed by SPSS 26.0 software, and p<0.05 indicated that the difference was statistically significant.

[0057] 2.5, evaluation of the effect of the composition for brightening skin color

[0058] Randomly select 3 dpf melanin allele mutant Albino strain zebrafish in a 6-well plate, and treat 30 zebrafish in each well (experimental group). Water-soluble administration of the complex sample of Examples 1-7, Examples 1-7 correspond to FP1~FP7 in turn, while setting up a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group and a BYD group, with a capacity of 3 mL in each well, and the total concentration of the intervention sample in each group is 200 μg / mL. After 2 h of treatment at 28℃, the zebrafish pigment deposition model is established by water-soluble administration of menadione to all experimental groups except the normal control group, and the treatment is continued at 28℃ for 22 h. Randomly select 10 zebrafish from each experimental group and place them under a dissecting microscope for photography. Use NIS-Elements D 3.20 advanced image processing software to analyze and collect data, analyze the yolk sac pigment signal intensity of zebrafish, and evaluate the skin lightening efficacy of the sample based on the statistical analysis results of the index. The statistical processing results are expressed as mean ± SE, and the statistical analysis is performed using SPSS 26.0 software. p<0.05 indicates that the difference is statistically significant.

[0059] 2.6, Evaluation of the effect of the composition on anti-photoaging

[0060] Randomly select 3 dpf wild type AB strain zebrafish in a 6-well plate, and treat 30 zebrafish in each well. Water-soluble administration of the complex sample of Examples 1-3, Examples 1-3 correspond to FP1~FP3 in turn, while setting up a normal control group (NC), a model control group (MC), an MN-Gup group, a GOS group and a BYD group, with a capacity of 3 mL in each well, and the total concentration of the intervention sample in each group is 200 μg / mL. After 3 h of treatment at 28℃, the zebrafish tail fin wrinkle model is established by simulating sunlight irradiation in all experimental groups except the normal control group. Continue the treatment at 28℃ for 1 day. Randomly select 10 zebrafish from each experimental group and place them under a dissecting microscope for photography. Use NIS-Elements D 3.20 advanced image processing software to analyze and collect data, analyze the zebrafish tail fin area, and evaluate the anti-wrinkle efficacy of the sample based on the statistical analysis results of the index. The statistical processing results are expressed as mean ± SE. The statistical analysis is performed using SPSS 26.0 software. p<0.05 indicates that the difference is statistically significant.

[0061] 3, Experimental results

[0062] 3.1, Maximum detection concentration (MTC) determination results, as shown in Tables 1-5 below.

[0063] Table 1

[0064]

[0065] Table 2

[0066]

[0067] Table 3

[0068]

[0069] Table 4

[0070]

[0071] Table 5

[0072]

[0073] The zebrafish in each efficacy experiment group of white kidney bean extract in the intervention range of 12.5-200 μg / mL was similar to the state of the normal control group, so under the experimental conditions, the MTC of white kidney bean extract in promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, lightening skin color, and anti-photoaging efficacy was 200 μg / mL. Galactooligosaccharide (GOS) in the intervention range of 125-2000 μg / mL, the state of the zebrafish in the experimental group was similar to the state of the normal control group, so under the experimental conditions, the MTC of galactooligosaccharide in promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, lightening skin color, and anti-photoaging efficacy was 2000 μg / mL. MN-Gup bacterial powder in the intervention dose range of 1×10 5 CFU / mL-1×10 8 CFU / mL, the state of the zebrafish in the experimental group was similar to the state of the normal control group, while the 1×10 9 CFU / mL group appeared death in promoting fat decomposition, inhibiting fat absorption, promoting energy metabolism, lightening skin color, and anti-photoaging 5 efficacies, and the mortality rates were 17%, 100%, 80%, 13%, and 40%, respectively, so the MTC of MN-Gup bacterial powder in each efficacy under the experimental conditions was 1×10 8 CFU / mL.

[0074] 3.2, the experimental results of the composition in promoting fat decomposition are shown in Table 5. Figure 1

[0075] Figure 1 Among them, *: there was a significant difference (P<0.05) compared with the normal control group (NC); #: there was a significant difference (P<0.05) compared with the MN-Gup group; &: there was a significant difference (P<0.05) compared with the GOS group; ¥: there was a significant difference (P<0.05) compared with the BYD group.

[0076] Figure 1 ​​The fluorescence intensity of yolk sac fat in the NC group was 4.75 ± 230,000 pixels, while that in the MN-Gup, GOS, and BYD groups were 3.45 ± 330,000 pixels, 3.24 ± 190,000 pixels, and 3.27 ± 220,000 pixels, respectively. The MN-Gup, GOS, and BYD groups showed significant differences compared to the NC group, indicating that each of the MN-Gup, GOS, and BYD components promoted fat breakdown. In the compound groups, the fluorescence intensities of yolk sac fat in the FP1–FP7 groups were 2.63 ± 130,000 pixels, 2.55 ± 150,000 pixels, 2.53 ± 160,000 pixels, 3.75 ± 190,000 pixels, 3.12 ± 180,000 pixels, 2.54 ± 140,000 pixels, and 2.63 ± 150,000 pixels, respectively. The fluorescence intensity of yolk sac fat in groups FP1-FP7 was significantly lower than that in group NC, indicating that the composition of this invention promotes fat decomposition. Moreover, groups FP1-FP3 and FP6-FP7 showed significant differences compared with groups MN-Gup, GOS, and BYD, indicating that the composition composed of MN-Gup bacterial powder, galactooligosaccharides, and white kidney bean extract in a mass ratio of 1:0.5-10:0.05-1 has a synergistic effect in promoting fat decomposition.

[0077] 3.3 Experimental results on the composition's inhibition of fat absorption are as follows: Figure 2 As shown.

[0078] Figure 2 In the mean squares, * indicates a significant difference compared to the model control group (MC) (P < 0.05); # indicates a significant difference compared to the MN-Gup group (P < 0.05); & indicates a significant difference compared to the GOS group (P < 0.05); and ￥ indicates a significant difference compared to the BYD group (P < 0.05).

[0079] pass Figure 2 The staining intensity of intestinal and tail vessel fat in the NC group was 25,700 ± 0.08 megapixels, while that in the MC group was 92,100 ± 0.29 megapixels. The MC group was significantly higher than the NC group, indicating successful model establishment. The staining intensity of intestinal and tail vessel fat in the MN-Gup, GOS, and BYD groups was 78,800 ± 0.32 megapixels, 72,800 ± 0.21 megapixels, and 70,900 ± 0.30 megapixels, respectively, significantly lower than that in the MC group, indicating that the three single components had an inhibitory effect on fat absorption. The staining intensity of intestinal and tail vessel fat in the FP2 group was 62,000 ± 0.28 megapixels, significantly lower than that in the MC group, and also significantly lower than that in the MN-Gup, GOS, and BYD groups. This indicates that the composition of MN-Gup bacterial powder, galactooligosaccharides, and white kidney bean extract in a mass ratio of 1:2~4:0.1~0.3 has a synergistic effect in inhibiting fat absorption.

[0080] 3.4 Experimental results on the composition's promotion of energy metabolism are as follows:Figure 3 As shown in Table 2.

[0081] Figure 3 Among them, *: there is a significant difference (P<0.05) compared with the normal control group (NC); #: there is a significant difference (P<0.05) compared with the MN-Gup group; &: there is a significant difference (P<0.05) compared with the GOS group; ¥: there is a significant difference (P<0.05) compared with the BYD group.

[0082] As shown in Table 2. Figure 3 It can be seen that the NAD + / NADH ratio of the NC group was 1.17±0.09, and the NAD + / NADH ratios of the MN-Gup group, the GOS group and the BYD group were 1.69±0.10, 1.30±0.07 and 1.32±0.12 respectively, and the NAD + / NADH ratio of the FP1~FP3 group was 2.62±0.12, 2.18±0.06 and 2.04±0.02 respectively, which were significantly higher than that of the NC group, indicating that FP1~FP3 had the effect of promoting energy metabolism. Moreover, the NAD + / NADH ratios of the FP1~FP3 group were significantly higher than those of the MN-Gup group, the GOS group and the BYD group, indicating that the three components had a synergistic effect in promoting energy metabolism within the ratio range of 1:0.5~10:0.05~1.

[0083] 3.5, the experimental results of the skin lightening effect of the composition are shown in Table 3. Figure 4

[0084] Figure 4 Among them, *: there is a significant difference (P<0.05) compared with the model control group (MC); #: there is a significant difference (P<0.05) compared with the MN-Gup group; &: there is a significant difference (P<0.05) compared with the GOS group; ¥: there is a significant difference (P<0.05) compared with the BYD group.

[0085] As shown in Table 2. Figure 4 ​It can be known that the pigment signal intensity of the yolk sac of the NC group is 24900±900 pixels, and the MC group is 37200±1600 pixels. The pigment signal intensity of the yolk sac of the MC group is significantly higher than that of the NC group, which indicates that the modeling is successful. The pigment signal intensity of the yolk sac of the MN-Gup group, the GOS group and the BYD group is 27500±1100 pixels, 29800±1000 pixels and 30300±1100 pixels respectively, which is significantly lower than that of the MC group, which indicates that the MN-Gup, GOS and BYD single components all have the effect of brightening skin color. In the compound group, the pigment signal intensity of the yolk sac of the FP1~FP7 group is 19500±1100 pixels, 20000±500 pixels, 24300±600 pixels, 29900±1000 pixels, 29600±1200 pixels, 23100±700 pixels and 20000±900 pixels respectively. The pigment signal intensity of the yolk sac of the FP1~FP7 group is significantly lower than that of the MC group, which indicates that the composition in the application has the effect of brightening skin color. Among them, FP1~FP3 and FP6~FP7 have significant differences compared with the MN-Gup group, the GOS group and the BYD group, which indicates that the composition composed of MN-Gup, galacto-oligosaccharide and white kidney bean extract with a mass ratio of 1:0.5~10:0.05~1 has a synergistic effect on brightening skin color.

[0086] 3.6, the experimental results of the anti-photoaging effect of the composition are shown in Figure 5

[0087] Figure 5 In the table, *: there is a significant difference (P<0.05) compared with the model control group (MC); #: there is a significant difference (P<0.05) compared with the MN-Gup group; &: there is a significant difference (P<0.05) compared with the GOS group; ¥: there is a significant difference (P<0.05) compared with the BYD group.

[0088] By Figure 5 ​It can be seen that the tail fin area of the NC group is 79.87±1.00 million pixels, and the tail fin area of the MC group is 56.56±1.36 million pixels. The tail fin area of the MC group is significantly lower than that of the NC group, indicating that the simulation of sunlight causes the shrinkage of the zebrafish tail fin, and the modeling is successful. The tail fin areas of the MN-Gup group, the GOS group and the BYD group are 64.42±1.49 million pixels, 63.65±1.55 million pixels and 62.37±0.83 million pixels, respectively, which are significantly higher than that of the MC group, indicating that MN-Gup, GOS and BYD single components all have the effect of resisting photoaging. In the compound group, the tail fin areas of FP1~FP3 groups are 70.24±1.47 million pixels, 72.11±1.84 million pixels and 68.84±0.86 million pixels, respectively. The tail fin areas of FP1~FP3 groups are significantly higher than that of the MC group, indicating that they have the effect of resisting photoaging. Moreover, compared with the MN-Gup group, the GOS group and the BYD group, there are significant differences, indicating that the FP1~FP3 groups have a synergistic effect on resisting photoaging within the range of the mass ratio of 1:0.5~10:0.05~1.

[0089] Obviously, the above embodiments are only examples for clearly illustrating but not limiting the embodiments. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. All the embodiments do not need to be exhausted, and the obvious changes or variations derived therefrom are still within the protection scope of the present application.

Claims

1. Use of a composition for the manufacture of an oral cosmetic product, characterized in that, The composition comprises: MN-Gup bacterial powder, galacto-oligosaccharide, white kidney bean extract; the mass ratio of the MN-Gup bacterial powder, galacto-oligosaccharide, white kidney bean extract is 1:0.5-10:0.05-1. The oral cosmetic product is an oral cosmetic product for lightening skin color or / and an oral cosmetic product for anti-photoaging.

2. Use according to claim 1, characterized in that, The viable cell count of MN-Gup bacteria in the composition is 3 x 10 9 CFU / g or more.

3. Use according to claim 2, characterized in that, The viable cell count of MN-Gup bacteria in the composition is 4 x 10 9 500 x 10 9 CFU / g.

4. Use according to any one of claims 1 to 3, characterized in that, The mass ratio of the MN-Gup bacterial powder, galacto-oligosaccharide, white kidney bean extract is 1:2-4:0.1-0.3.

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