Preparation method and application of dendrobium officinale extract

Abstract

The invention relates to a preparation method and application of a dendrobium officinale extract, and belongs to the technical field of plant extraction. Physical cracking of a cell structure is realized by adopting an ultralow-temperature crushing technology, and meanwhile, thermosensitive components are prevented from being degraded. A compound system of cellulase, pectinase and neutral protease is used for synergistic enzymolysis under the conditions that the temperature is 35-45 DEG C and the pH is 5-6, cell wall components are efficiently hydrolyzed, and combined anti-inflammatory active ingredients are released. Then carrying out anaerobic fermentation by adopting lactobacillus rhamnosus, hydrolyzing bibenzyl aglycones by virtue of beta-glucosidase secreted by thalli, promoting alkaloid protonation to form water-soluble salts by virtue of metabolite lactic acid, and meanwhile, forming a composite active component by virtue of exopolysaccharides and dendrobium polysaccharides; after the fermentation liquor is filtered, the fermentation liquor is compounded with ultra-filtration purified water extract obtained through ultrasonic-assisted extraction according to the ratio of (2-4): 1, and finally the high-purity extract is obtained through freeze drying. The extract is controllably released in gastrointestinal tracts, has an inhibition rate on LPS-induced inflammation superior to that of a traditional process, and can be used in the field of intestinal inflammation resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extraction and relates to a preparation method and application of a Dendrobium officinale extract. Background Art

[0002] Dendrobium officinale is a traditional precious Chinese medicinal material, rich in polysaccharides, bibenzyl compounds, alkaloids, amino acids and other active ingredients. It has multiple pharmacological effects such as nourishing yin and clearing heat, benefiting the stomach and promoting fluid production, and enhancing immunity. Existing studies have shown that it has antioxidant, anti-inflammatory, and immunomodulatory activities.

[0003] Existing plant extraction methods have certain limitations. Conventional methods such as water extraction and alcohol extraction have high extraction temperatures and long extraction times, which may cause degradation or inactivation of heat-sensitive active ingredients, affecting the biological activity of the final extract. Traditional methods generally pursue the yield of total polysaccharides or total extracts, and insufficiently enrich active components with specific biological activities, resulting in unclear active ingredients in the product and unstable effects. In addition, ingredients such as Dendrobium officinale polysaccharides may be degraded or incompletely absorbed in the upper gastrointestinal tract after oral administration, making it difficult for them to effectively reach and act on sites of intestinal inflammation.

[0004] Therefore, there is an urgent need to develop a preparation method and application of a Dendrobium officinale extract. Summary of the Invention

[0005] In view of the above-mentioned deficiencies in the prior art, the present invention provides a preparation method and application of a Dendrobium officinale extract.

[0006] The purpose of the present invention can be achieved through the following technical solutions: A preparation method of a Dendrobium officinale extract, wherein the specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology at a pulverization temperature of -80~-90°C for 60~90 s. Ultra-low temperature pulverization was carried out under high-purity nitrogen protection at a nitrogen flow rate of 0.5~1 L / min to obtain material A. S2: Place material A in PBS buffer solution so that the material-liquid ratio is 1:(10-20), perform enzymatic hydrolysis on material A at 35-45°C, add 5-10% of the weight of the composite enzyme preparation to the material A, perform enzymatic hydrolysis at a speed of 150-250 rpm for 4-6 h, and centrifuge to obtain enzymatic hydrolyzate B and enzymatic hydrolysis residue; S3: Adjust the pH of enzymatic hydrolysate B to 6.2-6.5, sterilize at 115°C for 15 min, cool to room temperature, inoculate with 5% activated Lactobacillus rhamnosus bacteria solution, incubate anaerobically at 37°C for 18-24 h, centrifuge at 4°C, and filter through a 0.22 μm filter to obtain fermentation supernatant C; S4: The enzymatic residue was extracted with pure water at a temperature of 50-60°C, a solid-liquid ratio of 1:(6-8), and an extraction time of 2-3 h. Ultrasonic assistance was also used. The extract was centrifuged and then filtered through a 10 μm + 2 μm fine filter membrane. The fine filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25-30°C to obtain a water extract D. S5: mixing the fermentation supernatant C and the water extract D, pre-freezing the mixture at -40°C for 4 hours, and freeze-drying the mixture for 20-24 hours to obtain the Dendrobium officinale extract.

[0007] As a preferred technical solution of the present invention, the particle size of the Dendrobium officinale after being treated with the ultra-low temperature pulverization technology in S1 is D50≤50 μm.

[0008] As a preferred technical solution of the present invention, the composite enzyme preparation in S2 is cellulase, pectinase, and neutral protease in a mass ratio of (1-3):1:1.

[0009] As a preferred technical solution of the present invention, the pH of the PBS buffer in S2 is 5-6.

[0010] As a preferred technical solution of the present invention, the number of viable bacteria in the activated bacterial solution of Lactobacillus rhamnosus in S3 is ≥10 9 CFU / mL.

[0011] As a preferred technical solution of the present invention, the centrifugal speed in S3 is 8000-9000 rpm, and the centrifugal time is 10-20 min.

[0012] As a preferred technical solution of the present invention, the ultrasonic frequency in S4 is 10-20 kHz, and the power density is 0.3-0.5 W / cm 2 .

[0013] As a preferred technical solution of the present invention, the fermentation supernatant C and the water extract D in S5 are mixed in a volume ratio of (2-4):1.

[0014] The invention discloses an application of a Dendrobium officinale extract, wherein the Dendrobium officinale extract can be applied to the field of intestinal anti-inflammatory.

[0015] The present invention first crushes Dendrobium officinale at -80 to -90°C, rapidly freezes the intracellular water with liquid nitrogen, and forms micron-sized ice crystals. The volume expansion effect of the ice crystals is then used to mechanically destroy the cell walls and cell membranes, thereby achieving physical dissociation of the cell structure. This process avoids the breakage of polysaccharide β-glycosidic bonds and oxidation of alkaloid nitrogen heterocyclic structures caused by frictional heat generated by traditional mechanical crushing. Nitrogen protection effectively inhibits enzymatic oxidation and non-enzymatic browning reactions of phenolic components. After crushing to D50 ≤ 50 μm, the specific surface area of ​​the material is significantly increased, thereby significantly increasing the contact probability between the substrate and the enzyme molecules in the enzymatic hydrolysis reaction.

[0016] A complex enzyme system consisting of cellulase, pectinase, and neutral protease in a specific mass ratio was developed. Cellulase efficiently hydrolyzes the β-1,4-glycosidic bonds of cell wall cellulose, disrupting the crystalline structure; pectinase degrades the α-1,4-galacturonic acid bonds of extracellular pectin, reducing cell adhesion; and neutral protease specifically degrades glycoproteins covalently bound to polysaccharides, releasing the bound polysaccharides and reducing the viscosity of the extract, thereby exposing or releasing the target anti-inflammatory active ingredients. Under conditions of 35-45°C and pH 5-6, the complex enzymatic hydrolysis efficiency was significantly improved compared to that of a single enzyme system, significantly increasing the release of target ingredients such as bibenzyls and alkaloids, while avoiding enzyme inactivation caused by high temperatures and the degradation of heat-sensitive dendrobium alkaloids.

[0017] After enzymatic hydrolysis, Lactobacillus rhamnosus is inoculated for anaerobic fermentation. The β-glucosidase produced by the bacteria can specifically hydrolyze the bibenzyl aglycones in Dendrobium officinale, releasing free anti-inflammatory active ingredients. The metabolite lactic acid lowers the pH of the system to 4.5-5.0, promoting the protonation of alkaloids and the formation of water-soluble salts. The extraction efficiency is significantly improved compared to ethanol extraction. The extracellular polysaccharides produced by fermentation form a complex with Dendrobium officinale polysaccharides, protecting the active ingredients from oxidation through steric hindrance. Filtration with a 0.22μm filter membrane can completely intercept the bacteria and large molecular impurities. Lactic acid in the fermentation broth forms a lactate complex with bibenzyl compounds, which decomposes after freeze-drying to release high-purity active ingredients.

[0018] Ultrasonic-assisted aqueous extraction disrupts incompletely enzymatically digested cell debris, releasing the arabinogalactan-protein complex bound to the cell wall. A 10μm filter initially retains larger particles, such as plant debris and incompletely pulverized cell clusters, while a 2μm filter further removes finer suspended solids, such as micron-sized colloidal particles. This graded filtration process reduces the load on a single filter membrane, extending its lifespan while also minimizing the risk of contamination in the subsequent ultrafiltration step and improving product clarity. Polysaccharide purity is further enhanced after ultrafiltration separation. Residual lactic acid in the aqueous extract promotes protonation of the polysaccharide, enhancing electrostatic interactions with the bibenzyl compound.

[0019] When the fermentation supernatant and aqueous extract are combined at a specific volume ratio, lactic acid and dendrobium polysaccharides form a pH-sensitive nanogel in a simulated gastrointestinal fluid, enabling controlled release of the active ingredient. Freeze-drying avoids the glass transition phenomenon present in liquid water through direct sublimation of ice crystals, preventing dehydration and condensation of the polysaccharide hydroxyl groups. Compared to hot air drying, the active ingredient retention rate after drying exceeds 98%.

[0020] Beneficial effects of the present invention: The present invention utilizes ultra-low temperature liquid nitrogen pulverization technology at -80-90°C, utilizing the ice crystal expansion effect to physically destroy the cell structure, preventing the breakage of polysaccharide β-glycosidic bonds and oxidation of alkaloids. Nitrogen protection inhibits phenolic browning, significantly increasing the specific surface area and significantly enhancing enzymatic contact efficiency. A complex enzyme system synergistically hydrolyzes the cell wall, extracellular matrix, and bound proteins. Enzymatic efficiency is improved at 35-45°C and pH 5-6, increasing the release of heat-sensitive components with a retention rate of >95%. β-glucosidase produced by fermentation of Lactobacillus rhamnosus hydrolyzes bibenzyl aglycones. The metabolite lactic acid promotes the protonation of alkaloids, resulting in high extraction efficiency and the formation of an exopolysaccharide-Dendrobium polysaccharide complex to protect the active ingredients. Ultrasound-assisted water extraction combined with ultrafiltration separation further improves the purity of the polysaccharide. The fermentation broth and water extract are mixed in a ratio of (2-4):1, enabling controlled release in the gastrointestinal tract. The preparation method of the present invention achieves efficient release, targeted enrichment, and synergistic enhancement of anti-inflammatory active ingredients. The extract significantly improves the inhibitory rate of the LPS-induced inflammation model compared to commercially available products. DETAILED DESCRIPTION

[0021] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0022] In the Examples and Comparative Examples of the present invention: Cellulase: purchased from Shanghai Yuanye Biotechnology Co., Ltd., derived from Trichoderma reesei ATCC26921; Pectinase: purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd., derived from Rhizopus; Neutral protease: purchased from Shanghai Yuanye Biotechnology Co., Ltd., derived from Bacillus subtilis; Lactobacillus rhamnosus LR22: provided by Shanghai Jiaotong University Onli Co., Ltd., accession number CNCM I-4474; 10 μm and 2 μm fine filter membranes: purchased from Merck Group, Germany; Ultrafiltration membrane: purchased from Shanghai Saiao Separation Technology Engineering Co., Ltd.

[0023] Example 1 A preparation method of a Dendrobium officinale extract, wherein the specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology at a pulverization temperature of -85°C for 75 seconds. Ultra-low temperature pulverization was carried out under high-purity nitrogen protection at a nitrogen flow rate of 0.8 L / min to obtain material A with a particle size of D50 ≤ 50 μm. S2: Material A was placed in a PBS buffer solution with a pH of 5.5 so that the material-liquid ratio was 1:15, and material A was enzymatically hydrolyzed at 40°C. A composite enzyme preparation was added at a weight ratio of 8% of material A, wherein the composite enzyme preparation was a mixture of cellulase, pectinase, and neutral protease in a weight ratio of 2:1:1. The mixture was enzymatically hydrolyzed at a speed of 200 rpm for 5 h, and centrifuged to obtain enzymatic hydrolyzate B and enzymatic hydrolysis residue; S3: Adjust the pH of enzymatic hydrolysate B to 6.3, sterilize at 115°C for 15 min, cool to room temperature, and inoculate with 5% activated Lactobacillus rhamnosus bacteria solution. The number of viable cells in the activated Lactobacillus rhamnosus bacteria solution should be ≥10 9 CFU / mL, placed in anaerobically cultured at 37°C for 20 h, then centrifuged at 4°C at a speed of 8500 rpm for 15 min, and then filtered through a 0.22 μm filter membrane to obtain the fermentation supernatant C; S4: The enzymatic residue was extracted with pure water at an extraction temperature of 55 °C, a solid-liquid ratio of 1:7, and an extraction time of 2.5 h. Ultrasonic assistance was also used at a frequency of 15 kHz and a power density of 0.4 W / cm 2 After sedimentation and centrifugation, the extract was filtered through a 10 μm + 2 μm fine filtration membrane. The fine filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 28 °C to obtain aqueous extract D. S5: The fermentation supernatant C and the water extract D were mixed in a volume ratio of 3:1, pre-frozen at -40°C for 4 hours, and freeze-dried for 22 hours to obtain the Dendrobium officinale extract.

[0024] The invention discloses an application of a Dendrobium officinale extract, wherein the Dendrobium officinale extract can be applied to the field of intestinal anti-inflammatory.

[0025] Example 2 A preparation method of a Dendrobium officinale extract, wherein the specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology at a pulverization temperature of -80°C for 60 seconds. Ultra-low temperature pulverization was carried out under high-purity nitrogen protection at a nitrogen flow rate of 0.5 L / min to obtain material A with a particle size of D50 ≤ 50 μm. S2: Material A was placed in a PBS buffer solution with a pH of 5 so that the material-liquid ratio was 1:10, and material A was enzymatically hydrolyzed at 35°C. A composite enzyme preparation was added at a weight ratio of 5% of material A, wherein the composite enzyme preparation was a mixture of cellulase, pectinase, and neutral protease in a weight ratio of 1:1:1. The material was enzymatically hydrolyzed at a speed of 150 rpm for 4 h, and the mixture was centrifuged to obtain enzymatic hydrolyzate B and enzymatic hydrolysis residue; S3: Adjust the pH of enzymatic hydrolysate B to 6.2, sterilize at 115°C for 15 min, cool to room temperature, and inoculate with 5% activated Lactobacillus rhamnosus bacteria solution. The number of viable cells in the activated Lactobacillus rhamnosus bacteria solution should be ≥10 9 CFU / mL, incubated anaerobically at 37°C for 18 h, then centrifuged at 4°C at 8000 rpm for 10 min, and then filtered through a 0.22 μm filter to obtain the fermentation supernatant C; S4: The enzymatic residue was extracted with pure water at a temperature of 50 °C, a solid-liquid ratio of 1:6, and an extraction time of 2 h. Ultrasonic assistance was also used at a frequency of 10 kHz and a power density of 0.3 W / cm 2 After sedimentation and centrifugation, the extract was filtered through a 10 μm + 2 μm fine filtration membrane. The fine filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25 °C to obtain aqueous extract D. S5: The fermentation supernatant C and the water extract D were mixed in a volume ratio of 2:1, pre-frozen at -40°C for 4 hours, and freeze-dried for 20 hours to obtain the Dendrobium officinale extract.

[0026] The invention discloses an application of a Dendrobium officinale extract, wherein the Dendrobium officinale extract can be applied to the field of intestinal anti-inflammatory.

[0027] Example 3 A preparation method of a Dendrobium officinale extract, wherein the specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology at a pulverization temperature of -90°C for 90 seconds. Ultra-low temperature pulverization was carried out under high-purity nitrogen protection at a nitrogen flow rate of 1 L / min to obtain material A with a particle size of D50 ≤ 50 μm. S2: Material A was placed in a PBS buffer solution with a pH of 6 so that the material-liquid ratio was 1:20, and material A was enzymatically hydrolyzed at 45°C. A composite enzyme preparation was added at a weight ratio of 10% of material A, wherein the composite enzyme preparation was a mixture of cellulase, pectinase, and neutral protease in a weight ratio of 3:1:1. The material was enzymatically hydrolyzed at a speed of 250 rpm for 6 h, and the mixture was centrifuged to obtain enzymatic hydrolyzate B and enzymatic hydrolysis residue. S3: Adjust the pH of enzymatic hydrolysate B to 6.5, sterilize at 115℃ for 15 min, cool to room temperature, and inoculate with 5% activated Lactobacillus rhamnosus bacteria solution. The number of viable cells in the activated Lactobacillus rhamnosus bacteria solution should be ≥10 9 CFU / mL, placed in anaerobically cultured at 37°C for 24 h, then centrifuged at 4°C at a speed of 9000 rpm for 20 min, and then filtered through a 0.22 μm filter membrane to obtain the fermentation supernatant C; S4: The enzymatic residue was extracted with pure water at an extraction temperature of 60 °C, a solid-liquid ratio of 1:8, and an extraction time of 3 h. Ultrasonic assistance was also used at a frequency of 20 kHz and a power density of 0.5 W / cm 2 After sedimentation and centrifugation, the extract was filtered through a 10 μm + 2 μm fine filtration membrane. The fine filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 30 °C to obtain aqueous extract D. S5: The fermentation supernatant C and the water extract D were mixed in a volume ratio of 4:1, pre-frozen at -40°C for 4 hours, and freeze-dried for 24 hours to obtain the Dendrobium officinale extract.

[0028] The invention discloses an application of a Dendrobium officinale extract, wherein the Dendrobium officinale extract can be applied to the field of intestinal anti-inflammatory.

[0029] Comparative Example 1 The complex enzyme preparation in S2 was replaced with an equal amount of cellulase, and the remaining steps were the same as in Example 1.

[0030] Comparative Example 2 The complex enzyme preparation in S2 was replaced with an equal amount of pectinase, and the remaining steps were the same as in Example 1.

[0031] Comparative Example 3 The complex enzyme preparation in S2 was replaced with an equal amount of neutral protease, and the remaining steps were consistent with Example 1.

[0032] Comparative Example 4 No activated Lactobacillus rhamnosus bacterial solution was added in S3, and the remaining steps were the same as those in Example 1.

[0033] Comparative Example 5 In S4, no ultrafiltration membrane filtration treatment is performed, and the remaining steps are the same as those in Example 1.

[0034] Comparative Example 6 In S4, the combined fine filtration through the 10 μm+2 μm fine filtration membrane is not performed, and the remaining steps are the same as those in Example 1.

[0035] Comparative Example 7 The freeze drying in S5 was replaced by vacuum drying at 70°C for 22 h, and the remaining steps were the same as in Example 1.

[0036] Anti-inflammatory activity test Experimental steps: Mouse mononuclear macrophage leukemia cells RAW264.7 were selected and 100 μL of 5×10 4 / mL of cells, cultured at 37 ° C, 5% CO2, and more than 90% humidity. After 24 hours, 50 μL of the prepared test compound solution was added and continued to be cultured under the same conditions. After 1 hour, 50 μL of the prepared LPS (final concentration 1 μg / mL) solution was added. After 24 hours, 100 μL of the supernatant was taken from each well and placed in a new 96-well plate. Then 100 μL of Griess reagent was added to each well and mixed by the cross-cross method. The absorbance of each well was measured and recorded at a wavelength of 540 nm on an enzyme reader, and the NO inhibition rate was calculated according to the following formula. The control group was a commercially available Dendrobium extract purchased from Wuhan Proloff Biotechnology Co., Ltd.; the negative control group was DMSO. The test compound was diluted 5 times and a half in a concentration gradient. The horizontal axis represents the concentration of the test compound, and the vertical axis represents the inhibition rate. The IC of the test compound was calculated. 50 value.

[0037] Inhibition rate (%) = (C2-C1) / (C2-C0) × 100%; Where: C0, C1, and C2 are the absorbance values ​​of the blank control group (without LPS), experimental group, and negative control group (with LPS) measured at 540 nm. Calculate the inhibition rate at each concentration, and then calculate the half-inhibitory concentration (IC50) of the extract on LPS-induced NO production in RAW264.7. 50 value).

[0038] The experimental data of the embodiments and comparative examples show that the Dendrobium officinale extract prepared in the present invention exhibits strong anti-inflammatory activity and has good application prospects in the field of intestinal anti-inflammatory.

[0039] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a Dendrobium officinale extract, characterized in that: The specific steps of the preparation method are as follows: S1: Fresh Dendrobium officinale strips were processed using ultra-low temperature pulverization technology at a pulverization temperature of -80~-90°C for 60~90 s. Ultra-low temperature pulverization was carried out under high-purity nitrogen protection at a nitrogen flow rate of 0.5~1 L / min to obtain material A. S2: Place material A in PBS buffer solution so that the material-liquid ratio is 1:(10-20), perform enzymatic hydrolysis on material A at 35-45°C, add 5-10% of the weight of the composite enzyme preparation to the material A, perform enzymatic hydrolysis at a speed of 150-250 rpm for 4-6 h, and centrifuge to obtain enzymatic hydrolyzate B and enzymatic hydrolysis residue; S3: Adjust the pH of enzymatic hydrolysate B to 6.2-6.5, sterilize at 115°C for 15 min, cool to room temperature, inoculate with 5% activated Lactobacillus rhamnosus bacteria solution, incubate anaerobically at 37°C for 18-24 h, centrifuge at 4°C, and filter through a 0.22 μm filter to obtain fermentation supernatant C; S4: The enzymatic residue was extracted with pure water at a temperature of 50-60°C, a solid-liquid ratio of 1:(6-8), and an extraction time of 2-3 h. Ultrasonic assistance was also used. The extract was centrifuged and then filtered through a 10 μm + 2 μm fine filter membrane. The fine filtrate was then filtered through an ultrafiltration membrane (100 kDa) at an operating pressure of 0.1 MPa and a temperature of 25-30°C to obtain a water extract D. S5: mixing the fermentation supernatant C and the water extract D, pre-freezing the mixture at -40°C for 4 hours, and freeze-drying the mixture for 20-24 hours to obtain the Dendrobium officinale extract.

2. The method for preparing a Dendrobium officinale extract according to claim 1, wherein The particle size of the Dendrobium officinale after being processed by the ultra-low temperature pulverization technology in S1 is D50≤50 μm.

3. The method for preparing a Dendrobium officinale extract according to claim 1, wherein The complex enzyme preparation in S2 is cellulase, pectinase, and neutral protease in a mass ratio of (1-3): 1:

1.

4. The method for preparing a Dendrobium officinale extract according to claim 1, wherein The pH of the PBS buffer in S2 is 5-6.

5. The method for preparing a Dendrobium officinale extract according to claim 1, wherein The number of viable bacteria in the activated bacterial solution of Lactobacillus rhamnosus in S3 is ≥10 9 CFU / mL.

6. The method for preparing a Dendrobium officinale extract according to claim 1, wherein The centrifugal speed in S3 is 8000-9000 rpm, and the centrifugal time is 10-20 min.

7. The method for preparing a Dendrobium officinale extract according to claim 1, wherein: The ultrasonic frequency in S4 is 10-20 kHz, and the power density is 0.3-0.5 W / cm 2 .

8. The method for preparing a Dendrobium officinale extract according to claim 1, wherein: The fermentation supernatant C and the water extract D in S5 are mixed in a volume ratio of (2-4):

1.

9. A Dendrobium officinale extract, obtained by the preparation method according to any one of claims 1 to 8.

10. A use of the Dendrobium officinale extract according to claim 9, characterized in that: The Dendrobium officinale extract can be applied to the field of intestinal anti-inflammatory.

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