Preparation method of visible component microscopic examination sample, detection device and electronic equipment

Abstract

The preparation method of the visible component microscopic examination sample comprises the following steps: preparing at least two samples with different light transmittances, and selecting at least two different sample treatment methods; comprising any two treatment methods as follows: a method A: for a light-proof to-be-detected sample, taking a set volume of light-proof sample, adding a set volume of water into the light-proof sample, and diluting to obtain an intermediate sample; taking the intermediate sample, and adding a reagent with a set volume or a set weight to obtain a microscopic examination sample; the method B comprises the following steps: for a to-be-detected semi-transparent sample, taking a semi-transparent sample with a set volume, and adding a reagent with a set volume or a set weight to obtain a microscopic examination sample; the method C comprises the following steps: enriching a light-transmitting sample with a set volume for a light-transmitting to-be-detected sample, and adding a reagent with a set volume or a set weight into an enriched intermediate sample with a set volume to obtain a microscopic sample. Corresponding methods are selected according to different light transmittances of the to-be-detected samples, so that the to-be-detected samples in different states can be prepared into microscopic examination samples suitable for efficient imaging of a microscope.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of tangible substance analysis based on microscopic magnified images, and particularly relates to a tangible substance microscopic examination sample preparation method, a detection device and an electronic equipment. BACKGROUND

[0002] In tangible substance detection, various types and states of samples to be detected are involved.

[0003] In order to efficiently operate the device, the microscopic examination sample needs to be adjusted to an optimal or most suitable state for imaging. How to quickly and efficiently adjust different samples to a state suitable for microscopic examination is a technical problem to be solved.

[0004] The applicant has proposed a series of Chinese patents, such as

[0005] 1. CN2020112669290, "Cell analysis method and system and quantitative method and system";

[0006] 2. CN2020112669182, "Cell suspension sample imaging method and system and kit";

[0007] 3. CN2022104799126, "Microscopic image acquisition device rapid focusing method and microscopic image acquisition method";

[0008] 4. CN2023110496905, "Blood urine feces (multi-parameter) tangible substance detection device, chip and method".

[0009] A brand-new technical solution is used to measure the content of tangible substances in blood, urine and feces. The basis for using the above method to detect the sample to be detected is that the content of tangible substances in the microscopic examination sample is in a relatively balanced state suitable for efficient imaging by a microscope.

[0010] The body fluids of the human body include blood, urine, body cavity effusion, and feces which can be diluted with water into a suspension. The body cavity effusion includes pleural effusion, peritoneal effusion, joint cavity effusion, pericardial fluid, ventricular hydrocephalus, cerebrospinal fluid, and testicular sheath cavity effusion. Different body fluids, different body cavity fluids from different parts, and different body cavity fluids from the same part due to different diseases or individual characteristics vary greatly in concentration and have different colors.

[0011] Measurement is based on images, and images are based on microscopic examination samples. Different states and characteristics of samples to be detected need to be treated differently. Before the process of making different samples into microscopic examination samples suitable for imaging and recognition by a machine, they are all treated differently, which is complicated, time-consuming, labor-intensive and inefficient.

[0012] How to efficiently identify different types of samples to be detected, prepare the corresponding method for the microscopic sample, and make the content of the tangible substance in the microscopic sample in a relatively balanced state suitable for high-efficiency imaging of the microscope, is a technical problem to be solved. SUMMARY

[0013] In this application, the inventor proposes a method for preparing a microscopic sample with formed components. According to the different light transmittances of the samples to be detected, the corresponding sample processing method is selected, so that different states of the samples to be detected can be prepared into microscopic samples with formed components in a relatively balanced state suitable for high-efficiency imaging of the microscope.

[0014] The technical solution of the technical problem solved by the present application is a method for preparing a microscopic sample with formed components, which includes preparing at least two samples with different light transmittances and selecting at least two different sample processing methods. The method includes any two of the following processing methods: method A: for non-transparent samples to be detected, a certain volume of non-transparent samples is diluted with a certain volume of water to obtain an intermediate sample; the intermediate sample is taken and a certain volume or weight of reagent is added to obtain a microscopic sample; method B: for semi-transparent samples to be detected, a certain volume of semi-transparent samples is taken and a certain volume or weight of reagent is added to obtain a microscopic sample; method C: for transparent samples to be detected, a certain volume of transparent samples is enriched, a certain volume of the enriched intermediate sample is taken, and a certain volume or weight of reagent is added to obtain a microscopic sample.

[0015] The above-mentioned sample to be detected is irradiated by a light source, and the light transmittance of the sample to be detected is determined according to the transmission degree of the light emitted by the light source.

[0016] The above-mentioned method for preparing a microscopic sample with formed components includes any one of the following technical features: TA1: the light source is a display screen displaying a background or color block with a set brightness; TA2: the light source is a display screen displaying a text or symbol with a set brightness; TA3: the color of the background or color block displayed by the display screen with a set brightness is different from the color of the sample to be detected; TA4: the color of the text or symbol displayed by the display screen with a set brightness is different from the color of the sample to be detected.

[0017] The above-mentioned method for preparing a microscopic sample with formed components includes any one of the following technical features: TB1: the reagent is a dry powder reagent; TB2: the reagent includes new methylene blue.

[0018] The above-mentioned method for preparing a microscopic sample with formed components includes any one of the following technical features: TC1: the sample to be detected is blood; TC2: the sample to be detected is urine; TC3: the sample to be detected is feces; TC4: the sample to be detected is a body cavity fluid.

[0019] The above-mentioned method for preparing a microscopic sample of a visible component, in method A, for a non-transparent sample to be detected, a non-transparent sample of a set volume K11 is taken, physiological saline of a set volume K111 is added to obtain an intermediate sample; a volume K112 of the intermediate sample is taken, and a set volume or a set weight of dry powder reagent is added to obtain a microscopic sample.

[0020] In method B: for a semi-transparent sample to be detected, a semi-transparent sample of a set volume K12 is taken, and a set volume or a set weight of dry powder reagent is added to obtain a microscopic sample.

[0021] In method C: for a transparent sample to be detected, a transparent sample of a set volume K13 is taken, a set volume K131 of the enriched intermediate sample is taken, and a set volume or a set weight of dry powder reagent is added to obtain a microscopic sample.

[0022] The technical solution for solving the technical problem of the present application can also be a visible component detection device, which comprises a light source for irradiating a sample to be detected, and distinguishing the light transmission state of the sample to be detected.

[0023] The above-mentioned visible component detection device comprises any one of the following technical features: TD1: the light source is a display screen displaying a background or a color block of a set brightness; TD2: the light source is a display screen displaying a character or a symbol of a set brightness; TD5: the light source is a display screen displaying a background or a color block of a set brightness; the color of the background or the color block is different from the color of the sample to be detected; TD6: the light source is a display screen displaying a character or a symbol of a set brightness; the color of the character or the symbol is different from the color of the sample to be detected; TD7: the light source is an independent light source; TD8: the light source is an independent light source; the color of the light source is different from the color of the sample to be detected.

[0024] The technical solution for solving the technical problem of the present application can also be an electronic device, which comprises a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the above-mentioned program to realize the above-mentioned method for preparing a microscopic sample of a visible component according to any one of claim 3.

[0025] One of the technical effects of the above-mentioned technical solution is that according to the different light transmission of the sample to be detected, a corresponding sample processing method is selected, so that the sample to be detected in different states can be prepared into a microscopic sample with relatively balanced visible components suitable for efficient imaging by a microscope, and the detection efficiency is improved.

[0026] One of the technical effects of the above-mentioned technical solution is that through the judgment of the light transmission of the liquid by the human eye, the liquid to be detected is divided into two or more concentration interval ranges, which can cope with most different sample concentration intervals, and reduces the difficulty of preparing a microscopic sample.

[0027] One of the technical effects of the above technical solution is that by judging the light transmittance of the liquid with the human eye, different colors of light or different colors of background can effectively distinguish the concentration of samples of different colors, greatly reducing the difficulty of preparing the sample for microscopic examination.

[0028] One of the technical effects of the above technical solution is that using a display screen as a background light source or a background pattern can not only accurately control the brightness of the background, but also control the color of the background. In the modern society where displays are widely distributed, the use cost of standard colorimetric or turbidimetric cards is reduced, and there is no concern about the loss or color aging of the colorimetric or turbidimetric cards.

[0029] One of the technical effects of the above technical solution is that using a display screen to display patterns or text makes it easy for operators to operate, and almost no learning is required to distinguish the concentration range of the sample.

[0030] One of the technical effects of the above technical solution is that the light source irradiates the sample to be detected, and the light transmittance of the sample to be detected is judged according to the degree of transmission of the light emitted by the light source. The light irradiation method for detecting the transmission degree of the sample to be detected is more objective and quantifiable.

[0031] One of the technical effects of the above technical solution is that the light source is a display screen that displays a background or color block with a set brightness, or the light source is text or symbols with a set brightness, which is easier to recognize and easier to implement.

[0032] One of the technical effects of the above technical solution is that the reagent is a dry powder reagent, which is convenient to add, the sample preparation is easier, the amount of liquid reagent is reduced, and the efficiency is higher.

[0033] One of the technical effects of the above technical solution is that the sample preparation method is compatible with blood, urine, feces, and body cavity fluid.

[0034] One of the technical effects of the above technical solution is that for non-transparent samples to be detected, a corresponding volume of the sample is prepared with a corresponding volume of physiological saline, and then a corresponding volume of the intermediate sample is added to a corresponding amount of dry powder reagent to set the concentration of the formed components of the sample for microscopic examination to a certain range, thereby improving the preparation efficiency. There is no need to calculate the preparation ratio temporarily.

[0035] One of the technical effects of the above technical solution is that for semi-transparent samples to be detected, a set volume K12 of the semi-transparent sample is added to a set volume or set weight of dry powder reagent to obtain a sample for microscopic examination. Through the cooperation of different light transmittances and different amounts of dry powder reagent, the preparation efficiency is high.

[0036] One of the technical effects of the above technical solution is that for the light-transmitting sample to be detected, a set volume K13 of light-transmitting sample is enriched, a set volume K131 of the enriched intermediate sample is taken, and a set volume or set weight of dry powder reagent is added to obtain a microscopic sample. By enrichment, the content of formed elements in the microscopic sample is adjusted to a range suitable for imaging observation and analysis, improving the efficiency of preparation.

[0037] One of the technical effects of the above technical solution is that the formed element detection device provides a light source for sample preparation, eliminating the need for a specific light source during sample preparation, resulting in higher system efficiency. The provision of a uniform standard light source allows for more accurate judgment.

[0038] One of the technical effects of the above technical solution is that the formed element detection device provides a light source for sample preparation, eliminating the need for a specific light source during sample preparation, resulting in higher system efficiency. The provision of a uniform standard light source allows for more accurate judgment. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figures 1 to 9 is a flowchart of a method for preparing a formed element microscopic sample Figures 1 to 9 ;

[0040] Figure 10 is a schematic diagram of a formed element microscopic sample Figure 1 ;

[0041] Figure 11 is a schematic diagram of a formed element microscopic sample Figure 2 . DETAILED DESCRIPTION

[0042] The content of the present application will be further described in detail below in conjunction with the drawings. It should be noted that the following is a description of the preferred embodiments of the present application and does not constitute any limitation on the present application. The description of the preferred embodiments of the present application is only as an illustration of the general principles of the present application. The numbers "first", "second" and "A", "B" in the present application are only for convenience of explanation and do not represent the order of time or space. The combination of letters and numbers "TA", "TB", "H" in the present application is only for convenience of explanation, and the specific meaning is determined by the specific words referred to.

[0043] As Figures 1 to 4An embodiment of the method for preparing a microscopic sample with components includes preparing at least two samples with different light transmittances, and selecting at least two different sample processing methods. The processing methods include any two of the following: Method A: for a non-transparent sample to be detected, a set volume of the non-transparent sample is taken and diluted with a set volume of water to obtain an intermediate sample; the intermediate sample is taken and a set volume or a set weight of a reagent is added to obtain a microscopic sample; Method B: for a semi-transparent sample to be detected, a set volume of the semi-transparent sample is taken and a set volume or a set weight of a reagent is added to obtain a microscopic sample; Method C: for a transparent sample to be detected, a set volume of the transparent sample is enriched, a set volume of the enriched intermediate sample is taken, and a set volume or a set weight of a reagent is added to obtain a microscopic sample.

[0044] Non-transparent: under normal indoor lighting conditions, the sample cannot be seen through, and other objects cannot be seen through the sample; the sample thickness is 5-10 mm. Transparent: under normal indoor lighting conditions, the sample can be seen through, and a clear image of other objects can be seen through the sample; the sample thickness is 5-10 mm. Semi-transparent: between transparent and non-transparent, under normal indoor lighting conditions, the sample can be seen through, and a blurred image of other objects can be seen through the sample; the sample thickness is 5-10 mm.

[0045] The sample to be detected is any one or more of blood, urine, feces, and body cavity fluid.

[0046] For example, Figure 5 In an embodiment of the method for preparing a microscopic sample with components, the light source irradiates the sample to be detected, and the light transmittance of the sample to be detected is determined according to the degree of transmission of the light emitted by the light source.

[0047] For example, Figure 6 In an embodiment of the method for preparing a microscopic sample with components, the light source is a display screen displaying a background or a color block with a set brightness.

[0048] For example, Figure 7 In an embodiment of the method for preparing a microscopic sample with components, the light source is a display screen displaying a background or a color block with a set brightness.

[0049] For example, Figure 8 In an embodiment of the method for preparing a microscopic sample with components, in Method A, for a non-transparent sample to be detected, a set volume K11 of the non-transparent sample is taken, and a set volume K111 of physiological saline is added to obtain an intermediate sample; a volume K112 of the intermediate sample is taken, and a set volume or a set weight of a dry powder reagent is added to obtain a microscopic sample. The specific quantities of the set volume K11, the set volume K111, and the volume K112 can be set according to different samples. Specifically, in Figure 8In the embodiment of the preparation method of the microscopic sample with components, in method B, for the semi-transparent sample to be detected, a semi-transparent sample with a set volume K12 is taken, and a dry powder reagent with a set volume or a set weight is added to obtain the microscopic sample. The set volume K12 and the amount of the dry powder reagent with a set volume or a set weight added can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample,

[0050] As Figure 8 In the embodiment of the preparation method of the microscopic sample with components, in method B, for the semi-transparent sample to be detected, a semi-transparent sample with a set volume K12 is taken, and a dry powder reagent with a set volume or a set weight is added to obtain the microscopic sample. The set volume K12 and the amount of the dry powder reagent with a set volume or a set weight added can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample, Figure 8 In the embodiment of the preparation method of the microscopic sample with components, in method B, for the semi-transparent sample to be detected, a semi-transparent sample with a set volume K12 is taken, and a dry powder reagent with a set volume or a set weight is added to obtain the microscopic sample. The set volume K12 and the amount of the dry powder reagent with a set volume or a set weight added can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample,

[0051] As Figure 8 In the embodiment of the preparation method of the microscopic sample with components, in method C, for the transparent sample to be detected, a transparent sample with a set volume K13 is taken, an enriched sample with a set volume K131 is taken after enrichment, and a dry powder reagent with a set volume or a set weight is added to obtain the microscopic sample. The specific amount of the set volume K13 and the set volume K131 can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample, Figure 8 In the embodiment of the preparation method of the microscopic sample with components, in method C, for the transparent sample to be detected, a transparent sample with a set volume K13 is taken, an enriched sample with a set volume K131 is taken after enrichment, and a dry powder reagent with a set volume or a set weight is added to obtain the microscopic sample. The specific amount of the set volume K13 and the set volume K131 can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample,

[0052] As Figure 9 In the embodiment of the preparation method of the microscopic sample with components, in method A, for the opaque sample to be detected, an opaque sample with a set volume K21 is taken, and a liquid reagent with a set volume T21 is added to obtain the microscopic sample. The specific amount of the set volume K21 and the set volume T21 can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample, Figure 9 In the embodiment of the preparation method of the microscopic sample with components, in method A, for the opaque sample to be detected, an opaque sample with a set volume K21 is taken, and a liquid reagent with a set volume T21 is added to obtain the microscopic sample. The specific amount of the set volume K21 and the set volume T21 can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample,

[0053] As Figure 9 In the embodiment of the preparation method of the microscopic sample with components, in method B, for the semi-transparent sample to be detected, a semi-transparent sample with a set volume K22 is taken, and a liquid reagent with a set volume T22 is added to obtain the microscopic sample. The set volume K22 and the amount of the liquid reagent with a set volume T22 added can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample, Figure 9 In the embodiment of the preparation method of the microscopic sample with components, in method B, for the semi-transparent sample to be detected, a semi-transparent sample with a set volume K22 is taken, and a liquid reagent with a set volume T22 is added to obtain the microscopic sample. The set volume K22 and the amount of the liquid reagent with a set volume T22 added can be set according to different samples. Specifically, in the embodiment of the preparation method of the body cavity fluid sample,

[0054] As Figure 9In some embodiments of the method for preparing a sample for microscopic examination, in method C, for a sample to be examined, a set volume K23 of the sample is taken, a set volume K231 of the sample after enrichment is taken, and a set volume T23 of the liquid reagent is added to obtain a sample for microscopic examination. The specific volume of K23, K231 and T23 can be set according to different samples. Specifically, in Figure 9 some embodiments of the method for preparing a sample of body cavity fluid, K23 = 3-5 ml, K231 = 150 μl, and T23 = 2500 μl.

[0055] In some embodiments not shown in the drawings, the reagent described above includes new methylene blue.

[0056] In some embodiments of the device for detecting a formed element not shown in the drawings, a light source is provided for illuminating the sample to be examined to distinguish the light transmission state of the sample to be examined. The light source can be an independent light source, or a non-independent light source such as a light source illuminated by a screen. The light source can be a background or color block of a display screen with a set brightness. Alternatively, the light source can be a text or symbol displayed on a display screen with a set brightness.

[0057] In some embodiments of the device for detecting a formed element, the light source is a text or symbol displayed on a display screen with a set brightness. As shown in Figure 10 , three different samples are shown from left to right, which are a non-transparent sample, a semi-transparent sample, and a transparent sample. The transparent sample can clearly see the text displayed on the screen. The semi-transparent sample cannot clearly see the text displayed on the screen, but can see the blurred text. The non-transparent sample cannot see the text displayed on the screen at all.

[0058] As shown in Figure 11 , another three different samples are shown from left to right, which are a non-transparent sample, a semi-transparent sample, and a transparent sample. Figure 11 The non-transparent sample and the semi-transparent sample in are colored samples, and in this case, the color of the background or color block of the display screen with a set brightness should be different from the color of the sample to be examined to distinguish them.

[0059] In some corresponding embodiments, the light source is a background or color block of a display screen with a set brightness; the color of the background or color block is different from the color of the sample to be examined.

[0060] In some other corresponding embodiments, the light source is a text or symbol displayed on a display screen with a set brightness; the color of the text or symbol is different from the color of the sample to be examined.

[0061] In some other corresponding embodiments, the light source is an independent light source; the color of the light source is different from the color of the sample to be examined.

[0062] An electronic device comprising a memory, a processor, and a computer program stored on the memory and executable on the processor, said processor implementing the above-mentioned method for preparing a sample for microscopic examination when executing said program.

[0063] The application has been described and illustrated according to a preferred embodiment and several alternatives, but the application is not to be limited by the specific description in this specification. Other alternative or equivalent components can also be used to practice the application.

Claims

1. A method for preparing a sample for morphological analysis, characterized in that... : This includes preparing samples with at least two different transmittances. Choose at least two different sample processing methods; including any two of the following: Method A: For opaque samples to be tested, take a set volume of opaque sample and dilute it with a set volume of water to obtain an intermediate sample; take the intermediate sample and add a set volume or weight of reagent to obtain a microscopic sample. Method B: For a semi-transparent sample to be tested, take a set volume of the semi-transparent sample, add a set volume or set weight of reagent, and obtain a microscopic sample. Method C: For the light-transmitting sample to be tested, take a set volume of light-transmitting sample for enrichment, take an intermediate sample after enrichment of a set volume, add a set volume or set weight of reagent, and obtain a microscopic sample.

2. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, The light transmittance of the sample to be tested is determined by illuminating the sample with a light source and measuring the degree to which the sample transmits the light emitted by the light source.

3. The method for preparing formed sample for microscopic examination according to claim 2, characterized in that, Includes any one of the following technical features: TA1: The light source is the background or color block with a set brightness for the display screen; TA2: The light source is a display screen that shows text or symbols with a set brightness; TA3: The light source is a display screen with a set brightness, and the color of the background or color block is different from the color of the sample to be tested; TA4: The light source is a display screen where the color of the text or symbol with a set brightness is different from the color of the sample to be tested.

4. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, Includes any one of the following technical features: TB1: The reagent is a dry powder reagent; TB2: The reagent includes neomethylene blue.

5. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, Includes any one of the following technical features: TC1: The sample to be tested is blood; TC2: The sample to be tested is urine; TC3: The sample to be tested is feces; TC4: The sample to be tested is body cavity fluid.

6. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, In Method A, for an opaque sample to be tested, take an opaque sample of a set volume K11 and add a set volume K111 of physiological saline to obtain an intermediate sample; take an intermediate sample of volume K112 and add a set volume or set weight of dry powder reagent to obtain a microscopic sample.

7. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, In Method B: For a semi-transparent sample to be tested, take a semi-transparent sample of a set volume K12, add a set volume or set weight of dry powder reagent, and obtain a microscopic sample.

8. The method for preparing formed sample for microscopic examination according to claim 1, characterized in that, In Method C: For the light-transmitting sample to be tested, a set volume K13 of the light-transmitting sample is enriched, and a set volume K131 of the enriched intermediate sample is added to a set volume or a set weight of dry powder reagent to obtain a microscopic examination sample.

9. A formed element detection device, characterized in that, It includes a light source, which is used to illuminate the sample to be tested and distinguish the light transmittance of the sample.

10. The formed element detection device according to claim 1, characterized in that, Includes any one of the following technical features: TD1: The light source is the background or color block with a set brightness displayed on the screen; TD2: The light source is a display screen that shows text or symbols with a set brightness; TD5: The light source is a background or color block with a set brightness displayed on the screen; the color of the background or color block is different from the color of the sample to be tested; TD6: The light source is a display screen showing text or symbols with a set brightness; the color of the text or symbols is different from the color of the sample to be tested; TD7: The light source is an independent light source; TD8: The light source is an independent light source; the color of the light source is different from the color of the sample to be tested.

11. An electronic device, characterized in that, This includes memory, processor, and computer programs stored in memory and capable of running on the processor. When the processor executes the program, it implements the method for preparing formed sample microscopy specimens as described in any of claims 3.

Images