Method for allele matching of donor mesenchymal stem cells and receptors

By isolating cells from donor perinatal tissues for HLA genotyping and allele matching, and combining this with immunostimulatory factors to simulate the in vivo environment, the risk of immune rejection caused by HLA mismatch in existing technologies has been resolved. This has enabled highly efficient in vivo survival and implantation of MSCs, improving the safety and precision of regenerative medicine and cell therapy.

CN121999862APending Publication Date: 2026-05-08ZHEJIANG TIANYUAN BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511808370.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-03
Publication Date
2026-05-08

AI Technical Summary

Technical Problem

The lack of standardized methods for donor and recipient mesenchymal stem cell allele matching in existing technologies makes it impossible to predict the risk of immune rejection caused by HLA mismatch, affecting the in vivo survival and implantation efficiency of MSCs.

Method used

This invention provides a method for allelic matching between donor mesenchymal stem cells and recipients. Cells are isolated from the donor's perinatal tissue, HLA genotyping is performed, and the results are compared with the recipient to establish a standardized matching database. Immunostimulatory factors are used to simulate the in vivo environment to induce HLA antigen expression, and the matching degree is quantitatively assessed to construct an efficient cell bank.

Benefits of technology

This approach enables effective prediction of HLA mismatch immune rejection risk before treatment, improves in vivo survival and implantation efficiency of MSCs, ensures the reliability and safety of assessment results, optimizes resource allocation and assessment efficiency, and enhances the safety and precision of allogeneic mesenchymal stem cell therapy.

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Abstract

The invention provides a method for allele matching of donor mesenchymal stem cells and a receptor. The method comprises the following steps: S10, separating mesenchymal stem cells from perinatal tissue of a donor; s20, extracting genome DNA (deoxyribonucleic acid) of the mesenchymal stem cells, and performing HLA (human leukocyte antigen) genetic typing; s30, performing HLA (human leukocyte antigen) genetic typing on the DNA of the receptor; s40, performing allele spectrum comparison on the HLA gene of the receptor and the HLA gene of the mesenchymal stem cell, and calculating the matching degree; s50, judging the matching degree of the receptor and the mesenchymal stem cells according to the matching degree; and S60, storing the HLA genotyping result of the mesenchymal stem cells and the matching degree in a database, and establishing a matching library for matching the donor mesenchymal stem cells and the receptor. According to the method, a set of standardized system is established, compatibility evaluation of a donor and a receptor at the allele level is achieved, the immunological rejection risk caused by HLA mismatching is effectively predicted and avoided before treatment, and the in-vivo survival and implantation efficiency of MSCs is improved.
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