A composition for improving osteoarthritic inflammation and increasing bone density and use thereof

Abstract

The application belongs to the technical field of health food, and provides a composition for improving osteoarthritis and increasing bone density and application thereof.The composition comprises, in terms of weight fraction, 1-600 parts of eggshell membrane, 1-80 parts of non-denatured type II collagen, 1-900 parts of mangosteen fruit powder and 1-1000 parts of sophora fruit powder.The composition of the application has reasonable compatibility, and each component has clear efficacy, and has remarkable effect in improving bone and joint damage, such as improving osteoarthritis and increasing bone density.

Description

Technical Field

[0001] This invention belongs to the field of health food technology, and provides a composition for improving osteoarthritis and increasing bone density, and its application. Background Technology

[0002] Osteoarthritis is a common joint disease, mainly characterized by joint pain, swelling, and impaired function. During the inflammatory process of arthritis, inflammatory mediators activate osteoclasts, leading to increased bone resorption, while simultaneously inhibiting osteoblast activity, thus causing a decrease in bone density. Furthermore, arthritis patients may experience reduced bone load due to pain or limited mobility, further exacerbating the decline in bone density. Clinically, nonsteroidal anti-inflammatory drugs (NSAIDs) and corticosteroids are commonly used for treatment, but these drugs may have negative effects on bones. Meanwhile, food-grade ingredients, with their green and safe properties, are receiving increasing attention in the field of osteoarthritis management.

[0003] Eggshell membrane (ESM), located between the egg white and the eggshell, is a complex protein mainly composed of corneal tissue and mucopolysaccharides. When dissolved, it yields a mixture of soluble macromolecular compounds such as acetylglucosamine, galactosylsulfonate, chondroitin, and amino acids. Numerous studies have reported the antioxidant, anti-inflammatory, joint health-promoting, antibacterial, skin-beautifying, and other health-improving bioactivities of fresh eggshell membrane and its derivatives.

[0004] For example, Chinese invention patent application CN120021779A discloses a composition for improving osteoarthritis, comprising the following components: type II collagen chondroitin powder, eggshell membrane, and N-acetylglucosamine. In a specific ratio, the type II collagen chondroitin powder, eggshell membrane, and N-acetylglucosamine can effectively relieve joint swelling and reduce inflammation in rats with arthritis; at the same time, it can also increase glycosaminoglycans in synovial tissue, which has a good effect on promoting wound healing.

[0005] For example, Chinese invention patent application CN109480213A discloses a sports nutrition product combination composition with joint maintenance and repair, mainly comprising two parts: Partner One and Partner Two. Partner One's main ingredients include eggshell membrane powder, cartilage collagen peptides, hyaluronic acid, calcium salts, magnesium salts, vitamin C, vitamin D, and vitamin K. Partner Two's main ingredients include pomegranate peel extract, mangosteen extract, dimethyl sulfone, frankincense extract, and curcumin. The combination of these two formulas can provide comprehensive protection for joints and can provide targeted care based on the degree of cartilage damage and joint pain observed in athletes.

[0006] However, most existing food-derived medicinal compositions for improving osteoarthritis focus only on inflammation relief and cartilage protection, failing to simultaneously address bone density loss. Long-term use is insufficient to improve bone loss caused by joint damage, resulting in limited overall improvement. Furthermore, the high cross-linking and poor solubility of eggshell membranes lead to low bioavailability. Many studies have applied processes such as pulverization, hydrolysis, and fermentation to prepare various eggshell membrane derivatives, including eggshell membrane micropowders and peptides, to better develop the bioactivity and applications of eggshell membranes.

[0007] Based on this, by rationally combining raw materials that are both food and medicine or improving extraction processes, the active components can be combined synergistically. This can not only inhibit joint inflammation and relieve swelling and pain symptoms, but also regulate the activity of osteoclasts and osteoblasts to improve the problem of decreased bone density. At the same time, it can also avoid the adverse reactions of clinical drugs and is safe for long-term use. Summary of the Invention

[0008] To address the problems existing in the prior art, this invention provides a composition for improving osteoarthritis and increasing bone density, and its application. The components in the composition of this invention are rationally combined, resulting in significant therapeutic effects in improving osteoarthritis, protecting cartilage, and increasing bone density.

[0009] The technical solution of the present invention is as follows: In a first aspect, the present invention provides a composition for improving bone and joint injuries, the composition comprising, by weight parts: 1-600 parts eggshell membrane, 1-80 parts non-denatured type II collagen, 1-900 parts mangosteen fruit powder and 1-1000 parts sophora japonica flower powder.

[0010] Preferably, the composition comprises, by weight, 1-300 parts eggshell membrane, 1-40 parts non-denatured type II collagen, 1-600 parts mangosteen fruit powder, and 1-500 parts sophora japonica flower powder.

[0011] More preferably, the composition comprises, by weight, 10-20 parts eggshell membrane, 2-3 parts non-denatured type II collagen, 20-40 parts mangosteen fruit powder, and 25-45 parts sophora japonica flower powder.

[0012] More preferably, the composition comprises, by weight, 15 parts eggshell membrane, 1.5 parts non-denatured type II collagen, 30 parts mangosteen fruit powder, and 35 parts sophora japonica flower powder.

[0013] Unless otherwise specified, the eggshell membrane of the present invention can be an untreated eggshell membrane, or an eggshell membrane extract prepared by a certain extraction and purification process; preferably, it is an eggshell membrane extract.

[0014] More preferably, the eggshell membrane is an eggshell membrane extract, and the preparation process of the eggshell membrane extract is as follows: (1) Eggshell membrane pretreatment: After crushing the eggshell membrane, add it to water, heat and stir to obtain solution 1; (2) Extraction of chondroitin sulfate: Chlorate and sodium hydroxide were added to solution 1 for extraction. After extraction, alcohol precipitation was performed to obtain chondroitin sulfate extract. (3) Preparation of small molecule active peptides: Add a compound enzyme to the extract for enzymatic hydrolysis, and then inactivate the enzyme to obtain eggshell membrane hydrolysate. Filter, concentrate and dry to obtain small molecule active peptides. (4) The chondroitin sulfate extract is combined with small molecule active peptides to obtain the final product; The complex enzyme consists of aminochondroitin, papain, and trypsin.

[0015] The mass ratio of aminochondroitin extractase, papain and trypsin is 1:0.05-0.1:0.2-0.4.

[0016] In step (1), the temperature for heating and stirring is 70-80℃; the heating and stirring time is 1-2h.

[0017] In step (2), the concentration of chlorate in solution 1 is 15-25 mg / mL; the concentration of sodium hydroxide in solution 1 is 2-4 mg / mL; the extraction temperature is 70-80℃; and the extraction time is 20-40 min.

[0018] In step (3), the temperature for enzymatic hydrolysis is 45-55℃ and the time for enzymatic hydrolysis is 1-3h.

[0019] In a second aspect, the present invention provides the use of the composition described in any of the above claims in products for improving osteoarthritis.

[0020] Furthermore, the improvement of bone and joint damage includes: improving osteoarthritis and / or increasing bone density.

[0021] Furthermore, the product can be food.

[0022] Furthermore, the dosage form of the food is any formulation suitable for oral administration, including but not limited to any one of liquid dosage forms, jelly dosage forms, gel dosage forms, tablets, powders, and capsules. The food also contains excipients and / or additives acceptable in the food industry.

[0023] As some preferred embodiments of the present invention, the excipients include, but are not limited to, one or more of maltodextrin, starch, modified starch, yeast products, natural sugar, processed sugar, dietary fiber, fruit and vegetable powder, and plant and animal extracts.

[0024] As some preferred embodiments of the present invention, the additives include, but are not limited to, one or more of flavor enhancers, sweeteners, emulsifiers, flavorings, stabilizers, thickeners, antioxidants, acidity regulators, preservatives, and colorants.

[0025] Thirdly, the present invention also provides the use of the composition in products that improve osteoarthritis and increase bone density.

[0026] Furthermore, the product is a health food, and the dosage form of the health food is any formulation suitable for oral administration, including but not limited to any one of liquid dosage form, jelly dosage form, gel dosage form, tablet, powder and capsule dosage form.

[0027] Furthermore, the health food also contains excipients and / or additives acceptable in the food industry.

[0028] As some preferred embodiments of the present invention, the excipients include, but are not limited to, one or more of maltodextrin, starch, modified starch, yeast products, natural sugar, processed sugar, dietary fiber, fruit and vegetable powder, and plant and animal extracts.

[0029] As some preferred embodiments of the present invention, the additives include, but are not limited to, one or more of flavor enhancers, sweeteners, emulsifiers, flavorings, stabilizers, thickeners, antioxidants, acidity regulators, preservatives, and colorants.

[0030] Compared with the prior art, the present invention has the following beneficial effects: (1) The eggshell membrane in the composition of the present invention is rich in chondroitin sulfate, collagen and hydrolyzed active peptides, which directly provide structural raw materials for cartilage. Non-denatured type II collagen plays a role in protecting cartilage by regulating immunity. Mangosteen fruit powder and Sophora japonica flower powder synergistically inhibit the NF-κB pathway and reduce the production of joint inflammatory mediators. At the same time, Sophora japonica flower powder can also stimulate osteoblast activity and achieve bone tissue repair. The mechanism of action of each component is clear, and it has significant effects on improving osteoarthritis, repairing bone tissue and increasing bone density. (2) In this invention, chondroitin sulfate is separated by alkaline salt extraction of eggshell membrane, and the remaining protein is hydrolyzed to obtain small molecule active peptides, which improves the original high cross-linking and poor solubility that leads to slow absorption rate, thereby improving bioavailability. Attached Figure Description

[0031] Figure 1 Results of knee joint width changes in rats in each group; Figure 2 Results of the levels of IL-1β, IL-6 and TNF-α in the joint lavage fluid of each rat; Figure 3The results are for whole-body bone mineral density, femoral bone mineral density, and bone calcium content of each rat. Figure 1-3 In the middle, compared with the normal group, # P <0.05, ## P <0.01; compared with the model control group, & P <0.05, && P <0.01; compared with Example 3, ^ P <0.05, ^^ P <0.01. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] The raw materials used in the following comparative examples are from the following sources: Homemade eggshell membrane 1: Take eggshell membrane fragments and pass them through an 80-mesh sieve. Add them to water at a material-to-liquid ratio of 1:8. Heat the mixture to 75℃ and stir for 1 hour. Add chlorate (to a concentration of 20 mg / mL) and sodium hydroxide (to a concentration of 3 mg / mL). Incubate for 30 minutes. Add anhydrous ethanol to a solution concentration of 45 wt%. After precipitation, filter and collect the supernatant. Add 5% of the eggshell membrane mass of a complex enzyme (amino chondroitin, papain, and trypsin in a mass ratio of 1:0.1:0.3) to the supernatant. Enzymatically hydrolyze the mixture at 50℃ for 2 hours. After hydrolysis, inactivate the enzymes. Filter to remove unreacted enzymes and large protein molecules. Concentrate and dry to obtain the final product.

[0034] Homemade eggshell membrane 2: The only difference between this product and the self-made eggshell membrane 1 is the composition of the complex enzymes used in its preparation process. Specifically, it consists of aminochondroitin extract, papain, and trypsin in a mass ratio of 1.2:0.05:0.15.

[0035] Commercially available eggshell membranes were purchased from Shanghai Chengyou Industrial Co., Ltd. (model: MM); non-denatured type II collagen was purchased from Guangzhou Xiangpin Biotechnology Co., Ltd.; mangosteen fruit powder was purchased from Guangzhou Hanzhong Trading Co., Ltd.; sophora japonica flower powder was purchased from Beijing Xinda Hezhong International Trading Co., Ltd.; turmeric powder was purchased from Shaanxi Sinote Biotechnology Co., Ltd.; and hydrolyzed type II collagen was purchased from Shandong Baolijia Biotechnology Co., Ltd.

[0036] Example 1 A composition for improving bone and joint injuries comprises: 1.5 parts by weight of self-made eggshell membrane, 1 part by weight of non-denatured type II collagen, 100 parts by weight of mangosteen fruit powder, and 90 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0037] Example 2 A composition for improving bone and joint injuries comprises: 10 parts by weight of self-made eggshell membrane, 3 parts by weight of non-denatured type II collagen, 20 parts by weight of mangosteen fruit powder, and 45 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0038] Example 3 A composition for improving bone and joint injuries comprises: 1.15 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, 30 parts by weight of mangosteen fruit powder, and 35 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0039] Example 4 A composition for improving bone and joint injuries comprises: 120 parts by weight of self-made eggshell membrane, 3 parts by weight of non-denatured type II collagen, 20 parts by weight of mangosteen fruit powder, and 25 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0040] Example 5 A composition for improving bone and joint injuries comprises: 130 parts by weight of self-made eggshell membrane, 4 parts by weight of non-denatured type II collagen, 10 parts by weight of mangosteen fruit powder, and 15 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0041] Example 6 A composition for improving bone and joint injuries comprises: 15 parts by weight of commercially available eggshell membrane (MM), 1.5 parts by weight of non-denatured type II collagen, 30 parts by weight of mangosteen fruit powder, and 35 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the parts by weight and mixed thoroughly to obtain the composition.

[0042] Comparative Example 1 A composition for improving bone and joint injuries comprises: 115 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, and 75 parts by weight of mangosteen fruit powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0043] Comparative Example 2 A composition for improving bone and joint injuries comprises: 115 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, and 75 parts by weight of Sophora japonica powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0044] Comparative Example 3 A composition for improving bone and joint injuries comprises: 1.15 parts by weight of self-made eggshell membrane, 1.5 parts by weight of hydrolyzed type II collagen, 30 parts by weight of mangosteen fruit powder, and 35 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0045] Comparative Example 4 A composition for improving bone and joint injuries comprises: 1.15 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, 30 parts by weight of turmeric powder, and 35 parts by weight of sophora japonica powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0046] Comparative Example 5 A composition for improving bone and joint injuries comprises: 1.15 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, 30 parts by weight of mangosteen fruit powder, and 35 parts by weight of turmeric powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0047] Comparative Example 6 A composition for improving bone and joint injuries comprises: 215 parts by weight of self-made eggshell membrane, 1.5 parts by weight of non-denatured type II collagen, 30 parts by weight of mangosteen fruit powder, and 35 parts by weight of sophora japonica flower powder; the above-mentioned raw materials are taken according to the weight parts and mixed thoroughly to obtain the composition.

[0048] Test Example 1: Pharmacological effects of the composition of the present invention on a rat model of osteoarthritis. 1. Experimental grouping: Wistar male rats (weight 280±10g) were randomly divided into normal group, model group, positive group, Example 1-6 groups and Comparative Example 1-6 groups after 7 days of acclimatization, with 10 rats in each group.

[0049] 2. Modeling: 10% papain and 0.03 mol / L L-cysteine ​​were mixed at a 1:1 ratio, and the resulting solution was allowed to stand for 30 minutes before use. On days 1, 4, and 7 of the experiment, 0.2 mL of the above mixed solution was injected into the knee joint cavity of rats in the model group, positive group, Examples 1-6, and Comparative Examples 1-6 to induce a rat osteoarthritis model. The normal group was injected with an equal volume of physiological saline.

[0050] 3. Drug administration: After modeling, drug administration began on day 8 of the experiment. The positive group was administered amino acid glucose hydrochloride (Aoteling, manufactured by Ausnutria Pharmaceuticals) by gavage at a dose of 90 mg / kg. The Example 1-6 groups and the Comparative Example 1-6 groups were administered the corresponding composition at a dose of 0.32 g / kg·d by gavage. The normal group and the model group were administered the same dose of deionized water by gavage. Each rat was administered the drug once daily for 4 weeks. The entire experiment (i.e., modeling + drug administration) lasted for 5 weeks.

[0051] 4. Characterization: (1) Observation of general condition of rats: Observe the mental state, hair and food intake of rats.

[0052] (2) Changes in knee joint width in rats: Joint width was measured before drug administration, at week 2 of drug administration, and at week 4 of drug administration. Each knee joint was tested three times, and the average of the six results was taken. The results are shown in […]. Figure 1 .

[0053] (3) Two hours after the last oral administration, the rats were anesthetized, and physiological saline was injected into both knee joint cavities for irrigation. The joint lavage fluid was centrifuged at 1500 r / min for 15 min at 4℃, and the supernatant was separated, aliquoted, and stored at -80℃ for later use. The contents of IL-1β, IL-6, and TNF-α in the lavage fluid were determined according to the ELISA kit instructions. The results are shown in the table below. Figure 2 .

[0054] Depend on Figure 1 It can be seen that before drug administration, compared with the normal group, the width of the left and right ankle joints of rats in all groups was significantly increased. P <0.01), indicating successful establishment of the osteoarthritis model; after 2 weeks of administration, the ankle joint width of rats in all example groups was significantly reduced, and there was a significant difference compared with the model group. After 4 weeks of administration, the ankle joint width of rats in all example groups approached that of the normal group, especially in Example 3, which showed no significant difference from the normal group; while comparative groups 1-6 still showed significant differences from the normal group after 4 weeks of administration, and had no effect on improving arthritis ( P <0.01).

[0055] Depend on Figure 2 It can be seen that, compared with the normal group, the levels of pro-inflammatory factors IL-1β, IL-6, and TNF-α in the model group rats were significantly increased.P <0.01), indicating successful establishment of the osteoarthritis model; compared with the model group, the inflammation levels in each embodiment group were significantly reduced ( P <0.01), although each comparative example also showed varying degrees of decrease, the degree of decrease was lower than that of each example group, especially compared with Example 3 group, all of which showed significant differences ( P <0.05 or P <0.01).

[0056] In summary, it can be seen that the eggshell membrane (especially the eggshell membrane obtained by enzymatic hydrolysis with a specific complex enzyme) in the composition of the present invention, combined with non-denatured type II collagen, mangosteen fruit powder and sophora japonica flower powder, has a significant effect on improving osteoarthritis inflammation. Among them, mangosteen fruit powder and sophora japonica flower powder play a significant synergistic anti-inflammatory role in the composition.

[0057] Test Example 2: Effects of the composition of the present invention on the skeleton of ovariectomized rats 1. Experimental grouping: SD female mice (weight 220±10g) were randomly divided into normal group, model group, Example 1-6 groups and Comparative Example 1-6 groups after 7 days of acclimatization, with 10 mice in each group.

[0058] 2. Modeling and Drug Administration: Except for the normal group, all rats in the other groups underwent bilateral ovariectomy. The normal group underwent the same surgical procedure, but the ovaries were not removed. Rats in the normal and model groups were given free access to water in addition to a normal diet every day. Rats in Examples 1-6 and Comparative Examples 1-6 were given free access to water in addition to a normal diet every day and were given 0.32g of the corresponding composition / kg·d for 90 days.

[0059] 3. Characterization: (1) Determination of bone mineral density in rats: After 90 days of feeding, the intervention ended. The rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL / kg), and their whole-body bone mineral density was tested. They were then sacrificed, and the femurs of both sides were dissected. After removing the soft tissue, the bone mineral density was measured. The average value of the bone mineral density of both femurs was taken as the result. The results are shown in the figure. Figure 3 .

[0060] (2) Determination of bone calcium content in rats: One femur of a rat was roasted to constant weight and then digested as a whole using a wet method. The bone calcium content was determined using an atomic absorption spectrophotometer. The results are shown below. Figure 3 .

[0061] Depend on Figure 3 It can be seen that, compared with the normal group, the model group rats had significantly lower whole-body bone mineral density, femoral bone mineral density, and bone calcium content. P<0.01), indicating that the rats developed osteoporosis symptoms after ovariectomy, and the model was successfully established. Compared with the model group, the whole body bone mineral density, femoral bone mineral density and bone calcium content of the rats in Examples 1-5 were significantly increased, and the osteoporosis symptoms were significantly improved. Although the whole body bone mineral density, femoral bone mineral density and bone calcium content of the rats in Example 6 and Comparative Examples 1-6 were increased, they were still far lower than those in Examples 1-5 and the normal group compared with the model group.

[0062] Combination Figure 1 and Figure 2 The results show that, compared with commercially available eggshell membranes, the eggshell membrane prepared by the present invention using a specific complex enzyme hydrolysis not only has a considerable effect on improving osteoarthritis, but also has a strong effect on improving osteoporosis, and has a more comprehensive effect on protecting bones.

[0063] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for improving osteoarthritis inflammation and increasing bone density, characterized in that, The composition comprises, by weight, 1-600 parts eggshell membrane, 1-80 parts non-denatured type II collagen, 1-900 parts mangosteen fruit powder, and 1-1000 parts sophora japonica flower powder.

2. The composition according to claim 1, characterized in that, The composition comprises, by weight, 1-300 parts eggshell membrane, 1-40 parts non-denatured type II collagen, 1-600 parts mangosteen fruit powder, and 1-500 parts sophora japonica flower powder.

3. The composition according to claim 2, characterized in that, The composition comprises, by weight, 10-20 parts eggshell membrane, 2-3 parts non-denatured type II collagen, 20-40 parts mangosteen fruit powder, and 25-45 parts sophora japonica flower powder.

4. The composition according to claim 3, characterized in that, The composition comprises, by weight, 15 parts eggshell membrane, 1.5 parts non-denatured type II collagen, 30 parts mangosteen fruit powder, and 35 parts sophora japonica flower powder.

5. The composition according to any one of claims 1-4, characterized in that, The eggshell membrane is an eggshell membrane extract, and the preparation process of the eggshell membrane extract is as follows: (1) Eggshell membrane pretreatment: After crushing the eggshell membrane, add it to water, heat and stir to obtain solution 1; (2) Extraction of chondroitin sulfate: Chlorate and sodium hydroxide were added to solution 1 for extraction. After extraction, alcohol precipitation was performed to obtain chondroitin sulfate extract. (3) Preparation of small molecule active peptides: Add a compound enzyme to the extract for enzymatic hydrolysis, and then inactivate the enzyme to obtain eggshell membrane hydrolysate. Filter, concentrate and dry to obtain small molecule active peptides. (4) The chondroitin sulfate extract is combined with small molecule active peptides to obtain the final product; The complex enzyme consists of aminochondroitin, papain, and trypsin.

6. The composition according to claim 5, characterized in that, The mass ratio of aminochondroitin extractase, papain, and trypsin is 1:0.05-0.1:0.2-0.

4.

7. The composition according to claim 5, characterized in that, In step (1), the temperature for heating and stirring is 70-80℃; the heating and stirring time is 1-2h.

8. The composition according to claim 5, characterized in that, In step (2), the concentration of chlorate in solution 1 is 15-25 mg / mL; the concentration of sodium hydroxide in solution 1 is 2-4 mg / mL.

9. The composition according to claim 5, characterized in that, In step (3), the temperature for enzymatic hydrolysis is 45-55℃; the time for enzymatic hydrolysis is 1-3h.

10. Use of the composition according to any one of claims 1-9 in a product for improving osteoarthritis and increasing bone density.

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