Sample multiple dilution device for quantitative detection

Abstract

The utility model relates to a sample multiple dilution device for quantitative detection. The problems that in the prior art, when samples with large dilution ratios are diluted, mutual aerosol pollution is prone to occurring between the samples, and confusion is prone to occurring between the samples with different dilution ratios are solved. The dilution device comprises a dilution device body and a rotary cover component, the dilution device body comprises a plate frame, a plurality of liquid containing wells are arranged on the plate frame, and liquid delivery channels used for being communicated with the liquid containing wells are arranged on the plate frame; the rotating cover component comprises a rotating cover body arranged in the liquid containing well, a rotating handle is arranged on the rotating cover body, and a liquid delivery opening capable of being communicated with the liquid delivery channel is formed in the side wall of the rotating cover body. The device has the advantages that the device is convenient to operate, cross contamination and confusion among different samples are effectively avoided, and the dilution accuracy is high.

Description

Technical Field

[0001] The utility model relates to the technical field of biological monitoring, in particular to a sample multiple dilution device for quantitative detection. Background Art

[0002] Cell and gene therapy, or CGT, is an emerging industry in the medical field that relies on the development of cell therapy and gene therapy technology. Through cell therapy and gene therapy for patients, cells and genes can be used as carriers to directly treat the root causes of patients' diseases, achieving treatment effects that are difficult to achieve with general clinical methods. As an emerging treatment method, CGT is an important driving force for leading the innovation of future medical methods. With the rapid development of biomedical treatment methods, the corresponding drug analysis methods must also keep pace with the times.

[0003] In view of the rapid development of the CGT field, various advanced detection technology platforms will be used for drug analysis and quality control in the cell and gene therapy industry. Including various safety-related process residue detection and virus titer detection. One example is: highly sensitive absolute quantitative droplet digital PCR is used for nucleic acid residue detection in viral vectors such as lentivirus, AAV, ADV, etc. The digital PCR detection sensitivity can reach 1cp / uL, but due to the narrow linear range of digital PCR detection, the general upper limit is 5000cp / uL. Therefore, these samples need to be diluted to the linear range, and these dilution multiples are generally greater than 1000. Another example is: high-sensitivity ELISA, such as single-molecule immunoassay, or chemiluminescence method is used for residual protein detection or virus physical detection. For example, in the process development of lentivirus, AVV, etc., the residual levels of upstream samples and most downstream BSA, HCP, etc. may be at the ug / mL or ng / mL level, but the linear detection level of high-sensitivity immunoassay is at ng / mL or pg / mL. This means that in order to obtain accurate detection, the sample needs to be diluted one thousand to tens of thousands times before it can be loaded for testing; in addition, when detecting the physical titer of lentivirus through P24 protein, the sample needs to be diluted more than tens of thousands times to obtain accurate results.

[0004] At present, in order to obtain accurate test results and reduce the uncertainty caused by dilution, the most effective dilution method is 10-fold dilution for the above-mentioned large dilution multiples; that is, mixing the sample and diluent in a volume ratio of 1:9 for 10-fold dilution. There are two traditional methods: one is to place a series of centrifuge tubes on a centrifuge tube rack, then add samples, cover the lids, mix, and then sample into the next gradient tube, repeat this operation many times, and finally obtain the final diluted sample. The disadvantage of this method is that when the amount of samples to be processed increases, it takes a lot of time, the operation is cumbersome, and it is easy to confuse samples, as well as confusion between different dilutions of the same sample, and mutual contamination between samples, especially when doing nucleic acid testing, it is more likely to cause mutual contamination; the other is to set parameters on the software through a large pipetting workstation, and then place the centrifuge tube on the centrifuge tube rack in the equipment cavity to complete the liquid addition operation. However, the disadvantage of this operation is that it will also cause mutual aerosol contamination between samples, which seriously affects the accuracy of the test results.

[0005] At present, for the dilution of samples with large dilution factors, it is urgent to develop new dilution devices or equipment to avoid aerosol contamination between samples during the sample dilution process, avoid confusion between samples or between different dilutions of the same sample, and ensure effective and accurate 10-fold dilution to achieve the above goals. Summary of the invention

[0006] The purpose of the utility model is to provide a sample multiple dilution device for quantitative detection in view of the above problems.

[0007] To achieve the above-mentioned purpose, the utility model adopts the following technical solutions: a sample multiple dilution device for quantitative detection, including a dilution device body and a rotating cover component, the dilution device body includes a plate rack, the plate rack is provided with a plurality of liquid wells, and the plate rack is provided with a liquid transfer channel for connecting the liquid wells; the rotating cover component includes a rotating cover body arranged in the liquid well, the rotating cover body is provided with a rotating handle, and the side wall of the rotating cover body is provided with a liquid transfer port that can be connected with the liquid transfer channel.

[0008] The main body of the dilution device is responsible for the liquid flow carrier of the entire sample dilution process through 4 liquid wells and 3 liquid transfer channels. Another important component of the main body of the dilution device is the rotating cover component, which acts as a "valve" in the entire sample dilution process, and controls the liquid flow of the sample dilution mixture in the liquid wells through the rotating cover valve to achieve a gradual 10-fold dilution.

[0009] In the above-mentioned sample dilution device for quantitative detection, the rotating cover body is cylindrical and hollow inside, the upper end of the rotating cover body is sealed and the lower end is completely open, and the rotating handle is arranged parallel to the opening of the liquid transfer port.

[0010] In the above-mentioned sample multiple dilution device for quantitative detection, a rotating cover direction indicator is marked on a side of the rotating handle facing the opening of the liquid transfer port.

[0011] In the above-mentioned sample dilution device for quantitative detection, the liquid transfer port is a circular port, and the opening diameter range of the liquid transfer port is 3.0mm-4.0mm, preferably 3.6mm, the inner diameter range of the rotating cover body is 9.0-9.2mm, preferably 9.1mm, and the outer diameter range is 0.65-9.67mm, preferably 9.66mm; the plate rack height range is 25mm-35mm, preferably 30mm; the plate rack thickness is 3mm-5mm, preferably 4mm.

[0012] In the above-mentioned sample multiple dilution device for quantitative detection, the liquid filling well includes an upper end and a lower end, the inner diameter of the upper end is larger than the inner diameter of the lower end, and the inner diameter of the upper end is 9.66 mm, and the inner diameter of the lower end is 9.1 mm; the height of the upper end is 8 mm, and the height of the lower end is 14 mm. The liquid filling well is a U-shaped well with a full opening at the upper end, and a circular opening is provided on a side of the upper end of the liquid filling well close to the liquid transfer channel, and the opening diameter is 3.0 mm-4.0 mm, preferably 3.6 mm.

[0013] In the above-mentioned sample multiple dilution device for quantitative detection, a 900uL quality control scale line is provided on the outside of the lower end of the liquid-filling well, and a hydrophilic treatment layer is provided on the circumferential inner wall of the liquid transfer channel.

[0014] In the above-mentioned sample multiple dilution device for quantitative detection, the bottom shape of the lower end of the liquid-filling well is V-shaped or U-shaped.

[0015] In the above-mentioned sample multiple dilution device for quantitative detection, a rotational positioning arrow of a rotary cover and digital markings for rotational positioning of the rotary cover in different dilution steps are provided on the upper end surface of the dilution device body and in the circumference of the liquid filling well.

[0016] In the above-mentioned sample multiple dilution device for quantitative detection, the upper end surface of the dilution device body and one side of the liquid filling well located at the head are respectively marked with A, B, and C sample column partition marks for indicating different channels, and the sides of the liquid filling well are marked with 10, 100, 1000, and 10000 dilution multiple marks for determining the dilution operation sequence from left to right.

[0017] In the above-mentioned sample multiple dilution device for quantitative detection, a sample identification area for filling in sample information with a marker pen is provided on the upper end surface of the dilution device body and on one side of the liquid well at the tail. The size of the sample identification area is 6mm×10mm.

[0018] Compared with the existing technology, the advantages of the utility model are:

[0019] 1. Compared with the traditional manual centrifuge tube sample addition and dilution, the sample dilution device described in the present application only adds sample once during the dilution process, and there is no need to use a pipette for subsequent transfer. The complete dilution operation is controlled by rotating the cover part, which simplifies the operation steps.

[0020] 2. Compared with the traditional manual centrifuge tube sample addition and dilution, the dilution device in the present application only adds sample once during the dilution process. The entire device can process 5-10 samples simultaneously with high throughput. When performing large-scale sample operations, especially when testing process development samples in biomedical laboratories, it can save a lot of time and labor costs.

[0021] 3. Compared with traditional manual centrifuge tube sample dilution or large pipetting workstations, especially for nucleic acid testing projects, traditional manual centrifuge tube sample dilution or large pipetting workstations will have aerosol contamination between different samples in the same space, which will lead to reduced accuracy of the test results. The dilution device in the present application only adds samples once during the dilution process, and no subsequent pipetting operations and lid opening operations are performed. The dilution of all samples is completed in their respective liquid flow carrier paths, achieving complete physical isolation, avoiding cross-contamination between samples and cross-contamination and confusion between different dilution steps of the same sample.

[0022] 4. The dilution device in the present application uses a rotating cover component as a valve, and the precise size design of the liquid transfer channel realizes microfluidics of sample dilution of 100uL, ensuring that the sample is accurately diluted at a ratio of 100:900.

[0023] 5. The main body of the dilution device in the present application is made of transparent plastic, which can monitor the liquid flow in the device in real time to ensure that the sample dilution is accurate and effective. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a cross-sectional view of the main body of the dilution device in the utility model;

[0025] Figure 2 It is a schematic diagram of the structure of the rotating cover component in the utility model;

[0026] Figure 3 It is a top view of the main body of the dilution device in the utility model;

[0027] Figure 4 It is a side cross-sectional view of the main body of the dilution device of the utility model after adding a sample to the 10-fold dilution liquid well;

[0028] Figure 5 It is a top view of the main body of the dilution device of the utility model after adding a sample to the 10-fold dilution liquid well;

[0029] Figure 6It is a side cross-sectional view of the main body of the dilution device of the utility model after adding sample diluent and sample into the liquid well and covering with a rotating cover component;

[0030] Figure 7 It is a top view of the main body of the dilution device of the utility model after adding sample diluent and sample into the liquid well and covering with a rotating cover component;

[0031] Figure 8 It is a side cross-sectional view of the main body of the dilution device after the rotating cover component corresponding to the 10-fold dilution liquid well is screwed to the second gear, and the main body of the dilution device is tilted 90 degrees to accurately transfer 100uL of the mixed solution to the liquid transfer channel;

[0032] Fig. 9 It is a top view of the main body of the dilution device after the rotating cover component corresponding to the 10-fold dilution liquid well is screwed to the second gear, and the main body of the dilution device is tilted 90 degrees to accurately transfer 100uL of the mixed solution to the liquid transfer channel;

[0033] Fig.10 It is a side cross-sectional view of the main body of the dilution device in the present invention, in which the rotating cover component 2 corresponding to the 10-fold dilution liquid filling well is turned to the third gear to cut off the liquid reflux;

[0034] Fig.11 It is a top view of the main body of the dilution device in the present invention, in which the rotating cover component 2 corresponding to the 10-fold dilution liquid filling well is turned to the third gear to cut off the liquid reflux;

[0035] Fig.12 The utility model is a side cross-sectional view of the main body of the dilution device, in which the rotating cover component 2 corresponding to the 100-fold dilution liquid well is turned to the second gear, so that the liquid in the channel flows into the 100-fold dilution liquid well;

[0036] Fig.13 The main body of the dilution device is shown in the top view when the rotating cover component 2 corresponding to the 100-fold dilution liquid well is turned to the second gear, so that the liquid in the channel flows into the 100-fold dilution liquid well;

[0037] In the figure: dilution device body 1, rotating cover rotation positioning arrow 11, digital mark 12, sample identification area 13, sample column partition mark 14, dilution multiple mark 15, rotating cover component 2, plate rack 3, liquid filling well 4, upper end 41, lower end 42, 900uL quality control scale line 421, liquid transfer channel 5, rotating cover body 6, liquid transfer port 61, rotating handle 7, rotating cover direction indication mark 71. DETAILED DESCRIPTION

[0038] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0039] like Figure 1-3 As shown, a sample multiple dilution device for quantitative detection includes a dilution device body 1 and a rotating cover component 2. The dilution device body 1 includes a plate rack 3. The height range of the plate rack 3 is 25mm-35mm, preferably 30mm, the thickness of the plate rack 3 is 3mm-5mm, preferably 4mm, and the width of the plate rack is 95mm. The plate rack 3 serves as the skeleton of the dilution device body 1 and supports the entire device. Therefore, the entire dilution device body 1 is designed with support plate racks all around.

[0040] A plurality of liquid wells 4 are provided on the plate frame 3, and a liquid transfer channel 5 for connecting the liquid wells 4 is provided on the plate frame 3; the rotating cover component 2 includes a rotating cover body 6 arranged in the liquid well 4, a rotating handle 7 is provided on the rotating cover body 6, and a liquid transfer port 61 capable of connecting with the liquid transfer channel 5 is provided on the side wall of the rotating cover body 6.

[0041] The dilution device body 1 is designed with four U-shaped liquid filling wells 4, each of which includes an upper end 41 and a lower end 42, and the inner diameter of the upper end 41 is larger than the inner diameter of the lower end 42, and the inner diameter of the upper end 41 is consistent with the outer diameter of the rotating cover component 2, both of which are 9.66 mm; the inner diameter of the lower end 42 of the liquid filling well 4 is consistent with the inner diameter of the rotating cover component 2, both of which are 9.1 mm. The design purpose is to maintain the seal when the rotating cover component 2 is covered on the liquid filling well 4, while ensuring that the internal side walls of the entire liquid filling well 4 are at the same height, reducing the liquid flow resistance and promoting the smooth inflow of liquid.

[0042] In addition, since the application scenario of the dilution device body 1 in the present application is for 10-fold dilution of 1:9, and the most typical ratio is 100:900, the liquid filling well 4 is designed to be able to hold a volume of more than 1000uL of liquid, the upper end 41 of the liquid filling well 4 is designed to be 8mm in height, and the lower end 42 is designed to be 14mm in height, and the liquid filling well 4 is a U-shaped well with a full opening at the upper end.

[0043] In order to accurately control the volume of the remaining liquid in the sample dilution mixture and ensure the accuracy of the dilution, the main body 1 of the dilution device of the present application is provided with a 900uL quality control scale line 421 at the lower end 42 of the liquid well 4. If it is found that the remaining mixed liquid is higher than the 900uL quality control scale line 421 during the dilution process, it means that the volume of the liquid transferred to the liquid transfer channel 5 is less than 100uL, and the sample dilution is too large and inaccurate; on the contrary, if it is found that the remaining mixed liquid is lower than the 900uL quality control scale line 421, it means that there is leakage or liquid sticking to the rotating cover component 2, and the liquid needs to be shaken off.

[0044] The bottom of the liquid-filling well 4 is designed to be a U-shaped bottom or a V-shaped bottom, preferably a V-shaped bottom; the V-shaped bottom design is more conducive to effective mixing of the liquid.

[0045] In addition, a circular opening is designed on one side of the upper end of the liquid well 4 close to the liquid transfer channel 5, and the opening diameter is 3.0mm-4.0mm, preferably 3.6mm. The purpose of the opening design is to transfer the mixed dilution liquid unidirectionally between the liquid wells 4 through the liquid transfer channel 5.

[0046] The function of the liquid transfer channel 5 is to accurately transfer the diluted mixed liquid in each liquid well 4 to the liquid well 4 in the next step; the basis for accurate transfer is that the liquid transfer channel 5 controls the total liquid volume to be accurately 100uL, and its inner diameter is designed to be 3.0mm-4.0mm, preferably 3.6mm; the corresponding liquid transfer channel 5 length is designed to be 9mm-10mm, preferably 9.83mm; the preferred size design avoids the situation where the surface tension of the channel is too narrow to generate an air column, thereby causing inaccurate liquid transfer. Although the size design avoids surface tension, in order to further reduce this risk, a hydrophilic treatment layer is provided on the circumferential inner wall of the liquid transfer channel 5 to ensure smooth liquid flow. The hydrophilic treatment layer is oxygen ion, ultraviolet / ozone, etc.

[0047] The function of the rotating cover component 2 is to serve as a "valve" for the liquid transfer in the liquid well 4 on the dilution device body 1, ensuring that the liquid is effectively transferred to the liquid well 4 of the next dilution step. In order to achieve the above function, the rotating cover body 6 is designed to be composed of a rotating cover body 6 and a rotating handle 7. The rotating cover body 6 is designed to be hollow inside, with the upper end sealed and the lower end completely open, and the side wall is designed with a liquid transfer port 61; and the inner diameter of the rotating cover body 6 is 9.1mm, the outer diameter is 9.66mm, and the opening diameter is 3.0mm-4.0mm, preferably 3.6mm. In addition, a rotating handle 7 is designed on the top of the rotating cover body 6, and the rotating handle 7 is parallel to the opening of the liquid transfer port 61, and a rotating cover direction indicator 71 is marked on the side of the rotating handle 7 facing the opening of the liquid transfer port 61. The design purpose of the rotating cover direction indicator 71 is to guide the operator to accurately rotate the rotating cover component 2 during the dilution process to ensure the effective transfer of the diluted mixed liquid.

[0048] The upper end surface of the dilution device body 1 and the circumference of the liquid well 4 are provided with a rotational positioning arrow 11 for the rotary cover and a digital mark 12 for the rotational positioning of the rotary cover in different dilution steps. In addition, on the upper end surface of the dilution device body 1 and the side of the liquid well 4 located at the head, there are respectively marked with A, B, C sample column partition marks 14 for indicating different channels, and the side of the liquid well 4 is marked with 10, 100, 1000, 10000 dilution multiple marks 15 for determining the dilution operation sequence from left to right. The purpose of this design is to guide the operator to judge the dilution of each step of the liquid well 4 and the dilution sequence.

[0049] In order to facilitate the marking of the diluted sample information, a sample identification area 13 for filling in the sample information with a marker is provided on the right side of the liquid filling well at the tail of the dispensing device body 1. The size of the sample identification area 13 is 6 mm×10 mm.

[0050] Because the device is used to load liquid flow carriers, the entire device is designed to be made of transparent material. The design purpose is to be used for real-time observation of liquid flow conditions, such as the 900uL quality control scale line 421. The specific material is polycarbonate or polystyrene, preferably polycarbonate.

[0051] Embodiment 1

[0052] Specific use process of the multiple dilution device:

[0053] ① Add 900uL of sample diluent to each of the four liquid wells 4, and add 100uL of the sample to be diluted to the 10-fold dilution liquid well 4. Figure 4-5 As shown;

[0054] ② Place the four rotating cover parts 2 in Figure 6-7 The cover is shown on the liquid filling well 4, that is, all the rotation positioning arrows 11 of the rotating cover point to the first gear.

[0055] ③ Mix the device on a mixer for 5 seconds;

[0056] ④Turn the rotating cover part 2 of the 10-fold dilution liquid well 4 to the ② position, such as Figure 8-9 As shown, the entire diluting device is tilted 90 degrees so that the mixed liquid fills the liquid transfer channel 5;

[0057] ⑤Turn the rotating cover component 2 of the 10-fold dilution well 4 to the ③th gear, such as Figure 10-11 As shown, the liquid reflux is cut off, and 100uL of the diluted mixed solution is stored in the liquid transfer channel 5;

[0058] ⑥ Such as Figure 12-13 As shown, the rotating cover component 2 corresponding to the 100-fold dilution liquid well 4 is turned to the second gear, so that the liquid in the liquid transfer channel 5 flows into the 100-fold dilution liquid well 4, and 100uL of liquid is accurately transferred from the 10-fold dilution mixed liquid into the 100-fold dilution liquid well 4. The subsequent dilution operation from 100-1000-1000 is the same as the above steps, and the overall dilution step can be completed by only two simple operations of twisting the rotating cover component 2 and mixing.

[0059] Embodiment 2

[0060] Comparison of test results between manual dilution and dilution device body 1:

[0061] This implementation case compares the dilution accuracy and time of manual dilution and dilution device main body 1 by testing the physical titer of 3 upstream lentivirus samples. The ELISA kit (Lentivirus Titer p24 ELISA Kit) used was purchased from GenScript, with the item number: L00938. For specific detection steps, please refer to the kit manual. Since ELISA detection technology is already a mature technology in the industry, it will not be repeated here. The 3 upstream lentivirus samples were provided by the company's PD virus laboratory.

[0062]

[0063] Table 1

[0064] The test results are shown in Table 1. The three chronic disease test results show that the dilution sample results of the dilution device body 1 have the same accuracy as manual dilution.

[0065] In summary, the principle of this embodiment is: 900uL of sample diluent is added to the four liquid-filling wells 4 respectively, and the rotating cover parts 2 of the 10000, 1000 and 100-fold dilution liquid-filling wells 4 are covered and tightly closed at the same time, and it is ensured that the direction of the rotating cover direction indicator mark 71 on the rotating handle 7 of the rotating cover part 2 points to the 10-fold dilution liquid-filling well 4, and the arrow of the rotating cover direction indicator mark 71 faces forward. 100uL of the sample liquid to be diluted is added to the 10-fold dilution liquid-filling well 4, and the rotating cover part 2 is covered, and the rotating cover direction indicator mark 71 is consistent with the rotating cover rotation positioning arrow 11 of the liquid-filling well 4, and points to the 100-fold dilution liquid-filling well 4. After rotating and mixing the liquid in the 10-fold dilution liquid well 4 for 5 seconds, the rotating cover component 2 is screwed to the next gear, and the entire device is rotated 90 degrees, so that 100uL of the mixed liquid is accurately transferred to the liquid transfer channel 5, and the rotating cover component 2 of the 10-fold dilution liquid well 4 is screwed to the next gear again to close the liquid transfer channel 5. At the same time, the rotating cover direction indicator 71 on the 100-fold dilution liquid well 4 is screwed to the next gear, so that the mixed liquid flows into the 100-fold dilution liquid well 4 for mixing and dilution, and so on, to complete the entire dilution step.

[0066] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

[0067] Although this article uses more terms such as diluting device body 1, rotating cover rotation positioning arrow 11, digital mark 12, sample mark area 13, sample column partition mark 14, dilution multiple mark 15, rotating cover component 2, plate rack 3, liquid well 4, upper end 41, lower end 42, 900uL quality control scale line 421, liquid transfer channel 5, rotating cover body 6, liquid transfer port 61, rotating handle 7, rotating cover direction indication mark 71, etc., it does not exclude the possibility of using other terms. The use of these terms is only to more conveniently describe and explain the essence of the utility model; interpreting them as any additional restrictions is contrary to the spirit of the utility model.

Claims

1. A sample multiple dilution device for quantitative detection, comprising a dilution device body (1) and a rotating cover component (2), characterized in that: The dilution device body (1) comprises a plate frame (3), the plate frame (3) is provided with a plurality of liquid wells (4), and the plate frame (3) is provided with a liquid transfer channel (5) for connecting with the liquid wells (4); the rotating cover component (2) comprises a rotating cover body (6) arranged in the liquid well (4), the rotating cover body (6) is provided with a rotating handle (7), and the side wall of the rotating cover body (6) is provided with a liquid transfer port (61) that can be connected with the liquid transfer channel (5).

2. A sample multiple dilution device for quantitative detection according to claim 1, characterized in that: The rotating cover body (6) is cylindrical and hollow inside, the upper end of the rotating cover body (6) is sealed and the lower end is completely open, and the rotating handle (7) is arranged parallel to the opening of the liquid transfer port (61) as a whole.

3. A sample dilution device for quantitative detection according to claim 2, characterized in that: The side of the rotating handle (7) facing the opening of the liquid transfer port (61) is marked with a rotating cover direction indication mark (71).

4. A sample multiple dilution device for quantitative detection according to claim 2, characterized in that: The liquid transfer port (61) is a circular port, and the opening diameter of the liquid transfer port (61) ranges from 3.0 mm to 4.0 mm. The inner diameter of the rotating cover body (6) ranges from 9.0 mm to 9.2 mm, and the outer diameter ranges from 0.65 mm to 9.67 mm. The height of the plate frame (3) ranges from 25 mm to 35 mm, and the thickness of the plate frame (3) ranges from 3 mm to 5 mm.

5. The sample dilution device for quantitative detection according to claim 1, characterized in that: The liquid-filling well (4) comprises an upper end (41) and a lower end (42), the inner diameter of the upper end is larger than the inner diameter of the lower end (42), the liquid-filling well (4) is a U-shaped well with a fully open upper end, and a circular opening is provided on a side of the upper end (41) of the liquid-filling well (4) close to the liquid transfer channel (5), and the opening diameter is 3.0 mm-4.0 mm.

6. A sample multiple dilution device for quantitative detection according to claim 5, characterized in that: A 900uL quality control scale line (421) is provided on the outside of the lower end portion (42) of the liquid-filling well (4), and a hydrophilic treatment layer is provided on the circumferential inner wall of the liquid transfer channel (5).

7. A sample multiple dilution device for quantitative detection according to claim 6, characterized in that: The bottom shape of the lower end portion (42) of the liquid-filling well (4) is V-shaped or U-shaped.

8. The sample multiple dilution device for quantitative detection according to claim 1, characterized in that: The upper end surface of the dilution device body (1) and located in the circumferential direction of the liquid storage well (4) are provided with a rotary cover rotation positioning arrow (11) and a number mark (12) for the rotary cover rotation positioning in different dilution steps.

9. The sample multiple dilution device for quantitative detection according to claim 1, characterized in that: The upper end surface of the dilution device body (1) and the liquid-filling well (4) located at the head are respectively marked with A, B, and C sample column partition marks (14) for indicating different channels, and the side surfaces of the liquid-filling well (4) are marked with 10, 100, 1000, and 10000 dilution multiple marks (15) from left to right for determining the dilution operation sequence.

10. The sample multiple dilution device for quantitative detection according to claim 1, characterized in that: A sample identification area (13) for filling in sample information with a marker pen is provided on one side of the liquid-filling well (4) at the tail end of the dilution device body (1).

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