Positive pressure swing culture system

Abstract

The utility model relates to the technical field of medical culture equipment, in particular to a positive pressure swing culture system, which comprises a closed box body, a mounting plate is fixedly mounted at one end of the bottom of the box body, a plurality of culture bottles communicated with the mounting plate are fixed in the mounting plate, a gas input end is arranged on the box body, and a gas outlet end is arranged on the gas input end. The other side of the gas input end is hermetically connected with a gas washing bottle arranged in the box body through a gas pipe, and the gas outlet end of the gas washing bottle is communicated with the mounting disc through a gas pipe. By controlling and adjusting the pressure and temperature of the culture environment and driving the culture bottle to move eccentrically, the natural growth environment of the embryonic cells in a living body is simulated, so that the growth and development of the embryonic cells are promoted, and the culture efficiency of the embryonic cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical culture equipment, in particular to a positive pressure swing culture system. Background Art

[0002] In the medical field, during the culture process of embryonic cells, the stability of the cell growth environment and the nutrient supply are important factors affecting cell growth. Traditional embryonic cell culture systems often only focus on the formulation of the culture medium and the control of environmental factors such as temperature and humidity, but ignore the state of embryonic cells in the natural growth environment of the organism. Therefore, there is still room for improvement in the efficiency and quality of embryonic cell culture.

[0003] In view of this, we propose a positive pressure swing culture system to solve the existing problems. Utility Model Content

[0004] The purpose of the utility model is to provide a positive pressure swing culture system to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a positive pressure swing culture system, comprising a closed box, a mounting plate is fixedly installed at one end of the interior of the box, a plurality of culture bottles connected to the mounting plate are fixed in the mounting plate, a gas input end is provided on the box, the other side of the gas input end is sealed and connected to a gas washing bottle arranged inside the box through an air pipe, and the gas outlet end of the gas washing bottle is connected to the mounting plate through the air pipe.

[0006] Preferably, a driving motor is also fixed in the box body, and a transmission device is provided at the inner bottom end of the box body. The output end of the driving motor is drivingly connected to the input end of the transmission device, and a crankshaft driven to rotate by the driving motor is installed at the end of the transmission device away from the driving motor. A support member is provided at the bottom center end of the mounting plate, and the support member rotates in cooperation with the crankshaft through a bearing.

[0007] Preferably, the transmission device has a plurality of drive connection holes distributed on the same virtual extension line at one end away from the drive motor, and the crankshaft can be detachably installed in any one of the drive connection holes, and drives the mounting plate to swing back and forth along the drive connection hole under the control of the drive motor.

[0008] Preferably, the box body further comprises a heating port for heating the interior of the box body, and a temperature sensor is also fixed on the inner wall of the box body.

[0009] Preferably, a gas pressure regulating valve is provided between the gas input end and the gas washing bottle and / or between the gas washing bottle and the culture bottle.

[0010] Preferably, a support member is provided at the center of the mounting plate, and the support member is rotatably connected to the crankshaft via a bearing.

[0011] Compared with the prior art, the beneficial effect of the utility model is that the system of the utility model simulates the natural growth environment of embryonic cells in the organism by controlling and adjusting the pressure and temperature of the culture environment and driving the culture bottle to perform eccentric movement, thereby promoting the growth and development of embryonic cells and improving the culture efficiency of embryonic cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a front view structural schematic diagram of the utility model;

[0013] Figure 2 It is a schematic diagram of the top view of the drive motor, crankshaft, transmission device and drive connecting hole of the utility model.

[0014] In the figure: 1. temperature sensor; 2. driving motor; 3. gas washing bottle; 4. culture bottle; 5. mounting plate; 6. supporting member; 7. heating port; 8. crankshaft; 9. transmission device; 10. driving connection hole. DETAILED DESCRIPTION

[0015] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and specific embodiments.

[0016] Embodiment 1

[0017] like Figure 1 and Figure 2 As shown, a positive pressure swing culture system proposed by the utility model comprises a closed box body, a mounting plate 5 is fixedly mounted at one end inside the box body, a plurality of culture bottles 4 connected with the mounting plate 5 are fixed in the mounting plate 5, a gas input end is provided on the box body, the other side of the gas input end is sealedly connected with a gas washing bottle 3 arranged inside the box body through an air pipe, and the gas outlet end of the gas washing bottle 3 is connected with the mounting plate 5 through the air pipe.

[0018] In the embodiment, a driving motor 2 is also fixed in the box, and a transmission device 9 is provided at the bottom end of the box. The output end of the driving motor 2 is drivingly connected to the input end of the transmission device 9. A crankshaft 8 driven to rotate by the driving motor 2 is installed at the end of the transmission device 9 away from the driving motor 2. A support member 6 is provided at the bottom center end of the mounting plate 5, and the support member 6 rotates in cooperation with the crankshaft 8 through a bearing.

[0019] In the embodiment, a plurality of drive connection holes 10 are distributed on the same virtual extension line at one end of the transmission device 9 away from the drive motor 2. The crankshaft 8 can be detachably installed in any one of the drive connection holes 10 and drives the mounting plate 5 to swing back and forth along the drive connection hole 10 under the control of the drive motor 2.

[0020] In the embodiment, the box body further comprises a heating port 7 for heating the interior of the box body, and a temperature sensor 1 is also fixed on the inner wall of the box body.

[0021] In the embodiment, a gas pressure regulating valve is provided between the gas input end and the gas washing bottle 3 and / or between the gas washing bottle 3 and the culture bottle 4 .

[0022] In the embodiment, a support member 6 is provided at the center of the mounting plate 5 , and the support member 6 is rotatably connected to the crankshaft 8 via a bearing.

[0023] The working principle of a positive pressure swing culture system based on the first embodiment is: when working, the culture bottle 4 containing the embryo is fixedly installed in the installation disk 5, and the external mixed gas is transported to the washing bottle 3 placed inside the box through the trachea through the gas input end arranged on the box body, and the gas is moistened by the washing bottle 3 and input to the installation disk 5 through the output end of the washing bottle 3, and is distributed to the culture bottle 4 through the installation disk 5. During the process, the gas pressure in the culture circuit in the entire box is adjusted by the gas pressure regulating valve, so as to simulate the pressure from the amniotic fluid that the embryo bears in the uterus, and the inside of the box is heated through the heating port 7 and the temperature is adjusted. The sensor 1 is used to accurately record the ambient temperature in the control box, so as to ensure that the embryo in the culture bottle 4 can grow and develop in a suitable temperature and pressure environment. At the same time, the driving motor 2 drives the crankshaft 8 installed on the driving connection hole to move through the transmission device 9. The crankshaft 8 drives the mounting plate 5 to make constant eccentric movement through the support member 6. The support member 6 is connected to the crankshaft 8 through a bearing, so that the mounting plate 5 has the freedom to rotate during the swinging movement, thereby avoiding tracheal entanglement, so that the embryonic cells in the culture bottle 4 can be suspended in the culture solution, maintain 360° contact with the surrounding culture solution, and promote the growth of the embryo.

[0024] The above-mentioned specific embodiments are only several preferred embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A positive pressure swing culture system, comprising a closed box, characterized in that: A mounting plate (5) is fixedly mounted at one end of the interior of the box, and a plurality of culture bottles (4) connected to the mounting plate (5) are fixed in the mounting plate (5). A gas input end is provided on the box, and the other side of the gas input end is sealedly connected to a gas washing bottle (3) arranged inside the box through an air pipe, and the gas outlet end of the gas washing bottle (3) is connected to the mounting plate (5) through the air pipe.

2. A positive pressure swing culture system according to claim 1, characterized in that: A drive motor (2) is also fixed in the housing, a transmission device (9) is provided at the bottom end of the housing, an output end of the drive motor (2) is drivingly connected to an input end of the transmission device (9), a crankshaft (8) driven to rotate by the drive motor (2) is installed at one end of the transmission device (9) away from the drive motor (2), a support member (6) is provided at one end at the bottom center of the mounting plate (5), and the support member (6) rotates in cooperation with the crankshaft (8) via a bearing.

3. A positive pressure swing culture system according to claim 2, characterized in that: A plurality of drive connection holes (10) are distributed on the same virtual extension line at one end of the transmission device (9) away from the drive motor (2); the crankshaft (8) is detachably mounted in any one of the drive connection holes (10) and drives the mounting plate (5) to swing back and forth along the drive connection hole (10) under the control of the drive motor (2).

4. A positive pressure swing culture system according to claim 1, characterized in that: The box body also includes a heating port (7) for heating the interior of the box body, and a temperature sensor (1) is fixed on the inner wall of the box body.

5. The positive pressure swing culture system according to claim 1, characterized in that: A gas pressure regulating valve is provided between the gas input end and the gas washing bottle (3) and / or between the gas washing bottle (3) and the culture bottle (4).

6. A positive pressure swing culture system according to claim 1, characterized in that: A support member (6) is provided at the center of the mounting plate (5), and the support member (6) is rotatably connected to the crankshaft (8) via a bearing.

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