Light source capable of being used for cell imager

Abstract

The utility model discloses a light source for a cell imager. The light source comprises a light source shell, a cylindrical light source lamp, an annular light source lamp and a beam splitter prism, the light source shell comprises a bottom plate, the cylindrical light source lamp is arranged above the bottom plate, the annular light source lamp is arranged below the bottom plate, the beam splitter prism is arranged between the bottom plate and the cylindrical light source lamp, and light emitted by the cylindrical light source lamp vertically penetrates through the beam splitter prism and then is converged with light emitted by the annular light source lamp. According to the light source capable of being used for the cell imager disclosed by the utility model, when light rays emitted by the cylindrical light source lamp pass through the beam splitter prism, the beam splitter prism can effectively control diffraction of the light rays and reduce or avoid unnecessary light ray diffusion and interference, so that the definition and the accuracy of imaging are kept; and the beam splitter prism can reflect and refract the incident light according to a specific angle, so that the light cannot be greatly lost, the high efficiency of light path transmission is maintained, the light energy loss is favorably reduced, and the light path transmission efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of microscopes, in particular to a light source that can be used for a cell imaging instrument. Background Art

[0002] A live cell imager is an instrument used to observe and record the dynamic changes of living cells in biological processes. It usually combines high-resolution imaging technology, fluorescence microscopy technology, and computer image processing technology, and can capture images of cells at different time points in real time, and study and analyze cell structure, function, and activity. In the prior art, a cylindrical light source (parallel light illumination) is generally used to illuminate the sample to obtain a uniform sample image and clear contours and edges; or an annular light source (oblique illumination) is used to illuminate the sample to make the contour of the sample surface more obvious and the three-dimensional sense stronger. In this regard, those skilled in the art combine a cylindrical light source with an annular light source to obtain a uniform sample image and clear contours and edges while increasing the three-dimensional sense of the sample. However, if the light generated by the two light sources is simply collected and illuminated to the sample, the light is easily diffused and interfered during the collection process, which reduces the focus and clarity of the light, resulting in unsatisfactory lighting effects, and the contours and details of the sample surface cannot be clearly displayed.

[0003] In view of this, it is necessary to improve the light source of the existing cell imager to increase the outline clarity of the sample. Utility Model Content

[0004] The purpose of the utility model is to provide a light source that can be used for a cell imaging instrument, so as to solve the technical problem in the prior art that the light from a cylindrical light source lamp and a ring light source lamp is simply gathered and illuminated to a sample, and the light is easily diffused and disturbed in the process of gathering, so that the focus and clarity of the light are reduced, resulting in an unsatisfactory lighting effect and the contour and details of the sample surface cannot be clearly displayed.

[0005] To achieve this purpose, the utility model adopts the following technical solutions:

[0006] A light source that can be used in a cell imaging instrument, comprising:

[0007] A light source housing, the light source housing comprising a bottom plate, the bottom plate being provided with a first through hole;

[0008] An annular light source lamp is arranged below the bottom plate;

[0009] A cylindrical light source lamp is arranged above the bottom plate, and the light emitted by the cylindrical light source lamp can pass through the first through hole and converge with the light emitted by the annular light source lamp;

[0010] The beam splitter prism is arranged between the bottom plate and the cylindrical light source lamp. The light emitted by the cylindrical light source lamp vertically passes through the beam splitter prism and then converges with the light emitted by the ring light source lamp.

[0011] Optionally, the light source that can be used for the cell imaging instrument also includes an optical fiber for emitting laser light, and the emitting end of the optical fiber is horizontally directed toward the beam splitter prism, so that the laser light emitted by the optical fiber can vertically pass through the first through hole after being refracted by the beam splitter prism.

[0012] Optionally, a support frame is provided at the periphery of the beam splitter prism, the beam splitter prism is arranged in the inner cavity of the support frame, a plurality of fixing rods are provided on the support frame, a fixing plate is provided on the fixing rods, and the cylindrical light source lamp is fixed on the fixing plate.

[0013] Optionally, an adapter plate is provided on the base plate, a plurality of first fine-adjustment holes are provided on the adapter plate, and second fine-adjustment holes corresponding to the first fine-adjustment holes are provided on the base plate, so that the support frame can be adjusted and installed on the base plate through the adapter plate.

[0014] Optionally, a fiber optic fine-tuning mechanism is provided on one side of the support frame, and the fiber optic fine-tuning mechanism is connected to the support frame via a connecting rod.

[0015] Optionally, the optical fiber fine-tuning mechanism includes a shell, a cover body, a fine-tuning hole ring and a fine-tuning knob arranged in a accommodating cavity formed by the shell and the cover body, and two adjacent side surfaces of the shell are provided with fine-tuning holes passing through the accommodating cavity, and the fine-tuning knob passes through the fine-tuning hole to fine-tune the distance of the fine-tuning hole ring in the horizontal and vertical directions.

[0016] Optionally, the optical fiber fine-tuning mechanism also includes an elastic support block, which is arranged on two adjacent inner walls of the accommodating cavity and located on two inner walls opposite to the fine-tuning hole. The elastic support block is connected to the inner wall of the accommodating cavity through a spring to generate an elastic supporting force on the fine-tuning hole ring.

[0017] Optionally, the light source that can be used for the cell imaging instrument also includes a fixed angle code and a light source support column, one side of the fixed angle code is connected to the base plate, and the other side is connected to the light source support column, and an adjustable lifting block is provided on the side of the fixed angle code connected to the light source support column, and the fixed angle code is connected to the light source support column through the lifting block.

[0018] Compared with the prior art, the utility model has the following beneficial effects: when working, the cylindrical light source lamp and the annular light source lamp are turned on at the same time, and the light emitted by the cylindrical light source lamp is vertically incident on the dichroic prism and vertically emitted from the dichroic prism, that is, the light emitted by the cylindrical light source lamp passes vertically through the dichroic prism, and then is collected with the light emitted by the annular light source lamp outside the light source housing to illuminate the sample. The light source that can be used for a cell imaging instrument disclosed in the utility model patent, when the light emitted by the cylindrical light source lamp passes through the dichroic prism, the dichroic prism can effectively control the diffraction of the light, reduce or avoid unnecessary light diffusion and interference, thereby maintaining the clarity and accuracy of the imaging; and the dichroic prism can reflect and refract the incident light at a specific angle, so that the light will not be lost significantly, and the high efficiency of the light path transmission is maintained, which helps to reduce the loss of light energy and improve the efficiency of the light path transmission. At the same time, the dichroic prism can effectively protect the internal cylindrical light source to avoid interference or damage to the light source by the external environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0020] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with this technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantive technical significance. Any structural modification, change in proportion or adjustment of size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical contents disclosed by the present invention.

[0021] Figure 1 This is a schematic diagram of the structure of the utility model patent installed on the base of the cell imaging instrument;

[0022] Figure 2 This is a schematic diagram of the internal structure of a light source that can be used for a cell imaging instrument according to the utility model patent;

[0023] Figure 3 This is an exploded view of the optical fiber fine-tuning mechanism of the utility model patent.

[0024] Illustrations: 10. Light source housing; 11. Bottom plate; 12. Decorative strip; 20. Ring light source lamp; 30. Cylindrical light source lamp; 40. Beam splitter prism; 50. Optical fiber; 60. Support frame; 61. Fixing rod; 62. Fixing plate; 63. Adapter plate; 64. First fine-tuning hole; 70. Optical fiber fine-tuning mechanism; 71. Connecting rod; 72. Shell; 73. Cover; 74. Fine-tuning hole ring; 75. Fine-tuning knob; 76. Fine-tuning hole; 77. Elastic support block; 78. Spring; 81. Fixed angle code; 82. Light source support column; 83. Lifting block; 100. Cell imaging instrument base. DETAILED DESCRIPTION

[0025] In order to make the purpose, features and advantages of the invention of the utility model more obvious and easy to understand, the technical scheme in the embodiment of the utility model will be clearly and completely described below in combination with the drawings in the embodiment of the utility model. Obviously, the embodiment described below is only a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] In the description of the present invention, it should be understood that the terms "upper", "lower", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. It should be noted that when a component is considered to be "connected" to another component, it may be directly connected to the other component or there may be a centrally arranged component at the same time.

[0027] The technical solution of the utility model is further explained below with reference to the accompanying drawings and through specific implementation methods.

[0028] Reference Figures 1 to 3 The embodiment of the utility model provides a light source that can be used for a cell imager, characterized in that it includes a light source housing 10, an annular light source lamp 20, a cylindrical light source lamp 30 and a dichroic prism 40. The annular light source lamp 20 is arranged outside the light source housing 10, and the cylindrical light source lamp 30 and the dichroic prism 40 are arranged inside the light source housing 10. The light source housing 10 includes a bottom plate 11, and the light source that can be used for a cell imager also includes a light source support column 82, the light source housing 10 is installed on the light source support column 82 through the bottom plate 11, and the light source support column 82 is installed on the cell imager base 100. A decorative strip 12 is arranged on the outer side of the light source housing 10.

[0029] The bottom plate 11 is provided with a first through hole, the annular light source lamp 20 is arranged below the bottom plate 11, and the cylindrical light source lamp 30 is arranged above the bottom plate 11. The light emitted by the cylindrical light source lamp 30 can pass through the first through hole and be collected together with the light emitted by the annular light source lamp 20. Specifically, the cylindrical light source lamp 30 emits light above the bottom plate 11, and the annular light source lamp 20 emits light below the bottom plate 11. After the light emitted by the cylindrical light source lamp 30 passes through the first through hole, it is collected together with the light emitted by the annular light source lamp 20 below the bottom plate 11. Optionally, the axis of the cylindrical light source lamp 30, the axis of the first through hole, and the axis of the annular light source lamp 20 are on the same straight line.

[0030] The beam splitter prism 40 is disposed between the bottom plate 11 and the cylindrical light source lamp 30 , so that the light from the cylindrical light source lamp 30 vertically passes through the beam splitter prism 40 and converges with the light from the ring light source lamp 20 .

[0031] It should be noted that the beam splitter prism 40 is in a cubic shape and is formed by combining two right-angle prisms of the same shape, wherein the inclined surface of the right-angle prism forms an angle of 45 degrees with the horizontal plane.

[0032] When working, the cylindrical light source lamp 30 and the annular light source lamp 20 are turned on at the same time, and the light emitted by the cylindrical light source lamp 30 is vertically injected into the dichroic prism 40, and vertically emitted from the dichroic prism 40, that is, the light emitted by the cylindrical light source lamp 30 passes through the dichroic prism 40 vertically, and then is collected with the light emitted by the annular light source lamp 20 outside the light source housing 10 to illuminate the sample. The light source that can be used for cell imaging instruments disclosed in the utility model patent, when the light emitted by the cylindrical light source lamp 30 passes through the dichroic prism 40, the dichroic prism 40 can effectively control the diffraction of the light, reduce or avoid unnecessary light diffusion and interference, so as to maintain the clarity and accuracy of the imaging; and the dichroic prism 40 can reflect and refract the incident light at a specific angle, so that the light will not be lost significantly, and the high efficiency of the light path transmission is maintained, which helps to reduce the loss of light energy and improve the efficiency of the light path transmission. At the same time, the dichroic prism 40 can effectively protect the internal cylindrical light source to avoid interference or damage to the light source by the external environment.

[0033] It should be noted that the cylindrical light source lamp 30 is formed in a cylindrical shape, and the annular light source lamp 20 is a circular ring with a narrow upper end and a wide lower end, and LED lamp beads are arranged on the inner side wall of the circular ring.

[0034] A support frame 60 is disposed on the periphery of the beam splitter prism 40, and the beam splitter prism 40 is disposed in the inner cavity of the support frame 60. A plurality of fixing rods 61 are disposed on the support frame 60, and a fixing plate 62 is disposed on the fixing rods 61. The cylindrical light source lamp 30 is fixed on the fixing plate 62. The upper and lower sides of the support frame 60 are provided with second through holes penetrating the inner cavity, so that the light emitted by the cylindrical light source lamp 30 can pass through the support frame 60.

[0035] The bottom plate 11 is provided with an adapter plate 63, and a plurality of first fine adjustment holes 64 are provided on the adapter plate 63. The bottom plate 11 is provided with second fine adjustment holes corresponding to the first fine adjustment holes 64, so that the support frame 60 can be adjusted and installed on the bottom plate 11 through the adapter plate 63. During installation, the support frame 60 is first installed on the adapter plate 63, and then the screw is passed through the first fine adjustment hole 64 and the second fine adjustment hole, and the nut is rotated and installed into one end of the screw. When the nut and the screw are fully tightened, the relative position of the support frame 60 and the bottom plate 11 is adjusted, and then the nut and the screw are tightened.

[0036] The light source that can be used for the cell imaging instrument also includes an optical fiber 50 for emitting laser light. Specifically, the optical fiber 50 is connected to a laser for emitting a laser line. The emitting end of the optical fiber 50 is horizontally directed toward the beam splitter prism 40, so that the laser line emitted by the optical fiber 50 is horizontally incident on the beam splitter prism 40, and the laser line is refracted by the beam splitter prism 40 and then vertically emitted from the beam splitter prism 40, and then vertically passes through the first through hole to irradiate the sample.

[0037] An optical fiber fine-tuning mechanism 70 is provided on one side of the support frame 60, and the optical fiber fine-tuning mechanism 70 is connected to the support frame 60 through a connecting rod 71. The optical fiber fine-tuning mechanism 70 includes a shell 72, a cover 73, a fine-tuning hole ring 74 and a fine-tuning knob 75 provided in a receiving cavity formed by the shell 72 and the cover 73, two adjacent side surfaces of the shell 72 are provided with fine-tuning holes 76 penetrating the receiving cavity, and the fine-tuning knob 75 passes through the fine-tuning hole 76 to fine-tune the movement of the fine-tuning hole ring 74 in the horizontal and vertical directions. The optical fiber 50 is provided on the axis of the fine-tuning hole ring 74, so that when the movement of the fine-tuning hole ring 74 in the horizontal and vertical directions is adjusted, the movement of the optical fiber 50 in the horizontal and vertical directions can be adjusted.

[0038] The optical fiber fine-tuning mechanism 70 also includes an elastic support block 77, which is arranged on two inner side walls adjacent to the accommodating cavity and located on two inner side walls opposite to the fine-tuning hole 76. The elastic support block 77 is connected to the inner side wall of the accommodating cavity through a spring 78 to generate an elastic supporting force on the fine-tuning hole ring 74.

[0039] The light source that can be used for the cell imaging instrument also includes a fixed angle code 81, one side of the fixed angle code 81 is connected to the base plate 11, and the other side is connected to the light source support column 82. An adjustable lifting block 83 is provided on the side of the fixed angle code 81 connected to the light source support column 82, and the fixed angle code 81 is connected to the light source support column 82 via the lifting block 83.

[0040] It should be further explained that the light source that can be used for the cell imaging instrument disclosed in the utility model has the following imaging modes: (1) The laser emitted by the optical fiber 50 is refracted by the dichroic prism 40 and illuminates the sample to form laser quantitative phase imaging, so that the light spot is uniform and does not damage the cells; (2) The parallel light formed by the cylindrical light source lamp 30 alone illuminates the sample to obtain a uniform sample image and clear contours and edges; (3) The light formed by the ring light source lamp 20 illuminates the sample to make the contour of the sample surface more obvious and the three-dimensional effect stronger. (4) The light emitted by the cylindrical light source lamp 30 and the ring light source lamp 20 are combined to illuminate the sample to obtain a clear sample image with a strong three-dimensional effect.

[0041] As described above, the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. A light source that can be used for a cell imaging instrument, characterized in that: include: A light source housing (10), the light source housing (10) comprising a bottom plate (11), the bottom plate (11) being provided with a first through hole; An annular light source lamp (20) is arranged below the bottom plate (11); A cylindrical light source lamp (30) is arranged above the bottom plate (11), and the light emitted by the cylindrical light source lamp (30) can pass through the first through hole and converge with the light emitted by the annular light source lamp (20); The beam splitter prism (40) is arranged between the bottom plate (11) and the cylindrical light source lamp (30); the light emitted by the cylindrical light source lamp (30) vertically passes through the beam splitter prism (40) and converges with the light emitted by the annular light source lamp (20).

2. The light source for cell imaging according to claim 1, characterized in that: It also comprises an optical fiber (50) for emitting laser light, wherein the emission end of the optical fiber (50) is horizontally directed toward the beam splitter prism (40), so that the laser light emitted by the optical fiber (50) can vertically pass through the first through hole after being refracted by the beam splitter prism (40).

3. The light source for cell imaging according to claim 1, characterized in that: A support frame (60) is arranged on the periphery of the beam splitter prism (40), the beam splitter prism (40) is arranged in the inner cavity of the support frame (60), a plurality of fixing rods (61) are arranged on the support frame (60), a fixing plate (62) is arranged on the fixing rods (61), and the cylindrical light source lamp (30) is fixed on the fixing plate.

4. The light source for cell imaging according to claim 3, characterized in that: An adapter plate (63) is provided on the base plate (11), a plurality of first fine adjustment holes (64) are provided on the adapter plate, and second fine adjustment holes corresponding to the first fine adjustment holes (64) are provided on the base plate (11), so that the support frame (60) can be adjusted and installed on the base plate (11) through the adapter plate (63).

5. The light source for cell imaging according to claim 3, characterized in that: An optical fiber fine-tuning mechanism (70) is provided on one side of the support frame (60), and the optical fiber fine-tuning mechanism (70) is connected to the support frame (60) via a connecting rod (71).

6. The light source for cell imaging according to claim 5, characterized in that: The optical fiber fine-tuning mechanism (70) comprises a shell (72), a cover (73), a fine-tuning hole ring (74) and a fine-tuning knob (75) arranged in a receiving cavity formed by the shell (72) and the cover (73); two adjacent side surfaces of the shell (72) are provided with fine-tuning holes (76) penetrating the receiving cavity; the fine-tuning knob (75) passes through the fine-tuning hole (76) to fine-tune the distance of the fine-tuning hole ring (74) in the horizontal and vertical directions.

7. The light source for cell imaging according to claim 6, characterized in that: The optical fiber fine-tuning mechanism (70) further comprises an elastic support block (77), wherein the elastic support block (77) is arranged on two inner side walls adjacent to the accommodating cavity and located on two inner side walls opposite to the fine-tuning hole (76), and the elastic support block (77) is connected to the inner side wall of the accommodating cavity via a spring (78) to generate an elastic support force on the fine-tuning hole ring (74).

8. The light source for cell imaging according to claim 1, characterized in that: It also comprises a fixed angle bracket (81) and a light source support column (82); one side of the fixed angle bracket (81) is connected to the bottom plate (11), and the other side is connected to the light source support column (82); an adjustable lifting block (83) is provided on the side of the fixed angle bracket (81) connected to the light source support column (82); and the fixed angle bracket (81) is connected to the light source support column (82) via the lifting block (83).

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