Magnetoelastic aortic dissection immune sensing device

Abstract

The utility model provides a magnetoelastic aortic dissection immune sensing device which comprises a blood collecting device, a magnetoelastic detecting device and a magnetoelastic sensor chip testing rod, the blood collecting device comprises a blood collecting tube, a storage tube and a centrifugal tube which are respectively connected with a blood collecting needle, and the blood collecting device is used for collecting blood; the centrifugal tube can be used for carrying out centrifugal treatment on blood; the magnetoelastic detection device is provided with an integrated circuit and an induction coil device, and a magnet is arranged above the magnetoelastic detection device; the magnetoelastic sensor chip test rod is positioned in a test rod socket in the top of the magnetoelastic detection device and is provided with a plurality of grooves, and the grooves are used for placing magnetoelastic sensors and blood samples. The blood collection device and the magnetoelastic detection equipment are combined, blood collection, storage, centrifugation and detection are completed in one device, detection can be carried out immediately after blood collection, the sampling efficiency is high, the structure is simple, power consumption is small, carrying is convenient, operation is simple, and real-time monitoring can be achieved.

Description

Technical Field

[0001] The utility model relates to a detection device, in particular to a magnetoelastic aortic dissection immune sensor device. Background Art

[0002] Acute aortic dissection (AAD) is a serious cardiovascular emergency caused by a rupture in the aortic wall intima. It often has a sudden onset, severe course, high mortality and is accompanied by multiple complications. The age structure of the potential AAD population is mainly middle-aged and elderly people. In addition, patients with hypertension, hereditary diseases and aortic aneurysms are at a higher risk of AAD. Currently, the main method for diagnosing AAD in clinical practice is imaging examination, including computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound examination (including ultrasound Doppler and echocardiography). These methods rely on large instruments in hospitals, which are costly and difficult to move. AAD patients must go to the hospital for imaging examinations. At present, there are the following problems in the diagnosis of AAD in clinical practice: (1) The treatment is not timely enough. AAD treatment needs to be carried out immediately to prevent the dissection from continuing to expand and the aorta from rupturing, causing massive bleeding; (2) The cost of long-term treatment and examination is high. The end of AAD treatment does not mean complete recovery. Patients need long-term treatment and follow-up to prevent the recurrence of AAD and further damage to the aorta, which is time-consuming and costly; (3) Traditional diagnostic methods may have adverse effects on patients. For example, CTA examinations require contrast agents, which pose risks of allergies and renal damage to patients. The occurrence of AAD can cause a rapid increase in the content of certain proteins in the human body in a short period of time, such as DD (D-Dimer) and CRP (C-reactive protein). Therefore, detecting the content of these disease markers can assist in determining whether AAD has occurred and provide timely diagnosis and treatment for AAD. Currently, the commonly used protein detection methods in clinical practice have limitations such as high cost and long experimental time. Therefore, there is an urgent need for a real-time rapid detection device.

[0003] In recent years, with the rapid development of new sensor systems, magnetoelastic sensors have become the focus of biosensor research due to their advantages of rapidity, high sensitivity, good specificity and low cost, and are widely used in precision medicine, biochemical testing and environmental monitoring. Under the action of the alternating magnetic field of the induction coil and the bias magnetic field provided by the magnet, the biochemical reaction on the surface of the magnetoelastic sensor is used to detect the resonance frequency deviation of the magnetoelastic sensor to reflect the concentration of the target detection object, thereby assisting the diagnosis of diseases (such as AAD).

[0004] The detection principle is to modify the surface of the magnetoelastic sensor with the corresponding antibody of the target detection object, and then add the detection object. The detection object and its antibody specifically bind to the surface of the magnetoelastic sensor, thereby causing the load mass of the magnetoelastic sensor to change, and then generating the resonant frequency deviation of the magnetoelastic sensor, thereby reflecting the concentration of the target detection object. The resonant frequency of the bare magnetoelastic sensor is shown in formula (1), where L is the length of the magnetoelastic chip, ρ is the chip density, σ is the Poisson's ratio of the chip, and E is the Young's modulus. The resonant frequency of the magnetoelastic sensor after loading is shown in formula (2), where Δm is the mass load and m0 is the mass of the bare sensor. The resonant frequency deviation is shown in formula (3).

[0005]

[0006]

[0007] Utility Model Content

[0008] The utility model provides a magnetoelastic aortic dissection immune sensor device, which solves the problem of combining a blood collection device and a magnetoelastic detection device for auxiliary diagnosis of acute aortic dissection based on the blood collection and centrifugation principle and the detection principle of a magnetoelastic sensor. The technical solution is as follows:

[0009] A magnetoelastic aortic dissection immune sensor device comprises a blood collection device, a magnetoelastic detection device and a magnetoelastic sensor chip test rod, wherein the blood collection device comprises a blood collection tube, a storage tube and a centrifuge tube respectively connected to a blood collection needle and used to collect blood, and the centrifuge tube can perform centrifugal treatment on the blood; the magnetoelastic detection device is provided with an integrated circuit and an induction coil device, and a magnet is provided on the top; the magnetoelastic sensor chip test rod is located at the internal test rod socket of the top of the magnetoelastic detection device, and is provided with a plurality of grooves, and the grooves are used to place the magnetoelastic sensor and the blood sample;

[0010] The induction coil device includes an induction coil and a transparent shell. The induction coil is wound around the surface of the transparent shell of the cuboid embedded in the outer cylinder. The groove corresponds to the induction coil. The magnetoelastic sensor works under the action of the alternating magnetic field of the induction coil and the bias magnetic field provided by the magnet controlled by the magnet control arm.

[0011] The blood collection device is provided with a temperature sensing circuit and a motor driving module; the storage tube realizes temperature measurement through the temperature sensing circuit and displays it on a display screen outside the storage tube; the centrifuge tube realizes shaft rotation through a motor to separate blood cells and plasma, and the motor is driven by the motor driving module.

[0012] The integrated circuit includes a micro control unit circuit provided with a control chip, and a direct frequency digital synthesizer, a Bluetooth module, a storage module, a power supply circuit and a WIFI module respectively connected to the micro control unit circuit; the induction coil device is connected to the micro control unit circuit through an amplifier follower circuit and an RMS-DC conversion circuit; and the direct frequency digital synthesizer is connected to the induction coil device.

[0013] The control chip used in the microcontroller circuit is APM32F103RCT6, and the eight pins PA4, PA5, PA6, PA7, PC6, PC7, PC8, and PC9 of the control chip are connected to a direct digital frequency synthesizer AD9958;

[0014] The four pins PC0, PC1, PC2 and PC3 of the control chip are connected to the AD converter AD736;

[0015] The PC10 and PC11 pins of the control chip are connected to the Bluetooth module HLk-B40;

[0016] The four pins PB3, PB4, PC12 and PD2 of the control chip are connected to the WT-01S WIFI module ESP8266;

[0017] The PB12, PB13, PB14, and PB15 pins of the control chip are connected to W25Q32 of the storage module;

[0018] The PA10 pin of the control chip is connected to the temperature sensing circuit DS18B20S;

[0019] The PC4, PC5, PB0, PB1, PA8, and PA9 pins of the control chip are connected to the motor drive module L298N;

[0020] The PB10 and PB11 pins of the control chip are connected to the display module HS96L03;

[0021] The PA1 and PA2 pins of the control chip are connected to the LED lamp;

[0022] The PA15, PB7, PB8, and PB9 pins of the control chip are connected to the key switches M1, M2, M3, and M4; the PA11 and PA12 pins are connected to the key switches TS1 and TS2; the PB5 and PB6 pins are connected to the knob switches SW1 and SW2;

[0023] The PD0 and PD1 pins of the control chip are connected to an 8 MHz crystal oscillator.

[0024] The chip used in the direct frequency digital synthesizer is AD9958. Pins 50 and 51 of the chip are connected to the microcontroller circuit for secure digital input and output. Pins 8 and 13 of the chip are two-way signal outputs.

[0025] The chip used in the amplifier-follower circuit is LM324DT, which amplifies and processes the induced current of the induction coil. The chip used in the RMS-DC conversion circuit is AD736. The amplifier-follower circuit is connected to the RMS-DC conversion circuit and transmits the measurement result to the control chip through the OUTPUT pin.

[0026] The storage module uses chip W25Q32, the Bluetooth module 8 uses chip U11 HLk-B40, the display module uses chip HS96L03, and the WiFi module uses chip WT-01S WIFI module ESP8266.

[0027] The temperature sensing circuit adopts the chip DS18B20S, and transmits the temperature data to the control chip through the DQ pin. The two key switches TS1 and TS2 control the temperature display and the unit conversion respectively.

[0028] The chip used in the motor drive module is L298N, whose pins 5 and 7 control the rotation direction of one motor, pins 10 and 12 control the rotation direction of another motor, pin 6 controls the speed of one motor, pin 11 controls the speed of another motor, and the timing and constant speed of the motor are controlled by knobs SW1 and SW2.

[0029] The voltage regulator chip used in the power supply circuit is AMS1117, and the power supply voltage conversion chip is R-78E5, which provides -5V, +5V, +1.8V, +3.3V, and +12V voltages for the overall detection device.

[0030] The beneficial effects of the utility model compared with the prior art are:

[0031] The magnetoelastic aortic dissection immune sensor device combines a blood collection device with a magnetoelastic detection device, so that blood collection, storage, centrifugation and detection can be completed in one device, avoiding the waste of blood samples when transferring blood from multiple devices. The device can be tested immediately after blood collection, which has good timeliness and high sampling efficiency. The overall structure of the device is simple, power consumption is low, it is easy to carry, operation is simple, and real-time monitoring is possible. The four-channel design of the magnetoelastic detection device effectively improves the detection efficiency. The blood collection needle and blood collection hose in the blood collection device are replaceable to avoid cross infection and effectively improve safety. The data obtained from the test can also be sent to the mobile phone for display via Bluetooth to achieve wireless transmission. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1ais a front view schematic diagram of the magnetoelastic aortic dissection immunosensing device;

[0033] Figure 1b is a top view schematic diagram of the magnetoelastic aortic dissection immunosensing device;

[0034] Figure 1c is a side schematic diagram of the magnetoelastic aortic dissection immunosensing device;

[0035] Figure 2 is a diagram of the internal structure of the blood collection device;

[0036] Figure 3 is a schematic diagram of the operating interface of the detection device;

[0037] Figure 4a is a schematic diagram of the induction coil device;

[0038] Figure 4b is a schematic diagram of the magnetoelastic sensor test rod;

[0039] Figure 5 is a schematic diagram of a circuit framework of the detection device;

[0040] Figure 6 is a schematic diagram of the micro control unit circuit;

[0041] Figure 7 is a schematic diagram of the direct frequency digital synthesizer circuit;

[0042] Figure 8 is a schematic diagram of the amplifier-follower and RMS-DC conversion circuit;

[0043] Fig. 9 is a circuit diagram of the storage module;

[0044] Fig.10 is a schematic diagram of the Bluetooth transmission circuit;

[0045] Fig.11 is a schematic diagram of the temperature sensing circuit;

[0046] Fig.12 is a schematic diagram of the motor drive module;

[0047] Fig.13 is a circuit diagram of the display module;

[0048] Fig.14 is a circuit diagram of the WiFi module;

[0049] Fig.15 is a schematic diagram of the power supply circuit. DETAILED DESCRIPTION

[0050] The utility model provides a magnetoelastic aortic dissection immune sensor device, which is used to combine a blood collection device and a magnetoelastic detection device to achieve auxiliary diagnosis of AAD. Figures 1a to 1c As shown, the utility model includes a blood collection device 1, a magnetoelastic detection device 2 and a magnetoelastic sensor chip test rod 3, wherein the blood collection device 1 includes a blood collection tube, a storage tube and a centrifuge tube respectively connected to a blood collection needle for collecting blood, and the centrifuge tube can centrifuge the blood; the magnetoelastic detection device 2 is provided with a magnet, an integrated circuit and an induction coil device, and the integrated circuit is connected to the induction coil device; the magnetoelastic sensor chip test rod 3 is provided with a plurality of grooves, and the grooves are used to place the magnetoelastic sensor and the blood sample. The present invention utilizes the biochemical reaction on the surface of the magnetoelastic sensor under the action of the alternating magnetic field of the induction coil and the bias magnetic field provided by the magnet, and reacts the concentration of the target detection object by detecting the resonance frequency deviation of the magnetoelastic sensor.

[0051] Combination Figure 2 As shown, the blood collection tube 1c, storage tube 1d, and centrifuge tube 1e are independent of each other, and the blood stored in the tube can be added to the test rod of the magnetoelastic detection device through the blood collection needle 1a for detection. The external blood collection needle 1a and the hose 1b connected to the blood collection needle 1a are disposable and replaceable to prevent contamination of blood and cross infection of the human body. The blood collection needle 1a can be connected to the blood collection tube 1c, storage tube 1d, and centrifuge tube 1e respectively through the blood collection hose 1b and the bottom interface (1l, 1m, 1n). The three tubes of the blood collection tube 1c, storage tube 1d, and centrifuge tube 1e are all provided with a piston rod part 1f. Furthermore, the blood collection device 1 has a built-in temperature sensor circuit 12 and a motor drive module 13.

[0052] The blood collection tube 1c uses a blood collection needle 1a to collect blood from the human body. The negative pressure difference formed between the blood collection tube 1c and the blood vessel causes the blood to be collected into the blood collection tube 1c. A glass window is used on the outside of the blood collection tube 1c to visualize the collected blood volume.

[0053] The storage tube 1d uses an insulating layer to achieve short-term storage, and is provided with a display screen 1g on the outside. A button control area 1h is provided on the front of the device, and the blood temperature in the storage tube can be read in real time through button control. The temperature measurement is achieved through a temperature sensing circuit 12. The storage tube can store blood for a short period of time, and its external display screen can monitor the internal temperature at any time through button control, which helps to prevent the blood from being qualitatively changed due to temperature changes.

[0054] The outside of the centrifuge tube is provided with a knob control area 1j, and the knob outside the centrifuge tube controls the centrifugation time and speed. The inside of the centrifuge tube is a detachable part 1k. After the centrifuge tube 1e is connected to a power source, the motor inside it generates torque and electromagnetic force to rotate the centrifuge tube shaft, and the blood is affected by the centrifugal force and rotates outward away from the shaft, thereby achieving separation of blood cells and plasma in the blood. The motor is driven by a motor drive module 13.

[0055] The blood collection tube 1c, storage tube 1d, and centrifuge tube 1e are connected by the bottom button control area 1i. Furthermore, the blood collection tube 1c and storage tube 1d, and the storage tube 1d and centrifuge tube 1e are connected by the bottom button control, so that the blood just collected from the human body is processed in an integrated manner, and it is also convenient to clean the blood in an integrated manner after the test is completed. The centrifuge tube is detachable, which is convenient for drying and cleaning wastewater treatment.

[0056] When in use, pull the piston rod on the top of the multifunctional blood collection device to collect, store and centrifuge blood, or pull the piston rod on the top of the blood collection tube to collect blood, and then control the buttons at the bottom of the device to make the collected blood enter the storage tube for short-term storage and the centrifuge tube for centrifugation in turn, and finally the blood is stored in the internal tube of the blood collection device.

[0057] like Figure 3 As shown, the operation interface of the magnetoelastic detection device 2 is provided with a button control area 14, a display light area 15 and a display screen 16 on the outside of the device. A magnet control arm 2b is provided above the magnetoelastic detection device 2, and the magnet control arm 2b is integrally connected to the magnetoelastic detection device 2 through an omnidirectional knob 2a, so as to facilitate the search for the optimal bias magnetic field position during detection.

[0058] Combination Figure 5 As shown, the magnetoelastic detection device has a built-in integrated circuit, including a micro control unit circuit 7, and a direct frequency digital synthesizer 4, a Bluetooth module 8, a storage module 9, a power supply circuit 10 and a WIFI module 11 respectively connected to the micro control unit circuit 7, the induction coil device is connected to the micro control unit circuit 7 through an amplifier follower circuit 5 and an RMS-DC conversion circuit 6, and the direct frequency digital synthesizer 4 is connected to the induction coil device. Further, the micro control unit circuit 7 is connected to the keys of the key control area 14, the display lights of the display light area 15 and the display screen 16.

[0059] The microcontrol unit circuit 7 is the core circuit of the magnetoelastic detection device. After the system is powered on, the power indicator light is on, and the microcontrol unit circuit 7 is in a state of waiting for control instructions. When a key is pressed, the signal generating circuit of the direct frequency digital synthesizer 4 receives the control instruction issued by the microcontrol unit circuit 7 to generate a sinusoidal excitation signal, and then the induction coil a of the induction coil device generates an alternating magnetic field. Under the action of the alternating magnetic field generated by the induction coil a and the bias magnetic field generated by the magnet, the magnetoelastic sensor of the magnetoelastic sensor chip test rod 3 generates a frequency deviation due to the immune binding reaction on its surface. The response information of the induction coil a is converted into a signal that can be received by the microcontrol unit circuit 7 through the amplification follower circuit 5 and the RMS-DC conversion circuit 6, thereby completing the collection of the response information, sending it to the memory for storage through the data bus, and finally displaying the drawing curve on the display screen 16, or the Bluetooth module 8 is successfully connected to the smart phone end through the key control, the Bluetooth indicator light is on, and the response information is sent to the smart phone end via the Bluetooth module 8 for display.

[0060] The magnetoelastic detection device has the characteristics of parallel detection of multiple samples without mutual interference. It controls Bluetooth switch, data sending, data sending stop and curve graph display through buttons, and uses indicator lights to respectively display power on and successful Bluetooth connection.

[0061] like Figure 4a and 4b As shown, the induction coil device includes an induction coil a and a transparent shell b, the magnetoelastic sensor chip test rod 3 is located inside the top of the magnetoelastic detection device 2, a test rod socket is provided on the right side of the magnetoelastic detection device 2 as a whole, the induction coil a is wound around the surface of the transparent shell b of the rectangular parallelepiped embedded in the outer cylinder, the magnetoelastic test rod c is provided with four equally spaced grooves d for placing the magnetoelastic sensor e, the grooves correspond to the coil positions one by one, a handle d is provided at the rightmost end of the test rod, and the test rod is inserted into the test rod socket of the magnetoelastic detection device 2 by holding the handle d, and then inserted into the induction coil a. The direct frequency digital synthesizer 4 and the amplifier follower circuit 5 are respectively connected to the induction coil a through wires, and the magnetoelastic sensor e works under the action of the alternating magnetic field of the induction coil a and the bias magnetic field provided by the magnet controlled by the magnet control arm 2b above the device.

[0062] The induction coil device as a whole adopts an outer cylinder and an inner rectangular parallelepiped structure, and the magnetoelastic sensor chip test rod 3 adopts a rectangular parallelepiped structure. The outer cylinder makes the wound coil not easy to break or damage, and the inner rectangular parallelepiped can make the inserted test rod more stable, avoiding the test rod flipping and causing the sample to pour out, causing pollution and sample waste. The magnetoelastic sensor e is placed in the groove part of the test rod. There are multiple grooves on the test rod, which can measure multiple samples at the same time or measure one sample multiple times at the same time, so that the accuracy of the device is increased.

[0063] When the utility model is used, the blood collection device 1 realizes integrated processing of blood collection, storage and centrifugation: when blood collection starts from a human body, the piston rod at the top of the blood collection tube is pulled to collect blood into the blood collection tube, and after the blood collection needle and the hose are removed, the connection between the blood collection tube and the outside world is closed by controlling the bottom button. In general, the three tubes are kept in a closed state to ensure the sealing inside the blood collection device. At this time, the blood collection tube and the storage tube are connected by the bottom button of the device, the piston rod at the top of the storage tube is pulled, and the blood enters the storage tube for storage. The piston rod of the blood collection tube is pulled back by force, and then the connection between the two tubes is closed by the bottom button of the device. The external display screen of the storage tube performs real-time temperature display and unit conversion (Celsius and Fahrenheit), and the storage tube part adopts a temperature sensor circuit and a display module circuit. After the blood is stored in the storage tube, the storage tube and the centrifuge tube are connected by the bottom button of the device, the piston rod at the top of the centrifuge tube is pulled, and the blood enters the centrifuge tube. The piston rod at the top of the storage tube is pulled back by force, and the connection between the two tubes is closed by the bottom button. At this time, centrifugation is controlled by the knob, and the centrifuge tube part adopts a motor drive module.

[0064] The corresponding antibodies of AAD disease markers are modified on the surface of the magnetoelastic sensor as a magnetoelastic immunosensor. The blood of potential AAD patients is collected using a blood collection device, and the blood is added to the groove of the test rod where the magnetoelastic immunosensor is placed using a blood collection needle. If an immune binding reaction occurs on the surface of the immunosensor, and its response curve reaction group is offset compared to the blank group and reaches the set value, it means that the person who collected the blood is very likely to suffer from AAD.

[0065] Select three disease markers of AAD, use four magnetoelastic sensors of exactly the same size, one of which is used as a blank group, and the other three magnetoelastic sensors with corresponding antibodies modified with the target detection object are used as reaction groups. They are placed in multiple grooves of the test rod in turn, and the blood to be tested is added to the multiple grooves respectively using a blood collection needle and a blood collection hose. The handle of the test rod is held, and then the whole is inserted into the induction coil through the socket on the right side of the device. A magnet is controlled by a magnet control arm. After connecting the power supply, the magnetoelastic sensor begins to vibrate under the action of the bias magnetic field generated by the magnet and the alternating magnetic field generated by the induction coil. The corresponding functions (Bluetooth switch, send data, stop sending data and curve display) are realized through the four button controls of the operation interface of the magnetoelastic detection device. According to the diagnostic criteria of AAD and the optimal detection concentration of its disease markers, the corresponding concentration calibration value is set, and the calibration value of the blank group is compared with the final response result of the reaction group to assist in judging whether the person whose blood is collected is a high-incidence patient of AAD or suffers from AAD. Finally, the test results are displayed on the display screen of the magnetoelastic detection device or sent to the mobile phone via Bluetooth for display.

[0066] like Figure 6As shown, the chip U1 used in the microcontroller circuit 7 of the present invention is APM32F103RCT6, which is used as the control chip of the system. The chip is based on - High-quality domestic (Geehy Semiconductor) 32-bit general-purpose MCU with M0+ / M3 / M4 core, built-in high-speed memory, rich peripherals, can work normally in the temperature range of -40℃ to +105℃, and has the advantages of high integration, high performance, and low power consumption.

[0067] The circuit structure of the micro control unit circuit 7 is:

[0068] The eight pins PA4, PA5, PA6, PA7, PC6, PC7, PC8 and PC9 of the single-chip microcomputer chip U1 are connected to a direct digital frequency synthesizer AD9958;

[0069] The four pins PC0, PC1, PC2 and PC3 of the single-chip microcomputer chip U1 are connected to the AD converter AD736;

[0070] The PC10 and PC11 pins of the single-chip microcomputer chip U1 are connected to the Bluetooth module HLk-B40;

[0071] The four pins PB3, PB4, PC12 and PD2 of the single-chip microcomputer chip U1 are connected to the WT-01S WIFI module ESP8266;

[0072] The PB12, PB13, PB14, and PB15 pins of the single-chip microcomputer chip U1 are connected to W25Q32 of the storage module;

[0073] The PA10 pin of the single-chip microcomputer chip U1 is connected to the temperature sensing circuit DS18B20S;

[0074] The PC4, PC5, PB0, PB1, PA8, and PA9 pins of the single-chip microcomputer chip U1 are connected to the motor drive module L298N;

[0075] The PB10 and PB11 pins of the single-chip microcomputer chip U1 are connected to the display module HS96L03;

[0076] The PA1 and PA2 pins of the single-chip microcomputer chip U1 are connected to the LED lamp;

[0077] The PA15, PB7, PB8, and PB9 pins of the single-chip microcomputer chip U1 are connected to the key switches M1, M2, M3, and M4; the PA11 and PA12 pins are connected to the key switches TS1 and TS2; the PB5 and PB6 pins are connected to the knob switches SW1 and SW2;

[0078] The PD0 and PD1 pins of the single-chip microcomputer chip U1 are connected to an 8 MHz crystal oscillator.

[0079] In addition, the NRST pin is also connected to a reset circuit including a reset button.

[0080] like Figure 7 As shown, the chip used in the direct frequency digital synthesizer 4 of the utility model is AD9958, which is used to generate a swept frequency signal. The core of the chip AD9958 is two direct digital frequency synthesizers (DDS), each channel can provide independent frequency, phase and amplitude control, and integrates a high-speed 10-bit dual-channel DAC with narrow-band SFDR performance and excellent bandwidth. Each channel has a dedicated 10-bit output ratio multiplier, 14-bit phase offset and 32-bit frequency tuning word. DDS is used as a high-resolution divider, REFCLK is used as input, and DAC provides output. The REFCLK input source is common to both channels and can be directly driven or used in combination with an integrated REFCLK multiplier (PLL), with a maximum of 500MSPS. The chip can work normally in the temperature range of -40℃ to +85℃. AD9958 can generate storage waveforms with adjustable amplitude, adopts SPI communication protocol, communicates through the data lines SCLK, SIDO, SDO and CS, and supports amplitude modulation through an external DAC. In addition, AD9958 also has two clock input pins for providing the clock signal of the chip, namely CLKP and CLKN, and the maximum input clock frequency is 180MHz. The utility model adopts an 8MHz clock input and is controlled by the SPI serial clock line SCK of the microcontroller. The REF_CLK pin is connected to an external crystal oscillator. Pins 50 and 51 of the chip are connected to the microcontroller for secure digital input and output. Pins 8 and 13 of the chip are two-way signal outputs, and through two chips U2 and U4, a total of four signal outputs are realized through the coil, one of which is used for reference calibration samples, and the other three outputs are used for testing target detection objects.

[0081] The direct digital frequency synthesizer 4 is provided with two identical groups of U2 and U4, and the circuit structure is as follows:

[0082] Pin 3 of the control chip U2 is connected to pin PA7 of the control chip U1;

[0083] Pin 4 of the control chip U2 is connected to pin PA6 of the control chip U1;

[0084] Pin 46 of the control chip U2 is connected to pin PA3 of the control chip U1;

[0085] Pin 47 of the control chip U2 is connected to pin PA4 of the control chip U1;

[0086] Pin 48 of the control chip U2 is connected to pin PA5 of the control chip U1;

[0087] Pin 50 of the control chip U2 is connected to pin PC6 of the control chip U1;

[0088] Pin 51 of the control chip U2 is connected to pin PC7 of the control chip U1;

[0089] Pin 3 of the control chip U4 is connected to pin PA7 of the control chip U1;

[0090] Pin 4 of the control chip U4 is connected to pin PA6 of the control chip U1;

[0091] Pin 46 of the control chip U4 is connected to pin PA3 of the control chip U1;

[0092] Pin 47 of the control chip U4 is connected to pin PA4 of the control chip U1;

[0093] Pin 48 of the control chip U4 is connected to pin PA5 of the control chip U1;

[0094] Pin 50 of the control chip U4 is connected to pin PC8 of the control unit U1;

[0095] The 51st pin of the control chip U4 is connected to the PC9 pin of the control chip U1.

[0096] like Figure 8 As shown, the chip used in the amplifier follower circuit 5 is LM324DT, which is a true differential input of a four-channel operational amplifier, and has the characteristics of short-circuit output protection, single power supply (3.0V~32V), internal compensation, etc. The chip used in the RMS-DC conversion circuit is AD736, which converts the DC voltage waveform into a true RMS, average rectification, or an absolute value 200mV RMS full-scale input range after converting the AC and DC voltage waveforms (when an input attenuator is used, the input range is larger). The AD736 reading error bandwidth is only 1%, and for an input amplitude of 20~200mV RMS, the error bandwidth exceeds 10kHz; the power consumption is only 1mW. In the utility model, the chip LM324DT is the main body of the amplifier follower circuit, which amplifies and processes the induced current of the induction coil and then transmits it to the chip AD736, and the AD736 transmits the measurement results to the control chip through the OUTPUT pin.

[0097] The chip used in the amplifier follower circuit 5 is LM324, and the chips U6, U7, U8, and U9 used in the RMS-DC conversion circuit 6 are AD736, which processes the signal and transmits it to the micro control unit circuit 7. The circuit structure of the amplifier follower circuit 5 and the RMS-DC conversion circuit 6 is as follows:

[0098] Pin 6 of the control chip U6 is connected to pin PC0 of the control chip U1;

[0099] Pin 6 of the control chip U7 is connected to pin PC1 of the control chip U1;

[0100] Pin 6 of the control chip U8 is connected to pin PC2 of the control chip U1;

[0101] Pin 6 of the control chip U9 is connected to pin PC3 of the control chip U1.

[0102] like Fig. 9 As shown, the storage module adopts the chip W25Q32. The serial flash memory of this chip provides a solution for systems with limited pins, power and space, and the current consumption when powered off is as low as 1μA.

[0103] The utility model adopts the chip to store response data.

[0104] The circuit structure of the storage module 9 is:

[0105] Pin 1 of the control chip U10 is connected to pin PB12 of the control chip U1;

[0106] Pin 2 of the control chip U10 is connected to pin PB15 of the control unit U1;

[0107] Pin 5 of the control chip U10 is connected to pin PB14 of the control unit U1;

[0108] Pin 6 of the control chip U10 is connected to pin PB13 of the control unit U1.

[0109] like Fig.10 As shown, the chip U11 used by the Bluetooth module 8 is HLk-B40, which is based on BLE5.1, has a faster speed and a longer transmission distance, which can reach 40 to 100 meters in an open environment. The utility model sends the final measurement data results to the smart phone via Bluetooth.

[0110] The circuit structure of the Bluetooth module 8 is:

[0111] Pin 23 of the control chip U11 is connected to pin PC10 of the control unit U1;

[0112] Pin 24 of the control chip U11 is connected to pin PC11 of the control unit U1.

[0113] like Fig.11 As shown, the temperature sensing circuit of the utility model adopts the chip DS18B20S, which is a digital thermometer, which provides 9-12bit resolution temperature, the temperature measurement range is -55℃~+125℃ (-67℉~+257℉) and the measurement accuracy is ±0.4℃ in the range of -10℃~70℃. The utility model transmits temperature data to the control chip through the DQ pin, and the two key switches TS1 and TS2 control the temperature display and unit conversion respectively. The circuit structure of the temperature sensing circuit 12 is:

[0114] Pin 2 of the control chip U12 is connected to pin PA10 of the control unit U1

[0115] like Fig.12 As shown, the chip U13 used in the motor drive module 13 is L298N, which is an integrated monolithic circuit and a high-voltage, high-current dual full-bridge driver designed to accept standard TTL logic levels and drive inductive loads. In the utility model, the U13 pins 5 and 7 of the chip L298N control the rotation direction of one motor, and the pins 10 and 12 control the rotation direction of another motor. The U13 pin 6 of the chip L298N controls the speed of one of the motors, and the pin 11 controls the speed of the other motor. The knobs SW1 and SW2 are used to control the timing and constant speed of the motor.

[0116] The circuit structure of the motor drive module 13 is:

[0117] Pin 5 of the control chip U13 is connected to pin PC4 of the control chip U1;

[0118] Pin 6 of the control chip U13 is connected to pin PA8 of the control chip U1;

[0119] Pin 7 of the control chip U13 is connected to pin PC5 of the control chip U1;

[0120] Pin 10 of the control chip U13 is connected to pin PB0 of the control chip U1;

[0121] Pin 11 of the control chip U13 is connected to pin PA9 of the control chip U1;

[0122] Pin 12 of the control chip U13 is connected to pin PB1 of the control chip U1;

[0123] Pin 2 of the control chip U13 is connected to the positive terminal of the motor 1;

[0124] Pin 3 of the control chip U13 is connected to the negative pole of the motor 1;

[0125] Pin 13 of the control chip U13 is connected to the positive terminal of the motor 2;

[0126] Pin 14 of the control chip U13 is connected to the negative terminal of motor 2.

[0127] like Fig.13 As shown, the chip U14 used in the display module is HS96L03, which is used to display the corresponding data. The display mode of this chip is passive matrix, the display color is monochrome (white), the driving load is 1 / 64 load, and it has the characteristics of low power consumption and good heat dissipation effect. In the utility model, the key switches M1, M2, M3, and M4 are used to realize the Bluetooth switch, send data, stop sending data, and display the curve graph. The circuit structure of the display module is:

[0128] Pin 3 of the control chip U14 is connected to pin PB10 of the control unit U1;

[0129] Pin 4 of the control chip U14 is connected to pin PB11 of the control unit U1.

[0130] like Fig.14 As shown, the chip U15 used by the WiFi module 11 is the WT-01S WIFI module ESP8266, which is used for communication. Its core processor is ESP8266, which is small in size and integrates the Tensilica L106 ultra-low power 32-bit micro MCU, with a 16-bit streamlined mode and a main frequency support of 80MHz and 160MHz. The module supports standard IEEE802.11 b / g / n protocols and a complete TCP / IP protocol stack.

[0131] The circuit structure of the WIFI module 11 is:

[0132] Pin 2 of the control chip U15 is connected to pin PB3 of the control chip U1;

[0133] Pin 3 of the control chip U15 is connected to pin PB4 of the control chip U1;

[0134] Pin 4 of the control chip U15 is connected to pin PD2 of the control chip U1;

[0135] Pin 5 of the control chip U15 is connected to pin PC12 of the control chip U1.

[0136] like Fig.15As shown, the voltage regulator chips U16, U17, and U18 used in the power supply circuit 10 are AMS1117, and the power supply voltage conversion chip U19 is R-78E5, which provides -5V, +5V, +1.8V, +3.3V, and +12V voltages for the overall detection device. The chip AMS1117 is a low-dropout linear regulator that uses fine-tuning technology to control the output voltage accuracy within 2%. The power supply voltage conversion chip R-78E5 provides a negative voltage (-5V) for the AD736. The chip has an efficiency of 95% and has the characteristics of output short-circuit protection.

[0137] The circuit structure of the power supply circuit 10 is:

[0138] The 12V voltage is converted into 3.3V voltage for the microcontroller, 3.3V and 1.8V voltage for the AD9958 of the excitation signal circuit, -5V and +5V voltage for the effective value-to-DC conversion circuit AD736, 3.3V voltage for the storage module W25Q32, 3.3V voltage for the Bluetooth HLK-B40, 5V voltage for the temperature sensor, 5V voltage for the display module HS96L03, and 3.3V voltage for the WIFI module WT-01S. Provide 12V voltage for the motor driver chip L298N.

[0139] The beneficial effects of the utility model compared with the prior art are:

[0140] The magnetoelastic aortic dissection immune sensor device combines a blood collection device with a magnetoelastic detection device, so that blood collection, storage, centrifugation and detection can be completed in one device, avoiding the waste of blood samples when transferring blood from multiple devices. The device can be tested immediately after blood collection, which has good timeliness and high sampling efficiency. The overall structure of the device is simple, power consumption is low, it is easy to carry, operation is simple, and real-time monitoring is possible. The four-channel design of the magnetoelastic detection device effectively improves the detection efficiency. The blood collection needle and blood collection hose in the blood collection device are replaceable to avoid cross infection and effectively improve safety. The data obtained from the test can also be sent to the mobile phone for display via Bluetooth to achieve wireless transmission.

Claims

1. A magnetoelastic aortic dissection immunosensor device, characterized in that: It comprises a blood collection device, a magnetoelastic detection device and a magnetoelastic sensor chip test rod, wherein the blood collection device comprises a blood collection tube, a storage tube and a centrifuge tube respectively connected to a blood collection needle and used for collecting blood, and the centrifuge tube can perform centrifugal treatment on the blood; the magnetoelastic detection device is provided with an integrated circuit and an induction coil device, and a magnet is provided on the top; the magnetoelastic sensor chip test rod is located at the internal test rod socket of the top of the magnetoelastic detection device, and is provided with a plurality of grooves, and the grooves are used for placing the magnetoelastic sensor and the blood sample; The induction coil device includes an induction coil and a transparent shell. The induction coil is wound around the surface of the transparent shell of the cuboid embedded in the outer cylinder. The groove corresponds to the induction coil. The magnetoelastic sensor works under the action of the alternating magnetic field of the induction coil and the bias magnetic field provided by the magnet controlled by the magnet control arm.

2. The magnetoelastic aortic dissection immunosensor device according to claim 1, characterized in that: The blood collection device is provided with a temperature sensing circuit and a motor driving module; the storage tube realizes temperature measurement through the temperature sensing circuit and displays it on a display screen outside the storage tube; the centrifuge tube realizes shaft rotation through a motor to separate blood cells and plasma, and the motor is driven by the motor driving module.

3. The magnetoelastic aortic dissection immunosensor device according to claim 1, characterized in that: The integrated circuit includes a micro control unit circuit provided with a control chip, and a direct frequency digital synthesizer, a Bluetooth module, a storage module, a power supply circuit and a WIFI module respectively connected to the micro control unit circuit; the induction coil device is connected to the micro control unit circuit through an amplifier follower circuit and an RMS-DC conversion circuit; and the direct frequency digital synthesizer is connected to the induction coil device.

4. The magnetoelastic aortic dissection immunosensor device according to claim 3, characterized in that: The control chip used in the microcontroller circuit is APM32F103RCT6, and the eight pins PA4, PA5, PA6, PA7, PC6, PC7, PC8, and PC9 of the control chip are connected to a direct digital frequency synthesizer AD9958; The four pins PC0, PC1, PC2 and PC3 of the control chip are connected to the AD converter AD736; The PC10 and PC11 pins of the control chip are connected to the Bluetooth module HLk-B40; The four pins PB3, PB4, PC12 and PD2 of the control chip are connected to the WT-01S WIFI module ESP8266; The PB12, PB13, PB14, and PB15 pins of the control chip are connected to W25Q32 of the storage module; The PA10 pin of the control chip is connected to the temperature sensing circuit DS18B20S; The PC4, PC5, PB0, PB1, PA8, and PA9 pins of the control chip are connected to the motor drive module L298N; The PB10 and PB11 pins of the control chip are connected to the display module HS96L03; The PA1 and PA2 pins of the control chip are connected to the LED lamp; The PA15, PB7, PB8, and PB9 pins of the control chip are connected to the key switches M1, M2, M3, and M4; the PA11 and PA12 pins are connected to the key switches TS1 and TS2; the PB5 and PB6 pins are connected to the knob switches SW1 and SW2; The PD0 and PD1 pins of the control chip are connected to an 8 MHz crystal oscillator.

5. The magnetoelastic aortic dissection immunosensor device according to claim 3, characterized in that: The chip used in the direct frequency digital synthesizer is AD9958. Pins 50 and 51 of the chip are connected to the microcontroller circuit for secure digital input and output. Pins 8 and 13 of the chip are two-way signal outputs.

6. The magnetoelastic aortic dissection immunosensor device according to claim 3, characterized in that: The chip used in the amplifier-follower circuit is LM324DT, which amplifies and processes the induced current of the induction coil. The chip used in the RMS-DC conversion circuit is AD736. The amplifier-follower circuit is connected to the RMS-DC conversion circuit and transmits the measurement result to the control chip through the OUTPUT pin.

7. The magnetoelastic aortic dissection immunosensor device according to claim 3, characterized in that: The storage module uses chip W25Q32, the Bluetooth module uses chip U11 HLk-B40, the display module uses chip HS96L03, and the WiFi module uses chip WT-01S WIFI module ESP8266.

8. The magnetoelastic aortic dissection immunosensor device according to claim 2, characterized in that: The temperature sensing circuit adopts the chip DS18B20S, and transmits the temperature data to the control chip through the DQ pin. The two key switches TS1 and TS2 control the temperature display and the unit conversion respectively.

9. The magnetoelastic aortic dissection immunosensor device according to claim 2, characterized in that: The chip used in the motor drive module is L298N, whose pins 5 and 7 control the rotation direction of one motor, pins 10 and 12 control the rotation direction of another motor, pin 6 controls the speed of one motor, pin 11 controls the speed of another motor, and the timing and constant speed of the motor are controlled by knobs SW1 and SW2.

10. The magnetoelastic aortic dissection immunosensor device according to claim 1, characterized in that: The voltage regulator chip used in the power supply circuit of the magnetoelastic aortic dissection immune sensor device is AMS1117, and the power supply voltage conversion chip is R-78E5, which provides -5V, +5V, +1.8V, +3.3V, and +12V voltages for the overall detection device.

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