Cell spinner bottle machine with full-automatic monitoring function
By designing a cell rotary bottle machine with fully automatic monitoring function, the rotation and rotation motor combined with a microscopic imaging module can be used to realize automated monitoring of the cell culture process, which solves the problems of contamination, infection and production batch differences caused by manual observation in traditional technology, and improves the stability and consistency of the product.
Patent Information
- Application Number
- CN202421272218.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-05
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2034-06-05
AI Technical Summary
Traditional cell flask transfer machines require experimental personnel to frequently enter the biological isolation room for manual observation, which poses a risk of contamination and infection, and inconsistent manual judgments lead to large differences between production batches, affecting the stability and consistency of vaccines and biological agents.
A cell rotary flask machine with fully automatic monitoring is designed, and the rotation and rotation of the cell culture flask is realized using a revolution stepper motor and a rotation stepper motor, and a microscopic imaging module is installed at the cell detection station to realize automatic monitoring of the cell culture process.
Automatic monitoring of the cell culture process is realized, reducing the frequency of experimenters entering the biological isolation room, reducing the risk of contamination and infection, ensuring product consistency, and avoiding the impact of manual observation on cell growth.
Smart Images
Figure CN222647996U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell bottle rotating machine with full-automatic monitoring. Background Art
[0002] Cell culture is also called cell cloning technology. With the continuous development of cell culture technology in the field of mass production of vaccines and biological preparations, the demand for obtaining a large number of cells, cell matrices or their metabolites through cell culture is increasing, and there is an urgent need to develop large-scale cell culture technology. The cells commonly used in the production of vaccines and certain biological preparations are adherent growth cells, so the large-scale culture technology of adherent growth cells has always attracted much attention. The cell culture rotary bottle machine, also known as the cell culture shaker, is an instrument developed and produced to simulate cell growth conditions in vitro for mass reproduction and obtain the required normal cells. The large-scale culture technology of adherent growth cells includes roller bottle culture, multilayer culture and microcarrier culture. Among them, roller bottle culture is relatively stable. It can not only serve as a transition stage from small-scale culture to large-scale culture, but also as a way to prepare cells for inoculation in bioreactors. The cell rotary bottle machine is the core part of roller bottle culture. When responding to sudden epidemics, it is crucial to quickly develop corresponding vaccines and quickly produce vaccines on a large scale.
[0003] The traditional cell bottle transfer machine is a machine that drives the cell culture bottle to rotate with a motor. It can be placed in a biological isolation room dedicated to cell culture. The biological isolation room can provide a clean space and an environment such as temperature and humidity for cell growth, so as to carry out high-yield cell culture. However, no matter which type of bottle transfer machine is currently used, the experimenter needs to enter the biological isolation room at a certain interval, take the cell culture bottle from the bottle transfer machine, put it under a microscope for manual observation, and judge whether the cell state can proceed to the next production step through manual experience. On the one hand, frequent entry of personnel into the biological isolation room has a certain risk of contamination. If the cells are cultured with toxic cells, the personnel entering the isolation room also have the risk of infection; on the other hand, taking the cell culture bottle from the bottle transfer machine, observing it, and then putting it back to continue culturing will also affect the growth of cells; thirdly, since it is impossible for manual labor not to change shifts for a long time, the subjective judgment standards of different personnel are inconsistent, which is easy to lead to large differences between production batches, affecting the stability of biological preparations such as vaccines; fourthly, since all the culture bottles of the traditional bottle transfer machine have been in one position, and due to the large space of the isolation room, it is impossible to achieve 100% temperature uniformity at any position, which may cause differences in the yield of different culture bottles for temperature-sensitive cells, thereby affecting the consistency of biological preparations such as vaccines. Utility Model Content
[0004] The main purpose of the utility model is to provide a cell bottle rotating machine with full-automatic monitoring to overcome or improve at least one technical problem of the prior art.
[0005] In order to solve the above technical problems, the utility model adopts the following technical solutions:
[0006] A cell bottle rotating machine with full-automatic monitoring comprises a base, a first column and a second column are installed on the base, a side guard plate is connected between the first column and the second column, a revolving stepper motor is installed on the first column, a rotating stepper motor is installed on the second column, the output end of the revolving stepper motor is connected to a station mounting plate, a plurality of cell culture stations are arranged on the station mounting plate, a tensioning clamp is rotatably installed on the cell culture station, a rotating assembly is connected to the output end of the rotating stepper motor, one end of the tensioning clamp is provided with an opening for mounting a cell culture bottle, and the other end is connected to the rotating assembly via a rotating shaft, a cell detection station is arranged on the base, and a microscopic imaging module is installed on the cell detection station.
[0007] Furthermore, the self-rotating component includes a ring gear, a driven gear and a ring gear mounting plate, the ring gear mounting plate is connected to the output end of the self-rotating stepping motor, the ring gear is fixedly connected to the ring gear mounting plate, a plurality of driven gears are meshed with the inner side of the ring gear, one driven gear corresponds to one tensioning clamp cylinder, and the driven gear is connected to the corresponding tensioning clamp cylinder through the rotating shaft.
[0008] Furthermore, a bottle spinner mainboard is also mounted on the base, and the microscopic imaging module, the orbital stepping motor and the rotational stepping motor are all electrically connected to the bottle spinner mainboard.
[0009] Furthermore, the microscopic imaging module and the bottle transfer machine mainboard are both located between the two side guard plates, the two ends of the side guard plates are respectively fixedly connected to the side walls of the first column and the second column, and the bottom end is connected to the top end of the base.
[0010] Furthermore, a plurality of through grooves are provided on the side wall of the tension clamp cylinder, and the through grooves extend from one end of the opening to the other end, and the length of the through grooves is smaller than the length of the tension clamp cylinder, and the outer diameter of the cell culture bottle is larger than the inner diameter of the opening of the tension clamp cylinder.
[0011] Furthermore, the station mounting plate is a disc structure, and has a plurality of through holes evenly distributed along the circumference, each of the through holes corresponds to a cell culture station, a bearing is installed in the through hole, and the rotating shaft passes through the center hole of the bearing.
[0012] Furthermore, the revolution stepper motor is fixed to the top of the first column through a first mounting bracket, and the rotation stepper motor is fixed to the top of the second column through a second mounting bracket.
[0013] Furthermore, a stop button, a start button and a speed adjustment button are provided on the exposed surface of the first column.
[0014] Compared with the prior art, the utility model has the following beneficial effects:
[0015] The utility model can realize the rotation and revolution of the cell culture bottle, and the revolution stepping motor is used in conjunction with the microscopic imaging module installed on the cell detection station. When the revolution stepping motor drives the cell culture bottle on the station installation plate to rotate to the cell detection station on the bottom plate, the microscopic imaging module can image the cells in the cell culture bottle, thereby realizing automatic monitoring of the cell culture process.
[0016] This solution does not require experimental personnel to enter the biological isolation room at certain intervals, freeing operators from the production process, which is conducive to achieving standardized operating procedures and ensuring product consistency, while also avoiding cell contamination and personnel infection; there is no need to remove the cell culture bottle from the bottle transfer machine and place it under a microscope for manual observation, avoiding the problem of the impact of taking and placing the cell culture bottle back and forth on the cell growth, and also avoiding the problem of differences between production batches caused by inconsistent manual observation and judgment standards. The entire production and culture process is automated, and at the same time, because the spatial position of each cell culture bottle is changing, the difference between bottles caused by uneven temperature in the isolation room is eliminated. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model.
[0018] Figure 2 This is a schematic structural diagram of the utility model from another angle.
[0019] Figure 3 It is a partial structural schematic diagram of the utility model.
[0020] Figure 4 It is a partial structural schematic diagram of the utility model from another angle.
[0021] Figure 5 It is a schematic diagram of the back of the assembly point of the tensioning clamp holding cylinder and the station mounting plate of the utility model.
[0022] Figure 6 It is a schematic diagram of the front part of the assembly of the tensioning clamp holding cylinder and the station mounting plate of the utility model.
[0023] Figure 7 It is a schematic diagram of the structure of the tensioning clamp holding tube of the utility model.
[0024] Figure 8 It is a schematic diagram of the overall structure decomposition of the utility model.
[0025] Fig. 9 This is a schematic diagram of the filling and unloading of the cell culture bottle in the utility model.
[0026] Fig.10 It is a schematic diagram of the self-rotation operation mode of the cell culture bottle in the utility model.
[0027] Fig.11 It is a schematic diagram of the revolution operation mode of the cell culture bottle in the utility model.
[0028] Among them, 1-base, 2-first column, 21-first mounting bracket, 22-stop button, 23-start button, 24-speed adjustment button, 3-second column, 31-second mounting bracket, 4-revolution stepping motor, 41-station mounting plate, 5-rotation stepping motor, 51-gear ring, 52-driven gear, 53-gear ring mounting plate, 6-tensioning clamp cylinder, 61-rotating shaft, 62-bearing, 63-through slot, 7-microscope imaging module, 8-bottle transfer machine mainboard, 9-side guard plate, 10-cell culture bottle. DETAILED DESCRIPTION
[0029] The technical solution of the utility model is further described below through the accompanying drawings and embodiments.
[0030] Combination Figures 1 to 11 The present embodiment provides a cell bottle rotating machine with full-automatic monitoring, including a base 1, on which a first column 2 and a second column 3 are installed, a side guard plate 9 is connected between the first column 2 and the second column 3, a revolving stepper motor 4 is installed on the first column 2, and a rotating stepper motor 5 is installed on the second column 3, the output end of the revolving stepper motor 4 is connected to a station mounting plate 41, and a plurality of cell culture stations are arranged on the station mounting plate 41, a tensioning clamp cylinder 6 is rotatably installed on the cell culture station, the output end of the rotating stepper motor 5 is connected to a rotating assembly, one end of the tensioning clamp cylinder 6 is provided with an opening for installing a cell culture bottle 10, and the other end is connected to the rotating assembly through a rotating shaft 61, a cell detection station is provided on the base 1, and a microscopic imaging module 7 is installed on the cell detection station.
[0031] It should be noted that the existing bottle rotating machine can usually only realize the self-rotation of the cell culture bottle 10 , while the bottle rotating machine of this embodiment can realize both the self-rotation and the revolution of the cell culture bottle 10 by providing the self-rotation stepping motor 5 and the revolution stepping motor 4 .
[0032] By adopting this scheme, a plurality of cell culture stations are arranged on the station mounting plate 41, and the revolving stepper motor 4 can drive the station mounting plate 41 to rotate, that is, the cell culture bottle 10 on each station can be driven to rotate by the revolving stepper motor 4, so that all stations can be moved in space, eliminating the difference between bottles caused by the uneven temperature in the isolation room, and the cell culture bottle 10 can pass through the cell detection station in turn during the rotation (revolution), and then the microscopic imaging module 7 can be used to image the cells in the cell culture bottle 10, so as to realize automatic monitoring of the cell culture process.
[0033] The operator only needs to place the cell culture bottle 10 inoculated with cells into the cell culture station of the rotary bottle machine, and then the entire operation can be performed remotely outside the biological isolation room. For example: when the cell culture bottle 10 inoculated with cells is placed on the cell culture station of the rotary bottle machine, the operator can remotely set the program, set the rotation speed of the cell culture bottle 10, and set the interval for testing the cells. In addition, in order to make the analysis results more accurate, the number of points to be tested for each cell culture bottle 10 can be set, and errors can be eliminated through statistical analysis. At the same time, the results of cell detection and analysis can be automatically determined, and the next command can be triggered based on the determination results. The pain points in production are solved through standardization, intelligence, automation, and reducing manual intervention, avoiding pollution and infection, and improving production efficiency.
[0034] Specifically, it can be set to send an email to the experimenter's mailbox or directly sound and light alarms or automatically reduce the speed or even stop the rotation after a certain result is detected (for example, the cell fusion degree reaches a certain percentage).
[0035] Preferably, the self-rotating assembly includes a ring gear 51, a driven gear 52 and a ring gear mounting plate 53, the ring gear mounting plate 53 is connected to the output end of the self-rotating stepping motor 5, the ring gear 51 is fixedly connected to the ring gear mounting plate 53, a plurality of driven gears 52 are meshed with the inner side of the ring gear 51, one driven gear 52 corresponds to one tensioning clamp cylinder 6, and the driven gear 52 is connected to the corresponding tensioning clamp cylinder 6 through the rotating shaft 61.
[0036] During operation, the rotating stepping motor 5 drives the gear ring mounting plate 53 and the gear ring 51 installed therewith to rotate, and the gear ring 51 drives the driven gear 52 installed at the bottom of each tensioning clamp 6 to rotate. The rotation of the driven gear 52 drives the cell culture bottle 10 to rotate.
[0037] In this embodiment, a bottle spinner mainboard 8 is also installed on the base 1, and the microscopic imaging module 7, the revolution stepping motor 4 and the rotation stepping motor 5 are all electrically connected to the bottle spinner mainboard 8. The bottle spinner mainboard 8 can control the opening and closing of the microscopic imaging module 7, the revolution stepping motor 4 and the rotation stepping motor 5.
[0038] Preferably, the microscopic imaging module 7 and the bottle transfer machine mainboard 8 are both located between the two side guard plates 9, the two ends of the side guard plates 9 are respectively fixedly connected to the side walls of the first column 2 and the second column 3, and the bottom end is connected to the top of the base 1.
[0039] In this embodiment, a plurality of through grooves 63 are provided on the side wall of the tensioning clamp cylinder 6, and the through grooves 63 extend from one end of the opening to the other end. The length of the through grooves 63 is smaller than the length of the tensioning clamp cylinder 6, and the outer diameter of the cell culture bottle 10 is larger than the inner diameter of the opening of the tensioning clamp cylinder 6.
[0040] With this solution, the elastic deformation recovery of the through grooves 63 around the wall of the tension clamping tube 6 is utilized to achieve the effect of clamping and holding the rotating bottle, so that the cell culture bottle 10 can be quickly clamped and taken when it is installed and taken.
[0041] Preferably, the station mounting plate 41 is a disc structure, and has a plurality of through holes evenly distributed along the circumference, each of the through holes corresponding to a cell culture station, a bearing 62 is installed in the through hole, and the rotating shaft 61 passes through the center hole of the bearing 62 .
[0042] Preferably, the revolution stepper motor 4 is fixed to the top of the first column 2 via a first mounting bracket 21 , and the rotation stepper motor 5 is fixed to the top of the second column 3 via a second mounting bracket 31 .
[0043] In this embodiment, the microscopic imaging module 7 completes imaging through an imaging lens and a CMOS / CCD imaging element, and is equipped with a bright field and fluorescent lighting system. It can capture bright field images and various fluorescent images in a timed sequence through program control, and can synthesize dynamic videos of cell changes and analyze various indicators, and give analysis results, so as to provide scientific researchers with objective results without human interference, thereby achieving standardization, intelligence, and automation of the entire experimental process.
[0044] Specifically, during the working process, when the program monitors that the cell culture bottle 10 reaches the observation position of the microscopic imaging module 7, it issues an instruction to stop the bottle spinner (the rotation stepper motor 5 and the revolution stepper motor 4 stop working), and at this time, the microscopic imaging module 7 is used to perform high-definition imaging and recording. The above operations are controlled by the bottle spinner mainboard 8 installed in front of the microscopic imaging module 7. The bottle spinner mainboard 8 can also realize personalized needs such as voice control, Bluetooth, and multi-color fluorescence according to needs.
[0045] The cell bottle transfer machine provided by the utility model can make the entire production and culture process fully automated. At the same time, since the spatial position of each cell culture bottle 10 is changing, the difference between bottles caused by the uneven temperature in the isolation room is eliminated. In this way, the operators are freed from the production process, which is conducive to the realization of standardized operation procedures, and can ensure the consistency of products, while also avoiding cell contamination and personnel infection, solving the pain points in production: standardization, intelligence, automation, and reducing manual intervention, avoiding contamination and infection, and improving production efficiency.
[0046] In another embodiment, a stop button 22, a start button 23 and a speed adjustment button 24 are provided on the exposed surface of the first column 2. By setting the buttons, it can be applied to different working conditions and manual operation can be performed as needed.
[0047] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any slight modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.
Claims
1. A cell bottle transfer machine with fully automatic monitoring, characterized in that: It includes a base, on which a first column and a second column are installed, a side guard plate is connected between the first column and the second column, a revolving stepper motor is installed on the first column, and a rotating stepper motor is installed on the second column. The output end of the revolving stepper motor is connected to a station mounting plate, and a plurality of cell culture stations are arranged on the station mounting plate. A tensioning clamp is rotatably installed on the cell culture station, and a rotating assembly is connected to the output end of the rotating stepper motor. One end of the tensioning clamp is provided with an opening for installing a cell culture bottle, and the other end is connected to the rotating assembly through a rotating shaft. A cell detection station is provided on the base, and a microscopic imaging module is installed on the cell detection station.
2. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 1, characterized in that: The self-rotating component includes a ring gear, a driven gear and a ring gear mounting plate, the ring gear mounting plate is connected to the output end of the self-rotating stepping motor, the ring gear is fixedly connected to the ring gear mounting plate, a plurality of driven gears are meshed with the inner side of the ring gear, one driven gear corresponds to one tensioning clamp cylinder, and the driven gear is connected to the corresponding tensioning clamp cylinder through the rotating shaft.
3. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 2, characterized in that: The base is also provided with a bottle spinner mainboard, and the microscopic imaging module, the orbital stepping motor and the rotational stepping motor are all electrically connected to the bottle spinner mainboard.
4. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 3, characterized in that: The microscopic imaging module and the bottle rotating machine mainboard are both located between the two side guard plates. The two ends of the side guard plates are respectively fixedly connected to the side walls of the first column and the second column, and the bottom end is connected to the top end of the base.
5. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 1, characterized in that: A plurality of through slots are provided on the side wall of the tension clamp cylinder, and the through slots extend from one end of the opening to the other end. The length of the through slots is smaller than the length of the tension clamp cylinder, and the outer diameter of the cell culture bottle is larger than the inner diameter of the opening of the tension clamp cylinder.
6. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 2, characterized in that: The station mounting plate is a disc structure, and has a plurality of through holes evenly distributed along the circumference, each of the through holes corresponds to a cell culture station, a bearing is installed in the through hole, and the rotating shaft passes through the center hole of the bearing.
7. A cell bottle transfer machine with full-automatic monitoring as claimed in claim 1, characterized in that: The revolving stepper motor is fixed to the top of the first column via a first mounting bracket, and the rotating stepper motor is fixed to the top of the second column via a second mounting bracket.
8. The cell bottle rotating machine with full-automatic monitoring as claimed in claim 1, characterized in that: A stop button, a start button and a speed adjustment button are arranged on the exposed surface of the first column.