WB antibody incubation box

Abstract

The utility model discloses a WB antibody incubation box which comprises a box body and a cover body, the rear side of the cover body is hinged to the rear side of the box body, a partition plate is arranged at the bottom in the box body in the left-right direction and divides an inner cavity of the box body into a front area and a rear area, and meanwhile, a WB film is also divided into a front part and a rear part by the partition plate and located in the front area and the rear area of the box body respectively. When an experiment needs to be carried out, the WB film is laid in the box body, and the partition plate supports the middle of the bottom face of the WB film; the device further comprises a film pressing plate, the film pressing plate covers the WB film, a groove is formed in the position, corresponding to the partition plate, of the bottom of the film pressing plate, and a groove opening faces downwards. By adopting the nested design of the film pressing plate and the partition plate, protein strips with different sizes on the WB film can be separated in different areas, and antibodies are added to two sides and cannot be mixed with each other; compared with the traditional method without adopting the film pressing plate, the space between the cover pressing plate and the bottom of the box is reduced as much as possible while the full contact between the WB film and the antibody is ensured, so that the dosage of the antibody is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to a WB antibody incubation box. Background Art

[0002] Western blot (WB) is one of the most commonly used and widely used experimental methods for detecting protein expression in life science related experiments. Its principle is based on the difference in protein molecular size, and the proteins in the sample are separated by vertical gel electrophoresis. These proteins are then transferred to a WB membrane (PVDF membrane or NC membrane), and then specific antibodies are used to bind to the target protein to form a protein-antibody complex. Finally, these complexes are detected by adding dyes or luminescent agents to determine whether the target protein exists and its expression abundance. WB is widely used in basic research, clinical diagnosis, and drug development. In basic research, it is often used to study the expression level, subtype and modification state of proteins, as well as protein interactions. In clinical diagnosis, WB can be used to detect the presence of specific proteins, such as tumor markers or the diagnosis of infectious diseases. In addition, WB can also be used in drug development to evaluate the effects of drugs on specific proteins and the targeting of drugs. Especially in basic research, WB is the main means of detecting the expression of target proteins. An important step in the WB experiment is the incubation of antibodies, including the incubation of primary and secondary antibodies with the target protein adsorbed on the WB membrane. At present, the common practice of antibody incubation during WB experiments is to cut the membrane containing the target protein according to the predicted protein molecular weight with reference to the protein marker, and then put the membranes containing different target proteins into different incubation boxes for antibody incubation respectively. For example, a WB antibody incubation box for preventing accidental inversion disclosed in Chinese patent CN 213148946 U includes an incubation box, a cover, a partition and a slide plate, wherein the incubation box is hinged with the cover, and a plurality of partitions are arranged inside the incubation box to separate the incubation box into a plurality of areas. However, the problem with this incubation box is that the amount of antibody required is relatively large. At present, there is also a method of not cutting the WB membrane and mixing the antibodies of the multiple target proteins to be detected and incubating them simultaneously, but this method may have mutual influences between different antibodies, resulting in the final effect not being very ideal, especially for proteins with low expression levels, the target band may not be detected. Utility Model Content

[0003] The utility model aims to provide a WB antibody incubation box to solve the problems in the prior art that a relatively large amount of antibodies is required and mixed incubation of different antibodies affects each other.

[0004] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0005] A WB antibody incubation box comprises a box body and a cover body, wherein the rear side of the cover body is hinged to the rear side of the box body; a partition is provided at the bottom of the box body along the left-right direction, the partition divides the inner cavity of the box body into two front and rear areas, when the WB film is laid, the partition supports the middle part of the bottom surface of the WB film, and at the same time, the WB film is also divided into two front and rear parts by the partition, and is respectively located in the front and rear areas of the box body; and a film pressing plate is also included, the film pressing plate covers the top of the WB film, and a groove is provided at the bottom of the film pressing plate corresponding to the partition, with the groove facing downward.

[0006] Furthermore, a pressure strip is vertically provided on the bottom surface of the cover body, and the pressure strip corresponds to the partition.

[0007] Furthermore, it also includes an upper film pressing strip and a lower film pressing strip, wherein the lower film pressing strip is arranged in parallel in the left-right direction inside the box body and is located at the front and rear sides of the partition; the upper film pressing strip corresponds to the lower film pressing strip and is arranged at the bottom of the film pressing plate. The lengths of the upper film pressing strip and the lower film pressing strip are shorter than the film pressing plate, so that there is a certain space between the upper film pressing strip and the lower film pressing strip and the side wall of the box body, which serves as a channel for the antibody to enter.

[0008] Furthermore, it also includes auxiliary pressure strips, which are arranged at the bottom of the film pressing plate and located on both sides of the edge of the groove notch.

[0009] Furthermore, the length of the auxiliary pressure strip extending downward is consistent with the length of the upper film pressing strip extending downward.

[0010] Furthermore, it also includes a buckle, which includes a hook and an arc-shaped card. The hook is arranged on the front side of the cover body, and the arc-shaped card is arranged on the front side of the box body. The hook and the arc-shaped card are clamped to each other.

[0011] Furthermore, the film pressing plate is provided with exhaust holes.

[0012] Furthermore, a gap is left between the front and rear sides of the film pressing plate and the inner wall of the box body as a sample adding groove.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. The utility model adopts a nested design of a film pressing plate and a partition, which can separate protein bands of different sizes on the WB membrane into different areas, and antibodies added on both sides will not mix with each other; compared with the traditional method without a film pressing plate, while ensuring full contact between the WB membrane and the antibody, the space between the cover pressing plate and the bottom of the box is reduced as much as possible, thereby reducing the amount of antibody used.

[0015] 2. The arrangement of the upper and lower membrane pressing strips of the utility model can fully unfold the WB membrane and maintain the space for the membrane to contact the antibody. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1It is a schematic diagram of the three-dimensional structure of the utility model when the cover is opened;

[0017] Figure 2 It is a schematic diagram of the structure of the utility model when the cover is opened from above;

[0018] Figure 3 It is a schematic diagram of the three-dimensional structure of the utility model when the cover is closed;

[0019] Figure 4 It is a schematic diagram of the structure of the utility model when the cover is closed from above;

[0020] Figure 5 It is a cross-sectional schematic diagram of the structure of the utility model in the AA direction when the cover body is closed;

[0021] Figure 6 It is a cross-sectional schematic diagram of the structure of the utility model when the cover is closed, viewed from the top in the direction of BB;

[0022] The serial numbers and names in the figure are as follows:

[0023] 1-box body; 2-cover body; 3-WB film; 4-partition; 5-film pressing plate; 6-groove; 7-pressing plate strip; 8-upper film pressing strip; 9-lower film pressing strip; 10-auxiliary pressing strip; 11-hook; 12-arc card; 13-exhaust hole; 14-sample adding slot. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. It should be understood that the directions of "up", "down", "left", "right", "front" and "back" mentioned in the following embodiments of the utility model are all based on the position of the main view.

[0025] Example

[0026] A WB antibody incubation kit, such as Figures 1 to 4As shown, it includes a box body 1 and a cover body 2, the rear side of the cover body 2 is hinged to the rear side of the box body 1, so that the cover body 2 can be rotated to open or close; a partition 4 is provided on the central axis of the left and right directions of the bottom of the box body 1, and the partition 4 divides the inner cavity of the box body 1 into two front and rear areas. When an experiment is required, a WB membrane 3 is laid in the box body 1, and the partition 4 supports the middle part of the bottom surface of the WB membrane 3. At the same time, the WB membrane is also divided into two front and rear parts by the partition, and is respectively located in the front and rear areas of the box body, so that the two target protein bands on the WB membrane 3 are respectively placed in the two areas, and the partition 4 ensures that the antibodies in the two areas do not leak from each other; the WB membrane 3 covers the top of the partition 4; it also includes a film pressing plate 5, the projection area of ​​the film pressing plate 5 is equivalent to the inner area of ​​the box body 1, and it covers the top of the WB membrane 3. A groove 6 is provided on the film pressing plate 5 corresponding to the partition 4, and the notch is downward. The groove 6 is covered on the partition 4, and the film pressing plate 5 and the partition 4 fix the WB membrane 3 together.

[0027] In this embodiment, a pressure strip 7 is vertically provided on the bottom surface of the cover body 2, and the pressure strip 7 corresponds to the partition 4. After the cover body 2 is closed, the pressure strip 7 applies pressure to the film pressing plate 5 to fix the film pressing plate 5 and the WB film on the partition 4.

[0028] In this embodiment, it also includes an upper film pressing strip 8 and a lower film pressing strip 9. The lower film pressing strip 9 is arranged in parallel in the left-right direction inside the box body 1, and is located on the front and rear sides of the partition 4; the upper film pressing strip 8 corresponds to the lower film pressing strip 9 and is arranged at the bottom of the film pressing plate 5, and the WB film 3 and the film pressing plate 5 are both located between the upper film pressing strip 8 and the lower film pressing strip 9, ensuring that the WB film 3 is in the middle position at the bottom of the box body 1, and the upper and lower surfaces of the WB film 3 can fully contact the antibody. The length of the upper film pressing strip 8 and the lower film pressing strip 9 are both shorter than the film pressing plate 5, so that there is a certain space with the side wall of the box body 1 as a channel for the antibody to enter. A gap is left between the front and rear sides of the film pressing plate 5 and the inner wall of the box body 1 as a sample loading slot 14.

[0029] In this embodiment, a pressure-assisting strip 10 is further included. The pressure-assisting strip 10 is arranged at the bottom of the film pressing plate 5 and located on both sides of the edge of the notch of the groove 6 .

[0030] In this embodiment, the downward extension length of the auxiliary pressure strip 10 is consistent with the downward extension length of the upper film pressing strip 8 , so that the WB film 3 can be fully unfolded and laid flat in the areas on both sides of the partition 4 .

[0031] In this embodiment, a buckle is also included, which includes a hook 11 and an arc-shaped card 12. The hook 11 is arranged on the front side of the cover body 2, and the arc-shaped card 12 is arranged on the front side of the box body 1. The hook 11 and the arc-shaped card 12 are clamped together, so that the pressure plate strip 7 can provide a stable downward pressure.

[0032] In this embodiment, the laminating plate 5 is made of a transparent PE material and is provided with an exhaust hole 13 for removing air bubbles between the laminating plate 5 and the WB film 3 .

[0033] The method of using the utility model is:

[0034] (1) After the WB experiment transfer is completed and the steps of sealing and washing the membrane are completed, the WB membrane 3 is covered on the bottom of the incubation box;

[0035] (2) Press the film pressing plate 5 onto the WB film 3 so that the groove 6 of the film pressing plate 5 fits into the partition plate 4;

[0036] (3) Add 200 μL of antibody A and antibody B working solutions to the areas on both sides of the partition 4 through the sample adding groove 14, so that the antibodies fill the space between the pressing plate 5 and the bottom of the box body 1; if there are bubbles, use a pipette tip or tweezers to gently touch the pressing plate 5 to discharge the bubbles from the exhaust hole 13 or the sample adding groove 14;

[0037] (4) Cover the cover 2, fasten the cover 2 with a buckle, and press the pressing plate 5, WB membrane 3 and partition 4 with the pressing plate strip 7 to prevent the antibodies in the area on both sides of the partition 4 from leaking and mixing; then place the incubation box on a shaker and gently shake or leave it still, and incubate according to the temperature and time required by the experiment;

[0038] (5) After the antibody incubation is completed, use a pipette to absorb the antibody working solution through the sample loading slot 14 for recovery and reuse; then add the membrane cleaning solution, clean it according to the conventional experimental steps, and proceed to the next step of the experiment.

[0039] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A WB antibody incubation box, comprising a box body (1) and a cover body (2), wherein the rear side of the cover body (2) is hinged to the rear side of the box body (1), characterized in that: The box body (1) is provided with a partition (4) along the left-right direction at the bottom of the box body (1), and the partition (4) divides the inner cavity of the box body (1) into two front and rear areas. When the WB film (3) is laid, the partition (4) supports the middle part of the bottom surface of the WB film (3); and also includes a film pressing plate (5), which covers the top of the WB film (3), and a groove (6) is provided at the bottom of the film pressing plate (5) corresponding to the partition (4), with the groove facing downward.

2. The WB antibody incubation box according to claim 1, characterized in that: A pressure strip (7) is vertically arranged on the bottom surface of the cover body (2), and the pressure strip (7) corresponds to the partition (4).

3. The WB antibody incubation box according to claim 1, characterized in that: It also includes an upper film pressing strip (8) and a lower film pressing strip (9), wherein the lower film pressing strip (9) is arranged in parallel in the left-right direction inside the box body (1) and is located on the front and rear sides of the partition (4); the upper film pressing strip (8) is arranged at the bottom of the film pressing plate (5) corresponding to the lower film pressing strip (9).

4. The WB antibody incubation box according to claim 1, characterized in that: It also includes a pressure-assisting strip (10), which is arranged at the bottom of the film pressing plate (5) and located on both sides of the notch edge of the groove (6).

5. The WB antibody incubation box according to claim 4, characterized in that: The length of the auxiliary pressure strip (10) extending downward is consistent with the length of the upper film pressing strip (8) extending downward.

6. The WB antibody incubation box according to claim 1, characterized in that: The invention also comprises a buckle, wherein the buckle comprises a hook (11) and an arc-shaped card (12), wherein the hook (11) is arranged on the front side of the cover body (2), and the arc-shaped card (12) is arranged on the front side of the box body (1), and the hook (11) and the arc-shaped card (12) are clamped to each other.

7. The WB antibody incubation box according to claim 1, characterized in that: The film pressing plate (5) is provided with an exhaust hole (13).

8. The WB antibody incubation box according to claim 1, characterized in that: A gap is left between the front and rear sides of the film pressing plate (5) and the inner wall of the box body (1) as a sample adding groove (14).

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