Lightweight platelet function detection device

Abstract

The utility model discloses a lightweight platelet function detection device. The lightweight platelet function detection device comprises a detection module and a sample processing module, the sample treatment module comprises a multifunctional liquid suction needle for sucking liquid, a driving device and a dilution module; the multifunctional liquid suction needle is connected with the dilution module through the liquid path pipe, and the driving device controls the multifunctional liquid suction needle to move. The detection module comprises one or more sample cup position devices for placing sample cups, and one or more built-in reagent positions are arranged in the sample cup position devices. All the sample cup positions and the built-in reagent positions are arranged in a movement track area of the multifunctional liquid suction needle. According to the design, the structure and the flow of the platelet function detector adopting the original continuous counting method are simplified, and electromagnetic valves required by the device are reduced by 30%, so that the cost of the device is reduced, and the failure rate is correspondingly reduced.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a lightweight platelet function testing device. Background Technology

[0002] This section provides only background information relevant to this disclosure and is not necessarily prior art.

[0003] Platelet function testing plays a vital role in the early warning and prevention of thrombotic diseases, and in guiding the safe, effective, and precise application of antiplatelet drugs in the treatment of thrombotic diseases. In addition, it also plays a vital role in other clinical platelet function-related tests and related drug research.

[0004] The ideal sample for platelet function testing is citrate-anticoagulated whole blood. However, platelet function in citrate-anticoagulated whole blood is easily affected by environmental factors such as temperature, vibration, and time. Therefore, rapid platelet function testing is essential in clinical medical services and related research. For whole blood testing, it is also crucial to maintain the platelet count and volume (including platelets) in the blood sample at all times to obtain accurate results.

[0005] Platelet function testing has a history of over 60 years. To explore ideal instruments and methods for platelet function testing, scholars both domestically and internationally have developed various platelet function testing devices based on different principles and methods. Among them, the continuous counting method for platelet function testing yields relatively good results. However, this instrument requires more than one diluent to operate, and its sample mixing device is insufficient for mixing viscous blood samples with reagents, leading to incomplete reactions in some samples. To simplify the complex structure of the original continuous counting platelet function instrument, improve the thorough mixing of blood samples and reagents, and better enhance the quality of platelet function test results, the original continuous counting platelet function instrument was improved. However, the original design still has a complex structure, high cost, and insufficient mixing of blood samples and reagents. Summary of the Invention

[0006] To address the shortcomings of the prior art, this invention discloses a lightweight platelet function detection device.

[0007] A lightweight platelet function detection device includes a detection module, a sample processing module, and a detection module.

[0008] The sample processing module includes a multifunctional aspiration needle for drawing liquid, a driving device, and a dilution module for diluting the sample. The multifunctional aspiration needle is connected to the dilution module via a liquid circuit, and the driving device controls the movement of the multifunctional aspiration needle. The multifunctional aspiration needle enables quantitative aspiration and dispensing of reagents, and simultaneously has the function of quantitatively drawing and diluting samples and dispensing them according to the detection requirements. The multifunctional aspiration needle, in conjunction with the dilution module, quantitatively adds the diluent to the test cup. The dilution module is connected to a dilution container via tubing.

[0009] The detection module is used to detect the cellular components and plasma components of blood samples. The detection module includes a sample cup position for placing sample cups and a built-in reagent position for placing test reagents. The built-in reagent positions are all located on the movement trajectory of the multifunctional aspiration needle.

[0010] The multi-functional aspiration needle has the ability to move in more than one dimension. It can be inserted into the sample cup to draw up the mixed blood sample and distribute it to the dilution cup. A certain amount of diluent is added to the dilution cup. After the blood sample is diluted and mixed in the dilution cup, the multi-functional aspiration needle draws up a quantitative amount of diluted blood sample from the dilution cup and distributes it to the test cup for testing. The device is also equipped with a diluent tank, which can be external or internal. The sample cups that come with the device can be independent single cups or multiple single cups with the same structure and shape combined into a joint cup.

[0011] Furthermore, the built-in reagent positions include a built-in reagent position for placing a dilution cup, a detection position for placing a test cup, and a detection position for placing a reagent bottle. The reagent bottle contains an agglutinating agent. The dilution cup and the sample cup are each equipped with a stirring device. The lower part of the dilution cup and the test cup are equipped with a drain port. The dilution cup and the test cup are connected to the waste liquid treatment module through the drain port.

[0012] The waste liquid treatment module includes a third liquid flow control valve and a first discharge pump. The dilution cup and the detection cup are both connected to the third liquid flow control valve through a waste liquid discharge pipe. The third liquid flow control valve is connected to the first discharge pump through a waste liquid discharge collection pipe.

[0013] Furthermore, in some embodiments, the stirring device includes a magnetic stirring device and a magnetic stir bar. The magnetic stir bar is disposed inside the test cup, and the magnetic stirring device is disposed at the bottom of the test cup. The magnetic stirring device stirs the sample by driving the magnetic stir bar to move through magnetic force.

[0014] Both the dilution cup and the test cup have radial shallow grooves at their bottoms, which are adapted to the shape of the magnetic stir bar. After stirring stops, the magnetic stir bar falls into the radial shallow grooves and remains in a stable, stationary state when not stirring. This avoids interference with the test results caused by the movement of the stir bar during testing.

[0015] Furthermore, in some embodiments, the stirring device is an aeration mixing needle disposed in the dilution cup and the test cup and an air supply pump connected to the aeration mixing needle. The exhaust port of the aeration mixing needle is located in the dilution cup and the test cup, respectively. The air supply pump provides agitating gas to the aeration mixing needle to stir and mix the liquid in the dilution cup and the test cup.

[0016] Furthermore, the dilution module includes a diluent and a diluent container for holding the diluent; the multifunctional aspiration needle, the first liquid path tube, the diluent, the second liquid path tube, and the diluent container are connected in sequence. The first liquid path tube is equipped with a first flow control valve, and the second liquid path tube is equipped with a second flow control valve. This system performs multiple functions, including blood sample aspiration, reagent aspiration and dispensing, and direct quantitative dispensing of diluent into dilution cups and test cups, as well as adding platelet function test reagents to sample cups.

[0017] When diluent needs to be added to the test cup via the multi-functional aspiration needle, the first flow control valve closes and the second flow control valve opens. The piston of the diluent is pulled back, creating a negative pressure in the second liquid passage, which draws the diluent from the diluent tank into the diluent chamber. Subsequently, the second flow control valve closes and the first flow control valve opens, pushing the piston of the diluent forward. The diluent drawn into the diluent chamber is then distributed to the test cup via the first liquid passage to the multi-functional aspiration needle.

[0018] Furthermore, both the test cup and the reagent bottle are equipped with automatic opening and closing caps. These caps open and close in conjunction with the movement of the multi-functional aspiration needle. When the multi-functional aspiration needle needs to penetrate the reagent bottle to draw reagents, or when a sample or reagent needs to be added to the test cup, the automatic opening and closing caps open automatically. Conversely, when the multi-functional aspiration needle has completed drawing and mixing a sample from the test cup, adding reagent / diluent to the test cup, or completing reagent drawing, the automatic opening and closing caps close automatically to prevent the liquid drawn by the multi-functional aspiration needle from dripping into the lower test cup or reagent bottle. These automatic opening and closing caps also prevent reagents carried by the multi-functional aspiration needle and related devices from dripping into the test cup and reagent bottle.

[0019] The material of the automatic opening and closing cover is resistant to external electrical signal interference. This resistant material can be aluminum foil shielding. During the detection process, this reduces interference from external electromagnetic signals, improving the quality of the detection results.

[0020] Furthermore, the platelet function testing device is equipped with a special cleaning agent.

[0021] In some embodiments, the lightweight platelet function testing device performs the test without pre-adding platelet aggregation inducers or platelet function inhibitors to the sample. The workflow includes the following steps:

[0022] Step 1, Pre-processing: Add the loaded blood sample and magnetic stir bar to the sample cup, place the sample cup in the sample cup position, and start the device. The device will mix the sample in the sample cup using the stirring device.

[0023] The third liquid flow control valve at the bottom of the dilution cup and the test cup is opened, and the first discharge pump works to discharge the diluent originally stored in the dilution cup and the test cup. The multi-functional aspiration needle and the diluent work together to add a certain amount of diluent to the dilution cup and the test cup respectively, and the dilution cup and the test cup are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0024] The multi-functional aspiration needle, in conjunction with the diluent, adds a certain amount of diluent to the release cup and the test cup respectively, and the diluent added to both cups remains in the cup.

[0025] Step 2: Dilute the sample. The multi-functional aspiration needle is moved to one of the sample cups. The multi-functional aspiration needle and diluent work together to draw a certain amount of blood sample and transfer it to the dilution cup. A certain amount of diluent is added to the dilution cup, and the blood sample in the dilution cup is mixed by the stirring device.

[0026] Step 3, raw testing: A certain amount of diluted blood sample is drawn from the dilution cup by a multi-functional aspiration needle and transferred to the test cup. A certain amount of diluent is added to the test cup, and the blood sample in the test cup is mixed by a stirring device. The test cup is then tested by a testing device to obtain information data on the platelets in the original state of the blood sample.

[0027] Step 4, waste liquid treatment: the drain channel at the bottom of the dilution cup and the test cup is opened, and the waste liquid in the dilution cup and the test cup is discharged through the connecting pipe and the first drain pump.

[0028] Step 5: Cleaning device. A certain amount of diluent is added to the dilution cup and the test cup respectively through the multi-functional aspiration needle and diluent. The dilution cup and the test cup are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0029] Step 6: Add a certain amount of diluent to the release cup and the test cup respectively using a multi-functional aspiration needle and a diluent.

[0030] Step 7, reaction detection: A certain amount of agglutinant is drawn from the reagent bottle using a multi-functional aspiration needle and added to the sample cup after the original blood sample test is completed. The blood sample and reagent in the sample cup are stirred and mixed using a stirring device. After sufficient reaction time, the multi-functional aspiration needle is used to draw the reacted blood sample from the sample cup and the test is performed according to steps 1-5 above to obtain information such as the number and volume of platelets in the blood sample after the agglutinant treatment.

[0031] Step 8, data processing: The device compares the changes in platelet count and volume of the blood sample before and after the addition of the agglutinant according to the above process to obtain the platelet function level test result of the blood sample.

[0032] Step 9: Repeat the test. The device repeatedly performs platelet function tests on different blood samples according to steps 1-8 above.

[0033] When a platelet function testing device tests more than one blood sample at the same time, the timing of platelet count testing of different original blood samples and the order of adding agglutinating agents can be optimized to achieve optimized testing of more than one sample, or to complete the testing of different samples one by one according to the above steps to obtain different platelet receptor function test results of different samples, or the same sample with different results.

[0034] In some embodiments, the platelet function testing device may pre-divide the same blood sample into multiple portions, with one portion containing a strong platelet function inhibitor such as EDTA, and the remaining portions containing different platelet receptor agglutinators. Under these conditions, the device does not have a built-in reagent compartment for the agglutinator, but rather one or more sample cups. Each time, multiple sample cups are loaded with the same blood sample, but each cup contains different reagents pre-added, and each cup is pre-loaded with a magnetic stir bar. The device directly aspirates and tests the blood sample from each cup, calculating the platelet function of each sample based on the number or volume of platelets obtained from the tests. Under these conditions, the device's workflow is as follows:

[0035] Step 1, Preparatory work: Place at least two sample cups containing equal amounts of the same blood sample in the sample cup position, and add a magnetic stir bar to each sample cup. Add different reagents to each sample cup for treatment. Add a platelet function inhibitor to one sample cup, and add a platelet receptor function agglutinant to the other blood samples. Use a stirring device to mix each sample.

[0036] The third liquid flow control valve at the bottom of the dilution cup and the test cup is opened, and the first drain pump works to discharge the diluent originally stored in the dilution cup and the test cup; the multi-functional aspiration needle and the diluent work together to add a certain amount of diluent to the dilution cup and the test cup respectively, and the dilution cup and the test cup are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0037] The multi-functional aspiration needle, in conjunction with the diluent, adds a certain amount of diluent to the release cup and the test cup respectively, and both cups retain the diluent.

[0038] Step 2: Dilute the sample. The multifunctional aspiration needle is moved into a sample cup. The multifunctional aspiration needle and diluent work together to draw a certain amount of blood sample and transfer it into the dilution cup. A certain amount of diluent is added to the dilution cup, and the blood sample in the dilution cup is mixed by the stirring device.

[0039] Step 3, Sample Detection: A certain amount of diluted blood sample is drawn from the dilution cup by a multi-functional aspiration needle and transferred to the detection cup. A certain amount of diluent is added to the detection cup, and the blood sample in the detection cup is diluted and mixed again by a stirring device. The detection cup is then tested by a detection device to obtain the platelet count and volume information of the blood sample in the blood sample cup.

[0040] Step 4, waste liquid treatment: the drain channel at the bottom of the dilution cup and the test cup is opened, and the waste liquid in the dilution cup and the test cup is discharged through the connecting pipe and the first drain pump.

[0041] Step 5: Cleaning device. A certain amount of diluent is added to the dilution cup (6) and the test cup (7) respectively by using the multi-functional aspiration needle (2) and the diluent (11). The dilution cup (6) and the test cup (7) are stirred and rinsed by the stirring device. After rinsing, the diluted solution in the dilution cup (6) and the test cup (7) is discharged.

[0042] Step 6: Using a multi-functional aspiration needle and diluent, add a certain amount of diluent to the release cup and the test cup respectively. At this time, the diluent added to both cups remains in the cup.

[0043] Step 7: Repeat steps 2-6 to test the blood samples in the remaining sample cups to obtain information on the platelet status of the same blood samples after being treated with different reagents.

[0044] Step 8: Based on the differences in test results between the same blood samples treated with platelet aggregation inducers and platelet function inhibitors, the platelet function level of the blood sample is compared to obtain the test results. This is based on the fact that citrate-anticoagulated blood samples have platelet activation function. After adding a platelet aggregation inducer, platelet activation and aggregation decrease, and the degree of reduction is related to the platelet function level. Conversely, when a strong anticoagulant, such as EDTA, is added to a citrate-anticoagulated blood sample, platelet function is completely inhibited. The number of platelets obtained from the EDTA-treated blood sample is taken as the original platelet count of that blood sample. By comparing the platelet counts of the same blood sample treated with strong platelet function inhibitors and those treated with aggregation inducers, the platelet function test results for that blood sample can be obtained.

[0045] Step 9: Repeat steps 1-8 to test platelet function in different blood samples. Blood samples tested according to the above steps should be tested within 30 minutes of adding the reagents.

[0046] Furthermore, the platelet function testing device has at least one built-in reagent position for cleaning agent, which is used for cleaning when cleaning conditions are met. These cleaning conditions include before daily startup, when the device fails to perform testing for a specified time after testing, and other set conditions. The cleaning steps include:

[0047] Step S1: The multi-functional aspiration needle draws a certain amount of cleaning agent from the cleaning agent bottle and discharges it into the dilution cup and the test cup respectively. The cleaning agent soaks in the dilution cup and the test cup for a certain period of time.

[0048] Step S2: Stir the cleaning agent in the dilution cup and the test cup using a stirring device.

[0049] In step S3, the third liquid flow control valve at the bottom of the dilution cup and the test cup is opened, and the first drain pump is activated to discharge the cleaning agent from the dilution cup and the test cup.

[0050] Step S4: Repeat steps S1-S3 until the cleaning requirements are met.

[0051] Furthermore, the sample cup and built-in reagent compartments of the platelet function testing device are partially or entirely equipped with temperature control devices. The sample cup compartment is generally set to a temperature of 24-35℃, and the temperature of the built-in reagent compartment is generally set to a range of 5-20℃. The temperature of the diluent injection pipeline can be set to 15-25℃.

[0052] Furthermore, the sample cup of the platelet function testing device is formed by combining at least two cups of the same structure to form a single row of combined sample cups, with each cup being independent and not connected to the others; the height of the combined sample cup is 12-55mm, and the inner diameter of each cup is 5-15mm.

[0053] The principles of the two workflows for calculating platelet function from the platelet count results of a blood sample using a platelet function testing device are as follows:

[0054] Comparison of platelet count changes before and after the addition of the agglutinin:

[0055] In the original blood sample without the addition of a platelet aggregation inducer, the platelet count is relatively high. However, in the blood sample with high platelet function after the addition of the inducer, platelets will aggregate to a greater extent, resulting in a decrease in the platelet count. Conversely, in the blood sample with low platelet function, platelet aggregation is less pronounced, and the decrease in platelet count is smaller. By comparing the platelet counts obtained from the blood samples before and after the addition of the inducer using a comparative device, the degree of platelet reduction can be calculated, thus determining the platelet function of the blood sample.

[0056] Comparison of platelet counts obtained from the same blood sample tested with different reagents:

[0057] This detection method involves loading the same blood sample into different sample cups. One cup contains a platelet function inhibitor or a strong anticoagulant such as EDTA, while the other cups contain platelet aggregation inducers such as arachidonic acid or adenosine diphosphate. Because the addition of a strong anticoagulant inactivates platelets and prevents aggregation, while the addition of a platelet aggregation inducer results in a higher degree of platelet aggregation in samples with high platelet function, thus significantly reducing the platelet count, the platelet count decreases only slightly in samples with low platelet function. Therefore, by comparing the changes in platelet count after adding different types of reagents to the blood sample, the platelet function test results for that blood sample can be obtained.

[0058] Beneficial effects:

[0059] The present invention provides a lightweight platelet function testing device and its workflow, which greatly simplifies the original platelet function testing instrument based on the continuous counting method. The device reduces the number of diluents and the number of solenoid valves used is reduced by up to 40%, thus reducing the device cost and the failure rate accordingly.

[0060] At the same time, a corresponding device workflow was designed based on the simplified device structure, enabling the simplified device to complete platelet function testing.

[0061] A platelet function testing device with a simple structure will significantly reduce costs, which will facilitate the popularization of platelet function testing services in primary healthcare institutions.

[0062] Previous platelet function devices required the use of diluent to clean the sampling needle. However, the platelet function detection device designed in this paper uses the diluent for cleaning the needle, which greatly reduces the reagent consumption of the device and is more conducive to reducing the operating cost of the device. Attached Figure Description

[0063] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0064] Figure 1 This is a schematic diagram of the main structural relationships of the device in this design.

[0065] Figure 2 This is a schematic diagram of the relevant structure of the device when equipped with two diluters. Detailed Implementation

[0066] The reference numerals in the attached drawings are explained as follows: 101-First sample cup; 102-Second sample cup; 2-Multifunctional aspiration needle; 3-Fixed drive device; 4-Moving slide rail; 5-First liquid passage tube; 6-Dilution cup; 7-Detection cup; 801-First reagent bottle; 802-Second reagent bottle; 9-Second liquid passage tube; 10-Dilution tank; 11-Diluter; 12-Detection device; 13-First drain pump; 14-Magnetic stirring device; 15-Magnetic stir bar; 16-Waste liquid discharge tube; 17-Waste liquid discharge collection tube; 18-First liquid flow control valve; 19-Second liquid flow control valve; 20-Third liquid flow control valve; 21-Second drain pump; 22-Fourth liquid flow control valve; 23-Second diluent; 24-Fifth liquid flow control valve; 25-Needle surface cleaning device; 26-Sixth liquid flow control valve.

[0067] like Figure 1 As shown, a lightweight platelet function detection device includes a detection module, a sample processing module, and a detection module. The sample processing module includes a multifunctional aspiration needle 2 for drawing liquid, a driving device, and a dilution module for diluting the sample. The multifunctional aspiration needle 2 is connected to the dilution module via a liquid circuit, and the driving device controls the movement of the multifunctional aspiration needle 2. The multifunctional aspiration needle 2 enables quantitative aspiration and dispensing of reagents, and simultaneously has the function of quantitatively drawing and diluting samples and dispensing them according to detection needs. The multifunctional aspiration needle 2, in conjunction with the dilution module, quantitatively adds the diluent to the detection cup.

[0068] The detection module is used to detect the cellular components and plasma components of blood samples. The detection module includes a sample cup position for placing sample cups and a built-in reagent position for placing test reagents. The built-in reagent positions are all located on the movement trajectory of the multifunctional aspiration needle 2.

[0069] Furthermore, the built-in reagent positions include a built-in reagent position for placing the dilution cup 6, a detection position for placing the test cup 7, and a detection position for placing the reagent bottle. The reagent bottle contains an agglutinating agent. The test cup 7 is equipped with a platelet detection device and a stirring device for mixing the diluted sample solution in the cup. The dilution cup 6 and the sample cup are each equipped with a stirring device. The lower part of the dilution cup 6 and the test cup 7 is provided with a drain port. The dilution cup 6 and the test cup 7 are connected to the waste liquid treatment module through the drain port.

[0070] The waste liquid treatment module includes a third liquid flow control valve 20 and a first drain pump 13. The dilution cup 6 and the detection cup 7 are both connected to the third liquid flow control valve 20 through the waste liquid discharge pipe 16. The third liquid flow control valve 20 is connected to the first drain pump 13 through the waste liquid discharge collection pipe 17.

[0071] Furthermore, in some embodiments, the stirring device includes a magnetic stirring device 14 and a magnetic stir bar 15. The magnetic stir bar 15 is disposed inside the test cup, and the magnetic stirring device 14 is disposed at the bottom outside the test cup. The magnetic stirring device 14 stirs the sample by driving the magnetic stir bar 15 to move through magnetic force.

[0072] The bottom of the dilution cup 6 and the test cup 7 are respectively provided with radial shallow grooves. The radial shallow grooves are adapted to the shape of the magnetic stir bar 15. After stirring stops, the magnetic stir bar 15 falls into the radial shallow grooves. When not stirring, the magnetic stir bar 15 is in a stable static state.

[0073] Furthermore, in some embodiments, the stirring device is an aeration mixing needle disposed in the dilution cup 6 and the detection cup 7 and an air supply pump connected to the aeration mixing needle. The exhaust port of the aeration mixing needle is located in the dilution cup 6 and the detection cup 7, respectively. The air supply pump provides agitating gas to the aeration mixing needle to stir and mix the liquid in the dilution cup 6 and the detection cup 7.

[0074] Furthermore, the dilution module includes a diluent 11 and a diluent tank 10 for holding the diluent; the multifunctional suction needle 2, the first liquid passage tube 5, the diluent 11, the second liquid passage tube 9 and the diluent tank 10 are connected in sequence, the first liquid passage tube 5 is provided with a first liquid flow control valve 18, and the second liquid passage tube 9 is provided with a second liquid flow control valve 19.

[0075] When diluent needs to be added to the test cup through the multi-functional aspiration needle 2, the first liquid flow control valve 18 is closed and the second liquid flow control valve 19 is opened. The piston of the diluent 11 is pulled back, creating a negative pressure in the second liquid passage 9, which causes the diluent in the diluent tank 10 to be drawn into the diluent chamber. Subsequently, the second liquid flow control valve 19 is closed and the first liquid flow control valve 18 is opened. The piston of the diluent 11 is pushed forward, and the diluent drawn into the diluent chamber of the diluent 11 is then sent to the multi-functional aspiration needle 2 through the first liquid passage 5 and distributed into the test cup.

[0076] Example 1:

[0077] The platelet function testing device calculates platelet function by comparing changes in platelet counts before and after the addition of an agglutinant to the blood sample. The workflow includes the following steps:

[0078] Step 1, Pre-testing: Add the loaded blood sample to the sample cup and mix the sample using a stirring device.

[0079] The third liquid flow control valve 20 at the bottom of dilution cup 6 and test cup 7 is opened, and the first drain pump 13 works to drain the diluent originally stored in dilution cup 6 and test cup 7. The multi-functional suction needle 2 and diluent 11 work together to add a certain amount of diluent to dilution cup 6 and test cup 7 respectively, and the dilution cup 6 and test cup 7 are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0080] The multi-functional aspiration needle 2, in conjunction with the diluent 11, adds a certain amount of diluent to the release cup 6 and the detection cup 7 respectively, and both cups retain the diluent.

[0081] Step 2: Dilute the sample. The multi-functional aspiration needle 2 is moved into the sample cup. The multi-functional aspiration needle 2 works with the diluter 11 to aspirate a certain amount of blood sample and transfer it to the dilution cup 6. A certain amount of diluent is added to the dilution cup 6, and the blood sample in the dilution cup 6 is mixed by the stirring device.

[0082] Step 3, raw testing: The multi-functional aspiration needle 2 draws a certain amount of diluted blood sample from the dilution cup 6 and transfers it to the test cup 7. A certain amount of diluent is added to the test cup 7. The blood sample in the test cup 7 is mixed by the stirring device. The test cup 7 is tested by the testing device 12 to obtain the information data of platelets in the original state of the blood sample.

[0083] Step 4, waste liquid treatment: the drain channels at the bottom of dilution cup 6 and test cup 7 are opened, and the waste liquid in dilution cup 6 and test cup 7 is discharged through the connecting pipe and the first drain pump 13.

[0084] Step 5: Cleaning device. A certain amount of diluent is added to dilution cup 6 and detection cup 7 respectively through the multi-functional aspiration needle 2 and diluent 11. The dilution cup 6 and detection cup 7 are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0085] Step 6: Using the multi-functional aspiration needle 2 and diluent 11, add a certain amount of diluent to the release cup 6 and the detection cup 7 respectively.

[0086] Step 7, reaction detection: A certain amount of agglutinant is drawn from the reagent bottle using the multi-functional aspiration needle 2 and added to the sample cup after the original blood sample test is completed. The blood sample and reagent in the sample cup are stirred and mixed using a stirring device. After sufficient reaction time, the multi-functional aspiration needle 2 draws the reacted blood sample from the sample cup and performs the test according to steps 1-5 above to obtain information on the aggregation reaction of platelets in the blood sample after treatment with the agglutinant.

[0087] Step 8, data processing: The device compares the changes in platelet count and volume of the blood sample before and after the addition of the agglutinant according to the above process to obtain the platelet function level test result of the blood sample.

[0088] Step 9: Repeat the test. The device repeatedly performs platelet function tests on different blood samples according to steps 1-8 above.

[0089] When a platelet function testing device tests more than one blood sample at the same time, the timing of platelet count testing of different original blood samples and the order of adding agglutinating agents can be optimized to achieve optimized testing of more than one sample, or to complete the testing of different samples one by one according to the above steps to obtain different platelet receptor function test results of different samples, or the same sample with different results.

[0090] Example 2:

[0091] The platelet function testing device calculates platelet function by adding different reagents to the same blood sample and measuring the number of platelets obtained. The workflow includes the following steps:

[0092] Step 1, Pre-processing: Sample cups 101 and 102, each containing the same blood sample, are treated with reagents from different reagent bottles. Sample cup 101 is treated with EDTA, a strong anticoagulant from the first reagent bottle 801, and sample cup 102 is treated with arachidonic acid, a platelet aggregation inducer from the second reagent bottle 802. Each sample is then mixed using a stirring device.

[0093] The third liquid flow control valve 20 at the bottom of dilution cup 6 and test cup 7 is opened, and the first drain pump 13 works to drain the diluent originally stored in dilution cup 6 and test cup 7; the multi-functional suction needle 2 and the diluent 11 work together to add a certain amount of diluent to dilution cup 6 and test cup 7 respectively, and the dilution cup 6 and test cup 7 are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0094] The multi-functional aspiration needle 2, in conjunction with the diluent 11, adds a certain amount of diluent to the release cup 6 and the detection cup 7 respectively, and both cups retain the diluent.

[0095] Step 2: Dilute the sample. The multifunctional aspiration needle 2 is moved into the sample cup 101. The multifunctional aspiration needle 2 and the diluent 11 work together to aspirate a certain amount of blood sample and transfer it to the dilution cup 6. A certain amount of diluent is added to the dilution cup 6, and the blood sample in the dilution cup 6 is mixed by the stirring device.

[0096] Step 3, Sample testing: The multifunctional aspiration needle 2 draws a certain amount of diluted blood sample from the dilution cup 6 and transfers it to the test cup 7. A certain amount of diluent is added to the test cup 7. The blood sample in the test cup 7 is mixed by the stirring device. The test cup 7 is tested by the detection device 12 to obtain information on the platelet status of the blood sample in the blood cup.

[0097] Step 4, waste liquid treatment: the drain channels at the bottom of dilution cup 6 and test cup 7 are opened, and the waste liquid in dilution cup 6 and test cup 7 is discharged through the connecting pipe and the first drain pump 13.

[0098] Step 5: Cleaning device. A certain amount of diluent is added to dilution cup 6 and detection cup 7 respectively through the multi-functional aspiration needle 2 and diluent 11. The dilution cup 6 and detection cup 7 are rinsed by the stirring device. After rinsing, the rinsed diluent is discharged.

[0099] Step 6: Using the multi-functional aspiration needle 2 and diluent 11, add a certain amount of diluent to the release cup 6 and the detection cup 7 respectively.

[0100] Step 7: Repeat steps 2-6 to test the blood sample in sample cup 102 to obtain information on the platelet status of the same blood sample after it has been treated with different reagents.

[0101] Step 8: Based on the differences in test results between the same blood samples with added platelet aggregation inducing agents and strong platelet anticoagulants, compare and obtain the platelet function level test results of the blood sample.

[0102] Step 9: Repeat steps 1-8 to test platelet function in different blood samples.

[0103] In some embodiments, the dilution module includes two diluters, according to... Figure 2 As shown, the dilution module of the device has a diluent 11 and a second diluent 23. The diluent 11 has a larger capacity and is mainly used for aspirating and dispensing the diluent, while the second diluent 23 has a smaller capacity and is mainly used for aspirating and dispensing the blood sample and the diluted blood sample. The two diluents can be driven by a shared motor or by two different motors.

[0104] Furthermore, the multifunctional suction needle tip of the device is also equipped with a needle surface cleaning device 25, the specific structure of which is an annular flow cleaning pool set outside the multifunctional suction needle.

[0105] The blood analyzer designed in this way, when equipped with two diluents, performs the following workflow for sample and internal reagent aspiration and dispensing, as well as diluent aspiration and dispensing: When the multi-functional aspiration needle needs to aspirate a sample or internal reagent, or dilute a well-mixed sample, the first flow control valve 18 is closed and the fifth flow control valve 24 is opened, allowing the piston of the diluent 23 to retract and aspirate the sample, dilute the sample, or the internal reagent; when it is necessary to dispense the sample or reagent aspirated by the multi-functional aspiration needle 2, the piston of the diluent 23 is pushed forward, and the sample aspirated by the multi-functional aspiration needle 2 is ejected.

[0106] When it is necessary to aspirate and dispense the diluent, firstly, both the first flow control valve 18 and the fourth flow control valve 22 are closed, and the second flow control valve 19 is opened. The piston of the diluent 11 is retracted, and the diluent is drawn into the inner cavity of the diluent 11 by the negative pressure generated by the retraction. Then, the second flow control valve 19 is closed, and both the first flow control valve 18 and the fifth flow control valve 24 are opened. The piston of the diluent 11 is pushed forward, and a large positive pressure is generated in the diluent 11. This drives the diluent in the inner cavity of the diluent 11 through the pipeline through the opened first flow control valve 18 and the fifth flow control valve 24, and then through the multi-functional sampling needle 2 to be dispensed into the required target container.

[0107] When the surface of the multifunctional aspiration needle 2 needs cleaning, the first flow control valve 18 and the fourth flow control valve 22 are closed, while the second flow control valve 19 is opened. The piston of the diluent 11 is pulled back to create a negative pressure inside the diluent 11, causing the diluent in the diluent tank 10 to be drawn into the inner cavity of the diluent 11 through the pipeline. Then, the second flow control valve 19 is closed, the fourth flow control valve 22 is opened, and the piston of the diluent 11 is pushed forward, causing the diluent in the inner cavity of the diluent 11 to flow under pressure through the pipeline connected to the fourth flow control valve 22 to the inlet of the multifunctional aspiration needle surface cleaning device 25. At the same time, the third flow control valve 20 is closed, the sixth flow control valve 26 is opened, and the second discharge pump 21 is started, so that the diluent entering the multifunctional aspiration needle surface cleaning device 25 is quickly discharged to achieve the effect of cleaning the surface of the multifunctional aspiration needle.

[0108] This invention provides a concept and method for a lightweight platelet function detection device. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.

Claims

1. A lightweight platelet function testing device, characterized by, The device comprises a sample processing module and a detection module. The sample processing module comprises a multifunctional liquid suction needle (2) for sucking liquid, a driving device and a dilution module for diluting samples; the multifunctional liquid suction needle (2) is connected with the dilution module through a liquid path pipe, and the driving device controls the movement of the multifunctional liquid suction needle (2); the dilution module is connected with a dilution liquid barrel (10) through a pipe. The detection module comprises a sample cup site for placing a sample cup and an internal reagent site for placing a detection reagent, and the sample cup site and the internal reagent site are located on the movement track of the multifunctional liquid suction needle (2).

2. The lightweight platelet function testing device according to claim 1, wherein The detection module comprises a dilution cup (6) and a detection cup (7), the detection cup (7) is provided with a platelet detection device and a stirring device for mixing the diluted sample liquid in the cup, the dilution cup (6) is provided with a stirring device, the lower part of the dilution cup (6) and the detection cup (7) is provided with a liquid discharge port, and the dilution cup (6) and the detection cup (7) are connected with a waste liquid processing module through the liquid discharge port.

3. The lightweight platelet function testing device of claim 2, wherein The waste liquid processing module comprises a third liquid flow control valve (20) and a first liquid discharge pump (13), and the dilution cup (6) and the detection cup (7) are connected with the third liquid flow control valve (20) through a waste liquid discharge pipe (16), and the third liquid flow control valve (20) is connected with the first liquid discharge pump (13) through a waste liquid discharge collection pipe (17).

4. The lightweight platelet function testing device according to claim 3, wherein The stirring device comprises a magnetic stirring device (14) and a magnetic stirring sub (15), the magnetic stirring sub (15) is respectively arranged in the dilution cup and the detection cup, the magnetic stirring device (14) is respectively arranged at the outer bottom of the detection cup and the dilution cup, the magnetic stirring device (14) drives the magnetic stirring sub (15) to move through magnetic force so as to stir the diluted sample in the dilution cup (6) and the detection cup (7); The inner bottom of the dilution cup (6) and the detection cup (7) is respectively provided with a radial shallow groove strip, the radial shallow groove strip is matched with the shape of the magnetic stirring sub (15), the magnetic stirring sub (15) falls into the radial shallow groove strip after the stirring stops, and the magnetic stirring sub (15) is in a stable and static state when not stirring.

5. The lightweight platelet function testing device according to claim 3, wherein The stirring device is a gas mixing needle arranged in the dilution cup (6) and the detection cup (7) and a gas supply pump connected with the gas mixing needle, the gas discharge port of the gas mixing needle is respectively located in the dilution cup (6) and the detection cup (7), and the gas supply pump provides disturbance gas for the gas mixing needle to stir and mix the diluted sample in the dilution cup (6) and the detection cup (7).

6. The light-weight platelet function testing device according to claim 4 or 5, wherein The dilution module comprises a diluter (11) and a dilution liquid barrel (10) arranged outside the device for placing dilution liquid; the multifunctional liquid suction needle (2), the first liquid path pipe (5), the diluter (11), the second liquid path pipe (9) and the dilution liquid barrel (10) are sequentially connected, the first liquid path pipe (5) is provided with a first liquid flow control valve (18), and the second liquid path pipe (9) is provided with a second liquid flow control valve (19).

7. The lightweight platelet function testing device of claim 6, wherein The detection cup (7) and the reagent bottle are respectively provided with an automatic cover opening and closing device, and the automatic cover opening and closing device is matched with the movement of the multifunctional liquid suction needle (2) to open and close.

8. The lightweight platelet function testing device of claim 7, wherein The material of the automatic cover opening and closing device is an external electrical signal interference resistant material.

9. The lightweight platelet function testing device of claim 1, wherein The sample cup site on the platelet function detection device is provided with a magnetic stirring device, and a magnetic stirring rod is arranged in the sample cup.

10. The lightweight platelet function testing device of claim 1, wherein The sample cup of the platelet function detection device is an independent single cup or is formed by at least two same structure cups combined to form a single-row combined sample joint cup structure, and the cups of the combined sample joint cup are independent and not communicated with each other; the height of the sample cup is 12-55 mm, and the inner diameter of the single cup is 5-15 mm.

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