144 results about "PLT - Platelets" patented technology

Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation, and to detect back-scattered (reflected) radiation using a plurality of optical fibers to transmit the back-scattered radiation to a high-gain photodetector, e.g. a photomultiplier tube. Preferably, the back-scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter within a prescribed, relatively narrow angular range, or the level of side-scattered radiation, or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent/stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

A microfluidics device to provide real time monitoring of platelet aggregation of a biological sample obtained from a subject. The device comprises a channel configured for passage of the biological sample, the channel comprising a protrusion configured to induce an upstream region of shear acceleration coupled to a downstream region of shear deceleration and defining there-between a region of peak rate of shear, the downstream region of shear deceleration defining a zone of platelet aggregation. The device further comprises a platelet detection means for detecting aggregation of platelets in the zone of aggregation as a result of passage of the biological sample through the channel. Methods to assess real time platelet aggregation of a biological sample obtained from a subject are further described.

The invention discloses a platelet oscillating storage box, which includes a box body. The box body is provided with six circular array and connected rotating cavities. There are five storage blocks in the rotating cavity, and the storage blocks are provided with storage. A cavity, a storage block is fixed on the lower end wall of the storage cavity, and an end of the storage cavity close to the center of the array is provided with a fixed block that is symmetrical up and down, and a movable clamping block is provided between the fixed blocks. The clamping device can be used to clamp the test tube containing the platelets in the storage cavity, and the storage cavity is independent and communicates with the thermostat independently. When taking or storing platelets, the thermostatic conditions in the storage cavity to be placed are blocked. Cut off, reducing energy loss, without destroying the constant temperature environment in other storage chambers, and prolonging the storage time of platelets. Secondly, the sealing device can keep the storage chamber sealed, and the sealing cover and the storage chamber remain locked during the oscillation process. Ensure constant temperature in the storage chamber.

The invention discloses a sepsis early prediction method based on machine learning. The method comprises the following steps: firstly, extracting clinical data, including demographic statistics (suchas age and gender), vital sign variables (such as heart rate and systolic pressure) and laboratory measurement indexes (such as creatinine and platelet count), of a patient within 24 hours after a patient enters an ICU by utilizing an electronic medical record, preprocessing the data, inputting the preprocessed data into an improved deep forest algorithm model for training, and outputting the disease probability of the patient after training optimization. And meanwhile, the algorithm model can also sort characteristic variables and output an early warning factor which has great influence on early prediction of sepsis. Finally, corresponding variables of the patient needing to be predicted are input into the trained model, so that early prediction of sepsis can be carried out on the patient. According to the invention, early prediction is carried out on sepsis based on a machine learning method, doctors can be assisted in making clinical decisions, and the prediction accuracy is improved.

The present invention refers to a novel device for measuring coagulation time and platelet activity wherein the patient can measure his or her coagulation time and platelet activity without the aid of medical professionals due to the fact that this device is fully autonomous. A blood sample (14) is deposited in the dish (3) and reacted with a reactant (16). The display (9) then shows the coagulation time and platelet activity of the patient.

The invention belongs to the technical field of preparation of platelet rich plasma, and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a manual two-step centrifugation method. The method comprises the following steps: centrifuging 25 ml of whole blood mixed with an anticoagulant for 6 minutes at centrifugal force of 800 g for the first time by adopting the two-step centrifugation method, absorbing plasma on the upper layer and on boundary layer in the layered whole blood, and arranging the plasma in an another centrifugal tube; centrifuging for the second time, namely centrifuging for 5 minutes at centrifugal force of 1400 g, and absorbing about 4 ml of the layered plasma on the middle layer to obtain the platelet rich plasma. According to the PRP prepared by the technology, the platelet concentration is 886.33*10<9>/L, the mean corpuscular hemoglobin in the PRP is 27.72 g/L, and the clinical treatment requirements are met.

The invention provides an optimized automation-adaptable platelet aggregation function inspection and analysis method, and belongs to the field of clinical laboratory medicine. The method is an improvement and optimization of a platelet aggregation function inspection and analysis method using an automation counting method. The method is suitable for platelet aggregation function automatic analysis instruments using the counting method. According to the method, the number of platelets is inspected and recorded and an aggregation rate is calculated. A laboratory test result shows that the method is better in repeatability of the obtained result of platelet aggregation rate compared with a previous largest aggregation rate method.

A method, system and storage medium for providing an alarm for indicating that an abnormality is present in a sample analyzer are provided. The method includes: mixing a first aliquot of a blood sample with a diluent agent to prepare a first test sample; mixing a second aliquot of the blood sample with a lytic reagent to prepare a second test sample; detecting electrical impedance signals of the first test sample; detecting at least two types of optical signals of the second test sample; acquiring first platelet detection data based on the electrical impedance signals; acquiring second platelet detection data based on the at least two types of optical signals; acquiring an evaluation result based on a difference between the first platelet detection data and the second platelet detection data; determining whether the evaluation result meets a preset condition to provide an alarm.

The invention relates to a method for detecting biological activity of resina draconis, which realizes control on whole efficacy of complex components of the resina draconis. The method comprises the following steps of: crushing the resina draconis into ethanol, carrying out ultrasonic extraction, drying the extracts, using dimethyl sulfoxide for dissolving to obtain solution, continuously diluting, and obtaining solution with geometric proportion concentration; respectively measuring blood plasma recalcification time contraction rate and platelet aggregation inhibition ratio of the resina draconis; and carrying out titer calculation according to a parallel line assay method based on quantitative responses (2.2) of appendix X IV of Chinese Pharmacopoeia II. The method is simple and easy to operate, is accurate to measure, and can be used for controlling the whole efficacy of the resina draconis with undefined active constituents.

An optical detection system, a blood cell analyzer and a platelet detection method are provided. The optical detection system includes: an optical subsystem, a flow chamber and a first detector; the optical subsystem includes a laser, a front optical assembly including an optical isolator, and a rear optical assembly including a blocking diaphragm. The laser is configured to emit a laser beam; the front optical assembly is configured to perform front optical treatment; the rear optical assembly is disposed downstream of the flow chamber in the propagation direction of the laser beam, and is configured to perform rear optical treatment on the scattered light and the laser beam converged at the blocking diaphragm; and the optical isolator is configured to isolate reflected light that is generated when the laser beam passes through the flow chamber.

The invention relates to the technical field of medical instruments, in particular to a platelet-rich plasma collecting and separating device adopting physical (freezing) anticoagulation, which comprises a plugging screw cap and an outer injector, an inner injector is inserted in the outer injector, a piston assembly is arranged at the bottom of the inner injector and is positioned in the outer injector. A fixed anti-rotation frame is integrally formed on the top of the piston assembly. The plasma prepared by the device and the method disclosed by the invention is pure (without exogenous anticoagulant and other additives) and has different convertible use modes: the separated plasma is in a liquid state within 30 minutes at a low temperature (above a freezing temperature), the liquid plasma can be converted into jelly-shaped gel along with temperature and time changes, different clinical requirements can be met, and the application space is expanded. By utilizing the device and the method disclosed by the invention, platelet plasma or gel with different concentrations can be prepared only by strictly mastering three elements of temperature, time and centrifugal force.

The invention discloses a micro-platelet RNA sequencing detection kit based on BGISEQ-500. The invention also provides a method for constructing a platelet RNA sequencing library based on BGISEQ-500.The method comprises the following steps: 1) extracting platelet RNA from a sample to be detected, and performing reverse transcription to obtain dscDNA; 2) fragmenting the dscDNA by a linker-embeddedTn5 transposase to obtain a fragmented product; 3) carrying out PCR amplification of the fragmented product to obtain an amplification product; and 4) cyclizing the amplification product to obtain the platelet RNA sequencing library. Experiments prove that below 1 ml of whole blood is adopted to construct the micro-platelet mRNA library, and an autonomously established platelet analysis procedureis used to carry out highly-sensitive and highly-specific early screening of tumors.

Disclosed are a blood analysis system, a blood analyzer, a blood analysis method and a storage medium. The blood analysis method includes: acquiring at least two types of optical signals of a test sample derived from a blood sample, wherein red blood cells in the test sample are lysed, blood cells in the test sample are stained by a fluorescence dye, and the at least two types of optical signals include scattered light signals and/or fluorescent signals; generating a scattergram based on the at least two types of optical signals; identifying a preset region in the scattergram based on the at least two types of optical signals; and acquiring detection data of a platelet subpopulation based on the preset region.

The invention discloses a detection device based on a blood coagulation and platelet function analyzer. The device comprises a machine body; to-be-tested agent cup slots are uniformly formed in the middle position of the upper surface of the machine body; a detection piece and a printer are respectively arranged on the upper surfaces of the two sides of the machine body; interfaces are uniformly formed in the rear surface of the machine body; and one side surface of the machine body is fixedly connected with a paper storage mechanism. The relates to the technical field of blood coagulation and platelet detection. According to the detection device based on the blood coagulation and platelet function analyzer, the elasticity of a first spring and arc-shaped sheets is utilized, when blood coagulation and platelets in a reagent bottle need to be detected, the two arc-shaped sheets are pinched so that clamping blocks on two sliding rods are opened, the reagent bottle is placed in an annular cylinder, then the arc-shaped sheets are loosened, and the clamping blocks on the two sliding rods can firmly clamp the two sides of the reagent bottle, so that a worker can better detect blood coagulation and blood platelets in the reagent bottle.

The invention relates to a medical separation and extraction device, which comprises a first container, a second container and a third container, wherein the first container comprises a first outer wall, a first cavity and at least a communication opening penetrating through the first outer wall, the second container comprises a second outer wall, a second adjusting member and a second cavity, thethird container comprises a third outer wall, a third adjusting member and a third cavity, the volumes of the second cavity and the third cavity are correspondingly changed along with the sliding ofthe second adjusting member and the third adjusting member respectively, the second cavity and the third cavity can be communicated with the first cavity respectively through the communication opening, the third container is detachably connected to the first container, and the second container is provided with a port capable of directly extracting blood through an injection needle. According to the medical separation and extraction device of the invention, the whole sucking, separation and extraction process can be realized by only adopting the device under the conditions of liquid collectionwith no use of additional injection needle and sucking storage of the extract, so that the operation is simple, and the pollution risk is reduced.

Methods to significantly reduce clot formation in cold-stored platelet samples are described. The methods include collecting platelet samples at defined yields and/or concentrations and allowing collected platelet samples to rest at room temperature without agitation for a period of time before being moved into cold storage.