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Improved self platelet-rich plasma preparation method

A platelet-rich plasma and blood bag technology, applied in the biological field, can solve the problems of high cost and high pollution risk, and achieve the effects of low pollution risk, simple preparation method and cost reduction.

Inactive Publication Date: 2018-11-06
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing cost is high and the risk of pollution is high

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0013] An improved autologous platelet-rich plasma preparation method, including peripheral venous blood collection and autologous PRP preparation:

[0014] (1) The details of peripheral venous blood collection are as follows:

[0015] 1) The patient puts on shoe covers and enters the blood collection room (it can also be collected at the bedside of the clinical department). Blood collectors should dress neatly, wash hands, and wear masks;

[0016] 2) Assess the blood vessel condition;

[0017] 3) Confirm that the ID card is the patient himself, and check the relevant information;

[0018] 4) Check the expiry date of the blood collection device, whether it is damaged, whether the preservation solution is clear, and confirm that it is available; if the planned collection volume is less than 200ml, part of the maintenance solution can be properly removed in proportion (the ratio of maintenance solution to whole blood is 1:4);

[0019] 5) Expose the arm on the collection side,...

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Abstract

The invention relates to an improved self platelet-rich plasma preparation method. A centrifugation method is adopted two times, centrifugation is performed under the condition of 850-1875 g for 11-15minutes for the first time, and centrifugation is performed under the condition of 100 g for 12 minutes for the second time. The method is simple, the platelet recovery rate is high, the cost is low,the preparation process is closed, the pollution risk is minimized, the product quantity and platelet number can be flexibly adjusted according to clinic demands, and a new method is provided for clinic preparation of self platelet-rich plasma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an improved preparation method of self-platelet-rich plasma. Background technique [0002] Platelet-rich plasma (Platelet-rich plasma, PRP), centrifuged autologous whole blood to obtain plasma components containing high concentrations of platelets. Platelets contain a large number of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), insulin-like growth factor (IGF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) Wait. It has the effect of promoting cell growth and repairing bone defects. Therefore, platelet-rich plasma has been widely used in the research and experimental treatment of bone defects, soft tissue injury repair, wound healing, infection treatment, functional reconstruction of skin chronic ulcers and other fields. However, the existing cost is high and the risk of pollution is high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D17/038
CPCB01D17/0217
Inventor 陆华易中梅徐婷李晨晨
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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