A reagent for stabilizing blood sample cells

A blood sample and cell technology, which is applied in the preparation of test samples, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of no anticoagulant preservation reagents, etc., and achieve the effect of preventing platelet aggregation and stabilizing blood cells

Active Publication Date: 2016-05-11
广东国盛医学科技有限公司 +1
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Problems solved by technology

[0004] It should be further pointed out that for fine systems for detecting blood cells, such as microfluidic platforms and flow cell separation instruments, it is necessary to be able to prevent blood cell aggregation and blood coagulation, that is, to avoid blood sample clogging; however, there is no effective Anticoagulant Preservation Reagent

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Embodiment Construction

[0021] The present invention will be described below in conjunction with specific embodiments.

[0022] A reagent for stabilizing cells of a blood sample, comprising materials having the following concentrations, specifically:

[0023] β-cyclodextrin 100μmol / L

[0024] Disodium Cromoglycate 100μmol / L

[0025] Potassium dichromate 0.05%

[0026] Triflusal 50μmol / L

[0027] Trutroban 100μmol / L

[0028] Sodium citrate 11mmol / L

[0029] Theophylline 1.5mmol / L

[0030] Dipyridamole 20μmol / L

[0031] Adenosine 0.37mmol / L

[0032] Glucose 20mmol / L

[0033] PEG1.25mmol / L.

[0034] For further explanation, the PEG of the present invention is PEG-1000.

[0035] Among them, for the reagents for stabilizing blood sample cells of the present invention, β-cyclodextrin, disodium cromoglycate, and potassium dichromate can directly stabilize blood cell membranes, and triflusal, trutroban, theophylline, dipyrim Damo can effectively protect platelets and inhibit platelet aggregation, ...

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Abstract

The present invention disclosed a reagent that stabilizes blood sample cells, including the following concentration materials: 100 μmol / lβ-cycloped, 100 μmol / L di-sodium glycolic acid, 0.05%potassium chromate, 50 μmol / L triterfluilow,100 μmol / L Truquan, 11mmol / L citric acid, 1.5mmol / L catechine, 20 μmol / L ductoid Damo, 0.37mmol / L adenosine, 20mmol / L glucose, 1.25mmol / L?PEG.The present invention can stabilize blood cells and prevent blood plates from gathering and stabilize blood cells for at least 36 hours. The treated blood samples will not affect subsequent sample molecular analysis. In addition to being widely used in blood sample molecular analysis and testing, it is also suitable for sample cells to be used for sample cellsCells or molecular detection and analytical technology platforms with strict requirements, such as micro -current control technology, streaming cytometer, etc.

Description

technical field [0001] The invention relates to the technical field of blood preservation reagents, in particular to a reagent for stabilizing blood sample cells. Background technique [0002] Stable blood samples are extremely important for the transportation of blood samples and the operation of the clinical trial process, there are some detection techniques and methods, such as enrichment and analysis of rare circulating cells (circulating tumor cells, circulating fetal cells, circulating stem cells and circulating vascular endothelial cells) or The detection of pathogenic bacteria in blood usually requires multi-step and complex enrichment processes. Stabilizing blood cells and enriching target cells that can withstand the enrichment process without cell dissolution are the key to the successful development of such products. [0003] Formalin has been used as a fresh-keeping agent for tissues and cells for nearly a hundred years. Among them, formalin is a preservative th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02G01N1/28
Inventor 邓杏飞
Owner 广东国盛医学科技有限公司
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