PLT particle detection method and device of blood cell analyzer

Abstract

The invention discloses a PLT particle detection method and device for a blood cell analyzer, and the method comprises the steps: carrying out the classification of a pulse signal according to a plurality of classification precisions, obtaining a plurality of classification histograms with the classification precisions, segmenting the classification histogram with each classification precision into a preset number of reference sub-classification histograms according to a reference boundary, correcting the reference boundary in each reference sub-classification histogram to obtain an accurate sub-classification histogram, determining the number of platelet aggregation particles based on the corresponding sub-classification histograms in all the classification histograms, determining an accurate first boundary according to a preset display histogram function and the number of platelet aggregation particles, so that the platelet aggregation particles and the red blood cell particles can be distinguished more accurately in the classification histogram.

Description

technical field [0001] The invention relates to the technical field of blood cell analyzers, in particular to a method and device for detecting PLT particles of a blood cell analyzer. Background technique [0002] Blood cell analyzer is an instrument that can detect cells in blood, and can count and classify white blood cells, red blood cells, nucleated red blood cells, reticulocytes and other cells. [0003] The most common method for blood cell analyzers to detect platelets is the impedance detection method. The blood sample is diluted and mixed in a conductive diluent, and then the diluent flows through the small holes with electrodes at both ends. When the cell particles pass through the small hole, the voltage at both ends of the small hole will generate a voltage pulse due to the change. The larger the volume of the cell particle, the larger the voltage pulse will be. By recording the voltage pulse value of each cell particle, it will have the same voltage pulse The v...

AI Technical Summary

Problems solved by technology

[0004] However, some blood samples are samples containing small red blood cells or samples with red blood cell fragments. Small red blood cells and red blood cell fragments are easily detected as platelets due to their similar volume to platelets, and huge platelets are also easily mistaken for red blood cells. The histogram of the voltage pulse value-pulse number of the blood sample has no obvious features, please refer to figure 2 , figure 2 It is the histogram of the voltage pulse value-pulse number of abnormal blood samples, and the PLT-RBC boundary line is difficult to accurately distinguish platelet aggregation particles (PLT particles) from red blood cell particles (RBC particles), so that the detection accuracy of platelets is limited. great influence

Images