Method for detecting biological activity of resina draconis

A technology of biological activity and dragon's blood, applied in the field of medicine, can solve the problems of not reflecting biological activity and curative effect, complex structure, etc.

Inactive Publication Date: 2012-09-12
中国医学科学院药用植物研究所云南分所
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Problems solved by technology

[0004] The biological activity assay is suitable for traditional Chinese medicines with diverse components and complex structures, and physical and chemical assays cannot reflect biological activity and curative effect

Method used

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  • Method for detecting biological activity of resina draconis
  • Method for detecting biological activity of resina draconis
  • Method for detecting biological activity of resina draconis

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Embodiment Construction

[0017] The present invention will be described in detail below through examples.

[0018] Modern pharmacological studies have shown that dragon's blood has a two-way regulating effect in the treatment of TCM anemia: it can not only improve the body's microcirculation, activate blood circulation and remove blood stasis, but also increase blood coagulation factors in the body, and has a significant hemostatic effect on bleeding patients.

[0019] The present invention uses the following instruments and reagents: Centrifuge, Xiangzhi Centrifuge Factory, TGL16M type; Analytical Balance, Mettler Toledo Shanghai Co., Ltd., PL203 type; Ultrasonic Extractor, Ningbo Haishu Wufang Ultrasonic Equipment Co., Ltd., WF- 500E type; platelet aggregation analyzer, the United States, AC9000 type.

[0020] Adenosine diphosphate sodium salt, calcium chloride, ethanol, and dimethyl sulfoxide are commercially available and of analytical grade.

[0021]

[0022] The present invention implements co...

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Abstract

The invention relates to a method for detecting biological activity of resina draconis, which realizes control on whole efficacy of complex components of the resina draconis. The method comprises the following steps of: crushing the resina draconis into ethanol, carrying out ultrasonic extraction, drying the extracts, using dimethyl sulfoxide for dissolving to obtain solution, continuously diluting, and obtaining solution with geometric proportion concentration; respectively measuring blood plasma recalcification time contraction rate and platelet aggregation inhibition ratio of the resina draconis; and carrying out titer calculation according to a parallel line assay method based on quantitative responses (2.2) of appendix X IV of Chinese Pharmacopoeia II. The method is simple and easy to operate, is accurate to measure, and can be used for controlling the whole efficacy of the resina draconis with undefined active constituents.

Description

technical field [0001] The invention relates to medicine, in particular to a method for detecting biological activity of dragon's blood. Background technique [0002] The current method of quality control of traditional Chinese medicine is qualitative or quantitative detection of some index components. However, due to the complexity of the ingredients in traditional Chinese medicine and the unclear effective substances, it is difficult for this detection method to ensure the stability of the quality of traditional Chinese medicine. associated with safety and efficacy. The outline of the 2010 edition of the "Chinese Pharmacopoeia" pointed out that the quality standard system of traditional Chinese medicine should transition from qualitative and quantitative single index components to comprehensive detection of activity and biological assays. [0003] Dracaena is a resin extracted from the fat-containing wood of Dranaena cochinchinensis (Lour.) S.C.Chen, a plant of the Liliac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
Inventor 李宜航陈曦李光李学兰马小军宋美芳赵俊凌
Owner 中国医学科学院药用植物研究所云南分所
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