Method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation

A technology related to function and function detection, applied in measurement devices, biological tests, material inspection products, etc., can solve the problem of inability to detect blood coagulation function detection by platelet aggregation function

Inactive Publication Date: 2014-01-15
SINNOWA MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the strong chelating effect of EDTA on calcium ions, EDTA anticoagulant blood cannot be used for platelet aggregation function detection and other coagulation function detection according to routine operations.

Method used

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  • Method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation

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Experimental program
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Effect test

specific Embodiment 1

[0004] Taking platelet counting method to detect platelet aggregation function as an example, the specific operation process of this method is: when collecting fresh blood samples, prepare anticoagulant blood according to the conventional ratio (add 0.8mgEDTA per 1ml whole blood), and then use this EDTA anticoagulant blood Perform platelet count to obtain the original number of platelets in the blood sample, then add calcium ion-containing aggregation inducer to the blood sample, and the final content of added calcium chloride is >2.0mmol / L. In normal blood samples, platelets begin to aggregate after adding calcium-containing aggregation inducers, and the platelet aggregation rate can be judged by detecting the number of aggregated platelets, and platelet aggregation does not occur in normal blood samples without adding calcium ions and simply adding aggregation inducers. It shows that conventional EDTA anticoagulant blood has no aggregation function under the condition of not ...

specific Embodiment 2

[0011] Five parts of EDTA anticoagulant blood collected were prepared into plasma, and each part was divided into two parts at the same time to form two groups. One group was added with calcium chloride-containing PT (prothrombin time test) reagent, and the other group was added with the same amount Does not contain calcium PT (prothrombin time test) reagents, etc. Two groups of samples were added with PT (prothrombin time test) reagent for 5 minutes to observe the plasma coagulation status of the two groups. The result statistics are as follows:

[0012] Table 2 Observation table for the number of platelets obtained by using EDTA anticoagulated blood samples plus PT (prothrombin time test) reagent

[0013] Numbering

[0014] *Indicating that EDTA anticoagulant plasma can restore blood coagulation function after adding calcium. That is, EDTA anticoagulant blood can be tested for blood coagulation function after adding calcium ions.

specific Embodiment 3

[0015] Calcium ions were added to EDTA anticoagulated whole blood, and the blood samples were all coagulated to varying degrees after standing still for 10 minutes.

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Abstract

The invention is a method for coagulation related function detection by EDTA (ethylene diamine tetraacetic acid) anticoagulation, and belongs to the field of medical in vitro diagnosis industry. According to the method, the EDTA anticoagulation can be used for blood cell count by a blood analyser, and can be used for detection of platelet aggregation and other blood coagulation functions.

Description

1. Technical field [0001] The invention relates to a method for detecting blood coagulation-related functions with EDTA anticoagulant blood. The inventive method enables the EDTA anticoagulant blood not only to be used for counting and analyzing blood cells by a blood analyzer, but also for detection of platelet aggregation and the like. The invention belongs to the field of medical in vitro diagnosis industry. 2. Background technology [0002] EDTA (dipotassium ethylenediaminetetraacetic acid) anticoagulant blood is an anticoagulant blood preparation method commonly used in clinical blood sample routine examination, especially suitable for blood cell analysis and blood sample analysis, and can obtain satisfactory cell counting results. The International Committee for Standardization of Hematology (ICSH) recommended EDTA as an anticoagulant for blood cell counts in 1993. However, due to the strong chelating effect of EDTA on calcium ions, EDTA anticoagulant blood cannot be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N15/10
CPCG01N33/5005G01N33/86
Inventor 徐新
Owner SINNOWA MEDICAL SCI & TECH
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