Cell induction separation device

Abstract

The utility model discloses a cell induced separation device, which belongs to the technical field of cell separation, and adopts the technical scheme that the cell induced separation device comprises a base, the top of the base is fixedly connected with a frame, and a first cleaning pool, a second cleaning pool and an operation table are sequentially arranged in the frame from left to right, and an ultrasonic generating device is arranged in the first cleaning pool; by arranging the flushing assembly, the flushing assembly can spray flushing fluid to the culture chamber from multiple angles, the surface of the culture chamber can be comprehensively and efficiently flushed, so that pollutants such as residual cell debris and an inducer are rapidly removed, the cleaning efficiency is greatly improved, and the cleanliness of the culture chamber is ensured; according to the cell culture device, the culture chamber can be accurately moved into the flushing assembly to be flushed, then moved into the first cleaning pool to be deeply cleaned and then moved to the operation table to be subjected to subsequent cell induction or separation operation, and the use convenience is greatly improved through the flexible position adjusting function.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell separation technical field, especially in a kind of cell induction separation device. BACKGROUND

[0002] In modern cell biology research and biological pharmacy and other related fields, cell induction separation is a key operation process, and the existing cell induction separation mode is usually as follows: in the cell culture stage, cells are inoculated in culture chamber, to make cell growth and reproduction enter induction link, then specific inducer is added to culture chamber, when cell induction is completed, it enters separation stage, according to cell characteristics and experimental purpose, optical tweezers, microfluidic chip and other technical means are selected to separate target cells, such as optical tweezers technology, the light trap force generated by highly focused laser beam is used to accurately control cells, and microfluidic chip realizes separation based on the size, charge and other differences of cells through micro-pipe and microstructure in chip.

[0003] In cell induction separation experiment, different batches of cells can have different characteristics and processing requirements, if culture chamber is not cleaned, the cell debris, inducer and other pollutants of previous batch are mixed into next batch, and currently culture chamber often uses single cleaning mode, it is difficult to completely remove complex and diverse pollutants in culture chamber, so that dry inducer, cell debris and other pollutants can be attached to micro-nano structure or inner wall of culture chamber for a long time, which can cause physical damage to culture chamber, and then affect separation effect.

[0004] Therefore, a cell induction separation device is provided. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a cell induction separation device, which can solve the problem that the existing culture chamber often uses single cleaning mode, it is difficult to completely remove complex and diverse pollutants in culture chamber, so that dry inducer, cell debris and other pollutants can be attached to micro-nano structure or inner wall of culture chamber for a long time, which can cause physical damage to culture chamber, and then affect separation effect.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a cell induction separation device, comprising a base, the top of the base is fixedly connected with a frame, and the inside of the frame is sequentially provided with a first cleaning pool, a second cleaning pool and an operation table from left to right, the inside of the first cleaning pool is provided with an ultrasonic wave generating device, the inside of the second cleaning pool is provided with a flushing assembly, the inside of the frame is provided with an auxiliary cleaning assembly, and the auxiliary cleaning assembly comprises a supporting plate and a connecting shaft.

[0007] The flushing assembly component comprises a water pump, the water pump is fixedly connected at the rear side of the frame, and the water suction end of the water pump extends to the inside of the second cleaning pool; both sides of the inside of the second cleaning pool are fixedly connected with pipes, and both sides close to each other of the two pipes are fixedly connected with high-pressure flushing nozzles; the number of the high-pressure flushing nozzles is multiple and is arranged in an inclined manner; the water outlet end of the water pump is fixedly connected with a metal shunt pipe, the metal shunt pipe penetrates through the frame and is fixedly connected with the pipes.

[0008] Preferably, the top end of the connecting shaft is rotatably connected in the inside of the supporting plate, the top of the supporting plate is fixedly connected with a U-shaped frame, the inside of the U-shaped frame is fixedly connected with a driving motor, and the output end of the driving motor penetrates through the supporting plate and is fixedly connected with the top end of the connecting shaft.

[0009] Preferably, the surface of the bottom end of the connecting shaft is fixedly connected with connecting frames, the number of the connecting frames is several and is arranged in a ring shape, and the top of the connecting frame is fixedly connected with a culture cavity.

[0010] Preferably, the rear side of the inner wall of the frame is provided with a groove, the inside of the groove is rotatably connected with an electric screw rod, the surface of the electric screw rod is threadedly connected with a moving block, the front side of the moving block is fixedly connected with a mounting plate, the top of the mounting plate is fixedly connected with an air cylinder, and the telescopic end of the air cylinder penetrates through the mounting plate and is fixedly connected with the top of the U-shaped frame.

[0011] Preferably, the left side of the first cleaning pool is fixedly connected with a drain pipe, the drain pipe penetrates through the frame and extends to the outside of the frame.

[0012] Preferably, the front side of the operation table is provided with an integrated controller, and the ultrasonic wave generating device, the electric screw rod and the water pump are electrically connected with the integrated controller.

[0013] Preferably, the inside of the second cleaning pool is slidably connected with a movable filter screen frame, and the water suction end of the water pump is located below the movable filter screen frame.

[0014] Preferably, the inside of the frame is fixedly connected with a guide sliding rod, and the mounting plate is slidably connected on the surface of the guide sliding rod.

[0015] Compared with the prior art, the utility model has the advantages that:

[0016] 1、The flushing assembly is arranged, the flushing assembly can spray flushing liquid to the culture cavity from multiple angles, the surface of the culture cavity can be comprehensively and efficiently flushed, residual cell fragments, inducers and other pollutants can be quickly removed, the cleaning efficiency is greatly improved, the cleanliness of the culture cavity is ensured, and a good environment is provided for cell culture and induction;

[0017] 2, The application can realize accurate movement of the culture chamber to the inside of the flushing assembly to receive flushing, then move to the first cleaning pool for deep cleaning, and then move to the operation table for subsequent cell induction or separation operation. This flexible position adjustment function greatly improves the convenience of device use, optimizes the overall cleaning and experimental process, and reduces the error and pollution risk caused by manual operation. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a whole structure diagram of the cell induction and separation device of the utility model.

[0019] Figure 2 It is a top view of the utility model. Figure 1

[0020] Figure 3 It is a structure schematic view of the flushing assembly of the utility model.

[0021] Figure 4 It is a structure schematic view of the auxiliary cleaning assembly of the utility model.

[0022] Figure 5 It is an enlarged schematic view of A in the utility model. Figure 1

[0023] In the drawing, 1, base; 2, frame; 3, first cleaning pool; 4, second cleaning pool; 5, operation table; 6, ultrasonic wave generating device; 7, flushing assembly; 701, water pump; 702, conduit; 703, high-pressure flushing nozzle; 704, metal shunt pipe; 8, auxiliary cleaning assembly; 801, support plate; 802, connecting shaft; 803, U-shaped frame; 804, driving motor; 805, connecting frame; 806, culture chamber; 9, groove; 10, electric screw; 11, moving block; 12, mounting plate; 13, air cylinder; 14, drain pipe; 15, integrated controller; 16, moving filter screen frame; 17, guide slide bar. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0025] Please refer to Figures 1-5 The utility model provides technical scheme:

[0026] ​​The utility model provides a cell induction separation device, including base 1, the top of base 1 is fixedly connected with frame 2 and the inside of frame 2 is sequentially provided with first washing pool 3, second washing pool 4 and operation table 5 from left to right, the inside of first washing pool 3 is provided with ultrasonic wave generating device 6, the inside of second washing pool 4 is provided with flushing subassembly 7, the inside of frame 2 is provided with auxiliary cleaning subassembly 8, and auxiliary cleaning subassembly 8 includes support plate 801 and connecting shaft 802;

[0027] Flushing subassembly 7 component includes water pump 701, water pump 701 is fixedly connected at the rear side of frame 2 and the water suction end of water pump 701 extends to the inside of second washing pool 4, both sides in the inside of second washing pool 4 are fixedly connected with pipe 702 and both pipe 702 are fixedly connected with high pressure flushing nozzle 703 on the side close to each other, the number of high pressure flushing nozzle 703 is set to multiple and is inclined to set, and the water outlet end of water pump 701 is fixedly connected with metal shunt pipe 704, metal shunt pipe 704 penetrates frame 2 and is fixedly communicated with pipe 702.

[0028] In the embodiment: by setting into flushing subassembly 7, the water suction end of water pump 701 can extract the flushing liquid in second washing pool 4, and the flushing liquid is output at a certain pressure, to ensure that the flushing liquid can be smoothly delivered to high pressure flushing nozzle 703 for jet cleaning, one end of metal shunt pipe 704 is connected with the water outlet end of water pump 701, the other end penetrates frame 2 and is connected with pipe 702, it can evenly shunt the flushing liquid output by water pump 701 to both sides pipe 702, to ensure that each part of high pressure flushing nozzle 703 can obtain equal and stable flushing liquid supply, to make the flushing effect more balanced, pipe 702 is installed in both sides in the inside of second washing pool 4, is fixedly communicated with metal shunt pipe 704 and high pressure flushing nozzle 703, bears the task of guiding flushing liquid from metal shunt pipe 704 to nozzle, to ensure that the flushing liquid is accurately delivered to high pressure flushing nozzle 703, to realize the overall flushing of culture chamber 806, and the high pressure flushing nozzle 703 arranged obliquely can jet flushing liquid to culture chamber 806 from multiple angles, to cover the surface of culture chamber 806 in all directions, and the strong flushing force can quickly flush away cell fragments, loose inducer and other pollutants, to greatly improve the flushing efficiency and cleaning effect.

[0029] Specifically, as shown in Figure 4 The top end of connecting shaft 802 is rotatably connected in the inside of support plate 801, the top of support plate 801 is fixedly connected with U-shaped frame 803, U-shaped frame 803 is fixedly connected with drive motor 804 in the inside, and the output end of drive motor 804 penetrates support plate 801 and is fixedly connected with the top end of connecting shaft 802.

[0030] Specifically, as shown in Figure 4As shown, a connecting frame 805 is fixedly connected to the bottom surface of the connecting shaft 802. The number of connecting frames 805 is set to several and they are arranged in a ring. A culture chamber 806 is fixedly connected to the top of the connecting frame 805.

[0031] Specifically, such as Figure 1 , Figure 4 , Figure 5 As shown, a groove 9 is provided on the rear side of the inner wall of the frame 2. An electric lead screw 10 is rotatably connected inside the groove 9. A moving block 11 is threadedly connected to the surface of the electric lead screw 10. An installation plate 12 is fixedly connected to the front side of the moving block 11. A cylinder 13 is fixedly connected to the top of the installation plate 12. The telescopic end of the cylinder 13 passes through the installation plate 12 and is fixedly connected to the top of the U-shaped frame 803.

[0032] In this embodiment: by setting up the auxiliary rinsing component 7, the electric lead screw 10 can drive the moving block 11 to move flexibly on its surface by rotation, so that the auxiliary cleaning component 8 can move horizontally as a whole. This allows the culture chamber 806 to accurately switch positions between different areas such as the rinsing component 7, the ultrasonic cleaning device, and the operating table 5, greatly improving the accuracy and convenience of operation. The mounting plate 12 can provide a mounting base for the cylinder 13, which can raise and lower the auxiliary cleaning component 8. When it is necessary to place the culture chamber 806 into the first cleaning pool 3 and the second cleaning pool 4 for cleaning, or from the first... When the contents are removed from the cleaning tank 3 and the second cleaning tank 4 and transferred to the operating table 5, the cylinder 13 adjusts the height of components such as the U-shaped frame 803 and the connecting shaft 802 by telescopic adjustment, which can ensure that the culture chamber 806 can accurately enter and exit different areas. The support plate 801 can provide rotational support for the connecting shaft 802. When the drive motor 804 starts, it will drive the connecting shaft 802, the connecting frame 805 and the culture chamber 806 to rotate, so that all surfaces of the culture chamber 806 can fully contact the cleaning liquid during the cleaning process, effectively solving the problem of cleaning dead corners, improving the cleaning effect, and meeting the needs of different cleaning methods for all-round cleaning of the culture chamber 806.

[0033] Specifically, such as Figure 1 As shown, a drain pipe 14 is fixedly connected to the left side of the first cleaning pool 3. The drain pipe 14 passes through the frame 2 and extends to the outside of the frame 2.

[0034] Specifically, such as Figure 1 , Figure 3 , Figure 5 As shown, an integrated controller 15 is installed on the front side of the control panel 5. The ultrasonic generator 6, the electric lead screw 10, and the water pump 701 are all electrically connected to the integrated controller 15.

[0035] In the embodiment, through the above setting, the drain pipe 14 can discharge the contaminated cleaning liquid in the first cleaning pool 3, prevent the accumulation of the contaminated cleaning liquid in the first cleaning pool 3, and affect the subsequent cleaning effect. The integrated controller 15 can adjust the frequency, power, and cleaning time of the ultrasonic wave generating device 6, and according to the material, pollution degree, and cell type of the culture chamber 806, accurately control the intensity and duration of ultrasonic cleaning to achieve the best cleaning effect. The electric screw rod 10 can be flexibly controlled to ensure that the auxiliary cleaning assembly 8 drives the culture chamber 806 to move smoothly and accurately between different areas. The water pump 701 can also control the flow and pressure of water pumping and water conveying to ensure that the flushing assembly 7 has stable and efficient flushing effect on the culture chamber 806, and further improve the use effect.

[0036] Specifically, as shown in Figure 2 The second cleaning pool 4 is internally connected with a moving filter screen frame 16, and the water suction end of the water pump 701 is located below the moving filter screen frame 16.

[0037] Specifically, as shown in Figure 5 The frame 2 is internally fixedly connected with a guide slide rod 17, and the mounting plate 12 is slidably connected to the surface of the guide slide rod 17.

[0038] In the embodiment, when the flushing assembly 7 works, the cell fragments, impurity particles, and other pollutants washed down by the high-pressure flushing nozzle 703 will enter the flushing liquid in the second cleaning pool 4. The moving filter screen frame 16 can intercept these larger impurity particles to ensure the cleanliness of the flushing liquid. The guide slide rod 17 can ensure that the mounting plate 12 moves smoothly along the predetermined straight line direction, avoiding shaking or deviation of the mounting plate 12 during movement. This not only ensures the stability of the overall movement of the auxiliary cleaning assembly 8, but also makes the transfer of the culture chamber 806 between different areas more accurate.

[0039] Working principle: after the cell induction separation is completed, the culture chamber 806 will be left with cell fragments, inducers and other contaminants, when the culture chamber 806 needs to be cleaned, first start the electric screw rod 10 through the integrated controller 15, the electric screw rod 10 will drive the moving block 11 to move left, the moving block 11 will drive the mounting plate 12 and the culture chamber 806 to move together, then move the culture chamber 806 to the upper side of the second cleaning tank 4, then make the air cylinder 13 drive the culture chamber 806 to move down until the culture chamber 806 is immersed in the second cleaning tank 4, then start the water pump 701, the water pump 701 will extract the cleaning liquid from the second cleaning tank 4, then evenly distribute the flushing liquid to the two side pipes 702 through the metal shunt pipe 704, and then spray the flushing liquid to the culture chamber 806 through the plurality of inclined high-pressure flushing nozzles 703, during the flushing process, the culture chamber 806 can be rotated under the drive of the motor 804, so that the flushing is more uniform, that is, the surface of the culture chamber 806 can be flushed at multiple angles, so that the cell fragments and inducers and other contaminants in the culture chamber 806 will be washed off, the washed cell fragments and inducers will fall to the top of the movable filter screen, the movable filter screen frame 16 will intercept them to prevent them from falling into the cleaning liquid, after the flushing is completed, the air cylinder 13 drives the culture chamber 806 to rise from the second cleaning tank 4, then the electric screw rod 10 drives the culture chamber 806 to move to the upper side of the first cleaning tank 3, the air cylinder 13 drives the culture chamber 806 to immerse in the first cleaning tank 3 again, start the ultrasonic wave generating device 6 to generate ultrasonic waves, and use the cavitation effect and mechanical vibration of the ultrasonic waves to further clean the culture chamber 806 and remove stubborn stains, that is, the culture chamber 806 can be cleaned comprehensively, after the cleaning is completed, the electric screw rod 10 drives the cleaned culture chamber 806 to move to the upper side of the operation table 5.

[0040] It should be noted that the specific structure, working principle and use method of the ultrasonic wave generating device and the high-pressure flushing nozzle involved in the present application are all prior art, therefore, no more details are added in the text.

[0041] The above is only a preferred embodiment of the present application, and is not used to limit the present application, any modification, equivalent replacement and improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A cell induction and separation device, comprising a base (1), characterized in that: The top of the base (1) is fixedly connected to a frame (2), and the frame (2) is provided with a first cleaning pool (3), a second cleaning pool (4) and an operating table (5) from left to right. The first cleaning pool (3) is provided with an ultrasonic generator (6), the second cleaning pool (4) is provided with a rinsing assembly (7), and the frame (2) is provided with an auxiliary cleaning assembly (8). The auxiliary cleaning assembly (8) includes a support plate (801) and a connecting shaft (802). The rinsing assembly (7) includes a water pump (701), which is fixedly connected to the rear side of the frame (2) and the suction end of the water pump (701) extends into the interior of the second cleaning tank (4). Both sides of the interior of the second cleaning tank (4) are fixedly connected to conduits (702), and the side of the two conduits (702) that are close to each other is fixedly connected to a high-pressure flushing nozzle (703). The number of high-pressure flushing nozzles (703) is set to multiple and they are arranged at an angle. The outlet end of the water pump (701) is fixedly connected to a metal diverter pipe (704), which passes through the frame (2) and is fixedly connected to the conduits (702).

2. The cell induction and separation device according to claim 1, characterized in that: The top end of the connecting shaft (802) is rotatably connected to the inside of the support plate (801). A U-shaped frame (803) is fixedly connected to the top of the support plate (801). A drive motor (804) is fixedly connected inside the U-shaped frame (803). The output end of the drive motor (804) passes through the support plate (801) and is fixedly connected to the top end of the connecting shaft (802).

3. The cell induction and separation device according to claim 1, characterized in that: A connecting frame (805) is fixedly connected to the bottom surface of the connecting shaft (802). The number of connecting frames (805) is set to several and they are arranged in a ring. A culture chamber (806) is fixedly connected to the top of the connecting frame (805).

4. The cell induction and separation device according to claim 2, characterized in that: A groove (9) is provided on the rear side of the inner wall of the frame (2). An electric lead screw (10) is rotatably connected inside the groove (9). A moving block (11) is threadedly connected to the surface of the electric lead screw (10). An mounting plate (12) is fixedly connected to the front side of the moving block (11). A cylinder (13) is fixedly connected to the top of the mounting plate (12). The telescopic end of the cylinder (13) passes through the mounting plate (12) and is fixedly connected to the top of the U-shaped frame (803).

5. The cell induction and separation device according to claim 1, characterized in that: A drain pipe (14) is fixedly connected to the left side of the first cleaning pool (3), and the drain pipe (14) passes through the frame (2) and extends to the outside of the frame (2).

6. The cell induction and separation device according to claim 4, characterized in that: An integrated controller (15) is provided on the front side of the operating table (5), and the ultrasonic generator (6), electric lead screw (10) and water pump (701) are all electrically connected to the integrated controller (15).

7. The cell induction and separation device according to claim 1, characterized in that: The second cleaning tank (4) has a movable filter frame (16) slidably connected inside, and the water pump (701) suction end is located below the movable filter frame (16).

8. The cell induction and separation device according to claim 4, characterized in that: The frame (2) is fixedly connected to a guide slide rod (17), and the mounting plate (12) is slidably connected to the surface of the guide slide rod (17).

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