Microfluidic substrate and microfluidic chip

Abstract

The utility model provides a micro-fluidic substrate and a micro-fluidic chip, and relates to the technical field of analysis and detection, according to the micro-fluidic substrate, the processing and assembling links of independent parts are eliminated through an integrally formed structure, and the manufacturing cost is reduced; meanwhile, the integrated structure does not deform in the puncturing process, so that the accuracy and reliability of the puncturing action are ensured, and the defects of high cost and poor puncturing effect in a traditional micro-fluidic chip are overcome; the micro-fluidic chip comprises a micro-fluidic substrate, a water box and a sealing film, the water box is arranged in a water box placement area, the sealing film is arranged on the micro-fluidic substrate, and the sealing film serves as a flexible sealing layer covering the micro-fluidic substrate to prevent fluid in a fluid groove in a substrate body from leaking. Compared with a microfluidic cover plate which is in ultrasonic welding with a microfluidic substrate in the prior art, the cover sealing film is lower in manufacturing cost.

Description

Technical Field

[0001] This utility model relates to the field of analytical testing technology, and in particular to a microfluidic substrate and a microfluidic chip. Background Technology

[0002] Microfluidics has enormous potential in fields such as biology, chemistry, and medicine, and has developed into a new interdisciplinary research area encompassing biology, chemistry, medicine, fluid dynamics, electronics, materials science, and mechanics. Centrifugal microfluidic chips, which drive and control fluid flow in microchannels using centrifugal force, offer advantages such as high integration, automation, miniaturization, and parallel detection of multiple samples or indicators, and have become an important branch of the microfluidic chip technology field.

[0003] Traditional microfluidic chips include two main types. One type comprises a microfluidic substrate and a microfluidic cover plate. The microfluidic substrate contains a water tank, while the microfluidic cover plate has a piercing mechanism. The microfluidic cover plate is fixed to the microfluidic substrate by ultrasonic welding, thereby enabling the piercing mechanism to pierce the water tank. This method increases the manufacturing cost of the microfluidic chip. Another type of microfluidic chip includes a microfluidic substrate, a piercing mechanism, and a sealing membrane. The microfluidic substrate also contains a water tank, and the piercing mechanism is a separate component located above the microfluidic substrate and fixed by the sealing membrane. When the water tank moves upward, the piercing mechanism, fixed by the sealing membrane, pierces the sealing membrane of the water tank. However, because the sealing membrane is made of a flexible material, the sealing membrane deforms during the piercing process, which can easily affect the piercing efficiency of the sealing membrane of the water tank.

[0004] Therefore, how to reduce the manufacturing cost of microfluidic chips while improving the puncture efficiency of the sealing membrane of the water box has become a technical problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, one of the objectives of this utility model is to provide a microfluidic substrate to solve the technical problems of high manufacturing cost of microfluidic chips or low efficiency in puncturing the sealing film of water boxes in the prior art.

[0006] The second objective of this invention is to provide a microfluidic chip containing a microfluidic substrate.

[0007] To achieve one of the above objectives, this utility model provides a microfluidic substrate, including a substrate body, a water box placement area inside the substrate body, a puncture mechanism on the water box placement area, the puncture mechanism being integrally formed with the substrate body, the puncture mechanism puncturing the sealing film of the water box placed in the water box placement area, thereby allowing the fluid in the water box to be transferred to the fluid channel of the substrate body under the action of centrifugal force.

[0008] Optionally, the piercing mechanism includes a piercing lug and a piercing needle. The piercing lug is integrally formed with the substrate body, and the piercing needle is located below the piercing lug and is integrally formed with the piercing lug. The piercing lug is also provided with a liquid-spinning hole for transferring the fluid of the water box to the fluid groove inside the substrate body.

[0009] Optionally, the puncture is performed on the edge of the sealing membrane of the water tank.

[0010] Optionally, the lower surface of the piercing needle is a beveled surface, with its inclination angle tilting from the distal end to the proximal end of the piercing lug.

[0011] Optionally, the oblique cut surface is bent or arc-shaped.

[0012] Optionally, the piercing needle forms at least a portion of the sidewall of the liquid ejection orifice.

[0013] Optionally, the puncture lug is provided with a microchannel, which is simultaneously connected to the fluid groove of the substrate body and the liquid ejection hole.

[0014] Optionally, the lower surface of the piercing lug is provided with at least one piercing needle, the piercing needles are arranged at preset distances, and the number of liquid ejection holes is the same as the number of piercing needles; the piercing mechanism includes at least one, which is arranged at preset distances above the side wall of the water box placement area.

[0015] Optionally, it also includes a connecting portion, which is simultaneously fixedly connected to both the piercing mechanism and the substrate body.

[0016] To achieve the second objective mentioned above, this utility model provides a microfluidic chip, including any of the microfluidic substrates described above, as well as a water box and a sealing film. The microfluidic substrate includes at least one piece, the water box is disposed in the water box placement area, and the sealing film is disposed on the microfluidic substrate.

[0017] The microfluidic substrate provided by this utility model has the following technical effects:

[0018] This microfluidic substrate includes a substrate body with a water tank placement area inside. A puncture mechanism is integrally formed with the substrate body. The puncture mechanism punctures the sealing membrane of the water tank placed in the water tank placement area, allowing fluid in the water tank to transfer to the fluid channel of the substrate body. This invention eliminates the processing and assembly of independent components through its integral molding structure, reducing manufacturing costs. Simultaneously, the integral structure is less prone to deformation during puncture, ensuring the accuracy and reliability of the puncture action, overcoming the shortcomings of high cost and poor puncture effect in traditional microfluidic chips.

[0019] The microfluidic chip provided by this utility model has the following technical effects:

[0020] This type of microfluidic chip includes a microfluidic substrate, a water tank, and a capping film. The microfluidic substrate includes at least one piece, the water tank is disposed in the water tank placement area, and the capping film is disposed on the microfluidic substrate. The capping film serves as a flexible sealing layer covering the microfluidic substrate to prevent fluid leakage in the fluid grooves on the substrate body. Compared with the existing microfluidic cover plate ultrasonically welded to the microfluidic substrate, the capping film has a lower manufacturing cost. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a three-dimensional structural schematic diagram of a preferred embodiment of the microfluidic substrate of this utility model;

[0023] Figure 2 yes Figure 1 Top view of the microfluidic substrate;

[0024] Figure 3 yes Figure 1 Bottom view of the microfluidic substrate;

[0025] Figure 4 yes Figure 1 Another perspective three-dimensional structural diagram of the microfluidic substrate;

[0026] Figure 5 yes Figure 4 Enlarged structural diagram at point A;

[0027] Figure 6 yes Figure 1 A three-dimensional structural schematic diagram of another preferred embodiment of the microfluidic substrate;

[0028] Figure 7 This is a schematic diagram of a preferred embodiment of the microfluidic chip of this utility model.

[0029] in, Figures 1-7 :

[0030] 1. Substrate body; 11. Water tank placement area; 12. Puncture mechanism; 121. Puncture lug; 1211. Spray hole; 1212. Microchannel; 122. Puncture needle; 1221. Beveled surface; 13. Fluid tank; 14. Connecting part;

[0031] 10. Microfluidic substrate; 20. Water box; 30. Sealing film. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other implementation methods obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0033] In existing technologies, microfluidic chip technology achieves detection functions by driving fluids with centrifugal force. However, traditional structures suffer from high costs and low puncture efficiency. For example, using ultrasonic welding to fix the cover plate with the puncture mechanism 12 to the substrate results in complex processes and increased costs. Furthermore, the separately designed puncture mechanism 12, fixed by a flexible sealing film, is prone to deformation during the puncture process, affecting the puncture effect. These shortcomings limit the widespread application of microfluidic chips, especially in scenarios requiring high-precision puncture and low-cost manufacturing.

[0034] To address the aforementioned issues, a technical solution that simplifies the structure while improving stability needs to be explored. In traditional methods, the piercing mechanism 12, either as a separate component or reliant on external connections, leads to increased costs and decreased reliability. Integrating the piercing function directly into the substrate body 1 avoids the assembly processes and material consumption associated with additional components, while also reducing positioning deviations caused by the deformation of flexible materials during the piercing process. Therefore, designing the piercing mechanism 12 and the substrate body 1 as a single, integrally formed structure becomes the key approach to solving these problems.

[0035] Therefore, as Figures 1-6 As shown, this utility model proposes a microfluidic substrate, including a substrate body 1, a water box placement area 11 is provided on the inner side of the substrate body 1, and a puncture mechanism 12 is provided on the water box placement area 11. The puncture mechanism 12 is integrally formed with the substrate body 1. The puncture mechanism 12 punctures the sealing film of the water box placed in the water box placement area 11, so that the fluid in the water box 20 can be transferred to the fluid groove 13 of the substrate body 1 under the action of centrifugal force.

[0036] It should be noted that the substrate body 1 and the piercing mechanism 12 of this utility model are made of the same material, namely, hard material, such as polycarbonate or polymethyl methacrylate.

[0037] The substrate body 1 is the main structure supporting the microchannels 1212 and functional areas. It can be manufactured using injection molding, for example, using polycarbonate or polymethyl methacrylate materials, to achieve a high-precision structure and reduce costs. The shape of the substrate body 1 can be fan-shaped, semi-circular, or circular; in this embodiment, a fan-shaped shape is preferred. When the substrate body 1 is fan-shaped, the inner and outer sides are shown in the diagram. Figure 2 As shown.

[0038] The water box placement area 11 is a specific area used to position and fix the water box. Specifically, it can be achieved by setting a limiting structure on the substrate body 1. The height of the water box placement area 11 is greater than the thickness of the water box, so that the water box can move up and down in the water box placement area 11 to complete the puncture of the sealing film of the water box.

[0039] The piercing mechanism 12 and the substrate body 1 are integrally formed by the same processing steps to form an inseparable whole structure. Specifically, the piercing lug 121 and the piercing needle 122 can be directly formed during mold forming.

[0040] The puncturing mechanism 12 punctures the sealing film to form a hole, and the fluid in the water box is introduced into the fluid tank 13 of the substrate body 1 through the liquid ejection hole 1211 and the microchannel 1212 under the action of centrifugal force.

[0041] Specifically, when the water box is placed in the water box placement area 11, the piercing mechanism 12, as an integrally formed structure, is located above the water box. When the microfluidic chip equipped with the piercing mechanism 12 is placed on the centrifuge tray, the corresponding mechanism within the tray can lift the water box 20 upwards, thereby causing the piercing mechanism 12 to press against the sealing membrane of the water box and pierce the sealing membrane as the water box 20 rises. During the piercing process, the piercing needle 122 penetrates the edge of the sealing membrane to form a rupture. During centrifugation, the fluid in the water box is guided into the fluid groove 13 of the substrate body 1 through the liquid ejection hole 1211 and the microchannel 1212. Since the piercing mechanism 12 and the substrate body 1 are an integral structure, no additional fixing components are required, and stable mechanical strength can be maintained during piercing, avoiding displacement or incomplete piercing caused by flexible support.

[0042] Compared to existing technologies, traditional solutions require the puncture mechanism 12 to be manufactured separately and fixed by welding or a flexible membrane, leading to material waste and increased process complexity, thus increasing costs. This invention, however, eliminates the processing and assembly of independent components through a one-piece molded structure, reducing manufacturing costs. Simultaneously, the one-piece structure does not deform during the puncture process, ensuring the accuracy and reliability of the puncture action. In short, this invention not only simplifies the manufacturing process to reduce production costs but also improves the stability and efficiency of the puncture action through one-piece molding support, overcoming the shortcomings of high cost and poor puncture effect in traditional microfluidic chips.

[0043] For details, please refer to [link / reference]. Figures 1-5 As shown, the piercing mechanism 12 includes a piercing lug 121 and a piercing needle 122. The piercing lug 121 is a plate-shaped structure with a certain thickness. The piercing lug 121 is integrally formed with the substrate body 1. The piercing needle 122 is located below the piercing lug 121 and is also integrally formed with the piercing lug 121. The piercing lug 121 is also provided with a liquid-spinning hole 1211 for transferring the fluid of the water box to the fluid groove 13 inside the substrate body 1.

[0044] The piercing lug 121 is a supporting structure connected to the upper side wall of the water tank placement area 11. It can be integrally formed with the substrate body 1 through injection molding or micromachining technology, providing a fixed base for the piercing needle 122. The piercing needle 122 is a sharp component extending below the piercing lug 121, which can be manufactured simultaneously with the piercing lug 121 using precision molding technology. Its function is to pierce the sealing membrane of the water tank through mechanical penetration. The liquid ejection hole 1211 is a fluid channel penetrating the piercing lug 121. Its shape matches the shape of the piercing needle 122, and its principle is to use centrifugal force to guide the liquid released from the water tank to the fluid groove 13 of the substrate body 1.

[0045] Specifically, when the water box is placed in the water box placement area 11, the water box 20 is pushed upward by the corresponding mechanism of the external centrifugal tray, and the piercing needle 122 directly contacts the edge area of ​​the water box sealing film. Since the piercing lug 121 and the substrate body 1 are an integral structure, the vertical displacement of the piercing needle 122 is constrained, allowing the piercing needle 122 to stably penetrate the sealing film. Simultaneously, the liquid ejection hole 1211 provided inside the piercing lug 121 forms a path connecting the water box and the fluid tank 13, and the liquid flows into the fluid tank 13 through the liquid ejection hole 1211 under the action of centrifugal force.

[0046] It should be noted that, to prevent residual liquid in the water tank, the piercing needle 122 punctures the edge of the sealing membrane. When the water tank is placed in the water tank placement area 11, the piercing needle 122 directly contacts the edge area of ​​the sealing membrane and applies pressure, penetrating the sealing membrane through its sharp end to form an opening. Compared to piercing the non-edge area of ​​the sealing membrane, the opening formed after piercing the edge makes it easier for liquid to be ejected. For example, when piercing the non-edge area, the sealing membrane in the edge area blocks the ejected liquid, making it easier for liquid residue to remain in the water tank.

[0047] As a preferred embodiment, such as Figure 4 and Figure 5As shown, the lower surface of the puncture needle 122 is preferably a beveled surface 1221, with its inclination angle tilting from the distal end to the proximal end of the puncture lug 121. That is, the thickness of the puncture needle 122 continuously decreases from the distal end to the proximal end of the puncture lug 121. The beveled surface 1221 is a non-planar structure on the lower surface of the puncture needle 122. This structure can reduce the contact area between the needle tip and the sealing film during the puncture process, thereby reducing the puncture resistance. On the other hand, the puncture needle 122 can also generate a pulling tendency on the water box.

[0048] Specifically, when the water box 20 is placed in the water box placement area 11, the water box 20 is pushed upward by the corresponding mechanism of the external centrifuge tray and moves towards the piercing needle 122. At this time, the long end of the inclined direction of the piercing needle 122's beveled surface 1221 first contacts the sealing membrane (close to the edge of the sealing membrane, but not in contact with the edge of the sealing membrane). The beveled surface 1221 contacts the water box wall in the edge area of ​​the sealing membrane. As the water box 20 continues to move upward, the wall of the water box 20 slides along the beveled surface 1221, causing the water box to move slightly within the chip plane, so that the piercing needle 122 pierces the sealing membrane downward while opening the sealing membrane opening, until the beveled surface 1221 slides completely into the water box. In this embodiment, the tip of the piercing needle 122 penetrates the sealing film to form an opening. The beveled surface 1221 interacts with the side wall of the water tank 20 to ensure the opening is tightly against the water tank wall. Compared to piercing the non-edge area of ​​the sealing film of the water tank with the piercing needle 122, the opening formed after piercing at the edge makes it easier to eject liquid outward. For example, when piercing in a non-edge area, the sealing film in the edge area blocks the ejected liquid, causing liquid residue to easily remain in the water tank. During this process, the low resistance of the beveled surface 1221 reduces the driving force required for piercing, and the tilt direction also ensures that the cutting path matches the ejection hole 1211, allowing the fluid in the water tank to directly enter the fluid tank 13 of the substrate body 1 through the ejection hole 1211.

[0049] In the above embodiment, the oblique surface 1221 is an inclined contact surface formed on the lower surface of the piercing needle 122. Its function is to guide the piercing action and disperse the resistance during piercing. The oblique surface 1221 in this embodiment can be bent, arc-shaped, or other shapes. As long as it can achieve the purpose of dispersing the resistance during piercing, it is within the protection scope of this utility model.

[0050] In the above embodiment, the puncture needle 122 constitutes at least part of the sidewall of the liquid ejection hole 1211. Specifically, the main structure of the puncture needle 122 directly forms part of the inner wall structure of the liquid ejection hole 1211, that is, the thickness of part of the sidewall of the liquid ejection hole 1211 is greater than the thickness of the remaining sidewall, which serves to reduce the risk of leakage during the fluid transfer process.

[0051] Specifically, the side of the piercing needle 122 forms a portion of the sidewall of a liquid-throwing hole 1211 below the piercing lug 121. When the piercing needle 122 penetrates the sealing membrane of the water tank, the liquid-throwing hole 1211 directly communicates with the interior of the water tank. After being thrown out of the water tank, the fluid enters the liquid-throwing hole 1211 along the channel formed by the sidewall of the piercing needle 122, and then transfers to the fluid tank 13 of the substrate body 1. Since the sidewall of the piercing needle 122 and the liquid-throwing hole 1211 partially overlap, the fluid can be transferred without going through an additional path. At the same time, the integral structure of the piercing needle 122 can maintain the shape stability of the liquid-throwing hole 1211.

[0052] As a preferred embodiment, such as Figure 1 and Figure 2 As shown, the puncture lug 121 is provided with a microchannel 1212, which is connected to the fluid groove 13 and the liquid ejection hole 1211 of the substrate body 1.

[0053] The microchannel 1212 is a tiny channel simultaneously provided on the substrate body 1 and the puncture lug 121, which can be implemented by injection molding, and is used to establish a communication path between the fluid groove 13 and the liquid ejection hole 1211. The fluid groove 13 is a groove structure inside the substrate body 1 used to contain fluid, and is used to store or guide the flow of fluid.

[0054] When the puncturing needle 122 punctures the sealing membrane of the water tank, fluid enters the microchannel 1212 through the dispensing hole 1211, and then flows directionally along the microchannel 1212 to the fluid tank 13. Since the microchannel 1212 is directly integrated into the puncturing lug 121, the fluid transfer path is confined within the integrated structure of the puncturing mechanism 12 and the substrate body 1. Through the guiding effect of the microchannel 1212, the fluid can be stably transferred from the dispensing hole 1211 to the fluid tank 13, avoiding leakage or dispersion during the transfer process.

[0055] In a more preferred embodiment, the lower surface of the same puncture lug 121 may also be provided with a plurality of puncture needles 122, such as two or three, with the puncture needles 122 spaced at a preset distance, and the number of liquid ejection holes 1211 being the same as the number of puncture needles 122.

[0056] The preset interval is a fixed distance between multiple puncture needles 122, which can be achieved by equidistant arrangement. This design ensures that the puncture action is evenly distributed, avoiding local stress concentration that could lead to incomplete damage to the sealing membrane. The number of liquid ejection holes 1211 corresponds one-to-one with the number of puncture needles 122, so that the fluid generated by each puncture action can be directionally transferred to the fluid tank 13 through the corresponding hole.

[0057] Specifically, as the water tank is placed in the water tank placement area 11 and moves upward, multiple piercing needles 122 on the lower surface of the piercing lug 121 simultaneously contact the water tank sealing membrane. Because the piercing needles 122 are spaced at preset intervals, multiple piercing points are evenly distributed along the edge of the sealing membrane. During the piercing process, the sealing membrane is torn apart by the combined action of multiple needles, forming a regular opening. The corresponding liquid-discharging hole 1211 for each piercing needle directly guides the released fluid into the fluid channel 13 of the substrate body 1, avoiding fluid stagnation caused by a single channel. The consistent number of piercing needles 122 and liquid-discharging holes 1211 further ensures that each piercing area has an independent fluid guidance path, thereby improving fluid transfer efficiency.

[0058] In a more preferred embodiment, a plurality of puncture mechanisms 12 may be included, which are disposed at a predetermined distance above the side wall of the water box placement area 11.

[0059] The piercing mechanism 12 is formed by molding the same mold as the substrate body 1 or by injection molding in one step. The preset distance is the center-to-center distance between adjacent piercing mechanisms 12, which can be achieved by uniform distribution or asymmetrical arrangement to ensure that the piercing mechanisms 12 form a multi-point piercing layout above the side wall of the water box placement area 11.

[0060] In some specific embodiments, two or four piercing mechanisms 12 may be provided above the side wall of the water box placement area 11, and the spacing between adjacent piercing mechanisms 12 may be 8 mm or adjusted according to the size of the water box. For example, when the length of the water box is 30 mm, three piercing mechanisms 12 are provided above the side wall of the water box placement area 11 with a spacing of 10 mm to ensure that the piercing force is evenly distributed.

[0061] As a more preferred implementation method, such as Figure 6 As shown, it also includes a connecting part 14, which is fixedly connected to both the piercing mechanism 12 and the substrate body 1. The fixed connection is preferably an integrally formed structure. The connecting part 14 can be a rod-shaped structure or a plate-shaped structure. The specific shape of the connecting part is not limited, as long as it can achieve the purpose of connecting the piercing mechanism 12 and the substrate body 1.

[0062] When the water box 20 moves toward the puncture mechanism 12, the puncture mechanism 12 can exert a force on the water box sealing film, that is, the puncture mechanism 12 can puncture the water box sealing film. For this reason, the puncture mechanism 12 generates a corresponding reaction force. In order to prevent the reaction force from causing the puncture mechanism 12 to deform, a connecting part 14 is provided between the substrate body 1 and the puncture mechanism 12 in this embodiment. The connecting part 14 is also located above the water box placement area 11.

[0063] In this embodiment, a connecting part 14 is added between the piercing mechanism 12 and the substrate body 1. By strengthening the connection between the piercing mechanism 12 and the substrate body, the deformation of the piercing mechanism 12 during the piercing process is further reduced. High-strength or low-strength materials can be selected while meeting the piercing requirements, and the range of material selection can be increased, which can further reduce costs.

[0064] In detail, the connecting part 14 is preferably a connecting rod, one end of which is provided on both sides of the piercing lug, and the other end of which is provided on the substrate body. In this embodiment, the connecting rod is preferably integrally formed with the piercing lug and the basic body.

[0065] It should be noted that the shape of the connecting rod is preferably consistent with the shape of the inner wall of the water box placement area 11, that is, it is preferably an arc-shaped connecting rod, which pierces the outer edge of the lug 121 to form part of the middle structure of the arc-shaped connecting rod.

[0066] This invention also provides a microfluidic chip, such as Figure 7 As shown, it includes a microfluidic substrate 10, a water tank 20, and a sealing film 30. The microfluidic substrate 10 includes at least one piece, the water tank 20 is disposed in the water tank placement area 11, and the sealing film 30 is disposed on the microfluidic substrate 10.

[0067] The microfluidic substrate 10 is an integral structure including the substrate body 1, the water box placement area 11, and the puncture mechanism 12. The puncture mechanism 12 is integrally formed with the substrate body 1, which can be achieved by injection molding. The integrated design reduces the number of independent components. For example, the microfluidic substrate 10 is preferably fan-shaped, and multiple microfluidic substrates 10 are spliced ​​together to achieve a circle, so as to meet the integration requirements of different detection scenarios.

[0068] The sealing film 30 is a flexible sealing layer covering the microfluidic substrate 10. It can be implemented by heat sealing film or pressure-sensitive adhesive film to prevent fluid leakage in the fluid groove 13 on the substrate body 1. Compared with the microfluidic cover plate ultrasonically welded to the microfluidic substrate 10 in the prior art, the sealing film 30 has a lower manufacturing cost.

[0069] Compared with existing technologies where the puncture mechanism is ultrasonically welded to the microfluidic cover plate or exists as an independent component, this invention reduces the manufacturing cost of the microfluidic chip and improves the puncture efficiency of the puncture mechanism on the sealing membrane of the water box 20, ensuring the reliability and consistency of the fluid transfer process.

[0070] In the description of this utility model, it should be noted that, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0071] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0072] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A microfluidic substrate, comprising a substrate body, characterized in that, The substrate body has a water box placement area on its inner side, and a puncture mechanism is provided on the water box placement area. The puncture mechanism is integrally formed with the substrate body. The puncture mechanism punctures the sealing film of the water box placed in the water box placement area, so that the fluid in the water box can be transferred to the fluid tank of the substrate body under the action of centrifugal force.

2. The microfluidic substrate according to claim 1, characterized in that, The piercing mechanism includes a piercing lug and a piercing needle. The piercing lug is integrally formed with the substrate body. The piercing needle is located below the piercing lug and is integrally formed with the piercing lug. The piercing lug is also provided with a liquid-spinning hole for transferring the fluid of the water box to the fluid groove inside the substrate body.

3. The microfluidic substrate according to claim 2, characterized in that, The puncture is performed on the edge of the sealing membrane of the water box.

4. The microfluidic substrate according to claim 2, characterized in that, The lower surface of the piercing needle is a beveled surface, and its inclination angle is inclined from the distal end to the proximal end of the piercing lug.

5. The microfluidic substrate according to claim 4, characterized in that, The oblique cut surface is bent or arc-shaped.

6. The microfluidic substrate according to claim 2, characterized in that, The piercing needle forms at least a portion of the sidewall of the liquid ejection orifice.

7. The microfluidic substrate according to claim 2, characterized in that, The puncture lug is provided with a microchannel, which is simultaneously connected to the fluid groove of the substrate body and the liquid ejection hole.

8. The microfluidic substrate according to any one of claims 2-7, characterized in that, The lower surface of the piercing lug is provided with at least one piercing needle, the piercing needles are arranged at preset distances, and the number of liquid ejection holes is the same as the number of piercing needles; The puncture mechanism includes at least one, which is located at a predetermined distance above the side wall of the water box placement area.

9. The microfluidic substrate according to claim 1, characterized in that, It also includes a connecting part, which is fixedly connected to both the piercing mechanism and the substrate body.

10. A microfluidic chip, characterized in that, The microfluidic substrate according to any one of claims 1-9 further includes a water box and a sealing film. The microfluidic substrate includes at least one piece, the water box is disposed in the water box placement area, and the sealing film is disposed on the microfluidic substrate.

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