A quantitative microfluidic reagent card

Abstract

This utility model discloses a quantitative microfluidic reagent card, relating to the field of microfluidic reagent card technology. It includes a plate and a quantitative component. The plate has microchannels inside, with vent holes at the ends of the microchannels. The quantitative component is located in the center of the plate and includes a rotating shaft, a quantitative cylinder, a flared ring, an anti-slip sleeve, and a through-hole. The rotating shaft is rotatably connected to the middle of the plate, and the quantitative cylinder is fixed to the top of the rotating shaft. Through the quantitative component, before use, rotating the anti-slip sleeve rotates the quantitative cylinder, misaligning the through-hole with the ends of the microchannels. When the sample is dropped into the quantitative cylinder, the flared ring widens the opening, guiding the sample droplet and facilitating accurate entry into the quantitative cylinder, preventing spillage and sample volume deviation. After dropping, rotating the quantitative cylinder with the anti-slip sleeve aligns the through-hole with the ends of all microchannels simultaneously.

Description

Technical Field

[0001] This utility model relates to the field of microfluidic reagent card technology, specifically a quantitative microfluidic reagent card. Background Technology

[0002] Microfluidic reagent cards are portable detection devices that integrate microfluidic technology. They are usually in the form of plates or chips and achieve sample processing, biochemical reactions, and detection analysis by precisely manipulating trace amounts of fluid within a microchannel network.

[0003] For example, utility model publication CN222212782U discloses a microfluidic reagent card. This utility model sets up multiple microchannels connected to the same injection hole. Under the capillary action of the microchannels, the test sample can flow sequentially through the marking area, test area and quality control area within the microchannel, autonomously completing the detection of multiple analytes. It has high detection efficiency and simple operation. However, in actual use, this type of microfluidic reagent card requires 50 μL of the test sample to be dripped into the injection hole. Due to the small opening, the sample is prone to deflection when dripping into the injection hole, and it is difficult for the freshly dripped sample to contact the ends of multiple microchannels at the same time, making it inconvenient to keep the sample volume in each microchannel the same. Utility Model Content

[0004] The purpose of this invention is to provide a quantitative microfluidic reagent card to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a quantitative microfluidic reagent card, comprising a plate and a quantitative component. The plate has microchannels inside, and the ends of the microchannels are provided with vent holes. The quantitative component is located in the center of the plate and includes a rotating shaft, a quantitative cylinder, a flared ring, an anti-slip sleeve, and a through hole. The rotating shaft is rotatably connected to the middle of the plate, and the quantitative cylinder is fixed to the top of the rotating shaft. The top of the quantitative cylinder is provided with a flared ring, and the outer side of the flared ring is fitted with an anti-slip sleeve. The lower outer side of the quantitative cylinder is provided with a through hole.

[0006] Furthermore, the microchannels are serpentine in shape and are equidistantly distributed circumferentially with respect to the interior of the plate.

[0007] Furthermore, a limiting block is fixed on the outer circumferential surface of the bottom of the rotating shaft, a limiting groove is opened at the bottom of the plate, and the limiting block is slidably connected to the plate through the limiting groove.

[0008] Furthermore, a striking assembly is provided on the upper outer side of the metering cylinder, and the striking assembly includes a support plate, a sliding rod and a spring. The upper end of the support plate is slidably connected to the sliding rod, and the outer side of the sliding rod is sleeved with a spring.

[0009] Furthermore, the spring abuts against the support plate, and the support plate is fixedly connected to the plate body.

[0010] Furthermore, the striking assembly also includes a hammer head and a toothed block, with one end of the spring connected to the hammer head and the other end of the hammer head slidably connected to the toothed block.

[0011] Furthermore, the hammer head is fixedly connected to the slide rod, and the hammer head is wedge-shaped.

[0012] Furthermore, the toothed blocks are equidistantly distributed circumferentially about the outer side of the metering cylinder, and the toothed blocks are fixedly connected to the metering cylinder.

[0013] This invention provides a quantitative microfluidic reagent card with the following advantages:

[0014] 1. This utility model, through the setting of the quantitative component, allows the quantitative cylinder to rotate by rotating the anti-slip sleeve before use, so that the through hole and the end of the microchannel are misaligned. When the sample is dripped into the quantitative cylinder, the flaring ring will widen the opening position to guide the sample droplet, thereby facilitating the accurate entry of the sample droplet into the quantitative cylinder and avoiding spillage that could cause deviation in sample quantity. After dripping, rotating the quantitative cylinder with the anti-slip sleeve can simultaneously align the through hole with the ends of all microchannels, thereby allowing the liquid to flow simultaneously and ensuring that a quantitative amount of sample solution flows into all microchannels, improving the accuracy of subsequent detection processes.

[0015] 2. This utility model, through the setting of the striking component, causes the metering cylinder to move along with the toothed block during rotation. This allows the inclined surface of the toothed block to press the hammer head, which in turn compresses the spring under the guidance of the inclined surface, storing energy. When the toothed block separates from the hammer head, the spring pushes the hammer head to strike the side wall of the metering cylinder, causing the metering cylinder to vibrate. This vibrates the metering cylinder, dislodging air bubbles from the sample droplets and preventing them from affecting the flow of the sample solution. Furthermore, after releasing the pressure, the hammer head remains positioned between the toothed blocks, thus limiting the angle of the metering cylinder after rotation and enhancing the stability after adjustment. Attached Figure Description

[0016] Figure 1 This is a frontal three-dimensional structural diagram of a quantitative microfluidic reagent card according to the present invention;

[0017] Figure 2 This is a bottom view schematic diagram of the overall structure of a quantitative microfluidic reagent card according to this utility model;

[0018] Figure 3 This is a three-dimensional structural diagram of the quantitative component of a quantitative microfluidic reagent card according to the present invention.

[0019] In the diagram: 1. Plate; 2. Microchannel; 3. Vent hole; 4. Metering component; 401. Rotating shaft; 402. Metering cylinder; 403. Flaring ring; 404. Anti-slip sleeve; 405. Through hole; 5. Limiting block; 6. Limiting groove; 7. Striking component; 701. Support plate; 702. Slide rod; 703. Spring; 704. Hammer head; 705. Tooth block. Detailed Implementation

[0020] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0021] like Figures 1 to 3 As shown, a quantitative microfluidic reagent card includes a plate body 1 and a quantitative component 4. The plate body 1 has microchannels 2 inside, and the ends of the microchannels 2 are provided with vent holes 3. The microchannels 2 are serpentine and are equidistantly distributed circumferentially around the interior of the plate body 1. The microchannels 2 have a marking area, a testing area, and a quality control area. Since this detection method is existing technology, it will not be described in detail in this application. The quantitative component 4 is located in the center of the plate body 1 and includes a rotating shaft 401, a quantitative cylinder 402, a flared ring 403, an anti-slip sleeve 404, and a through hole 405. The rotating shaft 401 is rotatably connected to the middle of the plate body 1, and the quantitative cylinder 402 is fixed to the top of the rotating shaft 401. The top of the quantitative cylinder 402 is provided with a flared ring 403. An anti-slip sleeve 404 is fitted on the outer side of 403 to increase the friction of the hand. A through hole 405 is opened on the lower outer side of the quantitative cylinder 402. When the through hole 405 is misaligned with the end of the microchannel 2, it can block the sample droplets to be tested. When the droplets inside are stable, the through hole 405 is aligned with the ends of all microchannels 2 at the same time, so that the liquid flows at the same time and a quantitative sample solution flows into all microchannels 2. A limiting block 5 is fixed on the outer peripheral surface of the bottom of the rotating shaft 401. A limiting groove 6 is opened at the bottom of the plate 1, and the limiting block 5 is slidably connected to the plate 1 through the limiting groove 6. When the limiting block 5 at the bottom of the rotating shaft 401 is in contact with the limiting groove 6, the through hole 405 can be aligned with the ends of all microchannels 2 at the same time, which is convenient for operation.

[0022] like Figure 1 and Figure 3As shown, a striking assembly 7 is provided on the upper outer side of the metering cylinder 402. The striking assembly 7 includes a support plate 701, a slide rod 702, and a spring 703. The slide rod 702 is slidably connected to the upper end of the support plate 701, and the spring 703 is sleeved on the outer side of the slide rod 702. The spring 703 abuts against the support plate 701, and the support plate 701 is fixedly connected to the plate body 1. The support plate 701 limits the end of the spring 703. The striking assembly 7 also includes a hammer head 704 and a toothed block 705. One end of the spring 703 is connected to the hammer head 704, and one end of the hammer head 704 is slidably connected to the toothed block. 705. The hammer head 704 is fixedly connected to the slide rod 702, and the hammer head 704 is wedge-shaped. When the hammer head 704 is squeezed by the toothed block 705, it will squeeze the spring 703 under the guidance of the inclined surface. The toothed blocks 705 are equidistantly distributed on the outer side of the metering cylinder 402, and the toothed blocks 705 are fixedly connected to the metering cylinder 402. When the metering cylinder 402 rotates, it will drive the toothed blocks 705 to move. When the toothed blocks 705 separate from the hammer head 704, the spring 703 will push the hammer head 704 to strike the side wall of the metering cylinder 402, which will make the metering cylinder 402 vibrate and shake out the air bubbles in the sample droplets.

[0023] In summary, when using this quantitative microfluidic reagent card, firstly according to... Figure 1 , Figure 2 and Figure 3 The structure shown involves rotating the anti-slip sleeve 404 to rotate the metering cylinder 402, causing the through hole 405 to misalign with the end of the microchannel 2, thus blocking it. Next, the sample is dripped into the metering cylinder 402. At this time, the flaring ring 403 widens the opening to guide the sample droplet. After dripping, rotating the metering cylinder 402 again using the anti-slip sleeve 404 also moves the toothed block 705, thereby using its inclined surface to press the hammer head 704. The hammer head 704, guided by the inclined surface, presses the spring 703 on the support plate 701 to store energy. When the toothed block 705 separates from the hammer head 704, the spring 703 will... Pushing the hammer 704 to strike the side wall of the quantitative cylinder 402 will cause the quantitative cylinder 402 to vibrate, which will dislodge air bubbles in the sample droplets and prevent them from affecting the flow of the sample solution. Then, when the limiting block 5 at the bottom of the rotating shaft 401 is in contact with the limiting groove 6, the through hole 405 can be aligned with the ends of all microchannels 2 at the same time, so that flow can be carried out simultaneously, allowing a quantitative amount of sample solution to flow into all microchannels 2, improving the accuracy of subsequent detection processes. Finally, after releasing the hammer, the hammer 704 can also be positioned between the toothed blocks 705, thereby limiting the angle of rotation of the quantitative cylinder 402 and enhancing the stability after adjustment.

[0024] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A quantitative microfluidic reagent card, comprising a plate (1) and a quantitative component (4), characterized in that, The plate (1) has a microchannel (2) inside, and the end of the microchannel (2) is provided with a vent hole (3). The metering component (4) is located in the center of the plate (1). The metering component (4) includes a rotating shaft (401), a metering cylinder (402), a flared ring (403), an anti-slip sleeve (404), and a through hole (405). The rotating shaft (401) is rotatably connected to the middle of the plate (1). The metering cylinder (402) is fixed at the top of the rotating shaft (401). The flared ring (403) is provided at the top of the metering cylinder (402). The anti-slip sleeve (404) is sleeved on the outside of the flared ring (403). The through hole (405) is provided on the lower outer side of the metering cylinder (402).

2. The quantitative microfluidic reagent card according to claim 1, characterized in that, The microchannel (2) is serpentine and is equidistantly distributed in a circular pattern with respect to the interior of the plate (1).

3. The quantitative microfluidic reagent card according to claim 1, characterized in that, A limiting block (5) is fixed on the bottom outer circumference of the rotating shaft (401), and a limiting groove (6) is opened at the bottom of the plate (1), and the limiting block (5) is slidably connected to the plate (1) through the limiting groove (6).

4. The quantitative microfluidic reagent card according to claim 1, characterized in that, The upper outer side of the metering cylinder (402) is provided with a striking component (7), and the striking component (7) includes a support plate (701), a slide rod (702) and a spring (703). The upper end of the support plate (701) is slidably connected to the slide rod (702), and the slide rod (702) is sleeved with a spring (703) on its outer side.

5. A quantitative microfluidic reagent card according to claim 4, characterized in that, The spring (703) abuts against the support plate (701), and the support plate (701) is fixedly connected to the plate body (1).

6. A quantitative microfluidic reagent card according to claim 4, characterized in that, The striking assembly (7) further includes a hammer (704) and a toothed block (705), one end of the spring (703) is connected to the hammer (704), and one end of the hammer (704) is slidably connected to the toothed block (705).

7. A quantitative microfluidic reagent card according to claim 6, characterized in that, The hammer head (704) is fixedly connected to the slide rod (702), and the hammer head (704) is wedge-shaped.

8. A quantitative microfluidic reagent card according to claim 6, characterized in that, The toothed blocks (705) are equidistantly distributed around the outer side of the metering cylinder (402), and the toothed blocks (705) are fixedly connected to the metering cylinder (402).

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