Silk bracket as well as preparation method and application thereof, and three-phase silk ligament graft and preparation method thereof

A technology of grafts and silk, which is applied in the field of medical tissue engineering and three-phase silk ligament grafts, can solve the problems of unsatisfactory mechanical properties of ligaments, small porosity of silk fibers, and poor bonding, etc., so as to improve biomechanical properties, The structure is simple and the effect of avoiding the concentration of stress

CN103505762BInactive Publication Date: 2015-06-10FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2015-06-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a silk ilk bracket as well as a preparation method and application thereof, and a three-phase silk ligament graft and a preparation method thereof. Firstly, silks are woven into a net-shaped bracket with a macroporous structure; then the net-shaped silk bracket is divided into three areas: A. a ligament area, B. a cartilage modification area and C. a bone modification area; ligament cells, chondrocyte and osteoblast are respectively planted in the areas A, B and C; the net-shaped bracket modified by the divided areas is rolled to form a cylindrical ligament graft with a physiological transition structure. The structure gradually transited from soft tissues to hard tissues effectively avoids the problem of stress concentration due to direct connection of the soft and hard tissues, solves the problem of weak biological fixation of the ligament graft-bone combination part in the existing single-phase tissue engineering ligament, and solves the problem that the seed cells cannot enter the deep part of the ligament in the traditional ligament weaving method; the ligament graft has the complete weaving structure, and solves the problem of weak connection between layers in the existing three-phase tissue engineering ligament.
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Description

technical field

[0001] The invention belongs to the field of medical tissue engineering, in particular to the field of three-phase silk ligament graft capable of reconstructing the normal anatomical structure of the ligament-bone joint. Background technique

[0002] Anterior cruciate ligament (ACL, anterior cruciate ligament) injury is a common disease of the knee joint. ACL injuries often lead to knee instability and degenerative osteoarthritis. Due to its limited ability to regenerate, it cannot repair itself after injury, so reconstruction must be through ligament grafting. There are two main sources of ligament grafts: ①autologous or allogeneic ligament transplantation; ②tissue engineered ligament.

[0003] At present, autologous ligament transplantation is mostly used clinically, but the source of autologous ligament transplantation is insufficient, and autologous ligament transplantation will cause many complications after taking materials. At present, 1 / 3 of the pa...

Examples

Embodiment Construction

[0051] The present invention will be further described below in conjunction with accompanying drawing.

[0052] An ideal tissue engineered ligament should have the following characteristics: ①The scaffold material has good biocompatibility; ②The tissue engineered ligament has sufficient mechanical strength and fatigue resistance; ③The maintenance time of the ligament strength must be long enough to benefit the ligament. Reconstruction; ④The ligament-bone junction needs to regenerate a typical four-layer structure of the ligament-bone junction (collagen fibers, fibrocartilage, calcified fibrocartilage, and bone), which is conducive to increasing the fixation strength of the ligament-bone junction and avoiding stress concentration; ⑤The seed cells can grow and proliferate on the scaffold material; ⑥The seed cells should be evenly distributed in the inner and outer layers of the ligament.

[0053] In order to meet the above requirements, biological reconstruction of tissue engine...