Duck viral hepatitis resisting astraglus polysaccharide phosphorylated molecular modification method

A technology of duck viral hepatitis and astragalus polysaccharides, which is applied in the direction of antiviral agents, organic active ingredients, and medical preparations containing active ingredients, can solve problems such as loss, and achieve the effect of convenient use and good water solubility of the preparation

CN105560352AActive Publication Date: 2016-05-11NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-05-11

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Abstract

The invention relates to a duck viral hepatitis (DVH) resisting astraglus polysaccharide phosphorylated molecular modification method, and belongs to the technical field of traditional Chinese veterinary medicine preparation. Different alcohol precipitation parts of astragalus polysaccharides are extracted, effective DVH-resisting astragalus polysaccharide parts are screened out through repeated clinical experiments, and then the optimal conditions for sodium tripolyphosphate-sodium trimetaphosphate method phosphorylated molecular modification of the astragalus polysaccharide effective parts are determined through an orthogonal method as the reaction temperature being 70 DEG C, the reaction time being 4 h and the pH being 8.5. The product obtained through infrared spectroscopic analysis is astragalus polysaccharide phosphate ester, the sugar content of astragalus polysaccharide phosphate ester reaches 36.87%, the phosphate radical content reaches 12.03%, and the yield of the product reaches 130.32%. The DHV-1 infected duct embryo liver cell resisting function and DVH curative effect of phosphate ester of the effective parts of astragalus polysaccharides are better than those of the effective parts of astragalus polysaccharides. The obtained phosphate ester of the effective parts of astragalus polysaccharides has high sugar and phosphate radical content and a high product yield, and a good curative effect on DVH is achieved.
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Description

1. Technical field

[0001] The invention relates to a method for modifying phosphorylated molecules of astragalus polysaccharides against duck viral hepatitis, and belongs to the technical field of preparation of traditional Chinese veterinary medicines. 2. Background technology

[0002] Duck viral hepatitis is a disease caused by duck hepatitis virus (DHV) infection that spreads rapidly, has a high morbidity rate, and is highly fatal. The virus was first isolated in 1949 and is now distributed worldwide. DHV mainly has three serotypes: DHV-1 and its variants, DuckAstrovirus and DHV-3, among which DHV-1 is the most toxic and widely distributed, mainly infecting ducklings under 3 weeks old, with a fatality rate higher than 80% %, even up to 100%, is one of the main pathogens that seriously endanger the duck industry.

[0003] At present, there is no effective anti-DHV-1 drug clinically in the world, and the immune defense is mainly carried out by injecting attenuated vaccine...

Examples

Embodiment Construction

[0016] 1. Extraction of astragalus polysaccharide and screening of effective parts

[0017] Weigh 1000g of astragalus, add 10 times the amount of water to soak for 3 hours, heat and boil for 1 hour, collect the decoction, and repeat 3 times; combine the collected decoction, boil and concentrate to 1000mL, centrifuge with a high-speed centrifuge, and take the supernatant. Slowly add ethanol with different volume fractions of 95% to the concentrated solution so that the ethanol content in the solution is 50%, 65%, 75% and 80% respectively, let it stand overnight, and collect the precipitates by centrifugation respectively. After dialysis with running water, vacuum concentration, 3000rpm / min, centrifugation for 20min, the supernatant was again added with different volume fractions of 95% ethanol for precipitation, repeated dialysis, concentration, and centrifugation, repeated 3 times, vacuum dried at 60°C, and crushed to obtain Astragalus membranaceus APS of different alcohol pre...