Manufacturing method of preparing insecticidal biopesticide by activating animal hormone

Abstract

The invention relates to a manufacturing method of preparing insecticidal biopesticide by activating an animal hormone. A comprehensive biological action of a toxin extracted and stored scientifically, reasonably and efficiently from live buthus martensii karsch, and a toxin of lumbricus, long-acting beauveria bassiana in bombyx batryticatus and the like is used for poisoning insect pests.The method is a technology of killing the pests by using the animal hormone and extracting active ingredients from the animal hormone.

Description

technical field

[0001] The invention relates to the field of agricultural preparations, in particular to a method for preparing insecticidal biopesticides by activating animal hormones. Background technique

[0002] Animal toxins are mostly protein compounds produced by poisonous animal venom glands and injected into other animals in the form of venom, such as snake venom, bee venom, scorpion venom, spider venom, centipede venom, ant venom, puffer fish venom, octopus venom, nereist venom, etc. And scallop toxin, saxitoxin, dolastatin, etc. produced by marine animals. There are also a variety of enzymes in the venom.

[0003] According to the biological effects of toxins, animal toxins can be divided into neurotoxins, cytotoxins, cardiotoxins, hemorrhagic toxins, hemolytic toxins, myotoxins or necrotic toxins.

[0004] The types of toxins and biological effects produced by different animals are different. For example, bee venom is mainly neurotoxin, hemolytic toxin and enzy...

Examples

Embodiment 1

[0020] An activated animal hormone system insecticidal biopesticide and a manufacturing method thereof, comprising the following steps:

[0021] 1) Preparation before production

[0022] ① Prepare raw materials: prepare clean East Asian scorpion live scorpion, earthworm, silkworm, ethanol, conventional pesticides;

[0023] ②Preparation equipment and tooling: vacuum refrigeration equipment, centrifuges, electronic pulse detoxification equipment, incubators, austenitic stainless steel containers, freezers, grinders;

[0024] 2) Preparation of protein toxin

[0025] ①Raise the incubator to 35°C, use an electronic pulse detoxification instrument in the incubator to stimulate and detoxify the clean East Asian pincer scorpion, and use austenitic stainless steel containers to collect the initial transparent venom, the middle milky white venom and the sticky tail Sexual venom, the first section of transparent venom and the tail section of viscous venom are reserved for later use, an...

Embodiment 2

[0034] A method for making activated animal hormones to make insecticidal biopesticides, the whole is consistent with Example 1, the difference is:

[0035] An activated animal hormone system insecticidal biopesticide and a manufacturing method thereof, comprising the following steps:

[0036] 2) Preparation of protein toxin

[0037] ①Raise the incubator to 32°C, use an electronic pulse detoxification instrument in the incubator to stimulate and detoxify the clean East Asian pincer scorpion live scorpion, and use austenitic stainless steel containers to collect the initial transparent venom, the middle milky white venom and the sticky tail Sexual venom, the first section of transparent venom and the tail section of viscous venom are reserved for later use, and the middle milky white venom is separated for other purposes;

[0038] ②Mix the initial transparent venom obtained in step ① and the tail viscous venom, and add it dropwise to an austenitic stainless steel container pla...

Embodiment 3

[0045] A method for making activated animal hormones to make insecticidal biopesticides, the whole is consistent with Example 1, the difference is:

[0046] 2) Preparation of protein toxin

[0047] ②Mix the initial transparent venom obtained in step ① and the tail viscous venom, and drop them into an austenitic stainless steel container placed in a freezer at -12°C within 15 minutes after extracting the scorpion body;

[0048]③ When the total weight of the total collected venom in the freezer reaches 1.1 parts by weight of the standard;

[0049] ④ Use a grinder to grind 15 parts by weight of the prepared earthworm and 12 parts by weight into powder, mix it evenly with the powder obtained in step ③, and store it in an environment of -12°C until use;

[0050] ⑥ Mix the prepared ethanol suspension with the conventional pesticide at a mass ratio of 1:800 to obtain the required activated animal hormone-based insecticidal biopesticide.

[0051] The insecticidal biopesticide based ...

technical field

[0001] The invention relates to the field of agricultural preparations, in particular to a method for preparing insecticidal biopesticides by activating animal hormones. Background technique

[0002] Animal toxins are mostly protein compounds produced by poisonous animal venom glands and injected into other animals in the form of venom, such as snake venom, bee venom, scorpion venom, spider venom, centipede venom, ant venom, puffer fish venom, octopus venom, nereist venom, etc. And scallop toxin, saxitoxin, dolastatin, etc. produced by marine animals. There are also a variety of enzymes in the venom.

[0003] According to the biological effects of toxins, animal toxins can be divided into neurotoxins, cytotoxins, cardiotoxins, hemorrhagic toxins, hemolytic toxins, myotoxins or necrotic toxins.

[0004] The types of toxins and biological effects produced by different animals are different. For example, bee venom is mainly neurotoxin, hemolytic toxin and enzy...

Examples

Embodiment 1

[0020] An activated animal hormone system insecticidal biopesticide and a manufacturing method thereof, comprising the following steps:

[0021] 1) Preparation before production

[0022] ① Prepare raw materials: prepare clean East Asian scorpion live scorpion, earthworm, silkworm, ethanol, conventional pesticides;

[0023] ②Preparation equipment and tooling: vacuum refrigeration equipment, centrifuges, electronic pulse detoxification equipment, incubators, austenitic stainless steel containers, freezers, grinders;

[0024] 2) Preparation of protein toxin

[0025] ①Raise the incubator to 35°C, use an electronic pulse detoxification instrument in the incubator to stimulate and detoxify the clean East Asian pincer scorpion, and use austenitic stainless steel containers to collect the initial transparent venom, the middle milky white venom and the sticky tail Sexual venom, the first section of transparent venom and the tail section of viscous venom are reserved for later use, an...

Embodiment 2

[0034] A method for making activated animal hormones to make insecticidal biopesticides, the whole is consistent with Example 1, the difference is:

[0035] An activated animal hormone system insecticidal biopesticide and a manufacturing method thereof, comprising the following steps:

[0036] 2) Preparation of protein toxin

[0037] ①Raise the incubator to 32°C, use an electronic pulse detoxification instrument in the incubator to stimulate and detoxify the clean East Asian pincer scorpion live scorpion, and use austenitic stainless steel containers to collect the initial transparent venom, the middle milky white venom and the sticky tail Sexual venom, the first section of transparent venom and the tail section of viscous venom are reserved for later use, and the middle milky white venom is separated for other purposes;

[0038] ②Mix the initial transparent venom obtained in step ① and the tail viscous venom, and add it dropwise to an austenitic stainless steel container pla...

Embodiment 3

[0045] A method for making activated animal hormones to make insecticidal biopesticides, the whole is consistent with Example 1, the difference is:

[0046] 2) Preparation of protein toxin

[0047] ②Mix the initial transparent venom obtained in step ① and the tail viscous venom, and drop them into an austenitic stainless steel container placed in a freezer at -12°C within 15 minutes after extracting the scorpion body;

[0048]③ When the total weight of the total collected venom in the freezer reaches 1.1 parts by weight of the standard;

[0049] ④ Use a grinder to grind 15 parts by weight of the prepared earthworm and 12 parts by weight into powder, mix it evenly with the powder obtained in step ③, and store it in an environment of -12°C until use;

[0050] ⑥ Mix the prepared ethanol suspension with the conventional pesticide at a mass ratio of 1:800 to obtain the required activated animal hormone-based insecticidal biopesticide.

[0051] The insecticidal biopesticide based ...