Polyphosphate fungicide and application thereof

Abstract

The invention relates to a polyphosphate fungicide and application thereof, which belongs to the technical field of biology. The polyphosphate fungicide is prepared by culturing transformed bacteria and acid-forming bacteria for 4 to 6 days under the condition of 26 to 30 DEG C, selecting transformed bacteria having an effect for degrading organic phosphorus to be prepared into first bacterial suspension, selecting acid-forming bacteria having an effect for degrading the organic phosphorus to be prepared into second bacterial suspension, and then mixing the first bacterial suspension and the second bacterial suspension. The transformed bacteria is prepared from at least one of cellulomonas and bacillus, and the acid-forming bacteria is prepared from acidophil. The phosphate fungicide has efficient and stable degradation capacity for the organic phosphorus, is high in fouling resistance and has high adaptability to a polluted environment. When the polyphosphate fungicide is used for degrading the organic phosphorus, the phosphorus pollution can be effectively treated, and environmental protection and energy conservation can be realized.

Description

technical field

[0001] The invention relates to the field of biotechnology, and in particular to a phosphorus-accumulating bacteria agent and its application. Background technique

[0002] Along with the rapid development of my country's chemical industry, the phosphorus chemical industry in my country has developed rapidly in the past two decades and has achieved encouraging results. However, with the development of Sinophosphorus chemical industry, the environmental pollution is becoming more and more serious.

[0003] Therefore, prevention and control of phosphorus chemical pollution, protection of the ecological environment, and rational use of non-renewable limited resources are an urgent task and an important issue for the healthy development of phosphorus chemical industry in my country.

[0004] At this stage, phosphorus removal methods include chemical precipitation, ion exchange, adsorption, membrane separation and biological methods. At present, there are some m...

Examples

Embodiment 1

[0040] The transformed bacteria and the acid-producing bacteria were activated at 30° C. for 24 hours, wherein the transformed bacteria contained Cellulomonas and the acid-producing bacteria contained acidophilus.

[0041] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0042] After the above medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and Acidophilus were inoculated in two different beef extract-peptone liquid medium respectively, and cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.3×10 7 cfu / mL; screen the acid-producing bacteria that can degrade organic phosphorus and prepare the turbi...

Embodiment 2

[0045] The transformed bacteria and the acid-producing bacteria were activated at 30°C for 24 hours, wherein the transformed bacteria contained Bacillus, and the acid-producing bacteria contained acidophilus.

[0046] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0047] After the above medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and Acidophilus were inoculated in two different beef extract-peptone liquid medium respectively, and cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.33×10 7 cfu / mL; screen the acid-producing bacteria that can degrade organic phosphorus and prepare the turbidity...

Embodiment 3

[0050] The transformed bacteria and acid-producing bacteria were activated at 30° C. for 24 hours, wherein the transformed bacteria contained Cellulomonas and Bacillus in a weight ratio of 0.8:1, and the acid-producing bacteria contained acidophilic bacteria.

[0051] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0052] After the above-mentioned medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and acidophilus were respectively inoculated in three different beef extract peptone liquid medium, and then cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.3×10 7 cfu / mL; screen the acid-producing bac...

technical field

[0001] The invention relates to the field of biotechnology, and in particular to a phosphorus-accumulating bacteria agent and its application. Background technique

[0002] Along with the rapid development of my country's chemical industry, the phosphorus chemical industry in my country has developed rapidly in the past two decades and has achieved encouraging results. However, with the development of Sinophosphorus chemical industry, the environmental pollution is becoming more and more serious.

[0003] Therefore, prevention and control of phosphorus chemical pollution, protection of the ecological environment, and rational use of non-renewable limited resources are an urgent task and an important issue for the healthy development of phosphorus chemical industry in my country.

[0004] At this stage, phosphorus removal methods include chemical precipitation, ion exchange, adsorption, membrane separation and biological methods. At present, there are some m...

Examples

Embodiment 1

[0040] The transformed bacteria and the acid-producing bacteria were activated at 30° C. for 24 hours, wherein the transformed bacteria contained Cellulomonas and the acid-producing bacteria contained acidophilus.

[0041] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0042] After the above medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and Acidophilus were inoculated in two different beef extract-peptone liquid medium respectively, and cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.3×10 7 cfu / mL; screen the acid-producing bacteria that can degrade organic phosphorus and prepare the turbi...

Embodiment 2

[0045] The transformed bacteria and the acid-producing bacteria were activated at 30°C for 24 hours, wherein the transformed bacteria contained Bacillus, and the acid-producing bacteria contained acidophilus.

[0046] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0047] After the above medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and Acidophilus were inoculated in two different beef extract-peptone liquid medium respectively, and cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.33×10 7 cfu / mL; screen the acid-producing bacteria that can degrade organic phosphorus and prepare the turbidity...

Embodiment 3

[0050] The transformed bacteria and acid-producing bacteria were activated at 30° C. for 24 hours, wherein the transformed bacteria contained Cellulomonas and Bacillus in a weight ratio of 0.8:1, and the acid-producing bacteria contained acidophilic bacteria.

[0051] Add 2 g of beef extract, 8 g of peptone, 4 g of sodium chloride and 15 g of agar into 1000 mL of water, adjust the pH of the mixed solution to 7.0, and obtain a liquid beef extract peptone medium.

[0052] After the above-mentioned medium was sterilized at high temperature and cooled, Cellulomonas, Bacillus and acidophilus were respectively inoculated in three different beef extract peptone liquid medium, and then cultured at 26°C for 6 days. Screening transformed bacteria that can degrade organophosphate to prepare turbidity OD 600 is the first bacterial suspension of 0.65, and the number of live bacteria of transformed bacteria in the first bacterial suspension is 0.3×10 7 cfu / mL; screen the acid-producing bac...

Images