Device and method for rapidly and quantitatively detecting bioactivity of dissolved organic matter (DOM) of water body

Abstract

The invention discloses a device and a method for rapidly and quantitatively detecting bioactivity of dissolved organic matter (DOM) of a water body on the basis of bio-membrane reactors. The device comprises at least four serially connected bio-membrane reactors, wherein the bio-membrane reactors comprise water inlets, water outlets and aeration holes; the aeration holes are connected with an aeration device; the bio-membrane reactors are a first-stage reactor, a second-stage reactor,...an Nth-stage reactor in sequence in a sample flowing direction; N is an integer greater than or equal to 4; the water outlet of the front one of each two adjacent reactors is communicated with the water inlet of the next reactor through a connection pipeline; and sampling holes are formed in the water outlets of the bio-membrane reactors. The method disclosed by the invention comprises the steps of biofilm culturing, sample detection and numerical fitting. By adopting the device and the method disclosed by the invention, the bioactivity of DOM of water bodies can be rapidly and quantitatively detected, DOM samples of different bioactivity degrees are acquired, and next steps of analysis can be implemented. The device and the method are short in time, high in efficiency and wide in application range.

Description

technical field

[0001] The invention belongs to the field of environmental analysis, and in particular relates to a device and method for rapidly and quantitatively detecting the biological activity of dissolved organic matter (DOM) in a water body based on a biofilm reactor. Background technique

[0002] The biological activity of dissolved organic matter (DOM) refers to the characteristics of DOM in water that can be metabolized and utilized by heterotrophic microorganisms. It mainly includes three parts: active DOM, semi-active DOM and refractory DOM. It is one of the main indicators for evaluating the biological stability of water bodies. , which is currently mainly used to predict and measure the carbon and nitrogen reduction load and carbon and nitrogen pool cycle in natural water bodies, as well as predict the potential generation trend of disinfection by-products in water bodies.

[0003] The existing DOM biological activity detection method is mainly the static inoc...

Examples

Embodiment 1

[0077] This example illustrates the specific implementation of the device of the present invention.

[0078] use as figure 1 The device for rapid quantitative detection of water body dissolved organic matter (DOM) biological activity based on the biofilm reactor as shown,

[0079] When using, include the following steps:

[0080] (1) Cultivate hanging film

[0081] Collect natural water and filter it with a carbon-free glass fiber membrane with a pore size of 3 μm to obtain inoculated water containing microorganisms and DOM; place the inoculated water in the sample bottle of the sampling device, and use the water pump to pump the inoculated water into the reaction device. In the biofilm reactor, the microorganisms in the inoculated water adhere to the surface of the porous glass microbeads in the biofilm reactor, and the DOM in the inoculated water acts as a carbon source to promote the enrichment of microorganisms and form a biofilm; the aeration pump passes the sterile air...

Embodiment 2

[0093] This embodiment 2 and embodiment 3 illustrate the specific application effect of the device and method of the present invention.

[0094] In this example, a water sample of a freshwater lake in Jiangsu was collected to measure its DOM biological activity. During the eutrophication period of the lake, the DOC concentration in the water body was as high as 40 mg / L.

[0095] In this embodiment, the sample bottle 1 has a volume of 3 liters, the middle aperture of the sterile rubber stopper 2 is 6 mm, and the outer diameter of the flexible tube 3 is 6 mm. The single-channel flow rate of the water delivery pump 4 is 0.05L / h, and the single-channel flow rate of the aeration pump 5 is 0.10L / h. The inner diameter of the bottom of the biofilm reactor 7 is 4.0cm, the height is 42cm, and the thickness of the outer wall is 2mm. The inner diameter of the bottom of the biofilm reactor 8 is 4.0cm, the height is 42cm, and the thickness of the outer wall is 2mm. , the height is 42cm, th...

Embodiment 3

[0102] In this example, a natural wetland water body in Jiangsu was collected to measure its DOM biological activity, and the DOC concentration of the wetland water body was about 5 mg / L. The difference between this embodiment and embodiment 3 is that the water sample to be tested is a natural wetland water body in Jiangsu. Other steps are identical with embodiment 3.

[0103] Filter a water sample from a wetland in Jiangsu with a carbon-free glass fiber filter membrane with a pore size of 0.7 μm, put the filtered water sample into the sampling device, start the power device, and collect No. 1, No. 2, No. 3, and No. 4 biofilms For the effluent water samples of the reactor, the DOC values ​​of the water samples to be tested and the effluent water samples of No. 1, No. 2, No. 3 and No. 4 biofilm reactors were measured by TOC. The results were 5.35, 3.62, 2.84, 2.28 and 1.90mg / L. Substitute the DOC value and t value into the following numerical models for fitting:

[0104] DOC...

technical field

[0001] The invention belongs to the field of environmental analysis, and in particular relates to a device and method for rapidly and quantitatively detecting the biological activity of dissolved organic matter (DOM) in a water body based on a biofilm reactor. Background technique

[0002] The biological activity of dissolved organic matter (DOM) refers to the characteristics of DOM in water that can be metabolized and utilized by heterotrophic microorganisms. It mainly includes three parts: active DOM, semi-active DOM and refractory DOM. It is one of the main indicators for evaluating the biological stability of water bodies. , which is currently mainly used to predict and measure the carbon and nitrogen reduction load and carbon and nitrogen pool cycle in natural water bodies, as well as predict the potential generation trend of disinfection by-products in water bodies.

[0003] The existing DOM biological activity detection method is mainly the static inoc...

Examples

Embodiment 1

[0077] This example illustrates the specific implementation of the device of the present invention.

[0078] use as figure 1 The device for rapid quantitative detection of water body dissolved organic matter (DOM) biological activity based on the biofilm reactor as shown,

[0079] When using, include the following steps:

[0080] (1) Cultivate hanging film

[0081] Collect natural water and filter it with a carbon-free glass fiber membrane with a pore size of 3 μm to obtain inoculated water containing microorganisms and DOM; place the inoculated water in the sample bottle of the sampling device, and use the water pump to pump the inoculated water into the reaction device. In the biofilm reactor, the microorganisms in the inoculated water adhere to the surface of the porous glass microbeads in the biofilm reactor, and the DOM in the inoculated water acts as a carbon source to promote the enrichment of microorganisms and form a biofilm; the aeration pump passes the sterile air...

Embodiment 2

[0093] This embodiment 2 and embodiment 3 illustrate the specific application effect of the device and method of the present invention.

[0094] In this example, a water sample of a freshwater lake in Jiangsu was collected to measure its DOM biological activity. During the eutrophication period of the lake, the DOC concentration in the water body was as high as 40 mg / L.

[0095] In this embodiment, the sample bottle 1 has a volume of 3 liters, the middle aperture of the sterile rubber stopper 2 is 6 mm, and the outer diameter of the flexible tube 3 is 6 mm. The single-channel flow rate of the water delivery pump 4 is 0.05L / h, and the single-channel flow rate of the aeration pump 5 is 0.10L / h. The inner diameter of the bottom of the biofilm reactor 7 is 4.0cm, the height is 42cm, and the thickness of the outer wall is 2mm. The inner diameter of the bottom of the biofilm reactor 8 is 4.0cm, the height is 42cm, and the thickness of the outer wall is 2mm. , the height is 42cm, th...

Embodiment 3

[0102] In this example, a natural wetland water body in Jiangsu was collected to measure its DOM biological activity, and the DOC concentration of the wetland water body was about 5 mg / L. The difference between this embodiment and embodiment 3 is that the water sample to be tested is a natural wetland water body in Jiangsu. Other steps are identical with embodiment 3.

[0103] Filter a water sample from a wetland in Jiangsu with a carbon-free glass fiber filter membrane with a pore size of 0.7 μm, put the filtered water sample into the sampling device, start the power device, and collect No. 1, No. 2, No. 3, and No. 4 biofilms For the effluent water samples of the reactor, the DOC values ​​of the water samples to be tested and the effluent water samples of No. 1, No. 2, No. 3 and No. 4 biofilm reactors were measured by TOC. The results were 5.35, 3.62, 2.84, 2.28 and 1.90mg / L. Substitute the DOC value and t value into the following numerical models for fitting:

[0104] DOC...

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