A modification method for improving immunological competence of milkvetch root-glossy privet fruit polysaccharides

A technology of immune activity and modification method, which is applied in the field of sulfation modification to improve the immune activity of Zhenqi polysaccharide, which can solve the problem of low biological activity of Zhenqi polysaccharide, achieve the effect of weak immune effect and improve immune activity

CN107915785AInactive Publication Date: 2018-04-17湖北回盛生物科技有限公司
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Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2018-04-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

A modification method for improving immunological competence of milkvetch root-glossy privet fruit polysaccharides is provided. The method includes a step of extraction, a step of milkvetch root-glossy privet fruit polysaccharide purification, and a step of performing sulfation modification through a chlorosulfonic acid-pyridine process to obtain sulfated milkvetch root-glossy privet fruit polysaccharides. By adopting product immunological competence as an index, L<9>(3<4>) orthogonal experiments are performed to optimize a reagent ratio, reaction temperature and reaction time which are therefactors, and determine optimum modification conditions in which the mass ratio of chlorosulfonic acid to pyridine is 1:8, the reaction temperature is 60 DEG C and the reaction time is 1 h, and the immunological competence of the sulfated milkvetch root-glossy privet fruit polysaccharides obtained under the optimum modification conditions is highest. The method can significantly improve immunological competence of the milkvetch root-glossy privet fruit polysaccharides.
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Description

technical field

[0001] The invention relates to the technical field of structural transformation of traditional Chinese medicine polysaccharides, in particular to a sulfation modification method for improving the immune activity of chastitylopolysaccharides. Background technique

[0002] Polysaccharides are widely found in animals, plants and microbial tissues. They are natural polymer compounds formed by the polymerization of monosaccharides. They are another important biological macromolecule in addition to proteins and nucleic acids in organisms. They have anti-virus, anti-tumor, anti- Oxidation, immune regulation and other biological activities. The biological activity of polysaccharides is closely related to its structure, especially when some chemical groups are introduced, the flexibility and spatial structure of sugar chains will change, thereby causing changes in the biological activities of polysaccharides or new activities of polysaccharides. Therefore, the appro...

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Embodiment Construction

[0017] The invention provides a modification method for improving the immune activity of chastostilbene polysaccharide. The steps include: extracting and purifying chastostilbene polysaccharide, suspending the chastostilbene polysaccharide in DMF, adding it to an esterification reagent, and shaking and reacting at 60-95°C 1 to 3 hours, after the reaction is completed, add ice water to terminate the reaction, cool down, and post-process to obtain sulfated chastostilbene polysaccharide; the preparation step of the esterification reagent includes: adding chlorosulfonic acid dropwise to pyridine, stirring and reacting to obtain the esterification reagent, The mass ratio of the chlorosulfonic acid to pyridine is 1:4˜1:8.

[0018] The present invention carried out sulfation modification on two mixed polysaccharides (Zhenqi polysaccharide) for the first time, and established a sulfation modification method, optimized the modification conditions based on the enhancement of immune activ...