An algae liquid online detection pool device

Abstract

The invention discloses an algae liquid online detection pool device, which belongs to the field of algae liquid detection technology. It includes a spectral information detection part and an image information detection part; the spectral information detection part includes an algae liquid spectral detection chamber and a spectral detection device; the spectral detection device includes a visible-near infrared light source generating device, a 470nm excitation fluorescent light source, a first spectrometer and a second spectrometer; the image information detection part includes an algae liquid image detection chamber, a light-emitting panel and a multi-spectral camera, the algae liquid image detection chamber is located on the right side of the spectral detection chamber shell, the multi-spectral camera is fixed on the multi-spectral camera fixing frame, so that the multi-spectral camera lens is aligned with the inner cavity of the image detection chamber shell through the right side wall of the image detection chamber shell, the inner cavity of the image detection chamber shell is the algae liquid image detection chamber, the bottom of the algae liquid spectral detection chamber is connected to the bottom of the algae liquid image detection chamber, and an algae liquid circulation outlet is provided at the top of the algae liquid image detection chamber. It has the characteristics of convenient and fast detection.

Description

Technical Field

[0001] The invention relates to the technical field of algae liquid detection. Background Art

[0002] In the process of microalgae cultivation, it is necessary to take samples to obtain the spectral information and image information of microalgae. Spectrometers and multispectral cameras are the two most common devices. After the light generated by the visible-near infrared light source passes through the algae liquid sample, part of the light energy is absorbed by the sample molecules, and the change in the incident light intensity is measured. After conversion, spectral information data such as absorbance can be obtained. The light from the 470nm excitation light source irradiates the algae liquid sample in the fluorescence pool, and part of the light energy is absorbed by the sample molecules. The sample molecules are excited and emit fluorescence, and the intensity of the emitted fluorescence is detected in a direction that forms a certain angle with the incident light path. Multispectral cameras can be used to obtain image information of microalgae at various growth stages. At present, the spectral information and image information detection of microalgae are carried out in the laboratory after on-site sampling, which has a certain lag, and the detection process is relatively cumbersome, and online real-time detection cannot be performed. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide an algae liquid online detection pool device, which has the characteristics of convenient and fast detection, and can simultaneously perform online real-time detection of spectral information and image information of the algae liquid.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] An algae liquid online detection pool device comprises a spectral information detection part and an image information detection part; the spectral information detection part comprises an algae liquid spectral detection chamber and a spectral detection device, the algae liquid spectral detection chamber comprises at least the following structures: comprising a spectral detection chamber shell, the spectral detection chamber shell adopts or is covered with a light-shielding material to shield ambient light from entering the algae liquid spectral detection chamber, the front side wall of the spectral detection chamber shell and the rear side wall of the spectral detection chamber shell are symmetrically provided with internal and external threaded interfaces for visible-near infrared light source transmission optical fibers and internal and external threaded interfaces for visible-near infrared light receiving optical fibers, respectively, and the top wall of the spectral detection chamber shell is provided with a Y-shaped fluorescence detection optical fiber insertion hole and an algae liquid inlet; the spectral detection device comprises a visible-near infrared light source generating device, a 470nm excitation fluorescence light source, a first spectrometer and a second spectrometer, the visible-near infrared light source transmission optical fiber connected to the internal and external threaded interfaces of the visible-near infrared light source transmission optical fiber is connected to the visible-near infrared light source generating device, and is used to transmit the light emitted by the visible-near infrared light source generating device to the algae liquid spectral detection chamber, and the visible-near infrared light receiving optical fiber is connected to the internal and external threaded interfaces of the visible-near infrared light receiving optical fiber. The receiving optical fiber is connected to the first spectrometer, one branch of the Y-shaped fluorescence detection optical fiber connected to the Y-shaped fluorescence detection optical fiber insertion hole is connected to a 470nm excitation fluorescence light source, and the other branch is connected to the second spectrometer. The inner cavity of the spectral detection chamber shell is an algae liquid spectral detection chamber; the image information detection part includes an algae liquid image detection chamber, a light-emitting panel and a multi-spectral camera. The algae liquid image detection chamber is located on the right side of the spectral detection chamber shell. The algae liquid image detection chamber at least includes the following structures: including an image detection chamber shell, the left and right walls of the image detection chamber shell are both made of transparent materials, a rectangular groove is provided between the right side wall of the spectral detection chamber shell and the left side wall of the image detection chamber shell, forming a light-emitting panel slot, and the light-emitting panel is embedded in the light-emitting panel slot. A multi-spectral camera fixing frame is provided on the right side of the image detection chamber shell, and the multi-spectral camera is fixed on the multi-spectral camera fixing frame so that the multi-spectral camera lens is aligned with the inner cavity of the image detection chamber shell through the right side wall of the image detection chamber shell, and the inner cavity of the image detection chamber shell is an algae liquid image detection chamber. The bottom of the algae liquid spectral detection chamber is connected to the bottom of the algae liquid image detection chamber, and an algae liquid circulation outlet is provided at the top of the algae liquid image detection chamber.

[0006] The present invention is further improved in that:

[0007] It also includes an algae liquid precipitation cleaning device: an air inlet is provided on the top wall of the spectrum detection chamber shell, and an air blowing pipe is provided in the algae liquid spectrum detection chamber. The upper end of the air blowing pipe is connected to the air inlet, and the lower end of the air blowing pipe extends to the bottom of the algae liquid spectrum detection chamber. A sewage outlet is provided near the bottom of the spectrum detection chamber shell.

[0008] A filter funnel is provided in the algae liquid inlet to filter the algae liquid entering the algae liquid spectrum detection chamber.

[0009] The shell of the spectrum detection chamber and the shell of the image detection chamber are both square shells.

[0010] The beneficial effects of adopting the above technical solution are:

[0011] The invention has a scientific and reasonable structure, a light structure, a small volume, a flexible configuration and is easy to move. It is specially used for algae liquid detection of near-infrared-visible light absorption spectrum, fluorescence spectrum and image information in the microalgae cultivation process, and can simultaneously measure the absorption spectrum of the algae liquid, the in-situ fluorescence spectrum and image information online in real time. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the present invention;

[0013] Figure 2 yes Figure 1 Center front section view;

[0014] Figure 3 yes Figure 1 A top sectional view of

[0015] Figure 4 yes Figure 1 Schematic diagram of the internal structure of the algae liquid inlet.

[0016] In the attached drawings: 1. Spectral detection chamber shell; 2. Internal and external threaded interfaces for visible-near-infrared light source transmission optical fiber; 3. Internal and external threaded interfaces for visible-near-infrared light receiving optical fiber; 4. Y-shaped fluorescence detection optical fiber insertion hole; 5. Algae solution inlet; 6. Visible-near-infrared light source transmission optical fiber; 7. Visible-near-infrared light receiving optical fiber; 8. Y-shaped fluorescence detection optical fiber; 8-1. One branch of the Y-shaped fluorescence detection optical fiber; 8-2. Another branch 8-2 ​​of the Y-shaped fluorescence detection optical fiber; 9. Image detection chamber shell; 10. Rectangular groove; 11. Multi-spectral camera fixing bracket; 13. Air inlet; 14. Air blowing tube; 15. Algae solution inlet; 16. Filter funnel; 17. Multi-spectral camera. DETAILED DESCRIPTION

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] Depend on Figure 1-4It can be seen from the embodiment shown that this embodiment includes a spectral information detection part and an image information detection part; the spectral information detection part includes an algae liquid spectral detection chamber and a spectral detection device, and the algae liquid spectral detection chamber includes at least the following structures: including a spectral detection chamber shell 1, the spectral detection chamber shell is made of or covered with a light-shielding material to shield ambient light from entering the algae liquid spectral detection chamber, and the front side wall of the spectral detection chamber shell 1 and the rear side wall of the spectral detection chamber shell are symmetrically provided with an internal and external threaded interface 2 for a visible-near-infrared light source transmission optical fiber and an internal and external threaded interface 3 for a visible-near-infrared light receiving optical fiber, respectively, and the top wall of the spectral detection chamber shell 1 is provided with a Y-shaped fluorescence detection optical fiber insertion hole 4 and an algae liquid spectral detection optical fiber insertion hole 5. Liquid inlet 5; the spectrum detection device includes a visible-near infrared light source generating device (using a halogen lamp as a light source, model: JY-L2000), a 470nm excitation fluorescence light source (model: SMA905), a first spectrometer (model: USB6500-pro) and a second spectrometer (model: USB4000), a visible-near infrared light source transmission optical fiber 6 connected to the visible-near infrared light source transmission optical fiber with an internal and external threaded interface 2 is connected to the visible-near infrared light source generating device, and is used to transmit the light emitted by the visible-near infrared light source generating device to the algae liquid spectrum detection chamber, and a visible-near infrared light receiving optical fiber connected to the internal and external threaded interface 3 The visible-near infrared light receiving optical fiber 7 is connected to the first spectrometer, one branch 8-1 of the Y-shaped fluorescence detection optical fiber 8 connected to the Y-shaped fluorescence detection optical fiber insertion hole 4 is connected to the 470nm excitation fluorescence light source, and the other branch 8-2 ​​is connected to the second spectrometer. The inner cavity of the spectrum detection chamber shell 1 is the algae liquid spectrum detection chamber; the image information detection part includes an algae liquid image detection chamber, a light emitting panel (model: HF-FX100) and a multi-spectral camera 17 (model: AD-130GE). The algae liquid image detection chamber is located on the right side of the spectrum detection chamber shell 1. The algae liquid image detection chamber includes at least the following structures: including an image detection chamber shell 9, an image detection chamber shell 9 The left and right walls are both made of transparent material. A rectangular groove 10 is provided between the right side wall of the spectral detection chamber shell 1 and the left side wall of the image detection chamber shell 9 to form a light-emitting panel slot. The light-emitting panel is embedded in the light-emitting panel slot. A multi-spectral camera fixing bracket 11 is provided on the right side of the image detection chamber shell 9. The multi-spectral camera 17 is fixed on the multi-spectral camera fixing bracket 11 so that the multi-spectral camera lens is aligned with the inner cavity of the image detection chamber shell 9 through the right side wall of the image detection chamber shell 9. The inner cavity of the image detection chamber shell 9 is an algae liquid image detection chamber. The bottom of the algae liquid spectral detection chamber is connected to the bottom of the algae liquid image detection chamber, and an algae liquid circulation outlet 12 is provided at the top of the algae liquid image detection chamber.

[0019] It also includes an algae liquid precipitation cleaning device: an air inlet 13 is provided on the top wall of the spectrum detection chamber shell 1, and an air blowing pipe 14 is provided in the algae liquid spectrum detection chamber. The upper end of the air blowing pipe 14 is connected to the air inlet 13, and the lower end of the air blowing pipe 14 extends to the bottom of the algae liquid spectrum detection chamber. A sewage outlet 15 is provided near the bottom of the spectrum detection chamber shell 1.

[0020] A filter funnel 16 is provided in the algae liquid inlet 5 to filter the algae liquid entering the algae liquid spectrum detection chamber.

[0021] The spectrum detection chamber housing 1 and the image detection chamber housing 9 are both square housings.

[0022] The light emitting panel, the first spectrometer and the second spectrometer are not shown in the figure, but it does not affect the understanding of the present invention.

[0023] Working principle:

[0024] Before use, the device is connected to the microalgae cultivation pond through a pipeline to form a circulation system. The algae liquid to be tested is pumped into the algae liquid inlet 5 through the pipeline by the liquid inlet pump. The algae liquid sample to be tested enters the algae liquid spectrum detection chamber from the algae liquid inlet 5, and then flows into the algae liquid image detection chamber from the bottom of the algae liquid spectrum detection chamber, and returns to the microalgae cultivation pond through the pipeline from the algae liquid circulation outlet 12 at the top of the algae liquid image detection chamber.

[0025] During the online monitoring process, three working modes of visible-near infrared absorption spectrum, fluorescence spectrum and image information detection can be realized. In the visible-near infrared absorption spectrum detection mode, the light emitted from the halogen lamp light source is introduced into the algae liquid spectrum detection chamber by the visible-near infrared light source transmission optical fiber 6, and after being absorbed by the algae liquid sample in the algae liquid spectrum detection chamber, it is introduced into the first spectrometer by the visible-near infrared light receiving optical fiber 7, and the first spectrometer detects the spectral information data such as absorbance; the 470nm excitation fluorescence light source is introduced into the algae liquid spectrum detection chamber through a branch 8-1 of the Y-shaped fluorescence detection optical fiber 8 to excite the fluorescent active substance in the algae liquid sample in the algae liquid spectrum detection chamber. The fluorescence emitted by the fluorescent active substance after being excited forms a certain angle with the incident fluorescence light path and is introduced into the second spectrometer by another branch 8-2 ​​of the Y-shaped fluorescence detection optical fiber 8 to measure its fluorescence spectrum; the light emitted by the white light emitting panel is irradiated into the algae liquid image detection chamber through the left side wall (transparent material) of the image detection chamber shell 9, and at the same time, the lens of the multi-spectral camera 17 collects image information of the algae liquid sample in the algae liquid image detection chamber through the right side wall (transparent material) of the image detection chamber shell 9.

[0026] As the usage time increases, algae precipitates will be produced at the bottom of the algae liquid spectrum detection chamber and the algae liquid image detection chamber. Gas under a certain pressure is pumped into the bottom of the algae liquid spectrum detection chamber and the algae liquid image detection chamber from the air inlet through the air blowing pipe 14. Since algae are not easy to stick, the algae precipitates will be blown up. The valve on the sewage outlet 15 is opened and the algae precipitates are discharged from the sewage outlet 15.

Claims

1. An algae liquid online detection pool device, characterized in that: The device comprises a spectral information detection part and an image information detection part; the spectral information detection part comprises an algae liquid spectral detection chamber and a spectral detection device, the algae liquid spectral detection chamber comprises at least the following structures: comprising a spectral detection chamber shell (1), the spectral detection chamber shell adopts or is covered with a light-shielding material to shield ambient light from entering the algae liquid spectral detection chamber, the front side wall and the rear side wall of the spectral detection chamber shell (1) are symmetrically provided with an internal and external threaded interface (2) for a visible-near infrared light source transmission optical fiber and an internal and external threaded interface (3) for a visible-near infrared light receiving optical fiber, and the top wall of the spectral detection chamber shell (1) is provided with a Y-shaped fluorescence detection optical fiber plug The spectral detection device comprises a visible-near-infrared light source generating device, a 470nm excitation fluorescence light source, a first spectrometer and a second spectrometer. The visible-near-infrared light source transmission optical fiber (6) connected to the visible-near-infrared light source generating device by the internal and external threaded interface (2) is connected to the visible-near-infrared light source generating device, and is used to transmit the light emitted by the visible-near-infrared light source generating device to the algae liquid spectral detection chamber. The visible-near-infrared light receiving optical fiber (7) connected to the internal and external threaded interface (3) is connected to the first spectrometer. The Y-shaped fluorescence detection optical fiber insertion hole (4) is connected to the Y-shaped fluorescence detection optical fiber. One branch (8-1) of the shaped fluorescence detection optical fiber (8) is connected to a 470nm excitation fluorescence light source, and the other branch (8-2) is connected to the second spectrometer; the inner cavity of the spectrum detection chamber shell (1) is an algae liquid spectrum detection chamber; the image information detection part comprises an algae liquid image detection chamber, a light emitting panel and a multi-spectral camera (17); the algae liquid image detection chamber is located on the right side of the spectrum detection chamber shell (1); the algae liquid image detection chamber comprises at least the following structures: comprising an image detection chamber shell (9); the left side wall and the right side wall of the image detection chamber shell (9) are both made of transparent material; the right side wall of the spectrum detection chamber shell (1) is connected to the image detection chamber shell ( 9), a rectangular groove (10) is provided between the left side walls thereof, forming a light-emitting panel slot, the light-emitting panel is embedded in the light-emitting panel slot, a multi-spectral camera fixing frame (11) is provided on the right side of the image detection chamber housing (9), the multi-spectral camera (17) is fixed on the multi-spectral camera fixing frame (11), so that the multi-spectral camera lens is aligned with the inner cavity of the image detection chamber housing (9) through the right side wall of the image detection chamber housing (9), the inner cavity of the image detection chamber housing (9) is the algae liquid image detection chamber, the bottom of the algae liquid spectrum detection chamber is connected to the bottom of the algae liquid image detection chamber, and an algae liquid circulation outlet (12) is provided at the top of the algae liquid image detection chamber; It also includes an algae liquid sediment cleaning device: an air inlet (13) is provided on the top wall of the spectrum detection chamber housing (1), and an air blowing pipe (14) is provided in the algae liquid spectrum detection chamber; The upper end of the air blowing pipe (14) is connected to the air inlet (13), and the lower end of the air blowing pipe (14) extends to the bottom of the algae liquid spectrum detection chamber, and a sewage outlet (15) is provided near the bottom of the spectrum detection chamber shell (1); Before use, the algae liquid online detection pool device is connected to the microalgae cultivation pool through a pipeline to form a circulation system, and the algae liquid to be tested is pumped into the algae liquid inlet (5) through the pipeline by the liquid inlet pump. The algae liquid sample to be tested enters the algae liquid spectrum detection chamber from the algae liquid inlet (5), and then flows into the algae liquid image detection chamber from the bottom of the algae liquid spectrum detection chamber, and flows back to the microalgae cultivation pool from the algae liquid circulation outlet (12) at the top of the algae liquid image detection chamber through the pipeline; As the use time increases, algae sediment will be generated at the bottom of the algae liquid spectrum detection chamber and the algae liquid image detection chamber. A certain pressure of gas is injected into the bottom of the algae liquid spectrum detection chamber and the algae liquid image detection chamber from the air inlet through the air blowing pipe (14). Since algae are not easy to adhere, the algae sediment will be blown up. The valve on the sewage outlet (15) is opened, and the algae sediment is discharged from the sewage outlet (15).

2. The algae liquid online detection pool device according to claim 1, characterized in that: A filter funnel (16) is provided in the algae liquid inlet (5) to filter the algae liquid entering the algae liquid spectrum detection chamber.

3. The algae liquid online detection pool device according to claim 2, characterized in that: The spectrum detection chamber housing (1) and the image detection chamber housing (9) are both square housings.

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