Cell Slide Machine

Abstract

The present invention relates to a cell slice making machine, a frame, a liquid transfer device and a suction filtration device. The cell slice making machine can automatically collect cells without too much manual operation, and the collection rate and stable maintenance of rare cells such as CTC are significantly improved. The cell slice making machine can automatically identify the label information on the sample and the filter, and can avoid the mistakes that may be caused by manual operation. The cell slice making machine is used to collect rare cells, and automatic operations can be realized from liquid addition, filtration collection, cell fixation, dehydration after fixation, etc., and the sample processing flow can be completed at one time, reducing the manual processing time, saving a lot of experimental time, and significantly improving the collection efficiency.

Description

Technical Field

[0001] The present invention relates to the field of liquid biopsy equipment, and in particular to a cell slice making machine. Background Art

[0002] Liquid biopsy is a hot topic in current medical testing. It is a fast and simple blood test method for cancer, one of the important ways of precision medicine, and a non-invasive and repeatable means of extracting tumor samples. The application of liquid biopsy technology can achieve real-time monitoring of the entire process of cancer prevention, diagnosis, and prognosis. Liquid biopsy has broad application prospects in early in vitro diagnosis, rapid evaluation of chemotherapy drugs, individualized treatment including clinical drug screening and drug resistance detection, monitoring of tumor recurrence, and development of new tumor drugs.

[0003] Non-blood-derived rare cells refer to other non-blood-derived cells in the blood after various blood-derived cells (red blood cells, white blood cells and platelets, etc.) are removed. They mainly include: circulating tumor cells (CTCs), circulating endothelial cells, circulating epithelial cells, tumor stem cells, etc. Non-blood-derived rare cells represented by circulating tumor cells are the main detection objects and research contents of liquid biopsy. They undergo apoptosis or are phagocytosed after entering the peripheral blood, but a few can escape and develop into metastatic lesions, increasing the risk of death in patients with malignant tumors. Malignant tumors will spread to other organs of the body through the blood, and tumor metastasis is the main cause of death in tumor patients. Tumor cells invade the surrounding tissues of primary tumor cells, enter the blood and lymphatic system, form circulating tumor cells, and are transported to distal tissues, then infiltrate, adapt to the new microenvironment, and finally "seed", "proliferate", "colonize", and form metastatic lesions.

[0004] Since rare cells are rare in peripheral blood, their frequency in patients with metastatic tumors is less than one millionth of normal white blood cells (white blood cells: (4-11) × 10 6 CTCs are difficult to capture, but their enrichment is a key step in liquid biopsy. At present, the methods for enriching CTCs mainly include immunomagnetic bead adsorption, gradient centrifugation, membrane filtration, etc. As a simple and efficient method, membrane filtration has been applied by many researchers to enrich CTCs. However, the current membrane filtration capture of CTCs is mainly done manually. The manual operation has a single throughput, low efficiency, and a small number of samples processed per day, which cannot meet the needs of various medical institutions, and brings huge problems to the later application industrialization; at the same time, the manual operation process is cumbersome, there are manual deviations, and the detection quality and stability are greatly reduced, which is not in line with the current growing detection needs. Summary of the invention

[0005] Based on this, it is necessary to provide a cell slice making machine that can improve the collection efficiency.

[0006] A cell slice making machine, comprising:

[0007] A rack, comprising a base and a support frame mounted on the base, wherein the base is provided with a plurality of placement positions, and the plurality of placement positions are respectively used to place a tip rack, a reagent tank, a filter, a reagent strip tank, a sample rack and a waste tank; and

[0008] A pipetting device is installed on the support frame and is located above the placement position. The pipetting device includes a position adjustment mechanism, a liquid suction mechanism, a removal mechanism and a scanning mechanism. The liquid suction mechanism, the removal mechanism and the scanning mechanism are connected to the position adjustment mechanism and can be driven by the position adjustment mechanism to move between different placement positions. The liquid suction mechanism is used to load a tip from the tip rack to suck the corresponding liquid into the filter or release it from the waste tank. The removal mechanism is used to remove the tip installed on the liquid suction mechanism and the rubber plug on the sample tube to the waste tank. The scanning mechanism is used to scan the labels of the sample tube and the filter on the sample rack.

[0009] In one embodiment, the position adjustment mechanism includes a lateral motion module and a vertical motion module; the lateral motion module includes a lateral drive device, a lateral transmission assembly and a lateral motion seat; the vertical motion module includes a vertical drive device, a vertical transmission assembly, a vertical fixed seat and a vertical motion seat; the lateral motion seat is movably arranged on the support frame and can move in the lateral direction, the vertical fixed seat is fixed on the lateral motion seat, the vertical motion seat is movably arranged on the vertical fixed seat and can move in the vertical direction, and the liquid suction mechanism and the removal mechanism are arranged on the vertical motion seat;

[0010] The transverse driving device is fixed on the support frame, and the transverse driving device is connected to the transverse motion seat through the transverse transmission assembly to drive the transverse motion seat to move and drive the vertical fixed seat to move in the transverse direction;

[0011] The vertical driving device is fixed on the lateral motion seat or the vertical fixed seat, and the vertical driving device is connected to the vertical motion seat through the vertical transmission assembly to drive the liquid suction mechanism and the removal mechanism to move vertically by driving the vertical motion seat to move.

[0012] In one embodiment, the transverse driving device is a motor, the transverse transmission assembly includes a transmission wheel / transmission belt linkage and a screw rod / screw nut linkage, the transverse motion seat is fixedly connected to the screw rod nut of the screw rod / screw nut linkage, and the transverse driving device drives the screw rod of the screw rod / screw nut linkage to rotate through the transmission wheel / transmission belt linkage, thereby driving the transverse motion seat to move along the screw rod extending laterally;

[0013] The vertical driving device is a motor, and the vertical transmission assembly includes a transmission wheel / transmission belt linkage and a screw rod / screw rod nut linkage. The vertical motion seat is fixedly connected to the screw rod nut of the screw rod / screw rod nut linkage. The vertical driving device drives the screw rod of the screw rod / screw rod nut linkage to rotate through the transmission wheel / transmission belt linkage, thereby driving the vertical motion seat to move along the screw rod extending vertically.

[0014] In one of the embodiments, the support frame is provided with a transverse slide rail, and the transverse motion seat is slidably connected to the transverse slide rail; the vertical fixed seat is provided with a vertical slide rail, and the vertical motion seat is slidably connected to the vertical slide rail.

[0015] In one embodiment, the liquid suction mechanism includes a liquid suction drive device, a liquid suction push block, a liquid suction device and a liquid suction head;

[0016] The liquid suction driving device is arranged on the vertical motion seat, the liquid suction pushing block is located above the liquid suction device and is connected to the piston rod of the liquid suction device, the liquid suction driving device is connected to the liquid suction pushing block to drive the piston rod to move by driving the liquid suction pushing block to move, the liquid suction head is located below the liquid suction device and is internally connected to the liquid suction device, and the liquid suction head is used to install the suction head.

[0017] In one embodiment, the liquid suction drive device is a motor, and the liquid suction drive device is connected to the liquid suction push block via a screw nut of a screw / screw nut linkage; and / or

[0018] There are multiple pipettes and pipette heads, the piston rods of the multiple pipettes are connected to the pipette push block to be driven by the pipette push block to move synchronously, and the multiple pipettes correspond to the multiple pipette heads one by one; and / or

[0019] A plug-pulling needle for inserting through the rubber plug of the sample tube is provided below the liquid-pipette head, and the plug-pulling needle is connected with the liquid-pipette device through the liquid-pipette head.

[0020] In one embodiment, the stripping mechanism includes a stripping drive device, a stripping push block, a stripping pressure plate and a stripping pressure plate push shaft;

[0021] The withdrawal drive device is arranged on the vertical motion seat, the withdrawal drive device is connected to the withdrawal push block, the withdrawal push block is connected to the withdrawal pressure plate through the withdrawal pressure plate push shaft, the withdrawal pressure plate is sleeved on the liquid suction head, the withdrawal pressure plate can be driven by the withdrawal drive device to move vertically along the liquid suction head, the bottom of the withdrawal pressure plate is provided with an assembly groove surrounding the liquid suction head, and there is a gap between the side wall of the assembly groove and the liquid suction head for the suction head to be inserted.

[0022] In one embodiment, the removal drive device is an electric push rod; and / or

[0023] There are multiple removal drive devices; and / or

[0024] A vertically extending guide rod is provided on the vertical motion seat, the guide rod passes through the liquid suction push block and the removal push block, and the liquid suction push block and the removal push block can move along the guide rod; and / or

[0025] The removal driving device and the liquid suction driving device are respectively located on two sides of the vertical motion seat.

[0026] In one embodiment, the scanning mechanism includes a scanning fixed seat, a scanning driving device, a scanning transmission component, a scanning positioning bracket and a scanner;

[0027] The scanning fixed seat is fixed on the vertical fixed seat, the scanning drive device, the scanning transmission assembly and the scanning positioning bracket are fixed on the scanning fixed seat, the scanning positioning bracket has a plurality of scanning windows and the plurality of scanning windows are arranged in sequence along the longitudinal direction, the scanning drive device is connected to the scanner through the scanning transmission assembly to drive the scanner to move along the longitudinal direction to scan the label information corresponding to different scanning windows.

[0028] In one embodiment, the cell preparation machine further comprises a suction filtration device;

[0029] The suction filtration device comprises a suction filtration joint and a negative pressure pump, and the negative pressure pump is connected to the filter through the suction filtration joint to suck the liquid in the filter and discharge it.

[0030] In one embodiment, the suction filtration connector is a multi-way connector, which has a connector connected to the filter, a connector connected to the negative pressure pump, and a connector connected to an external washing liquid container.

[0031] In one embodiment, there are multiple filters, the suction filter connector is a Luer three-way connector, there are multiple negative pressure pumps, and the multiple filters, multiple connectors of the suction filter connector and multiple negative pressure pumps are connected respectively.

[0032] In one embodiment, there are multiple tip racks; and / or there are multiple reagent tanks; and / or the waste tank includes a waste tank and a waste material tank.

[0033] In one embodiment, the cell preparation machine also includes a sample fixing mechanism; the sample fixing mechanism is arranged below the base, the base is hollowed out corresponding to the placement position of the sample rack, the sample tube can pass through the base and extend into the sample fixing mechanism, and the sample fixing mechanism is used to fix the sample tube on the sample rack.

[0034] In one embodiment, the sample fixing mechanism includes an electric cylinder driving device, a fixing spring and a fixing block; the electric cylinder driving device is connected to the fixing block via the fixing spring, the sample tube can be inserted between the fixing block and the electric cylinder driving device, and the electric cylinder driving device can clamp the sample tube and limit it on the sample rack via the fixing block.

[0035] The above-mentioned cell slide machine can automatically collect cells without too much manual operation, and has significantly improved the collection rate and stable maintenance of rare cells such as CTC. The cell slide machine can automatically identify the label information on the sample and the filter, which can avoid the mistakes that may be caused by manual operation. The cell slide machine can be used to collect rare cells, and automatic operations can be achieved from adding liquid, filtering collection, cell fixation, dehydration after fixation, etc. The sample processing process can be completed in one go, reducing manual processing time, saving a lot of experimental time, and significantly improving collection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is an overall side view of a cell slide making machine according to an embodiment of the present invention;

[0037] Figure 2 for Figure 1 A schematic diagram of the distribution of the middle base and different placement positions thereon;

[0038] Figure 3 for Figure 2 a side view of the structure shown and the sample securing mechanism thereunder;

[0039] Figure 4 for Figure 1 A schematic diagram of the structure of the liquid transfer device;

[0040] Figure 5 for Figure 4 A schematic structural diagram of the pipetting device from another perspective;

[0041] Figure 6 for Figure 4 A schematic diagram of the structure of the air pressure plug puller structure;

[0042] Figure 7 for Figure 6 A schematic structural diagram of the air pressure plug structure from another perspective;

[0043] Figure 8 for Figure 6 A schematic diagram of the plug-pulling process of the pneumatic plug-pulling structure shown;

[0044] Fig. 9 for Figure 1 Schematic diagram of the connection structure between the suction filtration device and the suction filter. DETAILED DESCRIPTION

[0045] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0046] It should be noted that when an element is referred to as being "fixed to" or "mounted on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element at the same time.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0048] like Figure 1 As shown, an embodiment of the present invention provides a cell preparation machine 10 , which includes a frame 100 and a pipetting device 200 .

[0049] Please combine Figure 1 , Figure 2 and Figure 3 The rack 100 includes a base 110 and a support frame 120 mounted on the base 110. The base 110 is provided with a plurality of placement positions, which are respectively used to place a tip rack 111, a reagent tank 112, a filter 113, a reagent strip tank 114, a sample rack 115 and a waste tank 116. The tip rack 111 is used to place a tip 20. The reagent tank 112 is used to hold various reagents. The reagent strip tank 114 is used to place a reagent strip 30. The sample rack 115 is used to place a sample tube 40. The waste tank 116 is used to store waste liquid and waste materials such as tips and rubber plugs.

[0050] In the specific example shown in the figure, there are multiple tip racks 111, and the multiple tip racks 111 are located at different positions of the base 110, so that the pipetting device 200 can easily load and unload the tips 20 at multiple different positions; there are also multiple reagent tanks 112 for holding different reagents; the waste tank 116 preferably includes a waste liquid tank 1161 for storing waste liquid and a waste tank 1162 for storing solid waste such as tips and plugs.

[0051] More specifically, in the illustrated example, from one end to the other end of the base 110, a tip rack 111, a plurality of reagent slots 112, a filter 113, another tip rack 111, a reagent strip slot 114, a sample rack 115, a waste slot 1161 and a waste slot 1162 are sequentially distributed along the lateral direction.

[0052] Further, if Figure 3 As shown, in the specific example shown in the figure, the cell slide making machine 10 further includes a sample fixing mechanism 400. The sample fixing mechanism 400 is disposed below the base 110, and the base 110 is hollowed out corresponding to the placement position of the sample rack 115, and the sample tube 40 can pass through the base 110 and extend into the sample fixing mechanism 400. The sample fixing mechanism 400 is used to fix the sample tube 40 on the sample rack 115.

[0053] Specifically, the sample fixing mechanism 400 includes an electric cylinder driving device 410, a fixing spring 420 and a fixing block 430. The electric cylinder driving device 410 is connected to the fixing block 430 through the fixing spring 420, and the sample tube 40 can extend between the fixing block 430 and the electric cylinder driving device 410. The electric cylinder driving device 410 can clamp the sample tube 40 on the sample rack 115 through the fixing block 430.

[0054] The pipetting device 200 of this embodiment is installed on the support frame 120 and is located above the placement position of the base 110. The pipetting device 200 includes a position adjustment mechanism 210, a liquid suction mechanism 220, a removal mechanism 230 and a scanning mechanism 240. The liquid suction mechanism 220, the removal mechanism 230 and the scanning mechanism 240 are connected to the position adjustment mechanism 210 so as to be driven by the position adjustment mechanism 210 to move between different placement positions on the base 110. Among them, the liquid suction mechanism 220 is used to load the suction tip 20 from the suction tip rack 111 to suck the corresponding liquid into the filter 113 or the waste tank 116 for discharge; the removal mechanism 230 is used to remove the suction tip 20 installed on the liquid suction mechanism 220 to the waste tank 116; the scanning mechanism 240 is used to scan the labels of the sample tube 40 and the filter 113 on the sample rack 115.

[0055] Please combine Figure 4 and Figure 5In a specific example, the position adjustment mechanism 210 includes a lateral motion module 211 and a vertical motion module 212. The lateral motion module 211 includes a lateral drive device 2111, a lateral transmission assembly 2112 and a lateral motion seat 2113. The vertical motion module 212 includes a vertical drive device 2121, a vertical transmission assembly 2122, a vertical fixed seat 2123 and a vertical motion seat 2124. The lateral motion seat 2113 is movably arranged on the support frame 120 and can move in the lateral direction. The vertical fixed seat 2123 is fixed on the lateral motion seat 2113. The vertical motion seat 2124 is movably arranged on the vertical fixed seat 2123 and can move vertically. The liquid suction mechanism 220 and the removal mechanism 230 are arranged on the vertical motion seat 2124.

[0056] The lateral drive device 2111 is fixed on the support frame 120, and the lateral drive device 2111 is connected to the lateral motion seat 2113 through the lateral transmission assembly 2112 to drive the vertical fixed seat 2123 to move in the lateral direction by driving the lateral motion seat 2113. The vertical drive device 2121 is fixed on the lateral motion seat 2113 or the vertical fixed seat 2123. The vertical drive device 2121 is connected to the vertical motion seat 2124 through the vertical transmission assembly 2122 to drive the liquid suction mechanism 220 and the removal mechanism 230 to move in the vertical direction by driving the vertical motion seat 2124.

[0057] In the specific example shown in the figure, the transverse drive device 2111 is a motor. The transverse transmission assembly 2112 includes a transmission wheel / transmission belt linkage and a screw rod / screw nut linkage (not shown in the figure). The transverse motion seat 2113 is fixedly connected to the screw nut of the screw rod / screw nut linkage. The transverse drive device 2111 drives the screw of the screw rod / screw nut linkage to rotate through the transmission wheel / transmission belt linkage, thereby driving the transverse motion seat 2113 fixedly connected to the screw rod nut to move along the transverse extension.

[0058] Preferably, the support frame 120 is provided with a transverse guide rail 121. The transverse motion seat 2111 is slidably connected to the transverse guide rail 121. Further preferably, there are two transverse guide rails 121, and the transverse motion seat 2111 is mounted on the two transverse guide rails 121.

[0059] The vertical drive device 2121 is a motor. The vertical transmission assembly 2122 includes a transmission wheel / transmission belt linkage and a screw rod / screw nut linkage (not shown in the figure). The vertical motion seat 2124 is fixedly connected to the screw rod nut of the screw rod / screw nut linkage. The vertical drive device 2121 drives the screw rod of the screw rod / screw nut linkage to rotate through the transmission wheel / transmission belt linkage, thereby driving the vertical motion seat 2124 fixedly connected to the screw rod nut to move along the screw rod extending vertically on the vertical fixed seat 2123.

[0060] Preferably, a vertical slide rail 2125 is provided on the vertical fixed seat 2123, and the vertical moving seat 2124 is slidably connected to the vertical slide rail 2125. Further preferably, there are multiple vertical slide rails 2125.

[0061] like Figure 6 and Figure 7 As shown, in a specific example, the liquid suction mechanism 220 includes a liquid suction drive device 221, a liquid suction push block 222, a liquid pipette 223 and a liquid suction head 224. The liquid suction drive device 221 is fixed on the vertical motion seat 2124. The liquid suction push block 222 is located above the liquid pipette 223 and is connected to the piston rod 2231 of the liquid pipette 223. The liquid suction drive device 221 is connected to the liquid suction push block 222 to drive the piston rod 2231 to move by driving the liquid suction push block 222 to move. The liquid suction head 224 is located below the liquid pipette 223 and is internally connected to the liquid pipette 223. The liquid suction head 224 is used to mount the suction head 20.

[0062] More specifically, the liquid suction driving device 221 is a motor. The liquid suction driving device 221 is connected to the liquid suction pushing block 222 through a screw rod / screw rod nut linkage. The liquid suction pushing block 222 is fixedly connected to the screw rod nut.

[0063] Preferably, there are multiple pipettes 223 and pipette heads 224. The piston rods 2231 of the multiple pipettes 223 are all connected to the pipette push block 222 so as to be driven to move synchronously by the pipette push block 222. The multiple pipettes 223 correspond to the multiple pipette heads 224 one by one.

[0064] In the specific example shown in the figure, the pipette head 224 is provided with a plug-pulling needle 225 for inserting through the rubber plug of the sample tube 40. The plug-pulling needle 225 is connected to the pipette 223 through the pipette head 224. After the plug-pulling needle 225 penetrates the rubber plug of the sample tube 40, air can be ventilated into the sample tube 40 through the pipette 223, so that the rubber plug is bulged to be separated from the sample tube 40.

[0065] Please continue to Figure 6 and Figure 7 In a specific example, the removal mechanism 230 includes a removal driving device 231, a removal pushing block 232, a removal pressure plate 233 and a removal pressure plate pushing shaft 234.

[0066] The withdrawal drive device 231 is arranged on the vertical motion seat 2124, and the withdrawal drive device 231 is connected to the withdrawal push block 232, and the withdrawal push block 232 is connected to the withdrawal pressure plate 233 through the withdrawal pressure plate push shaft 234. The withdrawal pressure plate 233 is sleeved on the liquid suction head 224, and the withdrawal pressure plate 233 can be driven by the withdrawal drive device 231 to move vertically along the liquid suction head 224. The bottom of the withdrawal pressure plate 233 is provided with an assembly groove 2331 surrounding the liquid suction head 224. There is a gap between the side wall of the assembly groove 2331 and the liquid suction head 224 for the suction head 20 to be inserted. When the withdrawal pressure plate 233 moves downward along the liquid suction head 224, the bottom of the assembly groove 2331 will conflict with the suction head 20 assembled in the gap, so that the suction head 20 can be withdrawn from the liquid suction head 224.

[0067] More specifically, the removal drive device 231 can be, but is not limited to, an electric push rod. Preferably, there are multiple removal drive devices 231, and the multiple removal drive devices 231 are connected to the parts near both ends of the removal push block 232 to evenly apply force to the removal push block 232.

[0068] Preferably, in the example shown in the figure, a vertically extending guide rod 2126 is provided on the vertical motion seat 2124. The guide rod 2126 passes through the liquid suction push block 222 and the withdrawal push block 232, and the liquid suction push block 222 and the withdrawal push block 232 can move along the guide rod 2126. Further preferably, there are multiple guide rods 2126 to ensure the stability of the liquid suction push block 222 and the withdrawal push block 232 in vertical movement.

[0069] Preferably, the removal drive device 231 and the liquid suction drive device 221 are respectively located on both sides of the vertical motion seat 2124 to facilitate the installation and maintenance of the structure.

[0070] Please refer to Figure 8 The above-mentioned liquid aspiration mechanism 220 with the plug-pulling needle 225 can independently constitute a pneumatic plug-pulling structure. By using the pneumatic plug-pulling structure, when it is necessary to pull the plug of a sample tube 40 with a rubber plug, the plug-pulling needle 225 can be controlled to penetrate the rubber plug, and the liquid aspiration drive device 221 can be controlled to operate. Gas is slowly injected into the sample tube 40 through the liquid aspirator 223. As the gas in the sample tube 40 gradually increases, the negative pressure in the tube gradually disappears and the air pressure gradually increases to be greater than that outside the tube. The gas in the tube will slowly separate the rubber plug from the tube body until the rubber plug and the tube body are completely separated.

[0071] Preferably, the pneumatic plug removal structure may further include the above-mentioned removal mechanism 230. When the removed plug needs to be removed, the removal drive device 231 may be controlled to drive the removal pressure plate 233 to move downward, so as to gradually push the plug on the plug removal needle 225 downward and away from the plug removal needle 225.

[0072] The pneumatic plug-pulling structure is ingeniously designed and can perform plug-pulling and removal operations fully automatically without human intervention. It has a high degree of automation and can especially avoid the risk of sample contamination or operator infection caused by human intervention, thus having high safety.

[0073] Please combine Figure 4 and Figure 5 The scanning mechanism 240 includes a scanning fixed seat 241, a scanning driving device 242, a scanning transmission component 243, a scanning positioning bracket 244 and a scanner 245. The scanning fixed seat 241 is fixed on the vertical fixed seat 2123, as in the example shown in the figure, it is fixed on the bottom of the vertical fixed seat 2123. The scanning driving device 242, the scanning transmission component 243 and the scanning positioning bracket 244 are fixed on the scanning fixed seat 241. The scanning positioning bracket 244 has a plurality of scanning windows and the plurality of scanning windows are arranged in sequence along the longitudinal direction. The scanning driving device 242 is connected to the scanner 245 through the scanning transmission component 243 to drive the scanner 245 to move along the longitudinal direction to scan the label information corresponding to different scanning windows.

[0074] More specifically, the scanning drive device 242 is a motor. The scanning transmission assembly 243 includes a transmission wheel / transmission belt linkage. The scanner 245 is fixed on the transmission belt of the transmission wheel / transmission belt linkage, so when the scanning drive device 242 drives the transmission belt to rotate through the transmission wheel, the scanner 245 can be driven to move longitudinally.

[0075] like Fig. 9 As shown, in a preferred example, the cell slide making machine 10 further includes a suction filtration device 300. The suction filtration device 300 is connected to the filter 113 and is used to extract the liquid in the filter 113.

[0076] In a specific example, the suction filtration device 300 includes a suction filtration joint 310 and a negative pressure pump 320. The negative pressure pump 320 is connected to the filter 113 through the suction filtration joint 310 to suck the liquid in the filter 113 and discharge it.

[0077] More specifically, the suction filter joint 310 is a multi-way joint. The suction filter joint 310 has a joint connected to the filter 113, a joint connected to the negative pressure pump 320, and a joint connected to the external washing liquid container 50. The washing liquid container 50 is preferably connected to the suction filter joint 310 through an electromagnetic water valve 60. In the illustrated example, there are multiple filters 113, the suction filter joint 310 is a Luer three-way joint, and there are multiple negative pressure pumps 320. Multiple filters 113, multiple joints of the suction filter joint 310 and multiple negative pressure pumps 320 are respectively connected. The negative pressure pump 320 is also connected to the external waste liquid bucket 70 through a multi-channel bus 330.

[0078] When the cell slide making machine 10 performs pre-processing of blood samples, the process mainly includes scanning, plug removal, first fixation, cell enrichment, second fixation, and gradient dehydration. The specific process of each step is described in detail in the following sections.

[0079] 1. Scan

[0080] After the "run program" instruction of the control system of the cell slide making machine 10 is started, the instrument starts to run. The lateral motion module 211 starts to run in the horizontal direction, and the lateral motion seat 2113 moves slowly to the left along the lateral transmission assembly 2112 from the right side inside the instrument, and stops just above the sample rack 115. At this time, the vertical movement seat 2124 begins to slowly descend. The lowest end of the vertical movement seat 2124 is the removal pressure plate 233. A scanning window is fixed on the right side of the removal pressure plate 233. When the positioning barcode in the scanning window is at the same height as the barcode pasted on the sample tube 40, the vertical movement seat 2124 stops descending. At this time, the scanner 245 slowly moves longitudinally from the inside of the vertical fixed seat 2123 to the outside along the scanning transmission assembly 243, and stops at the positioning barcode position on the outermost side of the scanning window. At this time, the scanning infrared light is turned on to irradiate positioning barcode No. 1, and the scanner 245 starts to return along the longitudinal direction, reading positioning barcode No. 1, sample tube barcode No. 1, positioning barcode No. 2, sample tube barcode No. 2... positioning barcode No. 6, sample tube barcode No. 6 in turn, until positioning barcode No. 7 is read, and the barcode reading is completed this time (each time a barcode is read, a "beep" sound is heard, indicating that the barcode reading is successful). At this point, the scanning of the sample tube set is complete, and the scanner 245 returns to its original position.

[0081] Then, after the vertical motion seat 2124 slowly rises to the original position, the horizontal motion seat 2113 begins to slowly move rightward in the horizontal direction to the top of the filter group and stops, and the vertical motion seat 2124 begins to slowly descend. When the positioning barcode of the scanning window and the barcode pasted on the filter 113 are at the same height, the vertical motion seat 2124 stops descending, and the scanner 245 slowly moves longitudinally from the inside of the vertical fixed seat 2123 to the outside along the scanning transmission component 243, and stops at the positioning barcode position on the outermost side of the scanning window. At this time, the scanning infrared light is turned on to irradiate the No. 1 positioning barcode, and the scanner 245 begins to return along the longitudinal direction, reading the No. 1 positioning barcode, the No. 1 filter barcode, the No. 2 positioning barcode, the No. 2 filter barcode... the No. 6 positioning barcode, the No. 6 filter barcode, until the No. 7 positioning barcode is read, and the barcode reading is completed this time (each time the barcode is read, a "beep" sound is heard, indicating that the barcode reading is successful). At this time, the filter group is scanned and the scanner 245 returns to its original position.

[0082] At this point, the entire scanning process is completed. The control system of the cell preparation machine 10 will analyze whether the sample tube group barcode and the filter group barcode information correspond one to one, and then make a process. If the information does not correspond, the instrument will issue an alarm. If the barcode information corresponds correctly, the instrument will continue to operate normally and then proceed to the next step.

[0083] 2. Unplug

[0084] After the filter 113 is scanned and the barcode information is correct, the vertical motion seat 2124 rises to its original position, and the horizontal motion seat 2113 moves horizontally to the top of the sample rack 115, and the pressure plate 233 is removed and retracted into the vertical fixed seat 2123, exposing the pipette head 224 and the plug removal needle 225. The vertical motion seat 2124 begins to move down slowly until the end of the pipette head bottom 224 is about 0.5 mm away from the surface of the rubber plug of the sample tube 40, and the plug removal needle 225 has penetrated into the rubber plug. The piston rod 2231 inside the pipette 223 slowly moves downward, and the gas inside the pipette 223 enters into the sample tube 40 along the plug-pulling needle 225 to balance the negative pressure inside the sample tube 40. When the pressure inside the sample tube 40 is higher than the external pressure, the rubber stopper will gradually be pushed out and separated from the sample tube 40, but it is still fixed on the plug-pulling needle 225. The vertical motion seat 2124 slowly moves upward, and the pulled out rubber stopper is attached to the plug-pulling needle 225 and moves upward together (during the entire operation of the instrument, the sample tube fixing device is always in the fixed sample tube, so even if the bottle cork is not pulled out during the plug-pulling, the sample tube 40 will not move upward with the vertical operation). After the vertical motion seat 2124 moves up to its original position, the horizontal motion seat 2113 will move horizontally to the left to just above the waste tank 116. At this time, the removal pressure plate 233 is driven to press down to remove the rubber plug on the plug-pulling needle 225 into the waste tank 116. The removal pressure plate 233 will repeat the pressure relief twice to ensure that the rubber plug on the plug-pulling needle 225 is completely removed (there is an infrared sensing light detection mechanism at the rubber plug position of the sample tube group. If the rubber plug of the sample tube 40 is not removed, the sensor will sense it, and the instrument will perform the plug removal work again until the rubber plug is completely removed. The number of plug removal times for a single operation is set to 3 times. If the plug removal is not complete after 3 times, the instrument will alarm). After removing the plug, the vertical motion seat 2124 returns to the origin and starts the next step.

[0085] 3. First fixation (fixation in sample tube)

[0086] (1) Wastewater removal

[0087] After the plug is removed, the vertical motion seat 2124 returns to the origin, and the lateral motion seat 2113 moves horizontally to the right to the top of the tip rack 111A (the tip rack 111 between the reagent strip slot 114 and the filter 113). After the pressure plate 233 is retracted into the vertical fixed seat 2123 to expose the liquid pipetting head 224, the vertical motion seat 2124 moves downward until the liquid pipetting head 224 penetrates the upper end of the tip and stops. At this time, the tip is attached to the liquid pipetting head 224. The vertical motion seat 2124 returns to the original position, and the tip 20 on the tip rack 111A is lifted up by the liquid pipetting head 224. The lateral motion seat 2123 moves to the top of the sample tube 40 and stops. The piston rod 2231 in the pipette 223 first moves vertically downward to empty the control gas in the pipette 223, and then the vertical motion seat 2124 slowly moves downward to the bottom of the suction head and moves downward to the inside of the sample tube 40 about 3 cm away from the sample tube mouth and stops. The piston in the pipette 223 starts to move upward slowly, and the supernatant blood in the sample tube is slowly sucked into the suction head 20, and stops after sucking about 4 ml of supernatant blood. After moving vertically upward for a distance, the suction head 20 is pulled out of the sample tube 40, and the horizontal motion seat 2113 slowly moves horizontally to the left to the top of the waste tank 116. The piston rod 2231 of the pipette 223 slowly moves downward to discharge the liquid in the suction head 20, and flows into the waste tank 116 and flows into the waste liquid collection bucket along the pipeline connected to the waste tank 116 in the base. Then the horizontal motion seat 2113 moves again to the top of the sample tube 40, and sucks the same supernatant blood for the second time in the same way as above, and then removes it. This process is repeated 5 times in total, and each time the blood sampling position of the pipette tip 20 is lower by a fixed distance than the previous time, until the supernatant blood in the sample tube 40 is almost completely collected (about 1 ml of supernatant blood is left at the bottom of the sample tube 40, and the cell sediment at the bottom of the sample tube 40 is required for the experiment, so the supernatant blood cannot be removed completely).

[0088] (2) Rinse the suction tip

[0089] After the last discharge of the supernatant serum waste liquid, the vertical motion seat 2124 returns to the origin, the horizontal motion seat 2113 moves to the top of the reagent strip slot 114A, the piston rod 2231 of the pipette 223 moves downward to discharge the air in the pipette 223, the vertical motion seat 2124 moves downward, and the bottom end of the suction head stops 3 to 5 mm from the bottom end of the reagent strip slot 114A. The piston rod 2231 inside the pipette 223 starts to move upward to slowly absorb about 4 ml of liquid into the suction head 20, and then the piston rod 2231 slowly descends, and the liquid is discharged into the reagent strip slot 114A again, and this is repeated 5 times (the blood stains remaining in the suction head will be rinsed clean). During the fifth rinse, after about 4 ml of liquid is sucked into the suction head, the piston rod 2231 stops descending, the vertical motion seat 2124 rises back to the origin, and the horizontal motion seat 2113 runs just above the waste tank 116 to move the waste liquid in the suction head into the waste tank 116.

[0090] (3) Add fixative

[0091] After draining the waste liquid, the horizontal motion seat 2113 moves to the top of the reagent strip slot 114B (located adjacent to the left side of the reagent strip slot 1114A), the piston of the liquid pipette 223 moves downward to discharge the air in the liquid pipette 223, and the vertical motion seat 2124 moves downward, and the bottom end of the suction head stops at about 3 to 5 mm from the bottom end of the reagent strip slot 114B. The piston rod 2231 inside the liquid pipette 223 starts to move upward and slowly sucks about 4 ml of liquid into the suction head. The vertical motion seat 2124 moves up to the origin, the horizontal motion seat 2113 moves to the top of the sample tube 40 and stops, the vertical motion seat 2124 slowly moves down to the inside of the sample and stops, and the liquid in the suction head 20 is drained into the sample tube 40, and then the vertical motion seat 2124 moves down again to the bottom end of the suction head 20 and stops at about 1 cm from the bottom of the sample tube 40. The suction head 20 starts to repeatedly suck and beat the liquid 5 times (mixing the cell sediment). After mixing, the vertical motion seat 2124 moves up to the bottom of the suction head 20 and stops when it is flush with the mouth of the sample tube 40. At this time, the first fixation begins. In this state, it remains stationary for 8 minutes.

[0092] 4. Cell Enrichment

[0093] After the fixed time of 8 minutes, the vertical motion seat 2124 moves down to the bottom of the suction head 20 and stops when it is about 3 to 5 mm away from the mouth of the sample tube 40. The aspirator 223 sucks all the liquid in the sample tube 40 into the suction head 20. The vertical motion seat 2124 moves up to the original position, the horizontal motion seat 2113 moves to the right above the filter group, and the vertical motion seat 2124 moves down to the bottom of the suction head 20 and stops flush with the mouth of the filter 113, and the liquid in the suction head 20 is discharged into the filter 113. The negative pressure pump 320 is turned on, and the liquid in the filter 113 is filtered out and flows along the waste liquid pipeline into the waste liquid barrel, and the cells are enriched on the filter membrane in the filter 113. After the negative pressure is turned on for 15 seconds, the water supply valve is opened, and the cleaning liquid flushes the filter head along the cleaning pipeline, and the waste liquid also flows into the waste liquid barrel. After flushing for about 7 seconds, the negative pressure pump 320 and the water valve are closed and the entire suction filtration process ends. The vertical motion seat 2124 moves down to the bottom of the suction head 20 and stops at a position about 3 mm above the inner membrane of the filter 113. The piston rod 2231 of the pipette 223 moves up to absorb the liquid that has not been filtered. The vertical motion seat 2124 moves up to the origin, the horizontal motion seat 2113 moves to the top of the sample tube 40, and the vertical motion seat 2124 moves down to the inside of the sample tube 40 to pump the liquid in the suction head 20 into the sample tube 40 (generally, there will be no liquid in the suction head 20 at this step, and the filter 113 is rarely blocked).

[0094] The vertical motion seat 2124 is reset, and the lateral motion seat 2113 stops after running to the top of the reagent strip groove 114C. The piston of the liquid pipette 223 moves downward to discharge the air in the liquid pipette 223. The vertical motion seat 2124 moves downward, and the bottom end of the suction head 20 stops at 3 to 5 mm from the bottom end of the reagent strip groove 114C. After sucking about 4 ml of liquid, the vertical motion seat 2124 is reset, and the lateral motion seat 2113 runs to the top of the waste groove 116, and the liquid is discharged into the waste groove 116 (this step is to clean the pipeline of the waste groove 116). Then the lateral motion seat 2113 runs to the top of the waste groove 116 and stops. At this time, the pressure plate 233 is slowly moved downward, and the suction head 20 is removed and dropped into the waste groove 116. Then the vertical motion seat 2124 is reset.

[0095] 5. Second fixation of cells (fixation inside filter 113)

[0096] (1) Add fixative

[0097] The lateral motion seat 2113 moves to the top of the tip rack 20B (the tip rack 20B is located on the right side of the tip rack 20A and is adjacent to the reagent tank 112). After the pressure plate 233 is removed and moved up to expose the liquid pipetting head 224, the vertical motion seat 2124 slowly moves down. After the tip 20 is inserted, the vertical motion seat 2124 is reset. The lateral motion seat 2113 moves to the left to the top of the reagent tank 112A. The vertical motion seat 2124 moves down to the bottom of the tip 20 about 5 mm from the bottom of the tank and stops. It is reset after absorbing about 2 ml of liquid. The lateral motion seat 2113 runs to the top of the filter 113. The vertical motion seat 2124 moves down to the bottom of the tip 20 and is flush with the filter 113. After the liquid is added to the filter 113, the second fixation begins. The fixation time is 60 minutes. The instrument is stationary in this state during the fixation time.

[0098] (2) Filtration

[0099] After 60 minutes, the negative pressure pump 320 is turned on. After 15 seconds of the negative pressure being turned on, the water supply valve is opened, and the cleaning liquid flushes the filter head along the cleaning pipeline. The waste liquid also flows into the waste liquid bucket. After flushing for about 7 seconds, the negative pressure pump 320 and the water valve are closed, and the entire filtration process is completed. The vertical motion seat 2124 moves down to the bottom of the suction head 20 and stops about 3 mm from the inner membrane of the filter 113. The piston rod 2231 of the aspirator 223 moves up to absorb the liquid that has not been filtered. The vertical motion seat 2124 moves up to the origin, the horizontal motion seat 2113 runs to the top of the sample tube 40, and the vertical motion seat 2124 moves down to the inside of the sample tube 40 to pump the liquid in the suction head 20 into the sample tube 40 (generally, there will be no liquid in the suction head 20 at this step, and the filter 113 rarely gets clogged).

[0100] 6. Gradient dehydration

[0101] (1) Gradient dehydration

[0102] The horizontal motion seat 2113 moves to the position just above the reagent tank 112B (to the right of the reagent tank 112A) and stops. The vertical motion seat 2124 moves down to the position about 5 mm above the bottom of the reagent tank 112B where the lower end of the suction head 20 stops. After sucking about 1 ml of solution, the vertical motion seat 2124 resets. The horizontal motion seat 2113 moves to the position just above the filter 113. The vertical motion seat 2124 moves down to the nozzle of the filter 113 and stops, and the liquid is discharged into the filter 113. After waiting for 2 minutes, the negative pressure pump 320 is turned on. After 15 seconds of negative pressure, the water supply valve is opened, and the cleaning liquid flushes the filter head along the cleaning pipeline. The waste liquid also flows into the waste liquid barrel. After flushing for about 7 seconds, the negative pressure pump 320 and the water valve are closed, and the entire filtration process ends. The vertical motion seat 2124 moves down to the bottom of the suction head 20 and stops at a position about 3 mm above the inner membrane of the filter 113. The piston rod 2231 of the pipette 223 moves up to absorb the liquid that has not been filtered. The vertical motion seat 2124 moves up to the origin, the horizontal motion seat 2113 moves to the top of the sample tube 40, and the vertical motion seat 2124 moves down to the inside of the sample tube 40 to pump the liquid in the suction head 20 into the sample tube 40 (generally, there will be no liquid in the suction head 20 at this step, and the filter 113 is rarely blocked).

[0103] Then the transverse motion seat 2113 moves the reagent tank 112C to repeat the same action as the reagent tank 112B, and the same is true for the reagent tank 112D. So far, the gradient dehydration (three dehydration) is completed.

[0104] (2) Cleaning

[0105] After dehydration is completed, the horizontal motion seat 2113 moves to the reagent tank 112E slot to absorb about 4 ml of liquid, and stops above the waste tank 116. When the vertical motion seat 2124 moves down to the bottom of the suction head 20 and is flush with the opening of the waste tank 116, the liquid is discharged to flush the pipeline. This action is repeated twice. After two washes, the suction head 20 is discharged into the waste tank 116, and the horizontal motion seat 2113 moves to the farthest edge of the instrument. At this point, the entire film production process ends.

[0106] The cell slide making machine 10 can automatically collect cells without too much manual operation, and the collection rate and stable maintenance of rare cells such as CTC are significantly improved. The cell slide making machine can automatically identify the label information on the sample and the filter, and can avoid the mistakes that may be caused by manual operation. The cell slide making machine can be used to collect rare cells, and automatic operations can be realized from scanning, plug removal, liquid addition, cell fixation, filtration collection, dehydration after fixation, etc., and the sample processing process can be completed at one time, reducing the manual processing time, saving a lot of experimental time, and significantly improving the collection efficiency.

[0107] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0108] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A cell slice making machine, characterized in that: include: A rack, comprising a base and a support frame mounted on the base, wherein the base is provided with a plurality of placement positions, and the plurality of placement positions are respectively used to place a tip rack, a reagent tank, a filter, a reagent strip tank, a sample rack and a waste tank; as well as a liquid transfer device, mounted on the support frame and located above the placement position, the liquid transfer device comprising a position adjustment mechanism, a liquid suction mechanism, a removal mechanism and a scanning mechanism, the liquid suction mechanism, the removal mechanism and the scanning mechanism being connected to the position adjustment mechanism and being driven by the position adjustment mechanism to move between different placement positions, the liquid suction mechanism being used to load a tip from the tip rack to suck the corresponding liquid into the filter or discharge it from the waste tank, the removal mechanism being used to remove the tip installed on the liquid suction mechanism and the rubber plug on the sample tube to the waste tank, and the scanning mechanism being used to scan the labels of the sample tube and the filter on the sample rack; The position adjustment mechanism includes a lateral motion module and a vertical motion module; the lateral motion module includes a lateral drive device, a lateral transmission assembly and a lateral motion seat; the vertical motion module includes a vertical drive device, a vertical transmission assembly, a vertical fixed seat and a vertical motion seat; the lateral motion seat is movably arranged on the support frame and can move in the lateral direction, the vertical fixed seat is fixed on the lateral motion seat, the vertical motion seat is movably arranged on the vertical fixed seat and can move in the vertical direction, and the liquid suction mechanism and the removal mechanism are arranged on the vertical motion seat; The transverse driving device is fixed on the support frame, and the transverse driving device is connected to the transverse motion seat through the transverse transmission assembly to drive the transverse motion seat to move and drive the vertical fixed seat to move in the transverse direction; The vertical driving device is fixed on the lateral motion seat or the vertical fixed seat, and the vertical driving device is connected to the vertical motion seat through the vertical transmission assembly to drive the liquid suction mechanism and the removal mechanism to move vertically by driving the vertical motion seat to move; The liquid suction mechanism comprises a liquid suction driving device, a liquid suction pushing block, a liquid suction device and a liquid suction head; The liquid suction driving device is arranged on the vertical motion seat, the liquid suction pushing block is located above the liquid suction device and is connected to the piston rod of the liquid suction device, the liquid suction driving device is connected to the liquid suction pushing block to drive the piston rod to move by driving the liquid suction pushing block to move, the liquid suction head is located below the liquid suction device and is internally connected to the liquid suction device, and the liquid suction head is used to install the suction head; There are a plurality of pipette tip racks, and the plurality of pipette tip racks are located at different positions of the base, so that the pipette device can conveniently load and remove pipette tips at a plurality of different positions.

2. The cell slide making machine according to claim 1, characterized in that: The transverse driving device is a motor, the transverse transmission assembly includes a transmission wheel and / or a transmission belt linkage and a screw rod and / or a screw rod nut linkage, the transverse motion seat is fixedly connected to the screw rod and / or the screw rod nut of the screw rod and / or the screw rod nut linkage, and the transverse driving device drives the screw rod of the screw rod and / or the screw rod nut linkage to rotate through the transmission wheel and / or the transmission belt linkage, thereby driving the transverse motion seat to move along the screw rod extending laterally; The vertical driving device is a motor, and the vertical transmission assembly includes a transmission wheel and / or a transmission belt linkage and a screw rod and / or a screw rod nut linkage. The vertical motion seat is fixedly connected to the screw rod nut of the screw rod and / or the screw rod nut linkage. The vertical driving device drives the screw rod of the screw rod and / or the screw rod nut linkage to rotate through the transmission wheel and / or the transmission belt linkage, thereby driving the vertical motion seat to move along the screw rod extending vertically.

3. The cell slide making machine according to claim 2, characterized in that: The support frame is provided with a transverse slide rail, and the transverse motion seat is slidably connected to the transverse slide rail; the vertical fixed seat is provided with a vertical slide rail, and the vertical motion seat is slidably connected to the vertical slide rail.

4. The cell slice preparation machine according to claim 3, characterized in that: The liquid suction drive device is a motor, and the liquid suction drive device is connected to the liquid suction push block through a screw and / or a screw nut of a screw nut linkage; and / or There are multiple pipettes and pipette heads, the piston rods of the multiple pipettes are connected to the pipette push block to be driven by the pipette push block to move synchronously, and the multiple pipettes correspond to the multiple pipette heads one by one; and / or A plug-pulling needle for inserting through the rubber plug of the sample tube is provided below the liquid-pipette head, and the plug-pulling needle is connected with the liquid-pipette device through the liquid-pipette head.

5. The cell slide making machine according to claim 4, characterized in that: The stripping mechanism comprises a stripping drive device, a stripping push block, a stripping pressure plate and a stripping pressure plate push shaft; The withdrawal drive device is arranged on the vertical motion seat, the withdrawal drive device is connected to the withdrawal push block, the withdrawal push block is connected to the withdrawal pressure plate through the withdrawal pressure plate push shaft, the withdrawal pressure plate is sleeved on the liquid suction head, the withdrawal pressure plate can be driven by the withdrawal drive device to move vertically along the liquid suction head, the bottom of the withdrawal pressure plate is provided with an assembly groove surrounding the liquid suction head, and there is a gap between the side wall of the assembly groove and the liquid suction head for the suction head to be inserted.

6. The cell slide making machine according to claim 5, characterized in that: The removal drive device is an electric push rod; and / or There are multiple removal drive devices; and / or A vertically extending guide rod is provided on the vertical motion seat, the guide rod passes through the liquid suction push block and the removal push block, and the liquid suction push block and the removal push block can move along the guide rod; and / or The removal driving device and the liquid suction driving device are respectively located on two sides of the vertical motion seat.

7. The cell slice preparation machine according to any one of claims 1 to 5, characterized in that: The scanning mechanism includes a scanning fixed seat, a scanning driving device, a scanning transmission component, a scanning positioning bracket and a scanner; The scanning fixed seat is fixed on the vertical fixed seat, the scanning drive device, the scanning transmission assembly and the scanning positioning bracket are fixed on the scanning fixed seat, the scanning positioning bracket has a plurality of scanning windows and the plurality of scanning windows are arranged in sequence along the longitudinal direction, the scanning drive device is connected to the scanner through the scanning transmission assembly to drive the scanner to move along the longitudinal direction to scan the label information corresponding to different scanning windows.

8. The cell slice preparation machine according to any one of claims 1 to 5, characterized in that: Also includes a filtration device; The suction filtration device comprises a suction filtration joint and a negative pressure pump, and the negative pressure pump is connected to the filter through the suction filtration joint to suck the liquid in the filter and discharge it.

9. The cell slide making machine according to claim 8, characterized in that: The suction filtration joint is a multi-way joint, and the suction filtration joint has a joint connected to the filter, a joint connected to the negative pressure pump, and a joint connected to an external washing liquid container.

10. The cell slide making machine according to claim 9, characterized in that: There are multiple filters, the suction filter connector is a Luer three-way connector, there are multiple negative pressure pumps, and the multiple filters, multiple connectors of the suction filter connector and multiple negative pressure pumps are connected correspondingly.

11. The cell preparation machine according to any one of claims 1 to 7 and 9 to 10, characterized in that: There are multiple reagent tanks; and / or the waste tank includes a waste tank and a waste material tank.

12. The cell preparation machine according to any one of claims 1 to 7 and 9 to 10, characterized in that: It also includes a sample fixing mechanism; the sample fixing mechanism is arranged below the base, the base is hollowed out corresponding to the placement position of the sample rack, the sample tube can pass through the base and extend into the sample fixing mechanism, and the sample fixing mechanism is used to fix the sample tube on the sample rack.

13. The cell slide making machine according to claim 12, characterized in that: The sample fixing mechanism includes an electric cylinder driving device, a fixing spring and a fixing block; the electric cylinder driving device is connected to the fixing block via the fixing spring, the sample tube can extend between the fixing block and the electric cylinder driving device, and the electric cylinder driving device can clamp the sample tube and limit it on the sample rack via the fixing block.

Images