An automatic constant temperature water replenishing device for stem cell culture process

Abstract

The present invention discloses an automatic constant temperature water replenishment device for use in a stem cell culture process, comprising a water bath, a water replenishment system, a water level detection device and a controller. In the water replenishment system, water in a hot water pipe is heated by a heating unit and flows into an automatic thermostatic valve together with cold water in a cold water pipe; the automatic thermostatic valve ensures that the temperature of the water flowing out is at a set temperature. The water flowing out of the automatic thermostatic valve flows into a confluence pipe and then into an automatic reflux three-way valve, and the two output ends of the automatic reflux three-way valve are respectively connected to the output pipe and the reflux pipe. A temperature probe is provided at a position near the output end of the confluence pipe, and the temperature probe is used to detect whether the water temperature at the output end of the confluence pipe is at a set temperature. If so, the water flows into the output pipe, otherwise, the water flows into the reflux pipe and re-enters the hot water pipe. The water in the output pipe finally flows into the water bath. The water level detection device in the water bath and the solenoid valve on the output pipe cooperate to control whether the water bath is replenished with water. The controller is the control center of the present invention.

Description

Technical Field

[0001] The invention belongs to the field of experimental instruments, and in particular relates to an automatic constant-temperature water replenishing device used in a stem cell culture process. Background Art

[0002] Generally, during stem cell culture, reagents, culture fluids, digestive enzymes, etc. need to be preheated at a certain constant temperature to ensure experimental stability and cell culture experiments, and the commonly used heating device is a water bath.

[0003] Existing water baths are generally used after adding an appropriate amount of water before use and then heated to a predetermined temperature. However, when the constant temperature heating time is long or needs to be used continuously, the water in the water bath will gradually decrease due to evaporation, causing the water level to drop, affecting the experimental results, so it is necessary to replenish water in time.

[0004] The existing water bath automatic constant temperature water replenishment only heats the water to be heated to the specified temperature and then directly flows into the water bath. However, the water will pass through a pipe at one end when flowing from the heating point to the water bath, and the water will dissipate heat in this pipe, so the water may not be at the set temperature when it enters the water bath. Summary of the invention

[0005] In order to solve the above problems, the technical problem to be solved by the present invention is to provide an automatic constant temperature water replenishing device for use in the stem cell culture process, which is used to solve the problem of large water temperature fluctuations in the existing constant temperature water adding device.

[0006] The technical solution of the present invention is:

[0007] An automatic constant temperature water replenishing device for use in a stem cell culture process, characterized in that it comprises a water bath, a water replenishing system, a water level detection device and a controller;

[0008] At least one water inlet and a corresponding diversion pipe are provided on the periphery of the water bath, and the setting height of the water inlet is lower than the minimum limit water level height of the water bath, and each of the water inlets is connected to the output end of the corresponding diversion pipe; the water level detection device is arranged in the water bath;

[0009] The water replenishment system includes a cold water pipeline, a hot water pipeline, a confluence pipeline, a return pipeline and an output pipeline, and also includes:

[0010] An automatic thermostatic valve, wherein a first input end of the automatic thermostatic valve is communicated with an output end of the cold water pipe, a second input end of the automatic thermostatic valve is communicated with an output end of the hot water pipe, and an output end of the automatic thermostatic valve is communicated with an input end of the converging pipe; an input end of the cold water pipe and an input end of the hot water pipe are both communicated with an external water pipe, a heating section is provided on the hot water pipe, and a heating portion is provided on the heating section;

[0011] An automatic reflux three-way valve, wherein the input end of the automatic reflux three-way valve is connected to the output end of the merging pipe, and the first output end of the automatic reflux three-way valve is connected to the input end of the output pipe; the second output end of the automatic reflux three-way valve is connected to the input end of the reflux pipe, and the output end of the reflux pipe is connected to the hot water pipe; a temperature probe is provided on the output end of the merging pipe;

[0012] The output end of the output pipeline is the main water inlet, and the input ends of all the branch pipelines are connected to the main water inlet; a solenoid valve is provided on the output pipeline near the automatic reflux three-way valve;

[0013] The water level detection device, the heating unit, the temperature probe, the automatic reflux three-way valve and the solenoid valve are respectively connected to the controller with electrical signals;

[0014] When the water level detection device detects that the water level in the water bath is lower than the set minimum water level, the controller controls the solenoid valve to open; after the water level detection device detects that the water level in the water bath is higher than the set maximum water level, the controller controls the solenoid valve to close;

[0015] When the temperature probe detects that the temperature of the merging pipe meets the set temperature, the controller controls the first output end of the automatic reflux three-way valve to open and the second output end to close; when the temperature probe detects that the temperature of the merging pipe deviates from the set temperature, the controller controls the first output end of the automatic reflux three-way valve to close and the second output end to open.

[0016] In one embodiment of the present invention, the heating section is a spiral pipe, and the heating part is attached to the spiral pipe.

[0017] In one embodiment of the present invention, the automatic thermostatic valve is provided with a temperature controller and a flow controller, the temperature controller is used to set and control the temperature of the water flow output from the output end of the automatic thermostatic valve, and the flow controller is used to control the size of the water flow output from the output end of the automatic thermostatic valve.

[0018] In one embodiment of the present invention, a one-way water stop valve is provided on the return pipe.

[0019] In one embodiment of the present invention, each of the diversion pipes is provided with a one-way water stop valve.

[0020] In one embodiment of the present invention, a first filter is provided at the output end of the cold water pipe, and a second filter is provided at the output end of the hot water pipe.

[0021] In one embodiment of the present invention, each of the water inlets is provided with a partition plate, and a plurality of through holes are opened on the partition plate.

[0022] In one embodiment of the present invention, the number of the water inlets is four or six, and the water inlets are evenly distributed around the water bath.

[0023] In one embodiment of the present invention, the total water inlet is arranged at a height higher than the maximum limit water level of the water bath.

[0024] In one embodiment of the present invention, it also includes a temperature detection device, an alarm indicator light and a communication module; the temperature detection device is arranged in the water bath, and the setting height of the temperature detection device is lower than the minimum limit water level height of the water bath; the temperature detection device, the alarm indicator light and the communication module are respectively connected to the controller with electrical signals.

[0025] Due to the adoption of the above technical solution, the present invention has the following advantages and positive effects compared with the prior art:

[0026] (1) The water in the hot water pipe is heated by the heating unit to become hot water, and the cold water in the cold water pipe flows into the automatic thermostatic valve respectively. The automatic thermostatic valve is provided with a specified temperature range. When the temperature of the mixed hot and cold water input reaches the specified temperature range, it flows into the confluence pipe. In order to prevent the water temperature from changing beyond the specified temperature range in the confluence pipe, a temperature probe is provided at the output end of the confluence pipe. When the temperature probe detects that the water temperature in the confluence pipe is within the specified temperature range, the automatic reflux three-way valve opens the switch leading to the output pipe and closes the switch leading to the reflux pipe, and the constant temperature water is output to the output pipe; when the water temperature deviates from the specified temperature range, the automatic reflux three-way valve opens the switch leading to the reflux pipe and closes the switch leading to the output pipe, so that the water whose temperature does not reach the specified temperature range flows back to the hot water pipe through the reflux pipe and flows through the thermostatic valve again. Among them, the water reaching the specified temperature passes through the output pipe, from the total water inlet of the output pipe through the diversion pipe, and enters the water bath from the water inlet. The embodiment of the present invention has the function of constant temperature water replenishment, that is, the temperature of the water replenished from the outside into the water bath is always the set temperature, achieving the effect of accurately controlling the temperature of the added constant temperature water, and solving the problem of large water temperature fluctuations in the existing constant temperature water adding device.

[0027] (2) There is a water level detection device in the water bath. When the water level in the water bath is lower than the minimum limit water level, the solenoid valve is opened and the water replenishment system replenishes water to the water bath. When the water level in the water bath is higher than the maximum limit water level, the solenoid valve is closed and the water replenishment system stops replenishing water to the water bath. This enables the embodiment of the present invention to achieve the function of automatic water replenishment.

[0028] (3) The height of the water bath inlet is set lower than the minimum water level of the water bath, which is a gentle means to mix the added water with the water in the water bath. If the height of the water bath inlet is equal to or higher than the minimum water level of the water bath, the added water will mix with the water in the water bath in a manner similar to a waterfall or rain, causing the originally calm water in the water bath to vibrate and agitate, destroying the water environment in the water bath. At the same time, the height of the water bath inlet is lower than the minimum water level of the water bath, so that when adding water, the water temperature is less affected by the external environment and ensures constant temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The specific embodiments of the present invention are further described in detail below with reference to the accompanying drawings, wherein:

[0030] Figure 1 This is a schematic structural diagram of an automatic water replenishment device for stem cell culture process of the present invention;

[0031] Figure 2 A schematic diagram of a water inlet baffle of an automatic water replenishment device used in a stem cell culture process;

[0032] Figure 3 This is a schematic diagram of an operation display platform of an automatic water replenishment device used in a stem cell culture process.

[0033] Description of reference numerals:

[0034] 1: Water bath; 2: Diversion pipe; 3: Cold water pipe; 4: Hot water pipe; 5: Junction pipe; 6: Reflux pipe; 7: Output pipe; 8: Automatic thermostatic valve; 9: Heating section; 10: Automatic reflux three-way valve; 11: Temperature probe; 12: Main water inlet; 13: Solenoid valve; 14: One-way water stop valve; 15: Output temperature display screen; 16: Temperature adjustment knob; 17: Flow adjustment knob; 18: Water inlet; 19: Partition; 20: Through hole; 21: Operation display platform; 22: Operation input port: 23: Water level display; 24: Actual temperature display; 25: Set temperature display; 26: Alarm indicator light. DETAILED DESCRIPTION

[0035] The following is a further detailed description of an automatic constant temperature water replenishing device for stem cell culture proposed by the present invention in combination with the accompanying drawings and specific embodiments. The advantages and features of the present invention will become clearer according to the following description and claims. It should be noted that the accompanying drawings are in a very simplified form and use non-precise ratios, which are only used to conveniently and clearly assist in explaining the purpose of the embodiments of the present invention.

[0036] See also Figure 1In one embodiment, an automatic constant temperature water replenishment device for stem cell culture includes a water bath 1, a water replenishment system, a water level detection device and a controller. At least one water inlet 18 and a corresponding shunt pipe 2 are provided on the circumference of the water bath 1, and the setting height of the water inlet 18 is lower than the minimum limit water level of the water bath 1, and each water inlet 18 is connected to the output end of the corresponding shunt pipe 2; the water level detection device is arranged in the water bath 1. The water replenishment system includes a cold water pipe 3, a hot water pipe 4, a confluence pipe 5, a reflux pipe 6 and an output pipe 7, and also includes an automatic thermostatic valve 8, an automatic reflux three-way valve 10 and a solenoid valve 13. The first input end of the automatic thermostatic valve 8 is connected to the output end of the cold water pipe 3, the second input end of the automatic thermostatic valve 8 is connected to the output end of the hot water pipe 4, and the output end of the automatic thermostatic valve 8 is connected to the input end of the confluence pipe 5. The input end of the cold water pipe 3 and the input end of the hot water pipe 4 are both connected to the external water pipe, and the hot water pipe 4 is provided with a heating section 9, and the heating section 9 is provided with a heating part. An automatic reflux three-way valve 10, the input end of the automatic reflux three-way valve 10 is connected to the output end of the confluence pipe 5, and the first output end of the automatic reflux three-way valve 10 is connected to the input end of the output pipe 7. The second output end of the automatic reflux three-way valve 10 is connected to the input end of the reflux pipe 6, and the output end of the reflux pipe 6 is connected to the hot water pipe 4. A temperature probe 11 is provided on the output end of the confluence pipe 5. The output end of the output pipe 7 is a total water inlet 12, and the input ends of the diversion pipes 2 are all connected to the total water inlet 12. A solenoid valve 13 is provided on the output pipe 7 near the automatic reflux three-way valve 10. The water level detection device, the heating part, the temperature probe 11, the automatic reflux three-way valve 10 and the solenoid valve 13 are respectively connected to the controller electrical signal. When the water level detection device detects that the water level in the water bath 1 is lower than the set minimum water level, the controller controls the solenoid valve 13 to open, and after the water level detection device detects that the water level in the water bath 1 is higher than the set maximum water level, the controller controls the solenoid valve 13 to close. When the temperature probe 11 detects that the temperature of the converging pipe 5 meets the set temperature, the controller controls the first output end of the automatic reflux three-way valve 10 to open and the second output end to close. When the temperature probe 11 detects that the temperature of the converging pipe 5 deviates from the set temperature, the controller controls the first output end of the automatic reflux three-way valve 10 to close and the second output end to open.

[0037] In this embodiment, the water in the hot water pipe 4 is heated by the heating unit to become hot water, and the cold water in the cold water pipe 3 flows into the automatic thermostatic valve 8 respectively. The automatic thermostatic valve 8 is provided with a specified temperature range. When the temperature of the mixed hot water and cold water input reaches the specified temperature range, it flows into the converging pipe 5. In order to prevent the water temperature from changing beyond the specified temperature range in the converging pipe 5, a temperature probe 11 is provided at the output end of the converging pipe 5. When the temperature probe 11 detects that the water temperature in the converging pipe 5 is within the specified temperature range, the automatic reflux three-way valve 10 opens the switch leading to the output pipe 7 and closes the switch leading to the reflux pipe 6, and the constant temperature water is output to the output pipe 7; when the water temperature deviates from the specified temperature range, the automatic reflux three-way valve 10 opens the switch leading to the reflux pipe 6 and closes the switch leading to the output pipe 7, so that the water whose temperature does not reach the specified temperature range flows back to the hot water pipe 4 through the reflux pipe 6 and flows through the thermostatic valve again. The water reaching the specified temperature passes through the output pipe 7, and enters the water bath 1 from the total water inlet 12 of the output pipe 7 through the diversion pipe 2 and the water inlet 18. The embodiment of the present invention has the function of constant temperature water replenishment, that is, the temperature of the water replenished from the outside into the water bath 1 is always the set temperature, and the effect of accurately controlling the temperature of the added constant temperature water is achieved, which solves the problem of large fluctuation of the water temperature of the added water in the existing constant temperature water adding device.

[0038] This embodiment can also automatically replenish water. There is a water level detection device in the water bath 1. When the water level in the water bath 1 is lower than the minimum limit water level, the solenoid valve 13 is opened, and the water replenishment system replenishes water to the water bath 1. When the water level in the water bath 1 is higher than the maximum limit water level, the solenoid valve 13 is closed, and the water replenishment system stops replenishing water to the water bath 1. Among them, the minimum limit water level and the minimum limit water level are determined according to the water level required in the experiment.

[0039] In the embodiment, the height of the water inlet 18 of the water bath 1 is set lower than the minimum water level of the water bath 1, which is a gentle means to mix the supplemented water with the water in the water bath 1. If the height of the water inlet is equal to or higher than the minimum water level of the water bath 1, the supplemented water will mix with the water in the water bath 1 in a manner similar to a waterfall or rain, causing the originally calm water in the water bath 1 to vibrate and agitate, destroying the water environment for stem cell culture in the water bath 1. At the same time, the height of the water inlet 18 of the water bath 1 is lower than the minimum water level of the water bath 1, so that when replenishing water, the water temperature is less affected by the external environment and a constant temperature is ensured.

[0040] This embodiment is used for stem cell culture experiments and is a heating device in the experiment. According to the characteristics of stem cells, the temperature of the heating device needs to be set to a temperature suitable for the survival of stem cells, that is, 37 degrees. In this embodiment, the heating device is a water bath 1. At the same time, this embodiment is also equipped with a water replenishment system and some control detection devices to ensure that this embodiment can automatically detect and add water that meets the temperature setting when the water bath 1 is short of water. Therefore, the temperature of the water bath 1 is set to 37 degrees, and the temperature of the water added from the outside must also be 37 degrees.

[0041] In this embodiment, the water level detection device will detect the water level signal in the water bath 1 and send it to the controller. The controller determines that if the water level in the water bath 1 is lower than the minimum limit water level, the controller sends a signal to the solenoid valve 13 and the heating unit, the solenoid valve 13 opens, and the heating unit starts to heat the heating section 9. Afterwards, if the controller receives the water level signal and determines that the water level in the water bath 1 reaches the maximum limit water level gauge, the controller sends a signal to the solenoid valve 13 and the heating unit, the solenoid valve 13 closes, and the heating unit stops heating. In this process, the water level detection device can be an infrared sensing device. A low water level infrared sensing device is set at the minimum limit water level in the water bath 1, and a high water level infrared sensing device is set at the maximum limit water level in the water bath 1. When the water in the water bath 1 no longer overflows the low water level infrared sensing device, the low water level infrared sensing device sends a signal to the controller to open the subsequent water replenishment system such as the solenoid valve 13 to replenish the water bath 1 with constant temperature water; during the water replenishment process, when the water level in the water bath 1 overflows the high water level infrared sensing device, the high water level infrared sensing device sends a signal to the controller to close the solenoid valve 13 and other water replenishment systems.

[0042] In this embodiment, the specified temperatures of the automatic thermostatic valve 8 and the temperature probe 11 are both 37 degrees. The automatic thermostatic valve 8 controls the temperature of the water flowing out of the automatic thermostatic valve 8 at 37 degrees. The temperature probe 11 detects whether the water temperature in the confluence pipe 5 is maintained at 37 degrees. If so, the automatic return valve opens the first input end and closes the second input end, and the constant temperature water flows into the output pipe 7; if not, the automatic thermostatic valve 8 opens the second input end and closes the first input end, and the water that does not meet the water temperature standard flows into the return pipe 6, and then returns to the hot water pipe 4 and flows into the automatic thermostatic valve 8.

[0043] Furthermore, in an embodiment of the present invention, there is also an operation input port 22, which can be used to set various data after cooperating with the controller, such as the setting of the temperature in the water bath 1, the temperature setting selected by the two output ends of the temperature probe 11 connected to the automatic reflux three-way valve 10, and the setting for determining the minimum limit water level height and the maximum limit water level height of the switch solenoid valve 13.

[0044] Furthermore, in an embodiment of the present invention, the solenoid valve 13 can also be a solenoid valve 13 with a flow control function. When the solenoid valve 13 is opened to replenish water, the speed of the water flow is controlled. Although the replenishing water temperature is set to 37 degrees and the temperature in the water bath 1 is also 37 degrees, there is still a certain temperature difference between the two. The stem cell culture environment has very strict temperature requirements. If a large amount of replenishing water enters at once, the water temperature in the water bath 1 will definitely change greatly. Of course, this large water temperature change is relative to the stem cell culture experiment. Therefore, the solenoid valve 13 with a flow control function has unique advantages for the embodiment of the present invention in the stem cell culture experiment.

[0045] Furthermore, in an embodiment of the present invention, the heating section 9 on the hot water pipe 4 is configured as a spiral pipe, and a heating plate is attached to the outside of the spiral pipe. The configuration of the spiral pipe can allow the heating pipe to have as long a heating section 9 as possible in a limited laboratory, and the heating plate attached to the outside of the spiral pipe can heat the water in the spiral pipe faster, with higher heating efficiency. In other specific embodiments, the heating portion may be a heating device such as a heating wire placed in the heating section 9.

[0046] Further, in the embodiment of the present invention, the automatic thermostatic valve 8 is provided with a temperature adjustment knob 16, a display screen 15 for setting the output temperature, and a flow adjustment knob 17. A thermal element is installed at the output end of the automatic thermostatic valve 8, with a special wax / copper mixture as the temperature sensing medium, installed at the mixing place of hot water and cold water in the automatic thermostatic valve 8 to sense the mixed water temperature, and according to the temperature setting value on the valve, the mixing ratio of cold and hot water is automatically adjusted by driving the valve stem. When the temperature adjustment knob 16 is set to a certain temperature, no matter how the cold and hot water inlet temperature and pressure change, the ratio of cold and hot water entering the water outlet also changes accordingly, so that the water temperature flowing out of the output end is always kept constant, that is, the water temperature flowing into the confluence pipe 5 is controlled. The above-mentioned internal setting of the automatic thermostatic valve 8 is equivalent to a temperature control device. In other specific embodiments, the temperature control device can also be other devices that can ensure that the water temperature at the output end of the automatic thermostatic valve 8 is the set value. This embodiment is used for stem cell culture experiments, and the output water temperature of the automatic thermostatic valve 8 can be set to 37 degrees. A liquid flow meter is provided at the output end of the automatic thermostatic valve 8 to control the water flow rate out of the automatic thermostatic valve 8, and further control the water flow rate in the entire water replenishment system. In other specific embodiments, the flow controller can also be other devices that can achieve the water flow rate at the output end of the automatic thermostatic valve 8. The specific flow value of the flow controller is set by the flow adjustment knob 17. The shapes of the temperature adjustment knob 16 and the flow adjustment knob 17 are set to conform to the shape of human hand grip.

[0047] Furthermore, in the embodiment of the present invention, a one-way water stop valve 14 can be provided in the return pipe 6 to prevent the water supply system from backflowing in the return pipe 6. A one-way water stop valve 14 can also be provided in each branch pipe 2 to prevent the water in the water bath 1 from backflowing into the water supply system.

[0048] Further, in the embodiment of the present invention, the output end of the cold water pipe 3 and the output end of the hot water pipe 4 can be provided with a filter screen to filter impurities, scale, etc. in the external water flow to prevent them from entering the water bath 1 and contaminating the culture environment of stem cells. The output end of the cold water pipe 3 and the output end of the hot water pipe 4 can be removed from the two input ends of the automatic thermostatic valve 8, and then the filter screen therein can be taken out, thereby realizing the removal and replacement of the filter screen. In other specific embodiments, the filter screen can also be installed at the input end of the cold water pipe 3 and the input end of the hot water pipe 4. When the input end of the cold water pipe 3 and the input end of the hot water pipe 4 are connected to the external water pipe, they can be easily disassembled, which can facilitate the replacement of the filter screen.

[0049] See also Figure 2 Further, in the embodiment of the present invention, a plurality of water inlets 18, such as four or six, may be provided on the water bath 1, and these water inlets 18 are evenly distributed around the water bath 1. All water inlets 18 are provided with a partition 19, and a plurality of through holes 20 are opened on the partition 19. The provision of a plurality of through holes 20 allows the constant temperature water added to the water bath 1 to be output in the form of a plurality of water flows, so that the constant temperature water can be mixed with the water in the water bath 1 more quickly. The plurality of water inlets 18 and the provision of the perforated partition 19 at the water inlet 18 are both for the purpose of allowing the supplemented water to mix with the water in the water bath 1 more quickly.

[0050] Furthermore, in the embodiment of the present invention, the height of the total water inlet 12 is higher than the height of the maximum water level limit, which is helpful to prevent the water in the water bath 1 from flowing back into the water replenishment system.

[0051] In the entire water replenishment system, when the water bath 1 does not need water replenishment, the pipe between the solenoid valve 13 and the automatic reflux three-way valve 10 becomes a closed space. When the water bath 1 does not need water replenishment, the water temperature in this section of the pipe will drop. When water replenishment is needed next time, the water in this section of the pipe may not reach the specified temperature. To address this problem, a drain valve can be installed on this section of the pipe. Before each water replenishment, the controller sends a signal to the drain valve to make the drain valve work and drain this section of water.

[0052] Furthermore, the embodiment of the present invention also includes a temperature detection device, an alarm indicator light 26 and a communication module. The temperature detection device is arranged in the water bath 1, and the setting height is lower than the minimum limit water level height of the water bath 1. The temperature detection device is used to detect the water temperature in the water bath 1. The temperature detection device, the alarm indicator light 26 and the communication module are respectively connected to the controller electrical signal. If the water bath 1 has a system failure or other abnormal conditions, the alarm indicator light 26 and the communication module will be triggered. The alarm indicator light 26 is used to remind the personnel in the laboratory that there is a problem with the water bath 1, and the communication module is used to send information to relevant personnel. System failure or abnormal conditions, for example: the water bath 1 is provided with a safe water level range and a safe water temperature range. When the water level detection device detects that the water level in the water bath 1 is not within the safe water level range, the alarm indicator 26 and the communication module are triggered to issue an alarm prompt and notify relevant personnel; when the temperature detection device detects that the water temperature in the water bath 1 is not within the safe water temperature range, the alarm indicator 26 and the communication module are triggered to issue an alarm prompt and notify relevant personnel; the water bath 1 is provided with an emergency power supply. Once the conventional power supply is cut off, the emergency power supply immediately starts and triggers the alarm indicator 26 and the communication module to issue an alarm prompt and notify relevant personnel, etc. The operation input port 22 can be used to set the safe water level range and the safe water temperature range. The setting of the alarm indicator 26, the communication module and the corresponding supporting facilities is conducive to timely discovery and rapid resolution of problems after problems occur in the water bath 1.

[0053] See also Figure 3 Furthermore, the embodiment of the present invention also includes an operation display platform 21, on which the operation input port 22 and the alarm indicator light 26 are both located. A water level display 23, an actual temperature display 24, and a set temperature display 25 can also be provided on the operation display platform 21. The water level detection device detects the actual water level height in the water bath 1 and sends a signal to the controller, and the controller controls the water level display 23 to display the actual water level value. If the water level detection device previously used a device such as an infrared sensing device that cannot detect a specific water level, then if a water level display 23 is required, a water level detection device that can detect a specific water level needs to be installed in the water bath 1.

[0054] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, if these changes fall within the scope of the claims of the present invention and their equivalents, they still fall within the protection scope of the present invention.

Claims

1. An automatic constant temperature water replenishing device for stem cell culture, characterized in that: It includes a water bath, a water supply system, a water level detection device and a controller; At least one water inlet and a corresponding diversion pipe are provided on the periphery of the water bath, and the setting height of the water inlet is lower than the minimum limit water level height of the water bath, and each of the water inlets is connected to the output end of the corresponding diversion pipe; the water level detection device is arranged in the water bath; The water replenishment system includes a cold water pipeline, a hot water pipeline, a confluence pipeline, a return pipeline and an output pipeline, and also includes: An automatic thermostatic valve, wherein a first input end of the automatic thermostatic valve is communicated with an output end of the cold water pipe, a second input end of the automatic thermostatic valve is communicated with an output end of the hot water pipe, and an output end of the automatic thermostatic valve is communicated with an input end of the converging pipe; an input end of the cold water pipe and an input end of the hot water pipe are both communicated with an external water pipe, a heating section is provided on the hot water pipe, and a heating portion is provided on the heating section; An automatic reflux three-way valve, wherein the input end of the automatic reflux three-way valve is connected to the output end of the merging pipe, and the first output end of the automatic reflux three-way valve is connected to the input end of the output pipe; the second output end of the automatic reflux three-way valve is connected to the input end of the reflux pipe, and the output end of the reflux pipe is connected to the hot water pipe; a temperature probe is provided on the output end of the merging pipe; The output end of the output pipeline is the main water inlet, and the input ends of all the branch pipelines are connected to the main water inlet; a solenoid valve is provided on the output pipeline near the automatic reflux three-way valve; The water level detection device, the heating unit, the temperature probe, the automatic reflux three-way valve and the solenoid valve are respectively connected to the controller with electrical signals; When the water level detection device detects that the water level in the water bath is lower than the set minimum water level, the controller controls the solenoid valve to open; after the water level detection device detects that the water level in the water bath is higher than the set maximum water level, the controller controls the solenoid valve to close; When the temperature probe detects that the temperature of the confluent pipe meets the set temperature, the controller controls the first output end of the automatic reflux three-way valve to open and the second output end to close; when the temperature probe detects that the temperature of the confluent pipe deviates from the set temperature, the controller controls the first output end of the automatic reflux three-way valve to close and the second output end to open; The heating section is a spiral pipe, and the heating part is attached to the spiral pipe; Each of the diversion pipelines is provided with a one-way water stop valve.

2. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: The automatic thermostatic valve is provided with a temperature controller and a flow controller. The temperature controller is used to set and control the temperature of the water flow output from the output end of the automatic thermostatic valve, and the flow controller is used to control the size of the water flow output from the output end of the automatic thermostatic valve.

3. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: A one-way water stop valve is arranged on the return pipe.

4. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: The output end of the cold water pipe is provided with a first filter screen, and the output end of the hot water pipe is provided with a second filter screen.

5. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: Each of the water inlets is provided with a partition plate, and a plurality of through holes are opened on the partition plate.

6. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: The number of the water inlets is four or six, and the water inlets are evenly distributed around the water bath.

7. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: The total water inlet is arranged at a height higher than the maximum limit water level of the water bath.

8. The automatic constant temperature water replenishing device for stem cell culture according to claim 1, characterized in that: It also includes a temperature detection device, an alarm indicator light and a communication module; the temperature detection device is arranged in the water bath, and the setting height of the temperature detection device is lower than the minimum limit water level height of the water bath; the temperature detection device, the alarm indicator light and the communication module are respectively connected to the controller with electrical signals.

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