Microfluidic Chip

Abstract

The present invention discloses a microfluidic chip, comprising: a chip body, having an injection site, an output site and a driving force entry site; the injection site, the output site and the driving force entry site are arranged on the same side of the chip body, and the injection site and the output site are arranged at intervals; a microfluidic channel, arranged inside the chip body; the microfluidic channel includes an injection flow channel, an output flow channel and a main flow channel, the injection flow channel and the output flow channel are respectively connected to the main flow channel, and along the same direction of the main flow channel, the injection flow channel and the output flow channel are alternately arranged; a positioning component. The technical solution of the present invention solves the technical problems of the complex structure and difficult manufacturing of traditional microfluidic chips and the inaccurate quantification of sample volume by optimizing the structure of the microfluidic chip, and realizes the batch automatic operation of the microfluidic chip.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a microfluidic chip. Background Art

[0002] Microfluidic chip, through precise manipulation and control of microfluids in microchannel networks, realizes the functions of the entire chemical and biological laboratory, and is also called "Lab on a chip". Microfluidic chip technology (Microfluidics) integrates the basic operating units of sample preparation, reaction, separation, detection, etc. in biological, chemical, and medical analysis processes into a micrometer-scale chip to automatically complete the entire analysis process. Due to its huge potential in the fields of biology, chemistry, and medicine, it has developed into a new research field that alternates between disciplines such as biology, chemistry, medicine, fluids, electronics, materials, and mechanics. Micro total analysis system (μTAS) refers to an in vitro analysis system that integrates sample processing, manipulation, reaction, separation, and detection, and has the characteristics of trace, high efficiency, rapidity, high throughput, miniaturization, integration, and automation. At the same time, there are significant scale effects in the micro total analysis system, such as laminar flow effect, rapid mass transfer and heat transfer effect, etc., which make it show unique advantages different from macroscopic systems.

[0003] In Vitro Diagnosis (IVD) refers to the testing of human samples (blood, body fluids, tissues, etc.) outside the human body to obtain clinical diagnostic information. It is a product and service for judging diseases and collective functions, generally including diagnostic equipment (instruments) and diagnostic reagents. As an effective biochemical reaction carrier, microfluidic chips are of great significance for in vitro diagnosis, especially point-of-care testing (POCT). At present, the problems of sample loading and sample volume quantification with microfluidic chips are the main problems affecting the realization of automation, micro-quantification and quantitative detection of microfluidic chips.

[0004] In the related art, the sampling and injection methods for microfluidic chips mainly include the following: solid-liquid two-phase chip, which is to encapsulate part of the reaction sample inside the microfluidic chip chamber. Although an additional antibody fixation step is added, the reaction process is only carried out on the solid surface, which is inefficient; liquid chip storage bag, which is to encapsulate part of the reaction sample in the chip storage bag. During the reaction, the reaction sample is pressed out by external force or punctured by a puncturing device and released by a centrifugal device, but the liquid chip storage bag is prone to liquid residue, and it is difficult to achieve a few microliters of trace reaction sample storage; liquid chip injection pump continuous sampling and injection, it should be understood that continuous sampling is not conducive to batch automation of the chip and the amount of reaction sample is large, and the liquid circuit control is complex when multiple samples are sampled; liquid chip sample needle loading, its main principle is to combine positive pressure and / or negative pressure injection, but the structure of the chip using this method is relatively complex, and an additional sample needle cleaning step needs to be added. Therefore, the design of a microfluidic chip with low consumption of required reaction samples, accurate quantification of sample volume and simple structure is imminent. Summary of the invention

[0005] The main purpose of the present invention is to provide a microfluidic chip, aiming to solve the technical problems of the traditional microfluidic chip having a complex structure and inaccurate sample volume quantification.

[0006] To achieve the above object, the present invention provides a microfluidic chip, comprising:

[0007] The chip body has a sample injection site, a sample output site and a driving force input site; the sample injection site, the sample output site and the driving force input site are arranged on the same side of the chip body, and the sample injection site and the sample output site are arranged at intervals;

[0008] A microfluidic channel is provided inside the chip body; the microfluidic channel includes an inlet channel, an outlet channel and a main channel, the inlet channel and the outlet channel are respectively connected to the main channel, and the inlet channel and the outlet channel are alternately arranged; there are multiple outlet channels, and the volume of the main channel between two adjacent outlet channels is the sample quantitative volume;

[0009] The injection site is communicated with the injection flow channel, and the output site is communicated with the output flow channel; the driving force entry site is communicated with the microfluidic channel, and the driving force entry site is used to connect a driving device to drive the movement of the fluid in the microfluidic channel;

[0010] The positioning component is arranged on the chip body and is used to limit the position of the microfluidic chip.

[0011] Optionally, the sample inlet channel and the sample outlet channel are respectively arranged at an angle to the main channel.

[0012] Optionally, the width of the main channel is 0.01-1 mm.

[0013] Optionally, the cross-sectional dimension of the main flow channel is larger than the cross-sectional dimension of the inlet flow channel or the cross-sectional dimension of the outlet flow channel; and / or,

[0014] The cross-sectional shape of the main channel is rectangular or trapezoidal.

[0015] Optionally, the microfluidic chip further comprises a plurality of functional chambers, wherein the plurality of multifunctional chambers are arranged on the sample inlet channel or the sample outlet channel, and are used to provide accommodation space for reaction samples entering the microfluidic channel or waste liquid generated after the reaction is completed.

[0016] Optionally, the inlet flow channel comprises a mixing flow channel, and the mixing flow channel comprises a main mixing flow channel and at least two branch mixing flow channels;

[0017] The main mixing channel is connected to the main channel, and each of the branch mixing channels corresponds to one of the injection sites and one of the multifunctional chambers.

[0018] Optionally, the size of the microfluidic chip is 0.5 to 200 mm; and / or,

[0019] The volume range of the multifunctional chamber is 0.009-1000mm 3 .

[0020] Optionally, the microfluidic chip further has a draft structure; and / or,

[0021] The material of the microfluidic chip is one of glass, quartz, polydimethylsiloxane, polymethyl methacrylate, polycarbonate and polystyrene or their derivatives; and / or,

[0022] The driving mode of the microfluidic chip is one of air pressure driving, voltage driving and magnetic field driving.

[0023] Optionally, the positioning component includes a first positioning structure, which is arranged at the edge of the chip body; the first positioning structure is used to position the microfluidic chip during the removal process; and / or,

[0024] The positioning assembly includes a second positioning member, which is convexly arranged on the peripheral side of the chip body to position the microfluidic chip during transportation after the microfluidic chip is taken out; and / or, the positioning assembly includes a third positioning member, which is convexly arranged on the chip body to position the microfluidic chip before entering the reaction process; and / or,

[0025] The positioning component includes a fourth positioning portion, and the fourth positioning portion is located in the microfluidic channel to position the microfluidic chip during the reaction process.

[0026] Optionally, the chip body comprises a protrusion layer, a flow channel layer and a thin film layer bonded in sequence;

[0027] The sample injection site, the sample output site and the driving force entry site are arranged on a side of the protrusion layer away from the flow channel layer;

[0028] The main flow channel is arranged on the flow channel layer; the injection flow part is arranged on the flow channel layer, and the other part is arranged on the protrusion layer, so as to connect the injection site with the main flow channel;

[0029] The injection channel is partially disposed on the channel layer and the other part is disposed on the protrusion layer to connect the injection site with the main flow channel;

[0030] The sample outlet channel is partially disposed on the channel layer and the other part is disposed on the protrusion layer to connect the sample outlet site with the main channel; or,

[0031] The chip body comprises a flow channel layer and a thin film layer bonded in sequence;

[0032] The sample injection point, the sample output point and the driving force entry point are all arranged on a side of the flow channel layer away from the film layer;

[0033] The main channel, the sample inlet channel and the sample outlet channel are all arranged in the channel layer; the sample inlet channel connects the sample inlet site and the main channel, and the sample outlet channel connects the sample outlet site and the main channel.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] In the technical solution of the present invention, by designing the microfluidic channel structure in the microfluidic chip, the volume of the sample injection is accurately quantified. Specifically, the injection site, the injection flow channel, the main flow channel, the sample outlet flow channel and the sample outlet site are set to realize the closed-loop flow of the sample injection; at the same time, the driving force entry site is set to automatically drive the inflow, mixing, reaction, outflow, etc. of the sample injection sample entering the microfluidic channel; and, by alternately setting the injection circulation and the sample outlet flow channel, it is ensured that the mixing and complete reaction of different sample injection samples on the main flow channel, and the waste liquid after the reaction is completed is brought into the sample outlet flow channel, and the volume of the sample injection sample (i.e., the volume of the main flow channel of the two adjacent sample outlet flow channels) is accurately quantified, while realizing the accurate quantification of the sample volume, the consumption of the sample required for injection is greatly reduced. By setting the positioning component, the automated batch operation of the microfluidic chip is realized, which greatly reduces the labor cost. And due to the setting of the positioning component, the position accuracy of the microfluidic chip in the automation process is greatly enhanced, providing a stable and accurate environment for the reaction and post-reaction measurement of subsequent samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0037] Figure 1 An exploded view of a microfluidic chip in one embodiment of the present invention;

[0038] Figure 2 A flow channel distribution diagram of a flow channel layer of a microfluidic chip in one embodiment of the present invention;

[0039] Figure 3 is a schematic diagram of fluid flow in a microfluidic channel in one embodiment of the present invention;

[0040] Figure 4 An exploded view of a microfluidic chip according to another embodiment of the present invention.

[0041] Description of Figure Numbers:

[0042] Label name Label name 100 Chip body 220 Injection channel 200 Microfluidic channels 230 Sample flow channel 300 Positioning components 240 Mixing channel 400 Functional chamber 241 Main mixing channel 500 Draft structure 242 Mixing runner 110 Raised layer 310 The first positioning structure 120 Runner layer 320 Second positioning member 130 Thin film layer 330 The third positioning piece 210 Main channel 340 Fourth positioning part

[0043] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0046] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0047] See also Figures 1 to 4 The present invention provides a microfluidic chip, comprising:

[0048] The chip body 100 has a sample injection site, a sample output site and a driving force input site; the sample injection site, the sample output site and the driving force input site are arranged on the same side of the chip body 100, and the sample injection site and the sample output site are arranged at intervals;

[0049] The microfluidic channel 200 is arranged inside the chip body 100; the microfluidic channel 200 includes an inlet channel 220, an outlet channel 230 and a main channel 210, the inlet channel 220 and the outlet channel 230 are respectively connected to the main channel 210, and the inlet channel 220 and the outlet channel 230 are alternately arranged; there are multiple outlet channels 230, and the volume of the main channel 210 between two adjacent outlet channels 230 is the sample quantitative volume;

[0050] The injection site is connected to the injection channel 220, and the outlet site is connected to the outlet channel 230; the driving force entry site is connected to the microfluidic channel 200, and the driving force entry site is used to connect a driving device to drive the movement of the fluid in the microfluidic channel 200;

[0051] The positioning component 300 is disposed on the chip body 100 and is used to limit the position of the microfluidic chip.

[0052] In this embodiment, in order to realize the detection of different injected samples, an injection site is set. In this way, different samples can be added to the microfluidic chip according to actual detection needs, which greatly improves the universality of the microfluidic chip. In order to realize the concentration, separation and detection of the samples after the reaction, a sample outlet site is set to realize the collection of the samples after the reaction. In order to realize the automated injection, automated reaction, detection, etc. of the microfluidic chip, a driving force entry site is set to allow external force to enter the microfluidic channel 200, thereby promoting the movement of the fluid in the microfluidic channel 200 and realizing the control of the sample reaction process.

[0053] Furthermore, in order to achieve accurate quantification of the volume of the injected sample, an alternately arranged injection channel 220 and an outlet channel 230, as well as a main channel 210 are provided. Specifically, the injection site, the injection channel 220, the main channel 210, the outlet channel 230 and the outlet site realize the closed-loop flow of the sample; at the same time, under the action of the driving force, the automatic inflow, mixing, reaction, outflow and other operations of the sample are realized, which greatly improves the efficiency of sample detection; and, through the alternately designed injection channel 220 and the outlet channel 230, it is ensured that different injected samples are mixed and completely reacted on the main channel 210, and the waste liquid after the reaction is completed is brought into the outlet channel 230, and the volume of the injected sample (i.e., the volume of the main channel 210 of the two adjacent outlet channels 230) is accurately quantified, while achieving accurate quantification of the sample volume, the consumption of the sample required for injection is greatly reduced.

[0054] Furthermore, the positioning component 300 is provided to realize the automated batch operation of the microfluidic chip, which greatly reduces the labor cost. In addition, due to the provision of the positioning component 300, the position accuracy of the microfluidic chip in the automated process is greatly enhanced, providing a stable and accurate environment for the subsequent sample reaction and post-reaction measurement.

[0055] For example, but not limited to, in one embodiment, the sample injection site is the driving force entry site, and a positive pressure driving device may be used to drive the sample. In another embodiment, the sample output site is the driving force entry site, and a negative pressure driving device may be used to drive the sample. It should be understood that in other embodiments, in order to reduce the probability of bubbles generated by the sample in the microfluidic channel 200, the driving force entry site may be set separately.

[0056] Optionally, the sample inlet channel 220 and the sample outlet channel 230 are respectively arranged at an angle to the main channel 210 .

[0057] In this embodiment, in order to integrate more volume of sample injection for convenient detection and to maximize the use of the microfluidic chip, the injection channel 220 / the outlet channel 230 is set at an angle to the main channel 210, that is, the plane formed by the injection channel 220 / the outlet channel 230 and the main channel 210 is not on the same plane. In this way, the injection site / the outlet site can be set on the front of the chip body, while increasing the additional points of the injection site / the outlet site, and increasing the injection measurement of different samples. The applicability of the microfluidic chip is greatly improved.

[0058] For example but not limited to, the sample inlet channel 220 is disposed perpendicular to the main channel 210 , and / or the sample outlet channel 230 is disposed perpendicular to the main channel 210 .

[0059] It should be understood that, in other embodiments, the sample inlet channel 220 and / or the sample outlet channel 230 may be disposed on the same horizontal plane as the main channel 210 .

[0060] Optionally, the width of the main channel 210 is 0.01-1 mm.

[0061] In this embodiment, the width of the main channel 210 is controlled between 0.01 and 1 mm, taking into account the cost of machining accuracy, the ability of the sample to complete a complete reaction after entering the microfluidic channel 200, and the driving device driving the fluid in the microfluidic channel 200. Preferably, the width of the main channel 210 is in the range of 0.03 to 0.5 mm.

[0062] Optionally, the cross-sectional dimension of the main flow channel 210 is greater than the cross-sectional dimension of the sample inlet flow channel 220 or the cross-sectional dimension of the sample outlet flow channel 230 .

[0063] In this embodiment, in order to achieve a sufficient reaction of the injected sample in the main channel 210 , the size of the main channel 210 is designed to be larger than the size of the injection channel 220 or the size of the outlet channel 230 .

[0064] Optionally, the cross-sectional shape of the main channel 210 is rectangular or trapezoidal.

[0065] In this embodiment, for the convenience of processing, the main flow channel 210 is set as a cubic flow channel. For example but not limited to, the main flow channel 210 is a cubic flow channel or the main flow channel 210 is a trapezoidal flow channel. In other embodiments, the main flow channel 210 can also be a cylindrical flow channel.

[0066] Optionally, the microfluidic chip also includes a plurality of functional chambers 400, and the plurality of multifunctional chambers 400 are arranged on the sample inlet channel 220 or the sample outlet channel 230 to provide accommodation space for reaction samples entering the microfluidic channel 200 or waste liquid generated after the reaction is completed.

[0067] In this embodiment, a functional chamber 400 is provided to provide a temporary storage space for the sample. The functional chamber 400 has at least the following functions: first, storage of the sample; second, providing a mixing space for different sample injections; third, storage of the sample (waste liquid) after the reaction. The number of the multifunctional chambers 400 is greater than or equal to the sum of the number of the sample injection channel 220 and the sample outlet channel 230. That is, at least one functional chamber 400 is provided on each of the sample injection channels 220, and at least one functional chamber 400 is provided on each of the sample outlet channels 230. For example, but not limited to, one functional chamber 400 is provided on each of the sample injection channels 220, and one functional chamber 400 is provided on each of the sample outlet channels 230.

[0068] Optionally, the inlet flow channel 220 includes a mixing flow channel 240, and the mixing flow channel 240 includes a main mixing flow channel 241 and at least two branch mixing flow channels 242;

[0069] The material mixing main channel 241 is connected to the main channel 210 , and each of the material mixing branch channels 242 corresponds to one of the injection sites and one of the multifunctional chambers 400 .

[0070] In this embodiment, in order to improve the adaptability of the microfluidic chip to the injection samples of different requirements, a mixing channel 240 is provided. In the mixing channel 240, two or more different injection samples can be fully mixed. For example, but not limited to, the mixing channel 240 includes a mixing main channel 241, and two mixing branch channels 242, and correspondingly, at least one functional chamber 400 is respectively provided on the two mixing branch channels 242, and an injection site is respectively provided on the two mixing branch channels 242. In this way, under the action of the sampling needle, different injection samples can enter the mixing branch channel 242 of the microfluidic chip through different injection sites, and then enter the functional chamber 400, and enter the mixing main channel 210 through the mixing branch channel for full mixing, and then the mixed mixture is passed into the main channel 210 to react with other injection samples, etc., which greatly improves the adaptability of the microfluidic chip to injection samples of different requirements.

[0071] Optionally, the size of the microfluidic chip is 0.5 to 200 mm.

[0072] In this embodiment, in order to save raw materials for preparing chips to the maximum extent and reduce raw materials for industrial production, after a large number of experimental tests, the appearance size of the microfluidic chip is controlled within 0.5 to 200 mm. Preferably, the appearance size of the microfluidic chip is 0.5 to 100 mm.

[0073] Optionally, the volume range of the multifunctional chamber 400 is 0.009-1000mm 3 .

[0074] In this embodiment, in order to simultaneously realize the storage of the injected sample and the volume limitation of the microfluidic chip, the volume of the multifunctional chamber 400 is controlled within a range of 0.009 to 1000 mm 3 Preferably, the volume range of the multifunctional chamber 400 is 0.125-125 mm 3 .

[0075] Optionally, the microfluidic chip further has a draft structure 500 .

[0076] In this embodiment, in order to facilitate processing and improve industrial production efficiency, a demolding structure 500 is further provided on the chip body 100. For example but not limited to, the demolding structure 500 is provided at the third positioning member 330 and the multifunctional chamber 400.

[0077] Optionally, the material of the microfluidic chip is one of glass, quartz, polydimethylsiloxane, polymethyl methacrylate, polycarbonate and polystyrene, or a derivative thereof.

[0078] In this embodiment, in order to save costs and improve the visibility and durability of the microfluidic chip, polymethyl methacrylate is used as the processing material of the microfluidic chip.

[0079] Optionally, the microfluidic chip is driven by one or more of air pressure drive, voltage drive and magnetic field drive.

[0080] Optionally, the positioning component 300 includes a first positioning structure 310 , and the first positioning structure 310 is disposed at an edge of the chip body 100 ; the first positioning structure 310 is used to position the microfluidic chip during the removal process.

[0081] In this embodiment, in order to achieve accurate positioning of the chip body 100 during the removal process, a first positioning structure 310 is provided. The first positioning structure 310 is a sideline shape structure, which is provided on the periphery of the chip body 100. It should be understood that the specific sideline shape structure of the first positioning structure 310 matches the sideline shape structure on the removal device, so that when the removal device takes out the microfluidic chip from the chip box, the chip body 100 can be accurately taken out, and the position of the chip body 100 after removal can be accurately positioned. For example, but not limited to, the first positioning structure 310 is a toothed structure provided on the chip body 100. In order to enhance the accuracy of the position of the microfluidic chip during the removal process, the toothed structure is provided in plurality, and the plurality of toothed structures are symmetrically arranged. In other embodiments, in order to facilitate processing, a toothed structure may also be provided.

[0082] Optionally, the positioning assembly 300 includes a second positioning member 320, which is protruding from the peripheral side of the chip body 100 to position the microfluidic chip during transportation after the microfluidic chip is taken out.

[0083] In this embodiment, in order to achieve accurate positioning of the chip body 100 during transportation, a second positioning member 320 is provided. It should be understood that the second positioning member 320 matches the limiting structure on the transport device, so that after the removal device takes out the microfluidic chip, the limiting structure on the transport device cooperates with the second positioning member 320 to achieve accurate positioning of the position of the microfluidic chip during transportation. For example, but not limited to, the second positioning member 320 is a protrusion. In order to enhance the accuracy of the position of the microfluidic chip during transportation, the protrusion is provided in plurality, and the plurality of protrusions are arranged in a force-symmetrical manner. In other embodiments, in order to facilitate processing and reduce processing costs, one protrusion may be provided.

[0084] Optionally, the positioning assembly 300 includes a third positioning member 330 , and the third positioning member 330 is protruded from the chip body 100 to position the microfluidic chip before entering the reaction process.

[0085] In this embodiment, in order to achieve precise control of the position of the chip body 100 before entering the reaction, a third positioning member 330 is provided. It should be understood that the third positioning member 330 matches the positioning module on the pre-reaction positioning device, so that when the transport device transports the microfluidic chip to the specified position, the pre-reaction positioning device achieves precise positioning of the microfluidic chip through the cooperation between the positioning module and the third positioning member 330. For example, but not limited to, the third positioning member 330 is a positioning column convexly arranged on the chip body 100. The positioning column is convexly arranged on the front of the chip body 100, and when the transport device transports the chip body 100 to the specified position, the positioning module on the reaction positioning device cooperates with the third positioning member 330 to achieve the limitation of the position of the microfluidic chip to be reacted. Generally, in order to strengthen the precise positioning of the position of the microfluidic chip before the reaction, a plurality of positioning columns are provided, and the plurality of positioning columns are arranged in a force-symmetrical manner. In other embodiments, in order to reduce costs, a positioning column can be provided.

[0086] In one embodiment, the third positioning member 330 is a hollow positioning column, the hollow positioning column is connected to the microfluidic channel 200, and the driving force entry point is set at the hollow part of the hollow positioning column. At this time, the external driving device is connected to the microfluidic chip through the hollow positioning column. It should be understood that the external driving device is provided with a groove for the hollow positioning column to be inserted.

[0087] In another embodiment, the periphery of the hollow positioning column is also provided with a step, so as to achieve the sealing between the external driving device and the microfluidic chip when the external driving device is connected to the microfluidic chip, thereby preventing the external driving device from generating small bubbles when pushing the fluid in the microfluidic channel 200 to move, thereby reducing the accuracy of the sample reaction. It should be understood that in other embodiments, the seal between the external driving device and the microfluidic chip is a static seal. For example, but not limited to, the sealing method is a sealing gasket seal, an elastic pressure seal, a spring seal, etc.

[0088] Optionally, the positioning assembly 300 includes a fourth positioning portion 340 , and the fourth positioning portion 340 is located in the microfluidic channel 200 to position the microfluidic chip during the reaction process.

[0089] In this embodiment, in order to further realize the precise positioning of the microfluidic channel 200 to control the reaction process of the sample in the microfluidic chip, a fourth positioning portion 340 is also provided. Specifically, the fourth positioning portion 340 is a microfluidic channel 200 portion, that is, a fluorescent substance is provided in the microfluidic channel 200 portion. It should be understood that the fluorescent substance is provided with an optical detection device to match it. After the microfluidic chip is positioned by the pre-reaction positioning device, the fluorescent substance provided in the microfluidic channel 200 is detected by the optical detection device to further realize the precise positioning of the position of the microfluidic chip. For example, but not limited to, in order to reduce costs, the fourth positioning portion 340 is a mixing channel 240 portion, that is, the precise positioning of the entire microfluidic channel 200 is achieved only by the mixing channel 240 portion with a more special structure. That is, the fluorescent material is only disposed in the mixing channel 240 , and then the fluorescent material disposed in the mixing channel 240 is detected by the optical detection device, thereby achieving accurate positioning of the microfluidic chip.

[0090] Optionally, the chip body includes a protrusion layer 110, a flow channel layer 120 and a film layer 130 bonded in sequence;

[0091] The sample injection site, the sample output site and the driving force entry site are arranged on a side of the protrusion layer 110 away from the flow channel layer 120;

[0092] The main flow channel 210 is disposed on the flow channel layer 120; the injection flow portion is disposed on the flow channel layer 120, and the other portion is disposed on the protrusion layer 110, so as to connect the injection site with the main flow channel 210;

[0093] The injection channel 220 is partially disposed on the channel layer 120 and the other part is disposed on the protrusion layer 110 to connect the injection site with the main channel 210;

[0094] The sample outlet channel 230 is partially disposed on the channel layer 120 , and the other part is disposed on the protrusion layer 110 to connect the sample outlet site with the main channel 210 .

[0095] In this embodiment, in order to facilitate the processing of the chip body 100, the chip body 100 is provided with a raised layer 110, a flow channel layer 120 and a thin film layer 130. The raised layer 110 is mainly used to connect with an external device to realize the loading of the sample; specifically, the raised layer 110 is provided with the injection site, the sample outlet site, the driving force entry site and the functional chamber 400. The flow channel layer 120 is mainly used for mixing and reacting the sample; specifically, the microfluidic channel 200 is provided in the flow channel layer 120. The thin film layer 130 is mainly used to protect the flow channel layer 120 to prevent the flow channel layer 120 from being damaged by external mechanical forces. For example, but not limited to, the thin film layer 130 is a smooth plate.

[0096] In other embodiments, in order to reduce the assembly cost of the chip body 100 , the protrusion layer 110 and the flow channel layer 120 are integrally formed.

[0097] Optionally, the chip body includes a flow channel layer 120 and a thin film layer 130 bonded in sequence;

[0098] The sample injection point, the sample output point and the driving force entry point are all arranged on a side of the flow channel layer 120 away from the film layer 130;

[0099] The main channel 210 , the sample inlet channel 220 and the sample outlet channel 230 are all arranged in the channel layer 120 ; the sample inlet channel 220 connects the sample inlet site and the main channel 210 , and the sample outlet channel 230 connects the sample outlet site and the main channel 210 .

[0100] In this embodiment, in order to simplify the structure of the microfluidic chip and reduce the number of bonding times, a two-layer chip structure is provided, namely, a flow channel layer 120 and a thin film layer 130. It should be understood that the flow channel layer 120 has a certain thickness so that the inlet flow channel 220 and / or the outlet flow channel 230 can be arranged at an angle with the main flow channel 210.

[0101] From the above, the working process of the microfluidic chip is as follows: the microfluidic chip is stored on the chip holder in the chip box. When the microfluidic chip is needed, the chip box is opened and the microfluidic chip is ejected; then the edge shape structure on the taking-out device is aligned with the first positioning structure 310 on the chip body 100 to take out the microfluidic chip; then, the second limiting member provided on the microfluidic chip is limited in position by the limiting structure on the transport device (that is, the protrusion provided on the periphery of the chip body 100), thereby realizing the positioning of the microfluidic chip during transportation; then, when the transport device transports the microfluidic chip to the specified position, the microfluidic chip is positioned before reaction. The positioning module provided on the device is aligned with the third positioning member 330 (i.e., the positioning column protruding from the chip body 100) on the chip body 100, thereby realizing the limitation of the position of the microfluidic chip before the sample reaction is transported to the designated position; finally, after the initial positioning of the position of the microfluidic chip before the sample enters the reaction, in order to increase the accuracy of the reaction of the sample in the microfluidic chip (to facilitate the monitoring of the amount of sample reaction and the control of the reaction process), the fluorescent substance provided in the microfluidic channel 200 is detected by an optical detection device, thereby realizing the precise positioning of the microfluidic channel 200. At this point, the reaction monitoring process of the sample can be carried out.

[0102] The above descriptions are only optional embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. A microfluidic chip, characterized in that: include: The chip body has a sample injection site, a sample output site and a driving force entry site; The sample injection site, the sample output site and the driving force entry site are arranged on the same side of the chip body, and the sample injection site and the sample output site are arranged at intervals; A microfluidic channel is provided inside the chip body; the microfluidic channel includes an inlet channel, an outlet channel and a main channel, the inlet channel and the outlet channel are respectively connected to the main channel, and the inlet channel and the outlet channel are alternately arranged; a plurality of inlet channels and outlet channels are provided, and the volume of the main channel between two adjacent outlet channels is the sample quantitative volume; The injection site is connected to the injection flow channel, and the sample outlet site is connected to the sample outlet flow channel; the driving force entry site is connected to the microfluidic channel, and the driving force entry site is used to connect a driving device to drive the movement of the fluid in the microfluidic channel; the sample injected into the injection flow channel is mixed and completely reacted in the main flow channel, and after the reaction is completed, the waste liquid after the reaction is brought into the sample outlet flow channel, and a closed-loop flow of the sample is achieved among the injection site, the injection flow channel, the main flow channel, the sample outlet flow channel and the sample outlet site; A positioning component, disposed on the chip body, for limiting the position of the microfluidic chip; Wherein, the sample inlet channel and the sample outlet channel are respectively arranged at an angle to the main channel.

2. The microfluidic chip according to claim 1, characterized in that: The width of the main channel is 0.01-1 mm.

3. The microfluidic chip according to claim 2, characterized in that: The cross-sectional dimension of the main flow channel is larger than the cross-sectional dimension of the inlet flow channel or the cross-sectional dimension of the outlet flow channel; and / or, The cross-sectional shape of the main channel is rectangular or trapezoidal.

4. The microfluidic chip according to any one of claims 1 to 3, characterized in that: The microfluidic chip also includes a plurality of functional chambers, which are arranged on the sample inlet channel or the sample outlet channel to provide accommodation space for reaction samples entering the microfluidic channel or waste liquid generated after the reaction is completed.

5. The microfluidic chip according to claim 4, characterized in that: The inlet flow channel includes a mixing flow channel, and the mixing flow channel includes a main mixing flow channel and at least two sub-mixing flow channels; The main mixing channel is connected to the main channel of the microfluidic channel, and each of the sub-mixing channels corresponds to one of the injection sites and one of the functional chambers.

6. The microfluidic chip according to claim 4, characterized in that: The size of the microfluidic chip is 0.5~200 mm; and / or, The volume range of the functional chamber is 0.009~1000 mm 3 .

7. The microfluidic chip according to any one of claims 1 to 3, characterized in that: The microfluidic chip further has a draft structure; and / or, The material of the microfluidic chip is one of glass, polydimethylsiloxane, polymethyl methacrylate, polycarbonate and polystyrene or their derivatives; and / or, The driving mode of the microfluidic chip is one of air pressure driving, voltage driving and magnetic field driving.

8. The microfluidic chip according to any one of claims 1 to 3, characterized in that: The positioning assembly includes a first positioning structure, which is arranged at the edge of the chip body; the first positioning structure is used to position the microfluidic chip during the removal process; The positioning assembly includes a second positioning member, which is convexly arranged on the peripheral side of the chip body to position the microfluidic chip during transportation after the microfluidic chip is taken out; The positioning assembly includes a third positioning member, and the third positioning member is protruding from the chip body to position the microfluidic chip before entering the reaction process; The positioning component includes a fourth positioning portion, and the fourth positioning portion is located in the microfluidic channel to position the microfluidic chip during the reaction process.

9. The microfluidic chip according to claim 8, characterized in that: The chip body comprises a protrusion layer, a flow channel layer and a thin film layer bonded in sequence; The sample injection site, the sample output site and the driving force entry site are arranged on a side of the protrusion layer away from the flow channel layer; The main flow channel is arranged in the flow channel layer; The injection channel is partially disposed on the channel layer, and the other part is disposed on the protrusion layer, so as to connect the injection site with the main flow channel; The sample outlet channel is partially arranged on the channel layer, and the other part is arranged on the protrusion layer, so as to connect the sample outlet site with the main channel.

10. The microfluidic chip according to claim 8, characterized in that: The chip body comprises a flow channel layer and a thin film layer bonded in sequence; The sample injection point, the sample output point and the driving force entry point are all arranged on a side of the flow channel layer away from the film layer; The main channel, the sample inlet channel and the sample outlet channel are all arranged in the channel layer; the sample inlet channel connects the sample inlet site and the main channel, and the sample outlet channel connects the sample outlet site and the main channel.

Images