DNA fragment, recombinant vector, transformant, and nitrogen fixation enzyme

Abstract

The DNA fragment encodes a nitrogen-fixing enzyme and has a base sequence of SEQ ID NO:1 or a base sequence with a 50% or greater identity to the base sequence of SEQ ID NO:1. The recombinant vector contains the DNA fragment. The transformant is transformed by the recombinant vector. The nitrogen-fixing enzyme is expressed by the transformant. The nitrogen-fixing enzyme includes any one or more of the amino sequences of SEQ ID NOs:34-65.

Description

Technical Field

[0001] The present invention relates to a DNA fragment encoding a nitrogen fixation enzyme, a recombinant vector containing the DNA fragment, a transformant transformed by the recombinant vector, and a nitrogen fixation enzyme. Background Art

[0002] Generally, the growth of Escherichia coli is inhibited in a culture medium without any nitrogen source (ammonium chloride, sodium nitrate, etc.), so for its proliferation, a nitrogen source must be added to the culture medium.

[0003] As a method of nitrogen fixation other than the conventional Haber–Bosch process, there is an enzymatic method using nitrogenase. In this method, nitrogen in the atmosphere can be fixed as ammonia in an environment of normal temperature and pressure. Therefore, in the presence of nitrogenase, the need for the addition of the above-mentioned nitrogen source can be eliminated.

[0004] On the other hand, the above-mentioned nitrogenase is a metalloenzyme containing V or Mo (see, for example, Patent Document 1).

[0005] Prior art literature

[0006] Patent Literature

[0007] Patent Document 1: JP 2016 / 136972 A (Paragraphs 0006 and 0007) Summary of the invention

[0008] Problem that the invention aims to solve

[0009] However, if nitrogen fixation is performed using Escherichia coli into which nitrogenase has been introduced, metal elements such as V or Mo that serve as active centers are insufficient, and therefore, metal elements such as V or Mo must be added so that the Escherichia coli into which nitrogenase has been introduced can proliferate in a culture medium that does not contain any nitrogen source.

[0010] An object of the present invention is to provide a DNA fragment encoding a nitrogen fixation enzyme that allows the elimination of the need to add a required nitrogen source to a culture medium for the proliferation of Escherichia coli, a recombinant vector containing the DNA fragment, a transformant transformed by the recombinant vector, and a nitrogen fixation enzyme.

[0011] Another object of the present invention is to provide a DNA fragment encoding a nitrogen fixation enzyme that allows Escherichia coli to proliferate without adding metal elements such as V or Mo to the culture medium, a recombinant vector containing the DNA fragment, a transformant transformed by the recombinant vector, and a nitrogen fixation enzyme.

[0012] Solutions for solving problems

[0013] According to one embodiment of the present invention, a DNA fragment, a recombinant vector, a transformant and a nitrogen fixation enzyme as defined by the following [1] to

[12] are provided. The nitrogen fixation enzyme used herein refers to an enzyme that promotes the proliferation (growth) of Escherichia coli in a culture medium that does not contain any nitrogen source (ammonium chloride, sodium nitrate, etc.).

[0014] [1] A DNA fragment encoding a nitrogen-fixing enzyme, comprising the base sequence of SEQ ID NO: 1 or a base sequence having an identity of 50% or more to SEQ ID NO: 1.

[0015] [2] The DNA fragment according to [1], wherein the DNA fragment comprising the base sequence of SEQ ID NO: 1 is derived from genomic DNA of cyanobacteria.

[0016] [3] A DNA fragment encoding a nitrogen fixation enzyme, comprising any one or more of the base sequences of SEQ ID NOs: 2 to 33.

[0017] [4] The DNA fragment according to [3], wherein the DNA fragment is derived from genomic DNA of cyanobacteria.

[0018] [5] The DNA fragment according to [2] or [4], wherein the cyanobacteria includes Cyanothece sp. ATCC 51142.

[0019] [6] A recombinant vector comprising the DNA fragment according to any one of [1] to [5].

[0020] [7] The recombinant vector according to [6], comprising a fosmid vector containing the DNA fragment introduced therein.

[0021] [8] A transformant transformed with the recombinant vector according to [6] or [7].

[0022] [9] A nitrogen fixation enzyme expressed by the transformant according to [8].

[0023]

[10] A nitrogen-fixing enzyme comprising an amino acid sequence identical to the nitrogen-fixing enzyme according to [9], or an amino acid sequence having an identity of 40% or more to the amino acid sequence.

[0024]

[11] The nitrogen fixation enzyme according to [9] or

[10] , comprising any one or more of the amino sequences of SEQ ID NOs: 34 to 65.

[0025]

[12] A nitrogen fixation enzyme comprising any one or more of the amino sequences of SEQ ID NOs: 34 to 65.

[0026] Effects of the Invention

[0027] According to one embodiment of the present invention, a DNA fragment encoding a nitrogen fixation enzyme that allows elimination of the need to add a required nitrogen source to a medium for proliferation of Escherichia coli, a recombinant vector containing the DNA fragment, a transformant transformed by the recombinant vector, and the nitrogen fixation enzyme can be provided.

[0028] Furthermore, according to one embodiment of the present invention, a DNA fragment encoding a nitrogen fixation enzyme that allows Escherichia coli to proliferate without adding a metal element such as V or Mo to the culture medium, a recombinant vector containing the DNA fragment, a transformant transformed by the recombinant vector, and the nitrogen fixation enzyme can be provided. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is an explanatory diagram showing the position of the DNA fragment in the embodiment of the present invention in the genomic DNA and the 32 open reading frames contained in the DNA fragment.

[0030] Figure 2 This is an explanatory diagram showing the position in the fosmid vector where the DNA fragment according to the embodiment of the present invention is introduced.

[0031] Figure 3 This is a diagram showing the evaluation results of the nitrogen fixation ability in Examples. DETAILED DESCRIPTION

[0032] (DNA fragment)

[0033] Figure 1 It is an explanatory diagram showing the position of the DNA fragment in the embodiment of the present invention in the genomic DNA and the 32 open reading frames contained in the DNA fragment.

[0034] The DNA fragment 2 in the embodiment of the present invention encoding the nitrogen fixing enzyme has the base sequence of SEQ ID NO: 1, or a base sequence having an identity of 50% or more with SEQ ID NO: 1. In the embodiment of the present invention, the nitrogen fixing enzyme refers to a nitrogen fixing enzyme that allows the elimination of the need to add a required nitrogen source to the culture medium for the proliferation of Escherichia coli, or a nitrogen fixing enzyme that allows the proliferation of Escherichia coli without adding metal elements such as V or Mo to the culture medium. In a more desirable embodiment of the present invention, it refers to a nitrogen fixing enzyme that is active in oxygen-producing photosynthetic organisms such as algae or plants.

[0035] A DNA fragment having the base sequence of SEQ ID NO: 1 ( Figure 1 2 in the figure) is derived from, for example, genomic DNA of cyanobacteria (also known as blue-green algae) ( Figure 1 1 in the figure) (a 31247 base sequence from the 2982634th to the 3013880th position). The cyanobacteria is, for example, Cyanobacterium cyanobacterium ATCC 51142. Cyanobacterium cyanobacterium ATCC 51142 can be obtained from, for example, the American Type Culture Collection (ATCC).

[0036] The base sequence encoding the nitrogen-fixing enzyme can be a base sequence having an identity of more than 50% with SEQ ID NO:1, and preferably a base sequence having an identity of more than 60% with SEQ ID NO:1, more preferably a base sequence having an identity of more than 70% with SEQ ID NO:1, further preferably a base sequence having an identity of more than 80% with SEQ ID NO:1, further preferably a base sequence having an identity of more than 90% with SEQ ID NO:1, further preferably a base sequence having an identity of more than 95% with SEQID NO:1, and further preferably a base sequence having an identity of more than 98% with SEQ ID NO:1.

[0037] A DNA fragment having the base sequence of SEQ ID NO: 1 or a base sequence having an identity of 50% or more with SEQ ID NO: 1 can be artificially synthesized by genetic engineering methods.

[0038] The DNA fragment encoding the nitrogen fixation enzyme in the embodiment of the present invention has any one or more of the base sequences of SEQ ID NOs: 2 to 33. The base sequences of SEQ ID NOs: 2 to 33 correspond to Figure 1 The open reading frame shown in (gene ID: from cce_2943 to cce_2974). Gene IDs are defined in, for example, CyanoBase ([genome.microbedb.jp / cyanobase / ]) or KEGG, the Kyoto Encyclopedia of Genes and Genomes ([http: / / www.genome.jp / kegg / ]).

[0039] The DNA fragment in the embodiment of the present invention preferably has 5 or more, more preferably 10 or more, further preferably 15 or more, further preferably 20 or more, further preferably 25 or more, further preferably 28 or more, and further preferably 30 or more of the base sequences of SEQ ID NOs: 2 to 33. The order of the base sequences of SEQ ID NOs: 2 to 33 may be changed, but preferably not changed.

[0040] Figure 1 The DNA fragment 2 in the embodiment of the present invention shown in has the base sequence of SEQ ID NO: 1 and also has all of the base sequences of SEQ ID NOs: 2 to 33. The DNA fragment 2 in the embodiment of the present invention may have a base sequence other than the base sequences of SEQ ID NOs: 2 to 33, and may have, for example, a base sequence of tRNA (gene ID: cce_RNA037).

[0041] The DNA fragment having any one or more of the base sequences of SEQ ID NOs: 2 to 33 is derived from, for example, the genomic DNA of cyanobacteria. The cyanobacteria is, for example, Cyanobacterium ATCC 51142.

[0042] Can be according to the following operation steps 1 to 5 of routine execution from cyanobacteria genomic DNA separation DNA fragment 2.More specifically, can be according to for example after-mentioned embodiment separation.The process of each operation is not particularly limited and can adopt various known methods.

[0043] 1. Mass culture of cyanobacteria

[0044] 2. Extraction and fragmentation of genomic DNA from cyanobacteria: As a step to increase the purity of the DNA, polysaccharides may be removed after fragmentation.

[0045] 3. Cloning

[0046] (1) Modify the ends of DNA fragments.

[0047] (2) Sorting by size by electrophoresis (for example, using low melting point agarose for macromolecular separation, 18 V, 24 hours).

[0048] (3) Collect DNA fragments of approximately 25 to 40 kb.

[0049] (4) The collected DNA fragments are inserted into a vector (eg, a fosmid vector) to form a vector (recombinant vector) having various DNA fragments.

[0050] 4. Preparation of Transformants

[0051] (1) Vectors containing various DNA fragments are introduced (packaged) into bacteriophages.

[0052] (2) A host such as E. coli is infected with the bacteriophage described in (1) above to obtain E. coli (transformants) harboring vectors containing various DNA fragments.

[0053] (3) The transformant is grown on an agar medium and colonies are obtained.

[0054] 5. Screening

[0055] (1) Escherichia coli that can grow in a medium that does not contain any nitrogen source (for example, nitrogen compounds such as ammonium chloride and sodium nitrate) is selected.

[0056] (2) Extract the vector from Escherichia coli.

[0057] (3) Decode the genetic information of the DNA fragment inserted into the vector.

[0058] The DNA fragment having any one or more of the base sequences of SEQ ID NOs: 2 to 33 can be artificially synthesized by genetic engineering methods.

[0059] (Recombinant vector)

[0060] Figure 2 This is an explanatory diagram showing the position in the fosmid vector where the DNA fragment according to the embodiment of the present invention is introduced.

[0061] The recombinant vector 3 in the embodiment of the present invention contains the above-mentioned DNA fragment in the embodiment of the present invention. The recombinant vector 3 is preferably obtained by introducing (inserting) the DNA fragment into a Fox plasmid vector, but is not limited thereto. It can be obtained by introducing into, for example, a plasmid vector, a cosmid vector or a viral vector, etc.

[0062] The recombinant vector 3 in the embodiment of the present invention can be obtained according to, for example, the aforementioned operation steps for isolating the DNA fragment 2 from the genomic DNA of cyanobacteria.

[0063] (Transformant)

[0064] The transformant in the embodiment of the present invention is obtained by transforming a host such as Escherichia coli using the above-mentioned recombinant vector in the embodiment of the present invention.

[0065] The transformant in the embodiment of the present invention can be obtained according to, for example, the aforementioned operation steps for isolating the DNA fragment 2 from the genomic DNA of cyanobacteria.

[0066] (Nitrogen fixation enzyme)

[0067] The nitrogen fixation enzyme in the embodiment of the present invention is expressed by the above-mentioned transformant in the embodiment of the present invention. The nitrogen fixation enzyme in the embodiment of the present invention preferably has the amino acid sequences of SEQ ID NOs:34 to 65 corresponding to the base sequences of SEQ ID NOs:2 to 33, respectively (see Table 1 below; SEQ ID NO:2 corresponds to SEQ ID NO:34, ... and SEQ ID NO:33 corresponds to SEQ ID NO:65) and is arranged in this order (SEQ ID NO:34 is on the left end side and SEQ ID NO:65 is on the right end side). Any one or more of the amino acid sequences of SEQ ID NOs:34 to 65 may be an amino acid sequence in which one or more amino acids are inserted, substituted, deleted and / or added and which encodes a nitrogen fixation enzyme that is functionally equivalent to the above-mentioned nitrogen fixation enzyme (the same applies to the nitrogen fixation enzyme in another embodiment of the present invention described below). In such a nitrogen fixation enzyme, for example, 1 to 30, preferably 1 to 20, more preferably 1 to 10, further preferably 1 to 5, most preferably 1 to 2 amino acids may be inserted, substituted, deleted and / or added (the same applies to the nitrogen fixation enzyme in another embodiment of the present invention described below).

[0068] Table 1

[0069]

[0070] Alternatively, the nitrogen fixation enzyme in the embodiments of the present invention may have any one or more of the amino sequences of SEQ ID NOs: 34 to 65.

[0071] The nitrogen fixing enzyme in the embodiment of the present invention preferably has 5 or more, more preferably 10 or more, further preferably 15 or more, further preferably 20 or more, further preferably 25 or more, further preferably 28 or more, and further preferably 30 or more of the amino acid sequences of SEQ ID NOs: 34 to 65. The order of the amino acid sequences of SEQ ID NOs: 34 to 65 may be changed, but preferably is not changed.

[0072] In addition, the nitrogen fixation enzyme in another embodiment of the present invention is not limited to the nitrogen fixation enzyme expressed by the above-mentioned transformant in the embodiment of the present invention, as long as it is a nitrogen fixation enzyme having the same amino acid sequence as the above-mentioned nitrogen fixation enzyme in the embodiment of the present invention. For example, it can be, for example, synthesized by a commercially available protein synthesizer.

[0073] The above-mentioned nitrogen-fixing enzyme is not limited to having the same amino acid sequence as the above-mentioned nitrogen-fixing enzyme in the embodiment of the present invention, and may be a nitrogen-fixing enzyme having an amino acid sequence having an identity of 40% or more with the amino acid sequence. Preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 50% or more with the amino acid sequence, more preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 60% or more with the amino acid sequence, further preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 70% or more with the amino acid sequence, further preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 80% or more with the amino acid sequence, further preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 90% or more with the amino acid sequence, further preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 95% or more with the amino acid sequence, and further preferably, the nitrogen-fixing enzyme has an amino acid sequence having an identity of 98% or more with the amino acid sequence.

[0074] A nitrogen-fixing enzyme having an amino acid sequence with an identity of 40% or more to the above-mentioned nitrogen-fixing enzyme can be artificially synthesized using a commercially available protein synthesizer.

[0075] The "identity" of the base sequence or amino acid sequence used in this specification refers to the degree of consistency of the bases or amino acid residues constituting each sequence between the sequences to be compared. Regarding the amino acid sequence, the existence of vacancies and the properties of amino acids are considered (Wilbur, Proc. Natl. Acad. Sci. USA 80: 726-730 (1983)). In order to calculate the identity, BLAST (Altschul: J. Mol. Biol. 215: 403-410 (1990)) or FASTA (Peasron: Methods in Enzymology 183: 63-69 (1990)) etc. can be used, which are commercially available software. Any "identity" value only needs to be a value calculated by a homology search program well known to those skilled in the art, and can be calculated by using, for example, the default (initial setting) parameters in the homology algorithm BLAST (Basic local alignment search tool) of the National Center for Biotechnology Information (NCBI) http: / / www. / ncbi.nlm.nih.gov / BLAST / .

[0076] (Effects of the Embodiments of the Present Invention)

[0077] The following effects are obtained in the embodiments of the present invention.

[0078] (1) A DNA fragment encoding a nitrogen-fixing enzyme that allows elimination of the need to add a desired nitrogen source to a medium for growth of Escherichia coli, a recombinant vector containing the DNA fragment, a transformant transformed with the recombinant vector, and the nitrogen-fixing enzyme can be provided.

[0079] (2) A DNA fragment encoding a nitrogen-fixing enzyme that allows Escherichia coli to proliferate without adding a metal element such as V or Mo to a culture medium, a recombinant vector containing the DNA fragment, a transformant transformed with the recombinant vector, and the nitrogen-fixing enzyme can be provided.

[0080] (3) Expression of nitrogen fixation enzyme (which is a nitrogen fixation enzyme different from nitrogenase) using a gene derived from cyanobacteria, which is a photosynthetic organism that produces oxygen. Therefore, nitrogen fixation can be performed in photosynthetic organisms that produce oxygen, such as algae or plants.

[0081] (4) Compared with the Haber process which requires a large amount of energy under high temperature and high pressure, it is an energy-saving nitrogen fixation reaction that operates at room temperature and pressure, so it can significantly reduce energy costs.

[0082] (5) By introducing the DNA fragment in the embodiments of the present invention into industrially useful bacteria, algae or plants, species that can grow without the need for nitrogen fertilizers (ammonia, ammonium chloride, sodium nitrate, etc.) can be obtained.

[0083] Example

[0084] The present invention will be described in more detail below based on the following examples. However, the present invention is not limited thereto.

[0085] (Isolation of a DNA fragment encoding the nitrogen-fixing enzyme of the present invention)

[0086] The genomic DNA of the blue rod algae ATCC 51142 (ATCC number (accession number): 51142) extracted from cyanobacteria was physically sheared. Specifically, the extracted genomic DNA solution was aspirated 5 times with a fine-tip pipette tip (from Quality Scientific Plastics, Inc.) and flat ends were produced by End-Repair Enzyme Mix (from Epicentre). Alternatively, vortex or ultrasonic treatment etc. can be used as physical shearing. The flat-terminated DNA fragments were sorted according to size using electrophoresis, and the DNA fragments with an average chain length of 25 to 40 kb were extracted. Then, the DNA fragments were connected to the CopyControl DNA fragments with chloramphenicol resistance gene respectively. TM pCC2FOS TMFox plasmid vector (from Epicentre) (cut and linearized at the Eco72 I site (between position 382C and 383G) and dephosphorylated) (see Figure 2 ). T4 DNA ligase (from TaKaRa) was used to connect the Fox plasmid vector. MaxPlax TM Lambda Packaging Extracts (from Epicentre) were used for in vitro packaging of DNA fragments connected to the Fox plasmid vector. E. coli EPI-300T1R (from Epicentre) (hereinafter referred to as EPI300) as an Escherichia coli was used as a host microorganism. Then, the transformed Escherichia coli (transformant) was obtained in LB medium (LB / Cm) agar plates containing 12.5 mg / mL chloramphenicol.

[0087] Take out the colony of transformed Escherichia coli and place in 4mL of LB medium (LB / Cm) containing 12.5mg / mL chloramphenicol, and cultivate for 18 hours in air at 37°C and 180rpm. Then, collect the cultivated Escherichia coli, wash 3 times with M9-N medium (0.6% Na2HPO4, 0.3% KH2PO4, 0.05% NaCl, 0.2% glucose, 0.00147% CaCl2×2H2O, 0.05% MgSO4×7H2O, 0.01% L-leucine) without any nitrogen source (nitrogen compounds such as ammonium chloride, sodium nitrate), and then resuspend with M9-N medium. Prepared Escherichia coli suspension is placed in M9-N medium (M9-N / Cm) agar plate containing 12.5mg / mL chloramphenicol, and cultivated for 72 hours at 37°C. The obtained colonies were subcultured again on an M9-N medium (M9-N / Cm) agar plate, and a strain showing growth was isolated as a clone having a nitrogen fixation phenotype. This clone was designated as transformant 1.

[0088] Then, the base sequence of the forsch plasmid vector inserted with the clone showing the nitrogen fixation phenotype was analyzed. As a result, it was found that the base sequence of the aforementioned SEQ ID NO:1 was inserted into the transformant 1. In the base sequence, 32 open reading frames containing the base sequences of the aforementioned SEQ ID NOs:2 to 33 and each encoding the amino sequence of the aforementioned SEQ ID NOs:34 to 65 were found. The amino acid sequences of these proteins are not homologous to the known nitrogen fixation proteins (nitrogenase), and therefore the nitrogen fixation enzyme obtained in this example and the DNA fragment encoding the nitrogen fixation enzyme are judged to be new. The base sequence of the forsch plasmid vector inserted was analyzed using pCC2forward-b and pCC2 reverse-b primers. The base sequence of the vector used is as follows;

[0089] pCC2 forward-b; CCAGTCACGACGTTGTTAAAACG

[0090] pCC2 reverse-b;CGCCAAGCTATTTAGGTGAGAC

[0091] (Evaluation of Nitrogen Fixation Ability of Transformants)

[0092] A proliferation test was performed using M9-N medium without a nitrogen source to evaluate the nitrogen fixation ability of transformant 1. Transformant 1 (Example 1) and pCC2FOS without the aforementioned DNA fragment were cultured. TM EPI300 (Comparative Example 1) of the Fox plasmid vector (from Epicentre) was cultured in M9+N medium (M9+N / Cm) obtained by adding 0.1% ammonium chloride as a nitrogen source to M9-N medium for 24 hours, and the bacterial cells were collected, washed 3 times with M9-N medium, and then inoculated in 5 mL of M9-N medium (M9-N / Cm) so that the optical density at a wavelength of 660 nm (hereinafter referred to as OD660) was 0.02. Shaking culture was performed at 37°C and 45 rpm using a small shaking culture device [Bio Photo Recorder (registered trademark) TVS062CA, from Toyo Co., Ltd.], and the OD660 of the culture solution was measured every hour. Figure 3 The proliferation assay results are shown in .

[0093] In the M9-N medium (M9-N / Cm) without a nitrogen source, the proliferation of Escherichia coli EPI-300 (Comparative Example 1) was significantly inhibited, but transformant 1 (Example 1) showed rapid proliferation. This result shows that nitrogen fixation ability can be provided by introducing the DNA fragment encoding the nitrogen fixation enzyme obtained in this example into Escherichia coli.

[0094] The present invention is not limited to the above-described embodiments and examples and can be modified in various ways.

[0095] Description of Reference Numerals

[0096] 1 Genomic DNA

[0097] 2 DNA fragments

[0098] 3 Fox plasmid vector (recombinant vector) Sequence Listing <110> TOYOTA BOSHOKU KABUSHIKI KAISHA <120> DNA Fragment, Recombinant Vector, Transformant, and Nitrogenase <130> PTBOD17456 <160> 65 <170> PatentIn version 3.5 <210> 1 <211> 31247 <212> DNA <213> Cyanothece ATCC51142 <400> 1 acgaaagata atggtggcgg tcctcaaacc atctccattg attaagtcaa ttgactcgat 60 atatcaggag ataggagtta tacatttaac ctatctccta acaccgcagt tctctttccc 120 tgttactttc agactaaatt agggaatatt aaaactagat aaccaaagaa cggtaatctt 180 gacccctttt tgcatccgat tggtcaaaag tccataatgt gacatcggta agagtgaatg 240 agctactgtg taaacccatc ttgcgcccaa cccaaaaacc ccaataatgt ggcggtttgt 300 caatcttgcg gttctcaact gcgattaaat aatcgttatc aacccttagg tattcttgga 360 aaagggggct ttggggccac ctttggggct gcagatattt cccttccagg gaatcccatt 420 tgtgttgtta agcagttacg accagcgaca gacgatcctc aagtctataa aatggctaaa 480 gaattgtttg aacgggaagc agaaacttta ggtaaagtgg gaaatcatcc ccaagtcccc 540 agattattag actattttga acaaaacaaa gaattttatc tggttcaaga atatgtcaaa 600 ggatacaatc ttcatcaaga ggtcaaaaaa aaggggccgt ttagcgaagc aggggttaaa 660 cagttttga cagaactctt acctattcta gaatatatcc attctcagaa agtcattcac 720 cgtgatatca aaccagctaa cctaatccgt tctcaaaaag atagtaaact ggtcttaatt 780 gactttggag cggtcaaaaa tcaggttaac tctatggttg cgaataatac ccaaaccgct 840 tttactgctt ttgccgtggg aacagcaggt ttgcccctc ctgaacaaat ggccatgcgt 900 cctgtttacg cgagtgacat ctatgcagtg ggggttacct gtgtctattt attaaccgct 960 aaaaccccca aagatattgg ttgtgacccc gaaacagggg aaattgcttg ggaaccctat 1020 gttaatatta gtgactcttt ggccaatgtt cttaaaaaga tgttggaagt atcggttaaa 1080 catcgctaca aatcagccga acaagtctta gatgctatgg ccatggcccc ttatgaacaa 1140 gggatgcaag atagcatgac tactatgatc acaggattta aaactcctag tagttcgtcg 1200 agtcccaact cttcactcag taccggttta gtcagttcat ccccatcgac cagaactatg 1260 ggaagagtca acaccatat ttctaaggga agtcccatgt cctcagtttc tccagaggat 1320 aaacactccg tcgctactaa gattcaaggt agggttgcag ggtgtggaaa tgctaactac 1380 aatatagctc atggacaaac ctctagccgt cgtcgtggta aaggacaagg aatctctaac 1440 tctgatacca tagcaactaa aaaaaaccag aaaaaatggc aagaaaaaac cttattaact 1500 gcttatgaaa atggtcgaag agactttact aatcaagaat taaatgaact caatttatct 1560 aaagctttt taccaggaat taattgttat caagccaaat taagccgtat taatttacaa 1620 ggggcagaat taacccgtgc ggatctcgga agagcagatt taacccaagc agtgatgaaa 1680 aatgccaatc ttagtgaggc ttattagga tacgctaact taaatggagc ggatttaaga 1740 ggggcgaatt tatgtggggc caatttaacc tatgctaatt tacaagggac aaatctctgt 1800 ggggttgatt taagttctgc aagaattact gaggctcaat tatctgtagc taaaccaat 1860 tggcgaacag taatgcccag tggtaagcga ggattttggt aatctaatac aaagaatata 1920 atttaagtct ctagtaactt ttgagacttg gtataactcc gaactcacgt tgattgagtc 1980 ctgggagtga acaaaaggta caaacaagga ataaccacta aagcaatgac tgtagccgcc 2040 actaaaccga agataatcgc actacataaa ggcatccaag tgggatcact caaagctaga 2100 ggaattaacc cgataatcgt ggtaatactg gtggttagca caggtcttaa acggtcagca 2160 acccctttgg ctgcggctaa acgtaatttt attccttctt gttgataact attcatggta 2220 tcaaccatga cgatcgcatc attgaccaca attcccacta aagcaataat cccaataaaa 2280 gcggtaaagg aaaaggcgat atctgccagg aaaaagcccc caacggttcc tatcaatgca 2340 aaggggatgg tcagtaaaat gataaagggt tgacgaaatg atcccagttg taacaccagc 2400 actgcaaaga cgagaaaaat agctaaagct aacgcttgac cggcagagcc aaaggtttcc 2460 gcttggtctt ccgtttctcc cccgaaagta taatcatagc cttggggcca agattcttgt 2520 atttcttgta atttgggctg taaatccgct aaaatttccc cagtagtgcg attttcattc 2580 tttgccaaga cggtaacagt gcgctgtcct ccgcgacggg taatagataa aggggcttcg 2640 gcttggactg cttcaatcac tgctcctgcg gaaatcactt cttcctctgg gtttgaggta 2700 aagaaacggg ccgataataa ctcatctcga cgggtgggtc cccccacttc gccgttacgg 2820. gacggccaag cagtgctaag gagaatttct aagtcttcct cattgccacc gatgacaaaa tcaccaatat ctaccgctcg catatatat tgagcttgag aagccagttc ttcttcactg agatcataaa atgacagttc ttctcgcttg ggaattaatc taaggtcagg ttgcaaggct cctaattat ctcgcacatc ggtcgcccca ggtatttgtc tgagggtcat ttgcacttgt tgagaaattt ccctgagttg gcttaattca ctgccggtaa tttcaatttg aatagggtcc ccactttccc ctgcttgttg agcatttaag actaaagagg cgcccggata ttgattaacg gctttttgta attcgttgcg taaatcatct aaatattcaa aggatagccg ttcccgttgg ttttcaggaa ggaacgtacc tgaaaaaccc actagataac tgccttgagt gggttgtagt tccctgatg ccactaatt actgcgctgt cctgtatatt taatcacgct gtcaagatag 3360. tctttttctc gtaaaatttc tcctaggtcg tcggctactt cttgggaac gtctagggtt gtggtggggg gtagttccac attgacacta aagttgcgtt gatcgcctct gggaaaaaat tccacggaaa tttgactaaa taaggtcatg aacagaaaa agagggcaat ggttcctaat 3480 acccaagccc aagccgtttt tttatttcta acggtataat taagactcca attcatccaa 3540 cgagcagacg cggcttgact taaacggtca atggttgat ttttcaac gcttctcata 3600 ttgcctaaca agtaacgaga cagaggaata tcaattaaaa tggcaataac aaaactgagg 3660 actaaacaaa taattgcggt gtagggtaac aattcaataa actttcccaa agtaccgcta 3720 atggccagta agggaatag ggctaaaatg gtggtcaatt gtcctgcaaa agcgggtggt 3780 gcgtaagttt taacggtttt taacccagct tggttaaaag acagtccctc agcaagata 3840 aactcgtgca tccctccat caccaaatg aaaacatcca ccagatacc caggccaag 3900 accataccaa tgagtaccat attattgagg gttgtccgg ttaccataa caccgctaac 3960 gctcccaaaa aggttaaggg aatggatac ccggcaatta aggctcacg ccaaaga 4020 gcaataaaca gcaccacaa cacggcggcc atagcttgca aggcattggt aataaattg 4080 cccaactgtt cccaaataat atccgattca tcggcaataa tttgatattc catgcctctg 4140 ggccacagat tcggattttg ctcaatttgc tccatagcct ctaaaacccg ttcaataacc 4200 tcaatcgaat cttgtccagg tactttttta atacctacac tcaccacagg ttgataagct 4260 tctccttggg tactaataaa ggcacgggtt tcttcttggt caagttctcg acgcacctct 4320 gccagttcac tgaggcgtac tactcgcccc tctaaacgag cgatgggtaa atcttgtaat 4380 gcttctacac tgcgaaaacg agcataaaag cgtacctgtg acccaattaa atcactttct 4440 atctctgaga gggggacatc tcggttagca ttacgaattt ctgtggcaac cgttgtggga 4500 gaaatgccca acgtggctaa acgaaagggg ttcatttgca cattaatgac ctcttctcgc 4560 gctcccccta ggttcacctc actgacccct gccacctgtt ctaaattgtc ttgaatgtcc 4620 tctgctgttt gagaaatcac cgtttgatca atgtccccaa acaaggctaa ggtaaggata 4680 ggggcatcat tgacggaaat ttgttctact acgggttgct ctgcctcttc aggtaattcg 4740 ggttcggctt gggcgacccc ttgccgtaat tcttcgatcg cttcatcggg gtcggcctgg 4800 gacgtaaatt cgacattaat caccgaaaac cctgcataag aagcactttg aacctctttg 4860 acattttcta ctgaagtaat ttctgtttca atttcagaag taacttgttg ttctatggtt 4920 tggggtcgg ctcctggcca ggtggtagtg acggtggcaa cggcaatatc aatatcaggg 4980 ttagactgtt tggtcatgga aaaataaccc aaaagacccc ccaccaccat gagcaaggtt 5040 aataaaatag caaaaatagt gtttaaaaag aaaaatcgtt ctaatcggga ggtttttttg 5100 ccattgactg aggtattatg tccattatta ttgcttctga aatcttcttt taactcactc 5160 attatctacc ttggttaatc acttcaacac tcgccccgtc caccagtcgg ttgtgtcctt 5220 ccgtgaccac taactcccca ggtgttaccc ctgtgataat tccctgttgg gtaatgcctg 5280 taatgcctaa ttctacctct cgctgctcga caatattttc tgcttcgttc accacgaata 5340 cataaggaat gcgatcgcgt cgtacaatgg catcgatggg aacagtgaca gcgttggggt 5400 tttccgacac agcgatccaa gttaacacct gttctccatg tcgtagggtt tcggtggtgc 5460 ttggattaag acgaatggtg gcttctatgg ctcgtcccccc cggactaatg gcaggggttaa 5520 cggcaagac ttcaccccta gcgcgagcgt tagtaattaa ggtctgttga gatgtagccg 5580 cagtattgac ttgggtttct gaggcaatca cagcgttttg accggcttcc acttgttccc 5640 ctgtaggccc ggctaaatct accatgacct cgtattgact cggatcaata ataaccatgg 5700 gaatacgttc taaaatccct tgataatctc ccccgagttg agaggtaacg atttgtggag 5760 agaaatattc tccttctgaa atattgagat aggcaataat gccattaaaa ggtgcataaa 5820 tactggctcc ttctaaagct acctctgctt gagataatcg ggcttcggct gtggtaatgg 5880 cggaggtttg ggcttctacc tgttgttggg ctgcttgtac ttgagcttgg gctgcttcta 5940 cttgttgctg ggctgactgc actcctgctt gagccgactg aacccctgct tgagcatctt 6000 ctaaggtgtt ttgacgttca tcaaattcga tttcagccac tgcaccctct tctacgaggg 6060 ttcgataccg ttctaaactg gttgctgcta atcttcgggc tgcttgagca ttttgtagtt 6120 gggcttgggc ttcttgtact tgcgatcgcg cttgagcaac ctgggcgcga gcttgagcta 6180 cgtctgcagt ggcggccgcc ctgttttgtc gggcttcggc gatcgcagct tgggcttggg 6240 tgacatctgc ggtgagttcg cgatcatcaa tgcgagctaa taattgtcct tttgttacgc 6300 gatcgccttc tcgcagtctt ctaccgtctt ggttagccag ataggtaca tccccttg 6360 tgtcaaggt aagatgttga tagtctacgg ctctacact gccttcactg gagatccaag 6420 cccggagggg ttcccgtgtc gcggtggcaa cctgtactga aagggaact gttcggtga 6480 cagtgacagg ctgatcattg tctcgagac gaatactgt gactccccct aggacgaata 6540 aaaccaaa tactggcaat aaccaccata gttttttaat atttctttc tgctcttttt 6600 tatcagagct ttcggagtt tctttgtatt cttgaggtag atttttgtct agaaattgct 6660 cagtatcatt agatttgg ggtcttgatg gaggatatt tgaggcaaa ggattgtgag 6720 tctgatgatt cataaataa gctatataa atgtctagct agtataata aattagataa 6780 ataataggat ttagcaatta atttaattgt cataatttg tataagcttc aacatcccct 6840 attagacaga ctttgacact ttgctattc aaaaaaca acccaacgga attaaaaaat 6900 ctctaacaat tcatggtttc ccaggggtt ccaaccagc atatagcac tatggttt 6960 ggttaacctg agttcgacgg agagaatct agggaaacct ggacccaac agaagctctc 7020 ataccaaatc cgcttatttt agtagagtaa cttttttctc ccttctccct gctccctgct 7080 tcgagacttg atactatact atctaaagcg gatctcttat caaccctga ttccctcctt 7140 aaaaaaattc taactccgtt gcgtagcacg acgtagtcgc actccgaact cctaaaaccg 7200 aactcacgtt gttaatata gagaattagt ataattttg tactcctacg gtgtaggatg 7260 atatattttt gcttcgacaa aaattttgct tctttat gattatggct gccaaaactg 7320 atgaaattgg ctaaagctga ccctaaaag gttgtttttctacattga acacacctca 7380 accacatcaa tagattagag tcaggttgtg ttataagagt ccctttaaag atgccttgag 7440 gacgaatca aagaatttaa atcatatta aaagctac tcctaattta taatcaggtc 7500 ccaaccaagg tacactcagt tcttgagcaa ctccaatac taagcctcca gcgatcgcac 7560 cataagggtt gccaataccg cctaaaatca cagaggcaaa catgggtaaa attaaaacc 7620 atcccatatt aggtcgtacg gtactggtaa ttaatcccaa catgacaccg cccaaggtgg 7680 ttaaatggc tgtaattacc caagtccaga ggacaacttg ttcacatta attcctgaaa 7740 ccctagctaa atcaatatta tcggctacag ctcgcatggc cttaccaatt tttgtgtttt 7800 gtaacactaa atgtaaaatg gcgatggcta caattgataa aataatcgcc caaattctgt 7860 cggtggctaa ctgtaaccca aaaagttttt gtgcttgaac caagggaata tcataccgtt 7920 gatttttcgc tccccaaatc attaaaatgc cgttacgcaa aaataaggct aagccaatag 7980 agataataat tagggtggtt gaagttgctc tgcgatcgcg catgggtttc cataataaat 8040 attctgaaac taacatagcc aaaacggttc ccatcgcacc gataatcacc gataagccta 8100 aatttaggcc ttgagtgttg gctaaccaag ttaaataggc tcccaaggtc ataaaatctc 8160 cgtgagcgaa gttagaaagc cgtaaaatac catacgtcaa ggttaacccc accgcagcga 8220 gagcaatgac actgccaatg gctaagccat caaataagtt ttgtgctgtc cttaaaatta 8280 gttctgaggt ttccatgctc caaaattgtt gtatcctgcc tgtctcaatg aagatacacc 8340 atcttgagaa agttgctta cttaaatggg gtaaatgggg tgttgagata agttatctct 8400 aattccctct cgttgcactc acgacaactc atggaacaaa attaagcgat cgggatggca 8460 ggatttgaac ctacgacatc ctgctcccaa agcaggcgcg ctaccaagct gcgctacac 8520 ccgtaacgtt gcttacccat tataactca actgtcccta atcaggatac caaaacatcc 8580 ctttaatttcc ttctgggtct agtacaacc ccaacgacg gtaaagtcc accactggg 8640 gatcggcaaa gagggtata ttactatat ctcgcttct taattgacga atcatatact 8700 tcatcatccc tttcccagt cctttacttt ggaacctcgg atgtataacc acatcccata 8760 tagtggcatt aaagcgtga tccgacgtag cgcgagcaaa cccattaaa cgcttccggt 8820 ttccttttac ttcccatgct gatacca taaaactata ggtgagtgcg cgtttaactt 8880 tcctcagagg acgacgagcc catcctaccg catcgcataa ttctcaagt tcatagagat 8940 cgatttctct ttctctgtg aaaaaaatac gcgtttgacc gtcagtgtgg ccttttaagt 9000 tgaccggctc ccctttaaac tgggacttt ggtctgtgtt tgatatatct gtattatta 9060 acaatcgttt ccaaaaaacc attagattga agagacaac ccctattag ttaagtattg 9120 tcgcataaaa atgagcaag agtagggagc atatttt tgcacacaa ttatcttta 9180 atattgacgg ggagggggga gacggggtta aattcatagg acaactatga taaataaagg 9240 acaattttaa aaagataaac aaatggcagt caatcgtatt agaattggta aagataaagg 9300 agaattagta cagtctttgg tggatttcaa tggaggagtt ggaccttttc aaacttatgc 9360 tgatgtgatc acctttgcag caacattagg ggcaaagtac aacaaacgca ttcctctgaa 9420 tataatttca aaagaaccgg cacccattag cttagaaatc tttgtttcta gggggtacga 9480 tgccgtgata aaattatgg caattactga aactaatgat cctcatattc tctctcttta 9540 tgacaccgaa gcagaaaacc aaagaattca aatctttgaa gaatacgcca atggtggcct 9600 tgaacaatta caagaagaat taaaaggcat tgtagattat tcagagcatt tattattatt 9660 gttgaattta gaaagattcc ccaataatac cacagaagag gaatttgatt taactcgatt 9720 tctttgaaaa atggacttta ttttttgatg attattcttt tattttgatg atcaataatc 9780 agagtatttg ttgtatttaa taattattt aagaaaaata atgaatccca aagagaacaa 9840 aaaagaattg agacaaaaaa taataaaaaa acgagagaaa ttatcaaaaa tagattggat 9900 gaataagagc aataaactgt gtgaaaatct acaaaattat cctttattgc aaaaatctaa 9960 aacgattcta gcttacttt cagttcgcca agaacctaat ttaatttccc tattttcaac 10020 tgactataat tgggggtttc ctcgctgtgt aaaaaagtct ttaatctggc attcttggcg 10080 cagagaagat ccgctttatac ctgggaaata tggcatttta gaaccggctg cgaatgcgcc 10140 tattttaacc cctgaaaaag ttgatttaat ccttgttcct gctgtggctt gtgattacaa 10200 tggttatcgc ttaggctatg gtggaggttt ctatgatcgc ttcttaaatt ctcctcaatg 10260 gcattctatt cctacgattg gcattcttt tgaatttgcc ctattacctc aacttcctta 10320 cgaaccttgg gatcagaaat tacaagcaat ttgtacagaa aatgaaatca taagcatatc 10380 tacttaagct tatctattaa gatttatgaa gttgcttagc aactcagaat ggttttgtta 10440 atatgaatga taactattct taaactgtag attaacgggt aaaaaataga ttaatttata 10500 cagaaattag tagtaaatcg agggatctaa aaaaattatg aaacatttta gagatgaatg 10560 gatacaagaa tggtgtgaag aaaatggatg gacagattta tttagggaac gttacaatca 10620 ttattgggca tttcctccag gtgcagtgat gcctgaaccc attccctctg aagtattacg 10680 attcattaaa gcaacaaaag gcttttgcgc ggaagaaaga acctggttaa tttcagctat 10740 tttagtctca attatatccg ttgtgttgag ttatttcttc aaaaatccta tgcccattgt 10800 ctttgctttt gcttttgctg ctgttacttc ggctaagtta gaagtcgaag agatttaaaa 10860 ataattaatt atcttaaatt attctcaaaa ttcctaacta catactaagg tctatcagag 10920 tcaaaatatt gttgtagatt agacttattt tgttctctat cagactcaaa atatttctga 10980 atattctgtt tattctcctc ttctttaggc ggtttctctt cagcaggttt aggaggaata 11040 tgggggatca ctaaagaagg ttctgaaccc tcttggttag taggttcttt gggttctgac 11100 acttggggag gtggttcaac tggaatagga acaactttag tgggttctgg aataggggtt 11160 gaagtcggag aaggggaagc ttttggagta ggggaaggtt gtacggttga gttaggagaa 11220 ggttctagag ttggggtagg agttgcttct agtgtggggg aaggttctaa agttggggta 11280 ggagtcgctt ctaaagtagg agatgactgc gatcctggag aacgcctaac tggtcgccga 11340 agtgtcggtc tgggctggga aaactctggt tgagaatttt ctggaacttc tggagactca 11400 ggtgaaaccg aaggggaaga agtcggttgc gatcgctctt tcgtccctaa catgatccct 11460 aacattaacc ctccaatgat agcactggct gccaaaaatg aacctaaggc taacaaggcc 11520 caaggggtgt ctgtctctgt ttctgttgct tctgctggta agttggtagg gatgggagtg 11580 tgggtagaac ttctagatag agataaattt tgttgtccaa tggctatggt agctgccgtg 11640 ggaatagtat ggggaacctg caaagcctct aacatttctt tagcagtaga aaagcgatcg 11700 cgaggatgaa aacgaaccgc gcgatcaatg acgtttccca cattagactg aatatctggg 11760 gcttcttttc gccataacac ctccccagta ttgggatcag tggctaaata ttggggggtt 11820 tttcctgtga gtaaaaacac catggtcagt cccaaactat acaagtcact tgaatttaga 11880 ggacgaccgg ctgcttgttc ggaagccatg taaccagggg ttcccagtcc gacagaataa 11940 ggcgtattcc catccccatg aatcatggta gcgaccgttt ctttaacaat accaaaatca 12000 attaaaacag gttttttatc gtgactgcga ataataatgt tatctggtttt aatatcacgg 12060 tgaataatac gacgactgtg gatatagtca agaacgggta aaatacctat tatatctct 12120 ctaacttgat taggtgacaa attgccttgt cgttgatgaa tttgggttaa ggtttctcct 12180 tcgatccatt cttgtactaa ataaaaatct cctccttcgg aaaaataagc gtataatgtg 12240 ggaatttgag gatgtttttc ccctaattct tccaaaatag ctgcttcttt ggcaaatcgt 12300 tcctttaacc aatcaggaat aacaggagac tgaaccgccg gtttcagttg tttgatcaca 12360 cattttcttt ctgagggcat atgggtgtca atggcgagaa atgtctcccc aaacccccct 12420 tttcccaagg tttcaatgac ttgataacga ttatttagta ataatgacac ggtaccccct 12480 ttattgccct agtcagacaa tcaagcttta ttgtagctaa tcctcatccg taacttgaga 12540 ctcatttct caatgtcagc gaactattgt ttgcggtagt cccattaaaa acagtgatcc 12600 tggttgaaa tattagcatt ctcaacacaa gggacaattt caccgaataa acttgcccac 12660 aaatatatga ttattgttt acagcgattt tcaattgagt ggaaatcta gaatctaac 12720 tcctgcctcc tgcctcaag ctaaatagtt tatatttat gcaatgaaa actggtgtta 12780 gttttgagac tcaactattt tttagcaatc acccaacgg cgtgaacaat accaggaata 12840 taacccaata acgttaacaa atattgatc cagaactggg gtccaatacc cacttgtaga 12900 aaaacacca gtggaggag gataatggca caaataatcc gaacgacatc catattttt 12960 cttaggtata tatacacttg tgattattca tgaaaccaat tagctgtca agatcataat 13020 gttaactctt tactagggaa aattgtaat ttcaataca atcatcagt gatttttccta 13080 attgccacat aaaaaatgag tgtagataat tggattatct ccaataataa taataatt 13140 gattatgtcc tattcactat ctcctcaaga aaagtccaat acacttat tttctttatg 13200 taatcaacgg aaacatcaac ggtattata tagtcagga tctcggttg actactgat 13260 tgatgggcaa gagatcaaac aatctttaaa aggaatgat attgatgat cttttagtgg 13320 ttgtgggtta attattatcg gtgaggaaaa gttacatatt ggtcaattat gtcgtttaag 13380 aaaagggt attgattcca ttttatgtca aattattttgg ttaagagat tagaaaatc 13440 cattactagg ataggcgtta atattttgat taatccgag taattat taattaacta 13500 aaaaatagaa aagaattt tttgaatatt taaataagc gattgaaa aacttgattc 13560 atccctaag ttctatatt tagggaa tagcacatg aggatcaatg taatctcaga 13620 aggagaagga gatatcccta tttactttaa cgatcttat cgtccaacaa tcctaataat 13680 ggacctaaga taaatccaaa cacaatcct cctgcatggg cccataagc cacaccaccg 13740 ccttccattc ctacattagt ttgaacatt aactcgcaa aaccataaat agcttgttgc 13800 aaaaaccaaa atcctaaaaa gagactgcc ggattctaa tcgttgtaat aaaaattcct 13860 agaggaatta agttacac ttgccttgg ggaaccgta aaatgtaagc cccatcacc 13920 cctgcgatcg ccccactagc cccaagaa ggaacctcag aaaggggc aaataccat 13980 tgacttaac cagctaatac cccacaagtg agatagaaaa tcaagtattt aacatgacct 14040 aatttatctt caacgttatt accaaaaatc cacaaaaaaca acatattgcc tgcaatatgt 14100 aaaaaacctg cgtgaagaaa ttgagacgta aataaggtca tccattcagg aatgggatga 14160 taaaaatcga ctccactaaa ccctgcggtt aattgtttag ggacaatggc samaaattga 14220 aaaaattgcg atagttcctc cgaacttaaa ctcagttctt tgaaaaaaac taaaacgtta 14280 agaataatca atccataagt aacataagct gttctttgtg tgggattat atcatttaaa 14340 ggaaccatat ttgagaattt agaatttaga atttagaagt caaaaataaa atgattaatt 14400 ttttgacccc aacttagctt aacatttcat ttgtgctagt ttctagggaa caaaaaaaat 14460 ctccctagac aagctagaga gaagattgtg aaagtgaact aaattgcctc taaggttgga 14520 tttagaagta gattgatcca ccccaataag aatctaattt tggtgccact aaaatataac 14580 cagacacatc ataaagatcg tcttcggtga agtctcttaa ttctggatag atatcagggc 14640 gactaacatt aacgtgtaat tctgtatagt cgtcttctcc gtcgtaactg gtgggatatt 14700 ttaaataatc aaccaaacct aatacattat cacgggggagg ttcagccagg gataagtctt 14760 ctaatcccaa actaacatta ttattggtct tagtttttcc ttgtaagtga cattgggtac 14820 attcacgaat gaatagtctt tgaccatttg tgatctgttc actggttagg gtgattgttt 14880 ccccttcttc gtttaaagga actgtccgag tttcctcggt taattctaag gcattggcgt 14940 tatggatggg aaattgtaaa aagaaaaaga tagcagcgat cgccatcaga atgaatcgtt 15000 tcaccattgt tctcctgcaa attatgattt tgtgggtata ttaatctttg cacgcattac 15060 ggcaatatca acgataccat aattcctgat ccttaaactt cccttctctg gggggaatgg 15120 gggatttttt cagggataaa aatcgattct ctcccctaag caaacttcat aattcttaaa 15180 agaaacttct cataaatgtc ggctacttgt gtcattaatg gttgggaaag gccagaaaag 15240 acacccatgt taaaactcta tcatttgccg atttccttta attctcgtcg tgtttggatt 15300 gctttactgg aaaaaggatt atcgtttgag ttaattccca tgaaactcaa cggcgatcaa 15360 ctaactccag aatttttagc ccttaaccct tttcatcaca tccctgtcct ggtggatgaa 15420 gcgtttagcc tctttgaatc cctggctatc ttagattatc tcgaagcgaa atatcccacc 15480 ccttccctag ttcctagcga cccccaagga ttagggacag ttaagatgat taacttagtg 15540 accctgaatg aattattacc agcaacaacc ccattaattc agcatagtat gggatttatt 15600 accctagacg aacagaggat agccaacact aaagaaaaag ttgctgtcgt tctcaacttt 15660 tttgagacat ctctcggcga tcgctcctat atagttggca ataccttaac gttggctgac 15720 attgtagcag gaacaatggt ggggtttctg cctcagatgg gtgtttcttt atctgcctat 15780 cctcaattaa ccgcttggac aaagcaatta agtcaacggg aaagttggca acaaacggaa 15840 ccccaaccag aagaaattga caaatttcga gaaaccatga agaaattaat ggctcaacgt 15900 ggttcttaag ctagagagtg attatact cttttccctg atcttcttac ctccttactg 15960 gttttttgtt tctagtctcg gattaaagtt cttatgtatt gttaacgaat ttctgcaata 16020 atttaaggtg gactttgtag acgacttaac tctctcgatg gagggaaaag aaaatttag 16080 taatctgctt gtctaaaaca cggttgccat catattcttt taaaacgtac aacctgtaat 16140 tatcgctgag attgacttct caacctatca actaacctag atccatccat caatcgttat 16200 gacacaagta tctggttcat ccgatgtccc cgatatgggg cgtcgccaat ttatgaactt 16260 actcaccttt ggaaccatca ccggagtcgc tgctggtgct ttgtatcctg tggtgaagta 16320 ttttatcccc ccttcgagtg gtggtgccgg cggtggtatt actgctaaag atgccctcgg 16380 taatgacatt atggctagta actttctggc tactcataat ggaggcgatc gcgttttagc 16440 tcaagggtta aaaggtgatc ccacttacct cgtggtagaa ggagaaaatg ctatcgctga 16500 ctacggtatc aacgccgttt gtactcactt aggctgtgta gttccttgga acgccagcga 16560 agacaagttt atctgtccct gtcatggttc tcaatacaat gctcagggta aagtggtaag 16620 aggtcctgcc cctctgtctt tggctttagc ccatgttaat gtgagcgaag acgataaagt 16680 ggtgttcagc gaatggaccg aaactgactt tcgcaccgaa caagatccct ggtgggctta 16740 atttcagtga atagtgaaca gtaaccactc aattgaacgg gtcactggta accgataact 16800 16860. 16860. 16860. 16860. 16860. 16860. 16860. 16860. 16860. 16860 aatatccgat ttttcggcaa tttggtctaa gggtaagcaa attttactgc gatcgctctt gatcgttgca gcaacagttg ccttattcat aggtaacgta caaagtgcta atgcttatcc tttctgggcc caagaaactg ccccagaaac ccctagagaa gcgacagggc gaattgtttg cgctaactgt cacttagcag aaaaagctgc tgaagtagaa attcctcaat ctgttttacc tgatacagta tttaaggctg tagtaaaat tccttacgat ctcgactccc aacaagttct aggagatgga tcaaaaggtg gcctcaatgt gggggctgtt ttaatgttac ctgaagggtt taaaattgct cccgatgagc gcattcctga agaaataaaa gaagagatgg gtagtgtcta cttccagtcc tacagcgaag gtcaagacaa tgtggttctc gtcggtcctt tacctggaga acaatatcaa gagattattt tccctatctt atctccagat ccctctaagg ataaaaatgt taacttcggt aagtatcaag ttcacttagg ggctaaccgt ggccggggtc aaatttatcc cacaggaca cctagcaata ataacgtctt caagcctct aacgctggta ctattagcaa aattaccgat caagaagatg gtagctacat tgtcactatt gcgacggcag aaggagatgt 17580 agaacgaacg atccccgcag gccctcaatt gatggtttct gaaggcatgg aagttgaagc 17640 aggacaagct ttaaccaata atcctaatgt gggtggtttt ggacaaaaag acacagaagt 17700 ggttctccaa agtcctggcc gcattaaagg attaattctc ttcttagctg gtatcatgtt 17760 agcccaaatt ctcttagtga ttaagaa acaagtggaa cgagtacagg ctgctgaaat 17820 gaacttttaa ttaagttggt ttaaccttag tgttactctt ctagttgtaa cagacaggtt 17880 tccccctgtc tgttctttt atgctttaat agtgataatg attcttattg tcgttgtagc 17940 cttaatctat gaaatcttct cagaagtggt tatcaatgct ccttttgact acaatcctag 18000 18060 gtgacaatca agaacaaagc aacagtcaag atcaattaga cattacggtg agtatcattc 18120 cccagaaata ttttgttaag aaaattgggg gcgatctcgt tagggtgaat gtcatggtag 18180 aacaaggggt actccctcat acctatgaac ccaaacctca aaattacaa gcgttaagtg 18240 aagcggaggc ttatattggt attgggattc cttttgaaac cgcatggatg gatcgcatta 18300 aggaaaa cccgaaaatg ttgatggttg actcaacaca aggaattgat cgattaacta 18360 tgatagccca tgatcaccat gaagagaag atcatggtca ccatgaggaa gaaccaccc 18420 ttgatcccca tgtttggtta tctccaagat tggtaaaat tcaagcacaa aaaatttatg 18480 18540 ttttacaaga aattgaggaa ttagaccatc aagttaggaa taacttagct aacttaaaac 18600 aacgtaaatt tattgttttt catccagctt ggggttattt tgccgaggaa tataacttaa 18660 cgcaagtacc cattgaagtg gggggacaag aacctagcgc atcagagtta ggagacttaa 18720 18780 aagctgccaa aaccattgct aaagaaatta acggagaagt cttattaatt agtcctactg 18840 ctgctgattg gtctaacaac ttactagaag tttctcaaac ctttgccaaa gtgttaaaag 18900 aagaaaataa tcaatagtat aaatttatga aaactgaagt cattaatctt agtcatgttt 18960 gggcaaaata taatcataga aatcctattt tagagacat taacttgact attatgaag 19020 gggattttgt cggattaatt ggtcccaatg gaggaggtaa aacaacttta tttaaagtgt 19080 tattaggact gataaaaccc tatcaaggaa ctgtaaaaat tttaggtaat actgttagta 19140 aaggaagacg ttatatcggt tatgttcctc aattggtaga attagatcga gagttccctg 19200 tgcgtgtcgc tgatgttgta cggatgggaa gactcggaaa acggcgatta ttgcagcgtt 19260 atacccctca agatgaaatt atcgtcaatc gtaccttaga acaggtagga atgatagaat 19320 tacgcaatcg ccctattgct gaattatccg gtggccaacg tcaacgagtc tatattgcgc 19380 gagcattggc atcagaaccc cgtatactat tattagacga accaacggct agtgttgatc 19440 cccaacgaca aaccagcatt tacgagttat tgaaagaatt aaatcaattg attactattg 19500 ttatgatttc tcatgatatt ggggcaattt cagcttatgt caaaaccgta ggatgtctta 19560 accatcgcct cttttttcat ggtgatcctc ctctgagtac cgaaacaata gaacaaactt 19620 atcaatgtcc tgtggatttg atcgcacatg gggttcctca tcgggtcctt tctaaccatg 19680 attgtccctt acattatcat gaataattga tatgttatag gaggtaatat aaaaatgaat 19740 ttgagtatta ttaatgttag aaacactttg ggaatcatta caatttgatt tcatgagaaa 19800 tgctcttttt gcagggatat tagttagtat tgcttgtgga attattggca cgtttgttgt 19860 cattaatcgc attgttttta ttagtggagg tattgctcac gctgcgtatg gtggtattgg 19920 cttaggatat ttttttaaga ttaatcctat ttttggagca attttttttg ctttactttc 19980 tgctttaggt atgggtttag tagtgagaaa aacagaacaa agggctgaca gtttgatagg 20040 ggtaatgtgg gctgtaggga tggcgatcgg tattatttta attgatttaa cgccaggata 20100 taaagcggat ttaatgagtt atttatttgg gagtatttta accgtttctc aagaaaattt 20160 aatgatcatg ttggttttag atgtcattat tgtgttggtt gttagtttat tttataaaga 20220 atttttagcc atttcttttg accccacctt tgctatgact cgtaatgttc ctgtggatag 20280 tttatatta ttattagtcg gtgcgatcgc tttaacggtg gtgatgtaa tgcaggttgt 20340 ggggttaata ttagtgattg cattgttgac tattccggct gcgatcgctg gtcaattttt 20400 gaaagacata aatatatta tgttagtttc tattgttattg gggatgttat ttacaacggt 20460 aggattaatg atatcttatt tctttaacgt aacatctggg gcgactatta ttttagttc 20520 tgggactgct tattatta gtttaggggt aaaaacctta caatttagt ttttattacc 20580 ttgtgctata gttcaccaga aggtataaa aaggagac agaaggtaa attagctgct 20640 aaaattgcct ctattccctcg gttagtcaca ttagatta gtgtagaaca aattgctca 20700 gcattatagt tagagattga acagttaga aacattgtta actattaa taagcaatct 20760 tgaagagatc cgcacttact tcgttctct cctagtccgc agtgcgccta ttataattga 20820 caaaatactg tgaaaactga cagcatttt ttcaactgt ttcaacgtt tttacaatt 20880 gccattaatt agtcgtcagg agatagaaaa atgtttagt ttaagtgatc ttagagaaac 20940 aaagtgtat magaaagg aagaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagg 21000 aaaggaaga haagggag agttagcggc taaaattgcc tctattccctc gcttagtcgc 21060 attaggttta actatagaac aaattgctca agcattagaa ttagacattg aacaagttag 21120 taacattgtc gatctaata aaagtgaa tgccgatttc ttgaatagtt aacattaat 21180 attattctaa ttccgaacc cgaaccctga actcccgtta ataacattc gttacaaatg 21240 ctataacacc ggccacccc aatgatacgc taaacaatgc acgcttatta ctggggaagc 21300 cataatgaca ggagctaacg atacacccta tctccttcgt gctgcacgag gagaaatctt 21360 agatcgtcca ccagtttgga tgatgcgaca agctggacgc tacatgaaag tatatcggga 21420 tttacgggat aaatacccca gtttcgtga aagatccgaa atcctgacc ttgccatcga 21480 aatttctctg siaccttggc gagcatttca accgatggg gtcatgt tctctgatat 21540 tttaaccccg ttacctggga tgggtatacc ttcgatatt gtcgaaagca aagggcccgt 21600 tattgatcca cccattcgca ctaaagaaca agtgataac ttacgtcctt tagatccaga 21660 agaatcctta ccctttatca aaactatttt acaaagttta cggcaagaag tgggtaatca 21720 atctactgtg ttaggatttg tcggatcacc ctggactttg gccgcttatg ctattgaagg 21780 aaaaagttct aagaattatg ccatcatcaa gagtatggcg ttttctcaac ctgagatcct 21840 ccatagtttc ttgagcaaaa tagccgatgc gatcgstatt tatgttagat atcagatcga 21900 ctgtggggcg caagtagtac agttgtttga ctcctgggcc ggtcaactga gtccccaaga 21960 ctatgaaact tttgctctac cttatcaaca acaggtggtg cgtcaggtga aagaaactca 22020 tcccgatacc ccgttaattc tttattag cggtagtgct ggtgttttag agagaatggg 22080 acagtctggg gtcgatattg ttagcgttga ctggactgtg gatatggccg aagccagaca 22140 gcgtttaggt agagacatga aggtacaggg gaatattgat ccaggtgttt tatttgggtc 22200 ccaagacttc attaaagcgc gtattcttga cacagtacgc aaagctgggaa gaggtggcca 22260 tatattaaac ttaggtcacg gtgttttagt gggaactcct gaagataatg ttcgttgctt 22320 ctttgaact gcgaagcagg tggattcatt attagctgtt cctgtataac tttaggggt 22380 caacggctgt tgaccccac tggtgtctat ttaaactgtc tattcattgt tattaagcaa 22440 ttagttttt window acattattaa tgacaaaat agcaagcttt ttacataaa 22500 ttgacaatgt gttgtactc ttataaaaa gaggggttac gatggcatc caggctggta 22560 gactgtgatc caagcctcgt aaatgactgc taaaaaacgt aaaaatttg ttaccgaaaa 22620 agtagaacga gtccttgatt gattcaaaaaatcccttta aacaagctat gtcaatacc 22680 cttcaaaac ctgaatttga ggaactccgt ccaggatta aagttccggc caaaaact 22740 atcctaaccc ctcgcttta caccaccgat ttgaagcga tggcaaaat ggatatttca 22800 gtcaacgaag aggaattaca agctattctc gagagtttc gcactgatta taaccgtcat 22860 cattttatcc gtcgtgaaga atttgcccaa acctgggatc atttgatgg ggataccccgt 22920 cgcttgttg tagaatttt agaacgttct tgtacggcgg aatttcagg gttccttctc 22980 tacaggaat taggagacg gttaaaggat aaagtcctg ttttggcgga atgttttacc 23040 ctcatgtccc gtgatgaagc ccgtcacgcc ggtttcctca ataaagcctt atctgatttt 23100 aatatgtctt tggacttagg gtttttaacc aagagtcgga gttatacctt cttccaacct 23160 aagtttattt tctatgccac ctatttatct gaaaaaattg gttattggcg ttatatcact 23220 atttatcgtc acctagaaca acatcccgaa gacagaatct atccgatttt caacttcttt 23280 gaaaactggt gtcaagatga aaaccgtcat ggggactttt ttgatgccat catgaaagca 23340 actcctgaca ttttaaacga ttggaaagcc cgtttgtggt gtcgcttctt cctgttgtct 23400 gtgtttgcaa ccatgtatct caatgatatt caacggtctg atttttatgc gtctattgga 23460 cttgatgcac gggactatga taaatatgtc attgagaaga ccaacgaaac agccggccga 23520 gtgttcccca tcgttttaga tgtagataat cctgagtttt acgacagttt agaagtttgc 23580 gtcaagaaca acgaaaagtt acgagaaatt gacgcttcta atagtccgac tcctgtgaag 23640 ttcttgagaa aattacctgc ttttatttct aatggagttc agttcttaaa actttatttc 23700 atgaaaccca ttcgggttga tcatttagaa ggaactgtcc gttaaaagtt ttgagttagt 23760 ttacgttttg agtacccgct atagcgggtt ttttgtttta atcgggttga caataagcat 23820 tcatttctgt acctaaatct tgttcttttt tacccaattg ttgaacctgt tgctgcatta 23880 atttagcggt atcaatatct ttatccttca atgcagcaac gaattgacgg gttgtatcgg 23940 catttccttg ataaatatca gcgaacccca tttgatattc ttgtaattgt tcatcttcta 24000 tttttaattg tttcatttgt tgggatgttt cttcaaataa atcggctact tgtaagactt 24060 ttttaatatc agttgtttga cgataacttt cactttcttc tgccattttc tgagttaaaa 24120 ggatgatttt ttgacattga gagactttag tttcagcgca acttattaac aataagttta 24180 tcaaaattgt gaatagagat agggttgaag acaggcactt actatttgaa agttttatca 24240 tcgaatgttc ctcacgttta aaagagttct ttgattagaa aaattatgct attcttcctg 24300 gtttgttaaa ctatgtacta aagtatgaat taaataagtt aactgggtga gttgttctct 24360 agactcggtt tgtgcatcga ctagggtttg aatagactca cttaaagcaa aaatctgata 24420 accttgttgt tgaatttgtt gttcttgttg ttgaatttgt tcaactagat tttccatttt 24480 ctccgctaaa ctttctacgg tttcagtggt agcaataacc gtctctccca tacgttctaa 24540 aatcgtttct attttttgag tattttgaac catttttata ttttatattg attatctaaa 24600 ttttaggaa ttgaataga aaatttaagc attaccgtga cgtttttgat gccattgcca 24660 agcatgatta acaatggttt gaagatcaga atattgaggt tgccaaccta aaactgattg 24720 tgctttttga ctacttccca ctaatattgg gacatctccg ggacggcgat cgctttcttt 24780 cactagaaaa tctattcctg ttacttttt agccatatca atcacctctt taactgaaaa 24840 tccattaccg ttccctaaat taaacatttc actttcaccc ccatttaaaa gatattctaa 24900 tcctaaaata tgagcagatg ctaaatcatt gacatgaata taatctctaa tagctgtgcc 24960 atcatgagta tcataatctg tgccaaaaat aaagagatga tctcgttttt ttagtgcagt 25020 tataaagct aggggaatca aatgcgtttc gggggtgtga tcttctccta aattcccaga 25080 aggatcagcc cctgacgcat taaatagcg gagataacg gattttaatc cataagcttg 25140 atcaaaatct ttgagaattt tttctaccat attacktacta gaagcatagg gacttaatgg 25200 gtgattggga tgctgttctg tcatgggaat ttctgaggc attccataaa tcgcacaggt 25260 agagaaaag acaacttgt taatattagc agccatcatc gctctaata aggttaaggt 25320 tcctactaca ttattgat aaaatggc aggagcttga accgattctc ctacagcaat 25380 aaaagcagca aagtgcataa cagcagcaat atttcttgta gaaatagct catctacag 25440 agtacgattc tgagtatcac ccacaatca ttcacttgt aaaaacctt taataatctc 25500 aggatggcca taggaagat tatcaagac atgacatga tagccagctt ttgtagga 25560 tagacagca tgagaaccga tatatccggc ccctcctgtg actaaaatag tggttttggt 25620 gtctgacaca agcactccctc agcataagctc aagaacgatc ctcttagcga tcgcactcg 25680 tgggttttac ttacttagga taggcattt ttacctta gaaaagccaaatcaggat 25740 cattttggtc tattccctct attackgaaaa atctattcat gtcttctata atacttatta 25800 ttacttaacc atgttagata ccctggatct caaattaact ttagataaag agacttataa 25860 aaccgaaata gaagccttaa tgcgacagtt gcgatcgcta caaaaaagact gttgggataa 25920 tcagctacca gtgattattg tcttagaagg gtgggccgca gcagggaagg gaaaattatt 25980 acagaaaacc attggctata tggacccccg tggctttaac gtttatccta ttttagcagc 26040 cacagaacaa gagaaaaaat atccttttct ttggcgattt tggcacaatt tacccccaaa 26100 aggaagtatt ggcatttttt atcacagttg gtacacccat gtcttagaag atcgtttatt 26160 tggaatagaa accgatggca gtattccttt gttaatgaga gatattaacg cttttgagag 26220 acaattggtg gatgatggcg tggctatagc taaattttgg atacatttaa gtcaaaaaga 26280 actcaaaaag cgactcaaaa catatgcctc tgatgagttg gaatcatggc gtgttcgtcc 26340 ggaagattgg caacaagcca aagaatatga tcgctatggc acctttgctg aggaaatgtt 26400 aacctatact agtaccggtc atgcgccttg gacattagta gaaggggact gtaaacgttg 26460 ggcaagggtt aaggtattat ctcagattgt agcggttatt acccaagcct tagaccgtct 26580. aaaactgata ttccatcttt accccctcaa acggagttac aacccacaga accagatttt ttaggaaaaa tagacttagg gttacattta tttaagatg attackaca aaggttacgg gaagcacagg ttaattaag agagttacag ttacaaattt tcaaaaaaaa ggttcctgtt ttagtcttat ttgaaggatg ggacgcagcc ggaaaggag gagcgataa acggttaact gataccttag atccaagaag ttaaagtt catgcttttt ctgcccccac gtctgaagaa ttaaactatc attatttatg gcgattttgg cgacaaattc cagcacaagg aagcatcgga atttttgatc gaagttggta tggaagagta ttagtcgaaa gggtagaagg gtttgcatcg ggcgacgctc tttaaaga attachment ttgaatcgca gttaacggca tcaggatatg tcatcgttaa gttctggtta catattagtt ttgaagaaca attaacgg tttgaagata gaaaaaata cccgtttaa agctataaat taaccgatga agactggaga aatcgggaa aatggccatt attackcgtc gcagtcaatc aaatgattgc ccgtaccagt accccctatg ctccctggac cattgtacct ggaaacgata aatattatgc 27240 ccgtgttcat gtgttagaaa cagtgattca tgccattgaa acggagttga aacaacgaga 27300 ttaattctta caactcatga ttgatgatta aataattcat aagtaaaaca tacagtttta 27360 ataggtaaac agcgatcaat aattgatact tggtcactgc ttactgataa gatgaaattg 27420 cgttatattt gctacaaaag actgcaaacc tgagttcgat ataaggaaat tgatagataa 27480 cgtgtcaaaa taggaagtct caaactctga ttccctcgtt aaacaaatcc gaactcctaa 27540 caccgaactc aggttgcaag ggagtgggga gagagataat ttgtagcatt cttaatttct 27600 attcaatata acgtctcact gataacgact agccagtctg agcataaaag agttatagct 27660 attctcgatg acagtacaaa agacaattca gaagttctta gggacattaa cgaactttta 27720 tcttcataag cttgactttc atagaatttt acgtttacat aaaatctgta atcttactga 27780 tgataccaaa tccgctttag aaagtagaca aataattagg agtatttaag ccagtgataa 27840 ttggtcaagt ttttgatttc ttcagtcata ttttggagca gacaacaacg tatggctagg 27900 atatcttcta tctcctcaat tgtttcaaaa tactcattaa ccaaaggctc atctaccaaa 27960 gtccataatc tttcggctgg ttgtagctct ggagaataag ctggtaaaaa ttctactatt 28020 attccattag gaattttaag tttttggctt cgatgccacc ccgcattatc ttcaactaaa 28080 aaaattattt tatctttatt aagtcctacg tcttcagcaa aagcttcata gactaaactt 28140 aaccatttat tgttgactct cggtattaag taccataagg tttttcctgt tttgggttcc 28200 acaaatccat aaacatataa ccattcgtaa cgatgttgta ctatagcttt tggtctttct 28260 cctgttggac tccaaacttt tctaataatc ggctttaatc ctactcgatg ctcatcgaaa 28320 aaccagactt ctacagtagc ttttttagac ttttcttgta actttttgac ttttaaaggt 28380 agatttttct tgaagaattc ttgctctata ctatccaggg taagctcatc taaaatccgc 28440 ttgacagaag gaattgagaa cagtcatcat atagttgttg ttcatagcag cttgcttcat 28500 tttctggcga agactacgtt taaatgtttt ttcttgatgg agaacattaa gagccaatcg 28560 tcttagaaga gcaaagttct gtggactata ttctgaccga atacggcatt gatcttcaga 28620 aaaagtgaca tcgagtgtcc aatgaaggtt attctcaatg ctccaatggg tgcggatcgc 28680 atggcaaagg aattgggcat tgggaggtaa agaggtcaga taaaactgaa tatcgtgggt 28740 agtcttgttc cacaagtgac ggatgcgttc gactacgaca atagtttgca gtccatgcca 28800 ttgttgttgc tggtaaagct ctcccatagc tgctactggt atagcccaaa cgtatcgttt 28860 ctcggtgcgg tggtggccct tggttacact tttgtagtaa tcatgttcaa tgccatccca 28920 gccattgttt tgggtatcag taaaccattg cttgacttga gaaaataaag taggatggtt 28980 agctttgaga gtaacaatgt agtcagcttt ttgccgacaa atttgttgga taatgctggt 29040 ttgagttccc attgcatcaa tggtaatgat cgagcctgta atgtctaata gttcgagtaa 29100 ggcaggaatg gccgtaattt cattggagta gttttcaact ttgacttgtc ctaacaccag 29160 actctgctgg gatgcccacg ctgtgaccgt atgtaaagcg cactgtccgg cgttgcgatc 29220 gtaagaaccc cttaatgttt tgccatcgat ggggataatt tctccttgaa ttgaattcat 29280 tatagattga acccattttt gtaggcattt ttgcagcgat tctggatcaa ttctctcaaa 29340 aactcttcta aatgtgtcat cactcgggat tccgtgaggg agttctagaa actctgataa 29400 ccactcctgt tttgcgatac catagttctc catatcttcc caaccttggg agcctgcaat 29460 gactgccaat atagcgatcg ccaatacatc tttaaggagg tgtttttgc ttctttgtac 29520 tctaggatct tctatttctt tgacatagcc taataaattg ttttggattt catcaataga 29580 tgctatattt cccttttcct tttttttctt actagattgt ttttggtctg caaacccctt 29640 ggccatgttc tgattccata attacgaaag tcctaattgt actttgtttt tttattttaa 29700 gtccctttt gcctcaccta gactttactt tcttagtcaa gtacatttta taccatttta 29760 gatgagctta ccctgcaaaa gagtttaaaa ataatgagca aaaaagaaat ttaaaacgca 29820 gacatagccg aaataatcct aatctgaaaa ttagaacata cgatgcacta attaatcaaa 29880 ttaaagatgc tggacaaatt aattacaaat taccttggtt acaaaagcta aaaataaatt 29940 tattacaac acaatcaag gatttaagtg gctacatag taaagtaagc ctaagccccat 30000 atgtcaaatt agaaaaaatg ttcgagtggg acgactcgaa agctaagata atgaaacca 30060 aacattcagt ttctttccct tttgcaacca gggtttttga tgaccaaat aggttaacag 30120 tcattgataa acggtttaac tattggtgaag tgcgatatat tactctcgga aaatagagc 30180 agcgtgttta tgtcgtagct tacaccatca gaggttcagt ggttcgttg atttcagcaa 30240 gaaaagctaa cagtagagag gtaaaacgtt atgacaacca ttaactacac acctgatccct 30300 gaaaaaaaag ctcaactgag tcaagacaa ttacccgtt tggaact ttctgatgag 30360 gatatagact attcagatat ccctgaatta gatgacaact tctgggaaaa tgctgagata 30420 gtcaatactg atgttactca aaacacggta tcatctaccc taggaattt ttaggcgaaa 30480 cacaccccaa tgggtactat tcaacacta shaggaacga gagatattct gccagagag 30540 ataggatact ggcaatgt agaaactgtg gcgacacaaa tactcagtcg tgccatgtac 30600 tatgaaatac gtccccctat ttttgagcaa acttctttat ttgagagagg gataggcgaa 30660 gcaacggatg ttgttggcaa agaaatgtat actttcagcg atagaggcga tcgctcttta 30720 actctacgtc cggaaggaac tgccggcgtt gtccgtgcct atttacaaaa taatctttat 30780 gcagccggtg gagtgcaacg tttgtggtat tgtggcccga tgtttcgcta cgaaagaccc 30840 caagccggtc gtcaacgaca gtttcatcaa attgggttag aattaatcgg tacagccgat 30900 cctagggcag atgtagaggt cattgcttta gctacagata tcttgaagac gttaggatta 30960 caaagtttaa aattagatat taattccgtc ggcgatcgca acgatagaca aaactacaga 31020 gaagcgttag ttaattactt tctcccctac aaagccgaac ttgataccga ctcccaagat 31080 cgtctacaac gcaacccctt acgcattctc gatagtaaag ataaacggac taaagaaatt 31140 aatcaaaatg cgcctagtat tttagaacat cttggagatg cttctaaaaa acactttgat 31200 caagtgcaac aactgttaac ggatcttggc attgaatata acatcaa 31247 <210> 2 <211> 1665 <212> DNA <213> Cyanothece sp. ATCC51142 <400> 2 atgagctact gtgtaaaccc atcttgcgcc caacccaaaa accccaataa tgtggcggtt 60 tgtcaatctt gcggttctca actgcgatta aataatcgtt atcaaccctt aggtattctt 120 ggaaagggg gctttggggc cacctttggg gctgcagata tttcccttcc agggaatccc 180 atttgtgttg ttaagcagtt acgaccagcg acagacgatc ctcaagtcta taaaatggct 240 aaagaattgt ttgaacggga agcagaaact ttaggtaaag tgggaaatca tccccaagtc 300 cccagattat tagactattt tgaacaaaac aaagaatttt atctggttca agaatatgtc 360 aaaggataca atcttcatca agaggtcaaa aaaaaggggc cgtttagcga agcaggggtt 420 aaacagtttt tgacagaact cttacctatt ctagaatata tccattctca gaaagtcatt 480 caccgtgata tcaaaccagc taacctaatc cgttctcaaa aagatagtaa actggtctta 540 attgactttg gagcggtcaa aaatcaggtt aactctatgg ttgcgaataa tacccaaacc 600 gcttttactg cttttgccgt gggaacagca ggttttgcc ctcctgaaca aatggccatg 660 cgtcctgttt acgcgagtga catctatgca gtggggtta cctgtgtcta tttattaacc 720 gctaaaaccc ccaaagatat tggttgtgac cccgaaacag gggaaattgc ttgggaaccc 780 tatgttaata tagtgactc tttggccaat gttcttaaaa agatgttgga agtatcggtt 840 aaacatcgct acaaatcagc cgaacaagtc ttagatgcta tggccatggc cccttatgaa 900 caagggatgc aagatagcat gactactatg atcacaggat ttaaaactcc tagtagttcg 960 tcgagtccca actcttcact cagtaccggt ttagtcagtt catccccatc gaccagaact 1020 atgggaagag tcaacaccca tatttctaag ggaagtccca tgtcctcagt ttctccagag 1080 gataaacact ccgtcgctac taagattcaa ggtagggttg cagggtgtgg aaatgctaac 1140 tacaatatag ctcatggaca aacctctagc cgtcgtcgtg gtaaaggaca aggaatctct 1200 aactctgata ccatagcaac taaaaaaac cagaaaaaat ggcaagaaaa aaccttatta 1260 actgcttatg aaaatggtcg aagagacttt actaatcaag aattaaatga actcaattta 1320 tctaaagctt ttttaccagg aattaattgt tatcaagcca aattaagccg tattaattta 1380 caaggggcag aattaacccg tgcggatctc ggaagagcag attaaccca agcagtgatg 1440 aaaaatgcca atcttagtga ggcttattta ggatacgcta acttaaatgg agcggattta 1500 agaggggcga atttatgtgg ggccaattta acctatgcta atttacaagg agcaaatctc 1560 tgtggggttg atttaagttc tgcaagaatt actgaggctc aattatctgt agctaaaacc 1620 aattggcgaa cagtaatgcc cagtggtaag cgaggatttt ggtaa 1665 <210> 3 <211> 3198 <212> DNA <213> Cyanothece ATCC51142 <400> 3 atgagtgagt taaaagaaga tttcagaagc aataataatg gacataatac ctcagtcaat 60 ggcaaaaaaa cctcccgatt agaacgattt ttctttttaa acactatttt tgctatttta 120 ttaaccttgc tcatggtggt ggggggtctt ttgggttatt tttccatgac caaacagtct 180 aaccctgata ttgatattgc cgttgccacc gtcactacca cctggccagg agccgacccc 240 caaaccatag aacaacaagt tacttctgaa attgaaacag aaattacttc agtagaaaat 300 gtcaaagagg ttcaaagtgc ttcttatgca gggttttcgg tgattaatgt cgaatttacg 360 tcccaggccg accccgatga agcgatcgaa gaattacggc aaggggtcgc ccaagccgaa 420 cccgaattac ctgaagaggc agagcaaccc gtagtagaac aaatttccgt caatgatgcc 480 cctatcctta ccttagcctt gtttggggac attgatcaaa cggtgatttc tcaaacagca 540 gaggacattc aagacaattt agaacaggtg gcaggggtca gtgaggtgaa cctaggggga 600 gcgcgagaag aggtcattaa tgtgcaaatg aacccctttc gtttagccac gttgggcatt 660 tctcccacaa cggttgccac agaaattcgt aatgctaacc gagatgtccc cctctcagag 720 atagaaagtg atttaattgg gtcacaggta cgcttttatg ctcgttttcg cagtgtagaa 780 gcattacaag atttacccat cgctcgttta gaggggcgag tagtacgcct cagtgaactg 840 gcagaggtgc gtcgagaact tgaccaagaa gaaacccgtg cctttattag tacccaagga 900 gaagcttatc aacctgtggt gagtgtaggt attaaaaaag tacctggaca agattcgatt 960 gaggttattg aacgggtttt agaggctatg gagcaaattg agcaaaatcc gaatctgtgg 1020 cccagaggca tggaatatca aattattgcc gatgaatcgg atattatttg ggaacagttg 1080 ggcaatttat ttaccaatgc cttgcaagct atggccgccg tgtttgtggt gctgtttatt 1140 gctctttctt ggcgtgaagc cttaattgcc gggttatcca ttcccttaac ctttttggga 1200 gcgttagcgg tgttatggtt aaccggacaa accctcaata atatggtact cattggtatg 1260 gtcttggcct tgggtatctt ggtggatgtt ttcattttgg tgatggaagg gatgcacgag 1320 tttatctttg ctgagggact gtcttttaac caagctgcgt taaaaaccgt taaaacttac 1380 gcaccacccg cttttgcagg acaattgacc accattttag ccctatttcc cttactggcc 1440 attagcggta ctttgggaaa gtttattgaa ttgttaccct acaccgcaat tatttgttta 1500 gtcctcagtt ttgttattgc cattttaatt gatatcctc tgtctcgtta cttgttaggc 1560 aatatgagaa gcgttgaaaa aaaatcaacc attgaccgtt taagtcaagc cgcgtctgct 1620 cgttggatga attggagtct taattatacc gttagaaata aaaaaacggc ttgggcttgg 1680 gtattaggaa ccattgccct ctttttctgt ttcatgacct tatttagtca aatttccgtg 1740 gattttttc ccagaggcga tcaacgcaac tttagtgtca atgtggaact accccccacc 1800 acaaccctag acgtttccca agaagtagcc gacgacctag gagaaatttt acgagaaaaa 1860 gactatcttg acagcgtgat taaatataca ggacagcgca gtaatttagt ggcatcaggg 1920 gaactacaac ccactcaagg cagttatcta gtgggtttt caggtacgtt ccttcctgaa 1980 aaccaacggg aacggctatc ctttgaatat ttagatgatt tacgcaacga attacaaaaa 2040 gccgttaatc aatatccggg cgcctcttta gtcttaaatg ctcaacaagc aggggaaagt 2100 ggggacccta ttcaaattga aattaccggc agtgaattaa gccaactcag ggaaatttct 2160 caacaagtgc aaatgaccct cagacaaata cctggggcga ccgatgtgcg agataattta 2220 ggagccttgc aacctgacct tagattaatt cccaagcgag aagaactgtc attttatgat 2280 ctcagtgaag aagaactggc ttctcaagct caatattata tgcgagcggt agatattggt 2340 gattttgtca tcggtggcaa tgaggaagac ttagaaattc tccttagcac tgcttggccg 2400 tcccgtaacg gcgaagtggg gggacccacc cgtcgagatg agttattatc ggcccgtttc 2460 tttacctcaa acccagagga agaagtgatt tccgcaggag cagtgattga agcagtccaa 2520 gccgaagccc ctttatctat tacccgtcgc ggaggacagc gcactgttac cgtcttggca 2580 aagaatgaaa atcgcactac tggggaaatt ttagcggatt tacagcccaa attacaagaa 2640 atacaagaat cttggcccca aggctatgat tatactttcg ggggagaaac ggaagaccaa 2700 gcggaaacct ttggctctgc cggtcaagcg ttagctttag ctatttttct cgtctttgca 2760 gtgctggtgt tacaactggg atcatttcgt caacccttta tcattttact gaccatcccc 2820 tttgcattga taggaaccgt tgggggcttt ttcctggcag atatcgcctt ttcctttacc 2880 gcttttattg ggattattgc tttagtggga attgtggtca atgatgcgat cgtcatggtt 2940 gataccatga atagttatca acaagaagga ataaaattac gtttagccgc agccaaaggg 3000 gttgctgacc gtttaagacc tgtgctaacc accagtatta ccacgattat cgggttaatt 3060 cctctagctt tgagtgatcc cacttggatg cctttatgta gtgcgattat cttcggttta 3120 gtggcggcta cagtcattgc tttagtggtt attccttgtt tgtacctttt gttcactccc 3180 aggactcaat caacgtga 3198 <210> 4 <211> 1572 <212> DNA <213> Cyanothece ATCC51142 <400> 4 atgaatcatc agactcacaa tccttgccc tcaaatatcc ctccatcaag accccaaaaa 60 tctaatgata ctgagcaatt tctagacaaa aatctaccct cagaatacaa agaaactccg 120 aaaagctctg ataaaaaaga gcagaaaaga aatattaaaa aactatggtg gttattgcca 180 gtatttggtt tattattcgt cctaggggga gtcacagtta ttcgtcttcg agacaatgat 240 cagcctgtca ctgtcaccga aacgtttcc ctttcagtac aggttgccac cgcgacacgg 300 gaacccctcc gggcttggat ctccagtgaa ggcagtgtta gagccgtaga ctatcaacat 360 cttacctttg acacagaagg ggatgttacc tatctggcta accaagacgg tagaagactg 420 cgagaaggcg atcgcgtaac aaaaggacaa ttattagctc gcattgatga tcgcgaactc 480 accgcagatg tcacccaagc ccaagctgcg atcgccgaag cccgacaaaa cagggcggcc 540 gccactgcag acgtagctca agctcgcgcc caggttgctc aagcgcgatc gcaagtacaa 600 gaagcccaag cccaactaca aaatgctcaa gcagcccgaa gattagcagc aaccagttta 660 gaacggtatc gaaccctcgt agaagagggt gcagtggctg aaatcgaatt tgatgaacgt 720 caaaacacct taggagatgc tcaagcagggg gttcagtcgg ctcaagcagg agtgcagtca 780 gcccagcaac aagtagaagc agcccaagct caagtacaag gcccagcaac acaggtagaa 840 gcccaaacct ccgccattac cacagccgaa gcccgattat ctcaagcaga ggtagcttta 900 gaaggagcca gtatttatgc accttttaat ggcattattg cctatctca tatttcagaa 960 ggagaatatt tctctccaca atcgttacc tctcactcg ggggagatta tcaagggatt 1020 ttagaacgta ttcccatggt tattattgat ccgagtcaat acgaggtcat ggtagattta 1080 gccgggccta caggggaaca agtggaagcc ggtcaaacg ctgtgattgc ctcagaaacc 1140 caagtcaata ctgcggctac atctcacag accttaatta ctaacgctcg cgctaggggt 1200 gaagtctttg ccgttaaccc tgccattagt ccggggggac gagccataga agccaccatt 1260 cgtcttaatc caagcaccac cgaaacccta cgacatggag aacaggtgtt aacttggatc 1320 gctgtgtcgg aaaaccccaa cgctgtcact gttcccattcg atgccattgt acgacgcgat 1380 cgcattcctt atgtattcgt gtgaacgaa gcagaaaata ttgtcgagca gcgagaggta 1440 gaattaggca ttacaggcat tacccaacag ggaattatca caggggtaac acctggggag 1500 ttagtggtca cggaaggaca caaccgactg gtggacgggg cgagtgttga agtgattaac 1560 caaggtagat aa 1572 <210> 5 <211> 885 <212> DNA <213> Cyanothece ATCC51142 <400> 5 atggaaacct cagaactaat tttaaggaca gcacaaaact tatttgatgg cttagccatt 60 ggcagtgtca ttgctctcgc tgcggtgggg ttaaccttga cgtatggtat tttacggctt 120 tctaacttcg ctcacggaga ttttatgacc ttgggagcct atttaacttg gttagccaac 180 actcaaggcc taaatttagg cttatcggtg attatcggtg cgatgggaac cgttttggct 240 atgttagttt cagaatattt attatggaaa cccatgcgcg atcgcagagc aacttcaacc 300 accctaatta ttatctctat tggcttagcc ttatttttgc gtaacggcat tttaatgatt 360 tggggagcga aaaatcaacg gtatgatatt cccttggttc aagcacaaaa actttttggg 420 ttacagttag ccaccgacag aatttgggcg attattttat caattgtagc catcgccatt 480 ttacatttag tgttacaaaa cacaaaaatt ggtaaggcca tgcgagctgt agccgataat 540 attgatttag ctagggtttc aggaattaat gttgaacaag ttgtcctctg gacttgggta 600 attacagcca ttttaaccac cttgggcggt gtcatgttgg gattaattac cagtaccgta 660 cgacctaata tgggatggtt tttaatttta cccatgtttg cctctgtgat tttaggcggt 720 attggcaacc cttatggtgc gatcgctgga ggcttagtta ttggagttgc tcaagaactg 780 agtgtacctt ggttgggacc tgattataaa ttaggagtag ctttattaat tatgatttta 840 attcttttga ttcgtcctca aggcatcttt aaagggactc tttaa 885 <210> 6 <211> 525 <212> DNA <213> Cyanothece ATCC51142 <400> 6 atggtttttt ggaaacgatt gtttaataat acagatatat caaacacaga ccaaaagtcc 60 cagtttaaag gggagccggt caacttaaaa ggccacactg acggtcaaac gcgtattttt 120 ttcaccagag aaagagaaat cgatctctat gaacttgaag aattatgcga tgcggtagga 180 tgggctcgtc gtcctctgag gaaagttaaa cgcgcactca cctatagttt tatggtagtg 240 tcagcatggg aagtaaaagg aaaccggaag cgtttaattg ggtttgctcg cgctacgtcg 300 gatcacgcct ttaatgccac tatatgggat gtggttatac atccgaggtt ccaaagtaaa 360 ggactgggaa aagggatgat gaagtatatg attcgtcaat taagaagcga agatattagt 420 aatattaccc tctttgccga tccccaagtg gtggactttt accgtcgttt ggggtttgta 480 ctagacccag aaggaattaa agggatgttt tggtatcctg attag 525 <210> 7 <211> 465 <212> DNA <213> Cyanothece ATCC51142 <400> 7 atggcagtca atcgtattag aattggtaaa gataaaggag aattagtaca gtctttggtg 60 gatttcaatg gaggagttgg accttttcaa acttatgctg atgtgatcac ctttgcagca 120 acattagggg caaagtacaa caaacgcatt cctctgaata taatttcaaa agaaccggca 180 cccattagct tagaaatctt tgtttctagg gggtacgatg ccgtgataaa attattggca 240 attactgaaa ctaatgatcc tcatattctc tctctttatg acaccgaagc agaaaaccaa 300 agaattcaaa tctttgaaga atacgccaat ggtggccttg aacaattaca agaagaatta 360 aaaggcattg tagattattc agagcattta ttattattgt tgaatttaga aagattcccc 420 aataatacca cagaagagga atttgattta actcgatttc tttga 465 <210> 8 <211> 600 <212> DNA <213> Cyanothece ATCC51142 <400> 8 ttgttgtatt taataattat tttaagaaaa ataatgaatc ccaaagagaa caaaaaagaa 60 ttgagacaaa aaataataaa aaaacgagag aaattatcaa aaatagattg gatgaataag 120 agcaataaac tgtgtgaaaa tctacaaaat tatcctttat tgcaaaaatc taaaacgatt 180 ctagcttact tttcagttcg ccaagaacct aatttaattt ccctattttc aactgactat 240 aattgggggt ttcctcgctg tgtaaaaaag tctttaatct ggcattcttg gcgcagagaa 300 gatccgctta tacctgggaa atatggcatt ttagaaccgg ctgcgaatgc gcctatttta 360 acccctgaaa aagttgattt aatccttgtt cctgctgtgg cttgtgatta caatggttat 420 cgcttaggct atggtggagg tttctatgat cgcttcttaa attctcctca atggcattct 480 attcctacga ttggcattat ctttgaattt gccctattac ctcaacttcc ttacgaacct 540 tgggatcaga aattacaagc aatttgtaca gaaaatgaaa tcataagcat atctacttaa 600 <210> 9 <211> 321 <212> DNA <213> Cyanothece ATCC51142 <400> 9 atgaaacatt ttagagatga atggatacaa gaatggtgtg aagaaaatgg atggacagat 60 ttatttaggg aacgttacaa tcattattgg gcatttcctc caggtgcagt gatgcctgaa 120 cccattccct ctgaagtatt acgattcatt aaagcaacaa aaggcttttg cgcggaagaa 180 agaacctggt taatttcagc tattttagtc tcaattatat ccgttgtgtt gagttatttc 240 ttcaaaaatc ctatgcccat tgtctttgct tttgcttttg ctgctgttac ttcggctaag 300 ttagaagtcg aagagattta a 321 <210> 10 <211> 1566 <212> DNA <213> Cyanothece ATCC51142 <400> 10 gtgtcattat tactaaataa tcgttatcaa gtcattgaaa ccttgggaaa aggggggttt 60 ggggagacat ttctcgccat tgacacccat atgccctcag aaagaaaatg tgtgatcaaa 120 caactgaaac cggcggttca gtctcctgtt attcctgatt ggttaaagga acgatttgcc 180 240. aaagaagcag ctattttgga agaattaggg gaaaaacatc ctcaaattcc cacattatac gcttattttt ccgaaggagg agatttttat ttagtacaag aatggatcga aggagaacc ttaacccaaa ttcatcaacg acaaggcaat ttgtcaccta atcaagttag agagatatta ataggtattt tacccgttct tgactatatc cacagtcgtc gtattattca ccgtgatatt 420 480. aaaccagata acattattat tcgcagtcac gataaaaac ctgttttaat tgattttggt attgttaag aaacggtcgc taccatgatt catggggatg ggaatacgcc ttattctgtc ggactggga cccctggtta catggcttcc ccggtcgtcc tctaaattca agtgacttgt atagtttggg actgaccatg gtgtttttac tcacaggaa aaccccccaa tatttagcca ctgatcccaa tactggggag gtgttatggc gaaaagagc cccagatatt cagtctaatg tgggaaacgt cattgatcgc gcggttcgtt ttcatcctcg cgatcgcttt 780 tctactgcta aagaaatgtt agaggctttg caggttcccc atactattcc cacggcagct 840 accatagcca ttggacaaca aaatttatct ctatctaga gttctaccca cactcccatc cctaccaact taccagcaga agcaacagaa acagagacag acaccccttg ggccttgtta 960 gccttaggtt cattttttggc agccagtgct atcattggag ggttaatgtt agggatcatg 1020 ttaggacga aagagcgatc gcaaccgact tcttcccctt cggtttcacc tgagtctcca 1080 gaagttccag aaaattctca accagagttt tcccagccca gaccgacact tcggcgacca 1140 gttaggcgtt ctccaggatc gcagtcatct cttactttag aagcgactcc taccccaact 1200 ttagaacctt cccccacact agaagcaact cctaccccaa ctctagaacc ttctcctaac 1260 tcaaccgtac aaccttcccc tactccaaaa gcttcccctt ctccgacttc aacccctatt 1320 ccagaaccca ctaaagttgt tcctattcca gttgaaccac ctccccaagt gtcagaaccc 1380 aaagaaccta ctaaccaaga gggttcagaa ccttctttag tgatccccca tattcctcct 1440 aaacctgctg aagagaaacc gcctaaagaa gaggagaata aacagaatat tcagaaatat 1500 tttgagtctg atagagaaca aaataagtct aatctacaac aatattttga ctctgataga 1560 ccttag 1566 <210> 11 <211> 183 <212> DNA <213> Cyanothece sp. ATCC51142 <400> 11 gtgtatatat acctaagaaa aaatatggat gtcgttcgga ttatttgtgc cattatcctt 60 cctccactgg gtgtttttct acaagtgggt attggacccc agttctggat caatattttg 120 ttaacgttat tgggttatat tcctggtatt gttcacgccg tttgggtgat tgctaaaaaa 180 tag 183 <210> 12 <211> 345 <212> DNA <213> Cyanothece sp. ATCC51142 <400> 12 ttgattatgt cctattcact atctcctcaa gaaaagtcca atacaacttt attttcttta 60 tgtaatcaac ggaaacatca acggtattat aatagtcaag gatcttcggt tgaactactg 120 attgatgggc aagagatcaa acaatcttta aaaggaatga ttattgatga ttcttttagt 180 ggttgtgggt taattattat cggtgaggaa aagttacata ttggtcaatt atgtcgttta 240 agaataaagg gtattgattc cattttatgt caaattattt ggttaagaag attagaaaaa 300 tccattacta ggataggcgt taaatatttg attaaatccg agtaa 345 <210> 13 <211> 702 <212> DNA <213> Cyanothece sp. ATCC51142 <400> 13 atggttcctt taaatgataa taatcccaca caaagaacag cttatgttac ttatggattg 60 attattctta acgttttagt ttttttcaaa gaactgagtt taagttcgga ggaactatcg 120 caattttttc aatattatgc cattgtccct aaacaattaa ccgcagggtt tagtggagtc 180 gatttttatc atcccattcc tgaatggatg accttattta cgtctcaatt tcttcacgca 240 ggttttttac atattgcagg caatatgttg tttttgtgga tttttggtaa taacgttgaa 300 gataaattag gtcatgttaa atacttgatt ttctatctca cttgtggggt attagctggt 360 ttaagtcaat ggtattttgc cccttattct gaggttcctt ctttggggc tagtggggcg 420 atcgcagggg tgatggggc ttacatttta cggtttcccc aagcgcaagt gttaacctta 480 attcctctag gaatttttat tacaacgatt agaattccgg cagtcttctt tttaggattt 540 tggtttttgc aacaagctat ttatggtttt gcgagtttaa atgttcaaac taatgtagga 600 atggaaggcg gtggtgtggc ttatgggcc catgcaggag gatttgtgtt tggatttatc 660 ttaggtccat tattaggatt gttggacgat aagaatcgtt aa 702 <210> 14 <211> 486 <212> DNA <213> Specification ATCC51142 <400> 14 atggtgaaac gattcattct gatggcgatc gctgctatct ttttcttttt acaatttccc atccataacg ccaatgcctt agattaacc gaggaaactc ggacagttcc tttaaacgaa gagggga caatcaccct aaccagtga cagatcaca atggtcaaag actattcatt cgtgaatgta cccaatgtca cttacaagga aaaactaaga ccaataata tgttagtttg gttagaag acttatccct ggctgaacct ccccgtgata atgtattagg tttggttgat tatttaaaat atcccaccag ttacgacgga gaagacgact atacagaatt acacgttaat gttagtcgcc ctgatatcta tccagaatta agagacttca ccgaagacga tctttatgat gtgtctggtt atattttagt ggcaccaaaa ttagttctt attggggtgg atcaatctac ttctaa 486 <210> 15 <211> 96 <212> DNA <213> Specification ATCC51142 <400> 15 ttgcttaggg gagagaatcg atttttatcc ctgaaaaaat cccccattcc ccccagagaa gggaagttta aggatcagga attatggtat cgttga <210> 16 <211> 714 <212> DNA <213> Specification ATCC51142 <400> 16 atgtcggcta cttgtgtcat taatggttgg gaaaggccag aaaagacacc catgttaaaa ctctatcatt tgccgatttc ctttaattct cgtcgtgttt ggattgcttt actggaaaaa ggattatcgt ttgagttaat tcccatgaaa ctcaacggcg atcaactaac tccagaattt ttagccctta acccttttca tcacatccct gtcctggtgg atgaagcgtt tagcctcttt 240 gatccctgg ctatcttaga ttatctcga gcgaaatatc ccaccccttc cctagttcct agcgaccccc aaggattagg gacagttaag atgattaact tagtgaccct we ttaccagcaa caaccccatt aattcagcat agtattggat ttattaccct agcgaacag aggatagcca acactaaaga aaaagttgct gtcgttctca acttttttga gacatctctc ggcgatcgct cctatatagt tggcaatacc ttaacgttgg ctgacattgt agcaggaaca atggtggggt ttctgcctca gatgggtgtt tctttatctg cctatcctca attaaccgct 600 tggacaaagc aattaagtca acgggaaagt tggcaacaaa cggaacccca accagaagaa 660 tggacaaagc aattaagtca acgggaaagt tggcaacaaa cggaacccca accagaagaa 660 attgacaaat ttcgagaaac catgaagaaa ttaatggctc aacgtggttc ttaa 714 attgacaaat ttcgagaaac catgaagaaa ttaatggctc aacgtggttc ttaa 714 <210> 17<210> 17 <211> 543<211> 543 <212> DNA<212> DNA <213> 蓝杆藻 ATCC51142<213> Cyanothece ATCC51142 <400> 17<400> 17 atgacacaag tatctggttc atccgatgtc cccgatatgg ggcgtcgcca atttatgaac 60 atgacacaag tatctggttc atccgatgtc cccgatatgg ggcgtcgcca atttatgaac 60 ttactcacct ttggaaccat caccggagtc gctgctggtg ctttgtatcc tgtggtgaag 120 ttactcacct ttggaaccat caccggagtc gctgctggtg ctttgtatcc tgtggtgaag 120 tattttatcc ccccttcgag tggtggtgcc ggcggtggta ttactgctaa agatgccctc 180 tattttatcc ccccttcgag tggtggtgcc ggcggtggta ttactgctaa agatgccctc 180 ggtaatgaca ttatggctag taactttctg gctactcata atggaggcga tcgcgtttta 240 ggtaatgaca ttatggctag taactttctg gctactcata atggaggcga tcgcgtttta 240 gctcaagggt taaaaggtga tcccacttac ctcgtggtag aaggagaaaa tgctatcgct 300 gctcaagggt taaaaggtga tcccacttac ctcgtggtag aaggagaaaa tgctatcgct 300 gactacggta tcaacgccgt ttgtactcac ttaggctgtg tagttccttg gaacgccagc 360 gactacggta tcaacgccgt ttgtactcac ttaggctgtg tagttccttg gaacgccagc 360 gaagacaagt ttatctgtcc ctgtcatggt tctcaataca atgctcaggg taaagtggta 420 gaagacaagt ttatctgtcc ctgtcatggt tctcaataca atgctcaggg taaagtggta 420 agaggtcctg cccctctgtc tttggcttta gcccatgtta atgtgagcga agacgataaa 480 agaggtcctg cccctctgtc tttggcttta gcccatgtta atgtgagcga agacgataaa 480 gtggtgttca gcgaatggac cgaaactgac tttcgcaccg aacaagatcc ctggtgggct 540 gtggtgttca gcgaatggac cgaaactgac tttcgcaccg aacaagatcc ctggtgggct 540 taa 543 <210> 18 <211> 912 <212> DNA <213> Cyanobacterium ATCC51142 <400> 18 ttgatcgttg cagcaacagt tgccttattc ataggtaacg tacaaagtgc taatgcttat 60 cctttctggg cccaagaaac tgccccagaa acccctagag aagcgacagg gcgaattgtt 120 tgcgctaact gtcacttagc agaaaaagct gctgaagtag aaattcctca atctgtttta 180 cctgatacag tatttaaggc tgtagtaaaa attccttacg atctcgactc ccaacaagtt 240 ctaggagatg gatcaaaagg tggcctcaat gtgggggctg ttttaatgtt acctgaaggg 300 tttaaaattg ctcccgatga gcgcattcct gaagaaataa aagaagagat gggtagtgtc 360 tacttccagt cctacagcga aggtcaagac aatgtggttc tcgtcggtcc tttacctgga 420 gaacaatatc aagagattat tttccctatc ttatctccag atccctctaa ggataaaaat 480 gttaacttcg gtaagtatca agttcactta ggggctaacc gtggccgggg tcaaatttat 540 cccacaggac aacctagcaa taataacgtc ttcaaagcct ctaacgctgg tactattagc 600 aaaattaccg atcaagaaga tggtagctac attgtcacta ttgcgacggc agaaggagat 660 gtagacgaaa cgatccccgc aggccctcaa ttgatggttt ctgaaggcat ggaagttgaa 720 gcaggacaag ctttaaccaa taatcctaat gtgggtggtt ttggacaaaa agacacagaa 780 gtggttctcc aaagtcctgg ccgcattaaa ggattaattc tcttcttagc tggtatcatg 840 ttagcccaaa ttctcttagt gattaagaag aaacaagtgg aacgagtaca ggctgctgaa 900 atgaactttt aa 912 <210> 19 <211> 99 <212> DNA <213> Cyanothece ATCC51142 <400> 19 gtgataatga ttcttattgt cgttgtagcc ttaatctatg aaatcttctc agaagtggtt 60 atcaatgctc cttttgacta caatcctagg gatgaatag 99 <210> 20 <211> 933 <212> DNA <213> Cyanothece ATCC51142 <400> 20 ttgactacaa tcctagggat gaatagtatc acaggatgta gttcaagcaa tcctgtcgac 60 tctgggatta ataagagtga caatcaagaa caaagcaaca gtcaagatca attagacatt 120 acggtgagta tcattcccca gaatatttt gttaagaaaa ttgggggcga tctcgttagg 240. gtgaatgtca tggtagaaca aggggtactc cctcatacct atgaacccaa acctcaacaa ttacaagcgt tagtgaagc ggaggcttat attggtattg ggattccttt tgaaaccgca tggatggatc gcattaagg agcaaacccg aaaatgttga tggttgactc aacacaagga attgatcgat taactatgat agcccatgat caccatgaag aagagatca tggtcaccat gaggaga ccacccttga tccccatgtt tggttatctc caagattggt aaaaattcaa gcacaaaaaa tttatgaaac tttagtgaaa cttgatccga aacatcagga aacatatcaa actatctca atagtttttt acaagaatt gaggaattag accatcaagt tags ttagctaact taaaacaacg taaatttatt gtttttcatc cagcttgggg ttattttgcc gaggatata acttaacgca agtacccatt gaagtggggg gacaagaacc tagcgcatca gagttaggag acttaataaa agaagctaaa aaagagaata taaagttat atttgctcaa ccagaattaa gtagtcaagc tgccaaaacc attgctaaag aaattaacgg agaagtctta ttaattagtc ctactgctgc tgattggtct aacaacttac tagaagtttc tcaaaccttt 900 gccaaagtgt taaaagaaga aaataatcaa tag 933 <210> 21 <211> 780 <212> DNA <213> Cyanothece ATCC51142 <400> 21 atgaaaactg aagtcattaa tcttagtcat gtttgggcaa aatataatca tagaaatcct 60 attttagaag acattaactt gactatttat gaaggggatt ttgtcggatt aattggtccc 120 aatggaggag gtaaaacaac tttatttaaa gtgttattag gactgataaa accctatcaa 180 ggaactgtaa aaattttagg taatactgtt agtaaaggaa gacgttatat cggttatgtt 240 cctcaattgg tagaattaga tcgagagttc cctgtgcgtg tcgctgatgt tgtacggatg 300 ggaagactcg gaaaacggcg attattgcag cgttataccc ctcaagatga aattatcgtc 360 aatcgtacct tagaacaggt aggaatgata gaattacgca atcgccctat tgctgaatta 420 tccggtggcc aacgtcaacg agtctatatt gcgcgagcat tggcatcaga accccgtata 480 ctattattag acgaaccaac ggctagtgtt gatccccaac gacaaaccag catttacgag 540 ttattgaaag aattaaatca attgattact attgttatga tttctcatga tattggggca 600 atttcagctt atgtcaaaac cgtaggatgt cttaaccatc gcctcttttt tcatggtgat 660 cctcctctga gtaccgaaac aatagaacaa acttatcaat gtcctgtgga tttgatcgca 720 catggggttc ctcatcgggt cctttctaac catgattgtc ccttacatta tcatgaataa 780 <210> 22 <211> 816 <212> DNA <213> Cyanothece ATCC51142 <400> 22 atgttagaaa cactttggga atcattacaa tttgatttca tgagaaatgc tctttttgca 60 gggatattag ttagtattgc ttgtggaatt attggcacgt ttgttgtcat taatcgcatt 120 gtttttatta gtggaggtat tgctcacgct gcgtatggtg gtattggctt aggatatttt 180 tttaagatta atcctatttt tggagcaatt ttttttgctt tactttctgc tttaggtatg 240 ggtttagtag tgagaaaaac agaacaaagg gctgacagtt tgataggggt aatgtgggct 300 gtagggatgg cgatcggtat tattttaatt gatttaacgc caggatataa agcggattta 360 atgagttatt tatttgggag tattttaacc gtttctcaag aaaatttaat gatcatgttg 420 gttttagatg tcattattgt gttggttgtt agtttatttt ataaagaatt tttagccatt 480 tcttttgacc ccacctttgc tatgactcgt aatgttcctg tggatagttt atatttatta 540 ttagtcggtg cgatcgcttt aacggtggtg atggtaatgc aggttgtggg gttaatatta 600 gtgattgcat tgttgactat tccggctgcg atcgctggtc aatttttgaa agacataaaa 660 tatattatgt tagtttctat tgtattgggg atgttattta caacggtagg attaatgata 720 tcttatttct ttaacgtaac atctggggcg actattattt tagtttctgg gactgcttat 780 ttaattagtt taggggtaaa aaccttacaa atttag 816 <210> 23 <211> 129 <212> DNA <213> Synechococcus sp. ATCC51142 <400> 23 ttgtgctata gttcaccaga aggtataaaa caaggaagac aagaaggtaa attagctgct 60 aaaattgcct ctattcctcg gttagtcaca ttagaattaa gtgtagaaca aattgctcaa 120 gcattatag 129 <210> 24 <211> 267 <212> DNA <213> Cyanobacterium ATCC51142 <400> 24 ttgccattaa ttagtcgtca ggagatagaa aaaatgttta gtttaagtga tcttagagaa 60 accaaagtgt atcaagaagc attagaagaa ggaatagaaa aagggataga acagggaata 120 gaacaaggaa gacaagaggg agagttagcg gctaaaattg cctctattcc tcgcttagtc 180 gcattaggtt taactataga acaaattgct caagcattag aattagacat tgaacaagtt 240 agtaacattg tcgaatctaa taaataa 267 <210> 25 <211> 1065 <212> DNA <213> Cyanobacterium ATCC51142 <400> 25 atgacaggag ctaacgatac accctatctc cttcgtgctg cacgaggaga aatcttagat 60 cgtccaccag tttggatgat gcgacaagct ggacgctaca tgaaagtata tcgggattta 120 cgggataaat accccagttt tcgtgaaaga tccgaaaatc ctgaccttgc catcgaaatt 180 tctctgcaac cttggcgagc atttcaaccg gatggggtca ttatgttctc tgatatttta 240 accccgttac ctgggatggg tatacctttc gatattgtcg aaagcaaagg gcccgttatt 300 gatccaccca ttcgcactaa agaacaagtg gataacttac gtcctttaga tccagaagaa 360 tccttaccct tttcaaaac tattttacaa agtttacggc aagaagtggg taatcaatct 420 actgtgttag gatttgtcgg atcaccctgg actttggccg cttatgctat tgaaggaaaa 480 agttctaaga attatgccat catcaagagt atggcgtttt ctcaacctga gatcctccat 540 agttcttga gcaaatagc cgatgcgatc gctatttatg ttagatatca gatcgactgt 600 ggggcgcaag tagtacagtt gtttgactcc tgggccggtc aactgagtcc ccaagactat 660 gaaacttttg ctctacctta tcaacaacag gtggtgcgtc aggtgaaaga aactcatccc 720 gataccccgt taattcttta tattagcggt agtgctggtg ttttagagag aatgggacag 780 tctggggtg atattgttag cgttgactgg actgtggata tggccgaagc cagacagcgt 840 ttaggtagag acatgaaggt acaggggaat attgatccag gtgttttatt tgggtcccaa 900 gacttcatta aagcgcgtat tcttgacaca gtacgcaaag ctggaagagg tggccatata 960 ttaaacttag gtcacggtgt tttagtggga actcctgaag ataatgttcg ttgcttcttt 1020 gaaactgcga agcaggtgga tcattatta gctgttccctg tataa 1065 <210> 26 <211> 1107 <212> DNA <213> ATCC51142 <400> 26 ttgattcaaa aaatccctt taaacaagct atggtcaata cccttcaaa acctgaattt 60 gaggaactcc gtccaggaat taaagttccg gccaaagaaa ctacctaac cccctcgcttt 120 tacaccaccg attttgaagc gatggcaaaa atggatttt cagtcaacga agaggaatta 180 caagctattc tcgaagagtt tcgcactgat tataaccgtc atcatttat ccgtcgtgaa 240 gatttgccc aaacctggga tcatattgat ggggataccc gtcgctgtt tgtagaattt 300 ttagaacgtt cttgtacggc ggaatttca gggttccttc tctacaagga attaggaga 360 cggttaaagg aaaagtcc tgttttggcg gatgtttta ccctcatgtc ccgtgatgaa 420 gcccgtcacg ccggttctcct cataaagcc ttatctgatt ttaatgtc tttggactta 480 gggtttta ccagagtcg gagttatacc ttctccac ctaagtttat tttctatgcc 540 acctatttat ctgaaaaaat tggttattgg cgttatatca ctatttatcg tcacctagaa 600 caacatcccg aagacagaat ctatccgatt ttcaacttct ttgaaaactg gtgtcaagat 660 gaaaaccgtc atggggactt ttttgatgcc atcatgaaag caactcctga cattttaaac 720 gattggaaag cccgtttgtg gtgtcgcttc ttcctgttgt ctgtgtttgc aaccatgtat 780 ctcaatgata ttcaacggtc tgatttttat gcgtctattg gacttgatgc acgggactat 840 gataaatatg tcattgagaa gaccaacgaa acagccggcc gagtgttccc catcgtttta 900 gatgtagata atcctgagtt ttacgacagt ttagaagttt gcgtcaagaa caacgaaaag 960 ttacgagaaa ttgacgcttc taatagtccg actcctgtga agttcttgag aaaattacct 1020 gcttttattt ctaatggagt tcagttctta aaactttatt tcatgaaacc cattcgggtt 1080 gatcatttag aaagactgt ccgttaa 1107 <210> 27 <211> 444 <212> DNA <213> Blueberry ATCC51142 <400> 27 atgataaaac tttcaaatag taagtgcctg tcttcaaccc tatctctatt caaattttg 60 ataaacttat tgttaataag ttgcgctgaa actaaagtct ctcaatgtca aaaaatcatc 120 cttttaactc agaaaatggc agaagaaagt gaaagttatc gtcaaacaac tgatattaaa 180 aaagtcttac aagtagccga tttatttgaa gaaacatccc aacaaatgaa acaattaaaa 240 atagaagatg aacaattaca agaatatcaa atggggttcg ctgatattta tcaaggaaat 300 gccgatacaa cccgtcaatt cgttgctgca ttgaaggata aagatattga taccgctaaa 360 ttaatgcagc aacaggttca acaattgggt aaaaaagaac aagatttagg tacagaaatg 420 aatgcttatt gtcaacccga ttaa 444 <210> 28 <211> 285 <212> DNA <213> Cyanothece ATCC51142 <400> 28 atggttcaaa atactcaaaa aatagaaacg attttagaac gtatgggaga gacggttatt 60 gctaccactg aaaccgtaga aagtttagcg gagaaaatgg aaaatctagt tgaacaaatt 120 caacaacaag aacaacaaat tcaacaacaa ggttatcaga tttttgcttt aagtgagtct 180 attcaaaccc tagtcgatgc acaaaccgag tctagagaac aactcaccca gttaacttat 240 ttaattcata ctttagtaca tagtttaaca aaccaggaag aatag 285 <210> 29 <211> 1011 <212> DNA <213> Cyanothece ATCC51142 <400> 29 gtgcttgtgt cagacaccaa acccactatt ttagtcacag gaggggccgg atatatcggt 60 tctcatgctg ttctatcctt acaaaaagct ggctatcatg tcattgtctt tgataatctt 120 tcctatggcc atcctgagat tattaaagat gttttacaag ttgaattgat tgtgggtgat 180 actcagaatc gtactctgtt agatgagcta ttttctacaa gaaatattgc tgctgttatg 240 cactttgctg cttttattgc tgtaggagaa tcggttcaag ctcctgccat ttattatcaa 300 aataatgtag taggaacctt aaccttatta gaagcgatga tggctgctaa tattaacaag 360 tttgtctttt cttctacctg tgcgatttat ggaatgcctc aagaaattcc catgacagaa 420 cagcatccca atcacccatt aagtccctat gcttctagta agtatatggt agaaaaaatt 480 ctcaaagatt ttgatcaagc ttatggatta aaatccgtta tcttccgcta ttttaatgcg 540 tcaggggctg atccttctgg gaatttagga gaagatcaca cccccgaaac gcatttgatt 600 cccctagctt tattaactgc actaaaaaaa cgagatcatc tctttatttt tggcacagat 660 tatgatactc atgatggcac agctattaga gattatattc atgtcaatga tttagcatct 720 gctcatattt taggattaga atatctttta aatgggggtg aaagtgaaat gtttaattta 780 gggaacggta atggattttc agttaaagag gtgattgata tggctaaaaa agtaacagga 840 atagattttc tagtgaaaga aagcgatcgc cgtcccggag atgtcccaat attagtggga 900 agtagtcaaa aagcacaatc agttttaggt tggcaacctc aatattctga tcttcaaacc 960 attgttaatc atgcttggca atggcatcaa aaacgtcacg gtaatgctta a 1011 <210> 30 <211> 1560 <212> DNA <213> Cyanothece ATCC51142 <400> 30 ttggtctatt ccctctatac tgaaaaatct attcatgtct tctataatac ttattattac 60 ttaaccatgt tagataccct ggatctcaaa ttaactttag ataaagagac ttataaaacc 120 gaaatagaag ccttaatgcg acagttgcga tcgctacaaa aagactgttg ggataatcag 180 ctaccagtga ttattgtctt agaagggtgg gccgcagcag ggaagggaaa attattacag 240 aaaaccattg gctatatgga cccccgtggc tttaacgttt atcctatttt agcagccaca 300 gaacaagaga aaaataatcc tttctttgg cgattttggc acaatttacc cccaaaagga 360 agtattggca ttttca cagttggtac acccatgtct tagagatcg tttatttgga 420 atagaaaccg atggcagtat tcctttgtta atgagagata ttaacgcttt tgagacaca 480 ttggtggatg atggcgtggc tatagctaaa ttttggatac atttaagtca aaagaactc 540 aaaaagcgac tcaaacata tgcctgat gagttggaat catggcgtgt tcgtccggaa 600 gattggcaac aagccaaaga atatgatcgc tatggcacct tgctgagga atgttaacc 660 tatactagta ccggtcatgc gccttggaca ttagtagag gggactgtaa acgttggggca 720 agggttaagg tattactca gattgtagcg gttattaccc aagccttaga ccgtctcaga 780 ctgccaaaaa ctgatattcc atctttacc cctcaaacgg agttacacc cacagaacca 840 gatttttg gaaaaataga cttaggggtta catttttta aagatgatta taaaaaagg 900 ttacgggag cacaggttaa attagagag ttacagttac aaatttca aaaaaaggtt 960 cctgttttag tcttatttga aggatgggac gcagccggaa aaggaggagc gattaaacgg 1020 ttaactgata ccttagatcc agaagttat aaagttcatg ctttttctgc ccccacgtct 1080 gagaattaa actacatta tttatggcga ttttggcgac aaattccagc acaggaagc 1140 atcggaattt ttgatcgaag ttggtatgga agagtattag tcgaagggt agaagggttt 1200 gcatcggaat tggaatggcg acgctcttat aaagaaatta atgagtttga atcgcagtta 1260 acggcatcag gatatgtcat cgttaagttc tggttacata ttagttttga agaacatta 1320 aaacggtttg aagatagaa aaataacccg tttaaaagct aataattac cgatgaagac 1380 tggagaaatc gggaaaaatg gccattatat tacgtcgcag tcaatcaat gattgcccgt 1440 accagtaccc cctatgctcc ctggaccatt gtacctggaa acgaataata ttatgccgt 1500 gttcatgtgt taggaacagt gattcatgcc attgaaacgg agttgaaca acgagattaa 1560 <210> 31 <211> 387 <212> DNA <213> ATCC51142 <400> 31 gtggaaccca aaacaggaaaaccttatgg tacttatac cgagagtca caataaatgg 60 ttaagtttag tctatgaagc tttgctga gacgtaggac ttaaaga taaatatt 120 tttttagttg aagataatgc ggggtggcat cgaagccaaa acttaaaat tcctaatgga 180 ataatagtag aatttttacc agcttattct ccagagctac aaccagccga aagattatgg 240 actttggtag atgagccttt ggttaatgag tattttgaaa cattgagga gatgaagat 300 atcctagcca tacgttgttg tctgctccaa atatgactg aagaatca aaacttgacc 360 aattatcact ggcttaata ctcctaa 387 <210> 32 <211> 1221 <212> DNA <213> ATCC51142 <400> 32 atggccaagg gtttgcaga ccaaaaaaaaaaaaagga aaagggaaat 60 atagcatcta ttgatgaat ccaaacaat ttattaggct atgtcaaga atagaagat 120 cctagagtac aaagaagcaaaaacaccctc cttaagatg tattggcgat cgctatattg 180 gcagtcattg caggctccca aggttgggaa gatatggaga actatggtat cgcaaacag 240 gagtggttat cagagtttct agaactccct cacggaatcc cgagtgatga cacatttaga 300 agagttttg agagaattga tccagaatcg ctgcaaaat gcctacaaaa atgggttca 360 tctataatga attcaatca aggagaatt atccccatcg atggcaaac atttaggggt 420 tcttacgatc gcaacgccgg acagtgcgct ttacatacgg tcacagcgtg ggcatcccag 480 cagagtctgg tgttaggaca agtcaagtt gaaaactact ccaatgaat tacggccatt 540 cctgccttac tcgaactatt agacattaca ggctcgatca ttaccattga tgcaatggga 600 actcaaacca gcattatcca acaaatttgt cggcaaaag ctgactacat tgttactctc 660 aaagctaacc atcctacttt atttctca gtcaagcaat ggtttactga tacccaaac 720 aatggctggg atggcattga acatgattac tacaaagtg taaccaaggg ccaccaccgc 780 accgagaaac gatacgtttg ggctatacca gtagcagcta tgggagct ttaccagcaa 840 caacaatggc atggactgca aactattgtc gtagtcgaac gcatccgtca cttgtggaac 900 aagactaccc acgatattca gttttatctg acctctttac ctcccaatgc ccaattcctt 960 tgccatgcga tccgcaccca ttggagcatt gagaatacc ttcattggac actcgatgtc 1020 actttttctg aagatcaatg ccgtattcgg tcagaatata gtccacagaa ctttgctctt 1080 ctaagacgat tggctcttaa tgttctccat caagaaaaaa catttaaacg tagtcttcgc 1140 cagaaaatga agcaagctgc tatgaacaac aactatatga tgactgttct caattccttc 1200 tgtcaagcgg attttagatg a 1221 <210> 33 <211> 204 <212> DNA <213> Synechococcus sp. ATCC51142 <400> 33 atgacaacca ttaactacac acctgatcct gaaaaaaaag ctcaactgag tcaagaacaa 60 ttatcccgtt tggaagaact ttctgatgag gatatagact attcagatat ccctgaatta 120 gatgacaact tctgggaaaa tgctgagata gtcaatactg atgttactca aaacacggta 180 tcatctaccc taggaaattt ttag 204 <210> 34 <211> 554 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 34 Met Ser Tyr Cys Val Asn Pro Ser Cys Ala Gln Pro Lys Asn Pro Asn 1 5 10 15 Asn Val Ala Val Cys Gln Ser Cys Gly Ser Gln Leu Arg Leu Asn Asn 20 25 30 Arg Tyr Gln Pro Leu Gly Ile Leu Gly Lys Gly Gly Phe Gly Ala Thr 35 40 45 Phe Gly Ala Ala Asp Ile Ser Leu Pro Gly Asn Pro Ile Cys Val Val 50 55 60 Lys Gln Leu Arg Pro Ala Thr Asp Asp Pro Gln Val Tyr Lys Met Ala 65 70 75 80 Lys Glu Leu Phe Glu Arg Glu Ala Glu Thr Leu Gly Lys Val Gly Asn 85 90 95 His Pro Gln Val Pro Arg Leu Leu Asp Tyr Phe Glu Gln Asn Lys Glu 100 105 110 Phe Tyr Leu Val Gln Glu Tyr Val Lys Gly Tyr Asn Leu His Gln Glu 115 120 125 Val Lys Lys Lys Gly Pro Phe Ser Glu Ala Gly Val Lys Gln Phe Leu 130 135 140 Thr Glu Leu Leu Pro Ile Leu Glu Tyr Ile His Ser Gln Lys Val Ile 145 150 155 160 His Arg Asp Ile Lys Pro Ala Asn Leu Ile Arg Ser Gln Lys Asp Ser 165 170 175 Lys Leu Val Leu Ile Asp Phe Gly Ala Val Lys Asn Gln Val Asn Ser 180 185 190 Met Val Ala Asn Asn Thr Gln Thr Ala Phe Thr Ala Phe Ala Val Gly 195 200 205 Thr Ala Gly Phe Ala Pro Pro Glu Gln Met Ala Met Arg Pro Val Tyr 210 215 220 Ala Ser Asp Ile Tyr Ala Val Gly Val Thr Cys Val Tyr Leu Leu Thr 225 230 235 240 Ala Lys Thr Pro Lys Asp Ile Gly Cys Asp Pro Glu Thr Gly Glu Ile 245 250 255 Ala Trp Glu Pro Tyr Val Asn Ile Ser Asp Ser Leu Ala Asn Val Leu 260 265 270 Lys Lys Met Leu Glu Val Ser Val Lys His Arg Tyr Lys Ser Ala Glu 275 280 285 Gln Val Leu Asp Ala Met Ala Met Ala Pro Tyr Glu Gln Gly Met Gln 290 295 300 Asp Ser Met Thr Thr Met Ile Thr Gly Phe Lys Thr Pro Ser Ser Ser 305 310 315 320 Ser Ser Pro Asn Ser Ser Leu Ser Thr Gly Leu Val Ser Ser Ser Pro 325 330 335 Ser Thr Arg Thr Met Gly Arg Val Asn Thr His Ile Ser Lys Gly Ser 340 345 350 Pro Met Ser Ser Val Ser Pro Glu Asp Lys His Ser Val Ala Thr Lys 355 360 365 Ile Gln Gly Arg Val Ala Gly Cys Gly Asn Ala Asn Tyr Asn Ile Ala 370 375 380 His Gly Gln Thr Ser Ser Arg Arg Arg Gly Lys Gly Gln Gly Ile Ser 385 390 395 400 Asn Ser Asp Thr Ile Ala Thr Lys Lys Asn Gln Lys Lys Trp Gln Glu 405 410 415 Lys Thr Leu Leu Thr Ala Tyr Glu Asn Gly Arg Arg Asp Phe Thr Asn 420 425 430 Gln Glu Leu Asn Glu Leu Asn Leu Ser Lys Ala Phe Leu Pro Gly Ile 435 440 445 Asn Cys Tyr Gln Ala Lys Leu Ser Arg Ile Asn Leu Gln Gly Ala Glu 450 455 460 Leu Thr Arg Ala Asp Leu Gly Arg Ala Asp Leu Thr Gln Ala Val Met 465 470 475 480 Lys Asn Ala Asn Leu Ser Glu Ala Tyr Leu Gly Tyr Ala Asn Leu Asn 485 490 495 Gly Ala Asp Leu Arg Gly Ala Asn Leu Cys Gly Ala Asn Leu Thr Tyr 500 505 510 Ala Asn Leu Gln Gly Ala Asn Leu Cys Gly Val Asp Leu Ser Ser Ala 515 520 525 Arg Ile Thr Glu Ala Gln Leu Ser Val Ala Lys Thr Asn Trp Arg Thr 530 535 540 Val Met Pro Ser Gly Lys Arg Gly Phe Trp 545 550 <210> 35 <211> 1065 <212> PRT <213> Cyanobacterium ATCC51142 <400> 35 Met Ser Glu Leu Lys Glu Asp Phe Arg Ser Asn Asn Asn Gly His Asn 1 5 10 15 Thr Ser Val Asn Gly Lys Lys Thr Ser Arg Leu Glu Arg Phe Phe Phe 20 25 30 Leu Asn Thr Ile Phe Ala Ile Leu Leu Thr Leu Leu Met Val Val Gly 35 40 45 Gly Leu Leu Gly Tyr Phe Ser Met Thr Lys Gln Ser Asn Pro Asp Ile 50 55 60 Asp Ile Ala Val Ala Thr Val Thr Thr Thr Trp Pro Gly Ala Asp Pro 65 70 75 80 Gln Thr Ile Glu Gln Gln Val Thr Ser Glu Ile Glu Thr Glu Ile Thr 85 90 95 Ser Val Glu Asn Val Lys Glu Val Gln Ser Ala Ser Tyr Ala Gly Phe 100 105 110 Ser Val Ile Asn Val Glu Phe Thr Ser Gln Ala Asp Pro Asp Glu Ala 115 120 125 Ile Glu Glu Leu Arg Gln Gly Val Ala Gln Ala Glu Pro Glu Leu Pro 130 135 140 Glu Glu Ala Glu Gln Pro Val Val Glu Gln Ile Ser Val Asn Asp Ala 145 150 155 160 Pro Ile Leu Thr Leu Ala Leu Phe Gly Asp Ile Asp Gln Thr Val Ile 165 170 175 Ser Gln Thr Ala Glu Asp Ile Gln Asp Asn Leu Glu Gln Val Ala Gly 180 185 190 Val Ser Glu Val Asn Leu Gly Gly Ala Arg Glu Glu Val Ile Asn Val 195 200 205 Gln Met Asn Pro Phe Arg Leu Ala Thr Leu Gly Ile Ser Pro Thr Thr 210 215 220 Val Ala Thr Glu Ile Arg Asn Ala Asn Arg Asp Val Pro Leu Ser Glu 225 230 235 240 Ile Glu Ser Asp Leu Ile Gly Ser Gln Val Arg Phe Tyr Ala Arg Phe 245 250 255 Arg Ser Val Glu Ala Leu Gln Asp Leu Pro Ile Ala Arg Leu Glu Gly 260 265 270 Arg Val Val Arg Leu Ser Glu Leu Ala Glu Val Arg Arg Glu Leu Asp 275 280 285 Gln Glu Glu Thr Arg Ala Phe Ile Ser Thr Gln Gly Glu Ala Tyr Gln 290 295 300 Pro Val Val Ser Val Gly Ile Lys Lys Val Pro Gly Gln Asp Ser Ile 305 310 315 320 Glu Val Ile Glu Arg Val Leu Glu Ala Met Glu Gln Ile Glu Gln Asn 325 330 335 Pro Asn Leu Trp Pro Arg Gly Met Glu Tyr Gln Ile Ile Ala Asp Glu 340 345 350 Ser Asp Ile Ile Trp Glu Gln Leu Gly Asn Leu Phe Thr Asn Ala Leu 355 360 365 Gln Ala Met Ala Ala Val Phe Val Val Leu Phe Ile Ala Leu Ser Trp 370 375 380 Arg Glu Ala Leu Ile Ala Gly Leu Ser Ile Pro Leu Thr Phe Leu Gly 385 390 395 400 Ala Leu Ala Val Leu Trp Leu Thr Gly Gln Thr Leu Asn Asn Met Val 405 410 415 Leu Ile Gly Met Val Leu Ala Leu Gly Ile Leu Val Asp Val Phe Ile 420 425 430 Leu Val Met Glu Gly Met His Glu Phe Ile Phe Ala Glu Gly Leu Ser 435 440 445 Phe Asn Gln Ala Ala Leu Lys Thr Val Lys Thr Tyr Ala Pro Pro Ala 450 455 460 Phe Ala Gly Gln Leu Thr Thr Ile Leu Ala Leu Phe Pro Leu Leu Ala 465 470 475 480 Ile Ser Gly Thr Leu Gly Lys Phe Ile Glu Leu Leu Pro Tyr Thr Ala 485 490 495 Ile Ile Cys Leu Val Leu Ser Phe Val Ile Ala Ile Leu Ile Asp Ile 500 505 510 Pro Leu Ser Arg Tyr Leu Leu Gly Asn Met Arg Ser Val Glu Lys Lys 515 520 525 Ser Thr Ile Asp Arg Leu Ser Gln Ala Ala Ser Ala Arg Trp Met Asn 530 535 540 Trp Ser Leu Asn Tyr Thr Val Arg Asn Lys Lys Thr Ala Trp Ala Trp 545 550 555 560 Val Leu Gly Thr Ile Ala Leu Phe Phe Cys Phe Met Thr Leu Phe Ser 565 570 575 Gln Ile Ser Val Glu Phe Phe Pro Arg Gly Asp Gln Arg Asn Phe Ser 580 585 590 Val Asn Val Glu Leu Pro Pro Thr Thr Thr Leu Asp Val Ser Gln Glu 595 600 605 Val Ala Asp Asp Leu Gly Glu Ile Leu Arg Glu Lys Asp Tyr Leu Asp 610 615 620 Ser Val Ile Lys Tyr Thr Gly Gln Arg Ser Asn Leu Val Ala Ser Gly 625 630 635 640 Glu Leu Gln Pro Thr Gln Gly Ser Tyr Leu Val Gly Phe Ser Gly Thr 645 650 655 Phe Leu Pro Glu Asn Gln Arg Glu Arg Leu Ser Phe Glu Tyr Leu Asp 660 665 670 Asp Leu Arg Asn Glu Leu Gln Lys Ala Val Asn Gln Tyr Pro Gly Ala 675 680 685 Ser Leu Val Leu Asn Ala Gln Gln Ala Gly Glu Ser Gly Asp Pro Ile 690 695 700 Gln Ile Glu Ile Thr Gly Ser Glu Leu Ser Gln Leu Arg Glu Ile Ser 705 710 715 720 Gln Gln Val Gln Met Thr Leu Arg Gln Ile Pro Gly Ala Thr Asp Val 725 730 735 Arg Asp Asn Leu Gly Ala Leu Gln Pro Asp Leu Arg Leu Ile Pro Lys 740 745 750 Arg Glu Glu Leu Ser Phe Tyr Asp Leu Ser Glu Glu Glu Leu Ala Ser 755 760 765 Gln Ala Gln Tyr Tyr Met Arg Ala Val Asp Ile Gly Asp Phe Val Ile 770 775 780 Gly Gly Asn Glu Glu Asp Leu Glu Ile Leu Leu Ser Thr Ala Trp Pro 785 790 795 800 Ser Arg Asn Gly Glu Val Gly Gly Pro Thr Arg Arg Asp Glu Leu Leu 805 810 815 Ser Ala Arg Phe Phe Thr Ser Asn Pro Glu Glu Glu Val Ile Ser Ala 820 825 830 Gly Ala Val Ile Glu Ala Val Gln Ala Glu Ala Pro Leu Ser Ile Thr 835 840 845 Arg Arg Gly Gly Gln Arg Thr Val Thr Val Leu Ala Lys Asn Glu Asn 850 855 860 Arg Thr Thr Gly Glu Ile Leu Ala Asp Leu Gln Pro Lys Leu Gln Glu 865 870 875 880 Ile Gln Glu Ser Trp Pro Gln Gly Tyr Asp Tyr Thr Phe Gly Gly Glu 885 890 895 Thr Glu Asp Gln Ala Glu Thr Phe Gly Ser Ala Gly Gln Ala Leu Ala 900 905 910 Leu Ala Ile Phe Leu Val Phe Ala Val Leu Val Leu Gln Leu Gly Ser 915 920 925 Phe Arg Gln Pro Phe Ile Ile Leu Leu Thr Ile Pro Phe Ala Leu Ile 930 935 940 Gly Thr Val Gly Gly Phe Phe Leu Ala Asp Ile Ala Phe Ser Phe Thr 945 950 955 960 Ala Phe Ile Gly Ile Ile Ala Leu Val Gly Ile Val Val Asn Asp Ala 965 970 975 Ile Val Met Val Asp Thr Met Asn Ser Tyr Gln Gln Glu Gly Ile Lys 980 985 990 Leu Arg Leu Ala Ala Ala Lys Gly Val Ala Asp Arg Leu Arg Pro Val 995 1000 1005 Leu Thr Thr Ser Ile Thr Thr Ile Ile Gly Leu Ile Pro Leu Ala 1010 1015 1020 Leu Ser Asp Pro Thr Trp Met Pro Leu Cys Ser Ala Ile Ile Phe 1025 1030 1035 Gly Leu Val Ala Ala Thr Val Ile Ala Leu Val Val Ile Pro Cys 1040 1045 1050 Leu Tyr Leu Leu Phe Thr Pro Arg Thr Gln Ser Thr 1055 1060 1065 <210> 36 <211> 523 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 36 Met Asn His Gln Thr His Asn Pro Leu Pro Ser Asn Ile Pro Pro Ser 1 5 10 15 Arg Pro Gln Lys Ser Asn Asp Thr Glu Gln Phe Leu Asp Lys Asn Leu 20 25 30 Pro Ser Glu Tyr Lys Glu Thr Pro Lys Ser Ser Asp Lys Lys Glu Gln 35 40 45 Lys Arg Asn Ile Lys Lys Leu Trp Trp Leu Leu Pro Val Phe Gly Leu 50 55 60 Leu Phe Val Leu Gly Gly Val Thr Val Ile Arg Leu Arg Asp Asn Asp 65 70 75 80 Gln Pro Val Thr Val Thr Glu Thr Val Ser Leu Ser Val Gln Val Ala 85 90 95 Thr Ala Thr Arg Glu Pro Leu Arg Ala Trp Ile Ser Ser Glu Gly Ser 100 105 110 Val Arg Ala Val Asp Tyr Gln His Leu Thr Phe Asp Thr Glu Gly Asp 115 120 125 Val Thr Tyr Leu Ala Asn Gln Asp Gly Arg Arg Leu Arg Glu Gly Asp 130 135 140 Arg Val Thr Lys Gly Gln Leu Leu Ala Arg Ile Asp Asp Arg Glu Leu 145 150 155 160 Thr Ala Asp Val Thr Gln Ala Gln Ala Ala Ile Ala Glu Ala Arg Gln 165 170 175 Asn Arg Ala Ala Ala Thr Ala Asp Val Ala Gln Ala Arg Ala Gln Val 180 185 190 Ala Gln Ala Arg Ser Gln Val Gln Glu Ala Gln Ala Gln Leu Gln Asn 195 200 205 Ala Gln Ala Ala Arg Arg Leu Ala Ala Thr Ser Leu Glu Arg Tyr Arg 210 215 220 Thr Leu Val Glu Glu Gly Ala Val Ala Glu Ile Glu Phe Asp Glu Arg 225 230 235 240 Gln Asn Thr Leu Glu Asp Ala Gln Ala Gly Val Gln Ser Ala Gln Ala 245 250 255 Gly Val Gln Ser Ala Gln Gln Gln Val Glu Ala Ala Gln Ala Gln Val 260 265 270 Gln Ala Ala Gln Gln Gln Val Glu Ala Gln Thr Ser Ala Ile Thr Thr 275 280 285 Ala Glu Ala Arg Leu Ser Gln Ala Glu Val Ala Leu Glu Gly Ala Ser 290 295 300 Ile Tyr Ala Pro Phe Asn Gly Ile Ile Ala Tyr Leu Asn Ile Ser Glu 305 310 315 320 Gly Glu Tyr Phe Ser Pro Gln Ile Val Thr Ser Gln Leu Gly Gly Asp 325 330 335 Tyr Gln Gly Ile Leu Glu Arg Ile Pro Met Val Ile Ile Asp Pro Ser 340 345 350 Gln Tyr Glu Val Met Val Asp Leu Ala Gly Pro Thr Gly Glu Gln Val 355 360 365 Glu Ala Gly Gln Asn Ala Val Ile Ala Ser Glu Thr Gln Val Asn Thr 370 375 380 Ala Ala Thr Ser Gln Gln Thr Leu Ile Thr Asn Ala Arg Ala Arg Gly 385 390 395 400 Glu Val Phe Ala Val Asn Pro Ala Ile Ser Pro Gly Gly Arg Ala Ile 405 410 415 Glu Ala Thr Ile Arg Leu Asn Pro Ser Thr Thr Glu Thr Leu Arg His 420 425 430 Gly Glu Gln Val Leu Thr Trp Ile Ala Val Ser Glu Asn Pro Asn Ala 435 440 445 Val Thr Val Pro Ile Asp Ala Ile Val Arg Arg Asp Arg Ile Pro Tyr 450 455 460 Val Phe Val Val Asn Glu Ala Glu Asn Ile Val Glu Gln Arg Glu Val 465 470 475 480 Glu Leu Gly Ile Thr Gly Ile Thr Gln Gln Gly Ile Ile Thr Gly Val 485 490 495 Thr Pro Gly Glu Leu Val Val Thr Glu Gly His Asn Arg Leu Val Asp 500 505 510 Gly Ala Ser Val Glu Val Ile Asn Gln Gly Arg 515 520 <210> 37 <211> 294 <212> PRT <213> Cyanobacterium ATCC51142 <400> 37 Met Glu Thr Ser Glu Leu Ile Leu Arg Thr Ala Gln Asn Leu Phe Asp 1 5 10 15 Gly Leu Ala Ile Gly Ser Val Ile Ala Leu Ala Ala Val Gly Leu Thr 20 25 30 Leu Thr Tyr Gly Ile Leu Arg Leu Ser Asn Phe Ala His Gly Asp Phe 35 40 45 Met Thr Leu Gly Ala Tyr Leu Thr Trp Leu Ala Asn Thr Gln Gly Leu 50 55 60 Asn Leu Gly Leu Ser Val Ile Ile Gly Ala Met Gly Thr Val Leu Ala 65 70 75 80 Met Leu Val Ser Glu Tyr Leu Leu Trp Lys Pro Met Arg Asp Arg Arg 85 90 95 Ala Thr Ser Thr Thr Leu Ile Ile Ile Ser Ile Gly Leu Ala Leu Phe 100 105 110 Leu Arg Asn Gly Ile Leu Met Ile Trp Gly Ala Lys Asn Gln Arg Tyr 115 120 125 Asp Ile Pro Leu Val Gln Ala Gln Lys Leu Phe Gly Leu Gln Leu Ala 130 135 140 Thr Asp Arg Ile Trp Ala Ile Ile Leu Ser Ile Val Ala Ile Ala Ile 145 150 155 160 Leu His Leu Val Leu Gln Asn Thr Lys Ile Gly Lys Ala Met Arg Ala 165 170 175 Val Ala Asp Asn Ile Asp Leu Ala Arg Val Ser Gly Ile Asn Val Glu 180 185 190 Gln Val Val Leu Trp Thr Trp Val Ile Thr Ala Ile Leu Thr Thr Leu 195 200 205 Gly Gly Val Met Leu Gly Leu Ile Thr Ser Thr Val Arg Pro Asn Met 210 215 220 Gly Trp Phe Leu Ile Leu Pro Met Phe Ala Ser Val Ile Leu Gly Gly 225 230 235 240 Ile Gly Asn Pro Tyr Gly Ala Ile Ala Gly Gly Leu Val Ile Gly Val 245 250 255 Ala Gln Glu Leu Ser Val Pro Trp Leu Gly Pro Asp Tyr Lys Leu Gly 260 265 270 Val Ala Leu Leu Ile Met Ile Leu Ile Leu Leu Ile Arg Pro Gln Gly 275 280 285 Ile Phe Lys Gly Thr Leu 290 <210> 38 <211> 174 <212> PRT <213> Cyanobacterium ATCC51142 <400> 38 Met Val Phe Trp Lys Arg Leu Phe Asn Asn Thr Asp Ile Ser Asn Thr 1 5 10 15 Asp Gln Lys Ser Gln Phe Lys Gly Glu Pro Val Asn Leu Lys Gly His 20 25 30 Thr Asp Gly Gln Thr Arg Ile Phe Phe Thr Arg Glu Arg Glu Ile Asp 35 40 45 Leu Tyr Glu Leu Glu Glu Leu Cys Asp Ala Val Gly Trp Ala Arg Arg 50 55 60 Pro Leu Arg Lys Val Lys Arg Ala Leu Thr Tyr Ser Phe Met Val Val 65 70 75 80 Ser Ala Trp Glu Val Lys Gly Asn Arg Lys Arg Leu Ile Gly Phe Ala 85 90 95 Arg Ala Thr Ser Asp His Ala Phe Asn Ala Thr Ile Trp Asp Val Val 100 105 110 Ile His Pro Arg Phe Gln Ser Lys Gly Leu Gly Lys Gly Met Met Lys 115 120 125 Tyr Met Ile Arg Gln Leu Arg Ser Glu Asp Ile Ser Asn Ile Thr Leu 130 135 140 Phe Ala Asp Pro Gln Val Val Asp Phe Tyr Arg Arg Leu Gly Phe Val 145 150 155 160 Leu Asp Pro Glu Gly Ile Lys Gly Met Phe Trp Tyr Pro Asp 165 170 <210> 39 <211> 154 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 39 Met Ala Val Asn Arg Ile Arg Ile Gly Lys Asp Lys Gly Glu Leu Val 1 5 10 15 Gln Ser Leu Val Asp Phe Asn Gly Gly Val Gly Pro Phe Gln Thr Tyr 20 25 30 Ala Asp Val Ile Thr Phe Ala Ala Thr Leu Gly Ala Lys Tyr Asn Lys 35 40 45 Arg Ile Pro Leu Asn Ile Ile Ser Lys Glu Pro Ala Pro Ile Ser Leu 50 55 60 Glu Ile Phe Val Ser Arg Gly Tyr Asp Ala Val Ile Lys Leu Leu Ala 65 70 75 80 Ile Thr Glu Thr Asn Asp Pro His Ile Leu Ser Leu Tyr Asp Thr Glu 85 90 95 Ala Glu Asn Gln Arg Ile Gln Ile Phe Glu Glu Tyr Ala Asn Gly Gly 100 105 110 Leu Glu Gln Leu Gln Glu Glu Leu Lys Gly Ile Val Asp Tyr Ser Glu 115 120 125 His Leu Leu Leu Leu Leu Asn Leu Glu Arg Phe Pro Asn Asn Thr Thr 130 135 140 Glu Glu Glu Phe Asp Leu Thr Arg Phe Leu 145 150 <210> 40 <211> 199 <212> PRT <213> Blueberry ATCC51142 <400> 40 Leu Leu Tyr Leu Ile Ile Ile Leu Arg Lys Ile Met Asn Pro Lys Glu 1 5 10 15 Asn Lys Lys Glu Leu Arg Gln Lys Ile Ile Lys Lys Arg Glu Lys Leu 20 25 30 Ser Lys Ile Asp Trp Met Asn Lys Ser Asn Lys Leu Cys Glu Asn Leu 35 40 45 Gln Asn Tyr Pro Leu Leu Gln Lys Ser Lys Thr Ile Leu Ala Tyr Phe 50 55 60 Ser Val Arg Gln Glu Pro Asn Leu Ile Ser Leu Phe Ser Thr Asp Tyr 65 70 75 80 Asn Trp Gly Phe Pro Arg Cys Val Lys Lys Ser Leu Ile Trp His Ser 85 90 95 Trp Arg Arg Glu Asp Pro Leu Ile Pro Gly Lys Tyr Gly Ile Leu Glu 100 105 110 Pro Ala Ala Asn Ala Pro Ile Leu Thr Pro Glu Lys Val Asp Leu Ile 115 120 125 Leu Val Pro Ala Val Ala Cys Asp Tyr Asn Gly Tyr Arg Leu Gly Tyr 130 135 140 Gly Gly Gly Phe Tyr Asp Arg Phe Leu Asn Ser Pro Gln Trp His Ser 145 150 155 160 Ile Pro Thr Ile Gly Ile Ile Phe Glu Phe Ala Leu Leu Pro Gln Leu 165 170 175 Pro Tyr Glu Pro Trp Asp Gln Lys Leu Gln Ala Ile Cys Thr Glu Asn 180 185 190 Glu Ile Ile Ser Ile Ser Thr 195 <210> 41 <211> 106 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 41 Met Lys His Phe Arg Asp Glu Trp Ile Gln Glu Trp Cys Glu Glu Asn 1 5 10 15 Gly Trp Thr Asp Leu Phe Arg Glu Arg Tyr Asn His Tyr Trp Ala Phe 20 25 30 Pro Pro Gly Ala Val Met Pro Glu Pro Ile Pro Ser Glu Val Leu Arg 35 40 45 Phe Ile Lys Ala Thr Lys Gly Phe Cys Ala Glu Glu Arg Thr Trp Leu 50 55 60 Ile Ser Ala Ile Leu Val Ser Ile Ile Ser Val Val Leu Ser Tyr Phe 65 70 75 80 Phe Lys Asn Pro Met Pro Ile Val Phe Ala Phe Ala Phe Ala Ala Val 85 90 95 Thr Ser Ala Lys Leu Glu Val Glu Glu Ile 100 105 <210> 42 <211> 521 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 42 Val Ser Leu Leu Leu Asn Asn Arg Tyr Gln Val Ile Glu Thr Leu Gly 1 5 10 15 Lys Gly Gly Phe Gly Glu Thr Phe Leu Ala Ile Asp Thr His Met Pro 20 25 30 Ser Glu Arg Lys Cys Val Ile Lys Gln Leu Lys Pro Ala Val Gln Ser 35 40 45 Pro Val Ile Pro Asp Trp Leu Lys Glu Arg Phe Ala Lys Glu Ala Ala 50 55 60 Ile Leu Glu Glu Leu Gly Glu Lys His Pro Gln Ile Pro Thr Leu Tyr 65 70 75 80 Ala Tyr Phe Ser Glu Gly Gly Asp Phe Tyr Leu Val Gln Glu Trp Ile 85 90 95 Glu Gly Glu Thr Leu Thr Gln Ile His Gln Arg Gln Gly Asn Leu Ser 100 105 110 Pro Asn Gln Val Arg Glu Ile Leu Ile Gly Ile Leu Pro Val Leu Asp 115 120 125 Tyr Ile His Ser Arg Arg Ile Ile His Arg Asp Ile Lys Pro Asp Asn 130 135 140 Ile Ile Ile Arg Ser His Asp Lys Lys Pro Val Leu Ile Asp Phe Gly 145 150 155 160 Ile Val Lys Glu Thr Val Ala Thr Met Ile His Gly Asp Gly Asn Thr 165 170 175 Pro Tyr Ser Val Gly Leu Gly Thr Pro Gly Tyr Met Ala Ser Glu Gln 180 185 190 Ala Ala Gly Arg Pro Leu Asn Ser Ser Asp Leu Tyr Ser Leu Gly Leu 195 200 205 Thr Met Val Phe Leu Leu Thr Gly Lys Thr Pro Gln Tyr Leu Ala Thr 210 215 220 Asp Pro Asn Thr Gly Glu Val Leu Trp Arg Lys Glu Ala Pro Asp Ile 225 230 235 240 Gln Ser Asn Val Gly Asn Val Ile Asp Arg Ala Val Arg Phe His Pro 245 250 255 Arg Asp Arg Phe Ser Thr Ala Lys Glu Met Leu Glu Ala Leu Gln Val 260 265 270 Pro His Thr Ile Pro Thr Ala Ala Thr Ile Ala Ile Gly Gln Gln Asn 275 280 285 Leu Ser Leu Ser Arg Ser Ser Thr His Thr Pro Ile Pro Thr Asn Leu 290 295 300 Pro Ala Glu Ala Thr Glu Thr Glu Thr Asp Thr Pro Trp Ala Leu Leu 305 310 315 320 Ala Leu Gly Ser Phe Leu Ala Ala Ser Ala Ile Ile Gly Gly Leu Met 325 330 335 Leu Gly Ile Met Leu Gly Thr Lys Glu Arg Ser Gln Pro Thr Ser Ser 340 345 350 Pro Ser Val Ser Pro Glu Ser Pro Glu Val Pro Glu Asn Ser Gln Pro 355 360 365 Glu Phe Ser Gln Pro Arg Pro Thr Leu Arg Arg Pro Val Arg Arg Ser 370 375 380 Pro Gly Ser Gln Ser Ser Pro Thr Leu Glu Ala Thr Pro Thr Pro Thr 385 390 395 400 Leu Glu Pro Ser Pro Thr Leu Glu Ala Thr Pro Thr Pro Thr Leu Glu 405 410 415 Pro Ser Pro Asn Ser Thr Val Gln Pro Ser Pro Thr Pro Lys Ala Ser 420 425 430 Pro Ser Pro Thr Ser Thr Pro Ile Pro Glu Pro Thr Lys Val Val Pro 435 440 445 Ile Pro Val Glu Pro Pro Pro Gln Val Ser Glu Pro Lys Glu Pro Thr 450 455 460 Asn Gln Glu Gly Ser Glu Pro Ser Leu Val Ile Pro His Ile Pro Pro 465 470 475 480 Lys Pro Ala Glu Glu Lys Pro Pro Lys Glu Glu Glu Asn Lys Gln Asn 485 490 495 Ile Gln Lys Tyr Phe Glu Ser Asp Arg Glu Gln Asn Lys Ser Asn Leu 500 505 510 Gln Gln Tyr Phe Asp Ser Asp Arg Pro 515 520 <210> 43 <211> 60 <212> PRT <213> Cyanobacterium ATCC51142 <400> 43 Val Tyr Ile Tyr Leu Arg Lys Asn Met Asp Val Val Arg Ile Ile Cys 1 5 10 15 Ala Ile Ile Leu Pro Pro Leu Gly Val Phe Leu Gln Val Gly Ile Gly 20 25 30 Pro Gln Phe Trp Ile Asn Ile Leu Leu Thr Leu Leu Gly Tyr Ile Pro 35 40 45 Gly Ile Val His Ala Val Trp Val Ile Ala Lys Lys 50 55 60 <210> 44 <211> 114 <212> PRT <213> Cyanobacterium ATCC51142 <400> 44 Leu Ile Met Ser Tyr Ser Leu Ser Pro Gln Glu Lys Ser Asn Thr Thr 1 5 10 15 Leu Phe Ser Leu Cys Asn Gln Arg Lys His Gln Arg Tyr Tyr Asn Ser 20 25 30 Gln Gly Ser Ser Val Glu Leu Leu Ile Asp Gly Gln Glu Ile Lys Gln 35 40 45 Ser Leu Lys Gly Met Ile Ile Asp Asp Ser Phe Ser Gly Cys Gly Leu 50 55 60 Ile Ile Ile Gly Glu Glu Lys Leu His Ile Gly Gln Leu Cys Arg Leu 65 70 75 80 Arg Ile Lys Gly Ile Asp Ser Ile Leu Cys Gln Ile Ile Trp Leu Arg 85 90 95 Arg Leu Glu Lys Ser Ile Thr Arg Ile Gly Val Lys Tyr Leu Ile Lys 100 105 110 Ser Glu <210> 45 <211> 233 <212> PRT <213> Cyanobacterium ATCC51142 <400> 45 Met Val Pro Leu Asn Asp Asn Asn Pro Thr Gln Arg Thr Ala Tyr Val 1 5 10 15 Thr Tyr Gly Leu Ile Ile Leu Asn Val Leu Val Phe Phe Lys Glu Leu 20 25 30 Ser Leu Ser Ser Glu Glu Leu Ser Gln Phe Phe Gln Tyr Tyr Ala Ile 35 40 45 Val Pro Lys Gln Leu Thr Ala Gly Phe Ser Gly Val Asp Phe Tyr His 50 55 60 Pro Ile Pro Glu Trp Met Thr Leu Phe Thr Ser Gln Phe Leu His Ala 65 70 75 80 Gly Phe Leu His Ile Ala Gly Asn Met Leu Phe Leu Trp Ile Phe Gly 85 90 95 Asn Asn Val Glu Asp Lys Leu Gly His Val Lys Tyr Leu Ile Phe Tyr 100 105 110 Leu Thr Cys Gly Val Leu Ala Gly Leu Ser Gln Trp Tyr Phe Ala Pro 115 120 125 Tyr Ser Glu Val Pro Ser Leu Gly Ala Ser Gly Ala Ile Ala Gly Val 130 135 140 Met Gly Ala Tyr Ile Leu Arg Phe Pro Gln Ala Gln Val Leu Thr Leu 145 150 155 160 Ile Pro Leu Gly Ile Phe Ile Thr Thr Ile Arg Ile Pro Ala Val Phe 165 170 175 Phe Leu Gly Phe Trp Phe Leu Gln Gln Ala Ile Tyr Gly Phe Ala Ser 180 185 190 Leu Asn Val Gln Thr Asn Val Gly Met Glu Gly Gly Gly Val Ala Tyr 195 200 205 Trp Ala His Ala Gly Gly Phe Val Phe Gly Phe Ile Leu Gly Pro Leu 210 215 220 Leu Gly Leu Leu Asp Asp Lys Asn Arg 225 230 <210> 46 <211> 161 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 46 Met Val Lys Arg Phe Ile Leu Met Ala Ile Ala Ala Ile Phe Phe Phe 1 5 10 15 Leu Gln Phe Pro Ile His Asn Ala Asn Ala Leu Glu Leu Thr Glu Glu 20 25 30 Thr Arg Thr Val Pro Leu Asn Glu Glu Gly Glu Thr Ile Thr Leu Thr 35 40 45 Ser Glu Gln Ile Thr Asn Gly Gln Arg Leu Phe Ile Arg Glu Cys Thr 50 55 60 Gln Cys His Leu Gln Gly Lys Thr Lys Thr Asn Asn Asn Val Ser Leu 65 70 75 80 Gly Leu Glu Asp Leu Ser Leu Ala Glu Pro Pro Arg Asp Asn Val Leu 85 90 95 Gly Leu Val Asp Tyr Leu Lys Tyr Pro Thr Ser Tyr Asp Gly Glu Asp 100 105 110 Asp Tyr Thr Glu Leu His Val Asn Val Ser Arg Pro Asp Ile Tyr Pro 115 120 125 Glu Leu Arg Asp Phe Thr Glu Asp Asp Leu Tyr Asp Val Ser Gly Tyr 130 135 140 Ile Leu Val Ala Pro Lys Leu Asp Ser Tyr Trp Gly Gly Ser Ile Tyr 145 150 155 160 Phe <210> 47 <211> 31 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 47 Leu Leu Arg Gly Glu Asn Arg Phe Leu Ser Leu Lys Lys Ser Pro Ile 1 5 10 15 Pro Pro Arg Glu Gly Lys Phe Lys Asp Gln Glu Leu Trp Tyr Arg 20 25 30 <210> 48 <211> 237 <212> PRT <213> Blueberry ATCC51142 <400> 48 Met Ser Ala Thr Cys Val Ile Asn Gly Trp Glu Arg Pro Glu Lys Thr 1 5 10 15 Pro Met Leu Lys Leu Tyr His Leu Pro Ile Ser Phe Asn Ser Arg Arg 20 25 30 Val Trp Ile Ala Leu Leu Glu Lys Gly Leu Ser Phe Glu Leu Ile Pro 35 40 45 Met Lys Leu Asn Gly Asp Gln Leu Thr Pro Glu Phe Leu Ala Leu Asn 50 55 60 Pro Phe His His Ile Pro Val Leu Val Asp Glu Ala Phe Ser Leu Phe 65 70 75 80 Glu Ser Leu Ala Ile Leu Asp Tyr Leu Glu Ala Lys Tyr Pro Thr Pro 85 90 95 Ser Leu Val Pro Ser Asp Pro Gln Gly Leu Gly Thr Val Lys Met Ile 100 105 110 Asn Leu Val Thr Leu Asn Glu Leu Leu Pro Ala Thr Thr Pro Leu Ile 115 120 125 Gln His Ser Met Gly Phe Ile Thr Leu Asp Glu Gln Arg Ile Ala Asn 130 135 140 Thr Lys Glu Lys Val Ala Val Val Leu Asn Phe Phe Glu Thr Ser Leu 145 150 155 160 Gly Asp Arg Ser Tyr Ile Val Gly Asn Thr Leu Thr Leu Ala Asp Ile 165 170 175 Val Ala Gly Thr Met Val Gly Phe Leu Pro Gln Met Gly Val Ser Leu 180 185 190 Ser Ala Tyr Pro Gln Leu Thr Ala Trp Thr Lys Gln Leu Ser Gln Arg 195 200 205 Glu Ser Trp Gln Gln Thr Glu Pro Gln Pro Glu Glu Ile Asp Lys Phe 210 215 220 Arg Glu Thr Met Lys Lys Leu Met Ala Gln Arg Gly Ser 225 230 235 <210> 49 <211> 180 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 49 Met Thr Gln Val Ser Gly Ser Ser Asp Val Pro Asp Met Gly Arg Arg 1 5 10 15 Gln Phe Met Asn Leu Leu Thr Phe Gly Thr Ile Thr Gly Val Ala Ala 20 25 30 Gly Ala Leu Tyr Pro Val Val Lys Tyr Phe Ile Pro Pro Ser Ser Gly 35 40 45 Gly Ala Gly Gly Gly Ile Thr Ala Lys Asp Ala Leu Gly Asn Asp Ile 50 55 60 Met Ala Ser Asn Phe Leu Ala Thr His Asn Gly Gly Asp Arg Val Leu 65 70 75 80 Ala Gln Gly Leu Lys Gly Asp Pro Thr Tyr Leu Val Val Glu Gly Glu 85 90 95 Asn Ala Ile Ala Asp Tyr Gly Ile Asn Ala Val Cys Thr His Leu Gly 100 105 110 Cys Val Val Pro Trp Asn Ala Ser Glu Asp Lys Phe Ile Cys Pro Cys 115 120 125 His Gly Ser Gln Tyr Asn Ala Gln Gly Lys Val Val Arg Gly Pro Ala 130 135 140 Pro Leu Ser Leu Ala Leu Ala His Val Asn Val Ser Glu Asp Asp Lys 145 150 155 160 Val Val Phe Ser Glu Trp Thr Glu Thr Asp Phe Arg Thr Glu Gln Asp 165 170 175 Pro Trp Trp Ala 180 <210> 50 <211> 303 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 50 Leu Ile Val Ala Ala Thr Val Ala Leu Phe Ile Gly Asn Val Gln Ser 1 5 10 15 Ala Asn Ala Tyr Pro Phe Trp Ala Gln Glu Thr Ala Pro Glu Thr Pro 20 25 30 Arg Glu Ala Thr Gly Arg Ile Val Cys Ala Asn Cys His Leu Ala Glu 35 40 45 Lys Ala Ala Glu Val Glu Ile Pro Gln Ser Val Leu Pro Asp Thr Val 50 55 60 Phe Lys Ala Val Val Lys Ile Pro Tyr Asp Leu Asp Ser Gln Gln Val 65 70 75 80 Leu Gly Asp Gly Ser Lys Gly Gly Leu Asn Val Gly Ala Val Leu Met 85 90 95 Leu Pro Glu Gly Phe Lys Ile Ala Pro Asp Glu Arg Ile Pro Glu Glu 100 105 110 Ile Lys Glu Glu Met Gly Ser Val Tyr Phe Gln Ser Tyr Ser Glu Gly 115 120 125 Gln Asp Asn Val Val Leu Val Gly Pro Leu Pro Gly Glu Gln Tyr Gln 130 135 140 Glu Ile Ile Phe Pro Ile Leu Ser Pro Asp Pro Ser Lys Asp Lys Asn 145 150 155 160 Val Asn Phe Gly Lys Tyr Gln Val His Leu Gly Ala Asn Arg Gly Arg 165 170 175 Gly Gln Ile Tyr Pro Thr Gly Gln Pro Ser Asn Asn Asn Val Phe Lys 180 185 190 Ala Ser Asn Ala Gly Thr Ile Ser Lys Ile Thr Asp Gln Glu Asp Gly 195 200 205 Ser Tyr Ile Val Thr Ile Ala Thr Ala Glu Gly Asp Val Asp Glu Thr 210 215 220 Ile Pro Ala Gly Pro Gln Leu Met Val Ser Glu Gly Met Glu Val Glu 225 230 235 240 Ala Gly Gln Ala Leu Thr Asn Asn Pro Asn Val Gly Gly Phe Gly Gln 245 250 255 Lys Asp Thr Glu Val Val Leu Gln Ser Pro Gly Arg Ile Lys Gly Leu 260 265 270 Ile Leu Phe Leu Ala Gly Ile Met Leu Ala Gln Ile Leu Leu Val Ile 275 280 285 Lys Lys Lys Gln Val Glu Arg Val Gln Ala Ala Glu Met Asn Phe 290 295 300 <210> 51 <211> 32 <212> PRT <213> Cyanobacterium ATCC51142 <400> 51 Val Ile Met Ile Leu Ile Val Val Val Ala Leu Ile Tyr Glu Ile Phe 1 5 10 15 Ser Glu Val Val Ile Asn Ala Pro Phe Asp Tyr Asn Pro Arg Asp Glu 20 25 30 <210> 52 <211> 310 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 52 Leu Thr Thr Ile Leu Gly Met Asn Ser Ile Thr Gly Cys Ser Ser Ser 1 5 10 15 Asn Pro Val Asp Ser Gly Ile Asn Lys Ser Asp Asn Gln Glu Gln Ser 20 25 30 Asn Ser Gln Asp Gln Leu Asp Ile Thr Val Ser Ile Ile Pro Gln Lys 35 40 45 Tyr Phe Val Lys Lys Ile Gly Gly Asp Leu Val Arg Val Asn Val Met 50 55 60 Val Glu Gln Gly Val Leu Pro His Thr Tyr Glu Pro Lys Pro Gln Gln 65 70 75 80 Leu Gln Ala Leu Ser Glu Ala Glu Ala Tyr Ile Gly Ile Gly Ile Pro 85 90 95 Phe Glu Thr Ala Trp Met Asp Arg Ile Lys Glu Ala Asn Pro Lys Met 100 105 110 Leu Met Val Asp Ser Thr Gln Gly Ile Asp Arg Leu Thr Met Ile Ala 115 120 125 His Asp His His Glu Glu Glu Asp His Gly His His Glu Glu Glu Thr 130 135 140 Thr Leu Asp Pro His Val Trp Leu Ser Pro Arg Leu Val Lys Ile Gln 145 150 155 160 Ala Gln Lys Ile Tyr Glu Thr Leu Val Lys Leu Asp Pro Lys His Gln 165 170 175 Glu Thr Tyr Gln Thr Asn Leu Asn Ser Phe Leu Gln Glu Ile Glu Glu 180 185 190 Leu Asp His Gln Val Arg Asn Asn Leu Ala Asn Leu Lys Gln Arg Lys 195 200 205 Phe Ile Val Phe His Pro Ala Trp Gly Tyr Phe Ala Glu Glu Tyr Asn 210 215 220 Leu Thr Gln Val Pro Ile Glu Val Gly Gly Gln Glu Pro Ser Ala Ser 225 230 235 240 Glu Leu Gly Asp Leu Ile Lys Glu Ala Lys Lys Glu Asn Ile Lys Val 245 250 255 Ile Phe Ala Gln Pro Glu Leu Ser Ser Gln Ala Ala Lys Thr Ile Ala 260 265 270 Lys Glu Ile Asn Gly Glu Val Leu Leu Ile Ser Pro Thr Ala Ala Asp 275 280 285 Trp Ser Asn Asn Leu Leu Glu Val Ser Gln Thr Phe Ala Lys Val Leu 290 295 300 Lys Glu Glu Asn Asn Gln 305 310 <210> 53 <211> 259 <212> PRT <213> Cyanobacterium ATCC51142 <400> 53 Met Lys Thr Glu Val Ile Asn Leu Ser His Val Trp Ala Lys Tyr Asn 1 5 10 15 His Arg Asn Pro Ile Leu Glu Asp Ile Asn Leu Thr Ile Tyr Glu Gly 20 25 30 Asp Phe Val Gly Leu Ile Gly Pro Asn Gly Gly Gly Lys Thr Thr Leu 35 40 45 Phe Lys Val Leu Leu Gly Leu Ile Lys Pro Tyr Gln Gly Thr Val Lys 50 55 60 Ile Leu Gly Asn Thr Val Ser Lys Gly Arg Arg Tyr Ile Gly Tyr Val 65 70 75 80 Pro Gln Leu Val Glu Leu Asp Arg Glu Phe Pro Val Arg Val Ala Asp 85 90 95 Val Val Arg Met Gly Arg Leu Gly Lys Arg Arg Leu Leu Gln Arg Tyr 100 105 110 Thr Pro Gln Asp Glu Ile Ile Val Asn Arg Thr Leu Glu Gln Val Gly 115 120 125 Met Ile Glu Leu Arg Asn Arg Pro Ile Ala Glu Leu Ser Gly Gly Gln 130 135 140 Arg Gln Arg Val Tyr Ile Ala Arg Ala Leu Ala Ser Glu Pro Arg Ile 145 150 155 160 Leu Leu Leu Asp Glu Pro Thr Ala Ser Val Asp Pro Gln Arg Gln Thr 165 170 175 Ser Ile Tyr Glu Leu Leu Lys Glu Leu Asn Gln Leu Ile Thr Ile Val 180 185 190 Met Ile Ser His Asp Ile Gly Ala Ile Ser Ala Tyr Val Lys Thr Val 195 200 205 Gly Cys Leu Asn His Arg Leu Phe Phe His Gly Asp Pro Pro Leu Ser 210 215 220 Thr Glu Thr Ile Glu Gln Thr Tyr Gln Cys Pro Val Asp Leu Ile Ala 225 230 235 240 His Gly Val Pro His Arg Val Leu Ser Asn His Asp Cys Pro Leu His 245 250 255 Tyr His Glu <210> 54 <211> 271 <212> PRT <213> Cyanobacterium ATCC51142 <400> 54 Met Leu Glu Thr Leu Trp Glu Ser Leu Gln Phe Asp Phe Met Arg Asn 1 5 10 15 Ala Leu Phe Ala Gly Ile Leu Val Ser Ile Ala Cys Gly Ile Ile Gly 20 25 30 Thr Phe Val Val Ile Asn Arg Ile Val Phe Ile Ser Gly Gly Ile Ala 35 40 45 His Ala Ala Tyr Gly Gly Ile Gly Leu Gly Tyr Phe Phe Lys Ile Asn 50 55 60 Pro Ile Phe Gly Ala Ile Phe Phe Ala Leu Leu Ser Ala Leu Gly Met 65 70 75 80 Gly Leu Val Val Arg Lys Thr Glu Gln Arg Ala Asp Ser Leu Ile Gly 85 90 95 Val Met Trp Ala Val Gly Met Ala Ile Gly Ile Ile Leu Ile Asp Leu 100 105 110 Thr Pro Gly Tyr Lys Ala Asp Leu Met Ser Tyr Leu Phe Gly Ser Ile 115 120 125 Leu Thr Val Ser Gln Glu Asn Leu Met Ile Met Leu Val Leu Asp Val 130 135 140 Ile Ile Val Leu Val Val Ser Leu Phe Tyr Lys Glu Phe Leu Ala Ile 145 150 155 160 Ser Phe Asp Pro Thr Phe Ala Met Thr Arg Asn Val Pro Val Asp Ser 165 170 175 Leu Tyr Leu Leu Leu Val Gly Ala Ile Ala Leu Thr Val Val Met Val 180 185 190 Met Gln Val Val Gly Leu Ile Leu Val Ile Ala Leu Leu Thr Ile Pro 195 200 205 Ala Ala Ile Ala Gly Gln Phe Leu Lys Asp Ile Lys Tyr Ile Met Leu 210 215 220 Val Ser Ile Val Leu Gly Met Leu Phe Thr Thr Val Gly Leu Met Ile 225 230 235 240 Ser Tyr Phe Phe Asn Val Thr Ser Gly Ala Thr Ile Ile Leu Val Ser 245 250 255 Gly Thr Ala Tyr Leu Ile Ser Leu Gly Val Lys Thr Leu Gln Ile 260 265 270 <210> 55 <211> 42 <212> PRT <213> Blueberry ATCC51142 <400> 55 Leu Cys Tyr Ser Ser Pro Glu Gly Ile Lys Gln Gly Arg Gln Glu Gly 1 5 10 15 Lys Leu Ala Ala Lys Ile Ala Ser Ile Pro Arg Leu Val Thr Leu Glu 20 25 30 Leu Ser Val Glu Gln Ile Ala Gln Ala Leu 35 40 <210> 56 <211> 88 <212> PRT <213> Blueberry ATCC51142 <400> 56 Leu Pro Leu Ile Ser Arg Gln Glu Ile Glu Lys Met Phe Ser Leu Ser 1 5 10 15 Asp Leu Arg Glu Thr Lys Val Tyr Gln Glu Ala Leu Glu Glu Gly Ile 20 25 30 Glu Lys Gly Ile Glu Gln Gly Ile Glu Gln Gly Arg Gln Glu Gly Glu 35 40 45 Leu Ala Ala Lys Ile Ala Ser Ile Pro Arg Leu Val Ala Leu Gly Leu 50 55 60 Thr Ile Glu Gln Ile Ala Gln Ala Leu Glu Leu Asp Ile Glu Gln Val 65 70 75 80 Ser Asn Ile Val Glu Ser Asn Lys 85 <210> 57 <211> 354 <212> PRT <213> Cyanobacterium ATCC51142 <400> 57 Met Thr Gly Ala Asn Asp Thr Pro Tyr Leu Leu Arg Ala Ala Arg Gly 1 5 10 15 Glu Ile Leu Asp Arg Pro Pro Val Trp Met Met Arg Gln Ala Gly Arg 20 25 30 Tyr Met Lys Val Tyr Arg Asp Leu Arg Asp Lys Tyr Pro Ser Phe Arg 35 40 45 Glu Arg Ser Glu Asn Pro Asp Leu Ala Ile Glu Ile Ser Leu Gln Pro 50 55 60 Trp Arg Ala Phe Gln Pro Asp Gly Val Ile Met Phe Ser Asp Ile Leu 65 70 75 80 Thr Pro Leu Pro Gly Met Gly Ile Pro Phe Asp Ile Val Glu Ser Lys 85 90 95 Gly Pro Val Ile Asp Pro Pro Ile Arg Thr Lys Glu Gln Val Asp Asn 100 105 110 Leu Arg Pro Leu Asp Pro Glu Glu Ser Leu Pro Phe Ile Lys Thr Ile 115 120 125 Leu Gln Ser Leu Arg Gln Glu Val Gly Asn Gln Ser Thr Val Leu Gly 130 135 140 Phe Val Gly Ser Pro Trp Thr Leu Ala Ala Tyr Ala Ile Glu Gly Lys 145 150 155 160 Ser Ser Lys Asn Tyr Ala Ile Ile Lys Ser Met Ala Phe Ser Gln Pro 165 170 175 Glu Ile Leu His Ser Phe Leu Ser Lys Ile Ala Asp Ala Ile Ala Ile 180 185 190 Tyr Val Arg Tyr Gln Ile Asp Cys Gly Ala Gln Val Val Gln Leu Phe 195 200 205 Asp Ser Trp Ala Gly Gln Leu Ser Pro Gln Asp Tyr Glu Thr Phe Ala 210 215 220 Leu Pro Tyr Gln Gln Gln Val Val Arg Gln Val Lys Glu Thr His Pro 225 230 235 240 Asp Thr Pro Leu Ile Leu Tyr Ile Ser Gly Ser Ala Gly Val Leu Glu 245 250 255 Arg Met Gly Gln Ser Gly Val Asp Ile Val Ser Val Asp Trp Thr Val 260 265 270 Asp Met Ala Glu Ala Arg Gln Arg Leu Gly Arg Asp Met Lys Val Gln 275 280 285 Gly Asn Ile Asp Pro Gly Val Leu Phe Gly Ser Gln Asp Phe Ile Lys 290 295 300 Ala Arg Ile Leu Asp Thr Val Arg Lys Ala Gly Arg Gly Gly His Ile 305 310 315 320 Leu Asn Leu Gly His Gly Val Leu Val Gly Thr Pro Glu Asp Asn Val 325 330 335 Arg Cys Phe Phe Glu Thr Ala Lys Gln Val Asp Gln Leu Leu Ala Val 340 345 350 Pro Val <210> 58 <211> 368 <212> PRT <213> Cyanobacterium ATCC51142 <400> 58 Leu Ile Gln Lys Asn Pro Phe Lys Gln Ala Met Val Asn Thr Leu Gln 1 5 10 15 Lys Pro Glu Phe Glu Glu Leu Arg Pro Gly Ile Lys Val Pro Ala Lys 20 25 30 Glu Thr Ile Leu Thr Pro Arg Phe Tyr Thr Thr Asp Phe Glu Ala Met 35 40 45 Ala Lys Met Asp Ile Ser Val Asn Glu Glu Glu Leu Gln Ala Ile Leu 50 55 60 Glu Glu Phe Arg Thr Asp Tyr Asn Arg His His Phe Ile Arg Arg Glu 65 70 75 80 Glu Phe Ala Gln Thr Trp Asp His Ile Asp Gly Asp Thr Arg Arg Leu 85 90 95 Phe Val Glu Phe Leu Glu Arg Ser Cys Thr Ala Glu Phe Ser Gly Phe 100 105 110 Leu Leu Tyr Lys Glu Leu Gly Arg Arg Leu Lys Asp Lys Ser Pro Val 115 120 125 Leu Ala Glu Cys Phe Thr Leu Met Ser Arg Asp Glu Ala Arg His Ala 130 135 140 Gly Phe Leu Asn Lys Ala Leu Ser Asp Phe Asn Met Ser Leu Asp Leu 145 150 155 160 Gly Phe Leu Thr Lys Ser Arg Ser Tyr Thr Phe Phe Gln Pro Lys Phe 165 170 175 Ile Phe Tyr Ala Thr Tyr Leu Ser Glu Lys Ile Gly Tyr Trp Arg Tyr 180 185 190 Ile Thr Ile Tyr Arg His Leu Glu Gln His Pro Glu Asp Arg Ile Tyr 195 200 205 Pro Ile Phe Asn Phe Phe Glu Asn Trp Cys Gln Asp Glu Asn Arg His 210 215 220 Gly Asp Phe Phe Asp Ala Ile Met Lys Ala Thr Pro Asp Ile Leu Asn 225 230 235 240 Asp Trp Lys Ala Arg Leu Trp Cys Arg Phe Phe Leu Leu Ser Val Phe 245 250 255 Only Thr Met Tyr Leu Asn Asp Ile Gln Arg Ser Asp Phe Tyr Ala Ser 260 265 270 With Gly Leu Asp Ala Arg Asp Tyr Asp with Tyr Val with Glu Lys Thr 275 280 285 Asn Glu Thr Ala Gly Arg Val Phe Pro Ile Val Leu Asp Val Asp Asn 290,295,300 Pro Glu Phe Tyr Asp Ser Leu Glu Val Cys Val Lys Asn Asn Glu Lys 305 310 315 320 Leu Arg Glu Ile Asp Ala Ser Asn Ser Pro Thr Pro Val Lys Phe Leu 325 330 335 Arg Lys Leu Pro Ala Phe Ile Ser Asn Gly Val Gln Phe Leu Lys Leu 340 345 350 Tyr Phe Met Lys Pro Ile Arg Val Asp His Leu Glu Gly Thr Val Arg 355 360 365 <210> 59 <211> 147 <212> PRT <213> ATCC51142 <400> 59 Met Ile Lys Leu Will Be Asn Will Be Lys Leu Will Be Thr Leu Will Be Leu 1 5 10 15 Phe Thr Ile Leu Ile Asn Leu Leu Leu Ile Ser Cys Ala Glu Thr Lys 20 25 30 Val Ser Gln Cys Gln Lys Ile Ile Leu Leu Thr Gln Lys Met Ala Glu 35 40 45 Glu Ser Glu Ser Tyr Arg Gln Thr Thr Asp Ile Lys Lys Val Leu Gln 50 55 60 Val Ala Asp Leu Phe Glu Glu Thr Ser Gln Gln Met Lys Gln Leu Lys 65 70 75 80 Ile Glu Asp Glu Gln Leu Gln Glu Tyr Gln Met Gly Phe Ala Asp Ile 85 90 95 Tyr Gln Gly Asn Ala Asp Thr Thr Arg Gln Phe Val Ala Ala Leu Lys 100 105 110 Asp Lys Asp Ile Asp Thr Ala Lys Leu Met Gln Gln Gln Val Gln Gln 115 120 125 Leu Gly Lys Lys Glu Gln Asp Leu Gly Thr Glu Met Asn Ala Tyr Cys 130 135 140 Gln Pro Asp 145 <210> 60 <211> 94 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 60 Met Val Gln Asn Thr Gln Lys Ile Glu Thr Ile Leu Glu Arg Met Gly 1 5 10 15 Glu Thr Val Ile Ala Thr Thr Glu Thr Val Glu Ser Leu Ala Glu Lys 20 25 30 Met Glu Asn Leu Val Glu Gln Ile Gln Gln Gln Glu Gln Gln Ile Gln 35 40 45 Gln Gln Gly Tyr Gln Ile Phe Ala Leu Ser Glu Ser Ile Gln Thr Leu 50 55 60 Val Asp Ala Gln Thr Glu Ser Arg Glu Gln Leu Thr Gln Leu Thr Tyr 65 70 75 80 Leu Ile His Thr Leu Val His Ser Leu Thr Asn Gln Glu Glu 85 90 <210> 61 <211> 336 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 61 Val Leu Val Ser Asp Thr Lys Pro Thr Ile Leu Val Thr Gly Gly Ala 1 5 10 15 Gly Tyr Ile Gly Ser His Ala Val Leu Ser Leu Gln Lys Ala Gly Tyr 20 25 30 His Val Ile Val Phe Asp Asn Leu Ser Tyr Gly His Pro Glu Ile Ile 35 40 45 Lys Asp Val Leu Gln Val Glu Leu Ile Val Gly Asp Thr Gln Asn Arg 50 55 60 Thr Leu Leu Asp Glu Leu Phe Ser Thr Arg Asn Ile Ala Ala Val Met 65 70 75 80 His Phe Ala Ala Phe Ile Ala Val Gly Glu Ser Val Gln Ala Pro Ala 85 90 95 Ile Tyr Tyr Gln Asn Asn Val Val Gly Thr Leu Thr Leu Leu Glu Ala 100 105 110 Met Met Ala Ala Asn Ile Asn Lys Phe Val Phe Ser Ser Thr Cys Ala 115 120 125 Ile Tyr Gly Met Pro Gln Glu Ile Pro Met Thr Glu Gln His Pro Asn 130 135 140 His Pro Leu Ser Pro Tyr Ala Ser Ser Lys Tyr Met Val Glu Lys Ile 145 150 155 160 Leu Lys Asp Phe Asp Gln Ala Tyr Gly Leu Lys Ser Val Ile Phe Arg 165 170 175 Tyr Phe Asn Ala Ser Gly Ala Asp Pro Ser Gly Asn Leu Gly Glu Asp 180 185 190 His Thr Pro Glu Thr His Leu Ile Pro Leu Ala Leu Leu Thr Ala Leu 195 200 205 Lys Lys Arg Asp His Leu Phe Ile Phe Gly Thr Asp Tyr Asp Thr His 210 215 220 Asp Gly Thr Ala Ile Arg Asp Tyr Ile His Val Asn Asp Leu Ala Ser 225 230 235 240 Ala His Ile Leu Gly Leu Glu Tyr Leu Leu Asn Gly Gly Glu Ser Glu 245 250 255 Met Phe Asn Leu Gly Asn Gly Asn Gly Phe Ser Val Lys Glu Val Ile 260 265 270 Asp Met Ala Lys Lys Val Thr Gly Ile Asp Phe Leu Val Lys Glu Ser 275 280 285 Asp Arg Arg Pro Gly Asp Val Pro Ile Leu Val Gly Ser Ser Gln Lys 290 295 300 Ala Gln Ser Val Leu Gly Trp Gln Pro Gln Tyr Ser Asp Leu Gln Thr 305 310 315 320 Ile Val Asn His Ala Trp Gln Trp His Gln Lys Arg His Gly Asn Ala 325 330 335 <210> 62 <211> 519 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 62 Leu Val Tyr Ser Leu Tyr Thr Glu Lys Ser Ile His Val Phe Tyr Asn 1 5 10 15 Thr Tyr Tyr Tyr Leu Thr Met Leu Asp Thr Leu Asp Leu Lys Leu Thr 20 25 30 Leu Asp Lys Glu Thr Tyr Lys Thr Glu Ile Glu Ala Leu Met Arg Gln 35 40 45 Leu Arg Ser Leu Gln Lys Asp Cys Trp Asp Asn Gln Leu Pro Val Ile 50 55 60 Ile Val Leu Glu Gly Trp Ala Ala Ala Gly Lys Gly Lys Leu Leu Gln 65 70 75 80 Lys Thr Ile Gly Tyr Met Asp Pro Arg Gly Phe Asn Val Tyr Pro Ile 85 90 95 Leu Ala Ala Thr Glu Gln Glu Lys Lys Tyr Pro Phe Leu Trp Arg Phe 100 105 110 Trp His Asn Leu Pro Pro Lys Gly Ser Ile Gly Ile Phe Tyr His Ser 115 120 125 Trp Tyr Thr His Val Leu Glu Asp Arg Leu Phe Gly Ile Glu Thr Asp 130 135 140 Gly Ser Ile Pro Leu Leu Met Arg Asp Ile Asn Ala Phe Glu Arg Gln 145 150 155 160 Leu Val Asp Asp Gly Val Ala Ile Ala Lys Phe Trp Ile His Leu Ser 165 170 175 Gln Lys Glu Leu Lys Lys Arg Leu Lys Thr Tyr Ala Ser Asp Glu Leu 180 185 190 Glu Ser Trp Arg Val Arg Pro Glu Asp Trp Gln Gln Ala Lys Glu Tyr 195 200 205 Asp Arg Tyr Gly Thr Phe Ala Glu Glu Met Leu Thr Tyr Thr Ser Thr 210 215 220 Gly His Ala Pro Trp Thr Leu Val Glu Gly Asp Cys Lys Arg Trp Ala 225 230 235 240 Arg Val Lys Val Leu Ser Gln Ile Val Ala Val Ile Thr Gln Ala Leu 245 250 255 Asp Arg Leu Arg Leu Pro Lys Thr Asp Ile Pro Ser Leu Pro Pro Gln 260 265 270 Thr Glu Leu Gln Pro Thr Glu Pro Asp Phe Leu Gly Lys Ile Asp Leu 275 280 285 Gly Leu His Leu Phe Lys Asp Asp Tyr Lys Gln Arg Leu Arg Glu Ala 290 295 300 Gln Val Lys Leu Arg Glu Leu Gln Leu Gln Ile Phe Lys Lys Lys Val 305 310 315 320 Pro Val Leu Val Leu Phe Glu Gly Trp Asp Ala Ala Gly Lys Gly Gly 325 330 335 Ala Ile Lys Arg Leu Thr Asp Thr Leu Asp Pro Arg Ser Tyr Lys Val 340 345 350 His Ala Phe Ser Ala Pro Thr Ser Glu Glu Leu Asn Tyr His Tyr Leu 355 360 365 Trp Arg Phe Trp Arg Gln Ile Pro Ala Gln Gly Ser Ile Gly Ile Phe 370 375 380 Asp Arg Ser Trp Tyr Gly Arg Val Leu Val Glu Arg Val Glu Gly Phe 385 390 395 400 Ala Ser Glu Leu Glu Trp Arg Arg Ser Tyr Lys Glu Ile Asn Glu Phe 405 410 415 Glu Ser Gln Leu Thr Ala Ser Gly Tyr Val Ile Val Lys Phe Trp Leu 420 425 430 His Ile Ser Phe Glu Glu Gln Leu Lys Arg Phe Glu Asp Arg Lys Asn 435 440 445 Asn Pro Phe Lys Ser Tyr Lys Leu Thr Asp Glu Asp Trp Arg Asn Arg 450 455 460 Glu Lys Trp Pro Leu Tyr Tyr Val Ala Val Asn Gln Met Ile Ala Arg 465 470 475 480 Thr Ser Thr Pro Tyr Ala Pro Trp Thr Ile Val Pro Gly Asn Asp Lys 485 490 495 Tyr Tyr Ala Arg Val His Val Leu Glu Thr Val Ile His Ala Ile Glu 500 505 510 Thr Glu Leu Lys Gln Arg Asp 515 <210> 63 <211> 128 <212> PRT <213> Cyanobacterium ATCC51142 <400> 63 Val Glu Pro Lys Thr Gly Lys Thr Leu Trp Tyr Leu Ile Pro Arg Val 1 5 10 15 Asn Asn Lys Trp Leu Ser Leu Val Tyr Glu Ala Phe Ala Glu Asp Val 20 25 30 Gly Leu Asn Lys Asp Lys Ile Ile Phe Leu Val Glu Asp Asn Ala Gly 35 40 45 Trp His Arg Ser Gln Lys Leu Lys Ile Pro Asn Gly Ile Ile Val Glu 50 55 60 Phe Leu Pro Ala Tyr Ser Pro Glu Leu Gln Pro Ala Glu Arg Leu Trp 65 70 75 80 Thr Leu Val Asp Glu Pro Leu Val Asn Glu Tyr Phe Glu Thr Ile Glu 85 90 95 Glu Ile Glu Asp Ile Leu Ala Ile Arg Cys Cys Leu Leu Gln Asn Met 100 105 110 Thr Glu Glu Ile Lys Asn Leu Thr Asn Tyr His Trp Leu Lys Tyr Ser 115 120 125 <210> 64 <211> 406 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 64 Met Ala Lys Gly Phe Ala Asp Gln Lys Gln Ser Ser Lys Lys Lys Lys 1 5 10 15 Glu Lys Gly Asn Ile Ala Ser Ile Asp Glu Ile Gln Asn Asn Leu Leu 20 25 30 Gly Tyr Val Lys Glu Ile Glu Asp Pro Arg Val Gln Arg Ser Lys Lys 35 40 45 His Leu Leu Lys Asp Val Leu Ala Ile Ala Ile Leu Ala Val Ile Ala 50 55 60 Gly Ser Gln Gly Trp Glu Asp Met Glu Asn Tyr Gly Ile Ala Lys Gln 65 70 75 80 Glu Trp Leu Ser Glu Phe Leu Glu Leu Pro His Gly Ile Pro Ser Asp 85 90 95 Asp Thr Phe Arg Arg Val Phe Glu Arg Ile Asp Pro Glu Ser Leu Gln 100 105 110 Lys Cys Leu Gln Lys Trp Val Gln Ser Ile Met Asn Ser Ile Gln Gly 115 120 125 Glu Ile Ile Pro Ile Asp Gly Lys Thr Leu Arg Gly Ser Tyr Asp Arg 130 135 140 Asn Ala Gly Gln Cys Ala Leu His Thr Val Thr Ala Trp Ala Ser Gln 145 150 155 160 Gln Ser Leu Val Leu Gly Gln Val Lys Val Glu Asn Tyr Ser Asn Glu 165 170 175 Ile Thr Ala Ile Pro Ala Leu Leu Glu Leu Leu Asp Ile Thr Gly Ser 180 185 190 Ile Ile Thr Ile Asp Ala Met Gly Thr Gln Thr Ser Ile Ile Gln Gln 195 200 205 Ile Cys Arg Gln Lys Ala Asp Tyr Ile Val Thr Leu Lys Ala Asn His 210 215 220 Pro Thr Leu Phe Ser Gln Val Lys Gln Trp Phe Thr Asp Thr Gln Asn 225 230 235 240 Asn Gly Trp Asp Gly Ile Glu His Asp Tyr Tyr Lys Ser Val Thr Lys 245 250 255 Gly His His Arg Thr Glu Lys Arg Tyr Val Trp Ala Ile Pro Val Ala 260 265 270 Ala Met Gly Glu Leu Tyr Gln Gln Gln Gln Trp His Gly Leu Gln Thr 275 280 285 Ile Val Val Val Glu Arg Ile Arg His Leu Trp Asn Lys Thr Thr His 290 295 300 Asp Ile Gln Phe Tyr Leu Thr Ser Leu Pro Pro Asn Ala Gln Phe Leu 305 310 315 320 Cys His Ala Ile Arg Thr His Trp Ser Ile Glu Asn Asn Leu His Trp 325 330 335 Thr Leu Asp Val Thr Phe Ser Glu Asp Gln Cys Arg Ile Arg Ser Glu 340 345 350 Tyr Ser Pro Gln Asn Phe Ala Leu Leu Arg Arg Leu Ala Leu Asn Val 355 360 365 Leu His Gln Glu Lys Thr Phe Lys Arg Ser Leu Arg Gln Lys Met Lys 370 375 380 Gln Ala Ala Met Asn Asn Asn Tyr Met Met Thr Val Leu Asn Ser Phe 385 390 395 400 Cys Gln Ala Asp Phe Arg 405 <210> 65 <211> 67 <212> PRT <213> Synechococcus sp. ATCC51142 <400> 65 Met Thr Thr Ile Asn Tyr Thr Pro Asp Pro Glu Lys Lys Ala Gln Leu 1 5 10 15 Ser Gln Glu Gln Leu Ser Arg Leu Glu Glu Leu Ser Asp Glu Asp Ile 20 25 30 Asp Tyr Ser Asp Ile Pro Glu Leu Asp Asp Asn Phe Trp Glu Asn Ala 35 40 45 Glu Ile Val Asn Thr Asp Val Thr Gln Asn Thr Val Ser Ser Thr Leu 50 55 60 Gly Asn Phe 65

Claims

1. A use of a DNA fragment for preparing a nitrogen fixation enzyme, wherein: The nitrogen fixing enzyme allows the elimination of the need to add a required nitrogen source to a medium for the proliferation of Escherichia coli and allows the proliferation of Escherichia coli without adding Mo to the medium. The base sequence of the DNA fragment is SEQ ID NO:

1.

2. purposes according to claim 1, wherein said DNA fragment is derived from the genomic DNA of cyanobacteria.

3. The method according to claim 2, wherein the cyanobacteria is Cyanobacterium ATCC 51142.

4. Use of a recombinant vector for preparing a nitrogen fixation enzyme, wherein: The nitrogen fixing enzyme allows elimination of the need to add a required nitrogen source to a medium for proliferation of E. coli and allows proliferation of E. coli without adding Mo to the medium, and the recombinant vector comprises the DNA fragment according to any one of claims 1 to 3.

5. The use according to claim 4, wherein The recombinant vector includes a Fox plasmid vector containing the DNA fragment introduced therein.

6. Use of a transformant for preparing a nitrogen fixation enzyme, wherein: The nitrogen fixing enzyme allows elimination of the need to add a required nitrogen source to a medium for proliferation of E. coli and allows proliferation of E. coli without adding Mo to the medium, the transformant being transformed with the recombinant vector according to claim 4 or 5.

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