Sample solution tube and detection device

Abstract

The present invention provides a sample solution tube and a detection device, wherein the outer surface of the side wall of the sample solution tube is provided with at least one first protrusion, a truncated cone-shaped side wall and an external thread. The present invention also provides a detection device containing the sample solution tube, which is provided with a liquid storage tank, a liquid seal, a chromatography test paper and a puncture mechanism from bottom to top, and an opening is provided on the upper surface of the liquid storage tank, and the opening is sealed by a liquid seal, and the liquid seal can be opened. The adoption of this technical solution can better ensure the sealing effect, and because the sample solution tube is connected to the housing of the detection device by a threaded manner, the connection structure is more stable. After recording the test results, the liquid seal can be opened, and the chromatography test paper can react with the nucleic acid destruction reagent to completely remove the residual nucleic acid in the detection device.

Description

Technical Field

[0001] The present invention relates to the field of detection, and in particular to a sample solution tube and a detection device. Background Art

[0002] Nucleic acid diagnostics is one of the most dynamic segments of the IVD (in vitro diagnostic) industry in the future. The main drivers of nucleic acid diagnostics development in my country are the increased efforts in infectious disease prevention and control, the promotion of blood screening nucleic acid testing, and the development of personalized medicine. Driven by these factors, nucleic acid diagnostics in China is expected to grow at a rate of 25-30% in the future, significantly exceeding the average growth rate of the domestic IVD industry. Nucleic acid diagnostics will benefit large medical centers by enabling early, rapid, specific, and high-throughput detection of pathogens and genetic diseases.

[0003] Point-of-care testing (POCT), also known as "instant testing" in Chinese, is an emerging sub-sector of in vitro diagnostics (IVD). It enables analysis immediately at the point of sampling, eliminating the complex sample handling required for laboratory testing and providing rapid results. Key criteria for POCT are the lack of a fixed testing location, the portable reagents and instruments, and the ability to operate instantly. POCT assumes the functions of a laboratory without the traditional equipment of a hospital laboratory, providing comprehensive 24-hour patient service regardless of time or location.

[0004] However, these nucleic acid amplification methods are susceptible to cross-contamination of amplification products, which can cause false positive signals to misinterpret test results. Cross-contamination between samples is common during target nucleic acid amplification procedures. This contamination can originate from known or unknown positive substances introduced during the processing of negative samples, which can cause false positive reactions through air contamination or aerosols.

[0005] A series of methods have been developed in the prior art to prevent cross-contamination of amplification products. For example, Reference 1 (CN105199940A) discloses a contamination-proof portable gene detection method and device, through which a PCR tube containing amplification products can be placed in the device and sealed, and then the PCR tube can be punctured to achieve detection. This prevents contamination of nucleic acid amplification products and avoids false positives. However, in this device, since the PCR tube is placed in the device and sealed, the puncture operation is difficult, and the amplification product still remains in the device after the test is completed. Once damaged, the amplification product may also diffuse into the air, causing a false positive reaction.

[0006] In addition, reference 2 (CN203241416U) also discloses a closed plastic cartridge for chromatography test strips, and reference 3 (CN205574438U) also discloses a sealed test tube assembly containing a tube breaking mechanism. These detection devices are relatively convenient to operate, but the amplification product also remains in the device after detection, posing a risk of contamination.

[0007] Therefore, providing a nucleic acid detection device with a lower possibility of contamination before and after detection has become an urgent problem to be solved in this field. Summary of the Invention

[0008] The present invention aims to address the problems of conventional closed detection devices, which are inconvenient to operate and still present the possibility of contamination after detection. To address these issues, the present invention discloses a novel closed sample solution tube and closed detection device, which are easy to operate and effectively prevent amplification products from diffusing into the air, which could cause false positive reactions.

[0009] The present invention discloses a sample solution tube, comprising a side wall, a bottom wall and an upper wall, and a sealed sample solution storage chamber surrounded by the side wall, the bottom wall and the upper wall. The side wall comprises an upper side wall, a middle side wall and a lower side wall that are coaxial and connected in sequence. The upper side wall and the lower side wall are cylindrical, and the diameter of the upper side wall is larger than the diameter of the lower side wall. The middle side wall is a frustum, and the diameter gradually decreases from the end connected to the upper side wall to the end connected to the lower side wall. One or more first protrusions are provided on the outer surface of the middle side wall, and an external thread is provided on the outer surface of the lower side wall.

[0010] By adopting the above technical solution, the detection process is easier to operate and the possibility of leakage of the amplified product is smaller. The sealing effect can be better guaranteed by fitting the inclined surface with the first channel. In addition, since the sample solution tube is connected to the housing of the detection device by a threaded manner, the connection structure is more stable and not easy to fall off, further avoiding the exposure of the amplified product and the possibility of contamination.

[0011] According to another specific embodiment of the present invention, the upper portion of the sample solution tube is an open-cover structure or a closed structure.

[0012] According to another specific embodiment of the present invention, a sealing ring made of an elastomer is provided on the outside of the middle side wall of the sample solution tube.

[0013] The present invention also discloses a detection device, comprising a housing and any one of the aforementioned sample solution tubes,

[0014] The housing is provided with a receiving cavity, and the receiving cavity is provided with a liquid storage tank, a liquid seal, a chromatography test paper and a puncture mechanism in order from bottom to top. The upper surface of the liquid storage tank is provided with an opening, and the opening is sealed by a liquid seal. The liquid seal can be opened. The upper surface of the housing is provided with a first channel, and the first channel is configured to allow the sample solution tube to be inserted, thereby sealing the receiving cavity.

[0015] The first channel is provided with an inclined surface matching the side wall of the sample solution tube, and the inclined surface is provided with an annular first groove matching the first convex portion. When the sample solution tube is inserted into the first channel, the first convex portion and the first groove cooperate with each other to limit the axial movement of the sample solution tube relative to the first channel.

[0016] The puncture mechanism includes a cylindrical positioning portion and a puncture portion located on the central axis of the positioning portion. The puncture mechanism is also provided with a fluid channel. A third limiting portion is provided on the first channel below the inclined surface, and a fourth limiting portion is provided on the positioning portion. The third limiting portion and the fourth limiting portion cooperate with each other to limit the rotation of the puncture mechanism. An internal thread is provided on the inner surface of the positioning portion, and an external thread that cooperates with the internal thread is provided on the outer surface of the sample solution tube. When the sample solution tube is rotated, the puncture mechanism moves upward, puncturing the sample solution tube, and the sample solution flows from the fluid channel into the containing cavity.

[0017] By adopting the above technical solution, the accommodating chamber always remains sealed during the detection process, thus preventing the amplification product from leaking outward. Furthermore, after recording the test results, the liquid seal can be opened, and the chromatographic test paper can react with the nucleic acid destruction reagent to completely remove the nucleic acid remaining in the detection device. In this way, even if the sample solution tube accidentally falls off or the detection device is damaged in the subsequent process, causing internal exposure, it will not cause any pollution. The sample solution tube is allowed to remain stationary in the axial direction, and the puncture mechanism is moved upward by rotation to puncture the sample solution tube. During the puncture process, the air pressure in the shell remains unchanged, and the amplification product aerosol will not leak to the outside world, which can better avoid pollution. In addition, the sample solution tube and the shell are connected by threads, and the connection structure is more stable and not easy to fall off, further avoiding the possibility of amplification product exposure and contamination. Finally, a nucleic acid destruction reagent is used to remove the residual nucleic acid in the detection device, further avoiding possible contamination in the subsequent process.

[0018] According to another specific embodiment of the present invention, the third limiting portion is a second groove extending in the axial direction, and the fourth limiting portion is a second protrusion.

[0019] According to another specific embodiment of the present invention, the liquid seal can be moved to open the liquid storage tank, a third protrusion is provided at the lower end of the positioning portion, and a corresponding third groove is provided on the liquid seal. Before the puncturing mechanism moves upward, the third protrusion is arranged in the third groove.

[0020] According to another specific embodiment of the present invention, an elastic downward pressing structure is provided above the chromatography test paper. When the liquid seal is opened, the elastic downward pressing structure presses at least part of the chromatography test paper into the liquid storage tank.

[0021] According to another specific embodiment of the present invention, a sealing ring made of an elastomer is provided at the insertion end of the first channel, or a sealing ring made of an elastomer is provided on the side wall of the sample solution tube.

[0022] According to another specific embodiment of the present invention, a transparent area is further provided on the housing for observing the detection results.

[0023] According to another embodiment of the present invention, a nucleic acid destructive reagent is stored in the liquid storage tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments:

[0025] Figure 1 is a cross-sectional view of a sample solution tube provided by the present invention;

[0026] Figure 2 This is a schematic structural diagram of a sample solution tube provided by the present invention;

[0027] Figure 3 This is a cross-sectional view of the sample solution tube and the detection device provided by the present invention in a matching state;

[0028] Figure 4 is a cross-sectional view of another matching state of the sample solution tube and the detection device provided by the present invention;

[0029] Figure 5 This is a schematic structural diagram of the sample solution tube and the detection device provided by the present invention in a coordinated state;

[0030] Figure 6 This is a schematic diagram of an explosion of a sample solution tube and a detection device provided by the present invention;

[0031] Figure 7 It is a structural schematic diagram of the puncture mechanism provided by the present invention;

[0032] Figure 8 It is a structural schematic diagram of the puncture mechanism provided by the present invention.

[0033] Reference numerals:

[0034] Sample solution tube 100

[0035] Side wall 110

[0036] Upper side wall 111

[0037] Middle side wall 112

[0038] Lower side wall 113

[0039] first convex portion 114

[0040] Bottom wall 120

[0041] Recess 121

[0042] Upper wall 130

[0043] Sample solution storage chamber 140

[0044] Sealing ring 150

[0045] Housing 200

[0046] Accommodating chamber 210

[0047] Liquid storage tank 220

[0048] Liquid seal 230

[0049] Chromatography test paper 240

[0050] Puncture mechanism 250

[0051] Positioning portion 251

[0052] Fourth limiting portion 2511

[0053] The third convex part 2512

[0054] Piercing portion 252

[0055] Fluid channel 253

[0056] First channel 260

[0057] First groove 261

[0058] The third limiting portion 262

[0059] Transparent area 270

[0060] Elastic downward pressure structure 280 DETAILED DESCRIPTION

[0061] The following is an explanation of the embodiments of the present invention by specific specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the present invention will be introduced in conjunction with the preferred embodiment, this does not mean that the features of this invention are limited to this embodiment. On the contrary, the purpose of introducing the invention in conjunction with the embodiment is to cover other options or modifications that may be extended based on the claims of the present invention. In order to provide a deep understanding of the present invention, the following description will contain many specific details. The present invention can also be implemented without using these details. In addition, in order to avoid confusion or blurring the focus of the present invention, some specific details will be omitted in the description. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0062] In the description of this embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the inventive product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0063] The terms "first", "second", "third", etc. are only used for distinction and description and should not be understood as indicating or implying relative importance.

[0064] In the description of this embodiment, it should be noted that, unless otherwise specified or limited, the terms "disposed," "connected," and "connected" should be understood broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this embodiment based on specific circumstances.

[0065] To make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0066] like Figure 1 and Figure 2As shown, the present invention discloses a sample solution tube 100, including a side wall 110, a bottom wall 120 and an upper wall 130, and a sealed sample solution storage chamber 140 surrounded by the side wall 110, the bottom wall 120 and the upper wall 130. The side wall 110 includes an upper side wall 111, a middle side wall 112 and a lower side wall 113 that are coaxial and connected in sequence. The upper side wall 111 and the lower side wall 113 are cylindrical, and the diameter of the upper side wall 111 is larger than the diameter of the lower side wall 113. The middle side wall 112 is a frustum, and the diameter gradually decreases from the end connected to the upper side wall 111 to the end connected to the lower side wall 113.

[0067] One or more first protrusions 114 are provided on the outer surface of the middle side wall 112. If there are multiple first protrusions 114, the multiple first protrusions 114 can be located on the same cross-section of the middle side wall 112. An external thread (not shown in the figure) is provided on the outer surface of the lower side wall 113, and the external thread is located below the first protrusion 114.

[0068] The structure of the first protrusion 114 is not particularly limited, as long as it can cooperate with the first groove 261 to limit the position of the sample solution tube 100 relative to the first channel 260 in the axial direction. The first protrusion 114 can be a raised point, a circumferentially extending raised strip, or a complete circular raised ring. From the perspective of more effectively and stably limiting the axial position of the sample solution tube 100, the first protrusion 114 is preferably a complete circular raised ring, or a plurality of raised points or raised strips evenly distributed along the circumference of the sidewall 110.

[0069] According to another specific embodiment of the present invention, Figure 1 and Figure 2 As shown, the first protrusion 114 on the side wall 112 of the sample solution tube 100 is a circular protrusion ring, and the lower side wall 110 of the sample solution tube 100 below the first protrusion 114 is a cylindrical side wall, which is provided with an external thread that cooperates with the internal thread of the positioning portion 251 of the puncture mechanism 250.

[0070] According to another specific embodiment of the present invention, the sample solution tube 100 of the present invention is a specially made PCR tube. The side wall 110 of the sample solution tube 100 can be made of a series of materials, preferably made of materials with good thermal conductivity, high strength, and good fluidity, such as metals, alloys, thermally conductive plastics, and organic composite materials. The height is 1-3 cm, preferably 2 cm. The general shape can be similar to that of an ordinary PCR tube, but there are some differences.

[0071] According to another specific embodiment of the present invention, in order to facilitate the puncturing of the sample solution tube 100 by the puncturing mechanism 250 after being inserted into the detection device, a cut may be provided on the bottom wall 120 of the sample solution tube 100. When the puncturing mechanism 250 punctures the bottom wall 120 of the sample solution tube 100, the bottom wall 120 of the sample solution tube 100 breaks along the position of the cut; or the bottom wall 120 may be set to be thinner relative to other parts of the sample solution tube 100 to facilitate the puncturing mechanism 250 to puncture the sample solution tube 100.

[0072] Furthermore, because the bottom wall 120 of the sample solution tube 100 is provided with a notch or is configured to be relatively thin, the sample solution tube 100 may be damaged along the notch or the thinner portion during the removal and placement process before being inserted into the detection device. Therefore, according to another specific embodiment of the present invention, the bottom wall 120 of the sample solution tube 100 is provided with a recess 121, and the notch is provided within the recess 121; or the wall of the sample solution tube 100 is thinner only within the recess 121, preferably at least at the center of the bottom wall 120 of the sample solution tube 100. This effectively prevents damage to the sample solution tube 100 during removal and placement.

[0073] According to another specific embodiment of the present invention, the general sample solution tube 100 is an open-cover structure, that is, the upper wall 130 is an openable lid. After the sample to be amplified and the reagents related to the amplification reaction system are placed in the sample solution tube 100, the lid is closed to achieve sealing. In addition, the upper part of the sample solution tube 100 can also be directly a closed structure, that is, the upper wall 130 and the side wall 110 are fixedly connected, or even directly integrally formed and cannot be opened. When in use, a syringe with a fine needle pierces the upper wall 130 of the sample solution tube 100, injects the reaction system, and then seals the rupture with a sealing film or a wax drop with a higher melting point, so that the sealing of the sample solution tube 100 can be better achieved.

[0074] Furthermore, certain impoverished or underdeveloped areas have been hardest hit by infectious diseases due to poverty, poor sanitary conditions, low hygiene awareness, malnutrition, and other factors. Infectious diseases have high morbidity and mortality rates, and the high cost of treatment is unaffordable for ordinary families. However, advanced infectious disease testing methods are not widely available in these areas. The main reasons are that most areas have difficulty in obtaining electricity, making it impossible to operate large instruments; they cannot afford the cost of large medical equipment and the corresponding maintenance costs; they are limited by space; and patients cannot afford the high cost of testing. The amplification reaction in the sample solution tube 100 needs to be carried out within a specific temperature range. In these areas, it may be difficult for testers to obtain temperature control equipment, and on-site testing cannot be performed, which limits the immediacy of nucleic acid testing.

[0075] According to another specific embodiment of the present invention, the surface of the sample solution tube 100 is coated with at least two reversible temperature-sensitive color-changing materials. The color change temperature of the temperature-sensitive color-changing material can be set according to actual needs, and the specific reversible temperature-sensitive color-changing material can be a commercially available product. For a certain amplification reaction, if the reaction temperature is required to be between the first temperature T1 and the second temperature T2, two temperature-sensitive color-changing materials can be coated on the surface of the sample solution tube 100, the color change temperature of the first temperature-sensitive color-changing material being the first temperature T1, and the color change temperature of the second temperature-sensitive color-changing material being the second temperature T2. In this way, when performing this amplification reaction, when the first temperature-sensitive material changes color and the second temperature-sensitive material does not change color, it indicates that the temperature is just suitable for the amplification reaction in the sample solution tube 100. In this way, the sample solution tube 100 can be directly placed in a thermos, and the amount of hot and cold water can be adjusted to control the temperature of the water in the thermos to maintain the progress of the amplification reaction. No constant temperature equipment is required, and the sample can be tested anytime and anywhere.

[0076] For example, if the optimal reaction temperature is around 38 degrees, two temperature-sensitive coatings can be used for temperatures greater than 38 and less than 38 degrees, preferably 37 and 39 degrees. If the optimal reaction temperature is 63 degrees, the temperature-sensitive coatings can be selected as 62 and 64. The shape of the temperature-sensitive coating can be any, but preferably, the Arabic numeral corresponding to the temperature, such as a temperature-sensitive material that changes color at 38 degrees will display "38". This allows for a more direct reaction to the temperature of the sample solution tube 100.

[0077] Furthermore, if the sample solution tube 100 is used in amplification reaction systems with different temperatures, a variety of temperature-sensitive color-changing materials can be provided.

[0078] According to another specific embodiment of the present invention, Figures 3 to 6 As shown, the present invention also discloses a detection device, comprising a housing 200 and any one of the aforementioned sample solution tubes 100,

[0079] The housing 200 is provided with a accommodating chamber 210, and the accommodating chamber 210 is provided with a liquid storage tank 220, a liquid seal 230, a chromatography test paper 240 and a puncture mechanism 250 from bottom to top. The upper surface of the liquid storage tank 220 is provided with an opening, and the opening is sealed by the liquid seal 230. The liquid seal 230 can be opened. The liquid storage tank 220 can store a nucleic acid destructing reagent, such as sodium hypochlorite solution or a commercial DNA detergent.

[0080] A cylindrical first channel 260 is provided on the upper surface of the housing 200. The first channel 260 is configured for inserting the sample solution tube 100. The shape of the first channel 260 matches the sample solution tube 100 to seal the accommodating chamber 210. The first channel 260 is provided with an inclined surface that matches the side wall 112 of the sample solution tube 100. The inclined surface is provided with an annular first groove 261 that matches the first protrusion 114. When the sample solution tube 100 is inserted into the first channel 260, the first protrusion 114 and the first groove 261 cooperate with each other to limit the axial movement of the sample solution tube 100 relative to the first channel 260.

[0081] like Figure 7 and Figure 8 As shown, the puncturing mechanism 250 includes a cylindrical positioning portion 251 and a puncturing portion 252 located on the central axis of the positioning portion 251. The puncturing mechanism 250 is also provided with a fluid channel 253, and the first channel 260 is provided with a third limiting portion 262. The positioning portion 251 is provided with a fourth limiting portion 2511. The third limiting portion 262 and the fourth limiting portion 2511 cooperate with each other to limit the puncturing mechanism 250 from rotating about the central axis of the positioning portion 251. An internal thread (not shown in the figure) is provided on the inner surface of the positioning portion 251, and an external thread that cooperates with the internal thread is provided on the outer surface of the sample solution tube 100.

[0082] When the sample solution tube 100 is inserted into the first channel 260 and the sample solution tube 100 is rotated, as shown in FIG. Figure 3 As shown, due to the cooperation between the first protrusion 114 and the annular first groove 261, the sample solution tube 100 can only rotate, but cannot be inserted inward relative to the first channel 260. And as the sample solution tube 100 rotates, due to the cooperation between the external thread on the side wall 110 of the sample solution tube 100 and the internal thread on the positioning portion 251, the positioning portion 251 will drive the piercing portion 252 to move toward the sample solution tube 100. Figure 4 The location shown ( Figure 4 The liquid seal 230 has been opened, but the liquid seal 230 cannot be opened before the detection is completed. Figure 4 (To illustrate the relative positional relationship between the puncturing mechanism 250 and the sample solution tube 100), the sample solution tube 100 is punctured, and the sample solution flows from the fluid channel 253 into the receiving chamber 210. The chromatography test paper 240 in the receiving chamber 210 can then test the sample, and the tester can observe the test results in the test result viewing area 270.

[0083] By adopting the above technical solution, during the detection process, the accommodating chamber 210 is always kept sealed, thus preventing the amplification product from leaking outward. Figure 4As shown, the liquid seal 230 can be opened, and the chromatography test paper 240 can react with the nucleic acid destruction reagent to completely remove the residual nucleic acid in the detection device. In this way, even if the sample solution tube 100 is accidentally removed during the subsequent process, or the detection device is damaged, causing the internal parts to be exposed, no contamination will occur.

[0084] In addition, in the prior art, when the sample solution tube 100 is inserted downward and the puncture mechanism 250 remains stationary, the gas in the shell 200 is compressed, and the amplified product may leak from the gap between the sample solution tube 100 and the shell 200, causing contamination. However, the closed detection device with a new structure provided by the present invention allows the sample solution tube 100 to remain stationary in the axial direction, but punctures the sample solution tube 100 by rotating the puncture mechanism 250 upward. During the puncture process, the air pressure in the shell 200 remains unchanged, and the amplified product aerosol will not leak to the outside world, which can better avoid contamination. In addition, the sample solution tube 100 and the shell 200 are connected by threads, and the connection structure is more stable and not easy to fall off, further avoiding the possibility of exposure of the amplified product and causing contamination. Finally, a nucleic acid destruction reagent is used to remove the residual nucleic acid in the detection device, further avoiding possible contamination in the subsequent process.

[0085] According to another specific embodiment of the present invention, the third stopper 262 is a second groove extending axially, and the fourth stopper 2511 is a second protrusion. The second protrusion can be a raised dot or an axially extending ridge; the length of the second groove allows the piercing mechanism 250 to move upward to the position where it pierces the sample solution tube 100. Before the sample solution tube 100 is inserted into the positioning portion 251 and begins to rotate, the second protrusion is located at the lowest end of the second groove. As the sample solution tube 100 rotates, the entire piercing mechanism 250 moves upward, and the second protrusion moves upward along the second groove until the piercing mechanism 250 pierces the bottom wall 120 of the sample solution tube 100.

[0086] Furthermore, from a more reliable perspective, multiple second protrusions and second grooves extending axially in coordination therewith may be provided. Preferably, the multiple second protrusions and second grooves extending axially in coordination therewith are evenly distributed along the circumference of the first channel 260 .

[0087] It is easy to imagine that the third limiting portion 262 can also be set as a second protrusion, and the fourth limiting portion 2511 can be set as a second groove extending along the axial direction.

[0088] According to another specific embodiment of the present invention, Figures 3 to 6As shown, the housing 200 is provided with a through hole, and the liquid seal 230 is provided on the housing 200 through the through hole. The liquid seal 230 includes an operating portion located outside the housing 200 and a sealing portion located inside the housing 200. The sealing portion seals the upper surface of the liquid storage tank 220, and the liquid seal 230 has a first position and a second position. When the detection device is not in use, the liquid seal 230 is in Figure 3 The first position shown in FIG. 1 is a first position in which the liquid seal 230 can be moved to the position indicated by the operating portion. Figure 4 In the second position shown, the liquid storage tank 220 is opened. During the entire process, the liquid seal 230 is always sealed and connected to the housing 200, the accommodating chamber 210 is always in a sealed state, and the amplification product will not leak.

[0089] According to another specific embodiment of the present invention, a third protrusion 2512 is provided at the lower end of the positioning portion 251, and a corresponding third groove is provided on the liquid seal 230. Before the puncturing mechanism 250 moves upward, the third protrusion 2512 is arranged in the third groove. Due to the cooperation between the third protrusion 2512 and the third groove, the liquid seal 230 is always in the first position and cannot be moved to the second position. After the sample solution tube 100 is inserted into the positioning portion 251 and the puncturing mechanism 250 is rotated and lifted, the third protrusion 2512 also moves upward and disengages from the third groove. At this time, the inspector can move the liquid seal 230 from the first position to the second position through the operating portion, open the liquid storage tank 220, and the chromatography test paper 240 can come into contact with the destructive liquid in the liquid storage tank 220.

[0090] According to another embodiment of the present invention, an elastic pressing structure 280 is provided above the chromatography test paper 240. When the liquid seal 230 is opened, the elastic pressing structure 280 presses at least a portion of the chromatography test paper 240 into the liquid storage tank 220. The elastic pressing structure 280 is not particularly limited and can be a spring or a spring.

[0091] Furthermore, the specific structure for achieving sealing of the receiving chamber 210 can refer to any existing method in the prior art and will not be elaborated in detail in the present invention. For example, the first channel 260 can be shaped to match the sample solution tube 100. After the sample solution tube 100 is inserted into the first channel 260, the surfaces of the two come into contact with each other, thereby achieving sealing of the receiving chamber 210. According to another specific embodiment of the present invention, in order to more effectively prevent the expansion product from leaking into the air, a sealing ring made of an elastomer can be provided at the insertion end of the first channel 260, or a sealing ring made of an elastomer can be provided on the side wall 110 of the sample solution tube 100, such as a sealing ring 150 provided on the middle side wall 111 of the sample solution tube 100.

[0092] According to another specific embodiment of the present invention, a transparent area 270 is further provided on the housing for observing the detection results.

[0093] In summary, through the closed detection device with a new structure provided by the present invention, the accommodating chamber always remains sealed during the detection process, thereby preventing the amplification product from leaking outward. Furthermore, the device allows the sample solution tube to remain stationary in the axial direction, while the puncture mechanism is rotated to move upward to puncture the sample solution tube. During the puncture process, the air pressure in the shell remains unchanged, and the amplification product aerosol will not leak to the outside world, which can better avoid contamination. In addition, the sample solution tube and the shell are connected by threads, and the connection structure is more stable and not easy to fall off, further avoiding the possibility of amplification product exposure and contamination. Finally, a nucleic acid destruction reagent is used to remove the residual nucleic acid in the detection device, further avoiding possible contamination in subsequent processes.

[0094] Although the present invention has been illustrated and described with reference to certain preferred embodiments thereof, it should be understood by those skilled in the art that the above description is provided as a further detailed description of the present invention in conjunction with specific embodiments thereof, and that the specific implementation of the present invention is not limited to these descriptions. Those skilled in the art may make various changes in form and details, including simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims

1. A detection device, characterized in that: Including housing and sample solution tube, The sample solution tube includes a side wall, a bottom wall and an upper wall, and a sealed sample solution storage chamber surrounded by the side wall, the bottom wall and the upper wall. The side wall includes an upper side wall, a middle side wall and a lower side wall that are coaxial and connected in sequence. The upper side wall and the lower side wall are cylindrical, and the diameter of the upper side wall is larger than the diameter of the lower side wall. The middle side wall is frustum-shaped, and the diameter gradually decreases from the end connected to the upper side wall to the end connected to the lower side wall. One or more first protrusions are provided on the outer surface of the middle side wall, and an external thread is provided on the outer surface of the lower side wall. The upper part of the sample solution tube is an open cover structure or a closed structure. The housing is provided with a receiving cavity, and the receiving cavity is provided with a liquid storage tank, a liquid sealing member, a chromatography test paper and a puncture mechanism in order from bottom to top. The upper surface of the liquid storage tank is provided with an opening, and the opening is sealed by the liquid sealing member, and the liquid sealing member can be opened. The upper surface of the housing is provided with a first channel, which is configured to allow the sample solution tube to be inserted and seal the accommodating cavity. The first channel is provided with an inclined surface that matches the side wall of the sample solution tube, and the inclined surface is provided with an annular first groove that matches the first protrusion. When the sample solution tube is inserted into the first channel, the first protrusion and the first groove cooperate with each other to limit the axial movement of the sample solution tube relative to the first channel. The puncture mechanism includes a cylindrical positioning portion and a puncture portion located on the central axis of the positioning portion. The puncture mechanism is also provided with a fluid channel. A third limiting portion is further provided on the first channel at a position below the inclined surface. A fourth limiting portion is provided on the positioning portion. The third limiting portion and the fourth limiting portion cooperate with each other to limit the rotation of the puncture mechanism. An internal thread is provided on the inner surface of the positioning portion, and an external thread that cooperates with the internal thread is provided on the outer surface of the sample solution tube. When the sample solution tube is rotated, the puncture mechanism moves upward to puncture the sample solution tube, and the sample solution flows from the fluid channel into the accommodating cavity. A sealing ring made of elastic body is arranged outside the middle side wall of the sample solution tube.

2. The detection device according to claim 1, wherein The third limiting portion is a second groove extending in the axial direction, and the fourth limiting portion is a second convex portion.

3. The detection device according to claim 1, wherein The liquid seal can be moved to open the liquid storage tank. A third protrusion is provided at the lower end of the positioning portion, and a corresponding third groove is provided on the liquid seal. Before the puncture mechanism moves upward, the third protrusion is arranged in the third groove.

4. The detection device according to claim 1, wherein An elastic downward pressing structure is provided above the chromatography test paper. When the liquid sealing member is opened, the elastic downward pressing structure presses at least part of the chromatography test paper into the liquid storage tank.

5. The detection device according to claim 1, wherein A sealing ring made of elastomer is also provided in the first channel.

6. The detection device according to claim 1, wherein The shell is also provided with a transparent area for observing the test results.

7. The detection device according to claim 1, wherein: The liquid storage tank contains a nucleic acid destruction reagent.

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