Liquid-based cell automatic slide making machine

Abstract

The liquid-based cell automatic slide making machine relates to the field of experimental instruments, and solves the problems of the existing slide making machines being expensive in price and consumables, and the low efficiency and poor quality of manual slide making. The present invention comprises: a pipette tip box, a specimen collection bottle box, and a glass slide box arranged on a machine base; two Y-axis motion mechanisms installed on the left and right sides of the upper surface of the machine base; a support frame, the two ends of which are respectively fixed on the two Y-axis motion mechanisms; an X-axis motion mechanism installed on the support frame; a Z-axis motion mechanism installed on the X-axis motion mechanism; a test tube rack rotating drive mechanism installed inside the machine base; a test tube rack fixed on the upper end of the test tube rack rotating drive mechanism and located in the central through hole of the machine base, the outer ring and inner ring of the test tube rack are respectively provided with a plurality of evenly distributed card slots; a centrifuge tube installed in the card slot of the outer ring of the test tube rack; and a test tube installed in the card slot of the inner ring of the test tube rack. The present invention is easy to operate, safe and reliable, energy-saving and environmentally friendly, low in cost, and high in slide making quality and efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of test instruments, and in particular to a liquid-based cell full-automatic slicing machine. Background Art

[0002] The development of advanced slide making technology has not only increased the number of readable cells, but also removed impurities such as red blood cells, mucus and cell debris that were previously present in PAP smears and hindered the observation and diagnosis of the films. The cells are evenly distributed in a thin layer, the background is clear, and the cell nuclei are clearly visible, making the tested cell smears easier for readers to read and diagnose, effectively reducing their visual fatigue, improving the reading effect and increasing the diagnosis rate of cytological lesions.

[0003] At present, the existing fully automatic slide making machines are expensive and consumables are expensive, so many hospitals still use traditional manual slide making. However, this manual slide making method has many disadvantages and shortcomings. Due to the different techniques of operators and the non-standard operation process, it is easy to lead to a high false positive and false negative rate. In addition, manual slide making is inefficient and of poor quality, which cannot meet the growing diagnostic needs. Summary of the invention

[0004] In order to solve the problems of high price and consumables of existing fully automatic slide making machines, low efficiency and poor quality of manual slide making, the present invention provides a liquid-based cell fully automatic slide making machine.

[0005] The technical solution adopted by the present invention to solve the technical problem is as follows:

[0006] The liquid-based cell automatic slide making machine of the present invention comprises: a machine base with a hollow interior, an outer cover fixed on the machine base, and a door installed in a front end slide groove of the outer cover, wherein a through hole is provided at the center of the upper surface of the machine base, and further comprises:

[0007] A pipette tip box, a specimen collection bottle box, and a glass slide box are all arranged on the upper surface of the machine base, wherein the pipette tip box is used to place the pipette tip, the specimen collection bottle box is used to place the specimen collection bottle, and the glass slide box is used to place the glass slide;

[0008] A first Y-axis motion mechanism and a second Y-axis motion mechanism are respectively mounted on the left and right sides of the upper surface of the machine base;

[0009] A supporting frame, both ends of which are respectively fixed to the first Y-axis motion mechanism and the second Y-axis motion mechanism;

[0010] An X-axis motion mechanism mounted on a support frame;

[0011] A Z-axis motion mechanism mounted on an X-axis motion mechanism;

[0012] A test tube rack rotation drive mechanism installed inside the machine base;

[0013] A test tube rack fixed to the upper end of the test tube rack rotation drive mechanism, the test tube rack is located in the central through hole of the machine base, and the outer ring and inner ring of the test tube rack are respectively provided with a plurality of evenly distributed slots;

[0014] A centrifuge tube installed in the outer ring slot of the test tube rack;

[0015] The test tube is installed in the inner ring groove of the test tube rack.

[0016] Furthermore, it also includes:

[0017] Switches, USB ports and video monitors are all located on the front surface of the base;

[0018] The waste liquid tank, camera and control system are all set inside the base;

[0019] The X-axis motion mechanism, the Z-axis motion mechanism, the first Y-axis motion mechanism, the second Y-axis motion mechanism, the switch, the USB interface, the video monitor, and the camera are all electrically connected to the control system.

[0020] Furthermore, it also includes:

[0021] The two ends are respectively fixed to the transverse guide rail on the support frame, and the lower end of the X-axis motion mechanism is installed on the transverse guide rail.

[0022] Furthermore, the X-axis motion mechanism comprises:

[0023] An X-axis movable card seat, wherein mounting holes are respectively arranged on both sides of the upper end of the X-axis movable card seat, and the support frame extends into the two mounting holes on both sides of the upper end of the X-axis movable card seat; mounting holes are respectively arranged on both sides of the lower end of the X-axis movable card seat, and the transverse guide rail extends into the mounting holes on both sides of the lower end of the X-axis movable card seat;

[0024] Z-axis synchronous toothed belts are respectively arranged on the inner walls of both sides of the front opening of the X-axis moving card seat;

[0025] Dovetail grooves are respectively arranged on the outer walls of both sides of the front opening of the X-axis moving card seat;

[0026] An X-axis moving servo motor fixed inside the X-axis moving card holder and electrically connected to the control system;

[0027] An X-axis moving driving gear fixedly connected to the output shaft of the X-axis moving servo motor via a flat key;

[0028] An X-axis synchronous toothed belt disposed on the support frame, wherein the X-axis synchronous toothed belt is meshed with the X-axis moving drive gear;

[0029] The X-axis moving servo motor is started to drive the X-axis moving driving gear to rotate, and the entire X-axis moving card seat is driven to move along the X-axis on the supporting frame through the meshing action between the X-axis moving driving gear and the X-axis synchronous toothed belt.

[0030] Furthermore, the Z-axis motion mechanism comprises:

[0031] Z-axis moving card holder;

[0032] A Z-axis moving servo motor fixed to the upper end of the front surface of the Z-axis moving card holder and electrically connected to the control system;

[0033] A manipulator absorber is installed in the mounting hole at the lower end of the front surface of the Z-axis moving card seat, and the upper end of the manipulator absorber is fixedly connected to the output shaft of the Z-axis moving servo motor;

[0034] A straw socket is provided at the lower end of the manipulator adsorber, wherein a straw is installed in the straw socket;

[0035] Dovetail structures are respectively arranged on both sides of the rear surface of the Z-axis moving card seat, and the dovetail structures are installed in a one-to-one correspondence with the dovetail grooves;

[0036] A Z-axis moving drive gear is arranged at the upper end of the rear surface of the Z-axis moving card seat, and the Z-axis moving drive gear is installed in the lower end opening of the X-axis moving card seat, and the Z-axis moving drive gear is meshed with the Z-axis synchronous toothed belt;

[0037] The Z-axis moving servo motor is started to drive the Z-axis moving driving gear to rotate, and the entire Z-axis moving card seat and the manipulator absorber are driven to move along the Z axis on the X-axis moving card seat through the meshing action between the Z-axis moving driving gear and the Z-axis synchronous toothed belt.

[0038] Further, the first Y-axis motion mechanism includes: a first Y-axis moving card holder, a first Y-axis moving servo motor, a first Y-axis moving driving gear, a first rack and a first linear guide rail;

[0039] The first rack and the first linear guide are both fixed to the left side of the upper surface of the machine base, and the first rack is located outside the first linear guide; the lower end of the left side of the support frame is fixed to the first Y-axis movable card seat; one side of the lower end of the first Y-axis movable card seat is installed on the first linear guide, and the other side of the lower end of the first Y-axis movable card seat is set in an open form, the first Y-axis movable servo motor is fixed inside the opening of the first Y-axis movable card seat, the first Y-axis movable servo motor is electrically connected to the control system, the first Y-axis movable drive gear is fixedly connected to the output shaft of the first Y-axis movable servo motor through a flat key, and the first Y-axis movable drive gear and the first rack are meshed with each other;

[0040] The first Y-axis moving servo motor is started to drive the first Y-axis moving driving gear to rotate, and the entire first Y-axis moving card seat is driven to move along the Y-axis direction on the first linear guide rail through the meshing action between the first Y-axis moving driving gear and the first rack.

[0041] Furthermore, the second Y-axis motion mechanism includes: a second Y-axis moving card seat, a second Y-axis moving servo motor, a second Y-axis moving driving gear, a second rack and a second linear guide; the second rack and the second linear guide are both fixed to the right side of the upper surface of the machine base, and the second rack is located outside the second linear guide; the lower end of the right side of the support frame is fixed to the second Y-axis moving card seat; one side of the lower end of the first Y-axis moving card seat is installed on the second linear guide, and the other side of the lower end of the second Y-axis moving card seat is set in an open form, the second Y-axis moving servo motor is fixed inside the opening of the second Y-axis moving card seat, the second Y-axis moving servo motor is electrically connected to the control system, the second Y-axis moving driving gear is fixedly connected to the output shaft of the second Y-axis moving servo motor, and the second Y-axis moving driving gear and the second rack are meshed with each other;

[0042] The second Y-axis moving servo motor is started to drive the second Y-axis moving driving gear to rotate, and the entire second Y-axis moving card seat is driven to move along the Y-axis direction on the second linear guide rail through the meshing action between the second Y-axis moving driving gear and the second rack.

[0043] Further, the test tube rack rotation drive mechanism includes: a vertical shaft, a first ball bearing, a driving gear, a passive gear, a rotation servo motor, a second ball bearing, a thrust bearing, a rotation servo motor fixing seat and a bearing seat;

[0044] The rotating servo motor fixing seat and the bearing seat are both fixed inside the machine base; the test tube rack is fixed on the upper end of the vertical shaft; the thrust bearing, the second ball bearing, the driving gear, and the first ball bearing are sequentially installed on the lower end of the vertical shaft from bottom to top; the outer diameter of the lower end of the vertical shaft is transitionally matched with the inner hole of the thrust bearing, and the lower end face of the thrust bearing contacts with the bearing seat inside the machine base. The thrust bearing mainly bears the axial force, and the upper end face of the thrust bearing contacts with the inner ring of the second ball bearing. The inner holes of the second ball bearing and the first ball bearing are both transitionally matched with the outer diameter of the vertical shaft. The second ball bearing and the first ball bearing both rotate together with the vertical shaft. The outer diameter of the second ball bearing and the first The outer diameters of the ball bearings are fixedly connected to the bearing seats inside the machine base; the driving gear is installed between the second ball bearing and the first ball bearing, and the inner diameter of the driving gear is dynamically matched with the outer diameter of the vertical shaft; the rotary servo motor is fixed on the rotary servo motor fixing seat inside the machine base, and the rotary servo motor is electrically connected to the control system; the driving gear is meshed with the passive gear, and the inner hole of the rotary driving gear is tightly matched with the rotary servo motor shaft; when the rotary servo motor rotates, the passive gear drives the driving gear to rotate together, and the passive gear meshes and rotates with the driving gear, driving the vertical shaft to rotate, and at the same time drives the test tube rack on the vertical shaft to rotate together.

[0045] Furthermore, a resistance wire is provided in the slide box.

[0046] Furthermore, it also includes a ring arranged on the outer circle of the central through hole of the base.

[0047] The beneficial effects of the present invention are as follows: the liquid-based cell fully automatic slide making machine of the present invention is a fully automatic machine for processing and making conventional in vitro cytological specimens, and is widely applicable to the making of various cytological specimens of the human body.

[0048] The present invention adopts a centrifugal and slide-making integrated design, which greatly improves the efficiency and quality of slide making. Only by putting the cell specimen collection bottle into the machine, the slide making machine will automatically complete a series of fully automatic slide making processes such as cell mixing, sampling and centrifugation, making the processing of cytological samples more accurate, reducing the errors caused by manual operation, and improving work efficiency. The good effect has been verified by experiments.

[0049] The present invention integrates mechanical, electrical and pneumatic functions, and has multiple innovative features in structures and parts such as liquid-based centrifugation, cell analysis, slide heating, liquid-based transfer, manipulator positioning control, and cell adhesion. It overcomes the shortcomings of unstable quality and poor effect of manual slide making and realizes automated slide making.

[0050] The invention is easy to operate, safe and reliable, energy-saving and environmentally friendly, low-cost, and significantly improves the quality of film making. The film making efficiency is more than 10 times higher than that of manual film making, and is suitable for domestic small and medium-sized hospitals. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 It is a schematic diagram of the structure of the liquid-based cell automatic slide making machine of the present invention.

[0052] Figure 2 The figure is a schematic diagram of the internal structure of the liquid-based cell automatic slide making machine of the present invention.

[0053] Figure 3 It is a structural schematic diagram of the test tube rack rotation drive mechanism.

[0054] Figure 4 It is a structural diagram of the X-axis motion mechanism.

[0055] Figure 5 It is a structural diagram of the X-axis motion mechanism.

[0056] Figure 6 It is a structural diagram of the Z-axis motion mechanism.

[0057] Figure 7 It is a structural diagram of the Z-axis motion mechanism.

[0058] Figure 8 It is a structural diagram of the Y-axis motion mechanism.

[0059] Fig. 9 Schematic diagram of the installation of test tube racks, centrifuge tubes and test tubes.

[0060] Fig.10 It is a schematic diagram of the structure of the liquid-based cell automatic slide making machine of the present invention.

[0061] In the figure, 1, X-axis motion mechanism, 2, Z-axis motion mechanism, 3, first Y-axis motion mechanism, 4, second Y-axis motion mechanism, 5, support frame, 6, transverse guide rail, 7, machine base, 8, first rack, 9, second rack, 10, first linear guide rail, 11, second linear guide rail, 12, ring, 13, test tube rack, 14, centrifuge tube, 15, pipette tip box, 16, specimen collection bottle box, 17, slide box, 18, switch, 19, USB interface, 20, video monitor, 21, outer cover, 22, door, 23, vertical shaft, 24, first ball bearing, 25, driving gear, 26, passive gear, 27, rotary servo motor, 28, second ball bearing, 29, thrust bearing, 30, test tube. DETAILED DESCRIPTION

[0062] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0063] like Figure 1 , Figure 2 and Figure 3 As shown, the liquid-based cell automatic slide making machine of the present invention mainly includes: an X-axis motion mechanism 1, a Z-axis motion mechanism 2, a first Y-axis motion mechanism 3, a second Y-axis motion mechanism 4, a support frame 5, a transverse guide rail 6, a base 7, a ring 12, a test tube rack 13, a centrifuge tube 14, a pipette tip box 15, a specimen collection bottle box 16, a slide box 17, a switch 18, a USB interface 19, a video monitor 20, an outer cover 21, a door 22, a test tube rack rotation drive mechanism, a test tube 30, a waste liquid tank, a camera, and a control system.

[0064] The base 7 is hollow inside. A through hole is provided at the center of the upper surface of the base 7. A ring 12 is provided on the outer ring of the central through hole. A test tube rack 13 is located in the central through hole of the base 7. The lower end of the test tube rack 13 is mounted on the test tube rack rotation drive mechanism. The outer ring and the inner ring of the test tube rack 13 are respectively provided with a plurality of evenly distributed slots. The centrifuge tube 14 is installed in the outer ring slot of the test tube rack 13, and the test tube 30 is installed in the inner ring slot of the test tube rack 13. Fig. 9 shown.

[0065] The outer cover 21 is fixed on the machine base 7. The outer cover 21 is a transparent glass shield. Slide grooves are arranged on both sides of the front end of the outer cover 21. The door 22 is installed in the slide grooves at the front end of the outer cover 21. The door 22 can move up and down. When placing specimens and slides, the door 22 moves up and is lifted. When the slide making machine is working, the door 22 moves down and closes.

[0066] The pipette tip box 15, the specimen collection bottle box 16, and the slide box 17 are all arranged on the upper surface of the base 7. The pipette tip box 15 is used to place the pipette tip, the specimen collection bottle is placed in the specimen collection bottle box 16, and the slide is placed in the slide box 17. The slide box 17 is provided with a resistance wire for heating, and the resistance wire is a purchased standard part.

[0067] The switch 18 , the USB interface 19 , and the video monitor 20 are all arranged on the front surface of the base 7 .

[0068] The test tube rack rotation drive mechanism, waste liquid tank, camera and control system are all arranged inside the base 7.

[0069] In the present invention, the X-axis motion mechanism 1, the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, and the second Y-axis motion mechanism 4 constitute a manipulator absorber moving system. The manipulator absorber moving system moves in three directions, that is, it can simultaneously move in the X-axis, Y-axis, and Z-axis. The left-right movement is along the X-axis, the front-back movement is along the Y-axis, and the up-and-down movement is along the Z-axis. The X-axis motion mechanism 1 uses a synchronous toothed belt to transmit power, and the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, and the second Y-axis motion mechanism 4 all use gear racks to transmit power. The straw is installed in the straw socket 203 of the manipulator absorber 204, and the manipulator absorber 204 is moved to the designated position for absorption and smearing by driving the X-axis motion mechanism 1, the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, and the second Y-axis motion mechanism 4.

[0070] Among them, the X-axis motion mechanism 1, the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, the second Y-axis motion mechanism 4, the test tube rack rotation drive mechanism, the switch 18, the USB interface 19, the video monitor 20, and the camera are all electrically connected to the control system.

[0071] The first Y-axis motion mechanism 3 is installed on the left side of the upper surface of the base 7, and the second Y-axis motion mechanism 4 is installed on the right side of the upper surface of the base 7. The first Y-axis motion mechanism 3 and the second Y-axis motion mechanism 4 can slide on the base 7 along the Y-axis direction.

[0072] Two ends of the support frame 5 are respectively fixed on the first Y-axis motion mechanism 3 and the second Y-axis motion mechanism 4 , and two ends of the transverse guide rail 6 are respectively fixed on the support frame 5 .

[0073] The upper end of the X-axis motion mechanism 1 is mounted on the support frame 5 , and the lower end of the X-axis motion mechanism 1 is mounted on the transverse guide rail 6 . The X-axis motion mechanism 1 can slide on the support frame 5 and the transverse guide rail 6 along the X-axis direction.

[0074] The Z-axis motion mechanism 2 is installed on the X-axis motion mechanism 1 , and the Z-axis motion mechanism 2 can slide on the X-axis motion mechanism 1 along the Z-axis direction.

[0075] like Figure 4 As shown, the X-axis motion mechanism 1 mainly includes: an X-axis moving card seat 101, an X-axis moving servo motor 102, an X-axis moving driving gear 103, an X-axis synchronous toothed belt 104, a Z-axis synchronous toothed belt 105, a dovetail groove 106 and a mounting hole 107. The X-axis moving servo motor 102 is connected to the control system. The X-axis moving servo motor 102 is fixed inside the X-axis moving card seat 101, and the X-axis moving driving gear 103 is fixedly connected to the output shaft 108 of the X-axis moving servo motor 102 through a flat key 109. A mounting hole 107 is respectively arranged on both sides of the upper end of the X-axis moving card seat 101, and the support frame 5 extends into the two mounting holes 107 on both sides of the upper end of the X-axis moving card seat 101, and then the X-axis synchronous toothed belt 104 arranged on the support frame 5 and the X-axis moving driving gear 103 are meshed with each other. A mounting hole is also provided on both sides of the lower end of the X-axis moving card seat 101, and the transverse guide rail 6 extends into the mounting holes on both sides of the lower end of the X-axis moving card seat 101.

[0076] Z-axis synchronous toothed belts 105 are respectively arranged on the inner walls on both sides of the front end opening of the X-axis moving card seat 101, and dovetail grooves 106 are respectively arranged on the outer walls on both sides of the front end opening of the X-axis moving card seat 101.

[0077] The X-axis moving servo motor 102 is started to drive the X-axis moving driving gear 103 to rotate, and the entire X-axis moving cassette 101 is driven to move along the X-axis on the supporting frame 5 through the meshing action between the X-axis moving driving gear 103 and the X-axis synchronous toothed belt 104.

[0078] like Figure 6 and Figure 7As shown, the Z-axis motion mechanism 2 mainly includes: a Z-axis moving card holder 201, a Z-axis moving servo motor 202, a straw socket 203, a manipulator suction device 204, a dovetail structure 205, and a Z-axis moving driving gear 206. The Z-axis moving servo motor 202 is connected to the control system. The Z-axis moving servo motor 202 is fixed to the upper end of the front surface of the Z-axis moving card holder 201, and the manipulator suction device 204 is installed in the mounting hole at the lower end of the front surface of the Z-axis moving card holder 201. At the same time, the upper end of the manipulator suction device 204 is fixedly connected to the output shaft 207 of the Z-axis moving servo motor 202, and the straw socket 203 is arranged at the lower end of the manipulator suction device 204. A dovetail structure 205 is respectively arranged on both sides of the rear surface of the Z-axis moving card holder 201. A Z-axis moving driving gear 206 is arranged at the upper end of the rear surface of the Z-axis moving card holder 201. The Z-axis moving driving gear 206 is installed in the lower end opening of the X-axis moving base 101, and the Z-axis moving driving gear 206 is meshed with the Z-axis synchronous toothed belt 105, and the dovetail structure 205 and the dovetail groove 106 are installed in a one-to-one correspondence.

[0079] The Z-axis moving servo motor 202 is started to drive the Z-axis moving driving gear 206 to rotate, and the entire Z-axis moving card holder 201 and the manipulator suction device 204 are driven to move along the Z axis on the X-axis moving card holder 101 through the meshing action between the Z-axis moving driving gear 206 and the Z-axis synchronous toothed belt 105.

[0080] The first Y-axis motion mechanism 3 and the second Y-axis motion mechanism 4 have the same structure and size.

[0081] like Figure 2 and Figure 8 As shown, the first Y-axis motion mechanism 3 mainly includes: a first Y-axis moving card seat 301, a first Y-axis moving servo motor 303, a first Y-axis moving driving gear 302, a first rack 8 and a first linear guide 10. The first Y-axis moving servo motor 303 is connected to the control system. The lower end of the left side of the support frame 5 is fixed on the first Y-axis moving card seat 301. One side of the lower end of the first Y-axis moving card seat 301 is installed on the first linear guide 10, and the other side of the lower end of the first Y-axis moving card seat 301 is set in an open form. The first Y-axis moving servo motor 303 is fixed inside the opening of the first Y-axis moving card seat 301. The first Y-axis moving driving gear 302 is fixedly connected to the output shaft 305 of the first Y-axis moving servo motor 303 through a flat key 304, and the first Y-axis moving driving gear 302 and the first rack 8 are meshed with each other.

[0082] The first Y-axis moving servo motor 303 is started to drive the first Y-axis moving driving gear 302 to rotate, and the entire first Y-axis moving cartridge 301 is driven to move along the Y-axis direction on the first linear guide rail 10 through the meshing action between the first Y-axis moving driving gear 302 and the first rack 8 .

[0083] like Figure 2 and Fig.10 As shown, the second Y-axis motion mechanism 4 mainly includes: a second Y-axis moving card seat 401, a second Y-axis moving servo motor 403, a second Y-axis moving driving gear 402, a second rack 9 and a second linear guide 11. The second Y-axis moving servo motor is connected to the control system. The lower end of the right side of the support frame 5 is fixed on the second Y-axis moving card seat 401. One side of the lower end of the first Y-axis moving card seat 401 is installed on the second linear guide 11, and the other side of the lower end of the second Y-axis moving card seat 401 is set in an open form. The second Y-axis moving servo motor is fixed inside the opening of the second Y-axis moving card seat 401, and the second Y-axis moving driving gear 402 is fixedly connected to the output shaft of the second Y-axis moving servo motor, and the second Y-axis moving driving gear 402 and the second rack 9 are meshed with each other.

[0084] The second Y-axis moving servo motor is started to drive the second Y-axis moving driving gear 402 to rotate, and the entire second Y-axis moving holder 401 is driven to move along the Y-axis direction on the second linear guide rail 11 through the meshing action between the second Y-axis moving driving gear 402 and the second rack 9.

[0085] The first rack 8 and the first linear guide 10 are both fixed to the left side of the upper surface of the base 7 by screws, and the second rack 9 and the second linear guide 11 are both fixed to the right side of the upper surface of the base 7 by screws. The first rack 8 is located outside the first linear guide 10, and the second rack 9 is located outside the second linear guide 11.

[0086] like Figure 3 As shown, the test tube rack rotation drive mechanism mainly includes: a vertical shaft 23, a first ball bearing 24, a driving gear 25, a passive gear 26, a rotation servo motor 27, a second ball bearing 28, a thrust bearing 29, a rotation servo motor fixing seat and a bearing seat.

[0087] The rotary servo motor fixing seat and the bearing seat are both fixed inside the machine base 7.

[0088] The test tube rack 13 is fixed to the upper end of the vertical shaft 23 by screws.

[0089] The thrust bearing 29, the second ball bearing 28, the driving gear 25, and the first ball bearing 24 are sequentially installed at the lower end of the vertical shaft 23 from bottom to top. Among them, the outer diameter of the lower end of the vertical shaft 23 is transitionally matched with the inner hole of the thrust bearing 29, and the lower end face of the thrust bearing 29 contacts the bearing seat inside the machine base 7. The thrust bearing 29 mainly bears the axial force, and the upper end face of the thrust bearing 29 contacts the inner ring of the second ball bearing 28. The inner holes of the second ball bearing 28 and the inner holes of the first ball bearing 24 are transitionally matched with the outer diameter of the vertical shaft 23. The second ball bearing 28 and the first ball bearing 24 rotate together with the vertical shaft 23, and the outer diameters of the second ball bearing 28 and the first ball bearing 24 are fixedly connected with the bearing seat inside the machine base 7. The second ball bearing 28 and the first ball bearing 24 play the role of fixing the vertical shaft 23 and reducing the rotation resistance.

[0090] The driving gear 25 is installed between the second ball bearing 28 and the first ball bearing 24 , and the inner diameter of the driving gear 25 is dynamically matched with the outer diameter of the vertical shaft 23 .

[0091] The rotary servo motor 27 is fixed to the rotary servo motor fixing seat inside the machine base 7 by bolts, and the rotary servo motor 27 is electrically connected to the control system. The driving gear 25 is meshed with the passive gear 26, and the inner hole of the rotary driving gear 25 is tightly matched with the shaft of the rotary servo motor 27. When the rotary servo motor 27 rotates, the driving gear 25 is driven to rotate together through the passive gear 26, and the passive gear 26 meshes and rotates with the driving gear 25, driving the vertical shaft 23 to rotate, and at the same time driving the test tube rack 13 on the vertical shaft 23 to rotate together.

[0092] When the liquid-based cell automatic slide making machine of the present invention is used, work preparation is first performed, cell specimens, glass slides, centrifuge tubes 14, test tubes 30 and other preparations are placed, labeling is started, barcode scanner is used to scan the code, door 22 is closed, switch 18 is turned on, processing mode is selected, and the X-axis motion mechanism 1, Z-axis motion mechanism 2, first Y-axis motion mechanism 3, and second Y-axis motion mechanism 4 work together to move the manipulator absorber 204 to the top of the pipette tip, extract the pipette tip, and then the X-axis motion mechanism 1, Z-axis motion mechanism 2, first Y-axis motion mechanism 3, and second Y-axis motion mechanism 4 are used to move the manipulator absorber 204 to the top of the pipette tip. The robot absorber 204 is moved to the top of the specimen collection bottle by the X-axis motion mechanism 1, the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, and the second Y-axis motion mechanism 4. The robot absorber 204 is moved to the top of the corresponding centrifuge tube 14 to inject the cell specimen. The robot absorber 204 is moved to the top of the waste liquid tank to discard the pipette, and the corresponding pipette is picked up again. The above actions are repeated until all specimens are extracted. The X-axis motion mechanism 1, the Z-axis motion mechanism 2, the first Y-axis motion mechanism 3, and the second Y-axis motion mechanism 4 jointly drive the robot absorber 204 to stop working and return to its original position.

[0093] The test tube rack rotation drive mechanism drives the vertical shaft 23 and the test tube rack 13 to rotate, so that the centrifuge tube 14 is tilted 90°, the cells adhere to the bottom of the tube, the test tube rack 13 stops rotating, the manipulator absorber 204 moves to the top of the pipette to pick up the 1ml pipette, the manipulator absorber 204 moves to the top of the centrifuge tube 14, absorbs the cell fluid in the centrifuge tube 14, the manipulator absorber 204 rises, the manipulator absorber 204 moves to the top of the test tube 30, and injects cells into the corresponding test tube 30. The control system detects the height of the cell fluid to determine the number of cells. After the test is qualified, the manipulator absorber 204 transfers the cells to the slide (the bottom of the slide is heated by a resistance wire to maintain a temperature of 40°), and the process from the centrifuge tube 14 to the test tube 30 to the slide is repeated many times to complete a cycle of film making. During the film making process, the number of cells on the slide is detected by a camera.

[0094] Turn on switch 18 and select a processing mode, wherein there are three processing modes:

[0095] Mode 1: Whole cytology processing.

[0096] Mode 2: Limited cytological processing.

[0097] Mode 3: Tube cytology processing.

[0098] In the present invention, products are added during sampling and centrifugation, thereby forming a fully automated portable liquid cytology processing system, which is an in vitro universal cytology specimen processing instrument. Various cytology specimens are processed using cytology processing reagents and procedures, so that cells adhere to the slide, and after the slide is dried, it can be stained and read by trained professionals.

[0099] In the description of the present invention, it is necessary to understand that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the protection content of the present invention.

[0100] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Liquid-based cell automatic slide making machine, including: A machine base with a hollow interior, an outer cover fixed on the machine base, and a door installed in a front end slide groove of the outer cover, wherein a through hole is provided at the center of the upper surface of the machine base, and the machine base is characterized in that it also includes: A pipette tip box, a specimen collection bottle box, and a glass slide box are all arranged on the upper surface of the machine base, wherein the pipette tip box is used to place the pipette tip, the specimen collection bottle box is used to place the specimen collection bottle, and the glass slide box is used to place the glass slide; A first Y-axis motion mechanism and a second Y-axis motion mechanism are respectively mounted on the left and right sides of the upper surface of the machine base; A supporting frame, both ends of which are respectively fixed to the first Y-axis motion mechanism and the second Y-axis motion mechanism; An X-axis motion mechanism mounted on a support frame; A Z-axis motion mechanism mounted on an X-axis motion mechanism; A test tube rack rotation drive mechanism installed inside the machine base; A test tube rack fixed to the upper end of the test tube rack rotation drive mechanism, the test tube rack is located in the central through hole of the machine base, and the outer ring and inner ring of the test tube rack are respectively provided with a plurality of evenly distributed card slots; A centrifuge tube installed in the outer ring slot of the test tube rack; The test tube is installed in the inner ring groove of the test tube rack; The first Y-axis motion mechanism comprises: a first Y-axis moving card seat, a first Y-axis moving servo motor, a first Y-axis moving driving gear, a first rack and a first linear guide rail; The first rack and the first linear guide are both fixed to the left side of the upper surface of the machine base, and the first rack is located outside the first linear guide; the lower end of the left side of the support frame is fixed to the first Y-axis movable card seat; one side of the lower end of the first Y-axis movable card seat is installed on the first linear guide, and the other side of the lower end of the first Y-axis movable card seat is set in an open form, the first Y-axis movable servo motor is fixed inside the opening of the first Y-axis movable card seat, the first Y-axis movable servo motor is electrically connected to the control system, the first Y-axis movable drive gear is fixedly connected to the output shaft of the first Y-axis movable servo motor through a flat key, and the first Y-axis movable drive gear and the first rack are meshed with each other; The first Y-axis moving servo motor is started to drive the first Y-axis moving driving gear to rotate, and the entire first Y-axis moving card seat is driven to move along the Y-axis direction on the first linear guide rail through the meshing action between the first Y-axis moving driving gear and the first rack; The second Y-axis motion mechanism comprises: a second Y-axis moving card seat, a second Y-axis moving servo motor, a second Y-axis moving driving gear, a second rack and a second linear guide; the second rack and the second linear guide are both fixed to the right side of the upper surface of the machine base, and the second rack is located outside the second linear guide; the lower end of the right side of the support frame is fixed to the second Y-axis moving card seat; one side of the lower end of the first Y-axis moving card seat is installed on the second linear guide, and the other side of the lower end of the second Y-axis moving card seat is set in an open form, the second Y-axis moving servo motor is fixed inside the opening of the second Y-axis moving card seat, the second Y-axis moving servo motor is electrically connected to the control system, the second Y-axis moving driving gear is fixedly connected to the output shaft of the second Y-axis moving servo motor, and the second Y-axis moving driving gear and the second rack are meshed with each other; The second Y-axis moving servo motor is started to drive the second Y-axis moving driving gear to rotate, and the entire second Y-axis moving card seat is driven to move along the Y-axis direction on the second linear guide rail through the meshing action between the second Y-axis moving driving gear and the second rack.

2. The liquid-based cell automatic slide making machine according to claim 1, characterized in that: Also includes: Switches, USB ports and video monitors are all located on the front surface of the base; The waste liquid tank, camera and control system are all set inside the base; The X-axis motion mechanism, the Z-axis motion mechanism, the first Y-axis motion mechanism, the second Y-axis motion mechanism, the switch, the USB interface, the video monitor, and the camera are all electrically connected to the control system.

3. The liquid-based cell automatic slide making machine according to claim 2, characterized in that: Also includes: The two ends are respectively fixed to the transverse guide rail on the support frame, and the lower end of the X-axis motion mechanism is installed on the transverse guide rail.

4. The liquid-based cell automatic slide making machine according to claim 3, characterized in that: The X-axis motion mechanism comprises: An X-axis movable card seat, wherein mounting holes are respectively arranged on both sides of the upper end of the X-axis movable card seat, and the support frame extends into the two mounting holes on both sides of the upper end of the X-axis movable card seat; mounting holes are respectively arranged on both sides of the lower end of the X-axis movable card seat, and the transverse guide rail extends into the mounting holes on both sides of the lower end of the X-axis movable card seat; Z-axis synchronous toothed belts are respectively arranged on the inner walls of both sides of the front opening of the X-axis moving card seat; Dovetail grooves are respectively arranged on the outer walls of both sides of the front opening of the X-axis moving card seat; An X-axis moving servo motor fixed inside the X-axis moving card holder and electrically connected to the control system; An X-axis moving driving gear fixedly connected to the output shaft of the X-axis moving servo motor via a flat key; An X-axis synchronous toothed belt disposed on the support frame, wherein the X-axis synchronous toothed belt is meshed with the X-axis moving drive gear; The X-axis moving servo motor is started to drive the X-axis moving driving gear to rotate, and the entire X-axis moving card seat is driven to move along the X-axis on the supporting frame through the meshing action between the X-axis moving driving gear and the X-axis synchronous toothed belt.

5. The liquid-based cell automatic slide making machine according to claim 4, characterized in that: The Z-axis motion mechanism comprises: Z-axis moving card holder; A Z-axis moving servo motor fixed to the upper end of the front surface of the Z-axis moving card holder and electrically connected to the control system; A manipulator absorber is installed in the mounting hole at the lower end of the front surface of the Z-axis moving card seat, and the upper end of the manipulator absorber is fixedly connected to the output shaft of the Z-axis moving servo motor; A straw socket is arranged at the lower end of the manipulator adsorber, and a straw is installed in the straw socket; Dovetail structures are respectively arranged on both sides of the rear surface of the Z-axis moving card seat, and the dovetail structures are installed in a one-to-one correspondence with the dovetail grooves; A Z-axis moving drive gear is arranged at the upper end of the rear surface of the Z-axis moving card seat, and the Z-axis moving drive gear is installed in the lower end opening of the X-axis moving card seat, and the Z-axis moving drive gear is meshed with the Z-axis synchronous toothed belt; The Z-axis moving servo motor is started to drive the Z-axis moving driving gear to rotate, and the entire Z-axis moving card seat and the manipulator absorber are driven to move along the Z axis on the X-axis moving card seat through the meshing action between the Z-axis moving driving gear and the Z-axis synchronous toothed belt.

6. The liquid-based cell automatic slide making machine according to claim 2, characterized in that: The test tube rack rotation drive mechanism comprises: a vertical shaft, a first ball bearing, a driving gear, a passive gear, a rotation servo motor, a second ball bearing, a thrust bearing, a rotation servo motor fixing seat and a bearing seat; The rotating servo motor fixing seat and the bearing seat are both fixed inside the machine base; the test tube rack is fixed on the upper end of the vertical shaft; the thrust bearing, the second ball bearing, the driving gear, and the first ball bearing are sequentially installed on the lower end of the vertical shaft from bottom to top; the outer diameter of the lower end of the vertical shaft is transitionally matched with the inner hole of the thrust bearing, the lower end face of the thrust bearing contacts with the bearing seat inside the machine base, the thrust bearing bears the axial force, the upper end face of the thrust bearing contacts with the inner ring of the second ball bearing, the inner hole of the second ball bearing and the inner hole of the first ball bearing are both transitionally matched with the outer diameter of the vertical shaft, the second ball bearing and the first ball bearing both rotate together with the vertical shaft, the outer diameter of the second ball bearing and the first ball bearing The outer diameters of the bearings are fixedly connected to the bearing seats inside the machine base; the driving gear is installed between the second ball bearing and the first ball bearing, and the inner diameter of the driving gear is dynamically matched with the outer diameter of the vertical shaft; the rotary servo motor is fixed on the rotary servo motor fixing seat inside the machine base, and the rotary servo motor is electrically connected to the control system; the driving gear is meshed with the passive gear, and the inner hole of the rotary driving gear is tightly matched with the rotary servo motor shaft; when the rotary servo motor rotates, the passive gear drives the driving gear to rotate together, and the passive gear meshes and rotates with the driving gear, driving the vertical shaft to rotate, and at the same time drives the test tube rack on the vertical shaft to rotate together.

7. The liquid-based cell automatic slide making machine according to claim 1, characterized in that: A resistance wire is arranged in the slide box.

8. The liquid-based cell automatic slide making machine according to claim 1, characterized in that: It also includes a ring arranged on the outer circle of the central through hole of the machine base.

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