Electrophoresis gel plate and electrophoresis gel preparation method

Abstract

The present invention provides an electrophoresis gel plate and an electrophoresis gel preparation method. The electrophoresis gel plate uses a spacer to isolate the upper liquid surface of the gel into a plurality of sample loading grooves spaced apart from each other in the horizontal direction. Gel production no longer requires an electrophoresis comb, which reduces components, reduces material costs, saves the action of inserting the comb, and reduces the gel making time; since the side walls of the sample loading grooves are formed by the spacers and the plate body wall, the structure of the sample loading grooves is stable and cross contamination between samples will not occur; the sample loading grooves are between the spacers, and the contours of the sample loading grooves are clearly visible, and no sample loading hole position indicator lines or sample loading hole indicators are required, which facilitates the sample loading operation of the experimenter. The electrophoresis gel preparation method uses a balance liquid to effectively reduce gel leakage caused by centrifugation, and automatically layers are separated by centrifugation without the need for layered solidification, and can be produced continuously, which greatly improves production efficiency; bubbles in the gel can be discharged more easily and quickly, and the colloid and the spacers are more tightly combined and fully solidified, eliminating internal leakage.

Description

Technical Field

[0001] The invention relates to the technical field of bio-electrophoresis, in particular to an electrophoresis gel plate and a method for preparing an electrophoresis gel. Background Art

[0002] Electrophoresis is an important and indispensable analytical method in molecular biology research, used to separate and identify protein, DNA, and RNA molecular mixtures. Electrophoresis is the movement of charged particles in an electric field. Different substances have different movement speeds in an electric field due to their different charges and molecular weights. Based on this feature, electrophoresis can be used to conduct qualitative or quantitative analysis of different substances, or to analyze the components of a certain mixture or extract and prepare a single component, which is extremely important in clinical testing or experimental research.

[0003] In the electrophoresis experiment, gel needs to be prepared first. In the Chinese patent document with the publication number CN201222053Y, a disposable electrophoresis gel plate is disclosed. Figure 1 The figure shows the mainstream electrophoresis gel plate in the prior art, which includes a cover plate 91, a bottom plate 93 and a sample adding grid 92. When in use, the cover plate 91 and the bottom plate 93 are first buckled together, and liquid gel is injected into the cavity, and then the sample adding grid 92 is inserted into the upper opening. After the gel solidifies, the sample adding grid 92 is taken out before the experiment, and the grid teeth 921 of the sample adding grid leave a sample adding groove in the solidified gel for adding the experimental sample. The above-mentioned existing structure of the gel making plate has the following disadvantages: (1) When preparing the gel, unstable bubbles are easily generated at the insertion point of the sample adding grid. After the gel is formed, the sample adding groove formed after the sample adding grid is pulled out has uneven gel wall thickness, and the gel wall is easy to collapse, forming residual glue in the groove, and even causing the adjacent sample adding grooves to penetrate, causing mutual contamination between samples; (2) In order to reduce the generation of bubbles, it is necessary to manually confirm whether there are bubbles when inserting the sample adding grid. If there are bubbles, it needs to be re-inserted, which consumes high labor costs and is not convenient for industrial mass production; After the gel is solidified, you must be very careful when pulling out the sample loading grid. If you pull it out quickly or tilted, it will also cause damage to the wall of the sample loading groove; (3) Since there is a strip-shaped conductive port at the bottom of the electrophoresis gel plate, if the sealing of the strip-shaped conductive port is not reliable, or there is a gap between the cover plate and the bottom plate, it is easy to leak glue, which will cause the liquid level on the gel to drop. The sample loading groove created by the grid teeth will become smaller and cannot accommodate enough experimental samples; (4) Since the gel is a transparent body, it is not easy for the experimenter to see the specific position of the sample loading groove in the gel, which is inconvenient for adding samples. Figure 1 In some embodiments, the sample adding hole position indicator line 931 is printed on the rubber plate, and a sample adding hole indicator is also used, which will increase the cost of the rubber plate. Summary of the invention

[0004] In order to overcome the above-mentioned deficiencies of the prior art, the object of the present invention is to provide an electrophoresis gel plate and a method for preparing an electrophoresis gel.

[0005] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention to solve its technical problems is: an electrophoresis gel plate, including a first plate body and a second plate body, the first plate body and the second plate body are overlapped and lean against each other to form a gel-making cavity, the upper part of the first plate body and / or the second plate body has a plurality of parallelly arranged partitions, the upper liquid level of the gel poured into the gel-making cavity is higher than the lower bottom surface of the partition, and the adjacent partitions isolate the space above the gel liquid level into a sample addition groove for adding experimental samples, and each partition is also provided with a conductive groove.

[0006] By adopting the technical solution of the present invention, gel production no longer requires a sample loading grid (commonly known as an electrophoresis comb), which reduces components, reduces material costs, saves the comb insertion action, and reduces gel production time; because the side walls of the sample loading groove are formed by the partition bars and the plate body wall, even if bubbles are generated on the upper part of the colloid or even if a small amount of gel leakage occurs, the structure of the sample loading groove remains stable and cross contamination between samples will not occur; viewed from the front side of the electrophoresis gel plate, the sample loading groove is between the partition bars, the contour of the sample loading groove is clearly visible, and no sample loading hole position indicator line or sample loading hole indicator is required, which facilitates the sample loading operation of the experimenter.

[0007] Furthermore, after the gel solution is poured into the gel-making cavity, during electrophoresis, in the spacer area, the conductive groove forms a channel connecting the buffer solution and the gel.

[0008] By adopting the above preferred solution, isolation electric field lines are formed along the straight line area of ​​the conductive groove to isolate the electrophoresis bands of adjacent experimental samples, so that the electrophoresis spectrum is clearer.

[0009] Furthermore, a conductive strip is embedded in the conductive groove. During electrophoresis, the conductive strip forms an electrical conduction path between the buffer solution and the gel in the strip area.

[0010] By adopting the above preferred solution, conductive strips are embedded in the conductive groove to form isolated electric field lines, so that the electrophoresis pattern is clearer, the conductive groove can be made narrower, and the size of the electrophoresis gel plate can be reduced.

[0011] Furthermore, the partition bar is arranged at the upper part of one side of the second plate body, and the second plate body is provided with a connecting bonding surface on the left part, the lower part and the right part of the side where the partition bar is located and is in the same plane as the side of the partition bar, and one side of the first plate body is attached to the bonding surface and the side of the partition bar; the top of the first plate body has a buffer solution upper immersion inlet lower than the top surface of the second plate body, and the second plate body has a buffer solution lower immersion inlet arranged horizontally at the bottom position of the gel-making cavity; during electrophoresis, the buffer solution immerses the buffer solution upper immersion inlet on the top of the first plate body and conducts with one electrode, and the buffer solution lower immersion inlet at the bottom of the second plate body is immersed in the buffer solution and conducts with another electrode, thereby forming an electric field at both ends of the gel.

[0012] Furthermore, the outer edge of the fitting surface of the second plate body is also provided with a limiting boss matching the outer contour of the first plate body.

[0013] The above preferred solution is adopted to facilitate the assembly of the first plate body and the second plate body.

[0014] Furthermore, four walls of each of the sample loading grooves are integrally formed on the first plate body or the second plate body.

[0015] Furthermore, the first plate body includes a first main wall body and a first auxiliary wall body, the partition bar is arranged between the first main wall body and the first auxiliary wall body and the three are formed as one piece, the first auxiliary wall body is located in the area opposite to the partition bar at the upper part of the first main wall body, and the second plate body overlaps with the lower middle part of the first main wall body and the first auxiliary wall body; the top surface of the first main wall body has an upper buffer solution immersion inlet lower than the top surface of the first auxiliary wall body, and the second plate body has a horizontally laterally arranged lower buffer solution immersion inlet corresponding to the bottom position of the gel-making cavity.

[0016] Furthermore, the second plate body includes a second main wall body and a second auxiliary wall body, the partition bar is arranged between the second main wall body and the second auxiliary wall body and the three are formed as one piece, the second auxiliary wall body is located in the area opposite to the partition bar at the upper part of the second main wall body, and the first plate body overlaps with the lower middle part of the second main wall body and the second auxiliary wall body; the top surface of the first plate body and the top surface of the second auxiliary wall body are flush and the top surfaces of both have an upper buffer immersion inlet lower than the top surface of the second main wall body, and the second main wall body has a horizontally laterally arranged lower buffer immersion inlet corresponding to the bottom position of the glue making cavity.

[0017] By adopting the above preferred solution, the four walls of the sample adding groove are integrally formed, which effectively prevents leakage and avoids liquid contamination between samples; the first plate body and the second plate body are more convenient to mass produce using injection molds, saving costs.

[0018] A method for preparing an electrophoresis gel comprises the following steps:

[0019] S1, plate stacking step: firstly stack a plurality of electrophoresis gel plates against each other and arrange them vertically in a plastic bag, then put them together with the plastic bag into a container, and press the electrophoresis gel plate combination tightly;

[0020] S2, gel adding step: injecting gel solution into the gel making cavity of each electrophoresis gel plate;

[0021] S3, adding balancing solution step: adding balancing solution into the plastic bag;

[0022] S4, centrifugation step: placing the container on a centrifuge for centrifugation;

[0023] S5, solidification and packaging step: after the colloid solidifies, take out the plastic bag and the electrophoresis gel sheet, and package them together for shipment.

[0024] By adopting the above technical solution, a balance liquid is immersed in the joint gap of the rubber plate, and the liquid pressure is used to resist the centrifugal force, which can effectively reduce the leakage of glue caused by centrifugation and ensure the stable centrifugation. Glues of various concentrations can be poured in sequence or simultaneously, and automatically layered by centrifugation, without the need for layered solidification, and can be produced continuously, which greatly improves the production efficiency; the bubbles in the glue are more easily and quickly discharged under the action of centrifugation, so that the surface of the gel remains consistent and flat, the colloid and the partition are more tightly combined, and the contact between the gel and the partition can be fully solidified, eliminating internal leakage, which can effectively prevent samples from contaminating each other. Plastic bags are directly used as inner packaging, and the production batch number is printed. The liquid will not flow out, keeping the production environment clean.

[0025] Furthermore, in S2, in the glue adding step, glue is injected using an AB mixing tube, the glue solution in tube A contains an accelerator TEMED, and the solution in tube B contains a catalyst ammonium persulfate.

[0026] By adopting the above preferred solution, the glue in tubes A and B begins to polymerize and solidify only in the mixing tube, and there is no need to premix a large amount of gel in advance, thus saving the waste of raw materials.

[0027] Furthermore, when preparing gradient glue, in S2, the glue adding step, multiple glue adding positions are arranged on the production line, each glue adding position is used to inject glue of different concentrations, and the container moves from the high concentration glue adding position to the low concentration glue adding position to add glue.

[0028] By adopting the above preferred solution, no strict production process and conditions are required. Glues of different concentrations are poured in sequence and finally centrifuged and automatically stratified, so that gradient glues with gradually changing concentrations can be continuously produced. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] Figure 1 It is a structural schematic diagram of the prior art;

[0031] Figure 2 It is a three-dimensional schematic diagram of a first embodiment of the electrophoresis gel plate of the present invention;

[0032] Figure 3 is with Figure 2 Exploded view of the corresponding electrophoresis gel plate;

[0033] Figure 4 yes Figure 2 A partial enlarged view of the middle A;

[0034] Figure 5 is with Figure 2 The front view of the corresponding electrophoresis gel plate;

[0035] Figure 6 yes Figure 5 Middle BB section view;

[0036] Figure 7 It is a schematic diagram of the principle of gel electrophoresis made by using the electrophoresis gel plate of the present invention;

[0037] Figure 8 It is a front perspective schematic diagram of a second embodiment of the electrophoresis gel plate of the present invention;

[0038] Fig. 9 is with Figure 8 A three-dimensional schematic diagram of the back side of the corresponding electrophoresis gel plate;

[0039] Fig.10 is with Fig. 9 Exploded view of the corresponding electrophoresis gel plate;

[0040] Fig.11 yes Fig.10 Middle CC section view;

[0041] Fig.12 It is a structural schematic diagram of a method of embedding a conductive strip in the middle of a spacer bar;

[0042] Fig.13 is a flow chart of the method for preparing an electrophoresis gel;

[0043] Fig.14 It is the structural principle diagram of the plate stacking step;

[0044] Fig.15 It is a schematic diagram of the structure of the AB mixing tube;

[0045] Fig.16 It is a schematic diagram of the glue adding principle for preparing gradient glue;

[0046] Fig.17 is a schematic diagram of the obtained gradient gel concentration change;

[0047] Fig.18 is a three-dimensional schematic diagram of a third embodiment of the electrophoresis gel plate of the present invention;

[0048] Fig.19 is with Fig.18 Exploded view of the corresponding electrophoresis gel plate;

[0049] Fig. 20 is with Fig.18Schematic diagram of two corresponding electrophoresis gel plates stacked together.

[0050] The numbers and letters in the figure represent the names of the corresponding parts:

[0051] 10-electrophoresis gel plate; 11-first plate body; 111-buffer upper immersion inlet; 112-first main wall body; 113-first auxiliary wall body; 12-second plate body; 121-second main wall body; 122-second auxiliary wall body; 123-fitting surface; 124-limiting boss; 125-buffer lower immersion inlet; 13-spacer; 131-conductive groove; 132-conductive strip; 133-lower bottom surface of spacer; 134-spacer wall body; 14-sample loading groove; 15-gel cavity; 16-easy-pry groove; 17-positioning column; 18-chamfer; 30-gel; 301-upper liquid surface of gel; 40-experimental sample; 50-electrophoresis tank; 60-buffer; 70-AB mixing tube; 701-A tube; 702-B tube; 703-mixing tube; 81-container; 82-plastic bag; 83-pressing mechanism; 831-fixed inclined plate; 832-tightening plug plate; 833-anti-dropping pin; 91-cover plate; 92-sample adding grid mold; 921-grid mold teeth; 93-bottom plate; 931-sample adding hole position indicator line. DETAILED DESCRIPTION

[0052] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] like Figure 2-6 As shown, an electrophoresis gel plate 10 includes a first plate body 11 and a second plate body 12, the first plate body 11 and the second plate body 12 are stacked and form a gel-making cavity 15, and the upper part of the second plate body 12 has a plurality of parallel spacers 13, the upper liquid level 301 of the gel poured into the gel-making cavity 15 is higher than the lower bottom surface 133 of the spacers, and the adjacent spacers 13 isolate the space above the gel liquid level into a sample addition groove 14 for adding experimental samples, and each spacer 13 is also provided with a conductive groove 131. In order to explain the spacers 13 more clearly and avoid misunderstanding, Figure 4 A partial enlarged view is shown. Figure 4 Only two spacers 13 are shown in the figure. It should be emphasized that the spacers 13 mentioned here are a combination of a conductive groove 131 and two side wall bodies 134. The conductive groove 131 exists to form an isolation electric field line between the electrophoretic electric fields of two adjacent samples. Figure 4In the embodiment, a combination of a conductive slot 131 and the partition wall bodies 134 on both sides thereof is defined as a partition bar 13 .

[0054] The beneficial effects of adopting the above technical solution are: gel production no longer requires a sample adding grid mold (commonly known as an electrophoresis comb), which reduces parts, reduces material costs, saves the combing action, and reduces the gel making time; because the side walls of the sample adding groove are formed by partitions and the plate wall, even if bubbles are generated on the upper part of the colloid or even if a small amount of gel leakage occurs, the structure of the sample adding groove remains stable; viewed from the front side of the electrophoresis gel plate, the sample adding groove is between the partitions, and the outline of the sample adding groove is clearly visible, which is convenient for the experimenter to perform sample adding operations.

[0055] In other embodiments of the present invention, the first plate body and the second plate body of the electrophoretic gel plate can be clamped together by external force or a clamping mechanism, can be fastened by a locking element, can be bonded together by gluing, or can be bonded together by heat welding.

[0056] like Figure 3 , 4 As shown, in other embodiments of the present invention, after the gel-making cavity 15 is filled with gel solution, during electrophoresis, the conductive groove 131 forms a channel connecting the buffer solution and the gel in the area of ​​the spacer 13. The beneficial effect of adopting the above technical solution is that an isolation electric field line is formed along the straight area of ​​the conductive groove to isolate the electrophoresis bands of adjacent experimental samples, making the electrophoresis spectrum clearer.

[0057] like Fig.12 As shown, in some other embodiments of the present invention, a conductive strip 132 is embedded in the conductive groove. During electrophoresis, the conductive strip 132 forms an electrical conduction path between the buffer and the gel in the area of ​​the spacer strip 13. The beneficial effects of the above technical solution are: the conductive strip is embedded in the conductive groove to form an isolated electric field line, so that the electrophoresis pattern is clearer, the conductive groove can be made narrower, and the size of the electrophoresis gel plate is reduced.

[0058] like Figure 2-4As shown, in some other embodiments of the present invention, the spacer 13 is arranged at the upper part of one side of the second plate body 12, and the second plate body 12 is provided with a connecting bonding surface 123 on the left side, the lower side and the right side of the side where the spacer is located, and the bonding surface 123 is in the same plane as the side of the spacer. One side of the first plate body 11 is attached to the bonding surface 123 and the side of the spacer 13; the top of the first plate body 11 has a buffer upper immersion inlet 111 lower than the top surface of the second plate body 12, and the second plate body 12 has a buffer lower immersion inlet 125 arranged horizontally and laterally at the bottom position of the gel-making cavity; during electrophoresis, the buffer immerses the buffer upper immersion inlet 111 at the top of the first plate body and conducts with one electrode, and the buffer lower immersion inlet 125 at the bottom of the second plate body is immersed in the buffer and conducts with another electrode, forming an electric field at both ends of the gel. The outer edge of the bonding surface of the second plate body 12 is also provided with a limiting boss 124 matching the outer contour of the first plate body. When preparing the gel, the buffer lower immersion inlet 125 can be sealed by a sealing film, or two electrophoresis gel plates can be stacked and the buffer lower immersion inlet 125 can be sealed by the side walls of the adjacent electrophoresis gel plates. Figure 6 , 7 As shown, gel is first poured into the gel-making cavity 15 between the first plate body and the second plate body to form a gel 30; before electrophoresis, the experimental sample 40 is placed in the sample loading groove 14 on the upper part of the gel; during electrophoresis, the electrophoresis gel plate after gel pouring and sample placement is placed as a whole into the electrophoresis tank 50, and the buffer solution 60 on one side of the electrophoresis tank 50 is immersed in the buffer solution upper immersion inlet on the top of the first plate body and is connected to an electrode, and the buffer solution lower immersion inlet at the bottom of the second plate body is immersed in the buffer solution on the other side of the electrophoresis tank 50 and is connected to another electrode, so that an electric field is formed at both ends of the gel.

[0059] In some other embodiments of the present invention, the first plate body and the second plate body are made of transparent material.

[0060] like Figure 8-11 As shown, in some other embodiments of the present invention, the four walls of each sample loading groove 14 are integrally formed on the first plate body 11. The first plate body 11 includes a first main wall body 112 and a first auxiliary wall body 113, the first auxiliary wall body 113 is located at the upper part opposite to the first plate body 11, the partition bar 13 is arranged between the first main wall body 112 and the first auxiliary wall body 113, and the three are integrally formed; the top surface of the first main wall body 112 has a buffer upper immersion inlet lower than the top surface of the first auxiliary wall body 113, the top of the second plate body 12 is against the bottom surface of the first auxiliary wall body 113, and the second plate body 12 has a buffer lower immersion inlet 125 arranged horizontally and laterally at the bottom position of the glue making cavity. The beneficial effects of adopting the above technical scheme are: the four walls of the sample loading groove are integrally formed, which effectively prevents liquid leakage and avoids liquid contamination between samples; the first plate body and the second plate body are more convenient to mass produce using injection molds, saving costs.

[0061] like Fig.10As shown, in some other embodiments of the present invention, the lower end of the partition bar 13 of the first plate body has a downwardly extending portion that exceeds the lower end surface of the first auxiliary wall body, and the upper portion of the inner side surface of the second plate body 12 abuts against the side surface of the downwardly extending portion of the partition bar, so that the downwardly extending portion of the partition bar can form an effective support for the second plate body to prevent deformation of the second plate body.

[0062] like Figure 18-20 As shown, in some other embodiments of the present invention, the second plate body 12 includes a second main wall body 121 and a second auxiliary wall body 122, the partition bar 13 is arranged between the second main wall body 121 and the second auxiliary wall body 122, and the three are formed as one piece, the second auxiliary wall body 122 is located in the area opposite to the partition bar at the upper part of the second main wall body 121, and the first plate body 11 overlaps with the middle and lower part of the second main wall body 121 and the second auxiliary wall body 122; the top surface of the first plate body 11 and the top surface of the second auxiliary wall body 122 are flush, and the top surfaces of both have a buffer upper immersion inlet 111 lower than the top surface of the second main wall body 121, and the second main wall body 121 has a horizontally arranged buffer lower immersion inlet 125 corresponding to the bottom position of the glue making cavity. In order to ensure the positional stability of the first plate body 11 and the second plate body 12 overlapping, a positioning column 17 is provided on the second plate body 12, and a positioning hole matching the positioning column 17 is provided on the first plate body 11. In order to facilitate prying apart the first plate and the second plate to take out the experimental colloid after the electrophoresis is completed, there is an easy-pry groove 16 between the first plate and the second plate on the outer side of the joint edge for easy prying. In order to prevent multiple electrophoresis gel plates from being adsorbed to each other after gel injection and difficult to separate, chamfers 18 are provided on the top of the first plate and the second plate to form a gap for easy separation.

[0063] like Fig.13 As shown, a method for preparing an electrophoresis gel comprises the following steps:

[0064] S1, plate stacking step: firstly stack a plurality of electrophoresis gel plates against each other and arrange them vertically in a plastic bag, then put them together with the plastic bag into a container, and press the electrophoresis gel plate combination tightly;

[0065] S2, gel adding step: injecting gel solution into the gel making cavity of each electrophoresis gel plate;

[0066] S3, adding balancing solution step: adding balancing solution into the plastic bag;

[0067] S4, centrifugation step: placing the container on a centrifuge for centrifugation;

[0068] S5, solidification and packaging step: after the colloid solidifies, take out the plastic bag and electrophoresis gel sheet, and package for shipment.

[0069] By adopting the above preferred scheme, gel solutions of various concentrations can be poured in sequentially or simultaneously, and automatically stratified by centrifugation without stratification and solidification, and can be produced continuously, which greatly improves production efficiency; bubbles in the gel solution can be discharged more easily and quickly under the action of centrifugation, so that the surface of the gel is consistent and flat, the colloid and the spacer are more tightly bonded, and the contact between the gel and the spacer can be fully solidified, eliminating internal leakage, and effectively preventing samples from contaminating each other; the amount of balancing liquid added should preferably not exceed the buffer solution immersion inlet of the gel plate, and the balancing liquid is used to immerse the joint gap of the gel plate to resist the centrifugal force with liquid pressure, which can effectively reduce gel leakage caused by centrifugation and ensure stable centrifugation.

[0070] In S1, the specific structure of the electrophoresis gel sheet assembly is not limited in the stacking step, and only needs to play the role of clamping the electrophoresis gel sheet, such as a rubber band with elastic force, or a plastic bag itself has a contraction force that can clamp multiple electrophoresis gel sheets. Fig.14 A clamping mechanism 83 is shown in the figure, which includes a fixed inclined plate 831 fixed inside the container. After the electrophoresis gel sheet is discharged, a wedge-type clamping plate 832 is inserted and then locked by an anti-dropping pin 833.

[0071] The specific composition of the balancing solution added to the plastic bag is not limited, and it can be deionized water or electrophoresis buffer.

[0072] The specific components of the gel of the present invention are not limited and can be obtained from the prior art. For example, polyacrylamide gel is usually made of water, 30% Acr-Bis, Tris, and SDS. The polymerization methods include chemical polymerization using ammonium persulfate / tetramethylethylenediamine and photopolymerization using riboflavin / tetramethylethylenediamine. Since the chemical polymerization method of ammonium sulfate / tetramethylethylenediamine has good stability, it has become the most widely used polymerization method. However, after the two are combined, the gel will solidify quickly, which is easy to cause waste of raw materials. Fig.15 AB mixing tube 70 is shown, which includes tube A 701, tube B 702 and mixing tube 703. Colloid and tetramethylethylenediamine (TEMED) can be poured into tube A 701, and ammonium persulfate (AP) solution can be poured into tube B. With the above preferred solution, the gel in tubes A and B begins to polymerize and solidify only when it enters the mixing tube, and there is no need to premix a large amount of gel in advance, thus saving the waste of raw materials.

[0073] In other embodiments of the present invention, in order to prepare gradient gel more conveniently and quickly, Fig.16As shown in , multiple glue adding positions are arranged on the production line, each of which is used to inject glue of different concentrations, and the container is moved from the high concentration glue adding position to the low concentration glue adding position to add glue. After the glue adding is completed, the glues of different concentrations can be separated into layers by centrifugation, and at the same time, a transition concentration zone is naturally generated between adjacent concentration colloids. According to the requirements of the gradual change of the gel concentration gradient, a reasonable number of glue adding positions and the concentration gradient of the glue injected into the adjacent glue adding positions can be selected. There is no need for strict production processes and conditions. Glues of different concentrations are injected in sequence, and finally centrifuged to automatically separate them, and continuous production can be achieved. Fig.17 Gradient gel with varying concentrations as shown.

[0074] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable ordinary technicians in the field to understand the content of the present invention and implement it. They cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. An electrophoresis gel sheet, characterized in that: It comprises a first plate body and a second plate body, the first plate body and the second plate body are overlapped and form a gel-making cavity, the upper part of the first plate body or / and the second plate body is provided with a plurality of parallel spacers, the upper liquid level of the gel poured into the gel-making cavity is higher than the lower bottom surface of the spacers, the adjacent spacers isolate the space above the gel liquid level into a sample addition groove for adding experimental samples, and each spacer is also provided with a conductive groove; during electrophoresis, in the spacer area, the conductive groove constitutes a channel connecting the buffer solution and the gel; the first plate body and the second plate body are made of transparent material; The partition bar is arranged at the upper part of one side of the second plate body, and the second plate body is provided with a connecting bonding surface which is in the same plane as the side of the partition bar at the left side, the lower side and the right side of the side where the partition bar is located, and one side of the first plate body is attached to the bonding surface and the side of the partition bar; the top of the first plate body has a buffer solution upper immersion inlet which is lower than the top surface of the second plate body, and the second plate body has a buffer solution lower immersion inlet which is horizontally arranged at the side corresponding to the bottom position of the gel-making cavity; Alternatively, the four walls of each of the sample loading grooves are integrally formed on the first plate body or the second plate body, the first plate body includes a first main wall body and a first auxiliary wall body, the partition bar is arranged between the first main wall body and the first auxiliary wall body, and the three are integrally formed, the first auxiliary wall body is located in the area opposite to the partition bar at the upper part of the first main wall body, and the second plate body overlaps with the middle and lower part of the first main wall body and the first auxiliary wall body; the top surface of the first main wall body has a buffer solution upper immersion inlet lower than the top surface of the first auxiliary wall body, and the second plate body has a buffer solution lower immersion inlet arranged horizontally and laterally corresponding to the bottom position of the gel-making cavity; Alternatively, the four walls of each of the sample loading grooves are integrally formed on the first plate body or the second plate body, the second plate body includes a second main wall body and a second auxiliary wall body, the partition bar is arranged between the second main wall body and the second auxiliary wall body and the three are integrally formed, the second auxiliary wall body is located in the area opposite to the partition bar at the upper part of the second main wall body, the first plate body overlaps with the middle and lower part of the second main wall body and the second auxiliary wall body; the top surface of the first plate body and the top surface of the second auxiliary wall body are flush and the top surfaces of both have a buffer solution upper immersion inlet lower than the top surface of the second main wall body, and the second main wall body has a horizontally laterally arranged buffer solution lower immersion inlet corresponding to the bottom position of the gel-making cavity.

2. The electrophoresis gel sheet according to claim 1, characterized in that: The outer edge of the fitting surface of the second plate body is also provided with a limiting boss matching the outer contour of the first plate body.

3. A method for preparing an electrophoresis gel, characterized in that: The steps include: S1, a stacking step: firstly stacking a plurality of electrophoresis gel sheets as claimed in claim 1 against each other and arranging them vertically in a plastic bag, then placing them together with the plastic bag into a container, and pressing the electrophoresis gel sheet assembly; S2, gel adding step: injecting gel solution into the gel making cavity of each electrophoresis gel plate; S3, adding balancing solution step: adding balancing solution into the plastic bag; S4, centrifugation step: placing the container on a centrifuge for centrifugation; S5, solidification and packaging step: after the colloid solidifies, take out the plastic bag and the electrophoresis gel sheet, and package them together for shipment.

4. The method for preparing electrophoresis gel according to claim 3, characterized in that: In S2, in the glue adding step, glue is injected using an AB mixing tube, wherein the glue solution in tube A contains an accelerator, tetramethylethylenediamine, and the solution in tube B contains a catalyst, ammonium persulfate.

5. The method for preparing electrophoresis gel according to claim 4, characterized in that: When preparing gradient glue, in S2, in the glue adding step, multiple glue adding positions are arranged on the production line, each glue adding position is used to inject glue solutions of different concentrations, and the container moves from the high concentration glue adding position to the low concentration glue adding position to add glue.

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