A portable carbon isotope labeling device for plant leaves

Abstract

The invention discloses a portable carbon isotope labeling device for plant leaves, which comprises: a bottle body and an upper cover; the bottle body is provided with a one-way air-permeable valve, a first air-filling tube and a second air-filling tube, and the one-way air-permeable valve, the first air-filling tube and the second air-filling tube are respectively arranged on both sides of the bottle body; the upper cover comprises: a main cover, a sub-cover and a sealing assembly; the sub-cover is sealed and connected with the main cover through the sealing assembly; a leaf stem through hole is arranged at the sealing connection between the main cover and the sub-cover; after the marked plant branches and leaves and stems are sealed and wrapped with a sealing band, they can be closely fitted in the leaf stem through hole; the gas in the bottle containing the plant leaves is flushed with nitrogen, and after the flushing is completed, the gas is flushed with nitrogen at a room temperature for labeling. 13 CO2 is filled to complete the carbon isotope labeling; this method reduces the impact of factors such as the gas and temperature in the labeling bottle on the carbon isotope labeling of plant leaves during labeling, and improves the efficiency and reliability of stable carbon isotope labeling of plant leaves.

Description

Technical Field

[0001] The invention relates to the technical field of stable isotope labeling, and in particular to a portable carbon isotope labeling device for plant leaves. Background Art

[0002] Carbon in plants is the main element of plant metabolism and participates in the process of plant photosynthesis. Carbon isotope labeling can be used as an important way to evaluate plant physiological mechanisms, and carbon isotope metabolism can be used as an important indicator for analyzing plant physiological mechanisms.

[0003] When plants are labeled with stable carbon isotopes, usually on sunny days with sufficient light, they perform photosynthesis while converting carbon dioxide and water into energy-rich organic matter and releasing oxygen. 13 CO2), thus completing the labeling of stable isotopes.

[0004] In the past, plant leaves were labeled by covering other tissues, which could not guarantee the absorption of stable carbon isotopes during plant respiration. Therefore, designing a convenient and accurate method for labeling plant leaves with stable carbon isotopes has become a technical problem that needs to be solved urgently. Summary of the invention

[0005] The purpose of the present invention is to solve the above problems and provide a portable carbon isotope labeling device for plant leaves and a carbon isotope labeling method for plant leaves;

[0006] A portable carbon isotope labeling device for plant leaves, comprising: a bottle body 1, an upper cover 2;

[0007] The bottle body 1 is provided with a one-way air-permeable valve 12, a first air-inflating tube 13, and a second air-inflating tube 14. The one-way air-permeable valve 12 and the first air-inflating tube 13 and the second air-inflating tube 14 are respectively arranged on both sides of the bottle body 1;

[0008] The upper cover 2 comprises: a main cover 21, a sub-cover 22, and a sealing assembly; the sub-cover 22 is sealed and connected to the main cover 21 via the sealing assembly; and a leaf stem through hole is provided at the sealed connection between the main cover 21 and the sub-cover 22.

[0009] The main cover 21 is provided with a notch 211; a slot notch I is provided on one side of the notch 211;

[0010] The auxiliary cover 22 is provided with a sealing portion, a slot notch II 222, and a shaft connection hole 223; the sealing portion is provided at one end of the auxiliary cover 22; the slot notch II 222 is provided at a side vertical surface of the sealing portion;

[0011] The leaf stem through hole is mainly formed by the butt joint of the slot notch I and the slot notch II 222 .

[0012] The sealing assembly includes: a connecting rod 231, a sealing rubber pad 232, a spring 233, a gasket 234, a retaining spring 235, and a knob 236;

[0013] The sealing rubber gasket 232 is sleeved on the lower end of the connecting rod 231 and is axially connected to the auxiliary cover 22 and the main cover 21 in sequence; the gasket 234 is fixed to the upper part of the connecting rod 231 by the retaining ring 235; the spring 233 is sleeved in the connecting rod 231, so that the lower contact surface of the spring 233 abuts against the upper end of the center of the main cover 21, and the upper contact surface of the spring 233 abuts against the lower contact surface of the gasket 234; the knob 236 is tightly connected to the top of the connecting rod 231.

[0014] The invention discloses a portable carbon isotope labeling device for plant leaves, which comprises: a bottle body 1 and an upper cover 2; the bottle body 1 is provided with a one-way air-permeable valve 12, a first air-filling tube 13 and a second air-filling tube 14, and the one-way air-permeable valve 12, the first air-filling tube 13 and the second air-filling tube 14 are respectively arranged on both sides of the bottle body 1; the upper cover 2 comprises: a main cover 21, a sub-cover 22 and a sealing component; the sub-cover 22 is sealed and connected with the main cover 21 through the sealing component; a leaf stem through hole is arranged at the sealed connection between the main cover 21 and the sub-cover 22; after the marked plant branches and leaves and stems are sealed and wrapped with a sealing band, they can be closely fitted in the leaf stem through hole; the gas in the bottle containing the plant leaves is flushed with nitrogen, and after the flushing is completed, the gas is flushed with nitrogen at a room temperature for labeling. 13 CO2 is filled to complete the carbon isotope labeling; this method reduces the impact of factors such as the gas and temperature in the labeling bottle on the carbon isotope labeling of plant leaves during labeling, and improves the efficiency and reliability of stable carbon isotope labeling of plant leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is an overall schematic diagram of a portable carbon isotope labeling device for plant leaves of the present invention;

[0016] Figure 2 It is a schematic cross-sectional view of the bottle body of a portable carbon isotope labeling device for plant leaves of the present invention;

[0017] Figure 3 It is a schematic diagram of a connecting rod of a portable carbon isotope labeling device for plant leaves of the present invention;

[0018] Figure 4 It is a schematic diagram of an exploded upward view of the upper cover of a portable carbon isotope labeling device for plant leaves according to the present invention;

[0019] Figure 5 It is a schematic diagram of opening the upper cover of a portable carbon isotope labeling device for plant leaves of the present invention;

[0020] Figure 6 The present invention is a schematic diagram of a portable carbon isotope labeling device for plant leaves in which an upper cover clamps leaves. DETAILED DESCRIPTION

[0021] Example 1 A portable carbon isotope labeling device for plant leaves

[0022] See also Figures 1 to 4 As shown, a portable carbon isotope labeling device for plant leaves comprises: a bottle body 1, an upper cover 2;

[0023] The upper end of the bottle body 1 is provided with a bottle mouth 11, and the bottle mouth 11 is provided with an internal threaded opening 111;

[0024] The bottle body 1 is provided with a one-way air-permeable valve 12, a first air-inflating tube 13, and a second air-inflating tube 14. The one-way air-permeable valve 12 and the first air-inflating tube 13 and the second air-inflating tube 14 are respectively arranged on both sides of the bottle body 1;

[0025] The first inflation pipe 13 is provided with a first inflation control valve 131, and one end of the first inflation pipe 13 is provided with a first inflation nozzle 132;

[0026] The second inflation pipe 14 is provided with a second inflation control valve 141; one end of the second inflation pipe 14 is provided with a second inflation nozzle 142;

[0027] The bottle body 1 is also provided with a hanging ring 15, and the bottle body 1 is hung on the branches of the marked plant through the hanging ring 15;

[0028] The upper cover 2 comprises: a main cover 21, a secondary cover 22, and a sealing assembly;

[0029] The main cover 21 is circular with a notch 211, which occupies about a quarter of the whole. A slot notch I is provided on one side of the notch 211. The vertical surface of the notch 211 is an inner bevel.

[0030] The lower edge of the outer ring of the main cover 21 is provided with a screw thread Ⅰ2141; the middle of the main cover 21 is provided with an axial hole;

[0031] The auxiliary cover 22 is provided with a sealing portion, a slot notch II 222, and a shaft connection hole 223;

[0032] The sealing part is arranged at one end of the auxiliary cover 22, and the vertical surface of the sealing part is an outer inclined surface; the card slot notch II 222 is arranged at the vertical surface of one side of the sealing part;

[0033] The vertical surface of the sealing portion is an outer bevel; the outer bevel of the sealing portion and the inner bevel of the notch portion 211 increase the sealing performance, so that the sealing portion can be completely sealed with the notch portion 211 of the main cover 21;

[0034] The slot notch I and the slot notch II 222 form a leaf stem through hole;

[0035] The outer ring vertical surface of the auxiliary cover 22 is provided with a screw thread II 224; the main cover 21 and the auxiliary cover 22 are integrated into one body, and the screw thread I 2141 and the screw thread II 224 form the sealing screw buckle of the upper cover 2;

[0036] The sealing assembly includes: a connecting rod 231, a sealing rubber pad 232, a spring 233, a gasket 234, a retaining spring 235, and a knob 236;

[0037] The connecting rod 231 is a multi-step rotating body; the lower end of the connecting rod 231 is a lower brim 2311, the upper part of the lower brim 2311 is provided with a first throat rod 2312, the upper part of the first throat rod 2312 is provided with a lower rod shaft 2313, and the diameter of the first throat rod 2312 is ≤ the diameter of the lower rod shaft 2313;

[0038] The lower rod shaft 2313 is provided with a main rod, the upper end of which is provided with a second throat rod 2314, the upper end of which is provided with a bayonet portion 2315; the upper end of the bayonet portion 2315 is provided with a knob connection portion 2316;

[0039] The sealing rubber pad 232 is provided with a sleeve hole 2321 and a stem and vine opening 2322;

[0040] The sleeve hole 2321 is sleeved on the first throat rod 2312; the lower rod shaft 2313 is axially connected to the shaft connection hole 223 of the auxiliary cover 22; the main rod shaft of the connecting rod 231 is connected to the shaft hole of the main cover 21; the gasket 234 is sleeved on the second throat rod 2314; the retaining ring 235 is locked in the bayonet portion 2315; the spring 233 is sleeved in the connecting rod 231, and the lower contact surface of the spring 233 is against the upper end of the center of the main cover 21, and the upper contact surface of the spring 233 is against the lower contact surface of the gasket 234; the knob 236 is tightly connected to the knob connecting portion 2316.

[0041] Example 2 A portable carbon isotope labeling method for plant leaves

[0042] The labeling method described in this embodiment uses a portable carbon isotope labeling device for plant leaves described in Example 1;

[0043] During the marking process, the marking device needs to be placed in a place with good sunlight, or on the side of the plant with good light; the marking time is generally selected between 8:30 am and 15:30 pm, so that the leaves of the plants to be marked can fully absorb light and carry out photosynthesis;

[0044] A portable carbon isotope labeling method for plant leaves, the steps are as follows:

[0045] 1) Remove the upper cover 2 from the bottle body;

[0046] 2) See attached Figure 5As shown, the knob 236 on the main cover 21 is pressed and twisted to separate the auxiliary cover 22 from the notch 211 on the main cover 21;

[0047] 3) Use sealing bands (bands used for plant grafting) to seal and wrap the marked plant branches, leaves, and stems, so that the bands, the slot notch I, and the slot notch II 222 form a leaf stem through hole that fits tightly;

[0048] 4) See Appendix Figure 6 , the marked plant leaves face downward, select a marked leaf or two leaves with the same leaf stem; insert the leaf stem into the leaf stem through hole, press the knob 236 on the main cover 21, and twist the auxiliary cover 22 to return to the notch 211 on the main cover 21; the main cover 21 and the auxiliary cover 22 are inclined surfaces that fit each other tightly, and the upward force of the spring 233 increases the tightness between the main cover 21 and the auxiliary cover 22;

[0049] 5) Place the leaves in the bottle and tighten the bottle cap 2;

[0050] 6) After placing the marked plant leaves in the bottle, first starve the plant leaves to reduce the gas content in the bottle;

[0051] 7) Connect the normal temperature nitrogen bag through the first inflation nozzle 132 of the first inflation tube 13; connect the normal temperature nitrogen bag for marking through the first inflation nozzle 142 of the second inflation tube 14. 13 CO2 stable isotope gas bag;

[0052] 8) Open the first inflation control valve 131 of the first inflation pipe 13, and fill the bottle with nitrogen at room temperature at a uniform speed by manually squeezing or using an air pump, and let the nitrogen pass through the one-way air permeable valve 12 to discharge other gases in the bottle; the inflation time is 2 minutes;

[0053] 9) The volume of stable carbon isotope gas injected during labeling is 5 ml, and the labeling time is controlled to be 30 minutes.

[0054] The technical implementation scheme of the present invention is described in detail above in conjunction with the accompanying drawings. The present invention provides a portable carbon isotope labeling device and a carbon isotope labeling method for plant leaves. After the labeled plant branches and stems are sealed and wrapped with a sealing band, they can fit tightly in the leaf stem through-holes of the upper cover; the gas in the bottle containing the plant leaves is flushed with nitrogen, and after the flushing is completed, the gas in the bottle containing the plant leaves is flushed with nitrogen at room temperature for labeling. 13 CO2 is filled to complete the carbon isotope labeling; this method reduces the impact of factors such as the gas and temperature in the labeling bottle on the carbon isotope labeling of plant leaves during labeling, and improves the efficiency and reliability of stable carbon isotope labeling of plant leaves.

Claims

1. A portable carbon isotope labeling device for plant leaves, comprising: A bottle body (1) and an upper cover (2); the characteristics are: the bottle body (1) is provided with a one-way air-permeable valve (12), a first air-inflating tube (13), and a second air-inflating tube (14); the one-way air-permeable valve (12) and the first air-inflating tube (13), and the second air-inflating tube (14) are respectively arranged on both sides of the bottle body (1); The upper cover (2) comprises: a main cover (21), a sub-cover (22), and a sealing assembly (23); the sub-cover (22) is sealedly connected to the main cover (21) via the sealing assembly (23); a leaf stem through hole is provided at the sealing connection between the main cover (21) and the sub-cover (22); The first inflation tube (13) is provided with a first inflation control valve (131), and one end of the first inflation tube (13) is provided with a first inflation nozzle (132); the second inflation tube (14) is provided with a second inflation control valve (141); one end of the second inflation tube (14) is provided with a second inflation nozzle (142); the bottle body (1) is also provided with a hanging ring (15); The main cover (21) is provided with a notch portion (211); a slot notch I is provided on one side of the notch portion (211); The auxiliary cover (22) is provided with a sealing portion, a slot notch II (222), and a shaft connection hole (223); the sealing portion is provided at one end of the auxiliary cover (22); the slot notch II (222) is provided at a side vertical surface of the sealing portion; The leaf stem through hole is formed by the butt joint of the slot notch I and the slot notch II (222).

2. The portable carbon isotope labeling device for plant leaves according to claim 1, characterized in that: The sealing assembly comprises: a connecting rod (231), a sealing rubber pad (232), a spring (233), a gasket (234), a retaining spring (235), and a knob (236); The sealing rubber gasket (232) is sleeved on the lower end of the connecting rod (231) and is axially connected to the auxiliary cover (22) and the main cover (21) in sequence; the gasket (234) is fixed to the upper part of the connecting rod (231) by a retaining ring (235); the spring (233) is sleeved in the connecting rod (231), so that the lower contact surface of the spring (233) abuts against the upper end of the center of the main cover (21), and the upper contact surface of the spring (233) abuts against the lower contact surface of the gasket (234); the knob (236) is tightly connected to the top of the connecting rod (231).

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