Traditional Chinese medicine preparation for preventing and treating fish myxosporidia and preparation method thereof

Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a traditional Chinese medicine preparation for preventing and treating fish myxosporidia and a preparation method thereof. The traditional Chinese medicine preparation is prepared by taking herba artemisiae annuae, artemisia scoparia, ovateleaf holly bark, garden balsam stem, periploca sepium bunge, folium eucalypti and tripterygium wilfordii as raw materials, matching the materials strictly according to monarch, minister, assistant and guide, and performing the processes of boiling extraction, concentration, alcohol precipitation and the like; traditional Chinese medicine extracts are good in safety; and the traditional Chinese medicine preparation has strong insecticidal and insect-inhibiting effects, can be used for feeding after being mixed with the materials, has the characteristics of high utilization rate and rapid medicine effect release, improves the weight growth rate and the feed return rate of fishes, and can effectively improve the autoimmunity and production performance of the fishes.

Description

technical field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a traditional Chinese medicine preparation for preventing and treating fish myxospores and a preparation method thereof. Background technique

[0002] The pathogen of fish myxosporiosis is pathogenic myxosporia, which belongs to Myxosporia, Myxosporia, and all of them live as parasites. There are many types of myxospores, the common ones are iodine worms, monopolar worms, bipolar worms, tetrapolar worms, myxosomal worms and cercospora. Myxospores can be seen all year round and are suitable for breeding in water temperatures between 8°C and 25°C. However, during the low temperature seasons of winter and spring, they can easily attack various organs and tissues of fish, seriously endangering the health of fish. It mainly harms crucian carp and carp, and fish can be infected with myxospore no matter whether it is in the fry stage or the adult stage. Incidence water...

Examples

Embodiment 1

[0023] Embodiment 1: The traditional Chinese medicine preparation for the prevention and treatment of fish myxospores of the present embodiment includes 500 g of raw materials Artemisia annua, 300 g of Artemisia scoparia, 500 g of jellybean, 500 g of Speranthema spp., 800 g of willow, and 1000 g of eucalyptus leaves in the following weights , Tripterygium 500g.

[0024] Preparation

[0025] The above raw materials and water were boiled and extracted for 2 hours according to the ratio of 1:10, extracted twice, the filtrates were combined twice, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:2; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

Embodiment 2

[0026] Embodiment 2: The traditional Chinese medicine preparation for the prevention and treatment of fish myxospores of the present embodiment includes the raw materials of the following weights: 300g Artemisia annua, 400g Artemisia scoparia, 700g parts of jellybean, 400g Speranthema, 600g, and 1200g Eucalyptus leaves , Tripterygium wilfordii 200g.

[0027] Preparation

[0028] The above raw materials and water were boiled and extracted for 3 hours according to the ratio of 1:8, extracted 3 times, and the filtrates were combined for 3 times, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:1; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

Embodiment 3

[0029] Embodiment 3: the traditional Chinese medicine preparation of preventing and treating fish myxosporia of the present embodiment, comprises the raw material Artemisia annua 300g, Artemisia scoparia 400g, 700g part of jellybean, 400g of Speranthema cerevisiae, 600g of willow bark, 1200g of eucalyptus leaf , Tripterygium wilfordii 200g.

[0030] The above raw materials and water were boiled and extracted for 2 hours according to the ratio of 1:12, extracted 3 times, and the filtrates were combined for 3 times, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:3; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

technical field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a traditional Chinese medicine preparation for preventing and treating fish myxospores and a preparation method thereof. Background technique

[0002] The pathogen of fish myxosporiosis is pathogenic myxosporia, which belongs to Myxosporia, Myxosporia, and all of them live as parasites. There are many types of myxospores, the common ones are iodine worms, monopolar worms, bipolar worms, tetrapolar worms, myxosomal worms and cercospora. Myxospores can be seen all year round and are suitable for breeding in water temperatures between 8°C and 25°C. However, during the low temperature seasons of winter and spring, they can easily attack various organs and tissues of fish, seriously endangering the health of fish. It mainly harms crucian carp and carp, and fish can be infected with myxospore no matter whether it is in the fry stage or the adult stage. Incidence water...

Examples

Embodiment 1

[0023] Embodiment 1: The traditional Chinese medicine preparation for the prevention and treatment of fish myxospores of the present embodiment includes 500 g of raw materials Artemisia annua, 300 g of Artemisia scoparia, 500 g of jellybean, 500 g of Speranthema spp., 800 g of willow, and 1000 g of eucalyptus leaves in the following weights , Tripterygium 500g.

[0024] Preparation

[0025] The above raw materials and water were boiled and extracted for 2 hours according to the ratio of 1:10, extracted twice, the filtrates were combined twice, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:2; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

Embodiment 2

[0026] Embodiment 2: The traditional Chinese medicine preparation for the prevention and treatment of fish myxospores of the present embodiment includes the raw materials of the following weights: 300g Artemisia annua, 400g Artemisia scoparia, 700g parts of jellybean, 400g Speranthema, 600g, and 1200g Eucalyptus leaves , Tripterygium wilfordii 200g.

[0027] Preparation

[0028] The above raw materials and water were boiled and extracted for 3 hours according to the ratio of 1:8, extracted 3 times, and the filtrates were combined for 3 times, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:1; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

Embodiment 3

[0029] Embodiment 3: the traditional Chinese medicine preparation of preventing and treating fish myxosporia of the present embodiment, comprises the raw material Artemisia annua 300g, Artemisia scoparia 400g, 700g part of jellybean, 400g of Speranthema cerevisiae, 600g of willow bark, 1200g of eucalyptus leaf , Tripterygium wilfordii 200g.

[0030] The above raw materials and water were boiled and extracted for 2 hours according to the ratio of 1:12, extracted 3 times, and the filtrates were combined for 3 times, filtered and concentrated to obtain the concentrated solution; the concentrated solution and 75% ethanol were alcohol-precipitated for 24 hours according to the ratio of 1:3; The supernatant after alcohol precipitation was filtered with slow filter paper; the filtrate was concentrated to 1 g (raw medicinal material) / mL with a rotary evaporator.

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