A kind of aquaculture wastewater bacteria and algae symbiotic small ball bioreactor

Abstract

A biological reaction device for bacteria-algae symbiotic pellets for aquaculture wastewater, comprising at least one reaction box placed in a biological pool, characterized in that the reaction box comprises a bracket and a filter screen mounted on the bracket and formed with the bracket to be divided from top to bottom into a first reaction chamber and a second reaction chamber; and both sides of each of the reaction boxes are connected to an installation box with a first aeration pipe and a light compensation member disposed therein. The reaction device provided by the present invention can be directly placed in a biological filter pool for culture reaction, and the device can realize the cycle culture of bacteria-algae symbiosis, and can control the growth conditions of bacteria-algae pellets at different depths to be consistent, with high wastewater treatment efficiency and good effect.

Description

Technical Field

[0001] The invention belongs to the technical field of bacteria-algae ball reaction devices, and in particular relates to a bacteria-algae symbiotic ball biological reaction device for aquaculture wastewater. Background Art

[0002] In recent years, my country's aquaculture industry has developed rapidly, with an annual aquaculture output of nearly 50 million tons, making great contributions to promoting national economic development and meeting people's demand for aquatic products. However, driven by economic interests for a long time, people often adopt high-input, high-emission methods to achieve high output. Waste such as leftover bait, excrement, and dead organisms in the aquaculture process has increased significantly, causing pollutants such as nitrogen and phosphorus in the water to exceed the natural purification capacity of the water body. A large amount of tail water discharged from the farm has caused eutrophication pollution in surrounding rivers and lakes.

[0003] The immobilized bacteria-algae symbiotic microspheres utilize the synergistic effect of bacteria and microalgae to achieve the purpose of degrading organic matter in water bodies and removing nitrogen and phosphorus. They have the advantages of high biological density and high efficiency of denitrification and phosphorus removal in wastewater treatment. Aerobic bacteria degrade organic matter into soluble inorganic substances that can be used by microalgae. The energy generated during the decomposition process provides energy for the metabolism of bacteria themselves; microalgae absorb and convert the substances decomposed by bacterial metabolism into their own cell substances, and release oxygen through photosynthesis, increasing the concentration of dissolved oxygen in the water body. Sufficient dissolved oxygen concentration can satisfy aerobic bacteria to maintain their normal life metabolic activities. Since bacteria and algae grow in a suspended state and are easily lost from the reactor, the sewage treatment time is increased and the solid-liquid separation in the later stage is difficult. The immobilized bacteria-algae symbiotic microspheres adsorb or embed bacteria and algae through materials to form a stable biofilm or spatial structure, realizing the separation of bacteria and algae retention and hydraulic retention in time and space, making wastewater denitrification and phosphorus removal more efficient, and helping to improve the impact resistance and denitrification and phosphorus removal efficiency of the wastewater pollution load.

[0004] The existing method of fixing the bacteria-algae symbiotic microspheres in the biofilter is generally as follows: an upper support plate and a lower support plate are provided in the biofilter, an aeration head is provided at the bottom of the filter and below the lower support plate, lighting tubes are installed around the pool wall between the support plate and the lower support plate, and the bacteria-algae microspheres are distributed between the upper support plate and the lower support plate, and the distribution density, temperature, and light intensity are controlled so that the bacteria-algae microspheres grow in the biofilter to treat wastewater. However, this technology has the problem that the growth conditions of the bacteria-algae microspheres at the bottom and the top of the filter are different, and the bacteria-algae microspheres at the bottom grow slowly. The document with application number CN201920424395.6 discloses a kind of photobiological reaction device of bacteria and algae ball, including a tank body, a stirring device, a light compensation device and a filter screen, the tank body is a vertical column structure, the tank body is a transparent structure, a water outlet is arranged at the bottom of the tank body, a water inlet is arranged at the top of the tank body, the filter screen is arranged above the water outlet, the filter screen is arranged along the horizontal cross section of the cylinder body and completely covers the cross section, the tank body is provided with a separation port on the circumferential surface, the stirring device includes a motor, a stirring shaft and a stirring blade, the stirring shaft is coaxially arranged inside the tank body, the stirring shaft and the tank body, the motor is arranged at the top of the tank body, the motor output shaft is coaxially fixedly connected with the stirring shaft, the stirring blade is arranged along the circumferential surface of the stirring shaft, and the light compensation device is arranged on the circumferential surface of the stirring shaft. The beneficial effect of the utility model is that the cyclic cultivation of bacteria and algae symbiosis can be realized, and the growth efficiency can be improved. However, the stirring shaft and stirring blades provided by the device are easy to destroy the growth state of the bacteria and algae microspheres, and the device is only suitable for laboratories, not for being placed in biological filter tanks in actual scenes. Summary of the invention

[0005] In view of the above-mentioned problems, the purpose of the present invention is to provide a bioreactor for aquaculture wastewater with bacteria and algae symbiotic beads, which can be directly placed in a biological filter tank for cultivation reaction. The device can realize the cyclic cultivation of bacteria and algae symbiosis and can control the growth conditions of bacteria and algae balls at different depths to remain consistent, thereby achieving high wastewater treatment efficiency and good effect.

[0006] In order to achieve the above object, the technical solution of the present invention is as follows:

[0007] A bacteria-algae symbiotic pellet bioreactor for aquaculture wastewater, comprising at least one reaction box placed in a biological pool, characterized in that the reaction box comprises a bracket and a filter screen installed on the bracket and formed with the bracket to be divided from top to bottom into a first reaction chamber and a second reaction chamber; and both sides of each of the reaction boxes are connected to an installation box with a first aeration pipe and a light compensation member arranged inside.

[0008] In the present design, the advantages of dividing the reaction box into two reaction chambers through which the bacteria and algae balls cannot pass are: first, it avoids the problem that after the reaction box is placed in the biological pool, the bacteria and algae balls in the deep area move toward the shallow area towards the light, causing excessive density to affect growth; second, after a period of reaction, the reaction box as a whole can be turned upside down so that the two reaction chambers can be interchanged, so as to achieve the same growth conditions of the bacteria and algae balls in the two reaction chambers and avoid the problem of inconsistent cultivation in deep and shallow areas; finally, aeration pipes and light compensation parts are still installed in the deep area, which is beneficial to the growth of bacteria and algae balls in the deep area, and the aeration pipes and light compensation parts are installed in the installation box to reduce wear and tear, which can also be beneficial to the positioning of the reaction box.

[0009] As a further preferred embodiment of the present invention, the upper and lower ends of the reaction box body are respectively provided with installation frames connected to the vertical plates of the bracket for installing the filter screen, and the two side surfaces of the reaction box body connected to the installation box are respectively provided with air permeable nets installed on the vertical plates.

[0010] As a further preferred embodiment of the present invention, support plates are respectively provided at the two side surfaces where the reaction box is connected to the installation box, the two ends of which are connected to the vertical plates, located between the first reaction chamber and the second reaction chamber, and respectively provided with a first opening connected to the first reaction chamber and a second opening connected to the second reaction chamber. This design reduces the solid area, facilitates the air permeability inside the reaction box, and is beneficial to the growth of bacteria and algae balls.

[0011] As a further preferred embodiment of the present invention, the installation frame is provided with a first fixing frame adapted to a first installation groove opened on the installation frame for placing the first filter screen.

[0012] As a further preferred embodiment of the present invention, the breathable net is connected to the vertical plate by being fixed on a second fixing frame that is engaged with a second mounting groove provided on the vertical plate. This design not only ensures the tightness of the installation, but also facilitates the removal and replacement of the breathable net.

[0013] As a further preferred embodiment of the present invention, a third mounting groove for mounting a third fixing frame provided with a third filter screen is provided on the vertical plate, and a second fixing column for fixing the third fixing frame and a second fixing bar with one end fixed to the vertical plate by the second fixing column and the other end rotating around the second fixing column as the center are also provided on the vertical plate.

[0014] As a further preferred embodiment of the present invention, the installation frame is provided with a first fixing column for fixing the first fixing frame and a first fixing bar with one end fixed to the installation frame by the first fixing column and the other end rotating around the first fixing column as the center of a circle, and a friction block is provided at the contact point between the first fixing bar and the first fixing frame.

[0015] As a further preferred embodiment of the present invention, a protrusion with a minor arc or semicircular cross-section is provided on the lower surface of the first fixing frame.

[0016] As a further preferred embodiment of the present invention, a sealing layer is provided on the outer side of the second fixing frame.

[0017] As a further preferred embodiment of the present invention, an aeration main pipe connected to the first aeration pipe and passing through a channel opened on a support base connected to the lower bottom surface of the reaction box is provided between the bottom surface of the biological pond and the lower bottom surface of the reaction box.

[0018] Furthermore, the preparation process of the bacterial algae ball of the present invention is as follows: (1) Under aseptic operation conditions, inoculate the four-tailed scenedesmus or protein nucleus chlorella in SE culture medium at an inoculation amount of 1×106 cells / mL×107 cells / mL, and culture the cells at a temperature of 25°C-27°C, a pH value of 6.5-7, a rotation speed of 110-130r / min, and alternately culture the cells under light and dark cycles, with a light intensity of 3000-4000Lx. After culturing for 60-75h, obtain the microalgae culture solution, centrifuge at 5000-6000r / min for 5-10min, wash with distilled water, centrifuge to remove inorganic salts and other substances attached to the algae cells, and store at 4°C for standby use. (2) Take a certain volume of activated sludge from a municipal sewage treatment plant, dilute the activated sludge with sterile water, apply it to a bromothymol blue (BTB) plate culture medium, and culture it at 30°C for 2d. Select the blue-surrounded colonies on the plate, separate and purify them by streaking on beef extract peptone plate medium, inoculate them into heterotrophic nitrification culture medium, and culture them at 30℃ for 2 days. The bacteria with a significantly reduced ammonia nitrogen concentration in the culture medium are heterotrophic nitrification-aerobic denitrification bacteria. Inoculate 10% (volume ratio) of the bacterial solution in the logarithmic growth phase into a conical flask of DM denitrification culture medium, culture it at 30℃ and 160 r / min overnight, concentrate it by centrifugation at 8000 r / min, and store it at 4℃ for later use. (3) Mix the algae solution and bacterial solution in a ratio of 1:1 to prepare a composite bacterial-algae suspension. (4) Weigh a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) and mix them with water. The concentration of polyvinyl alcohol (PVA) is 2% and the concentration of sodium alginate is 3%. Heat it to 100℃ in a water bath until it is completely dissolved and then cool it to room temperature. (5) Add the prepared composite bacterial-algae suspension to the cooled polyvinyl alcohol (PVA) and sodium alginate (SA) mixture at a ratio of 30:6 (volume ratio) and mix well. (6) Suck the above mixture into a syringe and slowly drip into a 3% calcium chloride solution. Soak the formed pellets in the calcium chloride solution and place them at 20°C for 4 hours. Then take them out, wash them with distilled water 3 times, and place them in a culture dish to dry to obtain the immobilized pellets.

[0019] In summary, the present invention has the following beneficial effects:

[0020] The reaction device of the present invention can be directly placed in a biological pool and has a wide application range.

[0021] The reaction device of the present invention can achieve the same growth conditions of bacteria and algae balls in two reaction chambers, solving the problem of inconsistent cultivation in deep and shallow areas.

[0022] The movement and fixation of the reaction device of the present invention itself do not require the aid of any electric control equipment or adjusting parts such as air pumps, and are easy to disassemble and maintain, so they can be mass-produced, thereby reducing production costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Attached Figure 1 It is a schematic structural diagram of the reaction device of the present invention.

[0024] Attached Figure 2 It is a schematic structural diagram of the left and right sides of the reaction device of the present invention.

[0025] Attached Figure 3 It is a schematic structural diagram of the upper and lower sides of the reaction device of the present invention.

[0026] Attached Figure 4 It is a schematic structural diagram of the front and rear sides of the reaction device of the present invention.

[0027] Attached Figure 5 It is a partial structural schematic diagram of the front and rear sides of the reaction device of the present invention.

[0028] Attached Figure 6 It is a partial structural schematic diagram of the reaction box of the reaction device of the present invention.

[0029] Description of the drawings: biological pool 1, reaction box 2, first reaction chamber 201, second reaction chamber 202, bracket 21, mounting frame 211, first mounting groove 211a, vertical plate 212, second mounting groove 212a, third mounting groove 212b, third fixing groove 212b1, second fixing column 212c1, second fixing bar 212c2, support plate 213, first opening 213a, second opening 213b, first fixing frame 214a, protrusion 214a1, first fixing column 2 14b, a first fixing bar 214c, a friction block 214c1, a second fixing frame 215a, a second mounting block 215a1, a sealing layer 215a2, a breathable net 215b, a third fixing frame 216a, a third mounting block 216a1, a filter 22, a first filter 221, a third filter 223, a supporting base 24, a channel 241, an installation box 3, a first aeration pipe 31, a first aeration port 311, an optical compensation member 32, a fixing bolt 321, an aeration main pipe 4, and an aeration hole 41. DETAILED DESCRIPTION

[0030] Example 1

[0031] As attached Figure 1 As shown, the present embodiment provides a bioreactor for bacteria-algae symbiotic pellets in aquaculture wastewater, comprising three parallel reaction boxes 2 placed in a biological pool 1, and an installation box 3 is placed between two adjacent reaction boxes 2, the reaction box 2 is mainly composed of a bracket 21 and a filter screen 22 installed on the bracket 21, and the filter screen 22 divides the reaction box 2 from top to bottom into two identical first reaction chambers 201 and second reaction chambers 202, the reaction chambers are used to place cultured bacteria-algae symbiotic pellets; the reaction box 2 is composed of two identical upper and lower installation frames 211, two identical front and rear vertical plates 212, and two left and right support plates 213 for connecting the vertical plates 212.

[0032] As attached Figure 3 As shown, in this embodiment, each mounting frame 211 is provided with a first mounting groove 211a for placing the first filter screen 221, and a first fixing frame 214a for fixing the first filter screen 221 is installed on the first filter screen 221. The first fixing frame 214a is also embedded in the first mounting groove 211a, and the upper surface of the first fixing frame 214a is flush with the upper surface of the mounting frame 211; further, in order to further tighten the first fixing frame 214a, a fixing column 214b is installed on the upper surface of the mounting frame 211 to fix the first filter screen 221. The rotatable first fixing bar 214c, when the first fixing frame 214a is installed, the first fixing bar 214c is rotated onto the first fixing frame 214a and abutted against the upper surface of the first fixing frame 214a, and in order to prevent the first fixing bar 214c from sliding, a friction block 214c1 for increasing friction is installed on the lower surface of the end of the first fixing bar 214c abutting against the first fixing frame 214a, and the material of the friction block 214c1 can be selected from metal / rubber with particles or stripes or other materials that can increase friction.

[0033] As attached Figure 2As shown, in this embodiment, the left and right sides of the reaction box body 2 are connected to the installation box 3, and each single side is composed of a solid support plate 213 and an air-permeable layer composed of an air-permeable net 215b distributed on the upper and lower sides of the support plate 213. The support plate 213 is provided with a first opening 213a for connecting to the first reaction chamber 201, and a second opening 213b for connecting to the second reaction chamber 202. The opening is provided for conveying / discharging bacteria and algae pellets and has a cover on the opening; in order to fix the air-permeable net 215b, the air-permeable net 215b is first fixedly installed on the second fixing frame 215a, and two second mounting blocks 215a1 for fixing are formed at both side ends of the second fixing frame 215a, and two second mounting grooves 212a are correspondingly opened on the vertical plates 212 at the two side ends for the second mounting blocks 215a1 to be embedded and fixed, and in order to prevent the existence of gaps, a sealing layer 215a2 for sealing is fixed around the second fixing frame 215a, and the sealing layer can be made of conventional rubber.

[0034] As attached Figure 4 , 5 As shown, in this embodiment, a third installation groove 212b for installing a third filter screen 223 that divides the internal space of the box body is opened in the middle of the front and rear vertical plates 212, and third fixing grooves 212b1 for limiting and fixing are formed at both sides of the third installation groove 212b. The third filter screen 233 is also first fixed on a third fixing frame 216a with third mounting blocks 216a1 formed at both ends, and then embedded in the third installation groove 212b; and in order to prevent the third fixing frame 216a from sliding forward and backward, the third fixing frame 216a is provided with a third fixing block 216a1 at both ends. The groove 212b is provided with a rotatable second fixing bar 212c2 installed on the vertical plate 212 and fixed by a second fixing column 212c1. When the third fixing frame 216a is installed, the second fixing bar 212c2 is rotated to the third fixing frame 216a and abutted against the upper surface of the third fixing frame 216a. In order to prevent the second fixing bar 212c2 from sliding, a friction block for increasing friction is installed on the lower surface of the end of the second fixing bar 212c2 abutting against the third fixing frame 216a.

[0035] As attached Figure 6 As shown, in this embodiment, a side and a top surface of the installation box 3 connected to the reaction box body 2 are not provided with a solid plate, and a light compensation member 32 fixed by fixing bolts 321 is installed on the bottom surface of the installation box 3, and a first aeration pipe 31 with a plurality of first aeration ports 311 passes through the bottom surface of the box body and is connected to the aeration main pipe 4 laid on the bottom surface of the biological pool 1.

[0036] In this embodiment, the apertures of the first filter 221 and the third filter 223 are about 2 mm, and the light compensation component 32 is an existing waterproof lamp with a light intensity of 3000lux-4800lux. The waterproof structure includes a lamp body and a photosensitive chip. The photosensitive chip controls the current of the lamp body. The lamp body uses a light strip composed of LED lamp beads. The light compensation component sensed by the light source can automatically adjust the light intensity according to the natural light intensity of the outside world to make full use of natural light and save energy.

[0037] In this embodiment, the preparation method of the bacterial-algae symbiotic pellet is as follows: 1. Under aseptic operation conditions, inoculate the SE medium with Scenedesmus quadricauda or Chlorella pyrenoidosa at an inoculation amount of 1×106 / mL×107 / mL, and culture the medium at a temperature of 25°C-27°C, a pH value of 6.5-7, a rotation speed of 110-130r / min, and alternately culture the medium with light and dark cycles, with a light intensity of 3000-4000Lx. After culturing for 60-75h, obtain the microalgae culture solution, centrifuge at 5000-6000r / min for 5-10min, wash with distilled water, centrifuge to remove inorganic salts and other substances attached to the algae cells, and store at 4°C for standby use. 2. Take a certain volume of activated sludge from a municipal sewage treatment plant, dilute the activated sludge with sterile water, apply it to a bromothymol blue (BTB) plate medium, and culture it at 30°C for 2d. Select the blue colonies on the plate, separate and purify them by streaking on beef extract peptone plate medium, inoculate them into heterotrophic nitrification culture medium, shake and culture them at 30℃ for 2 days, and the strains with significantly reduced ammonia nitrogen concentration in the culture medium are heterotrophic nitrification-aerobic denitrification bacteria. Inoculate 10% (volume ratio) of the bacterial solution in the logarithmic growth phase into a conical flask of DM denitrification medium, culture it at 30℃ and 160 r / min overnight, centrifuge and concentrate it at 8000 r / min, and store it at 4℃ for later use. 3. Mix the centrifugally concentrated algae solution and bacterial solution in a 1:1 ratio to prepare a composite bacterial algae suspension.

[0038] 4. Weigh a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) and mix them with water. The concentration of polyvinyl alcohol (PVA) is 2% and the concentration of sodium alginate is 3%. Heat it to 100°C in a water bath until it is completely dissolved and then cool it to room temperature. 5. Add the prepared composite bacteria and algae suspension to the cooled polyvinyl alcohol (PVA) and sodium alginate (SA) mixture in a ratio of 30:6 (volume ratio) and mix them evenly. 6. Inhale the above mixture into a syringe and slowly drip into a 3% calcium chloride solution. Soak the formed pellets in the calcium chloride solution and place them at 20°C for 4 hours. Take them out, wash them 3 times with distilled water, and place them in a culture dish to dry to obtain immobilized pellets.

[0039] The principles of this embodiment are as follows:

[0040] After the reaction box 2 is assembled, the prepared bacteria-algae symbiotic balls with a diameter of 3-4 mm are divided into two equal parts and transported to the first reaction chamber 201 and the second reaction chamber 202 from the first opening 213a and the second opening 213b respectively, with the density controlled at 5500-6500 particles / dm 3 Within the range, after covering the cover, put the reaction box 2 into the biological pool 1 with the aeration main pipe 4 and the installation box 3 laid at the bottom, and then place the reaction box 2 as a whole into the biological pool 1, adjust the temperature to 25-30°C, and the light-dark ratio to 12h:12h. After the hydraulic retention time of the aquaculture wastewater in the biological pool 1 is 4h, remove the reaction box 2, turn it upside down and put it back into the biological pool 1, continue to cultivate the reaction, and then repeat the above steps. Finally, the wastewater treatment effect of the device is that the removal rates of ammonia nitrogen, nitrite and total phosphorus in the wastewater are all above 80%.

[0041] Example 2

[0042] In this embodiment, based on the first embodiment, a support base 24 is provided at the bottom of the biological pool 1 for positioning and fixing the reaction box 2, and a channel 241 is provided on the side of the support base 24 for fixing the aeration main pipe 4 with aeration holes 41; in order to further strengthen the fixing effect of the first fixing frame 214a on the first filter screen 221 to prevent the formation of gaps, a plurality of relatively low protrusions 214a1 with inferior arc or semicircular cross-sections are formed on the lower bottom surface of the first fixing frame 214a.

[0043] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A bacterial-algae symbiotic pellet bioreactor for aquaculture wastewater, comprising at least one reaction box (2) placed in a biological pool (1), characterized in that: The reaction box (2) is separated into a first reaction chamber (201) and a second reaction chamber (202) through which the bacteria-algae symbiotic balls cannot pass, and the first reaction chamber (201) and the second reaction chamber (202) are both provided with bacteria-algae symbiotic balls; the reaction box (2) comprises a support (21) and a filter screen (22) mounted on the support (21) and forming a partition with the support (21) to form the first reaction chamber (201) and the second reaction chamber (202) from top to bottom; and both sides of each reaction box (2) are connected to an installation box (3) in which a first aeration pipe (31) and a light compensation member (32) are provided; the first aeration pipe (31) has a plurality of aeration ports, and the filter screen (22) comprises a first filter screen (221) and a third filter screen (223), and the third filter screen (223) separates the internal space of the reaction box (2); The upper and lower ends of the reaction box body (2) are respectively provided with mounting frames (211) connected to the vertical plate (212) of the bracket (21) and used for mounting the first filter screen (221), and the two side surfaces of the reaction box body (2) connected to the mounting box (3) are respectively provided with air-permeable nets (215b) mounted on the vertical plate (212), and the mounting frame (211) is provided with a first fixing frame (214a) adapted to a first mounting groove (211a) provided on the mounting frame (211) and used for placing the first filter screen (221); Support plates (213) are provided at two sides of the reaction box body (2) connected to the installation box (3), the two ends of which are connected to the vertical plate (212), are located between the first reaction chamber (201) and the second reaction chamber (202), and are provided with a first opening (213a) communicating with the first reaction chamber (201) and a second opening (213b) communicating with the second reaction chamber (202); The vertical plate (212) is provided with a third mounting groove (212b) for mounting a third fixing frame (216a) provided with a third filter screen (223), and the vertical plate (212) is also provided with a second fixing column (212c1) for fixing the third fixing frame (216a) and a second fixing bar (212c2) having one end fixed to the vertical plate (212) by the second fixing column (212c1) and the other end rotating around the second fixing column (212c1) as a circle center; The installation frame (211) is provided with a first fixing column (214b) for fixing the first fixing frame (214a) and a first fixing bar (214c) having one end fixed to the installation frame (211) by the first fixing column (214b) and the other end rotating around the first fixing column (214b) as a circle center, and a friction block (214c1) is provided at the abutment between the first fixing bar (214c) and the first fixing frame (214a).

2. The aquaculture wastewater bacteria-algae symbiotic pellet bioreactor according to claim 1, characterized in that: The air-permeable net (215b) is connected to the vertical plate (212) by being fixed to a second fixing frame (215a) that is clamped to a second mounting groove (212a) provided on the vertical plate (212).

3. The aquaculture wastewater bacteria-algae symbiotic pellet bioreactor according to claim 1, characterized in that: The lower surface of the first fixing frame (214a) is provided with a protrusion (214a1) having a cross-section of a minor arc or a semicircle.

4. The aquaculture wastewater bacteria-algae symbiotic pellet bioreactor according to claim 2, characterized in that: A sealing layer (215a2) is provided on the outer side of the second fixing frame (215a).

5. The aquaculture wastewater bacteria-algae symbiotic pellet bioreactor according to claim 1, characterized in that: An aeration main pipe (4) is provided between the bottom surface of the biological pond (1) and the lower bottom surface of the reaction box (2), which is connected to the first aeration pipe (31) and passes through a channel (241) provided on a support base (24) connected to the lower bottom surface of the reaction box (2).

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