Flow sample pretreatment system and flow cytometer

Abstract

The present invention discloses a flow sample pretreatment system and a flow cytometer. The flow sample pretreatment system comprises a rack module, a sample arm module, a sample rack module, a sample puncture module, a reagent arm module, a reagent rack module, a reagent needle module, a centrifuge module, a clamping module, an oscillation vortex module and a control module. The sample puncture module is used for puncturing a sample container, the reagent needle module is used for adding a reagent in a reagent container into the sample container to form a second-generation sample container, the centrifuge module performs centrifugal treatment, the oscillation vortex module performs oscillation treatment, and the control module is used for providing control and / or operation for all components of the flow sample pretreatment system of the present invention. In this way, through automatic sample reagent transfer, automatic mixing, centrifugation, oscillation and other treatments are performed, a fully automated flow sample pretreatment process is realized, the test efficiency is greatly improved, the test error is reduced, and the safety is high.

Description

Technical Field

[0001] The present invention relates to a flow sample preprocessing system and a flow cytometer, and in particular to an efficient, safe, accurate and fully automated flow sample preprocessing system and a flow cytometer. Background Art

[0002] Flow cytometry and flow cytometry are a detection method for quantitative analysis and sorting of single cells or other biological particles. It can analyze thousands of cells at high speed in a short time and can measure multiple parameters from one cell at the same time. Flow cytometers are called CT in laboratories. Samples loaded on flow cytometers require complex sample pre-treatment, usually through a series of complex processing and separation processes such as manual, manual loading, shaking, centrifugation, etc. to prepare samples. Specifically, there are antibody screening in biopharmaceutical research and development, sample preparation for in vitro cell drug toxicity screening; sample preparation for gene protein detection of multidrug resistance; sample preparation for large-scale detection of cell multi-factors; sample preparation for large-scale detection of TBNK; sample preparation for other fully automatic detection scenarios in the replacement of manual operation of the laboratory and prevention of biological contamination, etc. The existing technology generally uses a pipette to manually add blood from a vacuum blood collection tube into the flow tube (the vacuum blood collection tube needs to be opened in advance), and then uses a pipette to add reagents and lysate into the flow tube, slowly shakes and centrifuges, and then uses a pipette to discard the supernatant and add PBS to prepare for the machine.

[0003] With the popularization of human immune index detection (various immunodeficiency diseases) and cellular multi-factors (early screening and typing of cancer), the number of flow cytometry samples is showing an increasing trend, and at the same time, the trend of full automation of clinical testing is gradually increasing. If we continue to use purely manual sample preparation, it is impossible to prepare large quantities of samples. As the operation time increases, the error rate increases, the consistency of processed samples cannot be fully guaranteed, and the sample processing rate is low. At the same time, the manual transfer of flow tubes to the centrifuge by personnel will pose a certain hidden danger to the safety of operators. In summary, it can be seen that the demand for the detection of flow cytometry samples is increasing, and there is an urgent need for a fully automatic flow sample pre-treatment equipment to replace manual flow sample preparation, so that the error in sample processing is zero, and all processing after sample collection is automated and intelligently controlled and managed. Summary of the invention

[0004] In response to the above technical problems, the present invention provides a flow sample pretreatment system and a flow cytometer, which realize a fully automated flow sample pretreatment process through automatic sample reagent transfer, automatic mixing, centrifugation, shaking and other processes, greatly improving test efficiency, reducing test errors, and having high safety.

[0005] In order to achieve one of the above-mentioned objects of the invention, the present invention provides a flow sample preprocessing system, the flow sample preprocessing system comprising:

[0006] Rack modules;

[0007] A sample arm module is movably mounted on the rack module;

[0008] A sample rack module, disposed below the sample arm module, for accommodating a plurality of sample containers and driving the plurality of sample containers to flip;

[0009] A sample puncture module is movably mounted on the sample arm module, and the sample puncture module comprises a sample needle, and the sample needle is used to puncture the sample container;

[0010] A reagent arm module is movably mounted on the rack module and is parallel to the sample arm module;

[0011] A reagent rack module, disposed below the reagent arm module, for accommodating at least one reagent container;

[0012] A reagent needle module is movably mounted on the reagent arm module, the reagent needle module comprises a reagent needle, and the reagent needle is used to add the reagent in the reagent container to the sample container to form a second-generation sample container;

[0013] A centrifuge module, disposed beside the sample rack module and / or the reagent rack module, for accommodating the second-generation sample container and performing centrifugal treatment on the second-generation sample container;

[0014] A clamping module, movably mounted on the reagent arm module, for grabbing and transferring the second-generation sample container;

[0015] An oscillation vortex module, disposed beside the centrifuge module, for accommodating the second-generation sample container and performing oscillation treatment on the second-generation sample container; and

[0016] The control module is used to control and / or operate all the elements of the flow sample preprocessing system to realize the automation of the flow sample preprocessing process.

[0017] As an optional technical solution, the flow sample pretreatment system further includes a needle washing pool module, and the needle washing pool module includes a sample needle washing pool and a reagent needle washing pool.

[0018] As an optional technical solution, the sample rack module includes:

[0019] A sample rack, used for holding a plurality of the sample containers;

[0020] a gear connected to one end of the sample holder; and

[0021] The first motor is arranged below the gear to drive the gear to drive the sample rack to flip.

[0022] As an optional technical solution, the flow sample preprocessing system further includes an identification module, which is disposed at the other end of the sample rack and is used to read the barcode on the sample container and / or the second-generation sample container.

[0023] As an optional technical solution, the sample puncture module further includes:

[0024] Grip part;

[0025] A second motor, used for driving the holding portion to move so as to hold the sample container; and

[0026] The third motor is used for driving the sample needle to puncture the sample container when the holding portion holds the sample container.

[0027] As an optional technical solution, the centrifuge module includes:

[0028] A centrifuge, comprising a cover body and an angle rotor, wherein a plurality of container insertion holes for inserting the second-generation sample containers are provided on the annular surface of the angle rotor;

[0029] A thickened bottom plate for carrying the centrifuge; and

[0030] The unbalance protection module is used to gradually reduce the speed of the centrifuge when it reaches a critical point of unbalance.

[0031] As an optional technical solution, the clamping module includes:

[0032] A plurality of grippers, surrounding and forming an automatically adjustable gripping space for gripping the second-generation sample container; and

[0033] The fourth motor is used to drive the plurality of clamps to move and rotate.

[0034] As an optional technical solution, the oscillating vortex module includes:

[0035] Oscillating device;

[0036] A clamping device, the clamping device comprising:

[0037] Chassis;

[0038] A collar, movably hinged to the chassis via a hinge;

[0039] an annular gasket fixed on the collar so that the second-generation sample container passes through the annular gasket and the collar in sequence; and

[0040] The oscillating vortex motor is used to drive the oscillating device to oscillate and vortex the second-generation sample container, and drive the ring to rotate to drive the annular gasket to contract, thereby clamping and loosening the second-generation sample container.

[0041] As an optional technical solution, the reagent needle module further includes a fifth motor, and the fifth motor is used to drive the reagent needle to move.

[0042] To achieve one of the above objects of the invention, the present invention further provides a flow cytometer, including the flow sample pretreatment system as described above, and a fully automatic test and analysis system.

[0043] Compared with the prior art, the flow sample pretreatment system of the present invention includes a rack module, a sample arm module, a sample rack module, a sample puncture module, a reagent arm module, a reagent rack module, a reagent needle module, a centrifuge module, a clamping module, an oscillation vortex module and a control module. The sample puncture module is used to puncture the sample container, the reagent needle module is used to add the reagent in the reagent container to the sample container to form a second-generation sample container, the centrifuge module performs centrifugal treatment, the oscillation vortex module performs oscillation treatment, and the control module is used to provide control and / or operation for all components of the flow sample pretreatment system of the present invention. In this way, through automatic sample and reagent transfer, automatic mixing, centrifugation, oscillation and other treatments, a fully automated flow sample pretreatment process is realized, which greatly improves the test efficiency, reduces the test error, and has high safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0045] Figure 1 It is a schematic diagram from one perspective of a flow sample preprocessing system according to an embodiment of the present invention.

[0046] Figure 2 yes Figure 1 Top view of the flow sample pretreatment system.

[0047] Figure 3 yes Figure 1 Schematic diagram of another perspective of the flow sample pretreatment system.

[0048] Figure 4 yes Figure 1 Schematic diagram of the sample rack module in the flow sample pretreatment system.

[0049] Figure 5 yes Figure 1 Schematic diagram of the first state of the sample puncture module in the flow sample pretreatment system.

[0050] Figure 6 yes Figure 5Schematic diagram of the sample puncture module from another perspective.

[0051] Figure 7 yes Figure 1 Schematic diagram of the second state of the sample puncture module in the flow sample pretreatment system.

[0052] Figure 8 yes Figure 7 Schematic diagram of the sample puncture module from another perspective.

[0053] Fig. 9 yes Figure 1 Schematic diagram of the third state of the sample puncture module in the flow sample pretreatment system.

[0054] Fig.10 yes Fig. 9 Schematic diagram of the sample puncture module from another perspective.

[0055] Fig.11 yes Figure 1 Schematic diagram of the centrifuge cover of the centrifuge module in the flow sample pretreatment system.

[0056] Fig.12 yes Figure 1 Schematic diagram of the angle rotor of the centrifuge in the centrifuge module in the flow sample pretreatment system.

[0057] Fig.13 yes Figure 1 A partial schematic diagram of an angle rotor of a centrifuge in a centrifuge module in a flow sample pretreatment system.

[0058] Fig.14 yes Figure 1 Another partial schematic diagram of the angular rotor of the centrifuge of the centrifuge module in the flow sample pretreatment system.

[0059] Fig.15 yes Figure 1 Schematic diagram of the clamping module and reagent needle module in the flow sample pretreatment system.

[0060] Fig.16 yes Figure 1 Schematic diagram of the clamping module in the flow sample pretreatment system.

[0061] Fig.17 yes Figure 1 Partial schematic diagram of the reagent needle module in the flow sample pretreatment system.

[0062] Fig.18 yes Figure 1 Schematic diagram of the oscillating vortex module in the flow sample pretreatment system.

[0063] Fig.19 yes Figure 1Partial cross-sectional diagram of the oscillating vortex module in the flow sample pretreatment system.

[0064] Fig. 20 is along Fig.19 Schematic diagram of a partial cross section along the dividing line AA.

[0065] Fig.21 yes Fig. 20 A simplified schematic diagram of . DETAILED DESCRIPTION

[0066] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0067] Please refer to Figures 1 to 21 , the flow sample preprocessing system of the present invention is described.

[0068] like Figures 1 to 3 As shown, the flow sample pretreatment system includes a rack module 11, a sample arm module 12, a sample rack module 13, a sample puncture module 14, a reagent arm module 15, a reagent rack module 16, a reagent needle module 17, a centrifuge module 18, a clamping module 19, an oscillation vortex module 20 and a control module. The sample arm module 12 is movably mounted on the rack module 11, the sample rack module 13 is arranged below the sample arm module 12, and is used to accommodate a plurality of sample containers 21 and drive the plurality of sample containers 21 to flip, and the sample puncture module 14 is movably mounted on the sample arm module 12, and the sample puncture module 14 includes a sample needle 22, and the sample needle 22 is used to puncture the sample container 21. The reagent arm module 15 can also be movably installed on the rack module 11 and is parallel to the sample arm module 12. The reagent rack module 16 is arranged below the reagent arm module 15 for accommodating at least one reagent container 23. The reagent needle module 17 can be movably installed on the reagent arm module 15. The reagent needle module 17 includes a reagent needle 24. The reagent needle 24 is used to add the reagent in the reagent container 23 to the sample container 21 to form a second-generation sample container 25. In addition, the clamping module 19 can also be movably installed on the reagent arm module 15 for grabbing and transferring the second-generation sample container 25.

[0069] The centrifuge module 18 is disposed beside the sample rack module 13 and / or the reagent rack module 16, and is used to accommodate the second-generation sample container 25 and perform centrifugal treatment on the second-generation sample container 25. The oscillation vortex module 20 is disposed beside the centrifuge module 18, and is used to accommodate the second-generation sample container 25 and perform oscillation treatment on the second-generation sample container 25. The control module is used to provide control and / or operation for all components of the flow sample pretreatment system of the present invention, so as to realize full automation of the flow sample pretreatment process.

[0070] As described above, the flow sample pretreatment system of the present invention realizes a fully automated flow sample pretreatment process by automatically transferring sample reagents, automatically performing mixing, centrifugation, shaking and other processes, greatly improving test efficiency, reducing test errors, and having high safety.

[0071] In the embodiment of the present invention, the rack module 11 adopts a gantry structure, which avoids deformation of the arm group when the cantilever structure of the arm group is loaded with a large load, and ensures the service life of the X-direction guide rail of the arm group. Preferably, a slide rail structure is provided on the cantilever on one side of the rack module 11 for the sliding of the sample arm module 12 and the reagent arm module 15. At the same time, a slide rail is also provided on the sample arm module 12 for the sliding of the sample puncture module 14, and a slide rail is also provided on the reagent arm module 15 for the sliding of the reagent needle module 17 and the clamping module 19. This is because the slide rail structure is simple and easy to move, but the present invention is not limited to this, and other methods that can achieve movable installation are also applicable.

[0072] The flow sample pretreatment system of the present invention further includes a needle washing pool module, which includes a sample needle washing pool 26 and a reagent needle washing pool 27, wherein the sample needle washing pool 26 is used to wash the sample needle 22, and the reagent needle washing pool 27 is used to wash the reagent needle 24. The two needle washing pools can be arranged nearby in the corresponding activity area, for example, the sample needle washing pool 26 is arranged next to the centrifuge module 18, and the reagent needle washing pool 27 is arranged next to the oscillation vortex module 20. The provision of two separate needle washing pools avoids cross contamination caused by mixed washing of the sample needle 22 and the reagent needle 24.

[0073] Next, each module is described in detail with reference to the accompanying drawings.

[0074] like Figure 4 As shown, the sample rack module 13 includes a sample rack 28, a gear 29 and a first motor 30, wherein the sample rack 28 is used to hold a plurality of sample containers 21, the gear 29 is connected to one end of the sample rack 28, and the first motor 30 is arranged below the gear 29 to drive the gear 29 to drive the sample rack 28 to flip as a whole. Compared with the time-consuming disadvantage of the traditional U-shaped sample rack that shakes the single tubes one by one, the sample rack 28 of the present invention adopts an overall shaking sample rack, which is time-saving and efficient. In a more preferred embodiment, the gear 29 adopts a POM gear transmission to reduce abnormal rotation noise.

[0075] In addition, if Figure 3As shown, the flow sample pretreatment system further includes an identification module 31, which is disposed at the other end of the sample rack 28 and is used to read the barcode on the sample container 21 and / or the second-generation sample container 25. In other words, when the user loads different containers, the identification module 31 automatically reads the barcode on the container and identifies the sample information, saving the time of manually entering the sample, thus realizing the automatic sample information entry of the flow sample pretreatment system. Preferably, the identification module 31 is a barcode scanner.

[0076] Please continue to refer to Figures 5 to 10 The sample puncture module 14 further includes a gripping portion 32, a second motor 33 and a third motor 34, wherein the second motor 33 is used to drive the gripping portion 32 to move so as to grip the sample container 21, and the third motor 34 is used to drive the sample needle 22 to puncture the sample container 21 when the gripping portion 32 grips the sample container 21, wherein the second motor 33 and the third motor 34 are arranged in parallel, the gripping portion 32 and the sample needle 22 move in the same direction, and the sample needle 22 is arranged directly above the gripping portion 32. Specifically, as Figure 5 and Figure 6 As shown, when performing sample puncture, the sample puncture module 14 moves to the top of the sample container 21 to be punctured, so that the gripping portion 32 is aligned with the tube mouth of the sample container 21; Figure 7 and Figure 8 As shown, the second motor 33 drives the holding portion 32 to move downward to hold the nozzle of the sample container 21; at this time, as shown in FIG. Fig. 9 and Fig.10 As shown, the third motor 34 drives the sample needle 22 to move downward to puncture the sample container 21. That is to say, the holding design in the sample puncture module 14 of this embodiment not only prevents the sample container 21 from being offset during puncture, but also ensures that the sample needle 22 does not bend when subjected to force. At the same time, the holding and puncture are driven by a dual motor to ensure that the sample container 21 is not brought out during the push back process of the sample needle 22 after the puncture.

[0077] Please refer to Figures 11 to 14The centrifuge module 18 includes a centrifuge 35, a thickened chassis 36 and an unbalanced protection module, wherein the thickened chassis 36 is used to carry the centrifuge 35, ensuring the stability of the centrifuge 35 during operation, and the addition of the unbalanced protection module ensures that the centrifuge 35 gradually slows down when reaching the critical point of unbalance, further ensuring the service life of the centrifuge 35 and the safety of related operators. The centrifuge 35 includes a cover 37 and an angle rotor 38, the cover 37 is provided with an openable cover 39 and a motor for driving the cover 39 to open and close, and the annular surface of the angle rotor 38 is provided with a plurality of container sockets 40 for inserting a plurality of second-generation sample containers 25, and the plurality of container sockets 40 are hinged and fixed to form a ring. The centrifuge 35 of the present invention adopts a self-switching angle rotor. When the centrifuge 35 reaches a certain speed, the centrifuge 35 automatically switches to the angle rotor 38. After the centrifugation is completed, the container holder returns to a vertical state due to its own gravity, so that the clamping module 19 can transfer the second-generation sample container 25 in the centrifuge 35. The unique centrifuge balancing algorithm of the whole machine ensures that the centrifugation experiment can be carried out smoothly under the odd number of samples.

[0078] Please refer to Fig.15 and Fig.16 The clamping module 19 includes a plurality of clamps 41 and a fourth motor 42. The plurality of clamps 41 surround and form an automatically adjustable grasping space to grasp the second-generation sample container 25. The fourth motor 42 is used to drive the plurality of clamps 41 to move up, down, left, right, forward, backward and rotate. Specifically, the clamps 41 are 360-degree self-rotating electric clamps with automatic torque adjustment, which can grasp different reaction containers, such as flow tubes, EP tubes, and vacuum blood collection tubes. At the same time, the grasping force is adjusted according to the different containers grasped. After grasping the container, it can rotate automatically to facilitate the identification module 31 to scan the code. Please refer to Fig.15 and Fig.17 The reagent needle module 17 further includes a fifth motor 43, which is used to drive the reagent needle 24 to add the reagent in the reagent container 23 to the sample container 21 to form a second-generation sample container 25. Fig.17 As shown, in this embodiment, the reagent needle module 17 includes two reagent needles and two corresponding motors, and can be designed with proper consideration of the efficiency and type differences of reagent addition, which is not limited by the present invention. Preferably, the clamping module 19 and the reagent needle module 17 are connected as one, for example, they are respectively arranged on both sides of the reagent arm module 15 and move simultaneously.

[0079] Please refer to Figures 18 to 21, the oscillating vortex module 20 includes an oscillating device 44, a clamping device 45 and an oscillating vortex motor, and the oscillating vortex motor is used to drive the oscillating device 44 to oscillate and vortex the second-generation sample container 25, and drive the clamping device 45 to clamp and release. Specifically, the clamping device 45 includes a chassis 46, a collar 47 and an annular gasket 48, and the collar 47 is movably hinged to the chassis 46 through a hinge 49. The end of the hinge 49 can also be designed with a spring 50, for example, and the annular gasket 48 is fixed on the collar 47 so that the second-generation sample container 25 passes through the annular gasket 48 and the collar 47 in sequence. When the plurality of clamps 41 transfer the second-generation sample container 25 to the collar 47, the oscillating vortex motor drives the collar 47 to rotate to drive the annular gasket 48 to contract, thereby clamping and releasing the second-generation sample container 25. The oscillating vortex module 20 of this embodiment adopts a single motor to drive the oscillating vortex, and has an automatic clamping function. The entire module can be pulled out, which is convenient for users to replace consumables.

[0080] The present invention also provides a flow cytometer, comprising the flow sample pretreatment system and the fully automatic test analysis system. The sample processed by the flow sample pretreatment system is placed on the sample rack 51 to be loaded, and then transferred to the test analysis system for automatic analysis and testing, thereby achieving full automation of the entire flow cytometer.

[0081] In summary, the flow sample pretreatment system of the present invention includes a rack module, a sample arm module, a sample rack module, a sample puncture module, a reagent arm module, a reagent rack module, a reagent needle module, a centrifuge module, a clamping module, an oscillation vortex module and a control module. The sample puncture module is used to puncture the sample container, the reagent needle module is used to add the reagent in the reagent container to the sample container to form a second-generation sample container, the centrifuge module performs centrifugal treatment, the oscillation vortex module performs oscillation treatment, and the control module is used to provide control and / or operation for all components of the flow sample pretreatment system of the present invention. In this way, through automatic sample and reagent transfer, automatic mixing, centrifugation, oscillation and other treatments, a fully automated flow sample pretreatment process is realized, which greatly improves the test efficiency, reduces the test error, and has high safety.

[0082] It should be understood that although this specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each implementation mode may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

[0083] The present invention is explained from the viewpoints of purpose of use, efficiency, progress and novelty, and its practical progress meets the functional enhancement and use requirements emphasized by the Patent Law. The above description and drawings of the present invention are only preferred embodiments of the present invention, and are not intended to limit the present invention. Therefore, all structures, devices, features, etc. that are similar or identical to the present invention, that is, all equivalent replacements or modifications made according to the scope of the patent application of the present invention, should fall within the scope of protection of the patent application of the present invention.

Claims

1. A flow sample preprocessing system, characterized in that: The flow sample preprocessing system comprises: A rack module, wherein the rack module adopts a gantry structure; A sample arm module is movably mounted on the rack module; A sample rack module, disposed below the sample arm module, for accommodating a plurality of sample containers and driving the plurality of sample containers to flip; A sample puncture module is movably mounted on the sample arm module, and the sample puncture module comprises a sample needle, and the sample needle is used to puncture the sample container; A reagent arm module is movably mounted on the rack module and is parallel to the sample arm module; A reagent rack module, disposed below the reagent arm module, for accommodating at least one reagent container; A reagent needle module is movably mounted on the reagent arm module, the reagent needle module comprises a reagent needle, and the reagent needle is used to add the reagent in the reagent container to the sample container to form a second-generation sample container; A centrifuge module, disposed beside the sample rack module and / or the reagent rack module, for accommodating the second-generation sample container and performing centrifugal treatment on the second-generation sample container; A clamping module, movably mounted on the reagent arm module, for grabbing and transferring the second-generation sample container; An oscillation vortex module, disposed beside the centrifuge module, for accommodating the second-generation sample container and performing oscillation treatment on the second-generation sample container; and The control module is used to control and / or operate all the elements of the flow sample preprocessing system to realize the automation of the flow sample preprocessing process.

2. The flow sample preprocessing system according to claim 1, characterized in that: The flow sample pretreatment system further comprises a needle washing pool module, and the needle washing pool module comprises a sample needle washing pool and a reagent needle washing pool.

3. The flow sample preprocessing system according to claim 1, characterized in that: The sample rack module comprises: A sample rack, used for holding a plurality of the sample containers; a gear connected to one end of the sample holder; and The first motor is arranged below the gear to drive the gear to drive the sample rack to flip.

4. The flow sample preprocessing system according to claim 3, characterized in that: The flow sample preprocessing system further comprises an identification module, which is disposed at the other end of the sample rack and is used to read the barcode on the sample container and / or the second-generation sample container.

5. The flow sample preprocessing system according to claim 1, characterized in that: The sample puncture module also includes: Grip part; A second motor, used for driving the holding portion to move so as to hold the sample container; and The third motor is used for driving the sample needle to puncture the sample container when the holding portion holds the sample container.

6. The flow sample preprocessing system according to claim 1, characterized in that: The centrifuge module comprises: A centrifuge, comprising a cover body and an angle rotor, wherein a plurality of container insertion holes for inserting the second-generation sample containers are provided on the annular surface of the angle rotor; A thickened bottom plate for carrying the centrifuge; and The unbalance protection module is used to gradually reduce the speed of the centrifuge when it reaches a critical point of unbalance.

7. The flow sample preprocessing system according to claim 1, characterized in that: The clamping module comprises: A plurality of grippers, surrounding and forming an automatically adjustable gripping space for gripping the second-generation sample container; and The fourth motor is used to drive the plurality of clamps to move and rotate.

8. The flow sample preprocessing system according to claim 1, characterized in that: The oscillating vortex module comprises: Oscillating device; A clamping device, the clamping device comprising: Chassis; A collar, movably hinged to the chassis via a hinge; an annular gasket fixed on the collar so that the second-generation sample container passes through the annular gasket and the collar in sequence; and The oscillating vortex motor is used to drive the oscillating device to oscillate and vortex the second-generation sample container, and drive the ring to rotate to drive the annular gasket to contract, thereby clamping and loosening the second-generation sample container.

9. The flow sample preprocessing system according to claim 1, characterized in that: The reagent needle module also includes a fifth motor, and the fifth motor is used to drive the reagent needle to move.

10. A flow cytometer, characterized in that: It comprises a flow sample preprocessing system as described in any one of claims 1 to 9, and a fully automatic test and analysis system.

Images