An automated detection device that integrates biogas aerosol sampling and nucleic acid detection

Abstract

The application discloses an automatic detection device integrating biological aerosol sampling and nucleic acid detection, wherein a sampling and feeding unit and a nucleic acid detection unit of the automatic detection device are both installed on the top of a chip conveying unit; the sampling and feeding unit is used for adding sampling liquid to the chip; the chip conveying unit is used for conveying the chip added with the sampling liquid to the nucleic acid detection unit for detection and moving the detected chip away from the nucleic acid detection unit; the nucleic acid detection unit is used for detecting the sampling liquid added to the chip; and an automatic control unit controls the actions of the sampling and feeding unit, the chip conveying unit and the nucleic acid detection unit and acquires the detection result of the nucleic acid detection unit. The automatic detection device integrates biological aerosol sampling technology and nucleic acid detection technology, can improve the biological aerosol detection efficiency, reduce manual work, can be operated without a professional laboratory and is suitable for most scenes.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological aerosol monitoring, and particularly relates to an automatic detection device integrating biological aerosol sampling and nucleic acid detection. BACKGROUND

[0002] In recent years, SARS (Severe acute respiratory syndrome coronavirus), Ebola, influenza A, and COVID-19 have spread globally, causing great harm to human life and health. Aerosol is an important way for viruses and bacteria to spread. Biological aerosol detection can provide scientific basis and effective technical support for epidemic prevention and control.

[0003] The traditional biological aerosol detection method is to collect biological aerosol into culture medium, liquid, filter membrane and other media by a biological aerosol sampler, and then to detect the collected sample by culture method, immunological technology, nucleic acid technology and other schemes, so as to determine whether there is a certain microorganism in the ambient air. The method has the advantages of being able to monitor the types and concentrations of microorganisms, but has the disadvantages of complex sampling and detection operation, requiring professional personnel to operate, requiring a professional laboratory, and taking a long time, which is poor in timeliness. Therefore, the existing biological aerosol detection has the problems of separation of sampling and detection and low automation. SUMMARY

[0004] Therefore, the present application provides an automatic detection device integrating biological aerosol sampling and nucleic acid detection. The automatic detection device uses nucleic acid detection technology for biological aerosol detection, integrates biological aerosol sampling technology and nucleic acid detection technology, can improve the efficiency of biological aerosol detection, reduce manual work, and can be operated without a professional laboratory, which is suitable for most scenes.

[0005] The present application adopts the following specific technical solutions:

[0006] The automatic detection device integrating biological aerosol sampling and nucleic acid detection includes a sampling and sample feeding unit, a chip conveying unit, a nucleic acid detection unit, and an automatic control unit.

[0007] The sampling and sample feeding unit and the nucleic acid detection unit are both installed on the top of the chip conveying unit. The sampling and sample feeding unit is used to add sampling liquid to the chip. The chip conveying unit is used to convey the chip with added sampling liquid to the nucleic acid detection unit for detection, and to move the detected chip away from the nucleic acid detection unit. The nucleic acid detection unit is used to detect the sampling liquid added to the chip.

[0008] The automatic control unit controls the sampling and feeding unit, the chip conveying unit and the nucleic acid detection unit to operate and obtain the detection result of the nucleic acid detection unit.

[0009] Further, the nucleic acid detection unit is provided with a chip inlet and outlet and comprises a conveying module, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module.

[0010] The conveying module conveys the detection reagent kit between the nucleic acid extraction module, the nucleic acid amplification module and the fluorescence detection module for conveying the sample liquid required in the nucleic acid extraction, amplification and detection process.

[0011] Further, the chip conveying unit comprises a chip feeding and discharging module and a chip conveying module located at the bottom of the chip feeding and discharging module.

[0012] The chip feeding and discharging module is used to push the chip to be detected through the chip inlet and outlet into the nucleic acid detection unit and to carry the detected chip in the nucleic acid detection unit to the chip conveying module.

[0013] The chip conveying module is used to convey the chip.

[0014] Further, the chip conveying module is a chain conveying mechanism comprising a stepping motor, a transmission chain in transmission connection with the stepping motor and a chain straight ear fixedly installed on the transmission chain.

[0015] The chain straight ear is used to push the chip to move.

[0016] Further, a chip to be detected card slot for storing the chip to be detected is arranged on one side of the chip conveying module and a detected chip card slot for storing the detected chip is arranged on the other side; a chip rack for quickly taking and placing the chip is arranged in each of the chip to be detected card slot and the detected chip card slot.

[0017] The nucleic acid detection unit is located between the chip to be detected card slot and the detected chip card slot.

[0018] An electric push rod is arranged at the bottom of the detected chip card slot, which is used to push the detected chip into the detected chip card slot and to stack the detected chip.

[0019] A pawl is arranged in the detected chip card slot for restraining the detected chip from falling.

[0020] Further, a horizontal electromagnetic baffle is arranged between the nucleic acid detection unit and the chip to be detected card slot.

[0021] The electromagnetic baffle is used for fixing the front and back directions of the chip to be detected, and preventing the chip to be detected from being skewed in the conveying process.

[0022] Further, a drive chain zero point sensor and a drive chain positioning sensor are arranged below the upper chain of the drive chain, and the drive chain zero point sensor and the positioning sensor are used for detecting the position of the chip to be detected.

[0023] Further, the chip in-out module comprises a chip in-out sliding stage, a suction cup limit sensor, a suction cup zero point sensor, a mounting plate, a suction cup, a suction cup air pump and a negative pressure switch.

[0024] The chip in-out sliding stage is capable of moving in the front and back directions, and is installed at one side of the nucleic acid detection unit, and the front end is provided with a suction cup limit sensor for detecting the insertion depth of the chip to be detected, and the rear end is provided with a suction cup zero point sensor for determining the zero point position of the chip in-out sliding stage.

[0025] The suction cup is fixedly installed on the chip in-out sliding stage through the mounting plate.

[0026] The suction cup and the suction cup air pump are connected through a silica gel tube, and a negative pressure switch is connected in the silica gel tube.

[0027] Further, the sampling and sample feeding unit comprises a liquid storage bottle, a liquid supplementing pump, a sampling bottle, a sampling head, an air pump, a liquid outlet pump, a sliding stage, a mechanical positioning clamp and a liquid delivery needle.

[0028] The sliding stage is capable of moving in the vertical direction, and is installed above the drive chain, and the mechanical positioning clamp and the liquid delivery needle are fixedly installed on the sliding block of the sliding stage.

[0029] The mechanical positioning clamp is used for positioning the chip.

[0030] The liquid delivery needle is used for piercing the chip, adding sampling liquid and discharging waste liquid to the waste liquid bottle.

[0031] The liquid supplementing outlet of the liquid storage bottle, the liquid supplementing pump and the sampling bottle are connected through silica gel tubes in sequence.

[0032] The air pump is connected with the air outlet of the sampling head, and is used for forming negative pressure in the sampling bottle, so that aerosol particles enter the sampling bottle for sampling.

[0033] The liquid outlet of the sampling bottle, the liquid outlet pump and the liquid delivery needle are connected through silica gel tubes in sequence.

[0034] The axis of the liquid delivery needle coincides with the axis of the silica gel plug of the chip to be detected at the sampling liquid adding position.

[0035] Further, the sampling and feeding unit further comprises a waste liquid bottle, an infusion needle zero sensor and an infusion needle limiting sensor.

[0036] The waste liquid bottle is located below the infusion needle and is used for storing the remaining sampling liquid in the sampling bottle.

[0037] When the infusion needle is in the maximum displacement, the infusion needle is located in the bottle mouth of the waste liquid bottle.

[0038] The infusion needle zero sensor is located on the top of the sliding table and is used for determining the zero point position of the infusion needle.

[0039] The infusion needle limiting sensor is installed on the bottom of the sliding table and is used for limiting the infusion needle.

[0040] Beneficial effects:

[0041] The automatic detection device integrates the biological aerosol sampling technology and the nucleic acid detection technology, can improve the biological aerosol detection efficiency, reduces the labor, can be operated without professional laboratory, is suitable for most scenes, can study and explore the aerosol propagation law through the daily monitoring of the biological aerosol, can provide technical support for the epidemic prevention and control through the early warning of the propagation of pathogenic microorganisms. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a schematic structural diagram of the automatic detection device;

[0043] Figure 2 It is a schematic structural diagram of the nucleic acid detection unit of the automatic detection device;

[0044] Figure 3 It is a schematic structural diagram of the sampling and feeding unit of the automatic detection device;

[0045] Figure 4 It is a schematic structural diagram of the chip conveying module of the automatic detection device;

[0046] Figure 5 It is a schematic structural diagram of the chip conveying module of the automatic detection device;

[0047] Wherein, 1-sampling sending unit, 2-chip conveying unit, 3-nucleic acid detection unit, 4-automatic control unit, 5-chip in-out module, 6-chip conveying module, 7-detected chip card slot, 8-chip to be detected card slot, 9-chip to be detected, 10-liquid storage bottle, 11-liquid supplementing pump, 12-sampling bottle, 13-air pump, 14-liquid outlet pump, 15-sliding table, 16-mechanical positioning clamp, 17-liquid infusion needle, 18-transmission chain, 19-waste liquid bottle, 20-liquid infusion needle zero sensor, 21-liquid infusion needle limiting sensor, 22-chip rack, 23-chain straight ear, 24-transmission chain zero sensor, 25-transmission chain positioning sensor, 26-stepping motor, 27-electric push rod, 28-pawl, 29-detected chip, 30-electromagnetic baffle, 31-chip in-out sliding table, 32-suction cup limiting sensor, 33-suction cup zero sensor, 34-mounting plate, 35-suction cup, 36-suction cup air pump, 37-negative pressure switch, 41-conveying module, 42-nucleic acid extraction module, 43-nucleic acid amplification module, 44-fluorescence detection module DETAILED DESCRIPTION

[0048] The application will be described in detail below with reference to the drawings and examples.

[0049] REFERENCE Figure 1 The application provides an automatic detection device integrating biological aerosol sampling and nucleic acid detection, which comprises a sampling sending unit 1, a chip conveying unit 2, a nucleic acid detection unit 3 and an automatic control unit 4. The sampling sending unit 1 and the nucleic acid detection unit 3 are both installed on the top of the chip conveying unit 2. The sampling sending unit 1 is used for adding sampling liquid to the chip. The chip conveying unit 2 is used for conveying the chip added with the sampling liquid to the nucleic acid detection unit 3 for detection and moving the detected chip away from the nucleic acid detection unit 3. The nucleic acid detection unit 3 is used for detecting the sampling liquid added to the chip. The automatic control unit 4 controls the actions of the sampling sending unit 1, the chip conveying unit 2 and the nucleic acid detection unit 3 and obtains the detection result of the nucleic acid detection unit 3. The chip can be a microfluidic chip.

[0050] The automatic detection device comprises a sampling and feeding unit 1 for sampling and feeding sample liquid to the chip, a chip conveying unit 2 for conveying the chip, a nucleic acid detection unit 3 for detecting the sample liquid in the chip, and an automatic control unit 4 for controlling the operation of the units according to a predetermined program. The nucleic acid detection technology is used for biological aerosol detection through the sampling and feeding unit 1, the chip conveying unit 2 and the nucleic acid detection unit 3. The biological aerosol sampling technology and the nucleic acid detection technology are integrated, the sampling and nucleic acid detection of the biological aerosol are integrated and automated, the detection of the aerosol is realized, the biological aerosol detection efficiency is improved, the manual operation is reduced, the professional laboratory is no longer needed, and the device is suitable for most scenes. The biological aerosol detection information is uploaded in real time through the automatic control unit 4, the daily monitoring of the biological aerosol can be researched and explored, the aerosol transmission rule can be researched and explored, the transmission of the pathogenic microorganism is warned, and technical support is provided for epidemic prevention and control.

[0051] In a specific embodiment, as shown in Figure 2 The nucleic acid detection unit 3 is provided with a chip inlet and outlet, and comprises a conveying module 41, a nucleic acid extraction module 42, a nucleic acid amplification module 43 and a fluorescence detection module 44. The conveying module 41 conveys the detection reagent box between the nucleic acid extraction module 42, the nucleic acid amplification module 43 and the fluorescence detection module 44, and is used for conveying the sample liquid required in the nucleic acid extraction, amplification and detection processes.

[0052] The nucleic acid detection unit 3 can integrate the sample pretreatment, nucleic acid amplification and detection processes on a microfluidic chip, and realize the integration and automation of the sample pretreatment, nucleic acid amplification and detection through the nucleic acid detection integrated system. The sample liquid is detected in the nucleic acid detection chip, the sample reaction pool in the chip is sealed with the microflow channel and the outside world, and the pollution of nucleic acid to the environment is avoided. The nucleic acid detection chip is designed to be fully automatic, and manual intervention is not needed.

[0053] Further, the chip conveying unit 2 comprises a chip feeding and discharging module 5 and a chip conveying module 6 located at the bottom of the chip feeding and discharging module 5. The chip feeding and discharging module 5 is used to push the to-be-detected chip 9 through the chip inlet and outlet into the nucleic acid detection unit 3, and to carry the detected chip 29 in the nucleic acid detection unit 3 to the chip conveying module 6. The chip conveying module 6 is used to convey the chip. As shown in Figure 4As shown, the chip conveying module 6 can be a chain conveying mechanism, including a stepping motor 26, a transmission chain 18 in driving connection with the stepping motor 26, and a chain straight ear 23 fixedly installed on the transmission chain 18; the chain straight ear 23 is used to push the chip to move. A to-be-inspected chip card slot 8 for storing the to-be-inspected chip 9 is arranged on one side of the chip conveying module 6, and a detected chip card slot 7 for storing the detected chip 29 is arranged on the other side; the lower surface of the to-be-inspected chip card slot 8 and the lower surface of the detected chip card slot 7 are coincident with the lower surface of the chip entrance and exit of the nucleic acid detection unit 3; a chip rack 22 for quickly taking and placing the chip is arranged in the to-be-inspected chip card slot 8 and the detected chip card slot 7; the chip rack 22 facilitates taking out the detected chip 29 from the detected chip card slot 7 at one time or placing a plurality of to-be-inspected chips 9 into the to-be-inspected chip card slot 8 at one time; the nucleic acid detection unit 3 is located between the to-be-inspected chip card slot 8 and the detected chip card slot 7; an electric push rod 27 is arranged at the bottom of the detected chip card slot 7, and the electric push rod 27 is used to push the detected chip 29 into the detected chip card slot 7 to stack the detected chip 29; a pawl 28 for restraining the detected chip 29 from falling is arranged in the detected chip card slot 7; the pawl 28 can restrain the detected chip 29 in the detected chip card slot 7; the electric push rod 27 continuously pushes the detected chip 29 up and down, and the detected chip 29 can be stacked.

[0054] As shown in Figure 4 and Figure 5 A horizontal electromagnetic baffle 30 is arranged between the nucleic acid detection unit 3 and the to-be-inspected chip card slot 8; the electromagnetic baffle 30 is used to fix the front and back directions of the to-be-inspected chip 9, and prevent the to-be-inspected chip 9 from being skewed in the conveying process. A transmission chain zero point sensor 24 and a transmission chain positioning sensor 25 are arranged below the upper circle chain of the transmission chain 18; the transmission chain zero point sensor 24 and the transmission chain positioning sensor 25 are used to detect the position of the to-be-inspected chip 9.

[0055] The conveying process of the chip conveying unit 2 is as follows: the initial state is that the transmission chain zero point sensor 24 is triggered, the chain straight ear 23 is located at the right side of the to-be-inspected chip 9, after the chip conveying process is started, the stepping motor 26 is powered to rotate and drive the transmission chain 18 to operate counterclockwise, the to-be-inspected chip 9 can directly fall on the transmission chain 18 by gravity, the chain straight ear 23 pushes the to-be-inspected chip 9 to move left, the electromagnetic baffle 30 is used to prevent the to-be-inspected chip 9 from being skewed during the conveying process, when the transmission chain positioning sensor 25 is triggered, the stepping motor 26 stops rotating, the to-be-inspected chip 9 is aligned with the chip inlet and outlet of the nucleic acid detection unit 3, after the sampling and sampling electromagnetic baffle 30 suction, carding, electromagnetic baffle 30 disconnection, detection, and carding process are completed, the stepping motor 26 is started, the chain straight ear 23 pushes the inspected chip 29 to move left, and after the inspected chip 29 is sent to the lower side of the inspected chip card slot 7, the electric push rod 27 moves upward to push the inspected chip 29 into the inspected chip card slot 7 for stacking, and the pawl 28 limits the inspected chip 29 in the inspected chip card slot 7.

[0056] Specifically, as shown in Figure 5 The chip inlet and outlet module 5 includes a chip inlet and outlet sliding table 31, a suction cup limit sensor 32, a suction cup zero position sensor 33, a mounting plate 34, a suction cup 35, a suction cup air pump 36, and a negative pressure switch 37. The chip inlet and outlet sliding table 31 can move in the front-rear direction and is installed on the left side of the nucleic acid detection unit 3. The front end of the chip inlet and outlet sliding table 31 is provided with a suction cup limit sensor 32 for detecting the insertion depth of the to-be-inspected chip 9. The suction cup limit sensor 32 is used to detect the insertion depth of the to-be-inspected chip 9. The rear end of the chip inlet and outlet sliding table 31 is provided with a suction cup zero position sensor 33 for determining the zero position of the chip inlet and outlet sliding table 31. The suction cup zero position sensor 33 is used to determine the zero position of the chip inlet and outlet sliding table 31. When the chip inlet and outlet sliding table 31 is at the maximum displacement, the to-be-inspected chip 9 coincides with the detection position of the nucleic acid detection unit 3. The suction cup 35 is fixedly installed on the chip inlet and outlet sliding table 31 through the mounting plate 34. The horizontal center line of the suction cup 35 is flush with the horizontal center line of the to-be-inspected chip 9, which functions to push the to-be-inspected chip 9 into the nucleic acid detection unit 3 and pull the inspected chip 29 out of the nucleic acid detection unit 3. The suction cup 35 and the suction cup air pump 36 are connected through a silica gel tube, and the negative pressure switch 37 is connected in the silica gel tube. When the suction cup 35 sucks the chip, the negative pressure switch 37 is triggered.

[0057] The working process of the chip in-out module 5 is as follows: the initial state is that the suction cup zero position sensor 33 is triggered, the chip 9 to be detected is filled with sampling liquid, the filling sampling liquid slide table 15 is returned to zero, after the chip in-out program is started, the electromagnetic baffle 30 is energized and attracted, the chip in-out slide table 31 is started and drives the mounting plate 34 and the suction cup 35 to push the chip 9 to be detected forward, the chip 9 to be detected is inserted into the nucleic acid detection unit 3, after the suction cup limit position sensor 32 is triggered, the electromagnetic baffle 30 is de-energized, the chip in-out slide table 31 is reversely operated for a distance, after the nucleic acid detection unit 3 is detected, the nucleic acid detection unit 3 partially exits the detected chip 29, after the card withdrawal signal is received, the suction cup air pump 36 is started, the chip in-out slide table 31 is forwardly operated, when the suction cup 35 is attached to the detected chip 29, the negative pressure switch 37 is triggered, the chip in-out slide table 31 drives the detected chip 29 to be reversely operated through the suction cup 35, the suction cup zero position sensor 33 is triggered to stop, and the chip in-out process is completed.

[0058] As shown in Figure 3 The sampling and sending unit 1 includes a liquid storage bottle 10, a liquid supplementing pump 11, a sampling bottle 12, a sampling head, an air pump 13, a liquid outlet pump 14, a slide table 15, a mechanical positioning clamp 16 and a liquid injection needle 17. The slide table 15 can move in the vertical direction and is installed above a transmission chain 18. The mechanical positioning clamp 16 and the liquid injection needle 17 are fixedly installed on the sliding block of the slide table 15. The mechanical positioning clamp 16 is used for positioning the chip. The liquid injection needle 17 is used for piercing the chip and filling the sampling liquid and discharging the excess sampling liquid into a waste liquid bottle 19 after the sampling liquid is filled into the chip. The liquid storage bottle 10, the liquid supplementing pump 11 and the liquid supplementing port of the sampling bottle 12 are sequentially connected through silica gel tubes. The sampling head covers the opening of the sampling bottle 12. The sampling head has a gas inlet and a gas outlet which are communicated with the sampling bottle 12. The gas inlet is communicated with the atmosphere, and the gas outlet is connected with the air pump 13. The air pump 13 provides power for sampling, so as to form negative pressure in the sampling bottle 12, and aerosol particles enter the sampling bottle 12 for sampling. The liquid outlet of the sampling bottle 12, the liquid outlet pump 14 and the liquid injection needle 17 are sequentially connected through silica gel tubes. The axis of the liquid injection needle 17 coincides with the axis of the silica gel plug of the chip 9 to be detected at the sampling liquid filling position. The sampling and sending unit 1 can further include the waste liquid bottle 19, a liquid injection needle zero position sensor 20 and a liquid injection needle limit position sensor 21. The waste liquid bottle 19 is located below the liquid injection needle 17 and is used for storing the remaining sampling liquid in the sampling bottle 12, such as the sampling liquid except for the sampling liquid input into the chip. When the liquid injection needle 17 is at the maximum displacement, the liquid injection needle 17 is located in the bottle opening of the waste liquid bottle 19. The liquid injection needle zero position sensor 20 is located at the top of the slide table 15 and is used for determining the zero position of the liquid injection needle 17. The liquid injection needle limit position sensor 21 is installed at the bottom of the slide table 15 and is used for limiting the liquid injection needle 17.

[0059] When the sampling and sample feeding unit 1 is working, the liquid supplement pump 11 quantitatively inputs the collection liquid in the liquid storage bottle 10 into the sampling bottle 12, the air pump 13 is started to sample, after sampling is completed, the sliding table 15 moves downward, in the travel of the infusion needle 17 not contacting the chip 9 to be detected, the mechanical positioning clamp 16 completes the right positioning of the chip, then the infusion needle 17 pierces into the chip 9 to be detected, the liquid outlet pump 14 is used to quantitatively add the sampling liquid into the chip 9 to be detected, after adding is completed, the sliding table 15 returns to zero, the remaining sampling liquid is added into the waste liquid bottle 19 by the liquid outlet pump 14 in the chip detection stage.

[0060] The working process of the automatic detection device is as follows: when the liquid storage bottle 10 and the chip 9 to be detected are installed in place, the transmission chain 18, the suction cup 35 and the infusion needle 17 return to zero, the liquid supplement pump 11 is started, a certain amount of liquid is extracted from the liquid storage bottle 10 through the silica gel pipe into the sampling bottle 12, the sampling time can be set through the automatic control unit 4, after sampling is completed, the chip conveying unit 2 transports the chip 9 to be detected to the outside of the chip inlet and outlet of the nucleic acid detection unit 3 through the chain straight ear 23, the sliding table 15 of the sampling and sample feeding unit 1 drives the mechanical positioning clamp 16 and the infusion needle 17 to move downward, in the process that the infusion needle 17 does not contact the chip, the mechanical positioning clamp 16 right positions the chip, so that the chip faces the chip inlet of the nucleic acid detection unit 3, after the chip is right positioned, the head of the infusion needle 17 is inserted into the silica gel plug of the chip to input a certain amount of sampling liquid, after conveying is completed, the sliding table 15 drives the mechanical positioning clamp 16 and the infusion needle 17 to return to zero, then the chip in and out module 5 pushes the chip into the inside of the nucleic acid detection unit 3 for detection, after detection is completed, the nucleic acid detection unit 3 partially exits the detected chip 29, the chip in and out module 5 re-conveys the detected chip 29 to the position of adding the sampling liquid through the suction cup 35, the pipeline of the suction cup 35 is provided with a negative pressure switch 37, the movement state of the detected chip 29 in this process is judged; after the detected chip 29 reaches the position of adding the sampling liquid, the chip conveying unit 2 operates to convey the detected chip 29 to the lower side of the detected chip slot 7, after the detected chip 29 is in place, the electric push rod 27 is started to push the detected chip 29 into the detected chip slot 7, the one-way property of the ratchet pawl 28 is used to limit the detected chip 29 in the detected chip slot 7, thus the detection process is completed.

[0061] The detection result of the nucleic acid detection unit 3 can be transmitted to the automatic control unit 4, and the automatic control unit 4 can transmit the nucleic acid detection information to the server storage through a wired network or a wireless network; the user can remotely view the biological aerosol monitoring information and control the monitoring through a mobile phone or a computer terminal.

[0062] To sum up, the above is only a preferred embodiment of the present application, and is not used to limit the protection scope of the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An automated detection device that integrates biogas aerosol sampling and nucleic acid detection, characterized in that, The sampling and sample feeding unit, the chip conveying unit, the nucleic acid detection unit and the automatic control unit are included. The sampling and sample feeding unit and the nucleic acid detection unit are both installed on the top of the chip conveying unit; the sampling and sample feeding unit is used for adding sampling liquid to the chip; the chip conveying unit is used for conveying the chip added with the sampling liquid to the nucleic acid detection unit for detection and conveying the detected chip away from the nucleic acid detection unit; and the nucleic acid detection unit is used for detecting the sampling liquid added to the chip. The automatic control unit controls the actions of the sampling and sample feeding unit, the chip conveying unit and the nucleic acid detection unit and acquires the detection result of the nucleic acid detection unit. The nucleic acid detection unit is provided with a chip entrance and exit. The chip conveying unit includes a chip entering and exiting module and a chip conveying module located at the bottom of the chip entering and exiting module. The chip entering and exiting module is used for pushing the chip to be detected to enter the nucleic acid detection unit through the chip entrance and exit and conveying the detected chip in the nucleic acid detection unit to the chip conveying module. The chip conveying module is used for conveying the chip. The chip entering and exiting module includes a chip entering and exiting sliding table, a suction cup limit position sensor, a suction cup zero position sensor, a mounting plate, a suction cup, a suction cup air pump and a negative pressure switch. The chip entering and exiting sliding table can move in the front and back directions and is installed on one side of the nucleic acid detection unit; the front end is provided with a suction cup limit position sensor for detecting the insertion depth of the chip to be detected; and the rear end is provided with a suction cup zero position sensor for determining the zero point position of the chip entering and exiting sliding table. The suction cup is fixedly installed on the chip entering and exiting sliding table through the mounting plate; and the horizontal center line of the suction cup is flush with the horizontal center line of the chip to be detected. The suction cup and the suction cup air pump are connected through a silica gel tube, and the negative pressure switch is connected in the silica gel tube.

2. The automated detection device of claim 1, wherein, The nucleic acid detection unit includes a conveying module, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module. The conveying module conveys the detection reagent box between the nucleic acid extraction module, the nucleic acid amplification module and the fluorescence detection module, and is used for conveying the sample liquid required in the nucleic acid extraction, amplification and detection processes.

3. The automated detection device of claim 2, wherein, The chip conveying module is a chain conveying mechanism and includes a stepping motor, a transmission chain in transmission connection with the stepping motor and a chain straight ear fixedly installed on the transmission chain. The chain straight ear is used for pushing the chip to move.

4. The automated detection device of claim 3, wherein, A chip to be detected card slot for storing the chip to be detected is arranged on one side of the chip conveying module, and a detected chip card slot for storing the detected chip is arranged on the other side; and a chip rack for quickly taking and placing the chip is arranged in the chip to be detected card slot and the detected chip card slot. The nucleic acid detection unit is located between the chip to be detected card slot and the detected chip card slot. An electric push rod is arranged at the bottom of the detected chip card slot and is used for pushing the detected chip into the detected chip card slot and stacking the detected chip. A pawl is arranged in the detected chip card slot and is used for restraining the detected chip from falling.

5. The automated detection device of claim 4, wherein, A horizontal electromagnetic baffle is arranged between the nucleic acid detection unit and the chip to be detected card slot. The electromagnetic baffle is used for fixing the front and back directions of the chip to be detected, and preventing the chip to be detected from being skewed in the conveying process.

6. The automated detection device of claim 5, wherein, A drive chain zero point sensor and a drive chain positioning sensor are arranged below the upper chain of the drive chain, and are used for detecting the position of the chip to be detected.

7. The automated detection device of claim 6, wherein, The sampling and feeding unit comprises a waste liquid bottle, a liquid storage bottle, a liquid supplementing pump, a sampling bottle, a sampling head, an air pump, a liquid outlet pump, a sliding table, a mechanical positioning clamp and a liquid delivery needle. The sliding table is movable in the vertical direction, and is installed above the drive chain. The mechanical positioning clamp and the liquid delivery needle are fixedly installed on the sliding block of the sliding table. The mechanical positioning clamp is used for positioning the chip. The liquid delivery needle is used for puncturing the chip, adding sampling liquid and discharging waste liquid into the waste liquid bottle. The liquid supplementing ports of the liquid storage bottle, the liquid supplementing pump and the sampling bottle are connected in sequence by silica gel tubes. The air pump is connected with the air outlet of the sampling head, and is used for forming negative pressure in the sampling bottle, so that aerosol particles enter the sampling bottle for sampling. The liquid outlet of the sampling bottle, the liquid outlet pump and the liquid delivery needle are connected in sequence by silica gel tubes.

8. The automated detection device of claim 7, wherein, The axis of the liquid delivery needle coincides with the axis of the silica gel plug of the chip to be detected at the sampling liquid adding position. The sampling and feeding unit further comprises a liquid delivery needle zero point sensor and a liquid delivery needle limiting sensor. The waste liquid bottle is located below the liquid delivery needle, and is used for storing the remaining sampling liquid in the sampling bottle. When the liquid delivery needle is at the maximum displacement, the liquid delivery needle is located in the bottle mouth of the waste liquid bottle. The liquid delivery needle zero point sensor is located on the top of the sliding table, and is used for determining the zero point position of the liquid delivery needle. The liquid delivery needle limiting sensor is installed on the bottom of the sliding table, and is used for limiting the liquid delivery needle.

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